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From: "Dr. S. K. Singhal" <ksinghal@mni.uwo.ca>
Subject: Question: Tandem MS of amino acids?
Date: 14 Jul 1995 18:47:50 GMT
Organization: The University of Western Ontario

I am working on solving the structure of an unknown molecule using 
daughter ion analysis by ms/ms. I am using a Sciex API-III triple 
quadruple instrument with an ionspray interface, usually with an LC.

I have one fragmentation pattern that I think may be consistent with 
glutamic acid.  Does anyone know where I might find information on 
fragmentation of amino acids using tandem ms techniques? 

Please post your reply in this newsgroup or by E-mail to:

   ksinghal@mni.uwo.ca

Thanks!

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From: dhchace@acpub.duke.edu (Donald H. Chace)
Subject: Re: Question: Tandem MS of amino acids?
Date: 15 Jul 1995 02:10:58 GMT
Organization: Duke University Medical Center


I have been working on the tandem mass spectrometry of amino acids
for the past 3-4 years as part of our newborn screening R&D.  A fragment
that is observed arises as a result of a neutral loss of formic acid. 
Generally, much of our framentation data on amino acids are form butyl esters 
derivatives of amino acids in which a fragment is produced from the loss of butyl
formate.
These are neutral losses.



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From: dhchace@acpub.duke.edu (Donald H. Chace)
Subject: Re: Question: Tandem MS of amino acids?
Date: 15 Jul 1995 02:16:25 GMT
Organization: Duke University Medical Center

You should expect a neutral loss of COOH. I have fragmentation data from numerous amino acids 
(primarily butyl esters) that may be helpful to you.