****************************************************************************** From: David Bostwick Date: Sun, 2 Jan 2005 20:29:57 -0500 Subject: Forged newsgroup postings Organization: * If you're just starting to read the newsgroup again after vacation, you'll see two articles that are obvious forgeries. If you've been reading regularly, you've already seen them. Similar articles were forged in several other newsgroups at the same time, and there was a similar batch about a month ago. The articles were an attack on a newsgroup about Usenet abuse. The followup line was set to that group with the intention of flooding it with messages. Unfortunately, Usenet was set up in a time with less of the purposeful harm that's becoming more common, and the security for approving articles is far from perfect. I'm sure that it's evident that the articles are forgeries, but I wanted to make it clear that we did not approve them, and they were not sent by the person whose address appears in the header. David Bostwick -- Mass Spectrometry Laboratory Georgia Institute of Technology Atlanta, GA 30332-0400 Tel: 404-385-4250 FAX: 404-894-4061 ****************************************************************************** From: cody@jeol.com Date: 3 Jan 2005 11:04:21 -0800 Subject: Re: Ionization Suppression Under Air Peak (Agilent 5973 MSD)?? Organization: * Don wrote: } "Rich Trubey" wrote in message } news:cqf21g$lnr$1@news-int2.gatech.edu... } } I am trying to detect various light gases using a DB-1 column directed } } to an MSD. For target analytes that elute under the air peak (my } } injections contain small amount of air), has anyone ever seen } } suppression of analyte ionization due to simultaneous ionization of air } } gases? Target analytes are at ppm levels. Injections are 1 mL volume } } (sometimes split, sometimes s/less) via a GasTight syringe. } } } } I know that typically a PLOT column would be better but that is not } } necessarily an option for me. } } How long is your column? You will have a hard time separating permanent gases with a 30 m column. I find that a 60 meter column is almost the minimum length for working with GC/MS of light gases if you are using a common phase like DB-1. Chip Cody JEOL USA, Inc. ****************************************************************************** From: Mike Sherrell Date: Wed, 5 Jan 2005 08:36:01 -0800 Subject: Mass specs, MALDIs available Organization: * Mass specs, MALDIs available from Grizzly Analytical 707 887 2919/mike@grizzlyanalytical.com (For text string search in Outlook Express, use ctrl+shift+F; in Outlook use F4) **LC/MS & MS/MS: Q-Star Pulsar i: price in negotiations. 2002 system. Accepts oMALDI source. Call/email if interested. Q-Star Pulsar: $170,000. Not the "i". Recently pmd. + $16K/factory install. Q-Star Pulsar XL: Call/email to discuss price. Hybrid Q-TOF. Sciex API 4000: $270,000. Running in lab now; can be tested. API 3000 upgrade: $38,500. Increases sensitivity and (S/N) Ratio at high flow rates to ~ that of an API 4000. Install incl. Sciex API 2000: $95,000 installed and warranteed. Sciex API 2000: $120,000: Incl. EPQ3 upgrade to ~ API 3000 sensitivity, install & Warranty. Sciex API 2000 upgrade: $25,000. 4x sensitivity increase; incl. install, warr. Sciex API 365: $65,000. NT, turboionspray. Installed, warranteed. Sciex 365 w/ EP10+: $149,000. Custom upgrade; more sensitive than API 3000. Sciex API 365 upgrade: $109,000. 10x+ sensitivity upgrade; near-equal to 4000. Sciex API 150EX: $48,000. MCA upgraded to EX; identical performance. MAC; NT + $10,000. Incl. install. Sciex NT workstation: $2,500. Use to upgrade Macs on 150, 365, 2000, 3000. Sciex API III+: $25,000. Triple quad: ES, APCI; +$24K/intall w/ 1 yr. warr. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $20,000/installation and 1 year service contract. Sciex Turboionspray source (aka ESI) for API 150, 365 or 3000: $6,250. Sciex MicroIon spray source: $7,900. For API 150, 300 or Q-Star. Very low flow. PE-ABI Mariner: $45,000. Price includes factory install. Agilent 1100 MSD Trap. Call to discuss price. 2001 model. Agilent 1100 MSD: $various. All Models (A - D) with varying sources (ESI, APCI, APPI). Most any configuration. Can include install, 90-day warranty and training. Agilent 1100 VL LC/MSD: offers considered. 2004 system. Finnigan Quantum AM: $320,000. 2004 system. Incl. install, 1 yr. svc. Finnigan Quantum: $160,000. ESI only (APCI available); factory refurbed; guaranteed installable. Install/warranty avail. Finnigan Discovery Max: $179,000. ew; specs comparable to Sciex API 3000. Incl. install, 1 year svc. Finnigan Deca XP+: $150,500. 2002; pristine; installed and calibrated but never used. Includes factory install, guar. eligible for svc. Finnigan Deca XP: $135,000. 30000 model. Includes install and 1 year svc. Factory upgrade to XP + for addt'l $14,000. Finnigan LCQ DECA: $67,500. ESI, Xcalibur 1.2, install, warranty incl. Finnigan LCQ DUO: $75,000 installed. Finnigan LCQ Classic: $58,500. ESI, installation, 90-day warr. LCQ Classic ESI source: $7,500. New/unused ESI source. Finnigan Navigator: $29,500. Single quad. Installed, 30-day warr. Finnigan TSQ 7000: $50,000. ES, Xcalibur software, installation and 30-day warranty. Workhorse triple quad. Finnigan TSQ 7000 GC/MS: $65,000. GC and LC-capable; Xcalibur. Install, warr. avail. Finnigan SSQ 7000: $45,000. ES, APCI; Excalibur 1.0; API 1 source; install incl. Finnigan TSQ 700: $30,000. Electrospray, APCI. Install included. Finnigan SSQ 710: $25,000. Electrospray, APCI, API 1, Alpha workstation, install included. Finnigan Mat ITS40W. Call to discuss price. With Varian 3400 GC + A200s Auto Sampler. Bruker Esquire: $85,000. Ion trap. Incl. install, guaranteed. Hitachi M 8000: $82,000. Ion trap. 1999; excellent condition; incl. LC. Micromass Quattro micro API: $150,000; Complete 2000. On factory service contract when deinstalled. Micromass LCT API-oaTOF MS: $160,000. Sold new for $260,000 in July 2000. Includes Waters HPLC. Micromass Quattro LCZ: $131,500. Includes installation, warranty. Micromass Quattro IIZ: $149,000. Z-Spray. Includes install, warranty. Micromass Quattro Ultima: $125,000. VE. Z-Spray, APCI-Z, new MassLynx workstation. Micromass Quattro II: $150-200K. Price depends on whether you want installation, GC and/or HPLC. Micromass Q-Tof II: $185,000. Hybrid Quadrupole. Installed, eligible for service contract. MM/Waters ZMD 4000: $49,000. ESI, APCI. +$10K/factory install. Micromass ZABSpec Ultima OA TOF: $89,000. Mag sector/TOF hybrid. 1997 model. Good working order. Micromass Autospec M: $179,000. Mag sector/TOF MS/MS. Incl. FPD, 5 sources, guar. eligible for svc. ($700K new) Shimadzu 2010: offers considered. 2001 system; single quad; ESI/APCI. Fisons VG 2000. <$100,000. Fisons VG Trio: $25,000. LC + GC: 3000 amu; thermospray, EI/CI, HP 5890 included. Install, license & 90-day warr. + $14,500. VG Trio 2: $7,500. Electrospray; complete; parts or fixer-upper unit. Nermag R10c. <$10,000. Like new; make offer. Finnigan MAT 90: $16,000. All parts intact, plus spares included. MM Autospec: call if interested. Mag Sector. TOF, MSMS, ESI, MALDI, EI/CI. MM Autospec S: $60,000. European install included; available in US. MM Autospec V: $70,000. European install included; available in US. Mass spec sample introduction systems listed under liquid handlers, below. *Service and service contracts available for PESciex API 3000, 365 and III+. **MALDI-TOFs: Voyager DE: $45,000. Installed, guaranteed. Voyager DE STR: $138,500. Includes install; guaranteed eligible for svc contract. Voyager DE Pro: $109,000. Incl. factory install, certification. Voyager DE RP: $67,000. Extensively refurbished. Voyager RP: $44,000. Incl. install; service contracts avail. Will QC peptides; continuous extraction. Voyager Elite XL: offers considered. incl. Vestec Multi-gauge, Advantec fraction collector Voyager MALDI lasers: rebuilt; full warrantee; ~2/3 price of new; install available. Call for info. Micromass M@LDI LR: $79,000. 2002 linear + reflection benchtop; determine intact protein mass and mPMF. Install included. Mariner ESI-TOF: $45,000. Installed/guar. ok for factory service. MicroMass TOF 2e-Spec: call/email to discuss price. 1999; many options; good working order. MicroMass Q-TOF API-US: call/email to discuss price. 2002; includes CapLC. MicroMass Q-Tof Ultima Global: call/email to discuss price. Complete 2002 system. Micromass Reflectron: $110,000. Incl. MassPrep enclosed robotic sampler system. + $30,500/install. Micromass Q-Tof 2: $90,000. 2000 model; Nanoflow-Z CapElectrophoresis electrospray. Guaranteed installable. Micromass Q-Tof 1: $155,000. + $41K/install and license. Incl. CapLC, many upgrades and extras. MicroMass LC-TOF: $90,000. API-LC/Orth. 2000 model. Manufacturer-certified. Micromass LCT. ~$155,000. API-TOF. Includes HPLC. New July 2000. Sequenom System: $215,000. 2001 model. Bruker BiFlex III, Oracle software, SpectroCheck, Reader and Jet. Installation incl. Sequenom MassARRAY: offers considered. 7K system (2002), for genotyping. Current software. Bruker MicrOTOF: $195,000. 2004 model; lease default. Incl. install, warranty. Bruker Ultraflex: call/email to discuss price. Working perfectly in lab Bruker Reflex IV: $207,500. 2001 model; list $300K. Incl. ion source, TOF analyzer, detector, two NT processing stations. Bruker OmniFlex: $69,750. 2000 model; all hardware upgrades; factory install, warranty included. Bruker Reflex III: $89,000. 1999 model; includes chiller, standalone AS-90. Bruker Biflex III: various systems available; call or email to discuss Bruker Proflex III: offers considered. Excellent condition. LaserTec II: $75,000. By PerSeptive. 5 yrs. old; excellent condition. Thermo-Finnigan Dynamo: $55,000. Linear DE benchtop system; 2 yrs. old; pos/neg. Price includes ship, install, train, 90-day warr. Finnigan LaserMAT 2000: $25,000. Includes ship, install, 90-day parts warr. Kratos Kompact III: offers considered; call or email if interested. SRI custom design: $100,000. Ideal for SNP determination. Asking price. 384 samples/20 min. Can be tested. Finnigan MAT Vision 2000: $80,000. Reflectron. Includes install, 1 year warranty. VG Tof-Spec: $6,000. Or best offer. For parts; new laser card and other new boards. Waters MALDI prep device. Offers considered. Used only once. Includes plates and kits. *Also available: service/contracts on Voyager DE, DE RP and PRO. **GC/Other MS: Micromass VG70 SE: $45,000. Hi resolution GC/MS MSI Autoconcept: $380,000. New; multiramp temp; Agilent GC and Agilent or CTC autosampler. Includes install, 1 yr. warranty. Thermoquest GCQ: $30,000. GC/MS/MS; EI/CI; rebuilt; 90-day warranty. Agilent 5973/6890: $65,000. EI/CI/NCI; one year warranty. HP 5890 II/5970B: Offers considered. SmartCard II, software incl. HP 5989 MS engine: $28,500. 2000 amu mass range, pos/neg CI, APCI. Finnigan Trace 2000: offers considered. 1998. EI/CI, autosampler, NIST library, Xcalibur Finnigan Magnum GC/MS Ion trap: $7,500. Incl. GC. Finnigan MAT GCQ: $23,000. GC/MS/MS. EI/CI. Finnigan MAT SOLA: $50,000. Asking price. 8 yrs. old; incl. GF, hydrides gen. Finnigan T-30 Newstar. Call to discuss price. FT-MS. Was $1.4M in 1997. Price negotiable. JOEL HX 110. Call to discuss price. Tandem Mass spec. Micromass Platform ICP-MS: offers considered. 2001 model; 3rd collision gas option. Regards, Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: edpappas@yahoo.com Date: 5 Jan 2005 11:05:45 -0800 Subject: Retention Index Macro? Organization: * Hello all, First time user of sci.techniques.mass-spec ... anyways, I was wondering if anyone knows if a macro is available to compute retention index values for each peak detected. I'm using GC/MS Chemstation (B build with Enviroquant software). Been searching high and low with no luck! Thanks.... Ed Pappas ****************************************************************************** From: ChemUserWorld Date: Wed, 5 Jan 2005 12:47:04 -0800 Subject: Re: Retention Index Macro? Organization: * Ed, You may want to post this question to the discussion group at http://www.ChemUserWorld.com. This site specializes in ChemStation issues. Regards Marcom Marcom no @ spam ChemUserWorld.com wrote in message news:crhe9d$r1l$1@news-int.gatech.edu... } Hello all, } First time user of sci.techniques.mass-spec ... anyways, I was } wondering if anyone knows if a macro is available to compute retention } index values for each peak detected. I'm using GC/MS Chemstation (B } build with Enviroquant software). Been searching high and low with no } luck! Thanks.... Ed Pappas } } ****************************************************************************** From: Mike Date: Wed, 5 Jan 2005 15:32:27 -0800 Subject: Bruker Daltonics (Canada) Employment Opportunity Organization: * Bruker Daltonics (Canada) Ltd. has an immediate opening for an Electronic Service Engineer based in the Milton, Ontario office (Toronto Area). If you are, or know of a qualified person who would be interested in this position, we ask you to submit a resume in complete confidence to the contact point indicated. ELECTRONIC SERVICE ENGINEER BSc. or MSc. Electrical Engineering The principal duties involved will consist of installation and service of our Mass Spectrometers, and training our customers from the perspective of a service background. Working in collaboration with our Mass Spectrometry specialist, you will participate in the installation and commissioning of cutting edge instrumentation in some of Canada’s most prominent research labs and Universities. It is desirable for candidates to have minimum five years industrial troubleshooting experience, preferable with laboratory instrumentation. Occasional travel across Canada and to the U.S. required. Experience/knowledge of Mass Spec systems will be a definite asset, but not mandatory. The ideal candidate will have experience in the following fields: * RF and Digital Electronics troubleshooting * High Vacuum Systems including turbo molecular pumps * High Voltage power supplies * Strong computer skills including networking * Biochemistry or Life Science Research Lab environment Applicants’ personal profiles will contain most of the following: * A University or College degree in Electrical Engineering or Electronics * Experience in complex electronic systems maintenance and troubleshooting. * Evidence of being self-sufficient and independent in your approach to your work. * You have good communication skills and are able to interact comfortably with our customers, many of who are leading scientists in their fields. Bruker Daltonics Ltd. is an equal opportunity employer and is strongly committed to the principles and practice of Ontario gender equity legislation. Please reply in writing only (fax or mail) to: Thomas Buser Service Manager Bruker BioSpin Ltd. 555 Steeles Avenue East Milton, Ontario L9T 1Y6 Fax: (905) 876-4421 Applications from agents will be dismissed out of hand and are likely to result in the disqualification of an otherwise suitable candidate. ****************************************************************************** From: Jeff Gambera Date: Thu, 06 Jan 2005 01:35:07 GMT Subject: Re: HP Engine Organization: * Concerning the diffusion pumps-, assuming you have sufficient fluid in them, to quickly check if they are at the proper temperature you can lick your finger and lightly touch the bottom side of the pumps, it should sizzle. also the diff pumps have both an over temp cutout switch and an low temp cutout switch. If the water is too cold the pumps will not turn on. To check for gross leaks turn off the roughing pumps and watch the rise of the pressure. It should take a while if there are no gross leaks. Another thing to try is to remove all interface assemblys on both sides of the manifold and replace with rubber stoppers. ( to eliminate possibility of hairline cracks in the transfer line welds). If all the above checks out OK then you have a vacuum gauge problem. The ion gauge will only become enabled once the pressure falls into the high 10-3 range. otherwise the gauge filiment will not come on or will go out instantly. Good Luck Bill Heriot NIAID Corp. "Beverley Earl" wrote in message news:cpl55k$4s9$1@news-int2.gatech.edu... } } In September I had a problem where I thought the diffusion pumps on the } HP5989A were not coming on. The instrument had been off for some time and } when I started it up the pressure on the manifold read 4x10-2. I blocked } off the column and the pressure reduced to 2x10-2 still a significant } leak. Since then I have: } } - replaced the manifold window seal } } - blocked off both sides (separately) of the analyser e.g. interface and } probe port and replaced the O ring at each side. } } - checked the oil level and replaced the O rings on the diffusion pumps } } - added ptfe tape to the vent plug at the back which lets the air in when } venting } } - tightened the nuts on the CI and other valves attached to the interface } } - checked the level of calibration liquid in the calibration valve. } } - checked that the rough pump lever is closed properly } } All were done separately and had no effect on the pressure as it remains } at 2x10-2 with the column blocked off and the problem is that the more I } do the more likely it is that I am introducing another problem! } } Initially I thought the diffusion pumps were not coming on as the } pressure remains constant when they are turned off to vent the instrument } i.e. 2x10-2. The pressure does not change when the pumps are switched off } to cool but they do heat up. This implies to me that the rough pumps are } achieving the pressure without the diffusion pumps. The back diffusion } pump oil was slightly more yellow and the O rings more difficult to } remove but there wasn't any particulate matter and the oil levels were } ok. } } With the diffusion pumps off and the rough pumps on I put up the baffles } which are manually operated but the pressure did not change if this helps } at all? } } We do not have a maintenance contract and I need to exhaust every option } before we can consider paying for an engineer. } } Since then I realised that they are heating up and any further advice you } can offer would be welcome. } } Thanks } } Beverley } } } ****************************************************************************** From: Phil Date: Thu, 6 Jan 2005 16:55:40 +0100 Subject: Re: Retention Index Macro? Organization: * Yeap! Used to write a package for this...Was some time ago!! Just have to dig into my oldies to see if I still have the compatible version for the B version. Let me check and I'll get back to you... Phil ****************************************************************************** From: THOMVS@inel.gov Date: Thu, 6 Jan 2005 17:06:50 -0700 Subject: contamination peaks Organization: * I would like to post a question to your newgroup; however, I have found out that my company firewall prevents me from accessing it directly. I was hoping that the question could be posted and the replies routed to me through email. If this would be possible, please let me know. Thanks. The question is given below. I have been having recurrent contamination peaks that occur in my calibration standard. The peaks of concern occur at m/z 101 and 149. There are also isotopic peaks at 102, 103 and 150 and 151, respectively. The x+1 peak is approximately 10% and the x+2 peak is approximately 1% the magnitude of the peaks at 101 and 149. My calibrant is glu-fibrinopeptide B in a solution of 30% methanol and 1% formic acid in water. I am introducing the calibrant using an electrospray source into an Applied Biosystem QSTAR XL instrument. Some testing by me has indicated that the source of the contamination may be the methanol, but some other tests contradict that. I have also seen these peaks in some of my samples that use methanol as the solvent. Has anyone encountered these peaks before? If so, what are they and how can you reduce or eliminate them? Vicki Thompson Biotechnology Dept. Phone: (208) 526-8833 FAX: (208) 526-0828 thomvs@inel.gov ****************************************************************************** From: Graeme Robertson Date: Fri, 7 Jan 2005 10:14:38 -0000 Subject: Re: contamination peaks Organization: * Hi Vicki, 149 peak is typically plastciser related peak from esters of phthalic acid see http://www.lipidlibrary.co.uk/ms/arch_xyz/xyz_fa/add004.gif for an example. Probably from your solvent methanol. You may not have exactly the same plasticiser but they are all phthalic acid derived and give the signature 149 peak. It is difficult to eliminate these from organic solvents completely. Use glassware for handling your samples when at all possible. Plastics and organic solvents don't do well together. If they dont interfere with your analysis and are present at low levels I would ignore them. Try sourcing some chromatography analytical solvents also rather than routine lab solvents. Solvents for pesticide residues or similar etc GOOD LUCK. wrote in message news:crksr8$6q4$1@news-int.gatech.edu... } } } } I would like to post a question to your newgroup; however, I have found } out that my company firewall prevents me from accessing it directly. I } was hoping that the question could be posted and the replies routed to me } through email. If this would be possible, please let me know. Thanks. The } question is given below. } } I have been having recurrent contamination peaks that occur in my } calibration standard. The peaks of concern occur at m/z 101 and 149. } There are also isotopic peaks at 102, 103 and 150 and 151, respectively. } The x+1 peak is approximately 10% and the x+2 peak is approximately 1% } the magnitude of the peaks at 101 and 149. My calibrant is } glu-fibrinopeptide B in a solution of 30% methanol and 1% formic acid in } water. I am introducing the calibrant using an electrospray source into } an Applied Biosystem QSTAR XL instrument. Some testing by me has } indicated that the source of the contamination may be the methanol, but } some other tests contradict that. I have also seen these peaks in some of } my samples that use methanol as the solvent. Has anyone encountered these } peaks before? If so, what are they and how can you reduce or eliminate } them? } } } Vicki Thompson } Biotechnology Dept. } Phone: (208) 526-8833 } FAX: (208) 526-0828 } thomvs@inel.gov } ****************************************************************************** From: David Bostwick Date: Fri, 07 Jan 2005 16:32:53 GMT Subject: Re: contamination peaks Organization: * In article , Graeme Robertson wrote: }Hi Vicki, }149 peak is typically plastciser related peak from esters of phthalic acid }see http://www.lipidlibrary.co.uk/ms/arch_xyz/xyz_fa/add004.gif for an }example. Probably from your solvent methanol. You may not have exactly the }same plasticiser but they are all phthalic acid derived and give the }signature 149 peak. It is difficult to eliminate these from organic solvents }completely. Use glassware for handling your samples when at all possible. }Plastics and organic solvents don't do well together. If they dont interfere }with your analysis and are present at low levels I would ignore them. Try }sourcing some chromatography analytical solvents also rather than routine }lab solvents. Solvents for pesticide residues or similar etc GOOD LUCK. } The 149 ion (as well as 167 and 279) shows up in the EI spectrum of dioctyl phthalate. In ESI, you should see the [M+H]+ at 391. Other phthalates will show related ions. ****************************************************************************** From: David Sparkman Date: Fri, 7 Jan 2005 07:46:59 -0800 Subject: Re: contamination peaks Organization: * Graeme, I believe you are talking about electron ionization and Vicki is doing electrospray. I don't think a peak in a mass spectrum at m/z 149 produced by ESI is due to a phthalate. In a spectrum produced by EI, yes. Vicki, are you detecting negative or positive ions? Is the peak at m/z 149 a possible MH+ or [M - H]-? Regards; David -- O. David Sparkman Consultant-At-Large ods no @ spam compuserve.com "Graeme Robertson" wrote in message news:crm5tr$rkt$1@news-int2.gatech.edu... } Hi Vicki, } 149 peak is typically plastciser related peak from esters of phthalic acid } see http://www.lipidlibrary.co.uk/ms/arch_xyz/xyz_fa/add004.gif for an } example. Probably from your solvent methanol. You may not have exactly the } same plasticiser but they are all phthalic acid derived and give the } signature 149 peak. It is difficult to eliminate these from organic solvents } completely. Use glassware for handling your samples when at all possible. } Plastics and organic solvents don't do well together. If they dont interfere } with your analysis and are present at low levels I would ignore them. Try } sourcing some chromatography analytical solvents also rather than routine } lab solvents. Solvents for pesticide residues or similar etc GOOD LUCK. } } wrote in message news:crksr8$6q4$1@news-int.gatech.edu... } } } } } } } } I would like to post a question to your newgroup; however, I have found } } out that my company firewall prevents me from accessing it directly. I } } was hoping that the question could be posted and the replies routed to me } } through email. If this would be possible, please let me know. Thanks. The } } question is given below. } } } } I have been having recurrent contamination peaks that occur in my } } calibration standard. The peaks of concern occur at m/z 101 and 149. } } There are also isotopic peaks at 102, 103 and 150 and 151, respectively. } } The x+1 peak is approximately 10% and the x+2 peak is approximately 1% } } the magnitude of the peaks at 101 and 149. My calibrant is } } glu-fibrinopeptide B in a solution of 30% methanol and 1% formic acid in } } water. I am introducing the calibrant using an electrospray source into } } an Applied Biosystem QSTAR XL instrument. Some testing by me has } } indicated that the source of the contamination may be the methanol, but } } some other tests contradict that. I have also seen these peaks in some of } } my samples that use methanol as the solvent. Has anyone encountered these } } peaks before? If so, what are they and how can you reduce or eliminate } } them? } } } } } } Vicki Thompson } } Biotechnology Dept. } } Phone: (208) 526-8833 } } FAX: (208) 526-0828 } } thomvs@inel.gov } } } } } ****************************************************************************** From: Justin Withers Date: Fri, 07 Jan 2005 14:54:33 -0600 Subject: Re: contamination peaks Organization: * Vicki, use the cleanest, most expensive solvents you can lay your hands on, especially for a QSTAR. 149 is definitely a phthalate, even in ESI, Cheers, Justin, AB support (from home) THOMVS@inel.gov wrote: } I would like to post a question to your newgroup; however, I have found } out that my company firewall prevents me from accessing it directly. I } was hoping that the question could be posted and the replies routed to me } through email. If this would be possible, please let me know. Thanks. The } question is given below. } } I have been having recurrent contamination peaks that occur in my } calibration standard. The peaks of concern occur at m/z 101 and 149. } There are also isotopic peaks at 102, 103 and 150 and 151, respectively. } The x+1 peak is approximately 10% and the x+2 peak is approximately 1% } the magnitude of the peaks at 101 and 149. My calibrant is } glu-fibrinopeptide B in a solution of 30% methanol and 1% formic acid in } water. I am introducing the calibrant using an electrospray source into } an Applied Biosystem QSTAR XL instrument. Some testing by me has } indicated that the source of the contamination may be the methanol, but } some other tests contradict that. I have also seen these peaks in some of } my samples that use methanol as the solvent. Has anyone encountered these } peaks before? If so, what are they and how can you reduce or eliminate } them? } } } Vicki Thompson } Biotechnology Dept. } Phone: (208) 526-8833 } FAX: (208) 526-0828 } thomvs@inel.gov } ****************************************************************************** From: Alchemist Date: Sat, 8 Jan 2005 08:04:59 -0500 Subject: Re: Retention Index Macro? Organization: * HP had a package developed at Givaudan. It was called project "Banana". Call your sales person, and they may be able to send you a copy on CD. All files add up to 37MB, so I can't post it here. Good Luck. wrote in message news:crhe9d$r1l$1@news-int.gatech.edu... } Hello all, } First time user of sci.techniques.mass-spec ... anyways, I was } wondering if anyone knows if a macro is available to compute retention } index values for each peak detected. I'm using GC/MS Chemstation (B } build with Enviroquant software). Been searching high and low with no } luck! Thanks.... Ed Pappas } } ****************************************************************************** From: Dave Palmer Date: Sun, 09 Jan 2005 16:36:33 GMT Subject: Re: contamination peaks Organization: * Contamination from phthalate ester plasticizers may not only come from your solvent supply; it could also come from vial caps, etc. Also, it's a pretty good bet that samples that are transferred in ziplock bags will become contaminated with phthalates. (This is a big problem for those of use doing plastics failure analysis. If we recieve a failed part that was sent to us in a plastic bag and we are interested in something happening on the surface of the part, we can't be sure how much of the plasticizer that we see was initially present and how much was picked up in transit. We try to get customers to wrap parts in aluminum foil before sending them, but they don't always do it). Also, the hand soap in our lab's bathroom contains diethyl phthalate, so we have occasionally had problems with diethyl phthalate appearing in samples which people handled after washing their hands. ("But my hands were clean! I washed them!") Dave Palmer ****************************************************************************** From: Eric van der Meulen Date: 10 Jan 2005 14:49:01 -0800 Subject: Re: contamination peaks Organization: * Lots of chemicals, including calibration standards, are shipped with a piece of ducktape wrapped around the cap of the vial, which can also be a source of phtalates. I've also seen a lot of chemists using parafilm for this purpose. I don't know why they do it, but they do..... Eric ****************************************************************************** From: John Bartmess Date: Tue, 11 Jan 2005 08:29:43 -0500 Subject: The Remailing service for this newsgroup Organization: * THOMVS@inel.gov (Vicki Thompson) wrote: }I would like to post a question to your newgroup; however, I have found }but that my company firewall prevents me from accessing it directly. I }was hoping that the question could be posted and the replies routed to me }through email. If this would be possible, please let me know. Thanks. The }question is given below. A reminder to readers: there is a remailing service for this newsgroup, intended in part for folks in this exact situation. To subscribe: + - To subscribe to the STMS remailer, go to: + + ion.chem.utk.edu/mailman/lists/stms and follow the instructions + + + + - To UNSUBSCRIBE, you will need the password that you saved at + + subscription time. Go to the same URL. + + + + If mailings to your address bounce more than twice, I'll remove the + + invalid address from the mailing list, after attempting to contact your + + postmaster. If you have tried to subscribe and get nothing in a week, + + your address is probably bouncing mail. I can't fix that directly; send + + e-mail to me. + John Bartmess ****************************************************************************** From: Mike Wilmarth Date: 11 Jan 2005 10:24:32 -0800 Subject: QUALITY CONTROL SCIENTIST II - Torrance, California Organization: * QUALITY CONTROL SCIENTIST II - Torrance, California I am assisting to recruit a Quality Control Scientist for a biochemical company located in Torrance, California. COMPANY: They are an international, technology-based company specializing in the production of innovative biochemical and pharmaceutical compounds. This position is within the division that has scientific know-how in peptide conjugation, antibody production (against peptides and proteins) for immunoassay, immunohistochemistry and immunocytochemistry, kit formulation and iodination of peptides. The company employs 530 motivated and qualified people worldwide. Here are the details concerning this opportunity: JOB FUNCTION: The Quality Control Scientist II is responsible for raw material, in-process and final product testing. The QC Scientist maintains all required GMP documentation, including lab notebooks; log books, reporting forms etc. The person (s) in this position has more experience than the QC Scientist and thus does more trouble shooting and problem solving than the less experienced staff. ESSENTIAL FUNCTIONS: Some or all of the following activities will be performed by the QC Scientist: Run analytical tests (HPLC, amino acid analysis, gas chromatography, thin layer chromatography, polarimetry, melting point, spectrophotometer, Karl Fischer). Perform stability studies, maintain stability records. Write protocols for, perform and write final reports for analytical methods validation. Prepare buffers/reagents. Develop trending formats, enter and trend data. Maintain records, review and sign records for other QC Scientists . Train other QC Scientists. Release raw materials, label raw material containers with release stickers. ADDITIONAL RESPONSIBILITIES: Special projects as requested for regulatory submissions or customer requirements. EDUCATIONAL REQUIREMENTS: BS degree in science discipline (chemistry, biochemistry, organic chemistry, biology or equivalent) required. EXPERIENCE & QUALIFICATION: 2-5 years experience in analytical chemistry required. Computer experience with Microsoft Word, Excel and Access important. 2-5 years of industry experience in quality control required. GMP experience desirable. SKILLS: Analytical testing. Good computer skills, including word processing (Word) and spreadsheets (Excel). Good technical writing skills. Attention to detail. Good organizational skills. Trouble shooting/problem solving skills APPLY: If you are interested in applying for one of these positions, please forward your Word formatted resume, cover note and salary requirement to resumes@workwondersstaffing.net. Please include the specific position to which you are applying in the subject line of your email. This will ensure your resume is routed to the correct recruiter as quickly as possible. Only qualified candidates will be contacted for interviews. Receipt of your resume will be confirmed if it is sent as an attachment. REFERRALS: If you are not interested in the opportunity for yourself, please consider referring a colleague or friend. We do have a generous referral program. More details are available here: http://www.workwondersstaffing.net/CandidateResources/Referrals.html NOTE: Please note, Work Wonders Staffing is a recruiting firm. No fees are charged to applicants as our fees are paid by our clients. More information is at www.workwondersstaffing.net ****************************************************************************** From: John Bartmess Date: Tue, 11 Jan 2005 13:57:24 -0500 Subject: Corrected URL for Remailer Service Organization: * That last URL for the newsgroup remailer was an old one. Here's the correct one: http://ion.chem.utk.edu/mailman/slistinfo/stms Sorry about that. John Bartmess ****************************************************************************** From: Mike Sherrell Date: Wed, 12 Jan 2005 08:56:06 -0800 Subject: Mass specs, MALDIs available Organization: * Mass specs, MALDIs available from Grizzly Analytical, www.grizzlyanalytical.com: **LC/MS & MS/MS: Q-Star Pulsar i: price in negotiations. 2002 system. Accepts oMALDI source. Call/email if interested. Q-Star Pulsar: $170,000. Not the "i". Recently pmd. + $16K/factory install. Q-Star Pulsar XL: Call/email to discuss price. Hybrid Q-TOF. Sciex API 4000: $270,000. Running in lab now; can be tested. API 3000 upgrade: $38,500. Increases sensitivity and (S/N) Ratio at high flow rates to ~ that of an API 4000. Install incl. Sciex API 2000: $95,000 installed and warranteed. Sciex API 2000: $120,000: Incl. EPQ3 upgrade to ~ API 3000 sensitivity, install & Warranty. Sciex API 2000 upgrade: $25,000. 4x sensitivity increase; incl. install, warr. Sciex API 365: $65,000. NT, turboionspray. Installed, warranteed. Sciex 365 w/ EP10+: $149,000. Custom upgrade; more sensitive than API 3000. Sciex API 365 upgrade: $109,000. 10x+ sensitivity upgrade; near-equal to 4000. Sciex API 150EX: $48,000. MCA upgraded to EX; identical performance. MAC; NT + $10,000. Incl. install. Sciex NT workstation: $2,500. Use to upgrade Macs on 150, 365, 2000, 3000. Sciex API III+: $25,000. Triple quad: ES, APCI; +$24K/intall w/ 1 yr. warr. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $20,000/installation and 1 year service contract. Sciex Turboionspray source (aka ESI) for API 150, 365 or 3000: $6,250. Sciex MicroIon spray source: $7,900. For API 150, 300 or Q-Star. Very low flow. PE-ABI Mariner: $45,000. Price includes factory install. Agilent 1100 MSD Trap. Call to discuss price. 2001 model. Agilent 1100 MSD: $various. All Models (A - D) with varying sources (ESI, APCI, APPI). Most any configuration. Can include install, 90-day warranty and training. Agilent 1100 VL LC/MSD: offers considered. 2004 system. Finnigan Quantum AM: $320,000. 2004 system. Incl. install, 1 yr. svc. Finnigan Quantum: $160,000. ESI only (APCI available); factory refurbed; guaranteed installable. Install/warranty avail. Finnigan Discovery Max: $179,000. ew; specs comparable to Sciex API 3000. Incl. install, 1 year svc. Finnigan Deca XP+: $150,500. 2002; pristine; installed and calibrated but never used. Includes factory install, guar. eligible for svc. Finnigan Deca XP: $135,000. 30000 model. Includes install and 1 year svc. Factory upgrade to XP + for addt'l $14,000. Finnigan LCQ DECA: $67,500. ESI, Xcalibur 1.2, install, warranty incl. Finnigan LCQ DUO: $75,000 installed. Finnigan LCQ Classic: $58,500. ESI, installation, 90-day warr. LCQ Classic ESI source: $7,500. New/unused ESI source. Finnigan Navigator: $29,500. Single quad. Installed, 30-day warr. Finnigan TSQ 7000: $50,000. ES, Xcalibur software, installation and 30-day warranty. Workhorse triple quad. Finnigan TSQ 7000 GC/MS: $65,000. GC and LC-capable; Xcalibur. Install, warr. avail. Finnigan SSQ 7000: $45,000. ES, APCI; Excalibur 1.0; API 1 source; install incl. Finnigan TSQ 700: $30,000. Electrospray, APCI. Install included. Finnigan SSQ 710: $25,000. Electrospray, APCI, API 1, Alpha workstation, install included. Finnigan Mat ITS40W. Call to discuss price. With Varian 3400 GC + A200s Auto Sampler. Bruker Esquire: $85,000. Ion trap. Incl. install, guaranteed. Hitachi M 8000: $82,000. Ion trap. 1999; excellent condition; incl. LC. Micromass Quattro micro API: $150,000; Complete 2000. On factory service contract when deinstalled. Micromass LCT API-oaTOF MS: $160,000. Sold new for $260,000 in July 2000. Includes Waters HPLC. Micromass Quattro LCZ: $131,500. Includes installation, warranty. Micromass Quattro IIZ: $149,000. Z-Spray. Includes install, warranty. Micromass Quattro Ultima: $125,000. VE. Z-Spray, APCI-Z, new MassLynx workstation. Micromass Quattro II: $150-200K. Price depends on whether you want installation, GC and/or HPLC. Micromass Q-Tof II: $185,000. Hybrid Quadrupole. Installed, eligible for service contract. MM/Waters ZMD 4000: $49,000. ESI, APCI. +$10K/factory install. Micromass ZABSpec Ultima OA TOF: $89,000. Mag sector/TOF hybrid. 1997 model. Good working order. Micromass Autospec M: $179,000. Mag sector/TOF MS/MS. Incl. FPD, 5 sources, guar. eligible for svc. ($700K new) Shimadzu 2010: offers considered. 2001 system; single quad; ESI/APCI. Fisons VG 2000. <$100,000. Fisons VG Trio: $25,000. LC + GC: 3000 amu; thermospray, EI/CI, HP 5890 included. Install, license & 90-day warr. + $14,500. VG Trio 2: $7,500. Electrospray; complete; parts or fixer-upper unit. Nermag R10c. <$10,000. Like new; make offer. Finnigan MAT 90: $16,000. All parts intact, plus spares included. MM Autospec: call if interested. Mag Sector. TOF, MSMS, ESI, MALDI, EI/CI. MM Autospec S: $60,000. European install included; available in US. MM Autospec V: $70,000. European install included; available in US. Mass spec sample introduction systems listed under liquid handlers, below. *Service and service contracts available for PESciex API 3000, 365 and III+. **MALDI-TOFs: Voyager DE: $45,000. Installed, guaranteed. Voyager DE STR: $138,500. Includes install; guaranteed eligible for svc contract. Voyager DE Pro: $109,000. Incl. factory install, certification. Voyager DE RP: $67,000. Extensively refurbished. Voyager RP: $44,000. Incl. install; service contracts avail. Will QC peptides; continuous extraction. Voyager Elite XL: offers considered. incl. Vestec Multi-gauge, Advantec fraction collector Voyager MALDI lasers: rebuilt; full warrantee; ~2/3 price of new; install available. Call for info. Micromass M@LDI LR: $79,000. 2002 linear + reflection benchtop; determine intact protein mass and mPMF. Install included. Mariner ESI-TOF: $45,000. Installed/guar. ok for factory service. MicroMass TOF 2e-Spec: call/email to discuss price. 1999; many options; good working order. MicroMass Q-TOF API-US: call/email to discuss price. 2002; includes CapLC. MicroMass Q-Tof Ultima Global: call/email to discuss price. Complete 2002 system. Micromass Reflectron: $110,000. Incl. MassPrep enclosed robotic sampler system. + $30,500/install. Micromass Q-Tof 2: $90,000. 2000 model; Nanoflow-Z CapElectrophoresis electrospray. Guaranteed installable. Micromass Q-Tof 1: $155,000. + $41K/install and license. Incl. CapLC, many upgrades and extras. MicroMass LC-TOF: $90,000. API-LC/Orth. 2000 model. Manufacturer-certified. Micromass LCT. ~$155,000. API-TOF. Includes HPLC. New July 2000. Sequenom System: $215,000. 2001 model. Bruker BiFlex III, Oracle software, SpectroCheck, Reader and Jet. Installation incl. Sequenom MassARRAY: offers considered. 7K system (2002), for genotyping. Current software. Bruker MicrOTOF: $195,000. 2004 model; lease default. Incl. install, warranty. Bruker Ultraflex: call/email to discuss price. Working perfectly in lab Bruker Reflex IV: $207,500. 2001 model; list $300K. Incl. ion source, TOF analyzer, detector, two NT processing stations. Bruker OmniFlex: $69,750. 2000 model; all hardware upgrades; factory install, warranty included. Bruker Reflex III: $89,000. 1999 model; includes chiller, standalone AS-90. Bruker Biflex III: various systems available; call or email to discuss Bruker Proflex III: offers considered. Excellent condition. LaserTec II: $75,000. By PerSeptive. 5 yrs. old; excellent condition. Thermo-Finnigan Dynamo: $55,000. Linear DE benchtop system; 2 yrs. old; pos/neg. Price includes ship, install, train, 90-day warr. Finnigan LaserMAT 2000: $25,000. Includes ship, install, 90-day parts warr. Kratos Kompact III: offers considered; call or email if interested. SRI custom design: $100,000. Ideal for SNP determination. Asking price. 384 samples/20 min. Can be tested. Finnigan MAT Vision 2000: $80,000. Reflectron. Includes install, 1 year warranty. VG Tof-Spec: $6,000. Or best offer. For parts; new laser card and other new boards. Waters MALDI prep device. Offers considered. Used only once. Includes plates and kits. *Also available: service/contracts on Voyager DE, DE RP and PRO. **GC/Other MS: Micromass VG70 SE: $45,000. Hi resolution GC/MS MSI Autoconcept: $380,000. New; multiramp temp; Agilent GC and Agilent or CTC autosampler. Includes install, 1 yr. warranty. Thermoquest GCQ: $30,000. GC/MS/MS; EI/CI; rebuilt; 90-day warranty. Agilent 5973/6890: $65,000. EI/CI/NCI; one year warranty. HP 5890 II/5970B: Offers considered. SmartCard II, software incl. HP 5989 MS engine: $28,500. 2000 amu mass range, pos/neg CI, APCI. Finnigan Trace 2000: offers considered. 1998. EI/CI, autosampler, NIST library, Xcalibur Finnigan Magnum GC/MS Ion trap: $7,500. Incl. GC. Finnigan MAT GCQ: $23,000. GC/MS/MS. EI/CI. Finnigan MAT SOLA: $50,000. Asking price. 8 yrs. old; incl. GF, hydrides gen. Finnigan T-30 Newstar. Call to discuss price. FT-MS. Was $1.4M in 1997. Price negotiable. JOEL HX 110. Call to discuss price. Tandem Mass spec. Micromass Platform ICP-MS: offers considered. 2001 model; 3rd collision gas option. Regards, Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: JLshen2000@yahoo.ca Date: 13 Jan 2005 08:19:50 -0800 Subject: ESI mechanism Organization: * Dear fellow MSpectrometrists, I would like a few questions: 1. What happens if source temperature is very high and all solvents are evaporated in the source in ESI (turboionspray)? 2. I have seen that lower LC flow requires higher ESI spray voltage. Why is it so? What affects the optimum spray voltage? Thanks in advance! Jimmy ****************************************************************************** From: Richard Barker Date: 13 Jan 2005 09:32:50 -0800 Subject: For Sale: Bruker Reflex IV MALDI TOF system Organization: * Hi We have the following Bruker Mass Spec available for immediate sale: Reflex IV MALDI TOF - Scout 384-MTP Maldi Ion Source in linear, - reflectron PSD-FAST configuration - MAP-II sample preparation robot S/N: 26640.00034 - Desktop PC with NT 4 - FlexControl 1.1 (1 Licence) - Xmass NT 5.1.5 (1 Licence) - BioTools 2.0 (2 Licences) - Gilson Micro 215 XYZ Robot - Year installed 2001 We also have: Biorobotics Microgrid II microarrayer system Model MGII600 S/N: 14144 - PC, Monitor & kb Pyrosequencing PSQ96 Pyrosequencer - HP Vectra PC, Nokia TFT Monitor & kb - HP laserjet printer - Year 2000 ABI Prism 3700 DNA Analyzer - Year 2000 Qiagen Biorobot 8000 Liquid Handling System - PC, monitor & keyboard - Grant LVF6 Low temperature bath + high performance pump - Year 2000 2 x Amersham MegaBACE 4000 BL SEQ WW 384 Capillary System - Year 2002 Photos and prices available on request. Best wishes, Richard Barker richardbarker@assetpoint.co.uk AssetPoint Europe Ltd Tel: 0044 (0)870 366 6150 Fax: 0044 (0)870 922 3109 Mobile: 0044 (0)7799 476 433 ****************************************************************************** From: Kermit Murray Date: 13 Jan 2005 14:04:37 -0800 Subject: Mass Spectrometry Terms Project Organization: * As many of you may know, I am chairing an IUPAC Task Group that is aimed at updating the list of standard mass spectrometry terms. The IUPAC project page is http://www.iupac.org/projects/2003/2003-056-2-500.html. I have set up a website at http://www.msterms.com/ that contains the old list of terms from the IUPAC "Gold Book" and the growing list of new and redefined terms. It's a Wiki, so every page can be edited (logon required) and has an associated discussion page. Take a look at the old and new terms and feel free to comment on the site or send me or any of the Task Group members an e-mail (contact info under the IUPAC link above). Kermit Murray ****************************************************************************** From: meiling li Date: Fri, 14 Jan 2005 17:24:38 -0800 (PST) Subject: Troubleshooting API 2000 oil leakage Organization: * Hi, Currently our API 2000 had its source and analyzer (the vacuum chamber) flooded by unknown oil. I said unknown, that is because we are not sure about where the oil came from. the roughing pump pipe which connect the the MS is perfectly clean (not a single drop of oil), the turbo pump is clean too. Also there is NOT a possible that the oil came from the HPLC. The nitrogen supply is four nine pure nitrogen. It is really a puzzle for our lab. I just wonder maybe any of you can give a smarter answer about where the oil came from. I really appreciate your time. Best regards, Meiling ________________________________________________________________________________ Do you Yahoo!? The all-new My Yahoo! ^Ö What will yours do? ****************************************************************************** From: rglab Date: Mon, 17 Jan 2005 13:35:36 +0100 Subject: Re: Troubleshooting API 2000 oil leakage Organization: * meiling li wrote: } Currently our API 2000 had its source and analyzer (the vacuum chamber) } flooded by unknown oil. I said unknown, that is because we are not sure } about where the oil came from. the roughing pump pipe which connect the } the MS is perfectly clean (not a single drop of oil), the turbo pump is } clean too. Also there is NOT a possible that the oil came from the HPLC. We had such problem two years ago. Please, check if the roughing pump exhaust pipe is separated from the exhaust pipe from the spectrometer. If they are connected then it is possible that the oil vapour is going back to the spectrometer. Regards, Ryszard ****************************************************************************** From: rglab Date: Mon, 17 Jan 2005 13:35:36 +0100 Subject: Re: Troubleshooting API 2000 oil leakage Organization: * meiling li wrote: } Currently our API 2000 had its source and analyzer (the vacuum chamber) } flooded by unknown oil. I said unknown, that is because we are not sure } about where the oil came from. the roughing pump pipe which connect the } the MS is perfectly clean (not a single drop of oil), the turbo pump is } clean too. Also there is NOT a possible that the oil came from the HPLC. We had such problem two years ago. Please, check if the roughing pump exhaust pipe is separated from the exhaust pipe from the spectrometer. If they are connected then it is possible that the oil vapour is going back to the spectrometer. Regards, Ryszard ****************************************************************************** From: SMHalloran Date: Tue, 18 Jan 2005 07:51:14 GMT Subject: Glass vs. Plastic [Stock Chemical] Containers Organization: * In doing any peptide/protein work on MALDI-MS or ESI-MS, does anyone know of a reason to avoid at most every step the use of plastic (polypropylene) tubes and sticking with glass, whether it is sample or reagent? I am scrupulously avoiding the use of acetonitrile that has been sitting for any length of time in a plastic container. I will re-use a plastic container for MeCN, thinking that by dumping out the solvent that has been sitting there for some time and replacing it with fresh material, that much of the materials that can leach out will have already done so. However, I am being given in my requests for reagents (such as concentrated HCl, ethanol, formic acid) these products in plastic bottles by what I think is a popular European manufacturer. Should I be concerned, and should I always insist on a manufacturer that supplies these reagents in glass? ****************************************************************************** From: David Stranz Date: Wed, 19 Jan 2005 09:40:18 -0600 Subject: Re: Glass vs. Plastic [Stock Chemical] Containers Organization: * SMHalloran wrote in news:csj1sk$7vp$1@news-int.gatech.edu: } } In doing any peptide/protein work on MALDI-MS or ESI-MS, does } anyone know of a reason to avoid at most every step the use of } plastic (polypropylene) tubes and sticking with glass, whether } it is sample or reagent? } } I am scrupulously avoiding the use of acetonitrile that has been } sitting for any length of time in a plastic container. I will } re-use a plastic container for MeCN, thinking that by dumping } out the solvent that has been sitting there for some time and } replacing it with fresh material, that much of the materials } that can leach out will have already done so. } } However, I am being given in my requests for reagents (such as } concentrated HCl, ethanol, formic acid) these products in } plastic bottles by what I think is a popular European } manufacturer. Should I be concerned, and should I always insist } on a manufacturer that supplies these reagents in glass? } } The usual reason is the presence of phthalates in the plastic, which will leach out. While these will give you nice peaks for internal mass calibration, maybe that's all you'll see... ion supression in MALDI, high background in ESI. As you may have read in other coments in this NG, even if you use glass, you can get phthalate contamination from the plastic seals in the bottle caps. David ****************************************************************************** From: Kendall Date: Thu, 20 Jan 2005 01:23:24 GMT Subject: Re: Glass vs. Plastic [Stock Chemical] Containers Organization: * "SMHalloran" wrote in message news:csj1sk$7vp$1@news-int.gatech.edu... } } In doing any peptide/protein work on MALDI-MS or ESI-MS, does anyone know } of a reason to avoid at most every step the use of plastic (polypropylene) } tubes and sticking with glass, whether it is sample or reagent? if you're looking at low levels of protein/peptide, you do not want to use glass vials for your samples. proteins will adhere much more readily to glass than they will to plastic Eppendorf tubes (for example) and you'll get no signal. speaking of Eppendorf tubes ... i also found out (the hard way) that all plastic 1.5mL centrifuge tubes are not equal. for MALDI sample prep I typically would prepare martix solution in 1.5mL Eppendorf tubes (saturated matrix in 50%acn, 0.1% TFA). to save a couple of bucks i bought a few thousand non-Eppendorf 1.5mL tubes (according to manufacturer, the same as Eppendorf tubes). lo and befold, when i made matrix solution with these tubes and spotted the MALDI plate, the spots wouldn't dry! big time leaching problem, i imagine, with the off brand tubes. lesson: it it ain't broke, don't fix it. } I am scrupulously avoiding the use of acetonitrile that has been sitting } for any length of time in a plastic container. I will re-use a plastic } container for MeCN, thinking that by dumping out the solvent that has been } sitting there for some time and replacing it with fresh material, that } much } of the materials that can leach out will have already done so. i don't believe i've ever heard of someone storing acetonitrile in plastic (until now) - seems like a bad idea. } However, I am being given in my requests for reagents (such as } concentrated } HCl, ethanol, formic acid) these products in plastic bottles by what I } think is a popular European manufacturer. Should I be concerned, and } should I always insist on a manufacturer that supplies these reagents in } glass? i would vote for glass for all of these, especially for ESI applications. it doesn't take much contaminant to suppress the hell out of your signal! -kp ****************************************************************************** From: IET Ltd Date: 23 Jan 2005 15:37:56 -0800 Subject: Refurbished Analytical Instruments Available Organization: * IET Ltd. is an authorized Thermo Finnigan Reseller *THERMO FINNIGAN NEW ARRIVALS* Thermo Finnigan DECA XP PLUS LC/MS/MS Thermo Finnigan DECA LC/MS/MS Thermo Finnigan Quantum LC/MS/MS Thermo Finnigan AQA LC/MS Thermo Finnigan LCQ Classic LC/MS/MS Thermo Finnigan TSQ 7000 LC/MS/MS with API I More IET MASS SPECTROMETERS Micromass Quattro Ultima LC/MS/MS Micromass Q-TOF USA Applied Biosystems Sciex API 2000 LC/MS/MS Applied Biosystems Sciex API 150 MCA LC/MS Shimadzu LCMS 2010 BIOTECHNOLOGY Applied Biosystems Prism 7900 HT Real Time PCR Cohesive 2303 HTLC with CTC HTS Pal Perceptive Biosystems Voyager-DE STR Hewlett Packard 1100 isocratic pumps Beckman Coulter LS 200 particle size analyzer Genomics Solutions GeneTAC Hybridization Station Perkin Elmer Pyris 1 DSC Waters 650E Advanced Protein Purification System NMR Varian Mercury MVX 300 MHz NMR Varian Gemini 200 NMR Spectrometer Varian Inova 500 MHz NMR Varian Unity 500 MHz NMR Bruker AC 300 Plus NMR Spectrometer Bruker Avance DMX 300 MHz NMR Bruker 3mm NMR MicroCryoProbe (500 MHz) Jeol 400 MHz Eclipse and FT NMR Spectrometer ELECTRON MICROSCOPES JEOL JSM-5600 LV Scanning Electron Microscope Jeol 6300F Scanning Electron Microscope For a complete list of our current inventory, please visit us on the web at http://www.ietltd.com or give us a call at 001.847.913.0777 or 1.800.438.4522. International Equipment Trading Ltd. 960 Woodlands Parkway Vernon Hills, IL 60061 http://www.ietltd.com Ph: 847.913.0777 Fax: 847.913.0785 sales@ietltd.com ****************************************************************************** From: Dana Reed Date: Mon, 24 Jan 2005 17:53:23 -0600 Subject: listing of academic mass spectrometry facilities Organization: * Hello, I have been searching the web for listings of academic ms facilities, and have found many lists that are out of date or have lots of broken links. I have started a list of my own here. http://www.chem.umn.edu/services/massspec/facilities.html I'm sure this is not a complete list (just generated from googling around), so I've posted here to get feedback about any glaring omissions. Also, I'm wondering how many subscribers here are managing facilities of this type, and if a moderated email list discussing issues facing these facilites would be of interest. (Student/User training, departmentmental/institutional subsidy, recharge rates, software issues, etc.) Thanks, Dana ****************************************************************************** From: J T Hill Date: Tue, 25 Jan 2005 10:57:18 +0000 Subject: Ceasium Gun Organization: * Hiya all Doe anyone know the address and fax number of Antek in California who make the ceasium ion pellets for FAB guns? They don't seem to have a website. Are they made by anyone else? The US dollar is up at 1.87 to pound sterling at the moment so will pay us to buy a few direct. Thanks for your time. Regards John John Hill Mass Spectrometry Facility University College London Chemistry Department 20 Gordon St. London WC1H 0AJ Tel: 020 7679 4605 Fax: 020 7679 7451 Mobile: 07956 924058 ****************************************************************************** From: Crabs Date: Tue, 25 Jan 2005 06:52:59 -0800 Subject: Re: Ceasium Gun Organization: * J T Hill wrote: } Hiya all } } Doe anyone know the address and fax number of Antek in California who make } the ceasium ion pellets for FAB guns? They don't seem to have a website. } Are they made by anyone else? } } The US dollar is up at 1.87 to pound sterling at the moment so will pay us } to buy a few direct. } } Thanks for your time. } } Regards } } John } } } John Hill } Mass Spectrometry Facility } University College London } Chemistry Department } 20 Gordon St. } London } WC1H 0AJ } } Tel: 020 7679 4605 } Fax: 020 7679 7451 } Mobile: 07956 924058 } } John: Antek 3146 Manchester Court Palo Alto, CA 94303 (650)856-2412 Regards, TomC ****************************************************************************** From: jduan111@yahoo.com Date: 25 Jan 2005 13:22:02 -0800 Subject: scan width and peak width Organization: * hi, there, I have one question, maybe very stupid one. I am very confused with the parameters of scan width and peak width in quadrupole mass spectrometry. Sometimes, I saw people set scan width at 0.1amu, but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width is only 0.1amu, why set the peak width at 0.7? The peak width refers to the m/z peak width, right? Or how these two parameters correlate with each other in quadrupole mass spectrometer? Is there anyone kindly enough to explain that to me? I am very confused about that, though I know it is a very simple question. Thanks so much! jin ****************************************************************************** From: Kajjo Date: 26 Jan 2005 06:27:18 -0800 Subject: LECO ChromaTOF data file format Organization: * Hi there! We would like to automatically process our data files acquired with a LECO ChromatoTOF system (Pegasus III). Does anyone know the binary data file format of the *.peg oder *.smp files generated? Many thanks in advance, Kajjo ****************************************************************************** From: "rainer [iso-8859-2] lörwald" Date: Wed, 26 Jan 2005 22:51:58 +0100 Subject: Re: scan width and peak width Organization: * Jin, these settings are good for most quad instruments not capable of higher resolution without loosing too much of the signal´s intensity (a factor of 2-3 is acceptable). A peak width of 0.7 amu (which is measured at half peak height) means "unit resolution", i.E masses M and M+1 of singly charged ions can be seperated. Centering the scan on top of the peak with a width of 0.1 amu will give you most of the signal´s intensity (simply draw a picture of a peak with 0.7 amu width and the section of 0.1 amu on top of it) The reason for the small scan width is the increase in acquisition time for that small part of the peak at a constant total time compared to the time needed for a scan over the whole peak (1 amu). You will not loose much intensity if not looking at the slopes of the peak, but S/N increases by a factor sqrt(10:1). The only thing that is important is the stability of the calibration and the correct setting of the mass of the observed peak to be sure to be on top of it. Take a piece of paper and visualize for yourself what happens! Using an instrument with the capability of higher resolution will give a higher increase in S/N due to elimination of contaminants with same nominal but different exact mass. HTH Rainer jduan111@yahoo.com schrieb: } } hi, there, I have one question, maybe very stupid one. I am very } confused with the parameters of scan width and peak width in quadrupole } mass spectrometry. Sometimes, I saw people set scan width at 0.1amu, } but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width is } only 0.1amu, why set the peak width at 0.7? The peak width refers to } the m/z peak width, right? Or how these two parameters correlate with } each other in quadrupole mass spectrometer? } Is there anyone kindly enough to explain that to me? I am very confused } about that, though I know it is a very simple question. } Thanks so much! } } jin ****************************************************************************** From: jduan111@yahoo.com Date: 26 Jan 2005 14:49:48 -0800 Subject: Re: scan width and peak width Organization: * Thanks so much!!! That make sense!, jin jduan111@yahoo.com wrote: } hi, there, I have one question, maybe very stupid one. I am very } confused with the parameters of scan width and peak width in quadrupole } mass spectrometry. Sometimes, I saw people set scan width at 0.1amu, } but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width is } only 0.1amu, why set the peak width at 0.7? The peak width refers to } the m/z peak width, right? Or how these two parameters correlate with } each other in quadrupole mass spectrometer? } Is there anyone kindly enough to explain that to me? I am very confused } about that, though I know it is a very simple question. } Thanks so much! } } jin ****************************************************************************** From: Fred&Nat Date: Thu, 27 Jan 2005 01:18:51 +0100 Subject: Re: Troubleshooting API 2000 oil leakage Organization: * The only source of oil is the roughing pump so there must be a return from the exhaust or something like that ! "meiling li" a écrit dans le message news: csa0q8$irc$1@news-int2.gatech.edu... } } Hi, } } Currently our API 2000 had its source and analyzer (the vacuum chamber) } flooded by unknown oil. I said unknown, that is because we are not sure } about where the oil came from. the roughing pump pipe which connect the } the MS is perfectly clean (not a single drop of oil), the turbo pump is } clean too. Also there is NOT a possible that the oil came from the HPLC. } The nitrogen supply is four nine pure nitrogen. It is really a puzzle for } our lab. I just wonder maybe any of you can give a smarter answer about } where the oil came from. I really appreciate your time. } } Best regards, } } Meiling } } } ____________________________________________________________________________ ____ } Do you Yahoo!? } The all-new My Yahoo! ^Ö What will yours do? } ****************************************************************************** From: jduan111@yahoo.com Date: 26 Jan 2005 21:07:36 -0800 Subject: Re: scan width and peak width Organization: * rainer [iso-8859-2] lörwald wrote: } Jin, } } these settings are good for most quad instruments not } capable of higher resolution without loosing too much of the } signal´s intensity (a factor of 2-3 is acceptable). A peak } width of 0.7 amu (which is measured at half peak height) } means "unit resolution", i.E masses M and M+1 of singly } charged ions can be seperated. Centering the scan on top of } the peak with a width of 0.1 amu will give you most of the } signal´s intensity (simply draw a picture of a peak with 0.7 } amu width and the section of 0.1 amu on top of it) The } reason for the small scan width is the increase in } acquisition time for that small part of the peak at a } constant total time compared to the time needed for a scan } over the whole peak (1 amu). You will not loose much } intensity if not looking at the slopes of the peak, but S/N } increases by a factor sqrt(10:1). } The only thing that is important is the stability of the } calibration and the correct setting of the mass of the } observed peak to be sure to be on top of it. } Take a piece of paper and visualize for yourself what } happens! } Using an instrument with the capability of higher resolution } will give a higher increase in S/N due to elimination of } contaminants with same nominal but different exact mass. Excuse me, I just wonder what do you mean by "elimination of contaminants with same nominal but different exact mass"? Why could that happen by increasing resolution?What is the theory behind that? Thanks so much for your patient answer, look forward to hearing from you, thanks, jin } } } HTH } Rainer } } } jduan111@yahoo.com schrieb: } } } } hi, there, I have one question, maybe very stupid one. I am very } } confused with the parameters of scan width and peak width in quadrupole } } mass spectrometry. Sometimes, I saw people set scan width at 0.1amu, } } but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width is } } only 0.1amu, why set the peak width at 0.7? The peak width refers to } } the m/z peak width, right? Or how these two parameters correlate with } } each other in quadrupole mass spectrometer? } } Is there anyone kindly enough to explain that to me? I am very confused } } about that, though I know it is a very simple question. } } Thanks so much! } } } } jin ****************************************************************************** From: Achim Treumann Date: Thu, 27 Jan 2005 19:20:17 -0000 Subject: Senior Research Technician Mass Spectrometry Organization: * Senior Technician Ref HR50021 At the Mass Spectrometry Core, Royal College of Surgeons in Ireland. We are looking for a senior technician to support the activities of the mass spectrometry core at the Royal College of Surgeons in Ireland (RCSI). The RCSI is a thriving teaching and research institution in the centre of one of the liveliest cities in Europe. Mass spectrometry and proteomics are key factors of the research strategy of the RCSI and you will play a major role in their expansion. You will operate and maintain the mass spectrometry and chromatography equipment in a new, purpose built mass spectrometry laboratory, develop and implement methods for the analysis of biopolymers (proteins, peptides and nucleic acids) and of small molecules (lipids, metabolites, drugs) by MS and train and supervise junior staff and students. Essential requirements: tertiary qualification in analytical chemistry, biochemistry or a different relevant subject, minimum of 4 years of hands-on experience in quantitative analytical science and in mass spectrometry, broad experience with the operation and troubleshooting of chromatography and mass spectrometry equipment, good biochemical knowledge, good written and verbal communication skills, computing skills, team player, able to operate in a multi- disciplinary environment. Desirable qualifications: M.Sc. or higher academic qualification, experience in the supervision of small teams, knowledge and experience in synthetic organic chemistry, proteomics experience. The Royal College of Surgeons in Ireland is fully committed to equality of opportunity, which is an integral part of its overall policy. For informal enquiries please contact Achim Treumann, Director of the Mass Spectrometry Core, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin 2, Ireland, Tel. +353 1 4022330, email atreumann@n0spam.rcsi.ie APPLICATION PROCEDURE Please forward a copy of your curriculum vitae together with a covering letter quoting ref HR50021 and the names and contact details of three referees to: Ms. Y. Costelloe, HR Department, 121, St. Stephen's Green, Dublin 2. Tel: (00353 1) 402 2440 email: recruit@rcsi.ie The closing date for receipt of applications is Closing date for applications is 5 pm, February 28th, 2005. www.rcsi.ie -------------------------------------------------------------------------------------------------------------------- This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of The Royal College Of Surgeons in Ireland. -------------------------------------------------------------------------------------------------------------------- ****************************************************************************** From: "rainer [iso-8859-2] lörwald" Date: Fri, 28 Jan 2005 00:11:11 +0100 Subject: Re: scan width and peak width Organization: * Jin, simply look at N2 and CO both having a nominal mass of 28. Looking at them at 0.7 amu peakwidth you will see one signal. Calculation of the exact masses yields 28.004 for N2 and 27.996 for CO. Mass defect is the key to this. Increasing resolution will show you two peaks and enable you to quantitate the one of your interest (in this case CO as its abundance is very low) without any interference of the other (or, in this case, not even monitoring any difference regardless of a 10fold increase in CO concentration except your system is really leak free). For this example you will need a much higher resolution than achievable on a quad but this pair is often used on hires magnetic instruments to check resolution. For normal organic compounds in the mid mass range (say above 100 amu) it is often possible to distinguish between compound of interest and background signal through the use of higher resolution even on a quad MS. Reducing the amount of the contamination in your signals will result in a better S/N value. My advice: Don´t use the MS as a simple detector, acquire more knowledge about it by either reading good books or attending a training course, and you will find out that it is not as easy to operate as a normal detector but it is much more powerfull. HTH Rainer jduan111@yahoo.com schrieb: } } rainer [iso-8859-2] lörwald wrote: } } Jin, } } } } these settings are good for most quad instruments not } } capable of higher resolution without loosing too much of the } } signal´s intensity (a factor of 2-3 is acceptable). A peak } } width of 0.7 amu (which is measured at half peak height) } } means "unit resolution", i.E masses M and M+1 of singly } } charged ions can be seperated. Centering the scan on top of } } the peak with a width of 0.1 amu will give you most of the } } signal´s intensity (simply draw a picture of a peak with 0.7 } } amu width and the section of 0.1 amu on top of it) The } } reason for the small scan width is the increase in } } acquisition time for that small part of the peak at a } } constant total time compared to the time needed for a scan } } over the whole peak (1 amu). You will not loose much } } intensity if not looking at the slopes of the peak, but S/N } } increases by a factor sqrt(10:1). } } The only thing that is important is the stability of the } } calibration and the correct setting of the mass of the } } observed peak to be sure to be on top of it. } } Take a piece of paper and visualize for yourself what } } happens! } } Using an instrument with the capability of higher resolution } } will give a higher increase in S/N due to elimination of } } contaminants with same nominal but different exact mass. } } Excuse me, I just wonder what do you mean by "elimination of } contaminants with same nominal but different exact mass"? Why could } that happen by increasing resolution?What is the theory behind that? } } Thanks so much for your patient answer, look forward to hearing from } you, thanks, } } jin } } } } } } HTH } } Rainer } } } } } } jduan111@yahoo.com schrieb: } } } } } } hi, there, I have one question, maybe very stupid one. I am very } } } confused with the parameters of scan width and peak width in } quadrupole } } } mass spectrometry. Sometimes, I saw people set scan width at } 0.1amu, } } } but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width } is } } } only 0.1amu, why set the peak width at 0.7? The peak width refers } to } } } the m/z peak width, right? Or how these two parameters correlate } with } } } each other in quadrupole mass spectrometer? } } } Is there anyone kindly enough to explain that to me? I am very } confused } } } about that, though I know it is a very simple question. } } } Thanks so much! } } } } } } jin ****************************************************************************** From: Rich Yelton Date: Sat, 29 Jan 2005 06:24:56 GMT Subject: Re: scan width and peak width Organization: * The mass spectrometer data system acquires data by scanning digitally, or stepwise across a mass peak.The scan width refers to the step size of each digital cycle across the mass peak. In your case you are setting this to 0.1 amu therefore you are acquiring 10 samples across each mass peak that are 0.1 amu wide. When you set Q1 and Q3 to 0.7 amu, you are now controlling the electronics on each quadrupole that controls the ratio of RF to DC voltages applied to the rods to obtain a mass peak width of 0.7 amu at half height. In this case we are referring to mass peak resolution. Rich wrote in message news:ct6evc$q72$1@news-int2.gatech.edu... } hi, there, I have one question, maybe very stupid one. I am very } confused with the parameters of scan width and peak width in quadrupole } mass spectrometry. Sometimes, I saw people set scan width at 0.1amu, } but Q1 or Q3 peak width at 0.7amu. I just wonder if the scan width is } only 0.1amu, why set the peak width at 0.7? The peak width refers to } the m/z peak width, right? Or how these two parameters correlate with } each other in quadrupole mass spectrometer? } Is there anyone kindly enough to explain that to me? I am very confused } about that, though I know it is a very simple question. } Thanks so much! } } jin } } ****************************************************************************** From: Mike Sherrell Date: Mon, 31 Jan 2005 13:52:30 -0800 Subject: MSs and MALDIs available Organization: * Mass specs and MALDIs available from Grizzly Analytical: **LC/MS & MS/MS: Q-Star Pulsar: $170,000. Not the "i". Recently pmd. + $16K/factory install. Q-Star Pulsar XL: Call/email to discuss price. Hybrid Q-TOF. Sciex API 4000: $265,000. Running in lab now; can be tested. API 3000 upgrade: $38,500. Increases sensitivity and (S/N) Ratio at high flow rates to ~ that of an API 4000. Install incl. Sciex API 2000: $70,000 installed and warranteed. Sciex API 2000: $120,000: Incl. EPQ3 upgrade to ~ API 3000 sensitivity, install & Warranty. Sciex API 2000 upgrade: $25,000. 4x sensitivity increase; incl. install, warr. Sciex API 365: $65,000. NT, turboionspray. Installed, warranteed. Sciex 365 w/ EP10+: $149,000. Custom upgrade; more sensitive than API 3000. Sciex API 365 upgrade: $109,000. 10x+ sensitivity upgrade; near-equal to 4000. Sciex API 150EX: $48,000. MCA upgraded to EX; identical performance. MAC; NT + $10,000. Incl. install. Sciex NT workstation: $2,500. Use to upgrade Macs on 150, 365, 2000, 3000. Sciex API III+: $25,000. Triple quad: ES, APCI; +$24K/intall w/ 1 yr. warr. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $20,000/installation and 1 year service contract. Sciex Turboionspray source (aka ESI) for API 150, 365 or 3000: $6,250. Sciex MicroIon spray source: $7,900. For API 150, 300 or Q-Star. Very low flow. PE-ABI Mariner: $45,000. Price includes factory install. Agilent 1100 MSD Trap. Call to discuss price. 2001 model. Agilent 1100 MSD: $various. All Models (A - D) with varying sources (ESI, APCI, APPI). Most any configuration. Can include install, 90-day warranty and training. Agilent 1100 VL LC/MSD: offers considered. 2004 system. Finnigan Quantum AM: $320,000. 2004 system. Incl. install, 1 yr. svc. Finnigan Quantum Original: $160,000. ESI only (APCI available); factory refurbed; guaranteed installable. Install/warranty avail. Finnigan Discovery Max: $179,000. ew; specs comparable to Sciex API 3000. Incl. install, 1 year svc. Finnigan Deca XP+: $150,500. 2002; pristine; installed and calibrated but never used. Includes factory install, guar. eligible for svc. Finnigan Deca XP: $135,000. 30000 model. Includes install and 1 year svc. Factory upgrade to XP + for addt'l $14,000. Finnigan LCQ DECA: $67,500. ESI, Xcalibur 1.2, install, warranty incl. Finnigan LCQ DUO: $75,000 installed. Finnigan LCQ Classic: $58,500. ESI, installation, 90-day warr. LCQ Classic ESI source: $7,500. New/unused ESI source. Finnigan Navigator: $29,500. Single quad. Installed, 30-day warr. Finnigan TSQ 7000: $50,000. ES, Xcalibur software, installation and 30-day warranty. Workhorse triple quad. Finnigan TSQ 7000 GC/MS: $65,000. GC and LC-capable; Xcalibur. Install, warr. avail. Finnigan SSQ 7000: $45,000. ES, APCI; Excalibur 1.0; API 1 source; install incl. Finnigan TSQ 700: $30,000. Electrospray, APCI. Install included. Finnigan SSQ 710: $25,000. Electrospray, APCI, API 1, Alpha workstation, install included. Finnigan Mat ITS40W. Call to discuss price. With Varian 3400 GC + A200s Auto Sampler. Bruker Esquire: $85,000. Ion trap. Incl. install, guaranteed. Hitachi M 8000: $82,000. Ion trap. 1999; excellent condition; incl. LC. Micromass Quattro micro API: $150,000; Complete 2000. On factory service contract when deinstalled. Micromass LCT API-oaTOF MS: $160,000. Sold new for $260,000 in July 2000. Includes Waters HPLC. Micromass Quattro LCZ: $131,500. Includes installation, warranty. Micromass Quattro IIZ: $149,000. Z-Spray. Includes install, warranty. Micromass Quattro Ultima: $125,000. VE. Z-Spray, APCI-Z, new MassLynx workstation. Micromass Quattro II: $150-200K. Price depends on whether you want installation, GC and/or HPLC. Micromass Q-Tof II: $185,000. Hybrid Quadrupole. Installed, eligible for service contract. MM/Waters ZMD 4000: $49,000. ESI, APCI. +$10K/factory install. Micromass ZABSpec Ultima OA TOF: $89,000. Mag sector/TOF hybrid. 1997 model. Good working order. Micromass Autospec M: $179,000. Mag sector/TOF MS/MS. Incl. FPD, 5 sources, guar. eligible for svc. ($700K new) Shimadzu 2010: offers considered. 2001 system; single quad; ESI/APCI. Fisons VG 2000. <$100,000. Fisons VG Trio: $25,000. LC + GC: 3000 amu; thermospray, EI/CI, HP 5890 included. Install, license & 90-day warr. + $14,500. VG Trio 2: $7,500. Electrospray; complete; parts or fixer-upper unit. Nermag R10c. <$10,000. Like new; make offer. Finnigan MAT 90: $16,000. All parts intact, plus spares included. MM Autospec: call if interested. Mag Sector. TOF, MSMS, ESI, MALDI, EI/CI. MM Autospec S: $60,000. European install included; available in US. MM Autospec V: $70,000. European install included; available in US. Mass spec sample introduction systems listed under liquid handlers, below. *Service and service contracts available for PESciex API 3000, 365 and III+. **MALDI-TOFs: Voyager DE: $45,000. Installed, guaranteed. Voyager DE STR: $138,500. Includes install; guaranteed eligible for svc contract. Voyager DE Pro: $109,000. Incl. factory install, certification. Voyager DE RP: $67,000. Extensively refurbished. Voyager RP: $44,000. Incl. install; service contracts avail. Will QC peptides; continuous extraction. Voyager Elite XL: offers considered. incl. Vestec Multi-gauge, Advantec fraction collector Voyager MALDI lasers: rebuilt; full warrantee; ~2/3 price of new; install available. Call for info. Micromass M@LDI LR: $79,000. 2002 linear + reflection benchtop; determine intact protein mass and mPMF. Install included. Mariner ESI-TOF: $45,000. Installed/guar. ok for factory service. MicroMass TOF 2e-Spec: call/email to discuss price. 1999; many options; good working order. MicroMass Q-TOF API-US: call/email to discuss price. 2002; includes CapLC. MicroMass Q-Tof Ultima Global: call/email to discuss price. Complete 2002 system. Micromass Reflectron: $110,000. Incl. MassPrep enclosed robotic sampler system. + $30,500/install. Micromass Q-Tof 2: $90,000. 2000 model; Nanoflow-Z CapElectrophoresis electrospray. Guaranteed installable. Micromass Q-Tof 1: $155,000. + $41K/install and license. Incl. CapLC, many upgrades and extras. MicroMass LC-TOF: $90,000. API-LC/Orth. 2000 model. Manufacturer-certified. Micromass LCT. ~$155,000. API-TOF. Includes HPLC. New July 2000. Sequenom System: $215,000. 2001 model. Bruker BiFlex III, Oracle software, SpectroCheck, Reader and Jet. Installation incl. Sequenom MassARRAY: offers considered. 7K system (2002), for genotyping. Current software. Bruker MicrOTOF: $195,000. 2004 model; lease default. Incl. install, warranty. Bruker Ultraflex: call/email to discuss price. Working perfectly in lab Bruker Reflex IV: $207,500. 2001 model; list $300K. Incl. ion source, TOF analyzer, detector, two NT processing stations. Bruker OmniFlex: $69,750. 2000 model; all hardware upgrades; factory install, warranty included. Bruker Reflex III: $89,000. 1999 model; includes chiller, standalone AS-90. Bruker Biflex III: various systems available; call or email to discuss Bruker Proflex III: offers considered. Excellent condition. LaserTec II: $75,000. By PerSeptive. 5 yrs. old; excellent condition. Thermo-Finnigan Dynamo: $55,000. Linear DE benchtop system; 2 yrs. old; pos/neg. Price includes ship, install, train, 90-day warr. Finnigan LaserMAT 2000: $25,000. Includes ship, install, 90-day parts warr. Kratos Kompact III: offers considered; call or email if interested. SRI custom design: $100,000. Ideal for SNP determination. Asking price. 384 samples/20 min. Can be tested. Finnigan MAT Vision 2000: $80,000. Reflectron. Includes install, 1 year warranty. VG Tof-Spec: $6,000. Or best offer. For parts; new laser card and other new boards. Waters MALDI prep device. Offers considered. Used only once. Includes plates and kits. *Also available: service/contracts on Voyager DE, DE RP and PRO. **GC/Other MS: Micromass VG70 SE: $45,000. Hi resolution GC/MS MSI Autoconcept: $380,000. New; multiramp temp; Agilent GC and Agilent or CTC autosampler. Includes install, 1 yr. warranty. Thermoquest GCQ: $30,000. GC/MS/MS; EI/CI; rebuilt; 90-day warranty. Agilent 5973/6890: $65,000. EI/CI/NCI; one year warranty. HP 5890 II/5970B: Offers considered. SmartCard II, software incl. HP 5989 MS engine: $28,500. 2000 amu mass range, pos/neg CI, APCI. Finnigan Trace 2000: offers considered. 1998. EI/CI, autosampler, NIST library, Xcalibur Finnigan Magnum GC/MS Ion trap: $7,500. Incl. GC. Finnigan MAT GCQ: $23,000. GC/MS/MS. EI/CI. Finnigan MAT SOLA: $50,000. Asking price. 8 yrs. old; incl. GF, hydrides gen. Finnigan T-30 Newstar. Call to discuss price. FT-MS. Was $1.4M in 1997. Price negotiable. JOEL HX 110. Call to discuss price. Tandem Mass spec. Micromass Platform ICP-MS: offers considered. 2001 model; 3rd collision gas option. HP 4500 ICP-MS: $49,000. Series 200; 1999 model; enhanced mainframe. Regards, Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: "Danis, Claude" Date: Tue, 1 Feb 2005 08:28:06 -0500 Subject: Signal saturation on Finnigan's GCQ Organization: * Good morning all ! Any of you ''ms gurus'' would have a explanation for my problem ? Here's the situation : The trap is not contaminated (absolutely sure), and i've tested different filaments and electron multipliers to give the same problem no matter what ! So far runnning a sample or tuning the ms or just running the ms to check the air/water level and back-ground (gives normal figures) for some reason the signal showed will saturate the intensity (ie 100% of the signal: mass graph) and to top it off the problem is intermittent ! The only way i have found to reestablish the signal to the proper level is to manually put the system switch to ''Service'' for 10-15 seconds and then switch back to ''Normal'' I have run the diagnostic of the system and calibration, everything ok (if the the problem do not appear before the end of the procedure !) Help please Carol ****************************************************************************** From: Lutz Date: 2 Feb 2005 09:20:34 -0800 Subject: Ionization coefficients for several gases Organization: * Hello, I use a quadrupole with an ionization energy of 77eV (EI). Can anybody tell me, where I can find the ionization coefficients (probabilities) for the gases/vapors H2O(18), HF(20), HCN(27), CO(28), NO(30), S(32), CO2(44), NO2(46), SO(48), SO2(64), COF2(66), SO3(80), CF4(88), SO2F2(102), SOF4(124), SF6(146) or at least a few of them? Ionization energies from 70 eV to 100 eV are acceptable, since I need them for a more qualitative than quantitative analysis. Thanks in advance for your efforts. Best regards, Lutz ****************************************************************************** From: oookhc@hotmail.com Date: 2 Feb 2005 16:07:17 -0800 Subject: Re: listing of academic mass spectrometry facilities Organization: * Thanks for that link. You might check the following links to see if you can find some more to add on your list: http://www.ScienceOxygen.com/apparatus99.html http://www.ScienceOxygen.com/apparatus181.html ****************************************************************************** From: David Date: Wed, 2 Feb 2005 22:54:27 -0800 Subject: Looking for new software to analyze data Organization: * Hello, The lab where I work in has a few HP 5890 GC/FID's, GC/NPD's, 5972 GCMS's. We use Agilent Chemstation to collect data from the instruments, but Target software from Thermoelectron Corporation is used to analyze the data. We have been told that Target software will likely be unsupported in a few years. Can anyone suggest a competent replacement for Target software and provide a link to more information. Thanks --David ****************************************************************************** From: Dave White Date: Thu, 3 Feb 2005 22:46:11 -0600 Subject: Re: Looking for new software to analyze data Organization: * "David" wrote in message news:ctt9eq$e53$1@news-int2.gatech.edu... } Hello, } } The lab where I work in has a few HP 5890 GC/FID's, GC/NPD's, 5972 GCMS's. } We use Agilent Chemstation to collect data from the instruments, but Target } software from Thermoelectron Corporation is used to analyze the data. We } have been told that Target software will likely be unsupported in a few } years. Can anyone suggest a competent replacement for Target software and } provide a link to more information. } David, We have been developing a package that may fit your needs. It has been used for production in a large environmental lab for the past few years, and has just been purchased by another well known environmental lab. We're just putting the finishing touches to the system, and working on the documentation, which we expect to take another six to eight weeks. If you'd like more information, please contact me with a valid e-mail address, and I'll forward some more detailed information. Regards Dave White SpectraChrom Software www.spectrachrom.com info@spectrachrom.com ****************************************************************************** From: Lutz Date: 5 Feb 2005 06:32:43 -0800 Subject: EI ionization cross sections for several gases Organization: * Hello, I use a quadrupole with an ionization energy of 77 eV (EI). Can anybody tell me, where I can find the EI ionization cross sections or ionization coefficients (probabilities) for the gases/vapors HF(20), HCN(27), S(32), SO(48), COF2(66), SO3(80), SO2F2(102), SOF4(124) or at least a few of them? Ionization energies from 70 eV to 100 eV are acceptable. I already found the EI ionization cross sections for a lot of molecules and atoms on "http://physics.nist.gov/PhysRefData/Ionization/", which is avery good page. Thanks in advance for your efforts. Best regards, Lutz ****************************************************************************** From: Sim Date: Sun, 6 Feb 2005 19:12:51 +0100 Subject: HELP!! mass spectrometric data conversion Organization: * Hello I am an Italian resercher and I need help. I work with a MALDI (Voyager DE-STR). The spectra are saved in .dat format. I would like to convert these data to .mzXML format but as far as I know it is not possible to direcly convert the .dat into .mzXML format. Do you know if exhist some programs to convert this format to other file formats like .raw .wiff .cdf? These formats, in fact, can be converted to .mzXML with some specific tools and this can be a solution to my problems. If you can help me please reply to me both on this newsgroup and using my E-MAIL: simone.cristoni@virgilio.it Thank you very much for your help. Simone ****************************************************************************** From: Christoph Eisenmenger-Sittner Date: 07 Feb 2005 06:53:58 GMT Subject: Meeting Announcement: ECASIA05 Vienna Organization: * 11th European Conference on Applications of Surface and Interface Analysis (ECASIA05) Vienna (Austria), 25th to 30th September 2005. 2nd announcement - Call for abstracts Dear Colleagues, The 11th European Conference on Applications of Surface and Interface Analysis (ECASIA05) is going to be held in Vienna, Austria, between the 25th and 30th September 2005. The scientific programme comprises all aspects of surface and interface analysis in academic and industrial applications and includes the following topics: - Metals - Oxides - Polymers - Magnetic materials - Biomaterials - Thin films and coatings - Ceramics - Catalytic materials - Micro- and optoelectronics - Nano- (bio-) materials - Solid-liquid interfaces - Surface modification and functionalization - Corrosion - Adhesion - Tribology - Biomaterials - Environmetal analysis - Heritage conservation - Quantification and data interpretation - Imaging - Depth profiling - Simulation and software - Technique development - Standardization Invited and contributed papers shall be presented during plenary, oral and poster sessions. Confirmed invited speakers include J.W. Niemantsverdriet (catalysis, NL), C. Montemagno (nano-bio technology, US), A. Diebold (microelectronis, US), S. Mueller (surface engineering, DE), E. Platzgummer (surface modification, A), E, Paparazzo (heritage conservation, I), M. Kiskinova (chemical imaging, I), D. W. Moon (quantitative ion beam analysis, KR), S. Ichimura (oxidation, J) and Z.- J. Ding (simulation of electron beam techniques, CN). Instrument manufacturers and suppliers of accessories for surface and interface analysis will present recent developments at an exhibition during the conference. For detailed information on the scientific programme, abstract submission and registration please visit the ECASIA05-homepage at http://www.iap.tuwien.ac.at/ecasia05. The abstract submission deadline is 1st March 2005. Looking forward to welcoming you at the ECASIA05 in Vienna, Yours sincerely, Wolfgang Werner Herbert Stoeri (chair) (vice chair) ---------------------------------------------------------------------- ECASIA'05 European Conference on Applications of Surface and Interface Analysis September 25-30, 2005 Vienna University of Technology, Vienna, Austria Chair: Wolfgang SM Werner www: http://www.iap.tuwien.ac.at/ecasia05 e-mail: ecasia05@iap*tuwien*ac*at ---------------------------------------------------------------------- Scientific Committee - P. Bauer, Austria - A. Benninghoven, Germany - P. Cumpson, United Kingdom - A. Gonzalez-Elipe, Spain - I. Gebeshuber, Austria - A. Jablonski, Poland - L. Koever, Hungary - H. Stoeri, Austria - A. Testoni, USA - W.S.M. Werner, Austria - K. Yoshihara, Japan - P.C. Zalm, The Netherlands - A. Zalar, Slovenia Local Organizing Committee - C. Eisenmenger-Sittner, Social Programme - G. Friedbacher, Exhibition/Sponsoring - W. Hofer, IT - H. Hutter, Exhibition/Sponsoring - J. Laimer, Treasurer - M. Marik, Conference Office - F. Ruedenauer, Proceedings - W. Smekal, IT - H. Stoeri, Vice-Chair - W.S.M. Werner, Chair Conference Office Marik Manuela Technische Universitaet Wien Institut fuer Allgemeine Physik / E134 Wiedner Hauptstra§e 8-10 A 1040 Wien Austria Phone: +43 1 58801 13405 Fax: +43 1 58801 13499 e-Mail: ecasia05@iap*tuwien*ac*at For correct e-mail replies please exchange asterisks (*) by dots (.) in all given e-mail addresses ****************************************************************************** From: Davide Cittaro Date: Mon, 7 Feb 2005 11:28:35 +0100 Subject: Re: HELP!! mass spectrometric data conversion Organization: * I don't know this file format, is it binary? Otherwise you can write a short conversion application! Bye davide Sim wrote: } Hello I am an Italian resercher and I need help. } } I work with a MALDI (Voyager DE-STR). The spectra are saved in .dat format. } I would like to convert these data to .mzXML format but as far as I know it } is not possible to direcly convert the .dat into .mzXML format. Do you know } if exhist some programs to convert this format to other file formats like } .raw .wiff .cdf? These formats, in fact, can be converted to .mzXML with } some specific tools and this can be a solution to my problems. } } } If you can help me please reply to me both on this newsgroup and using my } E-MAIL: simone.cristoni@virgilio.it } } Thank you very much for your help. } } Simone -- Davide Cittaro Drop WINDOWS if you want to reply ****************************************************************************** From: Christoph Eisenmenger-Sittner Date: 07 Feb 2005 06:53:58 GMT Subject: Meeting Announcement: ECASIA05 Vienna Organization: * 11th European Conference on Applications of Surface and Interface Analysis (ECASIA05) Vienna (Austria), 25th to 30th September 2005. 2nd announcement - Call for abstracts Dear Colleagues, The 11th European Conference on Applications of Surface and Interface Analysis (ECASIA05) is going to be held in Vienna, Austria, between the 25th and 30th September 2005. The scientific programme comprises all aspects of surface and interface analysis in academic and industrial applications and includes the following topics: - Metals - Oxides - Polymers - Magnetic materials - Biomaterials - Thin films and coatings - Ceramics - Catalytic materials - Micro- and optoelectronics - Nano- (bio-) materials - Solid-liquid interfaces - Surface modification and functionalization - Corrosion - Adhesion - Tribology - Biomaterials - Environmetal analysis - Heritage conservation - Quantification and data interpretation - Imaging - Depth profiling - Simulation and software - Technique development - Standardization Invited and contributed papers shall be presented during plenary, oral and poster sessions. Confirmed invited speakers include J.W. Niemantsverdriet (catalysis, NL), C. Montemagno (nano-bio technology, US), A. Diebold (microelectronis, US), S. Mueller (surface engineering, DE), E. Platzgummer (surface modification, A), E, Paparazzo (heritage conservation, I), M. Kiskinova (chemical imaging, I), D. W. Moon (quantitative ion beam analysis, KR), S. Ichimura (oxidation, J) and Z.- J. Ding (simulation of electron beam techniques, CN). Instrument manufacturers and suppliers of accessories for surface and interface analysis will present recent developments at an exhibition during the conference. For detailed information on the scientific programme, abstract submission and registration please visit the ECASIA05-homepage at http://www.iap.tuwien.ac.at/ecasia05. The abstract submission deadline is 1st March 2005. Looking forward to welcoming you at the ECASIA05 in Vienna, Yours sincerely, Wolfgang Werner Herbert Stoeri (chair) (vice chair) ---------------------------------------------------------------------- ECASIA'05 European Conference on Applications of Surface and Interface Analysis September 25-30, 2005 Vienna University of Technology, Vienna, Austria Chair: Wolfgang SM Werner www: http://www.iap.tuwien.ac.at/ecasia05 e-mail: ecasia05@iap*tuwien*ac*at ---------------------------------------------------------------------- Scientific Committee - P. Bauer, Austria - A. Benninghoven, Germany - P. Cumpson, United Kingdom - A. Gonzalez-Elipe, Spain - I. Gebeshuber, Austria - A. Jablonski, Poland - L. Koever, Hungary - H. Stoeri, Austria - A. Testoni, USA - W.S.M. Werner, Austria - K. Yoshihara, Japan - P.C. Zalm, The Netherlands - A. Zalar, Slovenia Local Organizing Committee - C. Eisenmenger-Sittner, Social Programme - G. Friedbacher, Exhibition/Sponsoring - W. Hofer, IT - H. Hutter, Exhibition/Sponsoring - J. Laimer, Treasurer - M. Marik, Conference Office - F. Ruedenauer, Proceedings - W. Smekal, IT - H. Stoeri, Vice-Chair - W.S.M. Werner, Chair Conference Office Marik Manuela Technische Universitaet Wien Institut fuer Allgemeine Physik / E134 Wiedner Hauptstra§e 8-10 A 1040 Wien Austria Phone: +43 1 58801 13405 Fax: +43 1 58801 13499 e-Mail: ecasia05@iap*tuwien*ac*at For correct e-mail replies please exchange asterisks (*) by dots (.) in all given e-mail addresses ****************************************************************************** From: gord.bramfield@uleth.ca Date: 8 Feb 2005 17:05:39 -0800 Subject: Manuals for Plasmavision /35 Version 3.4 Organization: * Hello. I am looking for manuals that will show how to set up methods to be run on an ARL 34000 (ICP-MS). The software that we are using is Plasmavision /35 version 3.4. Regards, Gord. ****************************************************************************** From: tonyS2@aol.com Date: 8 Feb 2005 21:15:13 -0800 Subject: Hiden mass-spec Organization: * Anybody familar with Hiden mass-spec? What have we got? (Photos) Thanks Tony ****************************************************************************** From: boer Date: Wed, 09 Feb 2005 11:33:47 -0500 Subject: Re: Hiden mass-spec Organization: * tonyS2@aol.com wrote: } Anybody familar with Hiden mass-spec? } What have we got? (Photos) } Thanks } Tony } Your(Photos) was not a link. We use a Hiden analytical turbo-pumped Mass Spectrometer Boer ****************************************************************************** From: tonyS2 Date: 9 Feb 2005 10:06:27 -0800 Subject: Re: Hiden mass-spec Organization: * Boer Re Group email Hiden MSIU Plasma MassSpec with Balzers TCP 305 and Probe control. What is missing? Try site pearlone or TonyS2 on AOL Tony ****************************************************************************** From: Chris Gill Date: Wed, 9 Feb 2005 21:14:53 -0800 Subject: Varian Saturn II External Trigger Organization: * Hello: I have an older Saturn II Ion trap (one of the Varian systems based on the Finnigan ITD/Magnum system - same boards more or less). I need to trigger the MS to begin acquisition from an external contact closure (I want to interface it to a clunky old GC.....). I'm pretty comfortable with modifying electronics if need be, but there must be a "trigger in" somewhere on the system that recognizes a contact closure? If you have done this on a Saturn II, a magnum, an ITD or an ITS-40, it should ~be the same. Any advice or help would be most appreciated.... Cheers, Chris :) **************************************************** Dr. Chris Gill Co-Director Applied Environmental Research Laboratories Department of Chemistry Malaspina University-College Nanaimo, BC, Canada **************************************************** ****************************************************************************** From: Richard Wall Date: Thu, 10 Feb 2005 15:22:27 -0000 Subject: FS Finnigan ITD service manuals Organization: * For Sale set of two service manuals for the Finnigan ITD with circuit diagrams Finnigan p/n 94011-98050 and 94011-98025 Currently in the UK, could ship world wide Contact us via sales@ceinstruments.co.uk Sales of refurbished Carlo Erba/Fisons/Thermo GCs and GC/MS systems ****************************************************************************** From: "[iso-8859-1] Jean-François GAL" Date: Fri, 11 Feb 2005 08:18:57 +0100 Subject: Re: EI ionization cross sections for several gases Organization: * Hi Lutz, I don't know about experimental values for these particular molecules. There is a lot of empirical/theoretical estimation schemes (mostly additive methods) that are more or less reliable. It depends of the required accuracy. Unfortunately most of the additivity schemes are for organics. I can send you some directions if such estimates are useful for your purpose. John Bartmess worked on this matter and may be helpful. To get an idea of the empirical methods, you may read his paper : Bartmess & Giorgiadis, Vacuum, 1983, 33, 149-153. The article is dedicated to ion gauges sensitivities, but this matter is closely related to ionization cross sections, and you may check for cited references. Good luck, Jean-François }Subject: EI ionization cross sections for several gases } }Hello, } }I use a quadrupole with an ionization energy of 77 eV (EI). Can }anybody tell me, where I can find the EI ionization cross sections or }ionization coefficients (probabilities) for the gases/vapors HF(20), }HCN(27), S(32), SO(48), COF2(66), SO3(80), SO2F2(102), SOF4(124) or at }least a few of them? Ionization energies from 70 eV to 100 eV are }acceptable. I already found the EI ionization cross sections for a lot }of molecules and atoms on }"http://physics.nist.gov/PhysRefData/Ionization/", which is avery good }page. }Thanks in advance for your efforts. } }Best regards, } }Lutz ----------------------------------------------------- Prof. J.-F. GAL Laboratoire de Radiochimie, Sciences Analytiques et Environnement (LRSAE) Fac. des Sciences*Parc Valrose Univ. de Nice-Sophia Antipolis 06108 NICE Cedex 2*FRANCE Tel : (33) (0)4 92 07 61 10 Fax : (33) (0)4 92 07 61 11 E-mail : Jean-Francois.Gal@unice.fr (gal@unice.fr) http://www.unice.fr/ ----------------------------------------------------- ****************************************************************************** From: mcksci Date: 11 Feb 2005 13:51:32 -0800 Subject: Mass Specs Organization: * McKinley Scientific have the following mass specs available for sale: Thermo DECA XP Plus Ion Trap Thermo DECA XP Ion Trap Thermo LCQ DECA Ion Trap Thermo LCQ Classic Ion Trap Micromass LCT API-oa Time of Flight Micromass Waters QTOF Ultima Micromass Waters Quattro-LC Micromass Waters Quattro Ultima Micromass Waters ICP Applied Biosystems Mariner ESI-TOF Shimadzu GC17A and QP5000 GCMS Agilent 6890 and Jeol GC Mate II MS Bruker Omniflex MALDI-TOF www.mckscientific.com ****************************************************************************** From: rsiegener@metaenv.com Date: 11 Feb 2005 14:01:59 -0800 Subject: Hydrogen Carrier Gas Organization: * Hi all, We have an agilent 6890/5973 GCMs system with the inert source and ahve been experimenting with Hydrogen as a carrier gas. While we did get a nice 2 minute shift in retention time for PAH amking our run time shorter, we did notice some differences in response for som PAH, particularly drops in response (in terms of both height and area) for anthracene, acenaphthene, benz[a]anthracene, and an increase in response for benzo[e]pyrene. This is on a 60m 0.25mmID, 0.25um film ZB-5. Any suggestions? ****************************************************************************** From: Konradin Stenner Date: Sat, 12 Feb 2005 20:15:24 +0100 Subject: For sale ELAN6000 ICp-MS Organization: * We sell an complete ELAN6000 ICP-MS with computer, Neslab CFT75, and AS91. If you are interested, please contact : ANALYSENTECHNIK STENNER EUROPEAN CETAC SERVICE CENTER HARFFER STR.79 D-41469 NEUSS GERMANY Fon +49 (0)2131 768946 Fax +49 (0)2131 768947 MOBILE +49 (0)179 5117146 mail@stenner.biz www.stenner.biz ****************************************************************************** From: Kendall Date: Mon, 14 Feb 2005 02:29:11 GMT Subject: Re: HELP!! mass spectrometric data conversion Organization: * "Sim" wrote in message news:cu5t9k$cr0$1@news-int.gatech.edu... } Hello I am an Italian resercher and I need help. } } I work with a MALDI (Voyager DE-STR). The spectra are saved in .dat } format. } I would like to convert these data to .mzXML format but as far as I know } it } is not possible to direcly convert the .dat into .mzXML format. Do you } know } if exhist some programs to convert this format to other file formats like } .raw .wiff .cdf? These formats, in fact, can be converted to .mzXML with } some specific tools and this can be a solution to my problems. } } } If you can help me please reply to me both on this newsgroup and using my } E-MAIL: simone.cristoni@virgilio.it } } Thank you very much for your help. } } Simone Try this: http://www.expasy.org/MSight/ Its actually a mass spec. imaging program, however it has a built in utility for conversion of several common file types to mzXML format. I don't know if this will help - but I hope it does! -Kendall ****************************************************************************** From: "rainer [iso-8859-2] lörwald" Date: Tue, 22 Feb 2005 23:17:28 +0100 Subject: Re: Hydrogen Carrier Gas Organization: * Have you checked the pumping capacity of your MS for the different gases? Source pressure and ionisation efficiency may change significantly! Does the Gc know it has to control the flow of hydrogen? Flow rate may also fe affected. rsiegener@metaenv.com schrieb: } } Hi all, } We have an agilent 6890/5973 GCMs system with the inert source and ahve } been experimenting with Hydrogen as a carrier gas. While we did get a } nice 2 minute shift in retention time for PAH amking our run time } shorter, we did notice some differences in response for som PAH, } particularly drops in response (in terms of both height and area) for } anthracene, acenaphthene, benz[a]anthracene, and an increase in } response for benzo[e]pyrene. This is on a 60m 0.25mmID, 0.25um film } ZB-5. Any suggestions? ****************************************************************************** From: "rainer [iso-8859-2] lörwald" Date: Tue, 22 Feb 2005 23:36:35 +0100 Subject: Re: Varian Saturn II External Trigger Organization: * Chris, check, if connecting pins 2 and 3 on the rear panel´s connector starts the acquisition. On ITS40/Magnum these lines are labeled with "ready in" but the pins on SAP PCB are called "start ITD", so there is a small chance of being successful. There is also a "MS ready out" at 7 and 8, NC/NO is selectable on Power Control PCB J102 on original Finnigan machines. I don´t have any schematics of Varian instruments, but the early ones appeared to me to be the same. We never used such a system with any other GC than the Varian 3400. HTH Rainer Chris Gill schrieb: } } Hello: } } I have an older Saturn II Ion trap (one of the Varian systems based on the Finnigan ITD/Magnum system - same boards more or } less). I need to trigger the MS to begin acquisition from an external contact closure (I want to interface it to a clunky old } GC.....). I'm pretty comfortable with modifying electronics if need be, but there must be a "trigger in" somewhere on the } system that recognizes a contact closure? If you have done this on a Saturn II, a magnum, an ITD or an ITS-40, it should ~be } the same. Any advice or help would be most appreciated.... } } Cheers, } } Chris :) } } **************************************************** } Dr. Chris Gill } Co-Director } Applied Environmental Research Laboratories } Department of Chemistry } Malaspina University-College } Nanaimo, BC, Canada } **************************************************** ****************************************************************************** From: Haru79 Date: Thu, 24 Feb 2005 10:16:24 -0500 Subject: HP 5973 GCMSD problem Organization: * Hello there. I would like to ask if anyone could help me out in clarifying the problem with my GCMS machine. Whenever I tried to do an autotune with a higher column flow of He gas; in this case 2.0mL/min, I will obtained a high EMV of above 2200V and above. I tried using a lower column flow(eg 1.8mL/min) and I will obtained a lower EMV of app. 2000V. In both the case, I''m running under constant flow as part of the requirement of the testings being conducted. I would like to ask if this problem arises due to a problematic column or is there any problem with the foreline pump/electron multiplier. I suspected that it''s the column being problematic; ie such as deteriorated column or crushed column due to overtightening column nut..etc..however, I have run a system leak check and there''s no leak in the system. So, I would like to ask if anyone could help me in this and is there any way to prevent me from getting a rather high EMV voltage from autotuning. FYI, I have also cleaned the ion source recently. Furthermore, I believed that the ion source is not the problem due to the fact that I was able to obtain a much lower EMV from lower column flow. Is there any possibilities that it might due to contaminated foreline pump oil or being low in level? I have a more than 90mmTorr foreline pressure. However, this is normal as I've alwyas gotten this pressure with a good tune after ion source cleaning with about 2000EMV; only recently that I have a high autotuning of EMV even after ion source cleaning. I hope someone can help me out and I will really appreciate the help. Thank you~ ****************************************************************************** From: BG Date: Thu, 24 Feb 2005 18:47:27 -0500 Subject: Re: HP 5973 GCMSD problem Organization: * what vacuum pump do you have? The standard turbo pump or the enhanced turbo for the CI option? The observed loss in sensitivity may be due to insufficient pumping capacity at higher flow rates. "Haru79" wrote in message news:cvktdb$1gc$1@news-int.gatech.edu... } Hello there. I would like to ask if anyone could help me out in } clarifying the problem with my GCMS machine. } } Whenever I tried to do an autotune with a higher column flow of He gas; in } this case 2.0mL/min, I will obtained a high EMV of above 2200V and above. } I tried using a lower column flow(eg 1.8mL/min) and I will obtained a } lower EMV of app. 2000V. In both the case, I''m running under constant } flow as part of the requirement of the testings being conducted. } } I would like to ask if this problem arises due to a problematic column or } is there any problem with the foreline pump/electron multiplier. I } suspected that it''s the column being problematic; ie such as deteriorated } column or crushed column due to overtightening column nut..etc..however, I } have run a system leak check and there''s no leak in the system. So, I } would like } to ask if anyone could help me in this and is there any way to prevent me } from getting a rather high EMV voltage from autotuning. } } FYI, I have also cleaned the ion source recently. Furthermore, I believed } that the ion source is not the problem due to the fact that I was able to } obtain a much lower EMV from lower column flow. } Is there any possibilities that it might due to contaminated foreline pump } oil or being low in level? I have a more than 90mmTorr foreline pressure. } However, this is normal as I've alwyas gotten this pressure with a good } tune after ion source cleaning with about 2000EMV; only recently that I } have a high autotuning of EMV even after ion source cleaning. } } I hope someone can help me out and I will really appreciate the help. } Thank you~ } } ****************************************************************************** From: Haru79 Date: Sat, 26 Feb 2005 03:32:38 -0500 Subject: Re: Re: HP 5973 GCMSD problem Organization: * Hi there.My foreline pump is not a turbo type and I ran it under EI mode. Its one by Inland Vacuum Systems. Insufficient pumping capacity?What does that mean?Are you saying my pump was not operating effectively?FYI, previously, I ran it under the same high flowrate and everything is fine. Just recently, this problem arises. Do advice more. Thanks~ ****************************************************************************** From: BG Date: Sat, 26 Feb 2005 12:28:09 -0500 Subject: Re: Re: HP 5973 GCMSD problem Organization: * OK...The foreline pump is not the high vacuum pump. It is the low vacuum pump (mTorr range) that is connected to the high vacuum pump on the vacuum manifold (i.e. turbo pump, 10^-5 to 10^-6 Torr). If you do not have CI capability on that mass spec (and the higher capacity turbo), then it may be a safe assumption that you have the standard turbo pump. While Agilent states that the standard turbo can handle up to 2 ml/min of helium flow through the column, the sensitivity will be much lower than at lower helium flow rates. At higher helium flows the pressure in the vacuum manifold increases and the mean free path of the ions therefore decreases, i.e. fewer ions reach the detector and the autotune increases the EMV voltage in order to get the ion current it is looking for. If you have an ionization guage controller to measure the vacuum in the mass spec and it reads in the mid to high 10^-5 torr range, then your pumping capacity is not ideal and lower sensitivity is observed. You originally said that the you normally ran the mass spec with 2mL/min helium flow. Had you ever previously tuned it with 1.8 mL/min helium flow before this "problem" was discovered? If there is no previous benchmark between 1.8 and 2.0 mL/min flow, then how do you know there is a problem? Also, if you have a diffusion pump instead of a turbo pump, then all bets are off. The diffusion pumps they sell with these definitely do not have sufficient pumping capacity. I really can't help more without knowing the specifics of the instrument and it's history. Bill "Haru79" wrote in message news:cvpo49$et8$1@news-int.gatech.edu... } Hi there.My foreline pump is not a turbo type and I ran it under EI mode. } Its one by Inland Vacuum Systems. } } Insufficient pumping capacity?What does that mean?Are you saying my pump } was not operating effectively?FYI, previously, I ran it under the same } high flowrate and everything is fine. Just recently, this problem arises. } } Do advice more. } } Thanks~ } } ****************************************************************************** From: jduan111@yahoo.com Date: 28 Feb 2005 11:49:42 -0800 Subject: mass accuracy problem Organization: * hello, there, I just wonder whether there may be some expert there who can help me solve the following problem. I met one question about the mass accuracy on the MicroMass LCT. I took spectra on Micromass LCT for three small molecules one by one continuously. The three experiments were finished in 10 minutes. However, I got totally different accuracy on three molecules: First one: mass accuracy 17ppm with Molecular weight 416.18 Second one: mass accuracy 161ppm, with Molecular weight 474.17 Third one: mass accuracy 441ppm, with Molecular weight 500.08 The physical properties of three molecules are very different, such as polarity, molecular structures are pretty different too. I just want to consult experts out there whether the molecular properties will cause so much difference in accuracy. I do not feel temperature flucturated so much in ten minutes and I can not imagine voltage will fluctuate so much either. Thanks so much!!! jin ****************************************************************************** From: Kendall Date: Tue, 01 Mar 2005 01:55:22 GMT Subject: Re: mass accuracy problem Organization: * wrote in message news:d0073g$8bm$1@news-int2.gatech.edu... } } hello, there, I just wonder whether there may be some expert there who } can help me solve the following problem. } } I met one question about the mass accuracy on the MicroMass LCT. I } took spectra on Micromass LCT for three small molecules one by one } continuously. The three experiments were finished in 10 minutes. } However, I got totally different accuracy on three molecules: } } First one: mass accuracy 17ppm with Molecular weight 416.18 } Second one: mass accuracy 161ppm, with Molecular weight 474.17 } Third one: mass accuracy 441ppm, with Molecular weight 500.08 } } The physical properties of three molecules are very different, such as } polarity, molecular structures are pretty different too. } } I just want to consult experts out there whether the molecular } properties will cause so much difference in accuracy. I do not feel } temperature flucturated so much in ten minutes and I can not imagine } voltage will fluctuate so much either. } } Thanks so much!!! } } jin No, molecular properties should *not* influence the accurate mass measurements. You know, even the "good" measurement of the three you reported (17ppm) is pretty terrible for a TOF - I don't know the specs of your machine, but I would think it should be able to produce <10ppm on a regular basis.. Some things to try/think about: 1. TOF instruments sometimes take a while to "warm up" after being switched on - was your instrument on and equilibrated? 2. Did you perform a mass calibration just before taking the measurements? You should try that, if you didn't already. 3. You may want to consider introducing your analyte in the presence of a mass calibrant (i.e. "lock mass") so that potential instrument fluctuations can be ruled out (only after #1 and #2 have been done, though). -KP ****************************************************************************** From: cody@jeol.com Date: 2 Mar 2005 04:17:51 -0800 Subject: Re: mass accuracy problem Organization: * Your problem with mass accuracy is unrelated to structure. Two thoughts come to mind: 1. At the ppm-level mass accuracies that you should be getting from a TOF, you should be getting error in the 3rd or 4th decimal place. You will need to make use of more than two decimal places to determine your error. 2. Although you should be using a lock mass to achieve reliable accuracy, such a drastic difference in mass accuracy in a short time is not related to drift. Is this an older-model LCT? If so, you will need to inject a very dilute sample. Your difference in mass accuracy is probably more related to concentration than anything else. The TDC detector in the older LCTs is an ion-counting detector can easily saturate at higher concentrations, leading to a concentration-dependent error. (That's why we introduced our own ADC-based model a few years ago to avoid this problem.) Try diluting your samples and reinjecting them. You should see an improvement as you get closer to the detection limits. There are mathematical approaches that are supposed to deal with concentration-dependent response on the LCT. See what your manuals have to say about statistical corrections, but try diluting the samples first. 3. The other posting is correct -- you should be getting errors on the order of 5 ppm if everything is working right. Chip Cody (JEOL USA, Inc.) jduan111@yahoo.com wrote: } hello, there, I just wonder whether there may be some expert there who } can help me solve the following problem. } } I met one question about the mass accuracy on the MicroMass LCT. I } took spectra on Micromass LCT for three small molecules one by one } continuously. The three experiments were finished in 10 minutes. } However, I got totally different accuracy on three molecules: } } First one: mass accuracy 17ppm with Molecular weight 416.18 } Second one: mass accuracy 161ppm, with Molecular weight 474.17 } Third one: mass accuracy 441ppm, with Molecular weight 500.08 } } The physical properties of three molecules are very different, such as } polarity, molecular structures are pretty different too. } } I just want to consult experts out there whether the molecular } properties will cause so much difference in accuracy. I do not feel } temperature flucturated so much in ten minutes and I can not imagine } voltage will fluctuate so much either. } } Thanks so much!!! } } jin ****************************************************************************** From: Hubert Tang Date: Wed, 02 Mar 2005 15:08:27 +0000 Subject: Re: HP 5973 GCMSD problem Organization: * The EMV value in AUTOTUNE depends very much on the vacuum condition in the MSD. As vacuum becomes lower due to high column flow rate, less charged ions are allowed to pass through the quadrupole due to reduction of mean free path. As such, the detector, i.e the electron multiplier has to increase its EMV in order to produce sufficient abundance (normally preset at about 500,000) of m/z 69. That's the reason why high EMV is usually observed when high column flow rate is used. For 5973MSD, the manufacturer claims that the MSD can sustain 4mL/min, 2mL/min, and 1mL/min for performance turbo, standard turbo, and diffusion pump, respectively. However, in my opinion, never excess 2mL/min of column flow rate even a performance turbo pump is installed as sensitivity may drop. Hubert >sabrestormeagle@hotmail.com> wrote >Hello there. I would like to ask if anyone could help me out in >clarifying the problem with my GCMS machine. > >Whenever I tried to do an autotune with a higher column flow of He gas; in >this case 2.0mL/min, I will obtained a high EMV of above 2200V and above. >I tried using a lower column flow(eg 1.8mL/min) and I will obtained a >lower EMV of app. 2000V. In both the case, I''m running under constant >flow as part of the requirement of the testings being conducted. > >I would like to ask if this problem arises due to a problematic column or >is there any problem with the foreline pump/electron multiplier. I >suspected that it''s the column being problematic; ie such as deteriorated >column or crushed column due to overtightening column nut..etc..however, I >have run a system leak check and there''s no leak in the system. So, I would like >to ask if anyone could help me in this and is there any way to prevent me >from getting a rather high EMV voltage from autotuning. > >FYI, I have also cleaned the ion source recently. Furthermore, I believed >that the ion source is not the problem due to the fact that I was able to >obtain a much lower EMV from lower column flow. >Is there any possibilities that it might due to contaminated foreline pump >oil or being low in level? I have a more than 90mmTorr foreline pressure. >However, this is normal as I've alwyas gotten this pressure with a good >tune after ion source cleaning with about 2000EMV; only recently that I >have a high autotuning of EMV even after ion source cleaning. > >I hope someone can help me out and I will really appreciate the help. >Thank you~ ****************************************************************************** From: Mike Sherrell Date: Wed, 2 Mar 2005 08:52:23 -0800 Subject: Mass specs and MALDIs available Organization: * Mass specs and MALDIs available from Grizzly Analytical: **LC/MS & MS/MS: API QSTAR^Ù Pulsar i: $225,000. 2002. Hybrid w/ oMALDI. Installed, guaranteed ok for svc. Q-Star Pulsar: $170,000. Not the "i". Recently pmd. + $16K/factory install. Q-Star Pulsar XL: Call/email to discuss price. Hybrid Q-TOF. Sciex API 4000: $265,000. Running in lab now; can be tested. API 3000 upgrade: $38,500. Increases sensitivity and (S/N) Ratio at high flow rates to ~ that of an API 4000. Install incl. Sciex API 2000: $70,000 installed and warranteed. Sciex API 2000: $120,000: Incl. EPQ3 upgrade to ~ API 3000 sensitivity, install & Warranty. Sciex API 2000 upgrade: $25,000. 4x sensitivity increase; incl. install, warr. Sciex API 365: $65,000. NT, turboionspray. Installed, warranteed. Sciex 365 w/ EP10+: $149,000. Custom upgrade; more sensitive than API 3000. Sciex API 365 upgrade: $109,000. 10x+ sensitivity upgrade; near-equal to 4000. Sciex API 150EX: $48,000. MCA upgraded to EX; identical performance. MAC; NT + $10,000. Incl. install. Sciex NT workstation: $2,500. Use to upgrade Macs on 150, 365, 2000, 3000. Sciex API III+: $25,000. Triple quad: ES, APCI; +$24K/intall w/ 1 yr. warr. Sciex API III+ wanted for tax-deductible donation to a university. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $20,000/installation and 1 year service contract. Sciex Turboionspray source (aka ESI) for API 150, 365 or 3000: $6,250. Sciex MicroIon spray source: $7,900. For API 150, 300 or Q-Star. Very low flow. PE-ABI Mariner: