****************************************************************************** From: Gunter Kuhnle Date: Thu, 12 Feb 1998 09:27:31 +0100 Subject: Carotenoids with MALDI or ESI Organization: Uni Leipzig I would like to get mass spectra of carotenoids with MALDI or ESI. Unfortunately, there is no much information in literature about this. Does anyone has experience with this and could give me some information (solvent, matrix, etc)? Thanks, Gunter Kuhnle ****************************************************************************** From: gt6801a@prism.gatech.edu (Wilson Z. Shou) Date: 12 Feb 1998 09:36:46 -0500 Subject: Re: Carotenoids with MALDI or ESI Organization: Georgia Institute of Technology I remember seeing a review paper on Anal. Chem. about using LC/MS (including stop-flow FAB, particle beam, ESI and APCI) to analyze carotenoids. I'm not sure about the exact volume and page number, but it should be around late 95 to 96 and the paper was in the cover of that issue. I suspect the ability of MALDI to do this though. Hope this helps. ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Carotenoids with MALDI or ESI Date: Thu, 12 Feb 1998 15:18:23 GMT Organization: JEOL USA, Inc. I haven't tried MALDI or ESI for carotenoids, but the absence of polar or basic functional groups and the relatively low molecular weight suggests that you might do better to use APCI instead of ESI. Also, these compounds are soluble in non-polar solvent such as benzene and chloroform, which are not favored solvents for ESI. You might see if acetonitrile will work. -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: gal@unice.fr (J.-F. Gal) Date: Sat, 14 Feb 1998 10:56:55 +0000 Subject: Carotenoids with MALDI or ESI Organization: * Hi Gunther, There is a recent review in Anal Chem, 1996, 68, 299A-304A, "Carotenoid analysis using LC/MS" by R.B. van Breemen, with a few references to ESI (the cover is very colorful). The same author has written on APCI of carotenoids : JMS, 1996, 31, 975-981. You may check with the author (Univ of Chicago, College of Pharmacy) if he knows about MALDI works. Best wishes. ------------------------------------- Prof. J.-F. GAL Groupe FT-ICR*GRECFO-Chim. Phys. Org. Fac. des Sciences*Parc Valrose Univ. de Nice-Sophia Antipolis 06108 NICE Cedex 2*FRANCE Tel : (33-4) 04 92 07 61 10 Fax : (33-4) 04 92 07 61 11 E-mail : gal@unice.fr ------------------------------------- ****************************************************************************** From: "rat" Date: Mon, 16 Feb 1998 15:08:13 +1100 Subject: Re: Carotenoids with MALDI or ESI Organization: Monash Uni Hi Gunter, I've run some carotenoids in APCI, works quite well, tried the same with ESI with less success. Stuart Thomson Facility manager - Mass Spectrometry Victorian College of Pharmacy Monash University Parkville Victoria Australia http://www.vcp.monash.edu.au/chemistry/mspec.html ****************************************************************************** From: "Richard B. van Breemen" Subject: Re: Carotenoids with MALDI or ESI Organization: * Please read my review articles or original research papers on electrospray LC-MS of carotenoids as follows: R. B. van Breemen, "Liquid Chromatography/Mass Spectrometry of Carotenoids." (1997) Pure & Appl. Chem., 69, 2061-2066. R. B. van Breemen, "Innovations in Carotenoid Analysis Using LC/MS." Anal. Chem., (1996) 68, 299A-304A. R. B. van Breemen, "Electrospray LC-MS of Carotenoids." Anal. Chem., (1995) 67, 2994-2009. or APCI LC-MS of Carotenoids: R. B. van Breemen, C. R. Huang, Y. Tan, L. C. Sander, A. B. Schilling, "Liquid Chromatography/Mass Spectrometry of Carotenoids Using Atmospheric Pressure Chemical Ionization." J. Mass Spectrom., (1996) 31, 975-981. or these papers on MALDI of carotenoids: R. Kaufman, T. Wingerath, D. Kirsch, W. Stahl, H. Sies, "Analysis of Carotenoids and Carotenol Fatty Acid Esters by Matrix-Assisted Laser Desorption Ionization (MALDI) and MALDI-post source decay Mass Spectrometry." Anal. Biochem. (1996) 238, 117-128. T. Wingerath, W. Stahl, D. Kirsch, R. Kaufmann, H. Sies, "Fruit Juice Carotenol Fatty Acid Esters and Carotenoids as Identified by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry." J. Agric. Food Chem. (1996) 44, 2006-2013. There really is plenty in the literature concerning mass spectrometry of carotenoids. Richard B. van Breemen Department of Medicinal Chemistry & Pharmacognosy University of Illinois at Chicago College of Pharmacy 833 S. Wood Street Chicago, IL 60612-7231 ****************************************************************************** From: Eric_Block@waters.com Date: Tue, 17 Feb 1998 10:13:51 -0400 Subject: Re: Carotenoids with MALDI or ESI Organization: * I have run carotenoids by particle-beam interface-if you're not sample-limited (i.e. you can put a few ug on-column) you get very nice library-searchable EI spectra. I have also done some work with retinol degradants. As Dr. Cody has correctly surmised, APCI (positive) worked best, even though the mobile phase was 100 organic phase.