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From: "David Sparkman" <davidsparkman@home.com>
Date: Tue, 03 Aug 1999 02:50:51 GMT
Subject: MS of Proteins from 2D Gels
Organization: @Home Network

I am looking for information about the mass spectrometry of proteins
isolated using 2D Gels.  I would also like to know what ions may contaminate
the mass spectra of proteins resulting from the media and/or the stain.

Regards;
O. David Sparkman
Consultant-At-Large

ods@compuserve.com
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From: david_stranz@MassSpec.com (David Stranz)
Date: Tue, 03 Aug 1999 18:36:56 GMT
Subject: Re: MS of Proteins from 2D Gels
Organization: Sierra Analytics, LLC


Protana's web site has a large list of typical contaminants found in
protein and protein digest samples.  Matthias Mann spoke of this at
ASMS.  I don't have the exact URL at hand, but www.protana.com will
get you started.

David Stranz
(Not so large consultant)

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From: Morten Nedertoft <mmn@pr-group.ou.dk>
Date: Fri, 06 Aug 1999 17:15:31 +0200
Subject: Re: MS of Proteins from 2D Gels
Organization: UNI-C

try http://www.pil.sdu.dk/

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From: post@stensballe.com (Allan Stensballe)
Date: Tue, 17 Aug 1999 00:57:00 GMT
Subject: Re: MS of Proteins from 2D Gels
Organization: get2net Internet Kunde

Not being a consultant at all, I still have a couple of hints that might help. 

There are valuable information to find in this article (a little old/basic maybe,
but still....- The book is quite nice): Sample preparation methods for mass
spectrometric peptide mapping directly from 2-DE gels. Jensen ON, Wilm M,
Shevchenko A, Mann M Methods Mol Biol 1999;112:513-30.

If you are aware of problems of keratins and use the purest chemicals available at
all stages of the sample preparation (incl. 18Mohm water) there should not be major
problems with MALDI/nESI mass-spec analysis even in the very low fmol
concentrations.

If you _do_ have contaminants or very low sample contrations, a technique that have
helped me a lot is latest presented by a handy swede: Gobom J, et al. Sample
purification and preparation technique based on nano-scale reversed-phase columns
for the sensitive analysis of complex peptide mixtures by matrix-assisted laser
desorption/ionization mass spectrometry. J Mass Spectrom. 1999 Feb;34(2):105-16.

Allan
Member of Roepstorff P. Group (& Mann M.)
University of Southern Denmark..
http://www.protein.ou.dk/