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Desalting/Focusing MALDI Plate Depiction of On-chip Purification/Focusing
Desalting/Focusing MALDI Plate
 Arti
T. Navare
Office: ES&T L2-110
Office Phone: 404-385-4427
E-mail:
gtg988z@mail.gatech.edu
Education
Jan 2005 - Present Georgia Institute of Technology, Ph.D.
Student, Analytical Chemistry
2001 Wadia
College, University of Pune, Masters in Chemistry
1998 Garware College, Pune, B.S. Chemistry
Research Interests
Dynamic Substrates
for Atmospheric Pressure MALDI
AP/MALDI is a soft ionization technique
suitable for the analysis of labile biological samples and the portable module can be
coupled to different mass analyzers such as Time-of-flight, FT-ICR and
ion trap. When coupled to an ion trap, MSn analysis can be performed on a
biological sample to obtain structure and sequence information.
We are interested in using the advantages
of AP/MALDI technique for peptidomics, specifically for analyzing
mosquito neuropeptides. Being an ambient ionization method, AP/MALDI
suffers from lower sensitivity than the vacuum MALDI
technique. One of the most important factors affecting the sensitivity
is the sample preparation step. Presence of contaminants such as salts
and polymers along with high abundant proteins or peptides can suppress
low abundant analyte signal, contributing to lower quality mass spectral
data. Conventional solid-phase extraction on-tip columns used for sample
clean-up purposes may result in sample loss due to irreversible binding,
or due to irreproducible flow paths. To overcome the problems of sample
loss and possible contamination issues, we are investigating on-chip
purification and sample preparations methods in a compararative study of a
conventional AP/MALDI target plate and a dynamic preconcentration/focusing
target plate.
The dynamic on-chip purification and
preconcentration/focusing target is a gold-coated stainless steel plate
with a self-assembled monolayer (SAM) of alkylthiols with different
wettability and is functionalized with reverse phase packing material
such as C18. The new substrate not only has a capability to purify and
concentrate higher volumes of diluted sample due to its surface
wettability properties but also has binding specificities as a result of
various surface functionalities. We hope that the AP/MALDI technique in
combination with the on-chip sample clean-up strategy will significantly
enhance the sensitivity and the quality of AP/MALDI-MS, and will provide
a cost-effective alternative for routine peptidomics.
Publications
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"On-chip solid-phase
extraction preconcentration/focusing substrates coupled to
atmospheric pressure matrix-assisted laser desorption/ionization ion
trap mass spectrometry for high sensitivity biomolecule analysis",
Arti Navare, Marcela Nouzova, Fernando G. Noriega,
Salvador Hernandez-Martinez, Christoph Menzel, Facundo M.
Fernandez, Rapid Commun. Mass Spectrom., 2009,
23 (4), 477-486.
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"Liquid Chromatography and Ambient Ionization Time-of-flight Mass
Spectrometry for the Analysis of Genuine and Counterfeit
Pharmaceuticals", Facundo M. Fernández, Christina Y. Hampton,
Leonard Nyadong, Arti Navare, Mark Kwasnik in LC/TOF-MS for
Accurate Mass Analysis: Principles and Applications, ed. by I.
Ferrer and E.M. Thurman, Wiley: 2009.
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"Serum biomarker profiling by solid-phase extraction with
particle-embedded micro tips and matrix-assisted laser
desorption/ionization mass spectrometry", Arti Navare, Manshui Zhou,
John McDonald, Fernando G. Noriega, M. Cameron Sullards, and Facundo
M. Fernanadez, Rapid Commun. Mass Spectrom., 2008,
22 (7), 997-1008.
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"Biochemical,
molecular and functional characterization of PISCF-allatostatin, a
regulator of juvenile hormone biosynthesis in the Aedes aegypti
mosquito", Salvador Hernandez-Martinez, Yiping Li, Facundo Fernández,
Jaime G. Mayoral, Pantelis Topalis, Horacio Priestap, Mario Perez,
Arti Navare and Fernando G. Noriega, J. Biol. Chem.,
2006, 281(45), 34048-34055.
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