******************************************************************************
From: Ivan <ivanyip@netvigator.com>
Date: Mon, 14 Apr 1997 17:59:29 +0800
Subject: MSMS of carbohydrates and metal ions
Organization: Netvigator

Can anyone tell me the fragmentation pathway of sugars and effects on
the fragmentation of sugars in tandem MS in the presence of alkali
metals or transition metals? Thanks for your answers!
-- 
mailto:ivanyip@netvigator.com or
mailto:ivanyip@usa.net



******************************************************************************
From: Achim Treumann <atreuman@fujita-hu.ac.jp>
Date: Fri, 18 Apr 1997 11:04:22 +0900
Subject: Re: MSMS of carbohydrates and metal ions
Organization: Fujita Health University


Dear Ivan, 

In the following I assume that you are interested in CID fragmentation
of carbohydrates in electrospray ionisation mass spectrometry.

Vern Reinhold has done some impressive work on the analysis of
methyl-derivatised oligosaccharides in the presence of sodium. 

Linsley KB, 1994           [See Related Articles] 
      Applications of electrospray mass spectrometry to erythropoietin
N- and O-linked glycans. 
      Anal. Biochem. 219, 207-217 (1994) 

 Reinhold VN, 1996           [See Related Articles] 
      Carbohydrate sequence analysis by electrospray ionization-mass
spectrometry. 
      Meth. Enzymol. 271, 377-402 (1996) (no abstract available)

Stroud MR, 1996           [See Related Articles] 
      Monosialogangliosides of human myelogenous leukemia HL60 cells and
      normal human leukocytes.
      2. Characterization of E-selectin binding fractions, and structural
      requirements for physiological binding to E-selectin. 
      Biochemistry 35, 770-778 (1996) 

These are a few references that should get you going. 

Another reference that might be interesting for you would be:
	(1)     Kohler, M.; Leary, J. A. (1995), Analytical Chemistry 
      673501-3508.
      LC/MS/MS of carbohydrates with postcolumn addition of metal
      chlorides using a triaxial electrospray probe.

although I have not tried this myself. 

I will crosspost my answer together with your request to
sci.techniques.mass-spec, you might get more replies from there. 

Good luck, Achim


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. Achim Treumann                 Tel. 81-562-93-9381
Division of Biopolymer Science     Fax  81-562-93-8832
Inst. Comprehensive Med. Sci.
Fujita Health University
Toyoake, Aichi 470-11, Japan


******************************************************************************