******************************************************************************
From: "Peter" <pvang@sci.kun.nl>
Date: Wed, 20 Sep 2000 10:05:42 +0200
Subject: Spectrum wanted.
Organization: KUN OC MS

Hello MSrs,

could anyone of you mail a picture of PFK recorded using Ammonia-CI.
I'm trying to do so on a old VG7070 where it wasn't performed on yet.
I followed the manual but encounter quite a lot of background or ionising
differences??
I rinsed the tubing and evaporated it. Tubing is flushed with ammonia.
So if anyone can show me a clear/clean picture it would be appreciated very
much.

best regards

Peter

mailto:pvang@sci.kun.nl







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From: cody@jeol.com (Chip Cody)
Subject: Re: Spectrum wanted.
Date: Fri, 22 Sep 2000 16:42:12 GMT
Organization: JEOL USA, Inc.

PFK is not readily ionized by ammonia CI.  Ammonia CI requires that the
analyte have a higher proton affinity than the reagent ions or a
polarity high enough to permit ammonium attachment.  PFK is neither
basic or polar.  However, I was recently reminded that a mixture of
methane and ammonia can provide excellent results with PFK.  The
reference is:

Pergantis, S. A.; Emond, C. A.; Madilao, L. L.; Eigendorf, G. K., Org.
Mass Spectrom. 29 (1994), 439-444.

This requires a little plumbing, but it is an excellent solution to the
need for an internal reference for ammonia CI.

	Chip Cody (JEOL)



-- 
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  |_                                         Robert B. Cody, Ph.D
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  |__                                        Mass Spectrometry
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From: "chris.lee" <chris.lee@infonie.fr>
Date: Tue, 26 Sep 2000 21:35:17 +0200
Subject: Re: Spectrum wanted.
Organization: Infonie

PFK - I assume you mean perfluorokerosine - has a very low proton affinity,
so it won't ionise in ammonia. All you see are the impurities.

If it did ionise under these conditions, there would no longer be a problem
in calibrating the mass axis of TOF machines with API sources.

Regards

******************************************************************************
From: "Little, James L " <jllittle@eastman.com>
Date: Sat, 23 Sep 2000 15:55:53 -0400
Subject: Accurate Mass Data with Ammonia as Reference
Organization: *

I have had some success using the following method:

A General Method for Accurate Mass Measurement of Ammonia CI Spectra Using
PFK Internal Reference

By carefully optimizing pressure, PFK can be used for ammonia CI work.
W. F. Haddon, USDA and Duke University, p1396, 
Proceedings of the 36th ASMS Conference on Mass Spectrometry and Allied
Topics
San Francisco (1988). 

James L. Little
Bldg 150
Eastman Chemical Company
Kingsport, TN 37662-5150
Tel 423-229-8685
FAX 423-229-4558

"A Little Mass Spectrometry and Sailing":
http://users.chartertn.net/slittle
Sailing Club (Intranet) Web Page: http://eastmanweb/fp39/
Adobe Acrobat (Intranet) Software:
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