****************************************************************************** From: "M Sweeney - MSMS Consulting" Date: Wed, 02 Jan 2002 21:30:25 GMT Subject: LCQ - modified german versions? Organization: EarthLink Inc. -- http://www.EarthLink.net Open question. I was asked about the ramifications on ion-trap operations of the german court ruling cited at http://www.thermo.com/eThermo/CDA/News/News_Detail/0,1247,10616-113,00.html It mentions modifications, but I am not clear what they are or how they change function if at all. Comments? -- Matt Sweeney mattsweeney@earthlink.net Mass Spec Consulting Training/Operations/Consulting/Method Development LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism, Maintenance Classes, Specialist in Finnigan Equipment and Software ****************************************************************************** From: "Anon" Date: Thu, 3 Jan 2002 19:42:48 -0000 Subject: Re: ACN gradient fouls Source Organization: ntlworld News Service This problem relates to choice of acetonitrile as solvent in APCI a "carbon whisker" can buildup on the corona needle due to acetonitrile. The only solution is to either replace acetonitrile with something like methanol or reduce corona discharge voltage to minimise problem "Chris Vlahakis" wrote in message news:9vtd66$1qk$1@news-int.gatech.edu... } "Benning, Matthew" wrote: } }}I am having problems with acetonitrile gradients (0.25 ml/min) using an LCQ }}APCI ion source in that the heated capillary quickly gets covered in 'soot'. }}The corona discharge needle also exhibits periodic fouling. The samples are }}lipids and esters (5 ul, 0.1%) that when analyzed with an IPA gradient do }}not exhibit the fouling. The source is periodically baked according to the }}Finnigan directions so I do not believe this is the problem. Any }}suggestions? Thanks in advance. }}Matt B. } } } I have experienced "soot" on the end of the heated capillary also } using acetonitrile with APCI on an LCQ. I try to keep it clean by } removing with a kimwipe saturated with MeOH. Then use the "Reamer" to } clean out the heated capillary. } } Hope that helps. } } Chris } Chris Vlahakis } } ****************************************************************************** From: Giovanna.Tripepi@bruker.it (Giovanna Tripepi) Date: Sat, 5 Jan 2002 10:01:25 +0100 Subject: Occasion: MALDI-TOF for sale Organization: * Bruker Daltonics S.r.l. in Italy has an extremely well performing Biflex = III with Reflectron and FAST two years old, always used in research = environment, available for sale at very convenient conditions. If anyone is interested in it, please contact me at: giovanna.tripepi@bdal.it tel. +39 - 02 - 70 63 63 70 Best regards, Giovanna ****************************************************************************** From: Daniel Boismenu Date: Sat, 05 Jan 2002 20:35:39 GMT Subject: Re: ACN gradient fouls Source Organization: * Greetings Chris, When having problem of 'soot', most people suspect their organic solvents right away. ****************************************************************************** From: Daniel Boismenu Subject: Re: ACN gradient fouls Source Date: Sat, 05 Jan 2002 20:35:39 GMT Organization: * It is during a conversation with Dr Ann English from Concordia University that she pointed out that if you have 18 Mohms of resistivity for your filtered water, that only means that there is no ions. You could have several mg of amorphous carbon per liter leaching out of the activated carbon cartridge of your filtering system. These particles of amorphous carbon are coated with all kinds of organic compounds, such as PAH and will get trapped at the head of your HPLC column. When you run the acetonitrile gradient, the ACN desorbs the compounds from the particles and they are sprayed in the source resulting in getting it dirty. As soon as we changed from cartridge filtered water to HPLC grade in glass distilled water, we corrected the problem. With this water, last time we cleaned the sampling cone was 3 months ago and it is still looking good, while with cartridge filtered water, we had to clean it each week. Best regards, Daniel Boismenu, Ph.D. Montréal Network for Pharmaco-Proteomics and Structural Genomics } } } "Chris Vlahakis" wrote in message } news:9vtd66$1qk$1@news-int.gatech.edu... } } "Benning, Matthew" wrote: } } } }}I am having problems with acetonitrile gradients (0.25 ml/min) using an LCQ } }}APCI ion source in that the heated capillary quickly gets covered in 'soot'. } }}The corona discharge needle also exhibits periodic fouling. The samples are } }}lipids and esters (5 ul, 0.1%) that when analyzed with an IPA gradient do } }}not exhibit the fouling. The source is periodically baked according to the } }}Finnigan directions so I do not believe this is the problem. Any } }}suggestions? Thanks in advance. } }}Matt B. } } } } } } I have experienced "soot" on the end of the heated capillary also } } using acetonitrile with APCI on an LCQ. I try to keep it clean by } } removing with a kimwipe saturated with MeOH. Then use the "Reamer" to } } clean out the heated capillary. } } } } Hope that helps. } } } } Chris } } Chris Vlahakis } } } } ****************************************************************************** From: Cameron Dorey Date: Mon, 07 Jan 2002 09:36:32 -0600 Subject: Re: ACN gradient fouls Source Organization: University of Arkansas at Little Rock Chris Vlahakis wrote: } } "Benning, Matthew" wrote: } } }I am having problems with acetonitrile gradients (0.25 ml/min) using an LCQ } }APCI ion source in that the heated capillary quickly gets covered in 'soot'. } }The corona discharge needle also exhibits periodic fouling. The samples are } }lipids and esters (5 ul, 0.1%) that when analyzed with an IPA gradient do } }not exhibit the fouling. The source is periodically baked according to the } }Finnigan directions so I do not believe this is the problem. Any } }suggestions? Thanks in advance. } }Matt B. } } I have experienced "soot" on the end of the heated capillary also } using acetonitrile with APCI on an LCQ. I try to keep it clean by } removing with a kimwipe saturated with MeOH. Then use the "Reamer" to } clean out the heated capillary. Haven't used ES or APCI, but the soot problem just reminds me of the old qual test for unsaturates. If, upon burning, you got soot, there was significant unsaturated character to the compound, presumably because the C-H balance was upset. Helped me ID 1-aminonaphthalene real quickly in a grad course many moons ago. Cameron -- Cameron Dorey Associate Professor of Chemistry University of Central Arkansas Phone: 501-450-5938 camerond@mail.uca.edu ****************************************************************************** From: "a.p.bruins" Date: Mon, 7 Jan 2002 17:50:35 +0100 Subject: Re: ACN gradient fouls Source Organization: * The formation of carbon deposits on the corona needle might be avoided by the use of air as nebulizer gas. Corona discharges are generally more stable if oxygen is present. Andries Bruins "Anon" wrote: }This problem relates to choice of acetonitrile as solvent in APCI a }"carbon whisker" can buildup on the corona needle due to }acetonitrile. The only solution is to either replace acetonitrile with }something like methanol or reduce corona discharge voltage to minimise }problem }"Chris Vlahakis" wrote in message }news:9vtd66$1qk$1@news-int.gatech.edu... }} "Benning, Matthew" wrote: }} }}}I am having problems with acetonitrile gradients (0.25 ml/min) using an }}}LCQ APCI ion source in that the heated capillary quickly gets covered }}}in 'soot'. The corona discharge needle also exhibits periodic }}}fouling. The samples are lipids and esters (5 ul, 0.1%) that when }}}analyzed with an IPA gradient do not exhibit the fouling. The source }}}is periodically baked according to the Finnigan directions so I do not }}}believe this is the problem. Any suggestions? Thanks in advance. }}}Matt B. }} }} }} I have experienced "soot" on the end of the heated capillary also }} using acetonitrile with APCI on an LCQ. I try to keep it clean by }} removing with a kimwipe saturated with MeOH. Then use the "Reamer" to }} clean out the heated capillary. }} }} Hope that helps. }} }} Chris }} Chris Vlahakis -- Dr. A.P. Bruins University of Groningen Mass Spectrometry Core Facility Centre for Pharmacy A. Deusinglaan 1 9713 AV Groningen The Netherlands tel +31-50-3633262 fax +31-50-3638347 a.p.bruins@farm.rug.nl ****************************************************************************** From: Drew Gibson Date: Mon, 7 Jan 2002 22:01:58 +0000 Subject: Re: ACN gradient fouls Source Organization: is sadly lacking a.p.bruins wrote ... }The formation of carbon deposits on the corona needle might be }avoided by the use of air as nebulizer gas. Corona discharges are }generally more stable if oxygen is present. Sciex always used to warn against this - solvent + high temp + oxygen + corona discharge = potential explosion. I heard a story about an API 3 which lost its source across the room after such a bang, don't know if it's true or not (maybe the user was not using the exhaust correctly ?) but it may pay to be careful here. Drew, wishing I was going to Sanibel at the end of the month ! -- Cheers, >>> mailto:drew@thegibsons.demon.co.uk <<< }}} ICQ 392790 IRC Gibby <<< Drew ;^) >>> http://www.thegibsons.demon.co.uk <<< ****************************************************************************** From: jsherratt@vrs-uk.net (John Sherratt) Date: 8 Jan 2002 09:41:40 -0800 Subject: Application Chemist Opportunity, West Midlands, UK Organization: http://groups.google.com/ Further expansion at this leading manufacturer of Mass Spectrometry instruments and accessories for the Biotech and Life Science industries. They are keen to recruit Applications Chemists to join the busy applications department. This is a very diverse role that incorporates activities such as customer demonstrations, sample running, training, application troubleshooting and instrument maintenance. A sound understanding of mass spectrometry is essential for this role, though specific product training will be provided. Experience and knowledge of MALDI applications would be ideal and knowledge of proteomics advantageous. Good and confident communication skills are also essential, along with the ability to think on your feet and work to deadlines. This is an excellent opportunity to work with a global leader in its field in a highly diverse role with the latest mass spectrometry technology. For further information, please contact John Sherratt on: +44 161 976 4000 or email jsherratt@vrs-uk.net VRS, Chapel House, 59 Chapel Road, Sale, Cheshire, M33 7FD ****************************************************************************** From: "James L. Loo" Date: Tue, 8 Jan 2002 15:55:29 -0800 Subject: ESI vs Pregnancy Organization: * I have been asked if the RF energy emanating from our QUATTRO II ESI Quadrupole Analyzer poses any potential pregnancy hazards. Is there a good reference on this subject? James Loo NMR & Mass Spec Facility Manager UCSC Santa Cruz, CA 95064 -- ====================_/=================================================== _/_/_/ _/_/_/ As Zeus said to Narcissus, "Watch Yourself" _/ _/ loo@chemistry.ucsc.edu _/ _/ http://www.nmr.ucsc.edu _/ _/ (831) 459-2485 Office _/ _/ _/ (831) 459-4923 Lab _/ _/ _/_/_/_/_/ (831) 459-2935 Fax = _/_/_/_/============================================================== ****************************************************************************** From: "Dave White" Date: Tue, 8 Jan 2002 18:21:02 -0600 Subject: Re: ESI vs Pregnancy Organization: VISI.com The Quattro's are pretty well shielded, I reckon you'd get more RF exposure from a cell phone than from working on a Quattro. Just my non scientific 2 cents worth. "James L. Loo" wrote in message news:a1g1d0$8dn$1@news-int.gatech.edu... } I have been asked if the RF energy emanating from our QUATTRO II ESI } Quadrupole Analyzer poses any potential pregnancy hazards. Is there } a good reference on this subject? } ****************************************************************************** From: Sarah Shealy Date: Tue, 08 Jan 2002 19:48:37 -0500 Subject: Re: ESI vs Pregnancy Organization: * The FCC has a FAQ at http://www.fcc.gov/oet/rfsafety/rf-faqs.html about the safety of RF emission. ****************************************************************************** From: "James Barnett" Date: Wed, 9 Jan 2002 01:05:50 -0800 Subject: Re: ESI vs Pregnancy Organization: * Hi, In your case in the USA, check out the FCC web site, www.fcc.gov I guess the equipment will have an FCC ID like most electronic equipment in the USA or CE mark in Europe, so it would have been tested to comply with local regulations on ionizing radaition.. Theres most likely as test certicate available for the equipement with the manufacture.. any how you can read this pdf file, if you want to get in depth or do a search for more stuff. http://www.fcc.gov/Bureaus/Engineering_Technology/Orders/1996/fcc96326.pdf The rf energy would be extremely low, compared to cell phones ect. as the metal case would act like a Faraday cage, put simply rf enegy can gt out of screened box at 0V. its really nothing to worry about. Regards, Jim "James L. Loo" wrote in message news:a1g1d0$8dn$1@news-int.gatech.edu... } I have been asked if the RF energy emanating from our QUATTRO II ESI } Quadrupole Analyzer poses any potential pregnancy hazards. Is there } a good reference on this subject? } } } James Loo } NMR & Mass Spec Facility Manager } UCSC } Santa Cruz, CA 95064 } -- } } } } } } ====================_/=================================================== } _/_/_/ _/_/_/ As Zeus said to Narcissus, "Watch Yourself" } _/ _/ loo@chemistry.ucsc.edu } _/ _/ http://www.nmr.ucsc.edu } _/ _/ (831) 459-2485 Office } _/ _/ _/ (831) 459-4923 Lab } _/ _/ _/_/_/_/_/ (831) 459-2935 Fax } = _/_/_/_/============================================================== } } ****************************************************************************** From: "Simon" Date: Wed, 9 Jan 2002 14:10:32 +0100 Subject: MALDI and Virtual 2D Organization: Universita' degli Studi di Milano Good day, I am a Biochemical researcher working at the University of Milan. I study protein and peptides molecules using mass spectrometry (ESI and MALDI). I have recently read the follow paper: Rachel R. Ogorzalek Loo,*,?,? James D. Cavalcoli,§ Ruth A. VanBogelen,§ Charles Mitchell,?,| Joseph A. Loo,§ Brian Moldover,§,^ and Philip C. Andrews*,? "Virtual 2-D Gel Electrophoresis: Visualization and Analysis of the E. coliProteome by Mass Spectrometry" Anal. Chem.2001, 73,4063-4070 In this paper the second dimension of classical 2D-GEL has been sobstitued with the MALDI technique. My questions are: 1) In the paper it is specified that the MALDI (PerSeptive-Vestec Voyager Elite and PerSeptive) use the LINEAR MODE in order to perform the analysis on the first dimension of the GEL. Do you know what does LINEAR MODE means? 2) Do you know some laboratory that are able to use this technique? I am strongly interested in collaboration with this groups. Plese reply to me at the follow E-MAIL address: dtoycr@tin.it Thank you very much for your help. Simone ****************************************************************************** From: "Mike Sherrell" Date: Wed, 9 Jan 2002 11:47:34 -0800 Subject: LC/Mass specs and MALDIs for sale Organization: * LC/Mass specs and MALDIs for sale: *Mass specs: Micromass Ultima: $220,000. Asking price. (This instrument is not the Autospec) Sciex API 3000: $160,000. With Mac; price includes install and 30-day warr. +$7K/NT data system upgrade Sciex API 365: $55,000(!) Guaranteed good working order. 10x sensitivity upgrade: $99,000. For API 365 or 300. Performed on-site; 1 year warr. Sciex API 150: $49,000. Includes E. Coast install HP 1100 MSD: $55,000. Model A; esi + APCI. Bruker Esquire: $85,000. Includes ship, install, HP 1100. Ion trap; 1.5 years old. Finnigan LCQ Classic: $85,000. API, APCI, installed, 30-day warranty Sciex API III+: $39,000. Triple quad: ES, APCI; +$15K/intall w/ 90-day warr. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $15,000/installation and 1 year service contract. Micromass LCZ Pltfrm: $50,000. 2.5 yrs old; ESI, APCI; +$5K/install MicroMass Quattro II: $195,000. Price includes installation & site license Finnigan Navigator: $42,500. Guaranteed good working order Finnigan SSQ 7000: $40,000. ES, APCI; Excalibur; API 1 source; install included Finnigan TSQ 700: $25,000. Electrospray, APCI, Alpha workstn. ~$3K/install Finnigan MAT 900: $75,000. Offers considered. EI, ES, APCI. MAT 95: $260,000. Hi res mag sector. 1994, ES, APCI, FAB, Thermospray, GC interface, install, delivery, 1 yr warr. Finnigan MAT 90: $15,000. Hi-resolution magnetic sector AMD 604 S: $150,000. Double-focusing mag sector; very low detection limits, <4ppm mass, ESI/EI and FD sources. Install/service available. Offers condered. Kratos Concept: $5,000. Plus crating/shipping. Hi res mag sector, ~1991, loaded. VG70E-HF: $20,000. Hi-res mag sector; EI/CI, FAB. Recent refurb. VG70-250SE: $12,500. Does not include SIOS/workstation. Many probes, many parts Xtrell 400 ELQ: $35,000. Or best offer. LC/MS/MS, GC/MS/MS; 10. yrs. old, floor model, well-maintained Fisons VG 2000: <$100,000 Fisons VG Trio: $25,000. LC + GC: 3000. amu; thermospray, EI/CI, HP 5890 included. Install, license & 90-day warr. +: $14,500 HP 5989: $30,000. Electrospray; 2000. amu. +$20,000/HP 1090. w/ ternery pump, autosampler, DAD, install and 90-day warr. VG Trio 2: $7,500. Electrospray; complete; parts or fixer-upper unit. Nermag R10 10c <$10,000. Like new; make offer VG 7070-EHF small $s. 1984 model; used for FAB. Service and service contracts available for PESciex API 3000, 365 and III+. *MALDI-TOFs: Micromass Q-TOF 1: $170,000. Incl. Waters Micro LC. Installation/warranty available. Voyager DE RP: $85,000. Factory rebuilt. Voyager DE Linear: $69,000. 2 yrs old. 2 gHz data acqstn system. Can be tested. Bruker Biflex III: ~$50,000. DE; decommisioned by Bruker. LaserTec II: $75,000. By PerSeptive. 5 yrs. old; excellent condition. SRI custom design: $100,000 asking price. 384 samples/20 min. Can be tested. Voyager LBT2: $20,000. ~1993. Excellent condition. Non-DE. Finnigan MAT Vision 2000. Reflectron: $80,000. Includes install, 1 year warranty Thermo/Finnigan Dynamo linear DE: $39,000. Includes West Coast install, 90-day warranty LaserMAT 2000: $24,000. New detector & laser; +$2,000/install; service available VG-TofSpec: $5,000 or best offer. Disfunctional but complete, new laser card and other boards. *Other mass specs, NMRs, DNA/protein sequencers & synthesizers available; check the website. All listed items subject to prior sale. Regards, Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: alex.buko@abbott.com Date: Thu, 10 Jan 2002 13:06:36 -0600 Subject: Re: M+56 adduct in ESI LC-MS Organization: * The note below from Andrew is apparently an old note dated back in November, but for what it is worth, I have some input. Assumption: The compound is validated so the correct MW is 200 da. Question: If the mass peak observed is really 256 and not 255 then the options are very limited. The odd mass peak would suggest either a transition metal adduct or a nitrogen containing adduct. In either case a single charge must be accounted for. Metal adduct-there have been suggestions that this might be an iron adduct. Not at all likely. Any dissolvable iron ion would most likely in the ferrous state (+2) or less likely the ferris state (+3). The ferrous ion adduct to a molecule of MW 200 would have a double charge mass peak at 200 + 56 / 2 or 128. Proton abstraction from the alcohol substrate to produce a single negatively charged parent ion (199) paired with a double charged ferrous ion (56) would yield a single charge adduct at mass peak 255. If this was the case, check you mass assignment and look for the tell tale iron isotopes as one author suggested. If suspected, try adding lowering the pH or adding base to the flow and this may dislodge the metal. I doubt this is the case. Nitrogen bearing adduct - An ammonium chelate is always possible, but that would be an 18 adduct. I suppose a potassium adduct (39+) with one molecule of ammonia (17) would up, but doesn't seem at all likely. Question: Could the mass assignment be 255 instead of 256? If so then a few things are possible. It has already been suggested that the sodium adduct (+23) to a neutral methanol adduct (32) would add up to 255, but then I would not necessarily expect the thing to fragment under MS/MS, plus I don't see a driving force for such a thing. Try using deuterated methanol and see if the mass shifts. By the way the fragment at 183 is very normal. One author suggested loss of ammonia, but if the protonated molecular ion is 201 then loss of water (18) from 201 would yield a mass peak at 183. For an alcohol compound this would be expected. The other option is addition of a protonated water complex. One of the magic coordination numbers for water is 3,thus three water molecules clustered together has a mass of 54, add on proton and you get a single charge adduct of mass 55. I believe we have seen these with arginine containing peptides. The particular compound may be able to chelate or coordinate with a protonated water cluster using atmospheric pressure electro spray ionization, assuming 255 not 256. Good luck - Alex Buko }I'm a newbie to LC-MS, so please excuse me if this is a "no brainer" }question.... } }We are developing a method to analyse 3-phenoxybenzyl alcohol (aka }3-phenoxybenzenemethanol) by LC-MS on a ThermoFinnigan LCQ Duo using ESI. }We are infusing a neat standard solution directly into the source via a }T-piece and a mobile phase of 70% MeOH and 30% H2O at 500 uL/min. We get }a very strong peak at [M+56]+ and only a weak peak at [M+H]+ (M = 200.2 }for 3-PBA). Which adduct does M+56 represent? When we do an MS/MS }experiment on the [M+56]+ ion we do see the product ion at m/z 183.1 as }predicted for [M+H]+ by fragmentation software. Also, this adduct is }formed when we are using either acetic acid or ammonium acetate as mobile }phase modifiers. We do not see this adduct with any of the other }compounds which we are looking at so I assume it to be compound specific }(ie. not a contaminant in the system). } }Does anyone know of any compilations (ie. tables, lists, etc.) of common }and not-so-common adducts which are encountered in LC-MS? } }Andrew } }=========================================== }Dr. Andrew J. Peters }Natural Environment Research Council }Centre for Ecology and Hydrology }Chemical and Molecular Ecology Section }Winfrith Technology Centre }DORCHESTER, Dorset, DT2 8ZD, United Kingdom }e-mail: anpe@ceh.ac.uk }Tel: +44 (0)1305 213631 Fax: +44 (0)1305 213600 ****************************************************************************** From: markus.rueckert@morphochem.de (mrueckert) Date: 10 Jan 2002 23:50:39 -0800 Subject: Associate Bio-Analytical Scientist Position Organization: http://groups.google.com/ Morphochem is a rapidly-expanding drug discovery company which successfully employs state-of-the-art biotechnology tools to identify new classes of small molecule therapeutics. We have facilities in Munich (Germany, Headquarters), Basle (Switzerland) and Princeton (U.S.A.). We are currently looking for an experienced bio-analytical scientist to join our team in Munich. Description: Your responsibilities will include the development and validation of analytical methods based on LC-MS/MS for the quantitative determination of drug-candidates and their metabolites in biological specimens and the application of these methods in support of pharmacokinetic studies in preclinic drug discovery programs. Responsibilities: You will conduct experiments that will be coordinated within a multidisciplinary team to identify and prioritize new drug candidates. You will determine the pharmacokinetic and metabolic characteristics of new compounds in a very early stage of the pre-clinical drug discovery process. You will perform all stages of method development with a triple quadrupole LC-MS/MS-system. Relevant Working Experience: As an ideal candidate you have a strong background in analytical chemistry with a focus on HPLC, mass spectrometry and LC-MS instrumentation with at least one year experience in the pharmaceutical industry. A working knowledge of complex sample preparation, extraction from biological matrices and quantitation of small molecules in body fluids with LC-MS/MS is essential. Strong interpersonal and excellent oral/written communication skills are required. Strong computer skills, the ability to work with others, attention to detail, and the ability to think critically is desired. Applicants should be highly motivated and capable of working independently in a dynamic environment. Experience with sciex instrumentation and software would be a plus. German language skills would be helpful but are not required. Education: This position requires a BS or MS or comparable degree in analytical, pharmaceutical, organic chemistry, or other closely related disciplines, with clear evidence of academic excellence. If you enjoy working as part of a team in a dynamic and challenging environment where initiative and flexibility are rewarded, then do not hesitate to contact us. Our employment package includes a competitive salary, attractive stock option plan and relocation expenses. To apply submit resume to: markus.rueckert@morphochem.de www.morpchochem.com ****************************************************************************** From: "George Antoniades" Date: Fri, 11 Jan 2002 13:21:24 GMT Subject: Re: ESI vs Pregnancy Organization: BigPond Internet Services (http://www.bigpond.net.au) What's the instrument serial number? "James L. Loo" wrote in message news:a1g1d0$8dn$1@news-int.gatech.edu... } I have been asked if the RF energy emanating from our QUATTRO II ESI } Quadrupole Analyzer poses any potential pregnancy hazards. Is there } a good reference on this subject? } } } James Loo } NMR & Mass Spec Facility Manager } UCSC } Santa Cruz, CA 95064 } -- } } } } } } ====================_/=================================================== } _/_/_/ _/_/_/ As Zeus said to Narcissus, "Watch Yourself" } _/ _/ loo@chemistry.ucsc.edu } _/ _/ http://www.nmr.ucsc.edu } _/ _/ (831) 459-2485 Office } _/ _/ _/ (831) 459-4923 Lab } _/ _/ _/_/_/_/_/ (831) 459-2935 Fax } = _/_/_/_/============================================================== } } ****************************************************************************** From: "Antony Williams" Date: Fri, 11 Jan 2002 11:33:36 -0500 Subject: Position Opening in Europe Organization: * ChemCAD (www.chemcad.fr) is expanding its reach to provide analytical chemistry software to the Netherlands and the Flemish part of Belgium. We are actively seeking a Dutch speaking addition to our team who will function as a scientific liaison with our Dutch speaking clients. Specifically this new person will be charged with driving the commercial success of Advanced Chemistry Development's software products throughout the Netherlands and the Flemish part of Belgium. The new person is also expected to coordinate training throughout this region. The successful candidate will be a highly motivated self-starter who has the intellectual agility to switch between sales, support and training roles. Because the products are highly specialized it is vital that the candidate have direct experience with NMR, MS or HPLC (all three is ideal). Past success in new business development and customer retention in this field of science as well as an advanced degree in Chemistry will be differentiating factors among candidates. Interested candidates should send a CV and cover letter to ChemCAD 120 Rue du General Gouraud 67210 Obernai France ChemCAD (www.chemcad.fr) breidt uit en zoekt nu mogelijkheden om in Nederland en België scheikundige software te verstrekken. Wij zoeken naar een Nederlands sprekend persoon die met onze afnemers in België en Nederland kan onderhandelen. Om precies te zijn: deze persoon zal verantwoordelijk zijn voor het commerciële succes van onze "Advanced Chemistry Development" software producten in Nederland en Vlaanderen. Ook zal van deze persoon verwacht worden dat hij het trainen van anderen in deze regio coördineert. De geslaagde kandidaat zal een zelf motiverende persoon zijn, die zich gemakkelijk thuis voelt in de gebieden van Verkoop, training en onderhoud van klanten. Omdat onze producten bijzonder gespecialiseerd zijn is het van uiterst belang dat de kandidaat ervaring heeft in NMR, MS of HPLC. (alle drie zou ideaal zijn). Ervaring, succes in de ontwikkeling van nieuwe zaken, het onderhoud van bestaande klanten en een Universitaire opleiding in de scheikunde zullen de belangrijkste criteria zijn bij het kiezen van de kandidaat. Geïnteresseerde kandidaten kunnen hun gegevens sturen naar: ChemCAD 120 Rue du General Gouraud 67210 Obernai France ****************************************************************************** From: jsherratt@vrs-uk.net (John Sherratt) Date: 11 Jan 2002 08:59:42 -0800 Subject: Bioanalytical Mass Spectroscopist Required, UK Base Organization: http://groups.google.com/ Our client is a major development services company providing contract research to the pharmaceutical, chemical and agrochemical industries. The bioanalytical laboratories are equipped with state of the art analytical instrumentation utilising the latest automation and management systems. There is an urgent requirement for a Mass Spectroscopist to develop quantative assays for the analysis of a diverse range of biological fluids. The role will entail the management of client projects during the development phase, a process that will involve close liason with the client. The methods need to be GLP compliant and once implemented you will oversee the operation and/or operate the mass spectrometers. As a technical specialist in your area, you will mentor and train other members of the team and manage the project team. With a degree in a chemistry related subject, you will have at least 3 years bioanalysis experience and have knowledge of developing quantative biological assays. LC-MS or LC-MS-MS experience and knowledge is essential and ideally you will be familiar with HPLC. Good communication skills and previous project management experience would be advantageous. For further details contact John Sherratt on: +44 161 976 4000 or email: jsherratt@vrs-uk.net ****************************************************************************** From: "Fred" Date: Mon, 14 Jan 2002 21:30:42 +0100 Subject: Maximum flow rate with API2 from Finnigan Organization: Guest of ProXad - France What is the maximum flow rate applicable to an API2 ESI interface from Finnigan ? Matt, Iam sure you have the answer ... F. Poignant ****************************************************************************** From: Dan Greenspan Date: Tue, 15 Jan 2002 14:05:31 -0500 Subject: Writing software for Varian Saturn Mass SPec Organization: JHU Applied Physics Laboratory Hello all: I am attempting to write software to manipulate and create Varian Chromatography Workstation Data Files (SMS files). Very little documentation comes with the unit (and it only demonstrates READING the files, not writing) and reading header files isn't sufficient. I have managed to create some malformed files, but can get no further. Does anyone know how I can get, say, an example program showing how to create an SMS file, or some kind of instructions for doing the same? Varian isn't very accessible. Thanks in advance. --------------------------- Dan Greenspan Mail Stop 14-707M Johns Hopkins University Applied Physics Laboratory 11100 Johns Hopkins Road Laurel, MD 20723-6099 240-228-7490 daniel.greenspan@jhuapl.edu --------------------------- ****************************************************************************** From: "Graeme Robertson" Date: Wed, 16 Jan 2002 23:34:18 -0000 Subject: UNKNOWN MASS SPECTRUM (plant derived) Organization: * I would be grateful for any suggestions as to the identity of this volatile plant compound. Mass Spectrum 70eV as follows:- m/z27(8+ACU-),41(6),43(23),52(11),53(13),54(19),71(33),98(100) base peak,99(5), 138(31)molecular ion??. I have searched WILEY and NIST libraries but found no good match. THANKS. ****************************************************************************** From: David Sparkman Date: Fri, 18 Jan 2002 14:07:52 -0500 Subject: RE: UNKNOWN MASS SPECTRUM (plant derived) Organization: * Look at the mass spectrum of 3-(2-Oxo-2,3-dihydrooxazol-5-yl)propionitrile in NIST98 (NIST No. 190196, MW 138, no CAS No., C6H6N2O). Regards; David O. David Sparkman Consultant-At-Large ods@compuserve.com Antioch, CA 94531 1-925-754-5003 M ****************************************************************************** From: David Sparkman Date: Mon, 21 Jan 2002 08:30:24 -0500 Subject: Petroleum mass scale Organization: * Can anyone tell me about a mass scale used by petroleum chemist? I think it is called the Kinderick scale and it is based on CH2 units. Regards; David O. David Sparkman Consultant-At-Large ods@compuserve.com Antioch, CA 94531 1-925-754-5003 ****************************************************************************** From: andy@microlc.com (Andy Moreno) Date: 21 Jan 2002 15:21:55 -0800 Subject: Four (4) Column Production Chemist Positions (FTE) Needed! Organization: http://groups.google.com/ Four (4) Column Production Chemist Positions (FTE) -B.S. Chemistry -Experienced in Capillary Column Production & QA -Willing to relocate to Vista, CA in 2002 (See New MTS Location) -Only qualified candidates will be contacted. Send Resumes to: Micro-Tech Scientific Careers: andy@microlc.com ****************************************************************************** From: Lars Hagen Date: Tue, 22 Jan 2002 12:15:03 +0100 Subject: MALDI Organization: * Hello ! We are looking for a new MALDI-TOF MS to do our analyses of protein, peptides and oligonucleotides. But there are different producers which tell us that their MALDI is the best. So are there anyone who want to share their experience and hopefully give us some objective advice ? Kind regards Lars Hagen ****************************************************************************** From: Larry Phillips Subject: Re: Petroleum mass scale Date: Tue, 22 Jan 2002 07:58:35 -0500 Organization: * David Sparkman wrote: } }Can anyone tell me about a mass scale used by petroleum chemist? I think }it is called the Kinderick scale and it is based on CH2 units. }Regards; }David }O. David Sparkman }Consultant-At-Large }ods@compuserve.com }Antioch, CA 94531 }1-925-754-5003 } Dear David, I believe you are asking about the "Kendrick scale", which appeared in Analytical Chemistry 35 (1963) 2146. Hope this helps. Sincerely, Larry Phillips Developmental Therapeutics Program National Cancer Institute Frederick, MD 21701 USA 301-846-1234 LP5Q@NIH.GOV ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Petroleum mass scale Date: Tue, 22 Jan 2002 15:37:21 GMT Organization: JEOL USA, Inc. David, The Kendrick mass scale assigns the mass of a methylene (CH2) to exactly 14.0000. This allows one to easily view the mass defects of members of a homologous series of alkyl-substituted compounds in a complex mixture. The reference is: E. Kendrick, Analytical Chemistry, 35 (1963), 2146. Chip Cody In David Sparkman writes: }Can anyone tell me about a mass scale used by petroleum chemist? I think it is called the Kinderick }scale and it is based on CH2 units. }Regards; }David }O. David Sparkman }Consultant-At-Large }ods@compuserve.com }Antioch, CA 94531 }1-925-754-5003 -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ==============[ Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: "Frederik" Date: Tue, 22 Jan 2002 16:51:14 +0100 Subject: looking for HP Apollo 400 computer Organization: LOSH Hello, Recently the computer attached to our mass spectrometer broke up (probably a mainboard problem). It is a Hewlett Packard of the Apollo 400 series. Since this is a very old machine, HP has informed us chances are low to find another one. Does anyone know about a computer of this type that is not being used anymore? thanks, Frederik ****************************************************************************** From: "Johnie Brown" Date: Tue, 22 Jan 2002 19:14:19 -0500 Subject: Kinderick Mass Scale Organization: * David I'm sure that you have already got many answers to your question about the Kendrick (sp?) mass scale. You are correct, it is based on a modification of the mass scale to make CH2 weigh exactly 14.0000. Using this mass scale makes the fractional part of the mass equal for all members of a homologous series. This is a very useful way to present accurate mass data for petroleum and its products when performing low-eV or FI/FD analysis using an ultra-high resolution instrument. To make the conversion you either use a Kendrich(sp?) mass reference file or you do the conversion after assigning the normal (carbon-12 =3D 12.0000) accurate masses by multiplying by 0.9988834. = CH2, the repeating unit in each z-series weighs 14.015650 in the = C-12=3D12.0000 scale. For example: formula C-12=3D12.0000 Mass CH2=3D14.0000 Mass C8H14 110.10955 109.9866 C9H16 124.12520 123.9866 C10H18 138.14085 137.9866 C11H20 152.15650 151.9866 This makes it very easy to find your way around in accurate-mass petroleum data and to check it for correct mass assignment. Successively heavier members of each z-series usually go through a smooth distribution of intensities. Any discontinuities in a z-series distribution indicates an area of possible bad mass assignment in the data. Having the mass-intensity data table before your eyes with the accurate masses expressed using the Kendrich scale makes it very easy to find the miss-assigned peak so that you can re-calculate the mass scale for your next iteration. =20 Once you have calibrated the mass scale correctly, you end up with a raw data file consisting of the 1,000 to 3,000 measured mass peaks. The next step is to remove all fragment peaks, if it is Low-eV EI data. As the fragment contributions are removed the fragment isotope contributions to the intensity and fractional mass of adjacent mass peaks must be calculated. Next, to push the method beyond the resolution performance of the instrument, the intensities of z-series that were detected at lower mass can be deconvoluted at higher masses based on the precise mass assignment. Each petroleum group has it's own consistent set of iterative steps to produce a final set of data that can be used to show the intensity distribution of each detected z-series in the sample. This used to be the ultimate table of data to allow the design of the refining process to produce a suite of products and to maximize the profit from a given crude oil. I can't remember the correct spelling. But, if you need, I'm sure I can find it. Johnie }Subject: Petroleum mass scale }Organization: * }Can anyone tell me about a mass scale used by petroleum chemist? I think it is called the Kinderick scale and it is based on CH2 units. }Regards; }David O. David Sparkman }Consultant-At-Large }ods@compuserve.com }Antioch, CA 94531 }1-925-754-5003 ************************************************************************ [ Johnie Brown, Ph.D. Office Phone: (614) 292 - 1271 [ Associate Director, Mass Spectrometry [ OSU CCIC MS Lab Main Lab Phone: (614) 292 - 4821 [ Room 4128 Newman and Wolfrom [ 100 West 18th Avenue Sector Lab Phone: (614) 292 - 0689 [ Columbus, Ohio 43210 [ Fax: (614) 292 - 5955 [ Email: jbrown@ccic.ohio-state.edu [ Web Site: http://www.ccic.ohio-state.edu [ ************************************************************************ ****************************************************************************** From: "Yves KREBS" Date: Wed, 23 Jan 2002 08:22:11 +0100 Subject: Re: looking for HP Apollo 400 computer Organization: * Hello, Yes I use always my Apollo 400, and I have one I my cupboard in reserve. where do you live ? I am in switerland Yves "Frederik" wrote in message news:a2k2nf$pll$1@news-int.gatech.edu... } Hello, } } Recently the computer attached to our mass spectrometer broke up (probably a } mainboard problem). It is a Hewlett Packard of the Apollo 400 series. Since } this is a very old machine, HP has informed us chances are low to find } another one. } Does anyone know about a computer of this type that is not being used } anymore? } } thanks, } } Frederik } } } } ****************************************************************************** From: gazhang@sina.com (gary) Date: 22 Jan 2002 23:34:35 -0800 Subject: Re: MALDI Organization: http://groups.google.com/ Applied Biosystems, Thermo Finnigan and Micromass are all good producers. Lars Hagen wrote in message news:... } Hello ! } } We are looking for a new MALDI-TOF MS to do our analyses of protein, } peptides and oligonucleotides. But there are different producers which } tell us that their MALDI is the best. So are there anyone who want to } share their experience and hopefully give us some objective advice ? } } } Kind regards } } Lars Hagen ****************************************************************************** From: Heino Prinz Date: Wed, 23 Jan 2002 17:48:03 +0100 Subject: Re: MALDI Organization: GWDG, Goettingen Am 22 Jan 2002 23:34:35 -0800 schrieb gazhang@sina.com (gary) }Applied Biosystems, Thermo Finnigan and Micromass are all good producers. } yes, but you should not forget Bruker and their excellent research machine. In general, one should take a sample and do ones own measurements with the MALDI of choice just to see if it can be handled with ease by all kinds of users. Viele Gruesse Heino Prinz Tel.:(++49)-231-133-2480 Fax: (++49)-231-133-1038 ****************************************************************************** From: David Sparkman Date: Wed, 23 Jan 2002 12:23:49 -0500 Subject: Re: MALDI Organization: * I was not aware that Themo-Finnigan had a MALDI. I would look at Bruker, also. Regards; David Regards; David O. David Sparkman Consultant-At-Large ods@compuserve.com Antioch, CA 94531 1-925-754-5003 M ****************************************************************************** From: Werner Spahl Date: Thu, 24 Jan 2002 09:52:15 +0100 Subject: Re: MALDI Organization: [posted via] Leibniz-Rechenzentrum, Muenchen (Germany) On Wed, 23 Jan 2002, David Sparkman wrote: } I was not aware that Themo-Finnigan had a MALDI. I think they don't have a MALDI. But then Shimadzu also has one... -- Werner Spahl (spahl@cup.uni-muenchen.de) Freedom for "The meaning of my life is to make me crazy" Vorlonships ****************************************************************************** From: Laura Date: Wed, 23 Jan 2002 05:16:40 +0000 Subject: Trypsin use in PMF Organization: The University of Liverpool Can anyone tell me why trypsin is preferentially used over other proteases such as Lys C for peptide mass fingerprinting. Thanks, Laura. ****************************************************************************** From: Steve_Missler@accessbusinessgroup.com Date: Thu, 24 Jan 2002 10:49:33 -0500 Subject: LC-MS Organization: * I will be developing LC-MS methods for use in QA laboratories, and am interested in established acceptance criteria for system performance. I know that some organizations such as EPA have set such criteria for GC-MS methods (e.g., DFTPP tuning criteria, initial calibration acceptance criteria, etc. for SW846 method 8270). Has anyone established something along the same lines for LC-MS methods? Thanks ****************************************************************************** From: "Hélène LAVANANT" Date: Thu, 24 Jan 2002 17:31:45 +0100 Subject: isotope simulators Organization: CRIHAN I'm looking for a user-friendly program (preferably freeware) that would simulate isotopic distributions that I could install on a Unix workstation (FreeBSD et HP-UX). This would be for academic purposes. Thanks ! Hélène Lavanant ****************************************************************************** From: jsherratt@vrs-uk.net (John Sherratt) Date: 24 Jan 2002 09:54:37 -0800 Subject: Lab Technician – HPLC/Mass Spectrometry Support, UK Base Organization: http://groups.google.com/ Huge expansion at this highly successful research-driven biopharmaceutical company. They are focused on the discovery, development and commercialisation of innovative products for the treatment of amongst others, cancer and diabetes. The establishment of a new laboratory facility has created an opportunity for a Laboratory technician to support the mass spectrometry and HPLC services to the chemistry department. Under the supervision and expert training of a Senior Mass Spectroscopist, you will assist in the operation and maintenance of MUX-4 analytical LC-MS system, UV and mass directed purification equipment, and HPLC kit. Other responsibilities include instrument calibration, ensuring the smooth running of the service and keeping records of experimental work. This role would be ideal for an A-level or HND/HNC qualified candidate with 6-24 months experience in a laboratory. Experience of HPLC is essential and ideally you will be familiar with mass spectrometry. You will benefit from extensive on the job training and offer a vital service to the department. An ideal opportunity for someone looking for a laboratory technician role and to work with cutting edge technology. For further details contact John Sherratt on: +44 161 976 4000 or email: jsherratt@vrs-uk.net ****************************************************************************** From: Heino Prinz Date: Thu, 24 Jan 2002 19:18:01 +0100 Subject: Re: Trypsin use in PMF Organization: GWDG, Goettingen }Can anyone tell me why trypsin is preferentially used over other }proteases such as Lys C for peptide mass fingerprinting. } 1. It works all right. (most others also do, but this one has been around for a long time) 2. It cleaves at the C-terminal side of basic amino acids. That gives a high abundancy af doulbly charged peptides in ESI-MS, which can easily be employed for MS/MS identification. (The predominant Y-series often allows partial sequencing) Many regards Heino Prinz Tel.:(++49)-231-133-2480 Fax: (++49)-231-133-1038 ****************************************************************************** From: Karla R Date: Fri, 25 Jan 2002 00:00:02 GMT Subject: ICP-MS Question on Potassium Organization: AT&T Worldnet Hi All! I'm trying to analyze for potassium (matrix includes vanadium at 99.9%, HNO3 & HF) on the ICP-MS. I'm analyzing in at the ppb level. I can't seem to get a half decent calibration curve. I know there is an ArH overlap at the mass I'm using, but it doesn't seem to be interfering. I recently took a class for my instrument, and we did analyze for potassium at some point (also in ppb range). But I can't seem to do it. Anybody have advice on this. Or any links I could take a look at. It would be appreciated. Feel free to respond to me by e-mail or by the news group. Thanks in advance. Karla ****************************************************************************** From: erik@futurebiojobs.com (Erik Sayle) Date: 24 Jan 2002 23:40:52 -0800 Subject: Biotech Jobs of tomorrow at www.FutureBioJobs.com Organization: http://groups.google.com/ Biotech Jobs of tomorrow at www.FutureBioJobs.com We list biotech jobs cutting edge areas. If you are looking for work or not, you can register for our email job alerts which are delivered to your email box. We will send you jobs & more that relate to your interests and skills. http://www.futurebiojobs.com Search and Apply for Jobs in: Genomics, Molecular Biology, Microarrays, & DNA Chips Bioinformatics, Computational Biology & Cheminformatics Bioengineering, Microfluidics, BioMEMS & Medical Devices Proteomics & Protein Chips Chemistry, Medicinal Chemistry Drug Discovery, Pharmaeuticals ********************************************** Aclara Sr.Scientist-3 - Protein chemistry http://www.futurebiojobs.com/jobview.cfm?jid=71 Mountain View CA USA Posted @ 01/02/02 Sr. Mechanical Engineer http://www.futurebiojobs.com/jobview.cfm?jid=69 Mountain View ca USA Posted @ 01/02/02 Sr. Optics Engineer http://www.futurebiojobs.com/jobview.cfm?jid=70 Mountain View ca USA Posted @ 01/02/02 Sr. Automation Engineer http://www.futurebiojobs.com/jobview.cfm?jid=68 Mountain View CA USA Posted @ 01/02/02 Research associate-3/4 http://www.futurebiojobs.com/jobview.cfm?jid=67 Mountain View CA USA Posted @ 01/02/02 Research Associate/Sr. RA, Assay Development http://www.futurebiojobs.com/jobview.cfm?jid=66 Mountain View CA USA Posted @ 01/02/02 Research Associate, PCR Applications http://www.futurebiojobs.com/jobview.cfm?jid=65 Mountain View CA USA Posted @ 01/02/02 Research Associate II, PCR Applications http://www.futurebiojobs.com/jobview.cfm?jid=63 Mountain View CA USA Posted @ 01/02/02 Document Control Specialist (6 month Contract) http://www.futurebiojobs.com/jobview.cfm?jid=61 Mountain View CA USA Posted @ 01/02/02 Quality Assurance Temp $14- $16 per hour http://www.futurebiojobs.com/jobview.cfm?jid=62 Mountain View CA USA Posted @ 01/02/02 Research Associate III/IV http://www.futurebiojobs.com/jobview.cfm?jid=64 Mountain View CA USA Posted @ 01/02/02 Director of Marketing http://www.futurebiojobs.com/jobview.cfm?jid=60 Mountain View CA USA Posted @ 01/02/02 Assoc Dir, Manufacturing Engineering http://www.futurebiojobs.com/jobview.cfm?jid=42 Mountain View CA USA Posted @ 12/21/01 Chemist - Sr. Chemist http://www.futurebiojobs.com/jobview.cfm?jid=44 Mountain View CA USA Posted @ 12/21/01 Axiom Resources Web Developer http://www.futurebiojobs.com/jobview.cfm?jid=140 San Mateo CA USA Posted @ 01/10/02 Entigen Bioinformatics Software Developer http://www.futurebiojobs.com/jobview.cfm?jid=41 Sunnyvale CA USA Posted @ 12/20/01 Keck Graduate Institute of Applied Life Faculty Opening - Computational Biology http://www.futurebiojobs.com/jobview.cfm?jid=152 Claremont CA USA Posted @ 01/22/02 Faculty Opening - Bioengineering http://www.futurebiojobs.com/jobview.cfm?jid=151 Claremont CA USA Posted @ 01/22/02 Faculty Openings - Systems Biology & Signal Transduction http://www.futurebiojobs.com/jobview.cfm?jid=150 Claremont CA USA Posted @ 01/22/02 Res Associate: Gene Expression Profiling Core http://www.futurebiojobs.com/jobview.cfm?jid=154 Claremont CA USA Posted @ 01/22/02 Post-Doctoral Positions (7 different positions) http://www.futurebiojobs.com/jobview.cfm?jid=153 Claremont CA USA Posted @ 01/22/02 Lumicyte Vice President of Proteomics and Discovery http://www.futurebiojobs.com/jobview.cfm?jid=72 Fremont CA USA Posted @ 01/02/02 Senior Scientist, Ion Physicist http://www.futurebiojobs.com/jobview.cfm?jid=54 Fremont CA USA Posted @ 12/31/01 Senior Patent Specialist Biotech http://www.futurebiojobs.com/jobview.cfm?jid=53 Fremont CA USA Posted @ 12/31/01 Research Associate, Analysis (multiple positions) http://www.futurebiojobs.com/jobview.cfm?jid=52 Fremontq CA USA Posted @ 12/31/01 Project Team Leader, Biomarker Discovery (multiple positions) http://www.futurebiojobs.com/jobview.cfm?jid=45 Frement CA USA Posted @ 12/27/01 Lynx Research Associate http://www.futurebiojobs.com/jobview.cfm?jid=148 Hayward CA USA Posted @ 01/22/02 Senior Mechanical Engineer http://www.futurebiojobs.com/jobview.cfm?jid=147 Hayward CA USA Posted @ 01/22/02 Director, Discovery Collaborations http://www.futurebiojobs.com/jobview.cfm?jid=146 Hayward CA USA Posted @ 01/22/02 Researh Associate http://www.futurebiojobs.com/jobview.cfm?jid=149 Hayward CA USA Posted @ 01/22/02 Molecular Reflections Surface Chemist - (level depends on experience) http://www.futurebiojobs.com/jobview.cfm?jid=56 San Diego CA USA Posted @ 12/31/01 Protein Biochemist with background in GPCR and Kinase Assays http://www.futurebiojobs.com/jobview.cfm?jid=55 San Diego CA USA Posted @ 12/31/01 Sanford Rose Assoc, San Francisco VP/Sr. Dir. 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Director/Director, Statistics http://www.futurebiojobs.com/jobview.cfm?jid=74 Confidential USA Posted @ 01/02/02 Associate Director, Bioinformatics http://www.futurebiojobs.com/jobview.cfm?jid=73 Confidential USA Posted @ 01/02/02 Array Processing Project Leader http://www.futurebiojobs.com/jobview.cfm?jid=58 USA Posted @ 12/31/01 UC Riverside Postdoc, Microfluidics http://www.futurebiojobs.com/jobview.cfm?jid=57 Riverside CA USA Posted @ 12/31/01 UC Santa Cruz Faculty Positions in Bioinformatics & Biomolecular Engineering http://www.futurebiojobs.com/jobview.cfm?jid=155 Santa Cruz CA USA Posted @ 01/23/02 ****************************************************************************** From: Tony Hassett Date: Fri, 25 Jan 2002 11:48:04 +0200 Subject: MS in South Africa Organization: University of Pretoria Hi, Anyone interested in Mass Spectrometry in South Africa (conferences, jobs, etc) visit the South African Association for Mass Spectrometry web site at: http://ww.up.ac.za/academic/acadorgs/saams/ Regards Tony ****************************************************************************** From: jprudhomme@ibcusa.com (James Prudhomme) Date: 25 Jan 2002 06:23:07 -0800 Subject: IBC's Proteomics Conference Organization: http://groups.google.com/ *** CONFERENCE ANNOUNCEMENT *** PROTEOMICS 2002 MAY 6-9, 2002 PHILADELPHIA, PA www.LifeSciencesInfo.com/proteomics IBC's Annual Proteomics 2002 is the premier event designed to address the critical elements that are driving this field towards drugable targets. In particular, this meeting will address: * How will sample complexity reduction be tackled? * Will gel and LC based approaches merge to map out complex Proteomes? * How will the latest developments in MS hardware influence protein characterization? * How will we tie all this information together and still meet the objectives of understanding the underlying Biology? FREE EXHIBIT HALL ONLY PASSES ARE AVAILABLE UNTIL APRIL 22, 2002! The FREE Exhibit Hall Pass will entitle you to: * Hands-on Exposure to the Latest Technologies * New Product Launches * Poster Presentations * Networking Cocktail Receptions Get your FREE Exhibit Hall Pass On-line: www.LifeSciencesInfo.com/proteomics (After April 22, 2002 the exhibit hall pass is $25.) TEAM DISCOUNTS ARE AVAILABLE - FOURTH GOES FREE: IBC welcomes your team members to facilitate interdisciplinary networking and learning. When three members of the same company register for the conference at the same time, the fourth attends for FREE! Complete registration forms must be sent together with complete payments for the entire group to qualify for team discounts. No partial payments or registrations sent without payment are eligible for this discount. Please contact me if you want a detailed program agenda for this event or if you have any questions via email: jprudhomme@ibcusa.com HOW TO REGISTER: Phone (508) 616-5550 x1004 Fax (508) 616-5522 E-mail reg@ibcusa.com Online www.LifeSciencesInfo.com Mail IBC USA Conferences, Inc. One Research Drive, Suite 400A Westborough, MA 01581-5195 - Jim Prudhomme Life Sciences Marketing IBC USA Conferences, Inc. ****************************************************************************** From: John Hill Date: Fri, 25 Jan 2002 16:10:10 +0000 Subject: VG ZAB-2SE Organization: * Hello All We have a VG ZAB-2SE sector mas spectrometer that we would like to dispose of either as a complete instrument or as spares. Space is required to make way for building work and eventually the installation of a new high resolution instrument. If anybody is interested contact me, the instrument is located in central London. Many thanks John Hill Mass Spectrometry Facility University College London E-mail: j.t.hill@ucl.ac.uk ****************************************************************************** From: Christian Lindh Date: Fri, 25 Jan 2002 17:37:49 +0100 Subject: Possible postdoc position. Organization: * The Department of Occupational and Environmental Medicine at the University of Lund Sweden has received a grant from the Swedish council for working life and social sciences for the project “Biological monitoring of carcinogenic compounds in the rubber industry by analysis of protein adducts”. The project will mainly focus on the development of very sensitive GC-MS and LC-MSMS methods for detecting adducts of butadiene and benso[a]pyrene metabolites on albumin and hemoglobin. The methods will then be applied in exposed workers in the rubber industry. We seek a postdoc experienced in development of methods for GC-MS and LC-MSMS, the project will also involve synthesis work so experience in organic chemistry is an advantage. The Department in Lund is well equipped and has an excellent mass spectrometer facility. Please send a notification of interest including a CV and the addresses of possible references to: christian.lindh@ymed.lu.se For further information please contact Dr. Christian Lindh, Department of Occupational and Environmental Medicine, University Hospital, SE-22185 Lund, Sweden. ****************************************************************************** From: Joerg Hau Date: Fri, 25 Jan 2002 20:21:43 +0100 Subject: Re: isotope simulators Organization: I speak for myself Bonjour Hélène, } I'm looking for a user-friendly program (preferably freeware) that } would simulate isotopic distributions that I could install on a Unix } workstation (FreeBSD et HP-UX). If you have Internet access, there is an isotope calculator online at http://www.chemcalc.org ... another one is at http://medlib.med.utah.edu/masspec/ As for stand-alone applications, a search for "isotop" on freshmeat.net turned up one (!) program, which is provided under GPL. If you can live with a command-line version, I have also put such a program on my website (see link below; in the Mass Spectrometry section). This program is published under the GNU Public License, so the source code is - of course - provided. Let us know if this helps ... Greetings from Lausanne! - Joerg -- joerg.hau(at)swissonline.ch * Lausanne, Switzerland http://www.mysunrise.ch/users/joerg.hau/ "All standard disclaimers apply". ****************************************************************************** From: gergana.genova@metalife.de (Gergana Genova) Date: 26 Jan 2002 09:28:34 -0800 Subject: New BIOINFORMATICS Resource Launched! Organization: http://groups.google.com/ Dear Coleagues, In the beginning of the year 2002 a team of biologists and programmers launched new FREE bioinformatics resource. This site offers: - collected information in searchable databases [incl. GBK, SPRT, PIR and many of major databases available]; - Algorithms [Blast, ClustlW, 3D modeler, 2D Prediction and many others] - User can save their files generated by algorithms and search processes. Servers are placed in Bulgaria, at the following address: http://www.metalife.de Feel free to contact METALIFE team. Best Regards Gergana Genova METALIFE ****************************************************************************** From: kmurray@ch335c.chem.lsu.edu (K. Murray) Date: 26 Jan 2002 22:32:33 GMT Subject: Re: isotope simulators Organization: Louisiana State University In article Joerg Hau writes: } Bonjour Hélène, } } } I'm looking for a user-friendly program (preferably freeware) that } } would simulate isotopic distributions that I could install on a Unix } } workstation (FreeBSD et HP-UX). } } If you have Internet access, there is an isotope calculator online at } http://www.chemcalc.org ... another one is at } http://medlib.med.utah.edu/masspec/ } } As for stand-alone applications, a search for "isotop" on } freshmeat.net turned up one (!) program, which is provided under GPL. } } If you can live with a command-line version, I have also put such a } program on my website (see link below; in the Mass Spectrometry } section). This program is published under the GNU Public License, so } the source code is - of course - provided. } } Let us know if this helps ... } } Greetings from Lausanne! } } - Joerg } } } -- } joerg.hau(at)swissonline.ch * Lausanne, Switzerland } http://www.mysunrise.ch/users/joerg.hau/ } "All standard disclaimers apply". I haven't seen a Unix isotope pattern calculator, but there are a few others on the Web in addition to the two mentioned above: Molmass (Java with downloadable 'lite' version) http://www.oraxcel.com/projects/molmass/index.htm ChemCalc http://www.geocities.com/junhuayan/pattern.htm SIS MS Tools http://www.sisweb.com/cgi-bin/mass10.pl Sheffield Chemputer http://www.shef.ac.uk/~chem/chemputer/isotopes.html For stand-alone there is Isopro for Windows http://members.aol.com/msmssoft/ and the Macintosh Isotope Pattern Calculator http://info-mac.acornsw.com/infomac/_SCIENCE_AND_MATH/ISOTOPE-PATTERN-CA LC-166.HTML I have most of these in the software directory at Basepeak http://www.spectroscopynow.com/Spy/basehtml/SpyH/1,1153,4-4-791-0-791-di rectories-0-0,00.html or if that's horribly mangled in the line wrap, look under the "Directory>Software>Calculators" and "Directory>Software>WWW Based Calculations" headings at http://www.spectroscopynow.com/Spy/ms/ If you find something that you want listed, send me an e-mail or us the site input form. -- Kermit Murray http://ch335c.chem.lsu.edu/ ****************************************************************************** From: Paul Gooden Date: Sun, 27 Jan 2002 06:32:45 -0800 (PST) Subject: Re: MALDI Organization: * Amersham (formerly Pharmacia) is also in the MALDI-TOF market now. Lars Hagen wrote: }------------------------------------------------- }Hello ! } }We are looking for a new MALDI-TOF MS to do our analyses of protein, }peptides and oligonucleotides. But there are different producers which tell us that }their MALDI is the best. So are there anyone who want to }share their experience and hopefully give us some objective advice ? } } }Kind regards } }Lars Hagen } } }__________________________________________________ }Do You Yahoo!? }Great stuff seeking new owners in Yahoo! Auctions! }http://auctions.yahoo.com ****************************************************************************** From: "Simon" Date: Mon, 28 Jan 2002 14:59:36 +0100 Subject: HELP: protein proton affinity Organization: Universita' degli Studi di Milano Good day, I am an Italian chemical researcher and I study proteins and peptides by mass spectrometry. I would like to know if exist a software tool, both on line or to download, in order to calculate the theoretical proton affinity of a protein if a known sequence is given. If you can halp me please send me an E-MAIL at the follow address: dtoycr@tin.it Thank you very much for your help Simone ****************************************************************************** From: "Daria Jouravleva" Date: Tue, 29 Jan 2002 11:48:00 -0500 Subject: Do you check pKa and solubility before the experiment? Organization: * I've heard that some use pKa prediction (in our case, ACD/pKa DB) to evaluate ionizable forms of a novel compound and to develop appropriate LCMS conditions (if the compound does indeed ionize). More importantly, many samples which show good solubility in DMSO or another organic solvent do not have sufficient solubility in a water-based buffer. Given the fact that many LC gradients start with a high water content in a buffer, precipitation inside LCMS apparatus has been known to occur, clogging the column or bleeding through the whole set. It appears tempting to use solubility prediction tools to deal with that issue. Does anyone do that? What other things one should check for while dealing with a new or a vaguely known sample? I'd appreciate your expert advice. Daria Jouravleva, Ph.D. ____________________________________________________________________________ _ Join us at the North American User’s Meeting: Mar 7-8, Princeton NJ Guest Speakers: Gary Martin, Pharmacia and Chris Lipinski from Pfizer. ____________________________________________________________________________ _ Advanced Chemistry Development, Inc. 90 Adelaide St.West, Suite 702 Toronto, Ontario M5H 3V9 CANADA Phone: (416) 368-3435 ext 248 US & Canada: (800) 304-3988 Fax: (416) 368-5596 E-mail: daria@acdlabs.com URL: http://www.acdlabs.com/ ****************************************************************************** From: Peter.Harrington@Ohio.edu (Peter de B. Harrington) Date: 29 Jan 2002 09:59:06 -0800 Subject: APCI-ToF-MS Organization: http://groups.google.com/ I'm interested in buying an API-ToF-MS for a high throughput screening project of natural products. Any recommendations on brands. Photoionization sources look like they may be advantageous to the project. Please e-mail me a copy of any reply posts. TIA, Pete ****************************************************************************** From: "Jeff Whitney" Date: Tue, 29 Jan 2002 15:47:46 -0500 Subject: Re: Qualitative confirmation on Xcalibur Organization: Verio We have significant experience in developing custom processing applications that integrate with the Xcalibur system. Give me a call if you are interested in this type of consulting/custom software development. Jeff Whitney Novatia, LLC 301A College Road East Princeton, NJ 08540 609-951-0181, x1 whitneyj@enovatia.com www.enovatia.com "gerard" wrote in message news:9v55ac$6uu$1@news-int.gatech.edu... } I use a GCQ to screen 30 pesticides in fresh fruits and vegetables. } } To confirm the positives, I have been going to the Qual Browser of } Xcalibur and manually check the ion ratios of the positives and } compare them with the ones in the standards. This is repetitive. I } was wondering if anybody has been able to automate that step and } produce a report. } } I tried to include the ion ratio confirmation table in one of my } custom report but without much success. For some reason, the ions and } their ratios get switched around. } } I also would like to know if anybody is confirming their positives in } some other way. } } If anyone has any advice it would be greatly appreciated, } Thank you, } } G Gerard } } ****************************************************************************** From: "Jeff Whitney" Date: Tue, 29 Jan 2002 16:05:53 -0500 Subject: MassLynx External Programming Interface Organization: Verio Does anyone know where I can get details on how to externally program to the MassLynx software. Specifically, I want to automatically fire an external executable (a custom program) from within MassLynx, either on-the-fly during a sample analysis or via post acquisition batch processing. I also need to pass run specific information from the sample/sequence into the custom program and get at the spectra in the datafiles. Does MassLynx/MicroMass provide such a programming interface? Any information would be much appreciated. Jeff Whitney ****************************************************************************** From: "Dave White" Date: Tue, 29 Jan 2002 18:01:29 -0600 Subject: Re: MassLynx External Programming Interface Organization: * There are such interfaces in MassLynx, but depending on exactly what you are trying to do, they can get a little cluncky to use. I'm not in the lab now, so can't pull up a copy of MassLynx to see exactly how to do this, but if I recall correctly, ther are columns in the sample list editor which allow you to specify pre and post run executables/batch files which can be run externally. This should take care of your first request. As far as passing info out of MassLynx - there are some 'global status variables' which are continuously written to an ini file, which you can then access from the external application. However, the information passed out is fairly limited - IIRC, the info is written to a file called status.cfg (I may be wrong on that), and you need to tell MassLynx to update the file from a setting in the options page. Then for spectral data, there are two ways to get at this. One is to create a macro to write out a spectral listing to a text file which your application can then read and parse back into usable data. This is slow, and cluncky. The second is to read the raw data file directly, but for this you need the MassLynx file format - I spent many days with a hex editor, a selection of raw data files and stacks of printouts to finally find enough 'patterns' in the data to be able to reverse engineer a number of the formats (and there are many - something like 15 or 20 all together). There is a MassLynx manual (fairly small, meybe 20 or so pages) called Interfacing (or something similar) which describes the file formats for non raw data and the status config file. There is also a help file in a MassLynx directory which explains the Visual Basic macro language interface to MassLynx - although this is a pretty rough implementation - many of the included VB include files don't work with VB5 or 6. Good luck -- Dave White SpectraChrom Software www.spectrachrom.com dave_white@spectrachrom.com "Jeff Whitney" wrote in message news:a3732u$h9d$1@news-int.gatech.edu... } Does anyone know where I can get details on how to externally program to the } MassLynx software. Specifically, I want to automatically fire an external } executable (a custom program) from within MassLynx, either on-the-fly during } a sample analysis or via post acquisition batch processing. I also need to } pass run specific information from the sample/sequence into the custom } program and get at the spectra in the datafiles. Does MassLynx/MicroMass } provide such a programming interface? } } Any information would be much appreciated. } } Jeff Whitney } } } ****************************************************************************** From: "Peter Robinson" Date: Wed, 30 Jan 2002 11:35:49 +1300 Subject: TEFs and detection limits Organization: * Kia Ora all Can anyone tell me if there is a standard way of dealing with Toxic Equivalence Factors (TEFs) where a compound is below the lab detection limit. I guess this also crops up where one is plotting long term trends or trying to sum or average groups of data. We have a specific case where a client has some values for PAHs, but some of the data are 'less than DL'. I have a file a couple of inches thick where I have collected papers on this topic over the years and solutions range from 'ignore anything < DL, treat as zero' through 'use half the DL value' to complicated formulae. I suspect there is no easy answer, but maybe there is a standard which has been adopted for consistency purposes. Regards Peter Robinson, Hill Labs, Hamilton, NZ ###################################################################### Attention: The information in this email and in any attachments is confidential. If you are not the intended recipient then please do not distribute, copy or use this information. Please notify us immediately by return email and then delete the message from your computer. Any views or opinions presented are solely those of the author. ###################################################################### ****************************************************************************** From: G Koster Date: Wed, 30 Jan 2002 09:36:37 +0100 Subject: re: APCI-TOF-MS Organization: * Dear Peter On the subject of photo ionization I can tell you that there are to producers of lc/ms couplings using the photo ionization technique. agilent and Sciex. Sciex has just taken it into production and it is a rebuild CI housing for the 3000 and compatible machines. To do photo ionization you need to get rid of all solvent that have proton interaction else you get protonated species and not radical cations. It can be needed to use a dopand as toluene. Be sure to use the purest. About the lamps that are used are small 10 EV lamps also used in gaschromatochrafie. If you have more questions on photo ionisation please ask me. Grielof koster ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: isotope simulators Date: Wed, 30 Jan 2002 17:28:33 GMT Organization: JEOL USA, Inc. My Mass Spec Tools(TM) software suite (www.chemsw.com) includes an isotope ratio calculator with a large number of features: -- Peak simulation including overlapping compositions and variable resolving power or resolution (quadrupole mode). -- Graphical and text output -- Parsing of nested parentheses in the formula -- Support for user-defined isotopes and isotope labeling calculations -- Very simple user interface -- Very fast calculations, even for larger molecular weights -- Other programs included are elemental composition from exact masses, mass spec periodic table, series calculator, ESI calculator. I have recently added command-line support for automation and web-based calculations or calls from other programs. This will probably be available in a month or so. Chip Cody In kmurray@ch335c.chem.lsu.edu (K. Murray) writes: }In article }Joerg Hau writes: }} Bonjour Hélène, }} }} } I'm looking for a user-friendly program (preferably freeware) that }} } would simulate isotopic distributions that I could install on a Unix }} } workstation (FreeBSD et HP-UX). }} }} If you have Internet access, there is an isotope calculator online at }} http://www.chemcalc.org ... another one is at }} http://medlib.med.utah.edu/masspec/ }} }} As for stand-alone applications, a search for "isotop" on }} freshmeat.net turned up one (!) program, which is provided under GPL. }} }} If you can live with a command-line version, I have also put such a }} program on my website (see link below; in the Mass Spectrometry }} section). This program is published under the GNU Public License, so }} the source code is - of course - provided. }} }} Let us know if this helps ... }} }} Greetings from Lausanne! }} }} - Joerg }} }} }} -- }} joerg.hau(at)swissonline.ch * Lausanne, Switzerland }} http://www.mysunrise.ch/users/joerg.hau/ }} "All standard disclaimers apply". }I haven't seen a Unix isotope pattern calculator, but there are a few }others on the Web in addition to the two mentioned above: }Molmass (Java with downloadable 'lite' version) }http://www.oraxcel.com/projects/molmass/index.htm }ChemCalc }http://www.geocities.com/junhuayan/pattern.htm }SIS MS Tools }http://www.sisweb.com/cgi-bin/mass10.pl }Sheffield Chemputer }http://www.shef.ac.uk/~chem/chemputer/isotopes.html }For stand-alone there is Isopro for Windows }http://members.aol.com/msmssoft/ and the Macintosh Isotope Pattern }Calculator }http://info-mac.acornsw.com/infomac/_SCIENCE_AND_MATH/ISOTOPE-PATTERN-CA }LC-166.HTML }I have most of these in the software directory at Basepeak }http://www.spectroscopynow.com/Spy/basehtml/SpyH/1,1153,4-4-791-0-791-di }rectories-0-0,00.html }or if that's horribly mangled in the line wrap, look under the }"Directory>Software>Calculators" and "Directory>Software>WWW Based }Calculations" headings at }http://www.spectroscopynow.com/Spy/ms/ }If you find something that you want listed, send me an e-mail or us the }site input form. }-- }Kermit Murray }http://ch335c.chem.lsu.edu/ -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ==============[ Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: John Seney Date: Thu, 31 Jan 2002 10:39:29 GMT Subject: Pointer to Oscilloscope FAQ Organization: ATT Broadband http://www.qsl.net/wd1v/scopefaq/index1.html ****************************************************************************** From: bevans@syagen.com (Brent Evans) Date: 1 Feb 2002 12:22:58 -0800 Subject: Re: APCI-TOF-MS Organization: http://groups.google.com/ Several points of clarification are in order. The Agilent APPI source is available for the 1100 Series LC/MSD and Trap systems. This source was developed for Agilent by Syagen Technology, Inc. under the brand name PhotoMate(TM). Agilent has been shipping the product for almost a year now with good results. PhotoMate sources designed for other major MS vendors is forthcoming from Syagen. With respect to TOF MS, Syagen markets an instrument named Radiance Pro(TM). This analyzer uses low pressure photoionization as the source and is fully integrated into a quadrupole ion trap, TOF. The system bypasses the LC step by using direct syringe injection from an intergal autosampler. Additonal information is available at www.syagen.com. Regards, Brent Evans ****************************************************************************** From: ed vd wal Date: Sat, 02 Feb 2002 11:33:25 +0100 Subject: screening with TOF-MS quantitative? Organization: XO Communications B.V. I have a relative simple question: just like Pete (APCI-TOF-MS) i am planning to set up a screening method. I think about using a TOF for obvious reasons. However, can someone tell me if the TOF's which are available now are quantitative? Or what should i do to make it so? This is very importend for the method. Ed ****************************************************************************** From: sms-gmbh@t-online.de (Volker Grau, Ph.D.) Date: 2 Feb 2002 10:33:34 -0800 Subject: Re: VG ZAB-2SE Organization: http://groups.google.com/ Hello John, I´m interested in your data system if you have updated to OPUS. Many thanks Volker John Hill wrote in message news:... } Hello All } } We have a VG ZAB-2SE sector mas spectrometer that we would like to dispose } of either as a complete instrument or as spares. Space is required to make } way for building work and eventually the installation of a new high } resolution instrument. } If anybody is interested contact me, the instrument is located in central } London. } } Many thanks } } John Hill } Mass Spectrometry Facility } University College London } E-mail: j.t.hill@ucl.ac.uk ****************************************************************************** From: hailm@enovatia.com (Mark Hail) Date: 2 Feb 2002 11:32:48 -0800 Subject: Re: screening with TOF-MS quantitative? Organization: http://groups.google.com/ ed vd wal wrote in message news:... } I have a relative simple question: } just like Pete (APCI-TOF-MS) i am planning to set up a screening method. } I think about using a TOF for obvious reasons. However, can someone tell } me if the TOF's which are available now are quantitative? Or } what should i do to make it so? This is very importend for the method. } } Ed Ed, As with any MS-based method, you will probably need internal standards to get good quantitative results. I would consider using an evaporative light scattering detector (ELSD) in-line with your LC/MS system. ELSD gives a response which is proportional to the mass of analyte eluting. The only requirement is that your compound should not be volatile. We are using one from Richard Scientific (http://www.richardscientific.com/products/sedex.html) which works quite well for this purpose. We are also using ESI positive and negative ion switching in a single run, which gives us excellent confirmatory data. For example, often we get both [M+H]+ and [M-H]- ions. We are using an LCQ ion trap and a Michrom MAGIC HPLC (http://www.michrom.com). The MAGIC is great because the gradient delay volume is very low, which is important if you want high throughput. Please feel free to contact us if you need additional details. We are now offering this analysis as a service, if anyone else is interested. Regards, Mark Hail **************************** Novatia LLC 301A College Road East Princeton, NJ 08540 tel: (609) 951-0181 fax: (609) 951-0185 email: hailm@enovatia.com web: http://www.enovatia.com **************************** ****************************************************************************** From: calvert@eos.ncsu.edu (Phil Calvert) Date: Mon, 04 Feb 2002 02:22:41 -0500 Subject: HP GCD or 5971 Organization: North Carolina State University Hi, Anyone else out there using an Hewlett-Packard GCD or 5971? I think we may be having some performance problems (low sensitivity), based on what I am seeing on the tune report, and thought comparing notes with someone else might be useful. Regards, -- Phil Calvert calvert AT eos.ncsu.edu ****************************************************************************** From: Peter Hains Date: 5 Feb 2002 06:05:33 GMT Subject: ANNOUNCEMENT: Mass Spectrometry and 2D Electrophoresis Courses Organization: Australian Proteome Analysis Facility Hello all, The Australian Proteome Analysis Facility (APAF) would like to announce the next in it's continuing courses on 2D Electrophoresis and Mass Spectrometry. For information about dates, cost and content, please follow this link (http://www.proteome.org.au/services/courses.html) and download the Adobe PDF for the MS course or the RTF for the 2D course. Enquiries should be made by e-mail to apafinfo@proteome.org.au. Thank you for your time. Peter Hains -- I'm afraid I don't have a clever saying to put here. Remove NOSPAM to e-mail me. Peter Hains (PhD) Ph. +61 2 9850 6216 Australian Proteome Analysis Facility Fax. +61 2 9850 6200 Level 4 Building F7B Macquarie University, Sydney 2109 ****************************************************************************** From: "Francesc Carrera" Date: Tue, 5 Feb 2002 11:20:34 +0100 Subject: water MS quality Organization: * Hello MS people! Some years ago we developed a LC-UV-MS generic method for drug discovery new products. After doing some improvements in this method (regarding column, flows, mobile phase composition, temperature,...) now we would like to reduce the noise of MS chromatogram and we have tried to change the quality of the water. We have obtained different noise by using Milli-Q quality or HPLC quality bottled water . Milli-Q water produces a good baseline until the percentage of organic phase is increased. Then the noise goes up (mainly m/z=3D282). With HPLC quality bottled water we obtain less ion 282 at the end but more noise at the beginning and when the gradient returns to 100% aquose phase (m/z=3D208). UV noise is similar for both. Have you got any different experiences by using water in LC-MS gradient methods? Do you know if there is some quality water suitable for LC-UV-MS gradients? Francesc Carrera i Carrera Mass Spectrometry System Manager Analysis R&D Department Research Center ALMIRALL PRODESFARMA S.A. Cardener 68-74 08024-Barcelona CATALONIA e-mail: fcarrera@almirallprodesfarma.com "Freedom consists in being able to choose the chains that tie you" ****************************************************************************** From: webmaster@icdd.com (ICDD Webmaster) Date: 5 Feb 2002 06:35:27 -0800 Subject: Spring XRF and XRD Clinics at the International Centre for Diffraction Data (ICDD) Organization: http://groups.google.com/ Spring XRF and XRD Clinics at the International Centre for Diffraction Data (ICDD) Take your XRF & XRD skills to the next level with the ICDD X-ray Clinics! Practical X-ray Fluorescence Spectrometry April 29-May 3, 2002 Fundamentals of X-ray Powder Diffraction June 3-7, 2002 Advanced Methods in X-ray Powder Diffraction June 10-14, 2002 Sharpen XRF & XRD skills with tricks of the trade in specimen preparation, computerized search match, analysis of complex materials and much more from experts of the field. Register today online at www.icdd.com or e-mail clinics@icdd.com for more information. ****************************************************************************** From: jsherratt@vrs-uk.net (John Sherratt) Date: 5 Feb 2002 07:14:47 -0800 Subject: Bioanalytical Study Director, UK Base Organization: http://groups.google.com/ Successful and well-established Contract Analytical Testing consultancy is expanding its capabilities into mass spectrometry. Due to demand from existing and new customers they are to provide a bioanalysis and proteomics service and are looking for an experienced Study Director to head up this new division and guide it through an aggressive growth plan. With a background in bioanalysis and experience of LC-MS-MS method development and validation you will take full responsibility for the operation and ongoing expansion of the new service. This will involve managing a team of bioanalysts, and eventually other more junior Study Directors, and ensuring delivery of projects on time and to budget. You will be the main point of contact for the sponsors so will need good communication skills and commercial awareness. Knowledge of GLP requirements and protocol preparation is essential. This is an exciting opportunity to establish and manage an exciting new department. It would be ideal for experienced Study Directors or Mass Spectroscopists who are looking for their next challenge. For further details contact John Sherratt on: +44 161 976 4000 or email ms@vrs-uk.net ****************************************************************************** From: "chris.lee" Date: Tue, 5 Feb 2002 21:49:17 +0100 Subject: Re: screening with TOF-MS quantitative? Organization: Infonie With a TOF, the microchannel plate detector has a dead-time after the arrival of each ion. As a result, the detector response (current per ion) falls off at high ion counts, possibly at ion currents low enough for ion statistics to still be a significant source of error. Having said that, TOFs are OK for quantitative work if the software lets you know when the ion current is within the acceptable range, and you're into trace rather than high precision analyses. This fundamental limitation of TOFs might come as a bit of a surpise if you're used to other types of instrument, where linear dynamic range is less of a problem - provided coeluting internal standards are used. Regards "ed vd wal" a écrit dans le message news: a3gsvh$3jj$1@news-int.gatech.edu... } I have a relative simple question: } just like Pete (APCI-TOF-MS) i am planning to set up a screening method. } I think about using a TOF for obvious reasons. However, can someone tell } me if the TOF's which are available now are quantitative? Or } what should i do to make it so? This is very importend for the method. } } Ed } } } ****************************************************************************** From: Ken Matuszak Date: Wed, 06 Feb 2002 06:07:34 -0600 Subject: Re: screening with TOF-MS quantitative? Organization: Abbott Labs Pharmaceutical Products Division Ed: I use a Qstar Pulsar for qualititative work to support discovery based ADME (currently permeability screens). There is no question that it's hard to beat a triple for formal quantitation with their great dynamic range. However, we have foound the Pulsar to be an excellent tool in our application, because while the linear dynamic range is less than that of a triple, we are able to make use of the high res of the Pulsar to operate in full scan MS mode instead of MS/MS mode. For us, this is a key advantage, because we are pushing 28-42 new compounds (which generates 4 to 6 - 96 well plates by the time we analyze replicates of the basals, apicals, blanks, and T0s, and their dilution curves) through one instrument with one operator each week. So, there is no time for development. With this technique I have one generic MS setup (full scan 150 to 1000) which is used for almost everything. The Pulsar's high resolving power (10,000 at 1200), allows me to set pretty narrow mass ranges for quantitation (0.1 amu or less), which effectively reduces the chemical noise, in a way similiar to (maybe not as good as) a triple. With the res of the Pulsar, the ion peak is well within the 0.1 amu peak width. The Pulsar also has excellent mass stability (often I have 5 ppm for 24 hours with external calibration), so even though a full plate (one set of 8 compounds yielding 96 samples) requires a 10 hour LC/MS run, the masses don't drift and the quantitation holds. I use a fast universal gradient LC method which has about a 6 minute cycle time. Since I have no time to even run survey samples, I simply look at the results as they come off, figure out which ion to quantitate on (almost always the M+H, but since my compounds are analyzed in mixtures it is sometimes necessary to quantitate on an isotope other than M+1), and then go from there. If a given compound yields for example M+Na instead, it doesn't matter, since the data was acquired in full scan mode, the decision on which ion to quantitate is made after the data is required. All the samples (except for the basals) have their own dilution series, so one just finds the apical (for example) which fits the linear part of the T0 curve, and then back calculate it's concentration. The basals, which are always the weakest, almost always fit the linear part of the curve, if they give a response at all (i.e.,if the compound is permeable). So this turns out to be a nice way to support discovery based ADME where you only have little (or in my case "no") time for optimization. Since a Qtof has essentially the same sensitivity in full-scan mode as it does when you are looking at single ion monitoring. Again my requirements are not as stringent as those would be in a development group. I typically have 5-10% RSDs and do not use a true internal std. We do put an additional compound in each well, just before MS analysis, and we monitor the reproducibility of this compound across the plate to look for injection errors, etc. I make use of the Sciex Turbo-Ionspray source which we have found to be fairly universal for the typical pharma like compounds we are analyzing. The new Sciex APCI source may approach this type of universiality, but it was not available for the Pulsar when we purchased it. We also have two LCTs in our group, but find these to be 5-10x less sensitive than the Pulsar (some combination of ion source and mass spec?). Also, the lower mass resolution and poorer mass stability of the LCT's means we have to open wider quantitation mass windows which increases the chemical noise. While, the Pulsar specs out to greater than 10,000 res at 1200, the resolution is less at lower masses. We typically (day to day operation) see greater than 8000 res at m/z 687 and 6500 or so for m/z 245. I typically have to re-tweek the tof parameters only about once every 1-3 months. There is no beating a triple when you have the time to optimize which parent-daughter (precusor-product if you want to be politically correct) pair you want to monitor. Also, it would be nice if you had time to optimize the LC conditions for each compound. However, none of these are an option for me. I haven't published any of this yet, since I'm a bit lazy and I'm pushing to many samples through the system right now. Ken Matuszak Research Investigator Abbott Laboratories D-4PN AP9A-L13 100 Abbott Park Road Abbott Park, IL 60064-6115 847-938-9225 (phone) 847-935-7929 (fax) ken.matuszak@abbott.com ed vd wal wrote: } I have a relative simple question: } just like Pete (APCI-TOF-MS) i am planning to set up a screening method. } I think about using a TOF for obvious reasons. However, can someone tell } me if the TOF's which are available now are quantitative? Or } what should i do to make it so? This is very importend for the method. } } Ed ****************************************************************************** From: Ken Matuszak Date: Wed, 06 Feb 2002 08:15:03 -0600 Subject: Re: screening with TOF-MS quantitative? Organization: Abbott Labs Pharmaceutical Products Division Ooops, see what happens when you type fast. The first line should have said "quantitative" and not "qualitative". Ken Matuszak wrote: } Ed: } } I use a Qstar Pulsar for qualititative work to support discovery based ADME } (currently permeability screens). There is no question that it's hard to } beat a triple for formal quantitation with their great dynamic range. } However, we have foound the Pulsar to be an excellent tool in our } application, because while the linear dynamic range is less than that of a } triple, we are able to make use of the high res of the Pulsar to operate in } full scan MS mode instead of MS/MS mode. } } For us, this is a key advantage, because we are pushing 28-42 new compounds } (which generates 4 to 6 - 96 well plates by the time we analyze replicates } of the basals, apicals, blanks, and T0s, and their dilution curves) through } one instrument with one operator each week. So, there is no time for } development. With this technique I have one generic MS setup (full scan 150 } to 1000) which is used for almost everything. The Pulsar's high resolving } power (10,000 at 1200), allows me to set pretty narrow mass ranges for } quantitation (0.1 amu or less), which effectively reduces the chemical } noise, in a way similiar to (maybe not as good as) a triple. With the res } of the Pulsar, the ion peak is well within the 0.1 amu peak width. The } Pulsar also has excellent mass stability (often I have 5 ppm for 24 hours } with external calibration), so even though a full plate (one set of 8 } compounds yielding 96 samples) requires a 10 hour LC/MS run, the masses } don't drift and the quantitation holds. I use a fast universal gradient LC } method which has about a 6 minute cycle time. } } Since I have no time to even run survey samples, I simply look at the } results as they come off, figure out which ion to quantitate on (almost } always the M+H, but since my compounds are analyzed in mixtures it is } sometimes necessary to quantitate on an isotope other than M+1), and then go } from there. If a given compound yields for example M+Na instead, it doesn't } matter, since the data was acquired in full scan mode, the decision on which } ion to quantitate is made after the data is required. } } All the samples (except for the basals) have their own dilution series, so } one just finds the apical (for example) which fits the linear part of the T0 } curve, and then back calculate it's concentration. The basals, which are } always the weakest, almost always fit the linear part of the curve, if they } give a response at all (i.e.,if the compound is permeable). } } So this turns out to be a nice way to support discovery based ADME where you } only have little (or in my case "no") time for optimization. Since a Qtof } has essentially the same sensitivity in full-scan mode as it does when you } are looking at single ion monitoring. Again my requirements are not as } stringent as those would be in a development group. I typically have 5-10% } RSDs and do not use a true internal std. We do put an additional compound } in each well, just before MS analysis, and we monitor the reproducibility of } this compound across the plate to look for injection errors, etc. } } I make use of the Sciex Turbo-Ionspray source which we have found to be } fairly universal for the typical pharma like compounds we are analyzing. } The new Sciex APCI source may approach this type of universiality, but it } was not available for the Pulsar when we purchased it. We also have two } LCTs in our group, but find these to be 5-10x less sensitive than the Pulsar } (some combination of ion source and mass spec?). Also, the lower mass } resolution and poorer mass stability of the LCT's means we have to open } wider quantitation mass windows which increases the chemical noise. While, } the Pulsar specs out to greater than 10,000 res at 1200, the resolution is } less at lower masses. We typically (day to day operation) see greater than } 8000 res at m/z 687 and 6500 or so for m/z 245. I typically have to } re-tweek the tof parameters only about once every 1-3 months. } } There is no beating a triple when you have the time to optimize which } parent-daughter (precusor-product if you want to be politically correct) } pair you want to monitor. Also, it would be nice if you had time to } optimize the LC conditions for each compound. However, none of these are an } option for me. } } I haven't published any of this yet, since I'm a bit lazy and I'm pushing to } many samples through the system right now. } } Ken Matuszak } Research Investigator } Abbott Laboratories } D-4PN AP9A-L13 } 100 Abbott Park Road } Abbott Park, IL 60064-6115 } 847-938-9225 (phone) } 847-935-7929 (fax) } ken.matuszak@abbott.com } } ed vd wal wrote: } } } I have a relative simple question: } } just like Pete (APCI-TOF-MS) i am planning to set up a screening method. } } I think about using a TOF for obvious reasons. However, can someone tell } } me if the TOF's which are available now are quantitative? Or } } what should i do to make it so? This is very importend for the method. } } } } Ed ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: screening with TOF-MS quantitative? Date: Wed, 6 Feb 2002 15:03:44 GMT Organization: JEOL USA, Inc. The limiting factor for most TOF's is not the microchannel plate (which has a high dynamic range) but the recovery time of the time-to-digital converter (TDC). There are alternative detectors. Chip Cody JEOL USA, Inc. In "chris.lee" writes: }With a TOF, the microchannel plate detector has a dead-time after the }arrival of each ion. As a result, the detector response (current per ion) }falls off at high ion counts, possibly at ion currents low enough for ion }statistics to still be a significant source of error. Having said that, TOFs }are OK for quantitative work if the software lets you know when the ion }current is within the acceptable range, and you're into trace rather than }high precision analyses. }This fundamental limitation of TOFs might come as a bit of a surpise if }you're used to other types of instrument, where linear dynamic range is less }"ed vd wal" a écrit dans le message news: }a3gsvh$3jj$1@news-int.gatech.edu... }} I have a relative simple question: }} just like Pete (APCI-TOF-MS) i am planning to set up a screening method. }} I think about using a TOF for obvious reasons. However, can someone tell }} me if the TOF's which are available now are quantitative? Or }} what should i do to make it so? This is very importend for the method. }} }} Ed }} }} }} -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ==============[ Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: "Steve Smith" Date: Wed, 6 Feb 2002 14:20:02 -0000 Subject: Micromass: New MassLynx updates available for download Organization: CompuServe Interactive Services Hi, The following MassLynx updates have recently been posted to the Micromass web site and are available for download via the links in this posting or from the MassLynx homepage ( http://www.micromass.co.uk/masslynx ). SCN 371 - MassLynx 3.5 for Q-Tof micro (details below) SCN 382 - MassLynx 3.5 for M@LDI (details below) SCN 412 - MassLynx 3.5 for Quattro Ultima with Ion Tunnel (details below) Waters 2488 MassLynx Control Software for MassLynx 3.5 (details below) SCN 1001i - MassLynx 3.6i for IsoPrime (details below) If you have any questions regarding these updates please contact your local software support department using the e-mail addresses supplied on our quick e-mail page. ( http://www.micromass.co.uk/email ) MassLynx 3.5 - Update 371 for Q-Tof micro - Fault Fixes From 3.5 * Cross talk in collision cell settings when performing TOF MSMS functions. This problem also manifests itself as inter function cross talk when running DDA experiments. * Using 'Use Tune Page Collision Energy' in the DDA MSMS template overrides all other collision energy preferences. * In DDA, if the number of components is greater than 1 and the number of precursors switched on is less than this number, then MSMS will occur for longer than specified. * Pusher width remains at 9 for all acquisitions. * Editing an MS or MSMS method results in an error dialog stating - 'Enter a valid number' * Centroid data is displayed at discrete mass values. * Running a sample list MS Method with a solvent delay in place can cause the system to fail * Change 'Temperature Correction' to DXC. * The polarity of the ion energy, pusher offset and steering references have been modified. * A new default.ipr has been included in the default project. * Software workaround for latching of quad in MSMS, and incorrect first scan time. * New default values for Hexapole program. ( http://www.micromass.co.uk/scn=371 ) MassLynx 3.5 - Update 382 for M@LDI - New Features * PAD software support. Fault Fixes From 3.5 * Range and default settings for MCP control have been amended. * Penning Trip defaults to correct value. * ADC default settings updated. * Dr. Watson errors reported while using SCN 367 have been fixed. * Summed shot / single shot data displays now function correctly. * De-isotope calibration option is re-enabled for reflectron mode on LR instruments. * Repaired the reflectron initialisation on starting an acquisition. * Repaired intermittent laser firing failure. ( http://www.micromass.co.uk/scn=382 ) MassLynx 3.5 - Update 412 for Quattro Ultima's with Ion Tunnels New Features * Minimum cone voltage is 35V. ( http://www.micromass.co.uk/scn=412 ) MassLynx 3.5 - Waters 2488 MassLynx Control Software. This update is available for download from the MassLynx 3.5 section of the web site along with Installation Instructions. (http://www.micromass.co.uk/masslynx/ChangeNotes/pupdates5.asp) MassLynx 3.6I - SCN 1001i for IsoPrime (Note - 3.6i is for Inorganic instruments only) New Features * Added new default references to Reference Library Fault Fixes From 3.6i * Fixed operation of HP6890 GC * Minor fixes to inlet scripts and default projects ( http://www.micromass.co.uk/scn=1001i ) Thanks for visiting our web site (http://www.micromass.co.uk) Note - If you register at the Micromass web site you can receive information about these updates via e-mail. Steve Smith Micromass UK ****************************************************************************** From: jon Date: Wed, 06 Feb 2002 20:36:02 +0000 Subject: calibrating/tuning a TSQ700 from scratch???? Organization: ntlworld News Service hi.... i just had to reinstall everything onto our TSQ700 decstation PC and now need to re-calibrate and tune the instrument for API use.. in GUIDE you can auto cali/tune the instrument but if it cant see the ion beam to begin with how can it calibrate? - im sure all that happened when i tried to calibrate it was it increased the multiplier voltage but nothing else..has anyone had any experience of doing this? the manual says a lot about EI/CI but not much on ESI/SPCI. Does anyone know a protocol for this ?? please help!!1 thanks jon. ****************************************************************************** From: "Mike Sherrell" Date: Wed, 6 Feb 2002 15:49:09 -0800 Subject: LC/Mass specs and MALDIs for sale Organization: * LC/Mass specs and MALDIs for sale: *Mass specs:. Micromass Ultima: $220,000. Asking price. (This instrument is not the Autospec). Micromass Q-Tof 1: $120,000. Installed. Sciex API 3000: $160,000. With Mac; price includes install and 30-day warr.; +$7K/NT data system upgrade. Sciex API 365: $60,000(!) Current NT software; guaranteed good working order. 10x sensitivity upgrade: $99,000. For API 365 or 300. Performed on-site; 1 year warr. Sciex API 150: $49,000. Includes E. Coast install. HP 1100. MSD: $55,000. Model A; esi + APCI. Bruker Esquire: $85,000. Includes ship, install, HP 1100. Ion trap; 1.5 years old. Finnigan LCQ Classic: $75,000. API, APCI, installed, 30-day warranty, 4000 amu upgrade. Sciex API III+: $30,000. Triple quad: ES, APCI; +$15K/intall w/ 1 yr. warr. Sciex API I: $20,000. Single quad; more sensitive than the Sciex 150. + $15,000/installation and 1 year service contract. Micromass LCZ Pltfrm: $40,000. ESI, APCI; +$5K/install, deliver, and 1 yr. service. MicroMass Quattro II: $195,000. Price includes installation & site license. Finnigan Navigator: $42,500. Guaranteed good working order. Finnigan TSQ 7000: $74,000. API 1: ES, APCI. Installation/$5,000. more. Finnigan SSQ 7000: $40,000. ES, APCI; Excalibur; API 1 source; install included. Finnigan TSQ 700: $25,000. Electrospray, APCI, Alpha workstn. ~$3K/install. Finnigan MAT 900: $75,000. Offers considered. EI, ES, APCI. MAT 95: $260,000. Hi res mag sector. 1994, ES, APCI, FAB, Thermospray, GC interface, install, delivery, 1 yr warr. AMD 604 S: $150,000. Double-focusing mag sector; very low detection limits, <4ppm mass, ESI/EI and FD sources. Install/service available. Offers condered. VG70E-HF: $20,000. Hi-res mag sector; EI/CI, FAB. Recent refurb. VG70-250SE: $12,500. Does not include SIOS/workstation. Many probes, many parts. Xtrell 400 ELQ: $20,000. Or best offer. LC/MS/MS, GC/MS/MS; 10 yrs. old, floor model, well-maintained. Fisons VG 2000: <$100,000. Fisons VG Trio: $25,000. LC + GC: 3000. amu; thermospray, EI/CI, HP 5890 included. Install, license & 90-day warr. +: $14,500. Kratos Concept: $7,500 + the price of removal. HP 5989: $21,500. Electrospray, APCI; 2000 amu. VG Trio 2: $7,500. Electrospray; complete; parts or fixer-upper unit. Nermag R10 10c: <$10,000. Like new; make offer. VG 7070-EHF small: $s 1984 model; used for FAB. Service and service contracts available for PESciex API 3000, 365 and III+. MALDI-TOFs: Micromass Q-TOF 1: $170,000. Incl. Waters Micro LC. Installation/warranty available. Bruker Biflex III: ~$50,000. DE; decommisioned by Bruker. LaserTec II: $75,000. By PerSeptive. 5 yrs. old; excellent condition. SRI custom design: $100,000. Asking price. 384 samples/20. min. Can be tested. Voyager LBT2: $20,000. ~1993. Excellent condition. Non-DE. Finnigan MAT Vision 2000. Reflectron: $80,000. Includes install, 1 year warranty Thermo/Finnigan Dynamo linear DE $39,000. Includes West Coast install, 90-day warranty. LaserMAT 2000: $24,000. New detector & laser; +$2,000/install; service available. VG Tof-Spec: $5,000. Or best offer. For parts; new laser card and other new boards. *ICP-MS: Finnigan MAT SOLA: $50,000. Asking price. 8 yrs. old; incl. GF, hydrides gen. *GC/MS/MS:. Finnigan GCQ: $35,000. 4 yrs. old; well-maintained; install/svc contract avail. VG Isogas SIRA small: $s Series II gas-isotope ratio mass spec: for parts. *Other mass specs, NMRs, DNA/protein sequencers & synthesizers available; check the website. All listed items subject to prior sale. Regards, Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: joerg.hau@rdls.nestle.com Date: Thu, 7 Feb 2002 11:46:01 +0100 Subject: Sensitivity problem on Quattro-LC Organization: * Dear All, Our Micromasss Quattro-LC is used in LC/MS with different kinds of samples. In december we have analysed some sulfonamides at the ppb level using MRM, and we got nice chromatograms with peak abundances in the 1e5 range. There were other priorities, then we returned to the sulfonamides. Using the same concentration of standards and identical working conditions, the signal is now almost two orders of magnitude lower (3e3...1e4 at best). Yes, we have done all the obvious: carefully compared our experimental records from december and today, and carefully re-tuned the MS on these compounds. No improvement. Yes, we have cleaned the cone, source and hexapole (formic acid), watched for obstrusion etc. Nothing unusual, all looks clean and perfect, e.g. tune is as good as it always was. We still have the impression that overall sensitivity has decreased a bit (but certainly not something like one order of magnitude). Still within factory specs, but somewhat worse than before. No, it isn't the standards, these are definitely stable. As a wild guess: We had a number of experiments done early this year (by another group), and they regularly had counts in the 1e6...1e7 range. Could an exposure to intermittent, high ion currents "blind" the detector (Quattro uses a photon-based detector, not a standard SEM)? Anyone experienced a similar phenomenon? Any other ideas what might be going on? Greetings from sunny Lausanne, - Joerg Joerg Hau Nestl=E9 Research Center PO Box 44, CH-1000 Lausanne 26 Phone + 41 21 785 8069 Fax + 41 21 785 8544 joerg.hau(at)dls.nestle.com ****************************************************************************** From: "Sumner, Lloyd" Date: Wed, 6 Feb 2002 17:04:01 -0600 Subject: Seeking a Edwards EXT-255 turbo pump Organization: * Dear All, I would like to purchase an Edwards EXT-255 (B7530-4000) turbo pump for a Bruker Esquire ion trap MS. (New, refurb, or used acceptable) Neither Bruker or Edwards have any pumps in stock. Anybody have a spare? Thanks Lloyd Lloyd W. Sumner, Ph.D. Staff Scietist & Head, Biological Mass Spectrometry The Noble Foundation 2510 Sam Noble Parkway Ardmore, OK 73401 Tele# 580-224-6710 Fax# 580-225-6692 Confidentiality Statement: The information contained in this message (and any attachment) may be confidential. If the reader is not the intended recipient, dissemination, copying, or using such information is prohibited. If you have received this communication in error, please notify us by replying to the sender's e-mail address or by telephone (580.223.5810) immediately. ****************************************************************************** From: leanne chen Date: Thu, 7 Feb 2002 06:06:41 -0800 (PST) Subject: Help Organization: * Hello, I am currently having a problem with ESI data and LC-ESI data. The ESI spectra has such peak cluster: ..., 717.5, 761.5, 805.6, 849.6, 893.6, 937.6, 981.6, ...,which obtained by infusing the sample into the MS on ESI mode directly. The LC-ESI spectra has another peak cluster: ..., 708.4, 752.4, 796.4, 840.4, 884.4, 928.4, ..., which obtained by using a C8 column and gradient LC program, the mobile phase includes CH3CN, H2O, and 1% acetic acid. My problem is why I got different spectra at both analysis method on a same sample. Can anyone help me to solve this puzzle please? Many thanks in advance. Leanna __________________________________________________ Do You Yahoo!? Send FREE Valentine eCards with Yahoo! Greetings! http://greetings.yahoo.com ****************************************************************************** From: Rodolphe Antoine Date: Thu, 07 Feb 2002 15:49:10 +0100 Subject: ESI-QMS Organization: CISM (Universite Claude Bernard Lyon I et INSA Lyon) Dear All, We have recently bougth an electrospray source from analytica of branford, and we have coupled this source to an extrel quadrupole mass spectrometer, however we would like to install between the ESI and the QMS, an hexapole ion guide. I saw that analytica of branford proposed the Iris(tm) hexapole Ion guide. Does anybody know where i can buy this guide ? Furthermore, I am looking for glass capillary (from analytica source) with metallized ends (with 6/10 mm or less inner diameter) Does anybody know where i can buy this kind of ESI parts ? Thanks, Rodolphe -- *************************************************************************** Dr. Rodolphe ANTOINE Chargé de recherche CNRS Laboratoire de Spectrométrie Ionique et Moléculaire UMR n° 5579 CNRS et Université Lyon1 Bât. Alfred KASTLER, Campus de la Doua 43, Bd du 11 novembre 1918 69622 Villeurbanne Cedex, France Bureau: (+33) 4 72 43 10 85 Télécopie: (+33) 4 72 43 15 07 *************************************************************************** ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Help Date: Thu, 7 Feb 2002 18:18:08 GMT Organization: JEOL USA, Inc. A mass difference of 44 always suggests a polyethoxylated species. If you infuse the sample, you are infusing sample plus other contaminants such as sodium, ammonium and so on. You may also be infusing similar species that may have different ability to compete for charge in the drying droplets. When you use LC sample introduction, you will be removing salts and separating other species that might interfere with each other. This is a very common problem, and it has led to some very creative approaches to sample cleanup prior to analysis. Sincerely, Chip Cody JEOL In leanne chen writes: }Hello, }I am currently having a problem with ESI data and }LC-ESI data. }The ESI spectra has such peak cluster: ..., 717.5, }761.5, 805.6, 849.6, 893.6, 937.6, 981.6, ...,which }obtained by infusing the sample into the MS on ESI }mode directly. }The LC-ESI spectra has another peak cluster: ..., }708.4, 752.4, 796.4, 840.4, 884.4, 928.4, ..., which }obtained by using a C8 column and gradient LC program, }the mobile phase includes CH3CN, H2O, and 1% acetic }acid. }My problem is why I got different spectra at both }analysis method on a same sample. Can anyone help me }to solve this puzzle please? }Many thanks in advance. }Leanna }__________________________________________________ }Do You Yahoo!? }Send FREE Valentine eCards with Yahoo! Greetings! }http://greetings.yahoo.com -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ==============[ Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: "Patrick Holland" Date: Fri, 8 Feb 2002 12:37:26 +1300 Subject: RE: Sensitivity problem on Quattro-LC Organization: * Optimal ESI sensitivity with the Z-spray source is critically dependent = on gas flow thru the Z block. We had a problem on our Quattro Ultima = after a clean of the interface innards which revolved around lack of an = O-ring in the interface. Check that you have all the right bits = correctly reassembled (see exploded diagram - not just the bits you have = as fitted by the factory) and then check on the effects of modifying the = flow with the restrictor screw. Is the sensitivity problem just with LC-MS of sulphonamides? What about = flow injection of other reference standards? Patrick T Holland PhD Biotoxins Section Cawthron Institute Private Bag 2 Nelson, New Zealand ph: 64 3 548 2319 FAX: 64 3 546 94 64 joerg.hau@rdls.nestle.com wrote: { {Dear All, {Our Micromasss Quattro-LC is used in LC/MS with different kinds of = {samples. In december we have analysed some sulfonamides at the ppb = level {using MRM, and we got nice chromatograms with peak abundances in = the 1e5 {range. {There were other priorities, then we returned to the sulfonamides. = Using {the same concentration of standards and identical working = conditions, {the signal is now almost two orders of magnitude lower = (3e3...1e4 at {best). {Yes, we have done all the obvious: carefully compared our experimental = {records from december and today, and carefully re-tuned the MS on these = {compounds. No improvement. Yes, we have cleaned the cone, source and = {hexapole (formic acid), watched for obstrusion etc. Nothing unusual, = all {looks clean and perfect, e.g. tune is as good as it always was. {We still have the impression that overall sensitivity has decreased a = {bit (but certainly not something like one order of magnitude). Still = {within factory specs, but somewhat worse than before. {No, it isn't the standards, these are definitely stable. {As a wild guess: We had a number of experiments done early this year = (by {another group), and they regularly had counts in the 1e6...1e7 = range. {Could an exposure to intermittent, high ion currents "blind" the = {detector (Quattro uses a photon-based detector, not a standard SEM)? {Anyone experienced a similar phenomenon? Any other ideas what might be = {going on? {Greetings from sunny Lausanne, {- Joerg ****************************************************************************** From: Christopher Kaine Date: Thu, 07 Feb 2002 21:00:15 -0500 Subject: Re: calibrating/tuning a TSQ700 from scratch???? Organization: Posted via Supernews, http://www.supernews.com Jon: Usually the default API tune will at least let you see the MRFA/MYO ions. If you can, I usually do a Course Calibration and set the pots for good peakshape. With a reload, the frequencies would have been set to some default and perhaps of no use. I would 'redip' the rods, save the frequencies, then do the Course Calibration. Hope the above helps. Do not forget you can call the ThermoFinnigan Technical Support Group@800-685-9535. Regards; Christopher Kaine Engineer MIDWEST ThermoFinnigan jon wrote: } hi.... } } i just had to reinstall everything onto our TSQ700 decstation PC and now } need to re-calibrate and tune the instrument for API use.. in GUIDE you } can auto cali/tune the instrument but if it cant see the ion beam to } begin with how can it calibrate? - im sure all that happened when i } tried to calibrate it was it increased the multiplier voltage but } nothing else..has anyone had any experience of doing this? the manual } says a lot about EI/CI but not much on ESI/SPCI. Does anyone know a } protocol for this ?? please help!!1 } } thanks } } jon. ****************************************************************************** From: msfiligenzi@ucdavis.edu (Mike Filigenzi) Date: 8 Feb 2002 11:17:44 -0800 Subject: Re: calibrating/tuning a TSQ700 from scratch???? Organization: http://groups.google.com/ jon wrote in message news:... } hi.... } } i just had to reinstall everything onto our TSQ700 decstation PC and now } need to re-calibrate and tune the instrument for API use.. in GUIDE you } can auto cali/tune the instrument but if it cant see the ion beam to } begin with how can it calibrate? - im sure all that happened when i } tried to calibrate it was it increased the multiplier voltage but } nothing else..has anyone had any experience of doing this? the manual } says a lot about EI/CI but not much on ESI/SPCI. Does anyone know a } protocol for this ?? please help!!1 } } thanks } } jon. Someone more knowledgeable than I am is likely to chime in, but in the meantime, have you checked your RF Tune? I would guess that reinstalling everything would wipe out the old RF Tune table and require a re-tune. Also, do you have an EI/CI source available? If so, you may want to put it in and tune using FC-43 or some such to get the quad parameters optimized before going back to APCI/ESI. Good luck! Mike ****************************************************************************** From: jon Date: Fri, 08 Feb 2002 19:53:44 +0000 Subject: Re: calibrating/tuning a TSQ700 from scratch???? Organization: ntlworld News Service yeh.. i needed to retune the rod rf values, and i found an old printout of some "good" voltages so I had a good startpoint..after that auto-tune took care of the rest! thanks for yor replies! Jon Mike Filigenzi wrote: } jon wrote in me