****************************************************************************** From: MURAT ERAVCI Date: Mon, 4 Jan 1999 15:51:24 +0100 Subject: ImageMaster 2D Software Organization: Freie Universitaet Berlin Who has got the ImageMaster 2D Software, or is already using it ? I would be very happy if you could contact me for further corrospondence. Thank You Murat Eravci My Email is eravci@zedat.fu-berlin.de ****************************************************************************** From: DIETER.ZIMMER.DZ@bayer-ag.de Date: Tue, 5 Jan 1999 08:57:31 +0100 Subject: Re: Selection of LCMS Organization: * Dr. Rao (Dubai) wrote }We are a Food Control Laboratory involved in the quality control analysis }of foods with emphasis on food contaminants such as veterinary drug }residues, pesticide residues, migration of packaging materials (additives }and monomers), mycotoxins etc. } }Recently we opened quotations for LCMS essentially for the confirmation }and quantitation of veterinary drug residues in meat (muscle, liver and }kidney) milk and eggs at the MRLs set by the Codex; pesticide residues in }different food matrices and packaging additives. The quotations received }are C.I.D. MS instruments (HP1100, Micromass LCZ, Perkin Elmer API 150), }ion-trap (Finnigan Mat LCQ) and Triple Quad (Micromass Quattro & Perkin }Elmer API 2000). After initial evaluation, we have decided to go for }higher range models (Quattro, PE 2000 and LCQ) which offer high }flexibility. I need your help in the selection of the right equipment }which can do the following: } } Confirmation of residues at low levels } Quantitation of residues in dirty samples with a gooddegree of } precision and accuracy. } High sample throughput capability for routine analysis and } withstand sensitivity loss } Technical advantages/draw backs, if any, of each equipment } } User friendliness, maintenance problems (we have limited } service backup) and maintenance costs } Your choice of equipment for the above work (LCQ or Triple } Quad & make) I am working with a pharmaceutical company in Pharmacokinetics. We are doing routine trace quantitation of new drugs out body fluids, like e.g. plasma & blood. We are using for this demanding work PE SCIEX API triple quad instruments since 1992 (API 3, API 300/365 and recently API 3000) Recently we did a comparison of the API 3000, 2000 and the Micromass Quattro II. For our application the API 3000 proved to be the best. The API 3000 is definitely more sensitive than the API 2000 and the Quattro. However, the price of the API 3000 is higher as well - if you can afford the money than it is better to buy the most sensitive instrument which is the API 3000 (today). Generally speaking: For quantitation you should definitely buy a triple quad. On conferences (and from one of my friends) it is reported that the LCQ is excellent for structure elucidation and other qualitative work, but if you need a rugged workhorse for daily routine quantitation a triple quad is the better choice than the LCQ. From the LCQ matrix effects are frequently reported thus users have to take more care to prepare cleaner samples, meanwhile the triple quads digest dirty samples to some extent. This is my personal opinion and does not reflect the opinion of my company. If you need more information, please feel free to contact me: Dr. Dieter Zimmer Bayer AG Preclinical Pharmacokinetics PF 10 17 09 42096 Wuppertal, Germany Phone + 49-202-36-4173 e-mail: Dieter.Zimmer.DZ@Bayer-Ag.de ****************************************************************************** From: eu001491 Date: Tue, 05 Jan 1999 16:18:10 +0100 Subject: What could be MS114 ? Organization: EUnet Belgium, Leuven, Belgium [The file attached to this post has been saved at the STMS FTP site, ftp.gatech.edu/pub/mass-spec, with the name MS114.jpg. DB] Good afternoon All, First, our apologies for cross-posting our question to three groups. Simply we desire to have a good cross-fertilisation of approaches to the following question: Can some of you inform us about the chemical formula, name, references, etc., of a rather strong smelling compound with the following mass spectra data? Molecular weight = 144 MS-data, with mass peaks at: 71 = 100% 99 = 88.5% 41 = 37.4% 69 = 35.1% 114 = 21.4% 42 = 17.6% 67 = 16.8% 53 = 14.5% 68 = 10.9% 54 = 10.7% Smelling this compound suggests a clinical- or medical-like odour reminding some sorts of desinfectant. Our first analysis suggests that it can contaminate some food products during transport, distribution, and/or storage at warehouses, most probably in some Middle-East Countries. The mass spectra has been scanned and saved as a MS114.jpg. See attachment. With anticipated thanks for your comments and kind regards, Luc -- Luc De Bry, Ph.D. Head of Research Department GENERAL BISCUITS BELGIE De Beukelaer-Pareinlaan 1 B-2200 Herentals Belgium Tel. 32 (0)14 24 14 32 Fax 32 (0)14 24 12 85 Email: luc.de.bry@pophost.eunet.be ****************************************************************************** From: Nick Wedd Date: Tue, 5 Jan 1999 16:31:46 +0000 Subject: Re: What could be MS114 ? Organization: wcc In article <76tckj$kps@acmey.gatech.edu>, eu001491 writes }Molecular weight = 144 Is that the uncharged mass? }MS-data, with mass peaks at: } }71 = 100% }99 = 88.5% }41 = 37.4% }69 = 35.1% }114 = 21.4% }42 = 17.6% }67 = 16.8% }53 = 14.5% }68 = 10.9% }54 = 10.7% Are these protonated masses? Are they all known to be singly-charged? Nick -- Nick Wedd nick@maproom.co.uk ****************************************************************************** From: "David Sparkman" Date: Tue, 05 Jan 1999 17:01:40 GMT Subject: Re: What could be MS114 ? Organization: @Home Network How about 1,6-dimethyl piperazine, C6H14N2, CAS RN 108-49-6? This compound has an NIST98 ID number of 10518 in the Main Database. The spectra are similar when you look at the NIST98 replicate spectrum ID number 6721. Regards; O. David Sparkman Consultant-At-Large ods@compuserve.com eu001491 wrote in message ****************************************************************************** From: "Neil Spingarn" Date: Tue, 5 Jan 1999 09:43:47 -0800 Subject: Re: What could be MS114 ? Organization: IBM.NET The identity doesn't jump out at me. First, are you SURE the MW is 144? How was this determined? Second, how did you get the MS data? Are you sure this is from a single component? Finally, if you give me (us) some background information it may provide some clues. The closest MS I could find was dimethyloctanone (MW 156). Try giving us more to work with. Neil eu001491 wrote in message <76tckj$kps@acmey.gatech.edu>... }[The file attached to this post has been saved at the STMS FTP site, }ftp.gatech.edu/pub/mass-spec, with the name MS114.jpg. DB] } } }Good afternoon All, } }First, our apologies for cross-posting our question to three groups. }Simply we desire to have a good cross-fertilisation of approaches to the }following question: } }Can some of you inform us about the chemical formula, name, references, }etc., of a rather strong smelling compound with the following mass }spectra data? } }Molecular weight = 144 } }MS-data, with mass peaks at: } }71 = 100% }99 = 88.5% }41 = 37.4% }69 = 35.1% }114 = 21.4% }42 = 17.6% }67 = 16.8% }53 = 14.5% }68 = 10.9% }54 = 10.7% } }Smelling this compound suggests a clinical- or medical-like odour }reminding some sorts of desinfectant. Our first analysis suggests that }it can contaminate some food products during transport, distribution, }and/or storage at warehouses, most probably in some Middle-East }Countries. } }The mass spectra has been scanned and saved as a MS114.jpg. See }attachment. } }With anticipated thanks for your comments and kind regards, } }Luc }-- }Luc De Bry, Ph.D. }Head of Research Department }GENERAL BISCUITS BELGIE }De Beukelaer-Pareinlaan 1 }B-2200 Herentals }Belgium }Tel. 32 (0)14 24 14 32 }Fax 32 (0)14 24 12 85 }Email: luc.de.bry@pophost.eunet.be } ****************************************************************************** From: gpj@austin.unimelb.edu.au Date: Wed, 06 Jan 1999 10:38:42 GMT Subject: Improvements to GCQ Organization: Deja News - The Leader in Internet Discussion As a follow up to my earlier announcement of a way to stabilise the sensitivity of the GCQ ion source, readers might like to know that I have finally cleaned the ion source after several months use, and this produced only a ca. 3-fold increase in spectrum intensity. We cleaned lens 1 earlier as this was not tuning as sharply as usual and this was sufficient to maintain it until now. While I hope to hear from others as to the effectiveness of my modification, I am convinced that the modification works. Graham P. Jackman -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ****************************************************************************** From: eu001491 Date: Wed, 06 Jan 1999 15:19:48 +0100 Subject: Re: What could be MS114 ? Organization: EUnet Belgium, Leuven, Belgium Sorry, there was a typing mistake : as in the title, the Molecular Weight is well 114, and not 144. from previous message: } [The file attached to this post has been saved at the STMS FTP site, } ftp.gatech.edu/pub/mass-spec, with the name MS114.jpg. DB] } } Good afternoon All, } } First, our apologies for cross-posting our question to three groups. } Simply we desire to have a good cross-fertilisation of approaches to the } following question: } } Can some of you inform us about the chemical formula, name, references, } etc., of a rather strong smelling compound with the following mass } spectra data? } } Molecular weight = 144 } } MS-data, with mass peaks at: } } 71 = 100% } 99 = 88.5% } 41 = 37.4% } 69 = 35.1% } 114 = 21.4% } 42 = 17.6% } 67 = 16.8% } 53 = 14.5% } 68 = 10.9% } 54 = 10.7% } } Smelling this compound suggests a clinical- or medical-like odour } reminding some sorts of desinfectant. Our first analysis suggests that } it can contaminate some food products during transport, distribution, } and/or storage at warehouses, most probably in some Middle-East } Countries. } } The mass spectra has been scanned and saved as a MS114.jpg. See } attachment. } } With anticipated thanks for your comments and kind regards, } } Luc } -- } Luc De Bry, Ph.D. } Head of Research Department } GENERAL BISCUITS BELGIE } De Beukelaer-Pareinlaan 1 } B-2200 Herentals } Belgium } Tel. 32 (0)14 24 14 32 } Fax 32 (0)14 24 12 85 } Email: luc.de.bry@pophost.eunet.be ****************************************************************************** From: Arkady_Gusev@RohmHaas.Com (Arkady Gusev) Date: Wed, 6 Jan 1999 16:15:11 -0500 Subject: Nitrogen source for LC/MS Organization: * Dear Colleges I would appreciate if somebody could comment or share some experience concerning using "house" nitrogen to run Sciex or Micromas LC/MS. Could you recommend specific gas filters to be used for on-line gas purification. Another question concerns formic acid. Could you recommend specific brand (lot#) which works best i.e. contamination, bottle-to-bottle quality, etc. We are using formic acid from Baker in glass bottles. Any comments? Sincerely Arkady Gusev ****************************************************************************** From: Fouad Haghseresht Date: Thu, 07 Jan 1999 14:30:34 +1000 Subject: Re: Gas Analysis Organization: University of Queensland Hi I have GC-MS on line with my experimental rig. In my set up, NO reacts with carbon and I need to measure the products which are CO, CO2, NO (unreacted) and N2. However, a column that can separate these compounds is difficult to find. I have so far found out that Chromosphere (I am not sure of the correct spelling) which is a 1/8 inch packed column can separate CO, CO2 and N2. I would love to hear from any one who can give me ideas of how I can successfully achieve quantitative analysis of these compounds. Are there any capilary columns that can do this? Thanks Fouad ****************************************************************************** From: moljaca@ccvd.com (Miodrag Oljaca) Date: Thu, 07 Jan 1999 03:54:51 GMT Subject: fluorescence measurements Organization: MindSpring Enterprises I am thinking about performing fluorescence (OH or CH) measurements in flames and would like to find out if I can use something like blue LED (at 450 nm) to excite molecules. I know that excitation is usually performed with the laser source. Is anyone familiar with use of blue LED or pulsed Xenon probes for excitation? We plan to use an OceanOptics spectrometer (S2000) to measure emission. Miodrag Oljaca MicroCoating Technologies ****************************************************************************** From: alarouche@aol.com (Alarouche) Date: 8 Jan 1999 00:00:14 GMT Subject: Calibration gas Organization: AOL http://www.aol.com Does any one know where I can view spectras of calibration gas used in GCMS in particular PFK and tributylamine. Thank-you ****************************************************************************** From: yossi.miller@agan.co.il (yossi miller) Date: Fri, 08 Jan 1999 10:18:06 GMT Subject: Re: Nitrogen source for LC/MS Organization: agan On 6 Jan 1999 16:54:45 -0500, Arkady_Gusev@RohmHaas.Com (Arkady Gusev) wrote: }Dear Colleges } }I would appreciate if somebody could comment or share some experience }concerning using "house" nitrogen to run Sciex or Micromas LC/MS. Could }you recommend specific gas filters to be used for on-line gas }purification. } }Another question concerns formic acid. Could you recommend specific brand }(lot#) which works best i.e. contamination, bottle-to-bottle quality, etc. }We are using formic acid from Baker in glass bottles. Any comments? We have the Sciex API 365 and use liquid nitrogen, it is consumed in (100 li container)3-4 weeks of lc/ms operation. We use Baker analysed formic acid and also acetic acid. We work one year and have no problems. Yossi Miller Agan Israel yossi.miller@agan.co.il } } }Arkady Gusev } ****************************************************************************** From: "David Sparkman" Date: Fri, 08 Jan 1999 14:03:00 GMT Subject: Re: Calibration gas Organization: @Home Network You can find mass spectra of PFK and PFTBA (perfluorotributylamine) in J. Throck Watson's "Introduction to Mass Spectrometry", 3rd ed., Lippincott-Raven, 1997, page 346. Spectra of these two substances can also be found in the NIST98 Mass Spectral Database and in course material for various ACS MS Short Courses taught by Watson and myself. Regards; O. David Sparkman Consultant-At-Large ods@compuserve.com Alarouche wrote in message <774sn1$8lo@acmex.gatech.edu>... }Does any one know where I can view spectras of calibration gas used in GCMS }in particular PFK and tributylamine. } }Thank-you } } ****************************************************************************** From: John Dulak Date: Fri, 08 Jan 1999 09:18:07 -0500 Subject: Re: Calibration gas Organization: Gnomeway Alarouche: Try the following URL: http://www.sisweb.com/index/referenc/mscalibr.htm John Dulak Alarouche wrote: } } Does any one know where I can view spectras of calibration gas used in GCMS } in particular PFK and tributylamine. } } Thank-you -- \\\\\// "Don't take life so seriously... | | ...it's only a temporary condition." (.) (.) ========================================oOO==(_)==OOo== _________________________ | John G. Dulak | /)| (IC&GLIC) |(\ / )| jdulak@ghplus.infi.net |( \ _( (|_________________________|) )_ ((( \ \ > /_) ( \ < / / ))) (\\\ \ \_/ / \ \_/ / ///) \ / \ / \ _/ \_ / / / \ \ / / \ \ ****************************************************************************** From: s.yates@ed.ac.uk Date: Fri, 08 Jan 1999 15:14:38 GMT Subject: Detector technology articles/books Organization: Deja News - The Leader in Internet Discussion Hello, Does anybody on this list know of any books or articles that descibe the theory and/or practice involved with mass detectors, such as MCPs. I am looking for articles that describe: the requirements for postive and negative ion detection; canonical and impedance matching etc. etc. A reference that might allow one to build a MCP mass detector would be about the right level. Thanks, Sandy -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ****************************************************************************** From: "Michael Schrader" Subject: MS: automated exchange of MALDI plates Date: Fri, 8 Jan 1999 16:41:02 +0100 Organization: * [This is from the ABRF mailing list. DB] We would like to increase the throughput of our PerSeptive STR MALDI-MS. Does anyone know about a solution which allows automatic exchange of the sample plate by a robotic arm for example? Please also reply if you heard about a solution for other instruments or manufacturer. Michael ---------------------------------------- Dr. Michael Schrader BioVisioN GmbH & Co. KG Feodor-Lynen-Str. 5 D-30625 Hannover GERMANY Phone +49-511-53 88 96-12 Fax +49-511-53 88 96-66 ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Calibration gas Date: Fri, 8 Jan 1999 19:13:00 GMT Organization: JEOL USA, Inc. In <774sn1$8lo@acmex.gatech.edu> alarouche@aol.com (Alarouche) writes: }Does any one know where I can view spectras of calibration gas used in GCMS }in particular PFK and tributylamine. }Thank-you Reference data are available on our web page (www.jeol.com). Follow the links to "Products"-->"Mass Spectrometry" and look for the list of masses for common calibation standards. The exact masses for fragment ions of both compounds are listed, and you can view the PFK mass spectrum. -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: mstoner713@aol.com (MStoner713) Date: 11 Jan 1999 03:19:41 GMT Subject: IR Spectra Organization: AOL http://www.aol.com I know that this newsgroup is dedicated to mass spectrometry, but I need help finding infrared spectra online. I am particularly interested in the spectra of phenol, salicylic acid, and acetalsalicylic acid. I have already looked in several organic texts, but I have not had any luck. Thanks, Mike ****************************************************************************** From: Werner Spahl Date: Mon, 11 Jan 1999 10:18:54 +0100 Subject: Urgent: MS80 spares to give away! Organization: [posted via] Leibniz-Rechenzentrum, Muenchen (Germany) Hi, we are dismantling an Kratos MS80 right now and would give away all the spare parts, all Probes (two DCI), a Carlo Erba GC and even the whole electronic cabinet if someone is interrested. Just send me an email or else they will just be thrown away... -- Werner Spahl (ui22273@sun1.lrz-muenchen.de) Freedom for "The meaning of my life is to make me crazy!" Vorlonships ****************************************************************************** From: cminvms@prism.gatech.edu (Mass Spec Lab) Date: 11 Jan 1999 07:49:10 -0500 Subject: Re: IR Spectra Organization: Georgia Institute of Technology MStoner713 wrote: }I know that this newsgroup is dedicated to mass spectrometry, but I need help }finding infrared spectra online. I am particularly interested in the spectra }of phenol, salicylic acid, and acetalsalicylic acid. Try NIST Chemistry Webbook for online mass and IR spectra plus a lot of other valuable information at http://webbook.nist.gov/chemistry/. This site has IR spectra for aspirin and salicylic acid. S.Shealy -- Mass Spectrometry Laboratory Georgia Institute of Technology, Atlanta Georgia, 30332 Internet: cminvms@acme.gatech.edu ****************************************************************************** From: David Sparkman Date: Mon, 11 Jan 1999 11:49:22 -0500 Subject: NEW GC/MS and LC/MS Stuff from NIST Organization: * GC/MS and LC/MS Users: The National Institute of Standards and Technology (NIST) has developed a powerful software companion for the NIST Mass Spectral Search Program for Windows and the NIST/EPA/NIH Mass Spectral Database of ~130K spectra (NIST98). This software is an Automated Mass spectral Deconvolution and Identification System (AMDIS). AMDIS is distributed at no extra charge with NIST98 and the NIST MSS Program v1.6. It is also available at the NIST Mass Spectral Data Center ftp site. A method to have both AMDIS and the NIST MSS Program share a split display so that both programs are viewable at the same time has been developed. Download AMDIS_NIST_MSS.DOC and SPLIT.INI from the NIST Mass Spectral Data Center ftp site (ftp://masdata.nist.gov). The INI file is a Desktop configuration for use with the NIST MSS Program and should be placed in the directory that contains the NIST MSS Program. The DOC file explains how the two programs work together. Because of the many features of these two programs, Scientific Instruments Services (SIS) will offer a course on their use at the 1999 ASMS Meeting in Dallas, Texas. Watch the SISWEB.COM site for future information on this course. O. David Sparkman Consultant-At-Large ods@compuserve.com ****************************************************************************** From: "James H. Duckworth" Date: Mon, 11 Jan 1999 17:43:24 -0500 Subject: Re: IR Spectra Organization: Galactic Industries Corp. We have a small database of spectra on-line on our web site. Go to http://www.galactic.com/galactic/_gala/data.htm and click on "Spectral Database" in the left frame. Just fill in the form to search the database. (Currently we only index on compound name, CAS number, and molecular formula.) There are more than just IR spectra in there too (some MS and NMR). Once you find what you want you can view it on-line and even download the spectrum in SPC file format. (If you need it, there's a free Win95/96/NT viewer for SPC files - click on "Galactic Data Viewer" in the left frame). I checked and we have the spectra for phenol and salicylic acid, but not acetalsalicylic acid in the database. Hope this helps. James Duckworth Galactic Industries Corp. jhd@galactic.com http:/www.galactic.com MStoner713 wrote in message <77cqa1$4vk@acmex.gatech.edu>... }I know that this newsgroup is dedicated to mass spectrometry, but I need help }finding infrared spectra online. I am particularly interested in the spectra }of phenol, salicylic acid, and acetalsalicylic acid. I have already looked in }several organic texts, but I have not had any luck. } }Thanks, Mike } } ****************************************************************************** From: "D. Wadlow" <%20inquiries@sensors-research.com> Date: Mon, 11 Jan 1999 21:24:39 GMT Subject: Web resources Organization: Sensors Research Consulting, Inc. A page of critically reviewed links related to specific science and engineering categories including: sensors and instrumentation, physics, space physics, biomedical, materials (including smart materials and biomaterials), units and conversion factors, standards organizations, research centers, patents, innovation, Internet buyer's guides. Emphasis on information content, quality, meaning stable and/or special interest links and 'core' links from which to initiate a 'cold' search with one or two derivative links as examples. Occasional manufacturer's links when examples are needed or a company site provides extra content. Institutions and Organizations Internet databases / specialized links pages Articles, referenced data or well-written and presented handbooks. The page now contains about 270 links (size - 62k mainly text). Check it out at: http://www.sensors-research.com/links.htm Link suggestions and exchanges for any of the categories welcomed. Also for your winter reading see a short article entitled "Tips and facts - Organizing R&D for Success" at: http://www.sensors-research.com/tips.htm Provided courtesy of........ Sensors Research Consulting, Inc. Consulting by agreement and online - physical sensors and measurements. http://www.sensors-research.com/ ****************************************************************************** From: clee751159@AOL.COM (CLee751159) Date: 11 Jan 1999 21:25:10 GMT Subject: Re: Nitrogen source for LC/MS Organization: AOL, http://www.aol.fr }}Dear Colleges }} }}I would appreciate if somebody could comment or share some experience }}concerning using "house" nitrogen to run Sciex or Micromas LC/MS. Could }}you recommend specific gas filters to be used for on-line gas }}purification. }} }}Another question concerns formic acid. Could you recommend specific brand }}(lot#) which works best i.e. contamination, bottle-to-bottle quality, etc. }}We are using formic acid from Baker in glass bottles. Any comments? } }We have the Sciex API 365 and use liquid nitrogen, }it is consumed in (100 li container)3-4 weeks of lc/ms operation. }We use Baker analysed formic acid and also acetic acid. }We work one year and have no problems. } }Yossi Miller }Agan Israel }yossi.miller@agan.co.il }} }} }}Arkady Gusev }} } } I suspect that success with nitrogen supplies has more to do with the plumbing than with the quality of the gas. Specialist plumbers don't always understand why you need high-purity plumbing for gas of quite ordinary quality. Our plumber used Locktite sealing compound, and this wreaked havoc. Fortunately, the contamination went away when all the screw joints were replaced (teflon tape is OK). In pre-LC/MS days, people tended to redistill all their acids and solvents for isolating peptides and other products, and for GC/MS. Nowadays, we don't have time., which is a pity because LC/MS is much more difficult than GC/MS in this respect. I used to distill the 76% azeotrope of formic acid, and this left a horrible residue in the flask, even with good starting material. Formic acid now seems to be only available as 100% grade - does anyone know if this is more or less stable than water-containing grades. Since it is an aldehyde it is bound to go off more or less rapidly, at least at room temperature. Possibly, instability is also a problem with acetonitrile (I don't really know). If it is, there is no point trying to buy pure grades; it would be better to invest in an automatic still. Regards ****************************************************************************** From: Werner Spahl Date: Tue, 12 Jan 1999 17:20:37 +0100 Subject: Varian GC 3400 for Carlo Erba GC Organization: [posted via] Leibniz-Rechenzentrum, Muenchen (Germany) Hi, we have a spare Carlo Erba Mega GC and would like to exchange it against an Varian GC 3400 to connect to our MAT90. Anybody? -- Werner Spahl (ui22273@sun1.lrz-muenchen.de) Freedom for "The meaning of my life is to make me crazy!" Vorlonships ****************************************************************************** From: "Dr. John M. Bland" Date: Tue, 12 Jan 1999 11:36:39 -0600 Subject: LCMS of hydrocarbons Organization: The United States Department of Agriculture Does anyone know of a MS source (FAB, APCI, etc) or technique that will identify unsaturated hydrocarbons by LCMS? ****************************************************************************** From: David Sparkman Date: Tue, 12 Jan 1999 12:59:24 -0500 Subject: Re:New GC/MS and LC/MS Stuff from NIST Organization: * The url in my previous was incorrect: The correct url is: ftp://msdata.nist.gov Sorry!! O. David Sparkman Consultant-At-Large ods@compuserve.com ****************************************************************************** From: "Dr. John M. Bland" Date: Tue, 12 Jan 1999 12:23:29 -0600 Subject: Re: LCMS of hydrocarbons Organization: The United States Department of Agriculture That should of been saturated hydrocarbons in the original message. }"Dr. John M. Bland" wrote: Does anyone know of a MS source (FAB, APCI, etc) or }technique that will identify unsaturated hydrocarbons by LCMS? ****************************************************************************** From: sumner@mail.chem.tamu.edu (Lloyd Sumner ) Date: Tue, 12 Jan 1999 16:14:17 -0600 Subject: GC of permanent and noble gases Organization: * Fouad, J&W Scientific sells a "GS-Molesieve", 30m x 0.52mm i.d. capillary column that we have successfully used to separate permanent and light hydrocarbon gases (J&W part#115-3632). Similar columns should also be available from any other chromatographic supplier such as Alltech, Supelco, etc... J&W Scientific 91 Blue Ravine Road Folsom, CA 95630-4714 Tele#916-985-7888 Best Regards, Lloyd Sumner } }Hi. }I have GC-MS on line with my experimental rig. In my set up, NO reacts }with carbon and I need to measure the products which are CO, CO2, NO }(unreacted) and N2. However, a column that can separate these compounds }is difficult to find. I have so far found out that Chromosphere (I am }not sure of the correct spelling) which is a 1/8 inch packed column can }separate CO, CO2 and N2. } }I would love to hear from any one who can give me ideas of how I can }successfully achieve quantitative analysis of these compounds. Are there }any capilary columns that can do this? } }Thanks }Fouad Lloyd W. Sumner, Ph.D. Associate Director, The Laboratory for Biological Mass Spectrometry Texas A&M University Department of Chemistry P.O. Box 30012 College Station, TX 77842-3012 Voice #409-845-8404 Fax #409-845-4719 Email Sumner@mail.chem.tamu.edu For more information see our webpage at http://www.chem.tamu.edu/services/ms/ For more information on The Center for Chemical Characterization and Analysis see: http://www.chem.tamu.edu/CCCA/ ****************************************************************************** From: clee751159@aol.com (CLee751159) Date: 13 Jan 1999 13:08:36 GMT Subject: Re: LCMS of hydrocarbons Organization: AOL, http://www.aol.fr The particle beam interface should give reasonable sensitivity with the sort of mobile phase you will be using. Unfortunately, you can no longer buy the interface, and no supplier seems to have taken advantage of recently-published improvements. Having said that, it may be worth repeating that GC is always best if GC can be made to work - you get far more practical plates than with LC. The problem here for some potential users is that currently available small mass spectrometers come with only one dedicated interface. An exception is the German firm AMD, whose equipment is interesting in more than one respect. Regards } }That should of been saturated hydrocarbons in the original message. } }}"Dr. John M. Bland" wrote: Does anyone know of a MS source (FAB, APCI, etc) }or }}technique that will identify unsaturated hydrocarbons by LCMS? } } ****************************************************************************** From: David Sparkman Date: Wed, 13 Jan 1999 09:58:17 -0500 Subject: NEW GC/MS and LC/MS Stuff from NIST Organization: * GC/MS and LC/MS Users: Due to some omitted information and an error in the url of my previous posting, I am reposting this announcement. Thanks for your indulgence. The National Institute of Standards and Technology (NIST) has developed a powerful software companion for the NIST Mass Spectral Search Program for Windows and the NIST/EPA/NIH Mass Spectral Database of ~130K spectra (NIST98). This software is an Automated Mass spectral Deconvolution and Identification System (AMDIS). AMDIS is distributed at no extra charge with NIST98 and the NIST MSS Program v1.6. It is also available at the NIST Mass Spectral Data Center ftp site. A method to have both AMDIS and the NIST MSS Program share a split display so that both programs are viewable at the same time has been developed. Download AMDIS_NIST_MSS.DOC and SPLIT.INI from the NIST Mass Spectral Data Center \download directory on the ftp site (ftp://msdata.nist.gov). The INI file is a Desktop configuration for use with the NIST MSS Program and should be placed in the directory that contains the NIST MSS Program. The DOC file explains how the two programs work together. Because of the many features of these two programs, Scientific Instruments Services (SIS) will offer a course on their use at the 1999 ASMS Meeting in Dallas, Texas. Watch the SISWEB.COM site for future information on this course. The ftp site is: ftp://msdata.nist.gov The directory is: \download If you are accessing with yor internet browser, type: ftp://msdata.nist.gov/download If you are using ftp software, do an anonymous login User ID: anonymous Password: guest@unknown Sorry for any confusing my earlier posting may have caused. O. David Sparkman Consultant-At-Large ods@compuserve.com ****************************************************************************** From: "Fowler, Audree" Subject: PROTEOMICS position at UCLA Date: Tue, 12 Jan 1999 14:25:29 -0800 Organization: UCLA [This is from the ABRF mailing list. DB] As a lot of you are aware I retire at the end of June. I am on the search committee for a position at UCLA. We will be meeting next week so I am going to paste the ad below. If you are interested please send me your name to present to the committee. The ad is in the January 15th Science. You will need to send in your application but I will try to get things started here. Audree V. Fowler, Ph.D. Dept. of Biol. Chemistry UCLA School of Medicine Los Angeles, CA 90095-1737 afowler@mednet.ucla.edu Faculty Position in Proteomics University of California, Los Angeles The School of Medicine and the College of Letters and Science at UCLA seek a candidate who will develop a vigorous and innovative research program in the general area of proteomics, protein chemistry and gene discovery, and who will oversee the establishment of a state-of-the-art mass spectrometry and protein sequencing facility. The appointment will be at the level of (Tenured) Associate or full Professor. Exceptional (tenure track) Assistant Professor candidates will also be considered. The appointment will be associated with the Departments of Biological Chemistry (School of Medicine) and Chemistry & Biochemistry (Letters and Science), as well as the UCLA-DOE Laboratory of Structural Biology and Molecular Medicine. Applications should include a curriculum vitae, a statement of research accomplishments, and future research plans. Please provide the names of three referees. Consideration of applications will begin March 15, 1999 and continue until the search is completed. Please send materials to Proteomics Search Committee, Department of Biological Chemistry, UCLA School of Medicine, Box 951737, Los Angeles, CA 90095. UCLA is an equal opportunity employer. ****************************************************************************** From: linzhi chen Date: Wed, 13 Jan 1999 12:38:55 -0600 Subject: LC/MS short course Organization: 3M Company Does anyone know any LC/MS short (4-5days) coursees (4-5days) offered in US? Thanks. Linzhi Chen 3M Pharmaceuticals ****************************************************************************** From: 915612@candseek.com Date: Wed, 13 Jan 1999 14:51:14 -0500 (EST) Subject: JOBOP Director of Medical Affairs Organization: * Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: We have an immediate opening for two Medical Doctors working in the Regulatory or Clinical Affairs Department as the central liaison between regulatory agencies(FDA)and clinicians using products in Phase IV clinical trials. Qualifications: The qualified candidates will possess an M.D. with a background in epidemiology and 3+ years experience in safety surveillance and adverse event reporting with HIV/AIDS and/or Oncology related drugs. We offer a competitive compensation & benefits package and a challenging work environment. Geographic Location of Position: Positions available in Boston and Southern California If you know anyone that might be interested, please forward this to them or contact: Frank Kraus Diedre Moire Corporation, Inc. Voice: (609) 584-9000 ext. 234 Fax: (609) 584-9575 Email: 915612@candseek.com ****************************************************************************** From: ss17@prism.gatech.edu (S.J.Shealy) Date: 13 Jan 1999 15:02:08 -0500 Subject: Re: LC/MS short course Organization: Georgia Institute of Technology }linzhi chen wrote: }Does anyone know any LC/MS short courses (4-5days) offered in }US? There are a couple of web sites which have a calendar of short courses including LC/MS courses: has a calendar of short courses including LC/MS courses offered by PACS (Professional Analytical and Consulting Services) (Click on "meetings") has a list of their LC/MS courses offered at the next ASMS meeting on June 12-13 S.Shealy ****************************************************************************** From: Michael Sherrell Date: Wed, 13 Jan 1999 14:45:19 -0800 Subject: Finnigan TSQ 700 Organization: * Finnigan TSQ 700, electrospray, for sale for $80,000. 1994 model; currently under PE service contract, NOT Y2K compliant. Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com ****************************************************************************** From: "Wison Z. Shou" Date: Wed, 13 Jan 1999 18:39:52 -0500 Subject: Re: LC/MS short course Organization: Georgia Institute of Technology, Atlanta GA, USA Check out http://www.lcms.com out, they have a comprehensive list of courses offered at various conferences under their "Training" section. The owner of the site, Chem-Space Associates, also does on-site training for LC/MS. linzhi chen wrote: } Does anyone know any LC/MS short (4-5days) coursees (4-5days) offered in } US? } Thanks. } } Linzhi Chen } 3M Pharmaceuticals ****************************************************************************** From: "John G. Nikelly" Date: Thu, 14 Jan 1999 11:47:50 -0500 Subject: Freezing/Thawing of DNA Organization: * Hello, Does anyone know of studies on the effect of freezing and thawing of biological samples, particularly DNA, as it relates to the mass spectra ( MALDI or ESI)of the samples(lit. citations). In particular, would like to know if anyone has noticed differences in spectra when their samples are frozen and then thawed before analysis. Thanks in advance, Darryl Davis Dept. of Chemistry U.S.P John Nikelly John G. Nikelly Chemistry Department University of the Sciences in Philadelphia 600 South 43rd Street Philadelphia Pa 19104 Phone: (215) 596 8848 Fax: (215) 895 1100 j.nikell@usip.edu ****************************************************************************** From: Arkady_Gusev@RohmHaas.Com (Arkady Gusev) Date: Thu, 14 Jan 1999 14:18:19 -0500 Subject: Post-column infusion/addition of internal standards Organization: * Dear Colleges I would appreciate if somebody could give me any references concerning post column addition of internal standards (IS) for residue quantification. We have recently tried different approaches for IS introduction i.e. surrogate (addition before extraction), volumetric (after extraction but before quantification) and post-column addition of IS. Although I am sure that post column addition has been used, I can not find any references. Sincerely Arkady Gusev ****************************************************************************** From: Racine Robert Date: Thu, 14 Jan 1999 23:34:22 +0100 Subject: seeking info about spectrophotometer , UV-VIS-NIR Organization: Club-Internet (France) Hello I'm seeking for information about spectrophotometer which presents all these characteristics: wavelengh width : from 200 to 1100 nm or more, double monochromator possible to add a magnetic stirre dispositif and thermal controller ( like pelletier system) important : can record quickly entire absorption spectrum in regular interval of time during kinetic experiments : for biochemistry kinetic. I heard information about Varian ( cary serie) or Perkin Elmer (Lambda serie), but any brand can suit. Could you tell me more or send web adress ? where I can buy it.... Really , I'm waiting for your help !!! Thanks and regards Bob ****************************************************************************** From: Ron Orlando Date: Thu, 14 Jan 1999 17:30:02 -0500 Subject: Atlanta Athens Mass Spectrometry Discussion Group meeting announcement Organization: * January 14, 1999 To: AAMSDG Members From: Ron Orlando Hope that everyone had a happy holiday season, but now it is time to get back to work. Our next meeting will be held in the auditorium of the Complex Carbohydrate Research Center (CCRC) on Thursday, February 4 at 7:00 PM. Directions to the CCRC follow. The primary speaker for the evening is Professor Craig Lunte (Department of Chemistry, University of Kansas) who will discuss microscale sample manipulation and CE interfacing with mass spectrometry. I will send the real title of Professor Lunte’s presentation in my next announcement. So, mark your calendars -- I am sure that you will want to attend. I would also like to ask for "less senior" volunteer to give the short presentation at this meeting. Please let me know if you have any questions regarding the upcoming meeting. We hope everyone will be able to attend. Ron Corporate Sponsor of AAMSDG: Bruker Instruments, Inc. Robert Adams 44 Manning Rd. Billerica, MA 01821 Telephone: (404) 255-4817 FAX: (404) 847-9892 E-mail: raadams@IX.NETCOM.COM PE Applied Biosystems David Griffin 850 Lincoln Centre Drive Foster City, CA 94404 Telephone: (800) 545-7547 Fax: (650) 572-2743 E-mail: GriffiDM@perkin-elmer.com Micromass, Inc. Tom Beaty 713 Brookfield Road Raleigh, NC 27615 Telephone: (919) 676-8467 E-mail: Tom_Beaty@MSPEOPLE.COM JEOL USA, Inc. Felix Salinas 11 Dearborn Rd. Peabody, MA 01961-6043 Telephone: (508) 535-5900 FAX: (508) 536-2205 E-mail: salinas@JEOL.COM ThermoQuest Corporation Randall L. Porch 450 Franklin Road, Suite 130 Marietta, GA 30067 Telephone: (770) 424-7880, (770) 671-8146 FAX: (770) 423-1114 E-mail: 103455.2414@compuserve.com Kratos Analytical, Inc. Brian K. Stall 100 Red Schoolhouse Rd. Building A Chestnut Ridge, NY 10977 Telephone: (914) 426-6700 FAX: (914) 426-6192 E-mail: bkstall@aol.com ------------------- Directions to the Complex Carbohydrate Research Center from Atlanta 1. Follow I-85 North to Highway 316 (Exit to Lawrenceville/Athens). 2. Follow Highway 316 until it merges with the Epps Bridge Road/Georgia Loop 10 exchange. You will take a right onto the entrance of Georgia Loop 10 (East - this entrance to Georgia Loop 10 is before you cross the bridge that goes over the loop). 3. Follow Georgia Loop 10 to the College Station Road exit. Take this exit. 4. Take a right onto College Station Road. 5. At the first traffic light, take a right onto Riverbend Road. 6. Take a direct right into the parking lot. CCRC will be the second building on your right (the first building is the Riverbend Research Laboratories). -------------------------- ___________________________________________________________________ Ron Orlando Complex Carbohydrate Research Center and Department of Biochem. and Mol. Bio., University of Georgia E-Mail: Orlando@ccrc.uga.edu Phone: (706) 542-4429 ___________________________________________________________________ ****************************************************************************** From: filigenzim@quanterra.com Date: Thu, 14 Jan 1999 23:39:59 GMT Subject: TSP-2 Probe needed Organization: Deja News - The Leader in Internet Discussion I recently had the thermospray probe for one of my Finnigan TSP-2 systems burn up on me. Finnigan no longer sells these and hasn't been able to help in repairing it. I was wondering if anyone out there might have one of these sitting around that they'd be willing to part with. If so, please e-mail me at filigenzim@quanterra.com or call at (916) 374-4399. Thanks in advance. Mike Filigenzi Tho only guy left doing thermospray?????? -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ****************************************************************************** From: "Dean D. Fetterolf" Date: Thu, 14 Jan 1999 20:22:29 -0500 Subject: Wanted: Experienced Liquid Chromatographer Organization: Verio Mid-Atlantic http://www.fbi.gov/employment/profrc.htm ****************************************************************************** From: Galletti Guido Date: Fri, 15 Jan 1999 13:20:45 +0100 Subject: MALDI-TOF-MS Organization: * Dear Friends, we are the analytical group of the Department of Chemistry at the University of Bologna, Italy. We plan to buy a MALDI-TOF-MS for the widest range of applications (natural and synthetic polymers, proteins, etc.). We are looking for an updated list of MALDI-TOF-MS manufacturers, mail addresses, and relevant prices. Any advice about problems or particular advantages in the use of any particular instrument are welcome. Thank you to everybody. Regards, Guido and Paola ____________________________________________________ Prof. Guido Galletti Analytical Group of Chromatography Mass Spectrometry Department of Chemistry "G. Ciamician" University of Bologna via F. Selmi 2 40126 Bologna Italy phone +39.51.259459 or 259580 fax +39.51.259456 http://www.unibo.it/analitica-gc-ms email galletti@ciam.unibo.it ****************************************************************************** From: 915612@candseek.com Date: Fri, 15 Jan 1999 07:24:52 -0500 (EST) Subject: JOBOP Biochemical Pharmacologist/Medicinal Chemist Organization: * I represent a rapidly growing, privately held drug discovery technology company, committed to the creation of proprietary assay systems and databases that facilitate the rapid development of biopharmaceuticals and agricultural chemicals. We have an immediate opening for a Biochemical Pharmacologist/Medicinal Chemist to join our team. You will join a research team producing genome-wide gene expression patterns for the evaluation of pharmaceutical and agricultural lead compounds. Qualifications The qualified candidate will possess a Ph.D. in Pharmacology, Medicinal Chemistry, or a related field, plus 2-5 years postdoctoral experience (preferably in the pharmaceutical industry) is required. The candidate will also possess a strong knowledge of drug metabolism and mechanism of action. Experience in the isolation and identification of metabolites is desirable. We offer competitive compensation & benefits package, stock options and a challenging work environment. Geographic Location of Position: San Fransisco Bay Area,CA If you know anyone that might be interested, please forward this to them or contact: Frank Kraus Diedre Moire Corporation, Inc. Voice: (609) 584-9000 ext. 234 Fax: (609) 584-9575 Email: 915612@candseek.com ****************************************************************************** Date: Thu, 14 Jan 1999 15:42:49 -0500 From: lennon Subject: Mass Spec position available Organization: * [This is from the ABRF mailing list. DB] Research Assistant - Mass Spectrometry Participate in the discovery of molecular changes associated with disease and pharmaceutical effects in an R&D biotech company internationally known for pioneering work in proteomics. Successful candidate has a BS in chemistry, biology or related field, at least 1 years' experience in mass spectrometry (MALDI preferred) and familiarity with Windows NT and MS Office. Primary responsibilities include sample preparation, MS analysis of proteins separated via proprietary 2-D gel and other methods and maintenance of good laboratory records. Results are generated for pharma and other clients and for inclusion in the Company's growing databases of protein expression information. Excellent work environment. Salary commensurate with background and experience. Send letter with resume, salary requirements and references to Large Scale Biology Corp., 9620 Medical Center Dr., Suite 201, Rockville, MD 20850 or to personnel@lsbc.com (website http://www.lsbc.com). No faxes or phone calls, please. ****************************************************************************** From: David Bostwick Date: Fri, 15 Jan 1999 11:02:45 -0500 Subject: ASMS information Organization: * ASMS has sent us a file that combines the information from several of their links into one document. It contains the Conference schedule highlights, information about the short courses and accomodations, and registration forms for the Conference and short courses. The file is in Word format, and can be reached from our Web page at , from our FTP site at , or by anonymous FTP from the FTP site. David Bostwick ****************************************************************************** From: Date: Sat, 16 Jan 1999 00:55:28 +0100 Subject: Optimization software for the laboratory - free 30 day trial Organization: Telenordia Welcome to visit http://www.multisimplex.com to download the MultiSimplex software for sequential experimental design and optimization. Successful applications can be found in most branches of analytical chemistry and experimental laboratory work. Regards, Tomas Oberg MultiSimplex AB ****************************************************************************** From: "Wing Kwan" Date: Sat, 16 Jan 1999 16:12:58 +0300 Subject: ELAN 6000 ICP-MS SPECTROMETER FLOODED BY OIL FROM ROUGHING PUMP Organization: Saudi Aramco Hello, I am posting this to see whether there is some other users having the same problem. We are using an ELAN 6000 ICP-MS in our analytical laboratory, 14 months ago we detected that one of the roughing pump was leaking oil so we pulled the roughing pumps (two of them) out for rebuilding with the manufacturer supplied re-build kits. The rebuilding was uneventful and the pumps worked beautifully. Three or four months after the rebuilding, disaster struck, oil from one of the roughing pump was sucked into the spectrometer and ruined the turbo-molecular pump and some other electronic parts. After cleaning and rebuilding the spectrometer, we continue to test the instrument. After operating for two or three months, the same happened again, now the two turbo molecular pumps and the spectrometer are soaked with pump oil. I appreciate your help if any of the same instrument users had these happened, and know why. I also like to know if the turbo pumps are still usable after cleaning of the oil out. Please contact me at kwanwn@aramco.com.sa or post information in the news group. Thanks ****************************************************************************** From: Gianluca Giorgi Date: Sat, 16 Jan 1999 17:52:53 +0100 Subject: Meeting Announcement Organization: * Dear Sirs: the international meeting "3° MS-PHARMADAY-MASSA 99" will be held in Siena (Italy) on June 1-4, 1999. It will be focused on scientific aspects, applications and instrumentation that make mass spectrometry a versatile approach, often unique, to deal with a wide variety of problems. A special session will be devoted to mass spectrometry in the biopharmaceutical, biotechnological and biological sciences. Organized in invited lectures, oral communications and poster sessions, the meeting constitutes a useful occasion for updating on the growing developments and rapid evolution of mass spectrometry in these fields. Authoritative Italian and foreign scientists coming from both Universities and pharmaceutical companies will present the current state-of-the-art. Topics include: * MS tools for structural characterization of unknown compounds; * MS analysis and screening by combinatorial chemistry; * Gas-phase ion chemistry; * Recent developments in coupling with separative techniques; * New MS approaches in pharmacology and pharmacokinetics; * Mass spectrometry in quality control processes; * GLP protocols; * New developments in instrumentation and techniques. Further information can be found at http://www.cineca.it/hosted/mass_spectrom/3MS-PH/ or contact myself: Gianluca Giorgi C.I.A.D.S. - University of Siena via Aldo Moro - 53100 Siena (Italy) Tel. +39-0577-227135 Fax +39-0577-227137 E-mail: CIADS@UNISI.IT ____________________________________________________________________________ Gianluca GIORGI Centro Interdipartimentale di Analisi e Determinazioni Strutturali Università di Siena via A. Moro I-53100 SIENA (Italy) Tel. +39-0577-227135 Fax. +39-0577-227137 E-mail: ciads@unisi.it Internet: http://www.cineca.it/hosted/mass_spectrom/ggiorgi.htm ****************************************************************************** From: bowens8888@aol.com (BOwens8888) Date: 16 Jan 1999 21:37:22 GMT Subject: Re: ELAN 6000 ICP-MS SPECTROMETER FLOODED BY OIL FROM ROUGHING PUMP Organization: AOL http://www.aol.com Sounds like PE engineering! ****************************************************************************** From: Yining Zhao Date: Sun, 17 Jan 1999 14:20:39 +0100 (MET) Subject: polysulfatedsaccharides Organization: * Hi, Gentlemen: I'm tryin to get good sensitivity of a polysulfated polysaccharide sodium salt (MW 1726)in negative ESI mode. However, it barely gave the multiple-charged signals due to its very strong sodium affinity. I've used high content of TEA, Imidazole etc. such basic addutives in my makeup liquid, but the spectrum is still unsatisfied. Will you give me your experiences with such sort of compounds ? thanks in advance ----------------------------------- ****************************************************************************** From: Tony Davies Date: Sun, 17 Jan 1999 16:32:02 +0100 Subject: Re: NEW GC/MS and LC/MS Stuff from NIST Organization: * David Sparkman wrote: } GC/MS and LC/MS Users: } } The National Institute of Standards and Technology (NIST) has developed a } powerful software companion for the NIST Mass Spectral Search Program for } Windows and the NIST/EPA/NIH Mass Spectral Database of ~130K spectra } (NIST98). This software is an Automated Mass spectral Deconvolution and } Identification System (AMDIS). AMDIS is distributed at no extra charge } with NIST98 and the NIST MSS Program v1.6. It is also available at the } NIST Mass Spectral Data Center ftp site. } } A method to have both AMDIS and the NIST MSS Program share a split display } so that both programs are viewable at the same time has been developed. } Download AMDIS_NIST_MSS.DOC and SPLIT.INI from the NIST Mass Spectral Data } Center ftp site (ftp://masdata.nist.gov). The INI file is a Desktop } configuration for use with the NIST MSS Program and should be placed in } the directory that contains the NIST MSS Program. The DOC file explains } how the two programs work together. } } Because of the many features of these two programs, Scientific Instruments } Services (SIS) will offer a course on their use at the 1999 ASMS Meeting } in Dallas, Texas. Watch the SISWEB.COM site for future information on } this course. } } O. David Sparkman } Consultant-At-Large } ods@compuserve.com For an article on the AMDIS system see: http://www.spectroscopyeurope.com/td_col.html Tony Davies Spectroscopist at home (well it is 11:20 pm here!) ****************************************************************************** From: Eric_Block@waters.com Date: Mon, 18 Jan 1999 10:53:02 -0500 Subject: Particle beam interface Organization: * C. Lee wrote: ;The particle beam interface should give reasonable sensitivity with the ;sort of mobile phase you will be using. Unfortunately, you can no longer ;buy the interface, and no supplier seems to have taken advantage of ;recently-published improvements. I would call your attention to the "thermabeam" LC-MS system which is offered by Waters. This is a benchtop LC-MS system with a particle-beam interface which includes several of the improvements which Dr. Lee mentioned. This design avoids many of the problems encountered with earlier particle-beam interfaces. For more information, please visit the Waters web site at www.waters.com Eric Block Senior Applications Chemist Waters Corporation ****************************************************************************** From: tthomas@tek9.com Date: Mon, 18 Jan 1999 1:32:01 PM Subject: ASMS abstract deadline Organization: * 47th ASMS Conference on Mass Spectrometry June 13 - 17, 1999 The Wyndham Anatole Hotel, Dallas, Texas The deadline for receipt of abstracts for the conference is January 22. Submission is through the ASMS web page: http://www.asms.org Because of the very large number of abstracts expected (over 1600) we fear a log jam on January 22. We urge you to submit before the deadline to assure that your abstract gets through. If you do not receive an acknowledgement via e-mail, please check on whether your abstract was successfully transmitted by contacting ASMS AFTER January 27 by email (asms@asms.org) or inquire by fax: 505 989 1073. Thank you for your cooperation in submitting your abstract BEFORE January 22. ****************************************************************************** From: sxc59@po.cwru.edu Date: Tue, 19 Jan 99 14:50:33 GMT Subject: MALDI Modification Organization: * [This is forwarded from sci.chem.analytical. DB] Dear MALDI users, I have a bioperseptive voyager biospectrometry workstation. This is a linear type system and I am looking for ways/suggestions to convert it to a MALDI with delayed extraction. I appreciate any input, and apologize for posting to the wrong newsgroup. Sincerely Shu Chen sxc59@po.cwru.edu ****************************************************************************** From: 915608@candseek.com Date: Tue, 19 Jan 1999 12:24:04 -0500 (EST) Subject: JOBOP SCIENTIST COMPUTATIONAL CHEMIST Organization: * Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: A Scientist to work in close collaboration with scientists in Medicinal Chemistry and Biological Sciences Departments, applying computational chemistry techniques and combinatorial library design methods to drug design and discovery. The candidate will possess a Ph.D. in Chemistry or Biochemistry with relevant experience in computational chemistry, biochemistry, principles of drug design, x-ray crystallography techniques, QSAR, and medicinal chemistry. Previous experience with library design and development of methodology for library design. Expertise in the use of the UNIX operating system and programming languages used in the UNIX environment would be required. Our client is a leading pharmaceutical firm with research facilities in North Carolina and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: US-North Carolina If you know anyone that might be interested, please forward this to them or contact: Sam Strong DMC Fax: 609-584-9575 Email: 915608@candseek.com ****************************************************************************** From: William Cotham Date: Tue, 19 Jan 1999 13:16:47 -0500 Subject: Re: ELAN 6000 ICP-MS SPECTROMETER FLOODED BY OIL FROM ROUGHING PUMP Organization: Univ. of South Carolina, Columbia More information would be helpful -was there a power failure? -was there a catastrophic vacuum vent? It is very unusual for a rough pump to back stream oil into the mass spectrometer without something major going wrong. -- William E. Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry & Biochemistry University of South Carolina Columbia, SC 29208 Phone: (803) 777-2039 FAX : (803) 777-9521 ****************************************************************************** From: William Cotham Date: Tue, 19 Jan 1999 13:20:02 -0500 Subject: Re: polysulfatedsaccharides Organization: Univ. of South Carolina, Columbia A molecule that small will probably not want to hold multiple charges. I would expect the singly charged anion to be most abundant. Yining Zhao wrote: } } Hi, Gentlemen: } } I'm tryin to get good sensitivity of a polysulfated polysaccharide sodium } salt (MW 1726)in negative ESI mode. However, it barely gave the } multiple-charged signals due to its very strong sodium affinity. I've used } high content of TEA, Imidazole etc. such basic addutives in my makeup } liquid, but the spectrum is still unsatisfied. } } Will you give me your experiences with such sort of compounds ? thanks in } advance } } ----------------------------------- -- William E. Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry & Biochemistry University of South Carolina Columbia, SC 29208 Phone: (803) 777-2039 FAX : (803) 777-9521 ****************************************************************************** From: Peter Jacobs +31 412 661688 Date: Wed, 20 Jan 1999 10:00:01 +0000 (GMT) Subject: RE: polysulfated saccharides Organization: * Yining Zhao wrote: }I'm tryin to get good sensitivity of a polysulfated polysaccharide sodium }salt (MW 1726)in negative ESI mode. However, it barely gave the }multiple-charged signals due to its very strong sodium affinity. I've used }high content of TEA, Imidazole etc. such basic addutives in my makeup }liquid, but the spectrum is still unsatisfied. }Will you give me your experiences with such sort of compounds ? thanks in }advance With ESI a Dionex suppressor might help (or Dowex desalting); with MALDI the addition of basic (RG)n peptides as described originally by Peter Juhasz. ASMS abstracts on these subjects: Palm Springs, 1997 p1033 (MALDI) Portland, 1996 p565 (Ionspray) For an overview of ms approaches read the article below and references therein: Application of mass spectrometry to the analysis of natural and synthetic sulfated oligosaccharides glycosaminoglycans L. Silvestro, S. Rizea Savu, P.A. van Veelen and P.L. Jacobs - In: "Non-anticoagulant Actions of Glycosaminoglycans" Ed. Prof. J. Harenberg and B. Casu, eds., Plenum Press, New York, 1996 ISBN 0-306-45299-5 Peter Jacobs E-mail: p.jacobs@organon.oss.akzonobel.nl ****************************************************************************** From: Thomas Storm Date: Wed, 20 Jan 1999 09:36:30 +0100 Subject: Re: polysulfated saccharides Organization: TU-Berlin Yinning Zhao wrote: } Hi, Gentlemen: } } I'm tryin to get good sensitivity of a polysulfated polysaccharide sodium } salt (MW 1726)in negative ESI mode. However, it barely gave the } multiple-charged signals due to its very strong sodium affinity. I've used } high content of TEA, Imidazole etc. such basic addutives in my makeup } liquid, but the spectrum is still unsatisfied. } } Will you give me your experiences with such sort of compounds ? thanks in } advance } Hello, try removing sodium by a strong cation exchanger if your analyte survives a acidic environment. Did work with a polysulfonated polymer samples I once measured in negative ESI. Best Thomas -- Thomas Storm TU-Berlin Fachgebiet Wasserreinhaltung Sekr. KF4 Strasse des 17.Juni 135 10623 Berlin Tel. ++49 (0) 30 314 25480 FAX ++49 (0) 30 314 23850 e-mail: storm@itu203.ut.tu-berlin.de ****************************************************************************** From: Tom Crabtree Date: Wed, 20 Jan 1999 08:22:25 -0800 Subject: Re: ELAN 6000 ICP-MS SPECTROMETER FLOODED BY OIL FROM ROUGHING PUMP Organization: GB Scientific, Inc. Sir: The most likely scenario for back streaming roughing pump oil into your vacuum system is power failure. If your instrument experienced an unexpected power interruption while at high vacuum it not unreasonable for mechanical pump oil to be sucked up into the turbo pumps. I recommend that you install vacuum protection isolation valves on your roughing pumps. Two vendors that come to mind are Edwards High Vacuum and HPS. . They both offer valves that can be placed between the roughing pump and the high vacuum system that shut when the power to the roughing pump is shut off. This will prevent your system from getting contaminated. Your turbo pumps should be ok to used after you clean out all of the oil, but the only sure test is to turn them on. Good luck! GB Scientific, Inc. Tom Crabtree gbsci@gbscientific.com 415-898-7606 415-898-7514 (FAX) Wing Kwan wrote: } Hello, } } I am posting this to see whether there is some other users having the same } problem. } We are using an ELAN 6000 ICP-MS in our analytical laboratory, 14 months ago } we detected that one of the roughing pump was leaking oil so we pulled the } roughing pumps (two of them) out for rebuilding with the manufacturer } supplied re-build kits. The rebuilding was uneventful and the pumps worked } beautifully. Three or four months after the rebuilding, disaster struck, } oil from one of the roughing pump was sucked into the spectrometer and } ruined the turbo-molecular pump and some other electronic parts. } } After cleaning and rebuilding the spectrometer, we continue to test the } instrument. After operating for two or three months, the same happened } again, now the two turbo molecular pumps and the spectrometer are soaked } with pump oil. } } I appreciate your help if any of the same instrument users had these } happened, and know why. I also like to know if the turbo pumps are still } usable after cleaning of the oil out. } } Please contact me at kwanwn@aramco.com.sa or post information in the news } group. } } Thanks ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: polysulfatedsaccharides Date: Wed, 20 Jan 1999 20:41:37 GMT Organization: JEOL USA, Inc. In <782jlv$fa2@acmez.gatech.edu> William Cotham writes: }A molecule that small will probably not want to hold multiple charges. }I would expect the singly charged anion to be most abundant. In my experience, multiple charges are often observed for molecules in this molecular weight range. However, the degree of charging can be concentration-dependent as well as solvent-dependent. You might try a more a more dilute solution, and beware of salty solvents and glass containers. HPLC-grade purity is not a good guarantee that water is sodium-free (HPLC-grade often is only an indication that UV absorbers areabsent). Use a good quality water purification system if you have not already done so. No doubt other readers of this newsgroup will have additional suggestions to offer. Good luck! }Yining Zhao wrote: }} }} Hi, Gentlemen: }} }} I'm tryin to get good sensitivity of a polysulfated polysaccharide sodium }} salt (MW 1726)in negative ESI mode. However, it barely gave the }} multiple-charged signals due to its very strong sodium affinity. I've used }} high content of TEA, Imidazole etc. such basic addutives in my makeup }} liquid, but the spectrum is still unsatisfied. }} }} Will you give me your experiences with such sort of compounds ? thanks in }} advance }} }} ----------------------------------- -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: Peter Jacobs Date: Wed, 20 Jan 1999 23:04:56 +0100 Subject: 35th Anniversary of the Dutch Society for Mass Spectrometry (NVMS) Organization: XS4ALL, networking for the masses The 35th Anniversary of the Dutch Society for Mass Spectrometry will be celebrated with a two day Symposium organised at 'Congrescentrum De Reehorst', Bennekomseweg 24, Ede, The Netherlands, on March 17-18, 1999. 'De Reehorst' has ample parking space and is conveniently located next to the railway station in Ede. The town of Ede can be found on the A12/E35 between Arnhem (11 km) and Utrecht (85 km). Plenary Lectures: H.C. Meuzelaar (Univ. of Utah) "Have Mass Spectrometer, Will Travel" J.J. Boon (FOM-AMOLF) "Mass Spectrometric Examination of Paintings" M. Mann (Univ. of Southern Denmark) "Mass Spectrometry in Genomics/Proteomics" N.N.M. Nibbering (Univ. of Amsterdam) "Old and Recent Examples of Chemical Research with Use of Mass Spectrometry" J. Henion (Cornell Univ.) "From DLI to Ionspray and LC/MS/MS: an LC/MS Journey" J. van der Greef (TNO Pharma) "Mass Spectrometry: Key-technology in Bio-Pharmaceutical Analysis" F. Hillenkamp (Univ. of Münster) "MALDI in the Infrared: Physically Challenging but Useful Applications" M.C. ten Noever de Brauw "Dutch Mass Spectrometry Revisited" During this meeting also (2 hour) workshops will be organised by these manufacturers: Bruker, Hewlett-Packard, Micromass / Waters, Perkin-Elmer,Physical Electronics and Shimadzu. Furthermore (2 hour) tutorials on LC-MS (Chair: J. Henion / A. Bruins), MS/MS (Chair K. Vekey) and MALDI (Chair: F. Hillenkamp) will be held. Last but not least a poster session and a small exhibition (shortly after PittCon!) will be held. More detailed info, including the registration form, can be found at: http://www.xs4all.nl/~pjacobs/nvms.html Important dates: Abstracts for posters: February 12, 1999 Registration at reduced fee: February 1, 1999 Any questions? Contact Peter Jacobs Secretary of the Dutch Society for Mass Spectrometry E-mail: p.jacobs@organon.oss.akzonobel.nl ****************************************************************************** From: 915613@candseek.com Date: Thu, 21 Jan 1999 16:51:48 -0500 (EST) Subject: JOBOP ANALYTICAL/PK CHEMIST PHARMACEUTICAL RESEARCH Organization: * Since your email address was listed on a related web site page or database, I thought you might help. I am seeking an individual within the following conditions: An analytical chemist who will design and implement discovery support programs in the area of Drug Metabolism and Pharmacokinetics utilizing skills in bioanalysis (especially LC/MS), pharmacokinetics, and drug metabolism. The qualified candidate will also develop bioanalytical methodology, perform and oversee in vivo pharmacokinetic and in-vitro metabolism studies that support discovery and development programs. The candidate should possess a Ph.D. with post doctoral experience and at least two years industry experience. Our client is a leading biotech firm with research facilities in Baltimore, Maryland and can provide excellent benefits (health insurance, dental, and vision plan, paid vacation and more). A high impact, high profile position with excellent opportunity for advancement. Geographic Location of Position: Maryland If you know anyone that might be interested, please forward this to them or contact: Barney Giannone DMC Voice: 609-584-9000x235 Fax: 609-584-9575 Email: 915613@candseek.com ****************************************************************************** From: hionsolar@aol.com (Hion solar) Date: 22 Jan 1999 04:37:32 GMT Subject: Wanted: software to take data from CVC Quad1210A ResidGasAnalyzer Organization: AOL http://www.aol.com Does anyone know where I can get software to pick off peaks and give partial pressures and masses of my product gas mixture [H2 + O2+H20] using residual gas analyzer? Thanks and Regards.....Walt Pyle ****************************************************************************** From: david.bostwick@chemistry.gatech.edu (David Bostwick) Date: Fri, 22 Jan 99 20:03:00 GMT Subject: Information request Organization: Georgia Institute of Technology, Atlanta GA, USA We would like to learn more about the coverage of the newsgroup and the remailer that John Bartmess runs. One of the hopes in creating the newsgroup was that it would be of interest to more than just the members of the American Society for Mass Spectrometry or other mass spectrometry societies around the world. To estimate the coverage of the newsgroup, we would like to collect some data. If you would like to participate, please reply to this with the following information: 1. Do you read the newsgroup directly or are you subscribed to John's remailer? 2. Are you a member of the ASMS or another mass spectrometry society? The only information saved will be the country of origin of each message, and the answers to the two questions above. No names or e-mail addresses will be saved, and the information you provide will not be used for any other purpose. We plan to include the statistics in a poster to be presented at the ASMS conference this year. If you would like the results, we will be glad to send them to you. Thank you for your help. David Bostwick ****************************************************************************** From: moljaca@ccvd.com (Miodrag Oljaca) Date: Fri, 22 Jan 1999 19:38:14 GMT Subject: spectral de-convolution Organization: MindSpring Enterprises I am trying to deconvolve spectra measured using optical emission spectroscopy. Is anyone familiar with the software capable of deconvolving spectra into individual (Gaussian shape?) curves? The spectra I am measuring seems to be a convolution of 3 or 4 curves at different wavelengths. Miodrag Oljaca ****************************************************************************** From: "John G. Nikelly" Date: Sat, 23 Jan 1999 11:53:15 -0500 Subject: Trio2 and trio 2000 users Organization: * To all, We use a Trio2000 for ES work. We would like to share our experiences and benefit by the experiences of others who have used this instrument. If anyone currently or previously has used this instrument please contact me at . Our experience is that keepeing these instruments running and producing quality data is time consuming and expensive. If enough people are interested we can set up a support group for the exchange of ideas and spare parts. Darryl Davis Dept. of Chemistry University of the Sciences in Philadelphia John Nikelly John G. Nikelly Chemistry Department University of the Sciences in Philadelphia 600 South 43rd Street Philadelphia Pa 19104 Phone: (215) 596 8848 Fax: (215) 895 1100 j.nikell@usip.edu ****************************************************************************** From: David Bostwick Date: Sun, Jan. 24, 1999 Subject: ADMIN: Disk problems Organization: Georgia Institute of Technology The disk drive for on the incoming mail server for the newsgroup had a head crash on Saturday. Incoming mail is currently disabled, and the messages that were on the drive at that time may be lost. From the announcements that have been posted, we are not sure if incoming mail will bounce, if it will be saved in a queue, or if it will be discarded. We will let you know when the system is fully operational again. If you sent in a post, but do not see it after the drive is repaired, please submit it again. We apologize for the inconvenience. The contact address, , is on a different machine and is still working. You may use that address if you have questions. David Bostwick ****************************************************************************** From: david.bostwick@chemistry.gatech.edu (David Bostwick) Date: Mon, 25 Jan 99 15:08:12 GMT Subject: ADMIN: Disk problems repaired Organization: Georgia Institute of Technology, Atlanta GA, USA The drive that crashed has been repaired. The drive crashed about midnight Saturday (EST), two hours after the latest backup, so we don't believe any postings were lost. If you sent in an article, however, and don't see it within a day or so, please resubmit it. Thank you again for your patience. Also, thanks to those who have already responded with the information we requested earlier. David Bostwick ****************************************************************************** From: VRex Info Date: Mon, 25 Jan 1999 17:45:35 +0000 Subject: ANNOUNCEMENT: Visualizing data in stereoscopic 3D Organization: VRex, Inc. There is a new way to visualize detailed models in stereoscopic 3D on SGI workstations using AVS. AVS is an integrated and flexible visualization and analysis product for medical, engineering, manufacturing, and scientific visualization. It is a powerful package with capabilities for analyzing and simulating complex models and for communicating information about model data structures. VRex, Inc. has a new plug-and-play 3D stereoscopic projection system, the VR-2210 that works very well with SGI workstations running AVS. The projector makes it possible for groups of people to view AVS output easily and comfortably in flicker- free stereoscopic 3D. Displaying images in stereoscopic 3D makes it easier to see details that are lost in ordinary 2D displays. Stereoscopic 3D enhances "3D rendering" by adding depth information to the perspective view. The result is an image that not only has 3D perspective but that also appears to float in space inside and in front of the screen. Stereoscopic 3D makes crystal clear which data structures are in front and which are behind in any complex model. The projector is portable and uses passive polarized glasses. It works well for audiences of up to 200 people and can be used in classrooms, labs, and conference settings or for any other type of technical presentation. For users of AVS, on SGI platforms, the procedure to enable stereo viewing is simple. VRex's website is http://www.vrex.com and the phone number is (914) 345-8877. An application note that explains how to use the VR-2210 with AVS5 for the SGI is available at http://www.vrex.com/apps/html/avs5.html. Specific informaiton on the projector is at http://www.vrex.com/html/vr-2210.htm. Also visit http://www.vrex.com/apps/ for other supported applications. If you use a visualaztion application other than AVS that supports stereo, please let us know. We will be glad to help you determine if your application works with our projection system. ****************************************************************************** From: Thierry Date: Tue, 26 Jan 1999 13:25:25 +0100 Subject: Re: spectral de-convolution Organization: Universites Paris VI/Paris VII - France We have developped a MS data processing software based on the Maximum Entropy algoritm, used in different fields (ie radioastronomy, electron spectroscopy, IR, NMR and MS). Here are some communications: - J.Skilling and R.K. Bryan, Mon. Not. R. astr. Soc., 1984, 211, 111-124 - B.B. Reinhold and V.N. Reinhold, J. Am. Soc. Mass Spect., 1992, 3, 207-215 and a lot more... Hoping this helps, Miodrag Oljaca a écrit: } } I am trying to deconvolve spectra measured using optical emission } spectroscopy. Is anyone familiar with the software capable of } deconvolving spectra into individual (Gaussian shape?) curves? The } spectra I am measuring seems to be a convolution of 3 or 4 curves at } different wavelengths. } Miodrag Oljaca Thierry Blasco UFR Chimie Universite Paris VI France ****************************************************************************** From: David Fritze Date: Tue, 26 Jan 1999 08:36:31 -0600 Subject: Balzers Turbo Pump/Controllers Organization: Horizon Instrument Group FOR SALE: Balzers/Pfeiffer TPH-330 molecular turbo pump Balzers/Pfeiffer TCP-380 turbo pump controller VERY GOOD CONDITION! Both for $2500 or best offer Also for sale (best offer): Balzers/Pfeiffer TCP-300 turbo pump controller Balzers/Pfeiffer TCP-040 turbo pump controller If interested, please contact: David Fritze Horizon Instrument Group LBTC, South Stadium Rd. Baton Rouge, LA 70803-6100 USA Phone: (225) 388-5075 FAX: (225) 388-3975 E-Mail: info@horizon2.com ****************************************************************************** From: Dan Heglund Date: Wed, 27 Jan 1999 08:47:42 -0700 Subject: Class on spectral identification Organization: South Dakota School of Mines and Technology All: Just to let you know that I'm teaching a class this semester at the South Dakota School of Mines and Technology on spectral identification and some of my students may be stopping by and talking to some of you about the class. Most of the students are not familiar with newsgroups and this should be a fun experience for all involved. For those who helped me find a good used GC/MS about a year ago, thanks. Dan Heglund SD School of Mines Dept of Chem/ChemE 501 E. St. Joseph St. Rapid City, SD 57702 ****************************************************************************** From: david.bostwick@chemistry.gatech.edu (David Bostwick) Date: Wed, 27 Jan 99 18:01:53 GMT Subject: ADMIN: Mail server planned downtime Organization: Georgia Institute of Technology, Atlanta GA, USA The incoming mail server for the newsgroup will be down tonight from 6 p.m. to midnight (EST, GMT -5) to finish repairs that caused the head crash a few days ago. Messages sent to the newsgroup should be held in the queue and delivered when the server is back up. David Bostwick ****************************************************************************** From: Ron Orlando Date: Wed, 27 Jan 1999 15:19:59 -0500 Subject: Atlanta/Athens Discussion Group Meeting Organization: * January 27, 1999 To: AAMSDG Members From: Ron Orlando Our next meeting will be held in the auditorium of the Complex Carbohydrate Research Center (CCRC) on Thursday, February 4 at 7:00 PM. Directions to the CCRC follow. The primary speaker for the evening is Professor Craig Lunte (Department of Chemistry, University of Kansas) who will give us a presentation entitled "Capillary Electrophoresis of Biological Samples: Issues in Injection and Detection". So, mark your calendars -- I am sure that you will want to attend. I would also like to ask for "less senior" volunteer to give the short presentation at this meeting. Please let me know if you have any questions regarding the upcoming meeting. We hope everyone will be able to attend. Ron Corporate Sponsor of AAMSDG: Bruker Instruments, Inc. Robert Adams 44 Manning Rd. Billerica, MA 01821 Telephone: (404) 255-4817 FAX: (404) 847-9892 E-mail: raadams@IX.NETCOM.COM PE Applied Biosystems David Griffin 850 Lincoln Centre Drive Foster City, CA 94404 Telephone: (800) 545-7547 Fax: (650) 572-2743 E-mail: GriffiDM@perkin-elmer.com Micromass, Inc. Tom Beaty 713 Brookfield Road Raleigh, NC 27615 Telephone: (919) 676-8467 E-mail: Tom_Beaty@MSPEOPLE.COM JEOL USA, Inc. Felix Salinas 11 Dearborn Rd. Peabody, MA 01961-6043 Telephone: (281) 996-7787 FAX: (281) 996-7787 E-mail: salinas@JEOL.COM ThermoQuest Corporation Randall L. Porch 450 Franklin Road, Suite 130 Marietta, GA 30067 Telephone: (770) 424-7880, (770) 671-8146 FAX: (770) 423-1114 E-mail: 103455.2414@compuserve.com Kratos Analytical, Inc. Brian K. Stall 100 Red Schoolhouse Rd. Building A Chestnut Ridge, NY 10977 Telephone: (914) 426-6700 FAX: (914) 426-6192 E-mail: bkstall@aol.com ------------------- Directions to the Complex Carbohydrate Research Center from Atlanta 1. Follow I-85 North to Highway 316 (Exit to Lawrenceville/Athens). 2. Follow Highway 316 until it merges with the Epps Bridge Road/Georgia Loop 10 exchange. You will take a right onto the entrance of Georgia Loop 10 (East - this entrance to Georgia Loop 10 is before you cross the bridge that goes over the loop). 3. Follow Georgia Loop 10 to the College Station Road exit. Take this exit. 4. Take a right onto College Station Road. 5. At the first traffic light, take a right onto Riverbend Road. 6. Take a direct right into the parking lot. CCRC will be the second building on your right (the first building is the Riverbend Research Laboratories). -------------------------- ___________________________________________________________________ Ron Orlando Complex Carbohydrate Research Center and Department of Biochem. and Mol. Bio., University of Georgia E-Mail: Orlando@ccrc.uga.edu Phone: (706) 542-4429 ___________________________________________________________________ ****************************************************************************** From: "Dr. Efraim Graber" Date: Wed, 27 Jan 1999 23:12:27 +0200 Subject: MS-data of food flavors aroma chemicals Organization: * Hello, I am seeking to find MS data (spectra) for aroma chemicals which are used in the production of food flavors. This data could be on the net or in books. Thank you in advance, Dr. Efraim Graber Florasynth Israeli Enterprise LTD ****************************************************************************** From: "Marty Teltscher" Date: Wed, 27 Jan 1999 21:24:20 -0500 Subject: MALDI sample prep.: High MW Membrane proteins Organization: McGill University Computing Centre I'm researching the protein FhuA in the lab of Dr. J.W. Coulton. The protein I'm using contains a histidine tag and has a molecular weight of 79852 Da. I'm new to MALDI and have been trying to characterize the protein by mass analysis for months. Aside from its large size, FhuA is a membrane protein, found in the outer membrane of E. coli K-12. This adds the requirement for detergents. The detergent buffering the protein is currently LDAO, but we have also used octyl glucoside. Any help or recommendations as to how I should prepare the sample, configure the MALDI-TOF (Perceptive Biosystems Voyager DE), or direction to literature that might help me would be greatly appreciated. recent crystal structure of FhuA in Science: http://www.sciencemag.org/cgi/content/full/282/5397/2215 Marty Teltscher email: mtelts@po-box.mcgill.ca @mcgill university department of microbiology and immunology montreal.quebec.canada ****************************************************************************** From: Young Min Date: Thu, 28 Jan 1999 17:01:05 +0900 Subject: help me for spec 'VG 95034' Organization: Inet Internet Services Dear Sir. Help me please! I don't know and understanding that What kind of standard for 'VG 95034' or 'Benutzungsrecht nach VG 95034' Anyone, teaching me. please! Thank you ****************************************************************************** From: Karel Lazou Date: Thu, 28 Jan 1999 15:24:49 +0100 (MET) Subject: LC/MS buffer problems Organization: * Dear, I am doing LC-MS using Electrospray and APCI. I did some 'succesfull' analysis using an unvolatile phosphate buffer (with a serious sensitivity loss +/- 50 %of course, but with a signal!!!!). Now, when I do one very short analysis with orthophosphoric acid in the flow, I immediately got problems with the 'spray chamber electronics' and I even have to clean the cappilary (connection to the vacuum part) (no signal!!!). What is happening with the salt(even more friendly NH4Ac), which is dissociated in solution in the LC flow, in the evaporating droplets? (recombination and precipitation in the atmospheric pressure region as the solubility limit is reached?) How can the ES interface being seen as an 'electrolitic cell' in this respect. And what is the difference than for the orthophosphoric acid and the cause of this problems comparing with the PO4--- ions from the buffer? Thank you in advance for any comment Karel Lazou org. chem. /RUG Krijgslaan 281 S4 9000 Gent; Belgium Karel.Lazou@RUG.ac.be ****************************************************************************** From: "Tata, Prasad" Date: Thu, 28 Jan 1999 18:29:59 -0500 Subject: Total Flavanoid content in Grapefruit juice Organization: * Dear Members: Would someone provide me any reference on Measurement of total flavanoids in Grapefruit juice or any fruit juice. I am specifically looking for measuring Falvanoids in an natural source. Thanks in advance, Sincerely, Prasad NV Tata, Ph.D. Otsuka America Pharmaceutical, Inc. 2440 Research Blvd. Rockville, MD 20850 ****************************************************************************** From: ricksfbrsc@aol.com (Ricksfbrsc) Date: 29 Jan 1999 01:06:15 GMT Subject: Re: MS-data of food flavors aroma chemicals Organization: AOL http://www.aol.com Walter Jennings, et al, wrote a book with several hundred spectra. It's somewhat old, but a place to start. These spectra are also in the Wiley or NBS databases, I believe. ****************************************************************************** From: "Peter" Date: Fri, 29 Jan 1999 12:12:14 +0100 Subject: Accurate mass vs. Peak-matching Organization: MSOCKUN Hello mass spec people, I was wondering who could explain the difference between the following: - accurate mass measurenment - peak matching - exact mass measurement do they all three mean the same and serve the same purpose? What tolerances are acceptable?? thanks for any reaction to this topic. Peter ****************************************************************************** From: "Fred Strobel" Date: Fri, 29 Jan 1999 08:38:27 -0500 Subject: Re: Accurate mass vs. Peak-matching Organization: * -----Original Message----- From: Peter Newsgroups: sci.techniques.mass-spec Date: Friday, January 29, 1999 7:26 AM Subject: Accurate mass vs. Peak-matching }I was wondering who could explain the difference between the }following: } }- accurate mass measurenment }- peak matching }- exact mass measurement } }do they all three mean the same and serve the same purpose? accurate mass measurement and exact mass measurement do mean the same. Peak matching is an experimental mental method to measure the masses. }What tolerances are acceptable?? Traditionally, it has been 5 ppm (parts per million) has been the standard. This has been used on sector instruments for a long period. However, it has been suggested that a statistical analysis of the method used in the measurement be done and the results be in the 95% confidence level. This will mean that different experiments will have different mass accuracy tolerances, for example, MALDI using time-of-flight have been measuring mass accuracies on the order of 10 ppm, this would be acceptable. The most important thing is that the accuracy be good enough to distinguish the REAL possibilities for the compound. An example, is the use of exact masses in synthesis. Exact mass is required for publication, but how many synthetic pathways could subsitute a N atom for CH2 or an O for a CH4, nominal masses would probably be fine for at least 90% of the measurements made because of the limited pathways in chemical reactions. Fred Strobel Director of Emory University Mass Spectrometry Center ****************************************************************************** From: "Ioannis Papayannopoulos" Date: Sat, 30 Jan 1999 22:36:43 -0500 Subject: Re: Accurate mass vs. Peak-matching Organization: ISPNews http://ispnews.com Accurate mass measurement and exact mass measurement are being used interchangeably, although, in my opinion, the former is measured whereas the latter is calculated. Thus the (measured) accurate mass is compared to the (calculated) exact mass. Accuarate mass is measured within an error window, defined by the instrument and overall experimental conditions. For small molecules this can be used to obtain a limited number of possible element combinations that add up to the measured accurate mass, so elemental compositions can be determined in this fashion. Peak matching is a technique used with magnetic deflection sector instruments operating at high resolution mode (typically m/Dm >= 10000), whereby a particular monoisotopic peak of an unknown is superimposed to a peak of an internal standard (the exact mass of which can be calculated); the two molecules, unknown and internal standard, must be different but close in mass. This was (I am using the past tense since I doubt many people do this sort of thing anymore) done by introducing a magnetic field in addition to that supplied of the instrument magnet. By changing the magnitude of the additional magnetic field, and alternating between switching it on and off, the two peaks, unknown and internal standard could be made to appear on the same position on the instrument's oscilloscope, thus made to overlap, hence "peak matching". -- Ioannis Papayannopoulos Astra Arcus USA, Inc. Worcester, MA Peter wrote in message <78s9dq$r3a@acmex.gatech.edu>... }Hello mass spec people, } }I was wondering who could explain the difference between the }following: } }- accurate mass measurenment }- peak matching }- exact mass measurement } }do they all three mean the same and serve the same purpose? } }What tolerances are acceptable?? } } }thanks for any reaction to this topic. } }Peter } } } } ****************************************************************************** From: clee751159@aol.com (CLee751159) Date: 31 Jan 1999 10:04:44 GMT Subject: Re: Accurate mass vs. Peak-matching Organization: AOL, http://www.aol.fr }Accurate mass measurement and exact mass measurement are being used }interchangeably, although, in my opinion, the former is measured whereas }the latter is calculated. Thus the (measured) accurate mass is compared }to the (calculated) exact mass. Accuarate mass is measured within an }error window, defined by the instrument and overall experimental }conditions. For small molecules this can be used to obtain a limited }number of possible element combinations that add up to the measured }accurate mass, so elemental compositions can be determined in this }fashion. } }Peak matching is a technique used with magnetic deflection sector }instruments operating at high resolution mode (typically m/Dm >= 10000), }whereby a particular monoisotopic peak of an unknown is superimposed to a }peak of an internal standard (the exact mass of which can be calculated); }the two molecules, unknown and internal standard, must be different but }close in mass. This was (I am using the past tense since I doubt many }people do this sort of thing anymore) done by introducing a magnetic field }in addition to that supplied of the instrument magnet. By changing the }magnitude of the additional magnetic field, and alternating between }switching it on and off, the two peaks, unknown and internal standard }could be made to appear on the same position on the instrument's }oscilloscope, thus made to overlap, hence "peak matching". -- Ioannis }Papayannopoulos Astra Arcus USA, Inc. Worcester, MA } }Peter wrote in message <78s9dq$r3a@acmex.gatech.edu>... }}Hello mass spec people, }} }}I was wondering who could explain the difference between the }}following: }} }}- accurate mass measurenment }}- peak matching }}- exact mass measurement }} }}do they all three mean the same and serve the same purpose? }} }}What tolerances are acceptable?? }} }} }}thanks for any reaction to this topic. }} }}Peter }} Just 2 small corrections: 1) Peak matching was done, at least with Micromass machines by switching the accelerating voltage; m/z is proportional to 1/V. You couldn't set the magnetic field accurately enough for this purpose. A sawtooth waveform was imposed on the voltage in order to scan the peaks. Perhaps, in other machines the scan was obtained by wiggling the magnetic field. Nowadays, if your ionisation technique allows the use of a mass deficient reference compound like PFK, you just scan the spectrum and leave the computer to do the work. 2) In the 1970's, Hammar and others showed that theoretically you don't need high resolution if your mass peak is homogeneous, as is often the case. As a rough guide, you can estimate the centroid of a not-too-noisy peak to about 1% of its width. Present TOF machines give about 5000 resolution. This is usually good enough (for medicinal chemistry etc.), but mass measurement is more difficult with API (LC/MS) than with EI, because we don't have mass deficient reference compounds. Minor peaks in the PEG spectra tend to interfere mass-wise with the general run of compounds being analysed. Regards ****************************************************************************** From: cciti@hotmail.com Date: Sun, 31 Jan 1999 15:24:33 GMT Subject: CE-ESI-MS Organization: Deja News - The Leader in Internet Discussion Hi, all, Does anybody have such experience on the cereal protein analysis, please notify me about that. -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Accurate mass vs. Peak-matching Date: Mon, 1 Feb 1999 16:17:13 GMT Organization: JEOL USA, Inc. In follow up to questions about the definitions of "exact mass", "accurate mass", and "peak matching": In <790jnm$5ip@acmex.gatech.edu> "Ioannis Papayannopoulos" writes: }Accurate mass measurement and exact mass measurement are being used }interchangeably, although, in my opinion, the former is measured whereas }the latter is calculated. Thus the (measured) accurate mass is compared }to the (calculated) exact mass. I have used the terms in the same way. However, the recent recommendations on terminology favor the term "exact mass measurement" over "accurate mass measurement" to describe the analytical procedure. The explanation has to do with the fact that for low-resolution measurements, an integer-mass result could be considered "accurate" in the sense that this is the answer that you are looking for. }Peak matching is a technique used with magnetic deflection sector }instruments operating at high resolution mode (typically m/Dm >= 10000), }whereby a particular monoisotopic peak of an unknown is superimposed to a }peak of an internal standard (the exact mass of which can be calculated); }the two molecules, unknown and internal standard, must be different but }close in mass. This was (I am using the past tense since I doubt many }people do this sort of thing anymore) done by introducing a magnetic field }in addition to that supplied of the instrument magnet. By changing the }magnitude of the additional magnetic field, and alternating between }switching it on and off, the two peaks, unknown and internal standard }could be made to appear on the same position on the instrument's }oscilloscope, thus made to overlap, hence "peak matching". -- Ioannis Peak matching is less common these days, but it is still done. The magnet position is fixed, and the accelerating voltage is switched between a known and reference peak. The accelerating voltage difference can be used to determine the mass difference between the unknown peak and the standard peak. Of course, you must calibrate the peak matching by using two reference points to define the slope and intercept. This is done prior to making a series of measurements. A second magnet coil is sometimes used, but its purpose is just to sweep the peak over a narrow range so that you can see the peak shape on the oscilloscope. The mass measurement comes from the accelerating voltage (and electric sector) switching. Peak matching can be done manually (with a precision voltage divider) or under computer control. Voltage sweeps or magnet scanning can also be used to measure exact masses. I've had good luck on small sectors by using a lock mass. Concerning the questions about what level of accuracy is needed, the exact mass measurement is really best thought of as another piece of information to be used together with isotope ratio measurements, sample history, and so on. The information is supporting evidence for an expected composition, or it is a valuable clue to the identity of an unknown. The 5 ppm limit is arbitrary. In fact, my personal opinion is that 'ppm' is a poor choice for exact mass measurements because a part-per-million definition places tighter restrictions on small masses (where there are few elemental combinations that are possible matches) than for larger masses, where you need all of the accuracy that you can get to distinguish among a large number of possibilities. A definition in terms of millimass units is probably more reasonable. It can be argued that exact mass measurements are of limited value for larger molecules. I've solved some compositions for unknowns with mass-to-charge ratios above 1000u, but only by taking into account other information about the sample together with the exact mass. ------------ >Peter wrote in message <78s9dq$r3a@acmex.gatech.edu>... }}Hello mass spec people, >} >}I was wondering who could explain the difference between the >}following: >} >}- accurate mass measurenment }}- peak matching >}- exact mass measurement >} >}do they all three mean the same and serve the same purpose? >} >}What tolerances are acceptable?? >} >} >}thanks for any reaction to this topic. >} }}Peter >} >} >} >} -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: mauber@to.flashGORDONnet.it (mauro) Date: Mon, 01 Feb 1999 22:24:35 GMT Subject: API 365 vs TSQ7000 Organization: No one Dear all, In my chromatographic laboratory at the Pharmacokinetics Unit we are looking for a new LC-MS. Wwe were offered an API365 at a special price since it was a demo instrument, obviously guaranteed by PE; aAt the same time we were offered to substitute our Finningan LCQ with their triple quadrupole TSQ 7000. Before choosing which one to buy, I would like to know the opinion of some user about these 2 instruments, taking into accound that the main use will be analysis of plasma samples coming from clinical studies. Every information will be greatly appreciated. -- Mauro Bertolino Per rispondere rimuovere GORDON dall'indirizzo e-mail To reply remove GORDON from e-mail address ****************************************************************************** From: wd1v@mediaone.net (John Seney) Date: Mon, 01 Feb 1999 19:13:28 -0500 Subject: Scope FAQ Organization: WD1V .-. / \ / \ / \ / \ .-. .-. .- \ / \ / \ / \ / \ / \ / `-' `-' `-' Oscilloscope FAQ: http://people.ne.mediaone.net/wd1v/dsofaq.html ****************************************************************************** From: Ron Orlando Date: Tue, 2 Feb 1999 18:14:24 -0500 Subject: Atlanta Athens Mass Spectrometry Discussion Group Announcement Organization: * To: AAMSDG Members From: Ron Orlando This is a reminder that our next meeting will be held in the auditorium of the Complex Carbohydrate Research Center (CCRC) on Thursday, February 4 at 7:00 PM. Directions to the CCRC follow. The primary speaker for the evening is Professor Craig Lunte (Department of Chemistry, University of Kansas) who will give us a presentation entitled "Capillary Electrophoresis of Biological Samples: Issues in Injection and Detection". I would also like to ask for "less senior" volunteer to give the short presentation at this meeting. Please let me know if you have any questions regarding the upcoming meeting. We hope everyone will be able to attend. Ron Corporate Sponsor of AAMSDG: Bruker Instruments, Inc. Robert Adams 44 Manning Rd. Billerica, MA 01821 Telephone: (404) 255-4817 FAX: (404) 847-9892 E-mail: raadams@IX.NETCOM.COM PE Applied Biosystems David Griffin 850 Lincoln Centre Drive Foster City, CA 94404 Telephone: (800) 545-7547 Fax: (650) 572-2743 E-mail: GriffiDM@perkin-elmer.com Micromass, Inc. Tom Beaty 713 Brookfield Road Raleigh, NC 27615 Telephone: (919) 676-8467 E-mail: Tom_Beaty@MSPEOPLE.COM JEOL USA, Inc. Felix Salinas 11 Dearborn Rd. Peabody, MA 01961-6043 Telephone: (281) 996-7787 FAX: (281) 996-7787 E-mail: salinas@JEOL.COM ThermoQuest Corporation Randall L. Porch 450 Franklin Road, Suite 130 Marietta, GA 30067 Telephone: (770) 424-7880, (770) 671-8146 FAX: (770) 423-1114 E-mail: 103455.2414@compuserve.com Kratos Analytical, Inc. Brian K. Stall 100 Red Schoolhouse Rd. Building A Chestnut Ridge, NY 10977 Telephone: (914) 426-6700 FAX: (914) 426-6192 E-mail: bkstall@aol.com ------------------- Directions to the Complex Carbohydrate Research Center from Atlanta 1. Follow I-85 North to Highway 316 (Exit to Lawrenceville/Athens). 2. Follow Highway 316 until it merges with the Epps Bridge Road/Georgia Loop 10 exchange. You will take a right onto the entrance of Georgia Loop 10 (East - this entrance to Georgia Loop 10 is before you cross the bridge that goes over the loop). 3. Follow Georgia Loop 10 to the College Station Road exit. Take this exit. 4. Take a right onto College Station Road. 5. At the first traffic light, take a right onto Riverbend Road. 6. Take a direct right into the parking lot. CCRC will be the second building on your right (the first building is the Riverbend Research Laboratories). -------------------------- -- ___________________________________________________________________ Ron Orlando Complex Carbohydrate Research Center and Department of Biochem. and Mol. Bio., University of Georgia E-Mail: Orlando@ccrc.uga.edu Phone: (706) 542-4429 ___________________________________________________________________ ****************************************************************************** From: Dan Garteiz Subject: A Y2K Solution for HP Engines Date: Wed, 3 Feb 1999 08:08:18 -0600 Organization: * [This is from sci.chem.analytical. DB] According to HP, in order to get my engine to comply with Y2K i need to purchase a new data system, software, and set up some kind of conversion for the data files. Needless to say, that sounds a bit unnecessary. I developed a solution that does not require a new data system or new data analysis software. See it at: http://www.ghg.net/texms/hpy2k/hpy2k.html ****************************************************************************** From: sumner@mail.chem.tamu.edu (Lloyd Sumner ) Date: Wed, 03 Feb 1999 09:56:03 -0600 Subject: Re: Total Flavanoid content in Grapefruit juice Organization: * Prasad, Mass spectrometry of flavonoids goes back a significant way in time (~20yrs). Following are a few references to get you started. Our paper has a good list of references covering MS of flavonoids prior to our 1996 publication date. The two newer publications listed should be more informative on recent developments. You might also search the Proceedings of the annual ASMS meetings. I recall seeing several presentations over the years specificly describing flavonoid profiling as a tool used in the identification of adulterated fruit juices. Sumner, Paiva, Dixon, Geno, J. Mass Spectrom. 1996, 31, 472-485. Watson and Pitt, Rapid Commun. Mass Spectrom. 1998, 12, 153-156 Barnes, Smith, Williams, Damant, Shepherd, Rapid Commun. Mass Spectrom. 1998, 12, 130-138. Best Regards, Lloyd Sumner Prasad NV Tada wrote: } }Dear Members: } }Would someone provide me any reference on Measurement of total flavanoids }in }Grapefruit juice or any fruit juice. I am specifically looking for }measuring }Falvanoids in an natural source. } }Thanks in advance, } }Sincerely, } }Prasad NV Tata, Ph.D. }Otsuka America Pharmaceutical, Inc. }2440 Research Blvd. }Rockville, MD 20850 Lloyd W. Sumner, Ph.D. Associate Director, The Laboratory for Biological Mass Spectrometry Texas A&M University Department of Chemistry P.O. Box 30012 College Station, TX 77842-3012 Voice #409-845-8404 Fax #409-845-4719 Email Sumner@mail.chem.tamu.edu For more information see our webpage at http://www.chem.tamu.edu/services/ms/ For more information on The Center for Chemical Characterization and Analysis see: http://www.chem.tamu.edu/CCCA/ ****************************************************************************** From: Jagan Annamalai Date: Wed, 03 Feb 1999 12:49:47 -0600 Subject: Newbie questions on Mass Spec Data Interpretation Organization: University of Houston Hi ! The Dycor Quadropole analyzer manual has a table titled 'Pre-programmed Spectral Library'. For oxygen, it lists 32/100 16/11. My interpretation was that when pure oxygen is sent to the system, the partial pressures at 32 (O2) and 16 (O) will be in the ratio 100:11. This is what the tech support also said. The same table lists for carbon dioxide : 44/100 (CO2) 28/11 (CO) 16/9 (O) 12/6 (C) 45/1 (CO2) 22/1 (??) What is the species with amu = 22? The feed is pure CO2. Any O, CO, C should come from CO2. Shouldn't the value at 16 be more than 9. Doing a mole balance: value at 16 = value at 28 + 2*value at 12 = 11 + 2*6 = 23 Shouldn't this be the value? Any comments/suggestions on this is appreciated. Thanks, -Jagan ****************************************************************************** From: John Date: Wed, 03 Feb 1999 15:03:41 -0500 Subject: Newbie Question ICP-MS Organization: Posted via RemarQ, http://www.remarQ.com - Discussions start here! Can someone please describe the theory of an ICP-MS? What is is used for? How does it work? etc.... Don't get too scientific on me, I am just starting out. Thank you in advance. PS: any good web sites on theory and use of scientific equipment??? johnm ****************************************************************************** From: Peter Nastold Date: Thu, 04 Feb 1999 10:45:11 +0100 Subject: Re: Newbie questions on Mass Spec Data Interpretation Organization: * Jagan Annamalai schrieb: } Hi ! } } The Dycor Quadropole analyzer manual has a table titled } 'Pre-programmed } Spectral Library'. For oxygen, it lists 32/100 16/11. My } interpretation was that when pure oxygen is sent to the system, the } partial pressures at 32 (O2) and 16 (O) will be in the ratio 100:11. } This is what the tech support also said. } } The same table lists for carbon dioxide : } } 44/100 (CO2) 28/11 (CO) 16/9 (O) 12/6 (C) 45/1 (CO2) 22/1 } (??) } } What is the species with amu = 22? Double charged CO2. Be careful: you don't get your result in amu but in the mass-to-charge ratio! Normally the charge is 1 but higher charged species can occur in minor quantities. } } } The feed is pure CO2. Any O, CO, C should come from CO2. Shouldn't } the } value at 16 be more than 9. } } Doing a mole balance: } } value at 16 = value at 28 + 2*value at 12 = 11 + 2*6 = 23 } } Shouldn't this be the value? } } Any comments/suggestions on this is appreciated. } } Thanks, } } -Jagan In your detector you'll only detect ions - very simple. But in the fragmentation of an ion you'll get only one radical ion while the other particle is an uncharged radical i.e. undetectable. Now suppose that the cleavage of a special bond won't give a 50% chance to each of the resulting fragments to get the charge. So one fragment will give a stronger signal than the other although they both are formed in a 1:1 ratio. In a simple molecule like O2 this doesn't matter because you either get O2 or O; the same for other symmetric fragmentations. BTW I don't agree to the partial pressure thing. All you can say is that the ratio of O2-ions and O-ions caught by the detector is 100:11. This doesn't explain the quantity of the gap you realized but at least its quality. Peter ****************************************************************************** From: Yining Zhao Subject: Ion-pair reagents in LC-MS Date: Thu, 4 Feb 1999 11:19:30 +0100 (MET) Organization: * Hi, Gentlemen: I have one application on LC-API-ESMS using ion-pair HPLC principle, as you know, the ion-pair reagents are normally unfriendly to MS. However, I found someone were using triethylamine heptafluorobutyric acid (HFBA) or tetraethyl/tetrabutylammonium hydroxide (TEAH, TBAH)in the LC-MS analysis in the literature, they didn't mention any contamination or ion-evaporation probelms. I'd like to have your opinion on those ion-pair reagents, are they really suitable for LC-MS, if yes, what's the intrinsic reasons ? Thanks a lot ---------------------------------- ****************************************************************************** From: John Barton Date: Thu, 04 Feb 1999 10:22:48 +0000 Subject: Re: Newbie questions on Mass Spec Data Interpretation Organization: Imperial College, London, UK Jagan Annamalai wrote: } } Hi ! } } The Dycor Quadropole analyzer manual has a table titled 'Pre-programmed } Spectral Library'. For oxygen, it lists 32/100 16/11. My } interpretation was that when pure oxygen is sent to the system, the } partial pressures at 32 (O2) and 16 (O) will be in the ratio 100:11. } This is what the tech support also said. } } The same table lists for carbon dioxide : } } 44/100 (CO2) 28/11 (CO) 16/9 (O) 12/6 (C) 45/1 (CO2) 22/1 (??) } } What is the species with amu = 22? } } The feed is pure CO2. Any O, CO, C should come from CO2. Shouldn't the } value at 16 be more than 9. } } Doing a mole balance: } } value at 16 = value at 28 + 2*value at 12 = 11 + 2*6 = 23 } } Shouldn't this be the value? } } Any comments/suggestions on this is appreciated. } } Thanks, } } -Jagan The peak at 22 is doubly-charged CO2 [CO2]++. A mass spectrometer measures mass/charge ratio (m/z) not mass. In most cases (with the exception of electrospray ionisation) the charge state is 1, so m/z and m are numerically equivalent. It is possible, however, to remove more than one electron from a molecule (or element), giving a value of z of, in this case, 2. The mass spectrometer will therefore record the ion as 44/2=22. Whether a molecule can be doubly charged or not depends in part on the SECOND ionisation energy of the species. For most molecules this is too high for significant M++ production, but some molecules (particularly aromatics), exhibit significant M++ peaks in their spectrum. Naphthalene is a good example. The intensity of fragment peaks in a mass spectrum is not related to the "mole balance" of the elements. Peak intensities in a mass spectrum are not readily calculable ****************************************************************************** From: DIETER.ZIMMER.DZ@bayer-ag.de Date: Thu, 4 Feb 1999 17:19:26 +0100 Subject: Re: API 365 vs TSQ7000 Organization: * }Mauro Bertolino wrote: }Dear all, } }In my chromatographic laboratory at the Pharmacokinetics Unit we are }looking for a new LC-MS. }Wwe were offered an API365 at a special price since it was a demo }instrument, obviously guaranteed by PE; aAt the same time we were }offered to substitute our Finningan LCQ with their triple quadrupole }TSQ 7000. }Before choosing which one to buy, I would like to know the opinion of }some user about these 2 instruments, taking into accound that the main }use will be analysis of plasma samples coming from clinical studies. } }Every information will be greatly appreciated. } }-- } }Mauro Bertolino I am working in Preclinical Pharmacokinetics. We are using PE Sciex Triple Quadrupoles for Quantitation of drugs out of Plasma obtained in the frame of pharmacokinetics and toxicokinetics (GLP) studies since 1992. First we used the API 3Plus, later the API 300, 365 and recently the API 3000 products. All of them is very rugged! The API 300 and 365 line is lower in sensitivity when compared to the "old" API 3PLus "gold standard". However, the recently introduced API 3000 is definitely more sensitive than the API 365 and the API 3Plus. Finnigan sells (very recently) a new Ion source with their latest version of the TSQ 7000. I had no chance to run samples on the new ion source so far, therefore I have no data from the instrument and did not find applications in the literature yet.. However in the past the Sciex API Triple Quadrupoles proved more suitable for quantitation than any other instruments in the market. In the literature you will find more papers where a Sciex Triple Quad was used for quantitation than use of other instruments. I could imagine that the TSQ 7000 with the new ion source is more sensitive than the API 365 family, since sensitivity was never the problem of the TSQ 7000. But the heated capillary interface of the TSQ 7000 caused more need for maintenance in daily routine work than the simple and rugged design of the Sciex API ion source. Thus, my advice: run your samples on both type of instruments and make your decision! If you have a chance to buy an API 3000 this might be the best deal regarding sensitivity (and ruggedness) for quantitation out of plasma. (This is my personal opinion and does not reflect the opinion of my company!) Dieter Zimmer ****************************************************************************** From: Jagan Annamalai Date: Thu, 04 Feb 1999 13:43:50 -0600 Subject: Re: Newbie questions on Mass Spec Data Interpretation Organization: University of Houston Thanks for the replies! -Jagan ****************************************************************************** From: "M.S. Nieuwenhuizen" Subject: VG70 for sale Date: Thu, 04 Feb 1999 19:48:40 +0100 Organization: World Access [This is from sci.chem.labware. DB] WE have for sale a 10 years old VG70 GC-MS system. Please contact M. Nieuwenhuizen P.O. Box 45 2280 AA Rijswijk The Netherlands nieuwenh@pml.tno.nl ****************************************************************************** From: "Roland Burton" Date: 4 Feb 1999 19:35:07 GMT Subject: Re: Ion-pair reagents in LC-MS Organization: The University of British Columbia Re ion pairing reagents and LCMS If the reagent is volatile, perhaps it will be pumped out of the mass spectrometer eventually and will not deposit salts on surfaces which may change their electrical properties, or even block orifices. Any ion pair reagent will reduce the amount of ionized analyte present, so will reduce signal strength in electrospray-type ionization. Ion pair reagents are hard to get out of your column. Once you have used a column with ion pair reagents, might as well keep it for that purpose. We have used amine ion pair reagents to look at negatively charged nucleotides, and this is the main source of our experience with ion pairing. Roland Burton Mass Spectroscopist Faculty of Pharmaceutical Sciences University of British Columbia 2146 East Mall Vancouver, B.C. CANADA V6t 1Z3 Yining Zhao wrote in article <79c390$qbl@acmey.gatech.edu>... } } Hi, Gentlemen: } } I have one application on LC-API-ESMS using ion-pair HPLC principle, as } you know, the ion-pair reagents are normally unfriendly to MS. However, I } found someone were using triethylamine heptafluorobutyric acid (HFBA) or } tetraethyl/tetrabutylammonium hydroxide (TEAH, TBAH)in the LC-MS analysis } in the literature, they didn't mention any contamination or } ion-evaporation probelms. } I'd like to have your opinion on those ion-pair reagents, are they really } suitable for LC-MS, if yes, what's the intrinsic reasons ? Thanks a lot } ---------------------------------- } } } } ****************************************************************************** From: "Masahiko Okamoto Date: 5 Feb 1999 07:25:02 GMT Subject: Re: MALDI-TOF-MS Organization: * Galletti Guido wrote in article <77ngst$1bj@acmex.gatech.edu>... } In reply to Prof. Galletti, the updated list of MALDI-TOFMS appeared in Anal. Chem., November 1 1998, 733A-737A. I believe that this is an excellent summary of present status of MALDI-TOFMS. We have just bought a MALDI-TOFMS for the widest range of application like you. In our case, the resolution and the mass accuracy are extremely important to choose the appropriate machine, because we usually have got a pretty amount of sample(the sensitivity is not so critical). We scrutinized the performance of three mass spectrometers, RFLEX III (Bruker), Voyager DE-STR (PerSeptive), and Kompact SEQ (Shimadzu). Among them, the former two machines are equivalent in performance, but as to the PSD and automated sample loading, I think that RFLEX III is the best in these machines (very user-friendly). The price of various MALDI manufactures in US was also shown in this list. I felt strange, because it was much cheaper than that in Japan in general. I do not know why the MALDI-TOFMS in Japan is much more expensive than that in US. If anybody know the reason, please teach me ! Regard, Masahiko OKAMOTO, Ph.D Sumitomo Chemical Co., Ltd. Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd 3-1-98 Konohana-ku Osaka 554-0022 Japan E-mail:mokamoto@sumitomo-chem.co.jp FAX: +81-6-6466-5459 ****************************************************************************** From: "Kermit K. Murray" Date: Fri, 5 Feb 1999 13:16:30 -0500 Subject: Pat Limbach Seminar 2/9 at Emory Organization: * Pat will also be at UGA, Clemson, and Auburn later in the week. Emory University PMACS Seminar http://www.emory.edu/PMACS/ "What is the Chemystery of MALDI-MS?" Dr. Patrick Limbach Louisiana State University February 9, 1999 4:00 PM Room 100, Dental School 1462 Clifton Rd. The relatively recent development of matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOFMS) has improved greatly our ability to characterize large polar thermally labile compounds. While MALDI-TOFMS has proven to be a versatile analytical technique for the characterization of a large range of organic compounds, there are still significant problems associated with the analysis of several different classes of compounds. Future developments in the areas of structural biology, polymer chemistry, and materials science, among others, will require a viable method for the structural characterization of new and yet to be discovered compounds. This presentation will focus on our work aimed at understanding the chemical interactions between matrix and analyte, and using this understanding to develop new and improved experimental protocols for MALDI-MS. The discussion will highlight our work in the areas of oligonucleotide and polymer analysis. ****************************************************************************** From: Melania Date: Sat, 06 Feb 1999 03:57:41 GMT Subject: 5035 - Organization: Deja News - The Leader in Internet Discussion I am wondering if anyone has started running method 5035 regularly, and if so what instruments (autosampler) you are using, and how it is working. I am currently starting to get more requests for this and I am having trouble with the Internal Standards and Surrogates having very very low recoveries but only when actually purging a soil matrix the blanks and such work beautifully.. HELP.. I am currently using a Varian Archon autosampler with an HP 5890 GC/MS system, I know that the soil matrix can affect my recoveries but they are a lot worse than when running on a Tekmar 2016.. -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own ****************************************************************************** From: Thomas Storm Date: Sat, 06 Feb 1999 15:27:59 +0100 Subject: Re: Ion-pair reagents in LC-MS Organization: TU-Berlin Yinning Zhao wrote: }Hi, Gentlemen: }I have one application on LC-API-ESMS using ion-pair HPLC principle, as }you know, the ion-pair reagents are normally unfriendly to MS. However, I }found someone were using triethylamine heptafluorobutyric acid (HFBA) or }tetraethyl/tetrabutylammonium hydroxide (TEAH, TBAH)in the LC-MS analysis }in the literature, they didn't mention any contamination or }ion-evaporation probelms. I'd like to have your opinion on those ion-pair }reagents, are they really suitable for LC-MS, if yes, what's the }intrinsic reasons ? Thanks a lot ---------------------------------- Hello, while testing and and comparing some tertiary amines as ion-pairing agents (Triethylamine, Dimethylbutylamine and Triethylamine) for the the separation of aromatic sulfonates, we found a steady decrease of sensitivity with rising concentrations of ion-pairing agent. That means you have to find a compromise to get both reasonable retention and sensitivity. A good idea seems to be to remove the ion-pairing agent with an on-line desalting device (presented by Dr.T.Knepper, ESWE-Institute (www.uni-mainz.de/~eswe/) at the LC/MS Symposium in Montreux). You should be able to use any ion-pairing agent you like, and get rid of it before you introduce the sample into your MS. I personnally never worked with anionic ion-pairing agents. If you are using perfluorinated organic acids as ion-pairing agents, keep in mind that these compounds can be very sticky in the tubing of your HPLC, especially when using a vacuum degassing device (then semipermeable membranes are made of Teflon). Best Thomas Thomas Storm TU-Berlin Fachgebiet Wasserreinhaltung Sekr. KF4 Strasse des 17.Juni 135 10623 Berlin Tel. ++49 (0) 30 314 25480 FAX ++49 (0) 30 314 23850 e-mail: storm@itu203.ut.tu-berlin.de ****************************************************************************** From: "unknown" Date: Sat, 6 Feb 1999 19:55:10 -0500 Subject: Re: Accurate mass vs. Peak-matching Organization: ISPNews http://ispnews.com Thanks Chip Cody and CLee for the correction -- of course, it is the high voltage that is switched, as it can be controlled more accurately than the magnet current. When I responded I was picturing, in my mind, the extra coils in the old MAT731 which were used to "superimpose" the two peaks on the storage oscilloscope (no computer controls were available). Sorry for the inadvertent misinformation. Ioannis Papayannopoulos ****************************************************************************** From: Carl Braybrook Date: Mon, 8 Feb 1999 13:30:31 +1100 Subject: VG 70/70 FREE TO GOO