****************************************************************************** From: dond@mhv.net Date: Fri, 02 Jan 1998 22:11:41 GMT Subject: Re: ion trap vs. quadrapole Organization: MHVNet On 17 Dec 1997 08:38:13 -0500, "Ludwig Gruber" wrote: }} So far we have heard from the various manufactures contradicting }} information. Thus could anyone with experience on ion trap and quadrapole }} systems investigating complex and dirty systems please correspond }} detailing their experiences. } }There is a good compilation about this subject in Analytical Consumer, Vol 6 No. 5 from February 1996. } }In our experience (we own two ion traps and a numer of quadrupoles) the problem of classic ion traps }(e.g. Finnigan Magnum) is the mechanism of the Automatic Gain Control if you have a coelution of peaks. }In this case you have troubles with analyzing and quantifiing the minor compound. } }Regards } }Ludwig Gruber } } } } } } Dr. Gruber is exactly right on this. We use both quadrupole systems and an ion trap system (the Finnigan GCQ) in our laboratory for always complex and dirty systems (petroleum and related samples). The ion trap systems have a reputation in our industry of working very well for clean samples at low gc temp (drinking water analysis, etc.), but poorly for complicated dirty systems. The main problem is that there is always tons of stuff eluting (solvent, column bleed, non-analyte-matrix species) that the when the AGC does a "test" ionization to figure out how many ions are present, it always sees a lot, and thus automatically turns its ionization time (sensitivity) down. This means that your detection limits move up and down un-predictably depending on how much is co-eluting in any given sample and you can never guarantee the analyte is not present at any given concentration. The in-trap ionization is also problematic for heavy dirty samples, since for EI, 100,000 neutrals must exit the trap for every ion, which means a lot ends up where you don't want it. The GCQ system mitigates these two problems to some extent, since the ion source is external to the trap, the trap only gets loaded with ions (and background neutrals). It is also possible to use the ms/ms waveform excitation to only count the (limited) analyte mass(es) in the AGC step if you don't need full range spectra. Other manufacturers say they can do this last trick with internal-trap-ionization as well, but I can't speak for how well it works. Other manufacturers say they can treat the trap surfaces with coatings and/or heat to minimize the adverse effects of letting your whole sample (plus column bleed) directly into your analyzer. Hope this helps. Dr. Don Drinkwater dond@mhv.net drinkde@texaco.com drinkde@texaco.com ****************************************************************************** From: rostom@bioch.ox.ac.uk (Adam Rostom) Date: 5 Jan 1998 11:09:18 GMT Subject: Re: peak integration software? Organization: Department of Biochemistry, Oxford University Distribution: world : Other than these two companies, I don't know where else you would find a : package. : You could try Micromass' Mass Lynx software, this has a fairly good peak integration function. Adam ****************************************************************************** From: winnik@charlotte.med.nyu.edu (Witold Winnik, Ph.D.) Date: Mon, 5 Jan 1998 18:11:32 -0500 Subject: service contract Organization: * I would appreciate any advice from magnetic sector VG instrument users about appropriate service companies available on the market. We have got a 10 year old VG-70SE sector instrument are looking for a not very costly service contract that would cover hardware and software of this mass spectrometer. Also, has anybody got any recent experience dealing with or opinions about Research Scientific Services, Inc (RSS; a company that repairs VG sector instruments)? Best Regards, Witold Winnik, Ph.D. New York University Medical Center (914)351-2364 ****************************************************************************** From: "TS" Date: Mon, 5 Jan 1998 20:21:15 -0500 Subject: www.chromtalk.com Organization: * Check out www.chromtalk.com for analytical instrumentation discussion, jobs, gc tips, lc tips, and other useful information. ****************************************************************************** From: "Lewis K. Pannell" Date: Tue, 06 Jan 1998 15:16:55 EST Subject: Facility manager, Mass Spectrometry, NIMH, NIH Organization: * New position announcement: Facility manager, Mass Spectrometry Laboratory of Neurotoxicology, NIMH, Bethesda, MD A new position has been created for an experienced and talented mass spectroscopist (GS-13/14 level, $56-86,000) to supervise an existing mass spectrometry laboratory in the Laboratory of Neurotoxicology directed by Dr. S. P. Markey. The scientist will conduct collaborative research projects with intramural NIMH and NIH scientists, will instruct postdoctoral fellows on state-of-the-art mass spectrometric techniques and operations, as well as direct technical staff in the laboratory. Candidates should hold a Ph.D. degree and have more than five years experience in the field of biomedical applications of mass spectrometry (including protein analysis) and have demonstrated expertise with multiple types of instrumentation.

This position is a term-limited appointment, initially for two years, after which renewed extensions are likely, contingent upon performance and programmatic requirements. Send your c.v. and bibliography, including the names of three references and additional information to: Dr. S.P. Markey Bldg 10-3D40, NIH Bethesda, MD 20892-1262 (fax: 301-480-0198; e-mail: s_markey@codon.nih.gov). ****************************************************************************** From: bhudgens@interaccess.com (Bruce Hudgens) Date: Wed, 07 Jan 1998 12:49:35 GMT Subject: Workshop on February 25 in Chicago Organization: InterAccess Co., Chicago's Full Service Internet Provider Spectroscopy for Process Monitoring and Control A Workshop Sponsored by the Society for Applied Spectroscopy Chicago Section Date: Wednesday, February 25, 1998 Location: Amoco Research Center, 150 W. Warrenville Rd. Naperville, IL Time: 10:00 AM -8:00 PM Tentative Program Topics On-Line and At-Line FTIR On-Line and At-Line NIR Photometers as an alternative to Full Spectral Range Spectrometers On-Line NMR Overview On-Line NMR for Polyolefins Reflectance Measurements Raman for Process Monitoring Gases/Liquids/Solids Sampling Probes for Optical Measurements Reaction Monitoring Process Mass Spectrometry The workshop will provide an overview of spectroscopic techniques used to monitor and control a variety of manufacturing processes. The focus on each of the presentations will be applications driven, rather than technique or technology oriented. An overview on the value of real-time property determinations by spectroscopic measurements will also be presented, along with some of the traps and pitfalls awaiting budding on-line analyzer projects. The goal is for the attendee leave this workshop with a broad understanding of the types of "windows" available to look into their manufacturing processes of interest. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Complete and return to: Myrna Neumann Amoco Corporation 150 W. Warrenville Rd. MS F-5 Naperville, IL 60563 Workshop Pre-Registration Fee - $125.00. Workshop pre-registration for retired chemists/engineers or full time students with a current student I.D is $50.00. The deadline for pre-Registration is February 11, 1998. The charge for late registration is $200.00. There will be no registration at the door. For any questions or additional information regarding the workshop, please contact Bob Roginski at ( 630 ) 420 - 3708 or rtroginski@amoco.com. The Chicago SAS web page is at http://homepage.interaccess.com/~bhudgens/ ****************************************************************************** From: "Lewis K. Pannell" Date: 7 Jan 1998 14:33:24 GMT Subject: ADDENDUM: Facility manager, Mass Spectrometry, NIMH, NIH Organization: * For up to date information, please refer to http://sx102a.niddk.nih.gov/mass/markeyjob.html ****************************************************************************** From: Vimla Jashnani Date: Wed, 07 Jan 1998 10:01:28 -0500 Subject: Post-Doc Available Organization: Applied Physica Laboratory Johns Hopkins University Applied Physics Laboratory Post-Doctoral Position The Johns Hopkins University Applied Physics Laboratory located in Laurel, MD is seeking a qualified individual to participate in the integration of a plasma desorption source with a miniaturized time of flight mass spectrometer as part of a program to build a low power, unattended miniature biosensor. The individual must have experience with the associated instrumentation as well as interest in the mass spectral analysis of biological materials, and an interest in the hands-on construction of a prototype instrument. This is a post-doctoral position with an appointment of 1 year starting in January 1998. For more information, contact Leslie Meyer at JHU/APL (301) 953-6000 ext. 4881 (Leslie.Meyer@jhuapl.edu). For immediate consideration, e-mail to above addresses or fax vitae/resume to (301) 953-5004. Resumes can also be mailed to Johns Hopkins University Applied Physic Laboratory, Johns Hopkins Road, Laurel, MD 20723. U.S. Citizenship required for security clearance purposes. ****************************************************************************** From: William Cotham Date: Wed, 07 Jan 1998 09:34:31 -0500 Subject: Re: service contract Organization: Mass Spectrometry Laboratory You may want to check out GB Scientific in Novato, CA (415) 898-7606. A good bunch of guys IMHO. Witold Winnik, Ph.D. wrote: } I would appreciate any advice from magnetic sector VG instrument users } about appropriate service companies available on the market. We have got a } 10 year old VG-70SE sector instrument are looking for a not very costly } service contract that would cover hardware and software of this mass } spectrometer. Also, has anybody got any recent experience dealing with or } opinions about Research Scientific Services, Inc (RSS; a company that } repairs VG sector instruments)? } } Best Regards, } } Witold Winnik, Ph.D. } New York University Medical Center } (914)351-2364 -- William E. Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry & Biochemistry University of South Carolina Columbia, SC 29208 Phone: (803) 777-2039 FAX : (803) 777-9521 ****************************************************************************** From: "Rolf Brunner" Date: Sat, 10 Jan 1998 14:28:07 +0100 Subject: MM1200 Organization: Unisource Business Networks There is a VG MM1200 equiped with an electrometer Cary 401available. Machine is operating. Contact: robrunner@pop.agri.ch Rolf Brunner ****************************************************************************** From: Doug McIntyre Date: Mon, 12 Jan 1998 17:33:13 -0800 Subject: Re: electrospray source cleaning Organization: Hewlett-Packard ragz@gowebway.com wrote: } } [This is forwarded from sci.chem.analytical. DB] } } A good friend of mine who is a GC/MS chemist will be recieving a HP } electrospray LC/MS soon. Can anyone address the topic of frequency and } difficulty of source cleaning? The current HP LC/MSD requires little routine cleaning even for matrices containing non-volatile materials. It is important to first make a distinction between the spray chamber region and the remainder of the ion optics from the capillary onward which are under vacuum. In the 18 months of using the new LC/MS, I have never had to break vacuum or shut off the pumps for source or analyzer maintenance. The spray chamber, or atmospheric pressure region may need to be cleaned on a frequency as high as daily if running salts or other involatile matrices to every few weeks for volatile matrices. This process takes less than 5 minutes and does not require a system vent. It is the case that as long as the system is under vacuum, we recommend a flow of 3 L/min of nitrogen in order to prevent contaminants from the atmosphere from entering the analyzer. We do not "cap off" the system as we did in the past since we found this caused a migration of pump vapors into the analyzer/source region, ultimately lowering the sensitivity. Doug McIntyre Applications Chemist, Hewlett-Packard company ****************************************************************************** From: foxsteve Date: Tue, 13 Jan 1998 00:43:08 -0800 Subject: Mass spectra for testing software Organization: Netcom Who can help me? Where can I look for the reference with high resolution mass spectra? I need several mass spectra as follows: - for any popular individual compound; it is a good idea to have data for e.g. drugs, pharmaceuticals with MW 300 - 400 Da, with parent and daughter ion clusters. - at high resolution; e.g. R = 50,000 - 100,000. - in list format; M/Z - Intensity. If data have gotten from a GC inlet, I need all scans during one chromatographic peak. If data have gotten for one compounds from direct inlet, I need 10 - 20 scans of data. I am going to use these data as a specific example for testing my software. Steve Ivkov foxsteve@ix.netcom.com ****************************************************************************** From: MasCom@t-online.de (MasCom) Date: 13 Jan 1998 15:56:04 GMT Subject: Used MAT 95 Mass Spectrometer Organization: T-Online We are interested to buy a used MAT 95 mass spectrometer. Please send quotations to: MasCom GmbH Norderoog 1 28259 Bremen Germany Tel: 0049 421 571031 Fax: 0049 421 571032 e-mail: mascom@t-online.de WWW: http://home.t-online.de/home/mascom Thank You ****************************************************************************** From: Caroline Pattenden Date: Thu, 15 Jan 1998 10:17:58 +0000 Subject: MALDI-TOF Organization: The University of Birmingham, UK. Hi I am trying to find the MW of a new polymer I have made, which has hydrocarbon units and aromatic units with ester and anhydride linkages. It is pretty insoluble, so GPC has been no help. I was hoping to try and do some MALDI on my sample, but I am unsure which matrix will be best. If anyone has any ideas for the choice of matrix, and any ideas as to which solvents to try, I would be most grateful if you would let me know. Hope to hear from you soon Caroline Pattenden ****************************************************************************** From: "K.H. Ongania" Date: Thu, 15 Jan 1998 17:09:58 +0100 Subject: 3. IGLER MS-TAGE 1999 Organization: Institute of Organic Chemistry, University Innsbruck I am Happy to inform you that the 3. Igler MS-TAGE will be held from February the 3.-5. 1999 in Igls (Innsbruck/Austria) 3. Igler MS-TAGE "Perspectives in Structural Mass Spectrometry" 3.-5. February 1999 The symposium is organized by the Institute of Organic Chemistry of the University of Innsbruck. Sposership: Spectronex AG BAsel Switzerland and Finnigan MAT http://orgchem1.uibk.ac.at -- ao. Univ. Prof. Dr. Ongania K.H. Institut fuer Organische Chemie Universitaet Innsbruck ****************************************************************************** From: Tom Swenson Date: Thu, 15 Jan 1998 08:23:01 -0800 Subject: WTB: Tekmar, OI instruments Organization: The Internet MainStreet We are looking for LSC-2000's or LSC-3000's and any accessories for Tekmar purge and trap instrumentation. Also looking for OI PID and FID detectors. Please call 800.878.9040 or e-mail sales@spectra-source.com ****************************************************************************** From: "Don Morgan" Date: Thu, 15 Jan 1998 11:57:11 -0700 Subject: FS: Isotope Ratio MS Organization: University of Alberta, Edmonton, Canada Finnigan Mat 252 with Carlo Erba NA 1500 CN Elemental Analyzer -double inlet -six cup collector -changeover valve expansion -Con-Flo Split interface Acquired in 1994, has about 500 hours of use. For Sale or take over lease payments of ~11K ($CDN) till November 1999. Finnigan Mat Breathmat -breath gas autosampler (220 position). Acquired in December 1995, has about 200 hours of use. For sale 40K ($CDN) Contact: Brian Langelier : email blangelier@isotechnika.com Isotechnika Inc. phone (403) 487-1600 17208 108 Ave. fax (403) 484-4105 Edmonton, AB, Canada T5S 1E8 ****************************************************************************** From: "Crockett, Karen (MSMail)" Date: Thu, 15 Jan 1998 16:07:00 -0800 Subject: Interference in VOA GC/MS Analysis Organization: * Help us! We are performing 624 VOA analysis on a HP5972 MSD. The sample we are trying to analyze is a wastewater treatment plant digest with only 1.96% solids. We are purging for 11 minutes, dry purging for 2, then desorbing for 2 minutes. Here's our problem. At the beginning of the run, we get a huge peak with a spectrum that has a peak at every amu (the mass spectrum is basically solid white). The peak causes major sensitivity drops and poor chromatographic behavior (peak splitting). We were told by a HP applications person that the cause of the problem was water in the system. Has anyone seen this, and if so, what was the resolution to the problem? Thanks for any help! Karen Crockett City of Tacoma, WA. (253)502-2132 kcrocket@ci.tacoma.wa.us ****************************************************************************** From: Novbabies Date: Sat, 17 Jan 1998 05:22:01 EST Subject: Explanation of Pumping Speed, Pumping Capacity Organization: AOL (http://www.aol.com) Pumping speed (S) is defined as volume rate of flow of gas through a given aperture or across a given cross-section of the chamber, in e.g. L/sec. How can it be that S increases as headpressure decreases? If there is less available gas to pump, how does the speed increase? Similarly, pumping capacity (Sp) is defined as volume rate of flow of gas at the pressure existing at the inlet to the pump. It also increases as headpressure decreases. How can this be if there are fewer molecules to pump? Is it because now that there are fewer molecules available the capacity of the pump to move more molecules is greater in proportion to what it was when there were a great many molecules? Please help me in my confusion! I will appreciate any response, thanks for the time to help a questing student! Mark - novbabies@aol.com ****************************************************************************** From: "Robert Briggs" Date: Sat, 17 Jan 1998 21:53:28 -0500 Subject: Re: Interference in VOA GC/MS Analysis Organization: AT&T WorldNet Services What is the retention time of this peak? We (at NY State Health Dept., Wadsworth Labs) work with the identical system and see a peak at the onset of each run (maximizing at less than 0.5 min), the intensity of which varies with how clean our source is. This peak results from evaporation of condensate around the filament and is greatly diminished once the source is cleaned and background (which condenses on the surface while the filament is turned off) is eliminated. The peak may be a symptom of your problem rather than the cause of your sensitivity drops. We sometimes do see a water peak which interferes only with toluene (and toluene-d8 surrogate) response but not peak shape. Have you scanned from m/z 15 to confirm presence of water background? We do not work with a matrix such as you describe but it would seem to me that you could encounter foaming which would impact your trap and alter its characteristics, possibly resulting in split chromatographic peaks. Do you monitor the purge cycle to see that this is not happening? bobriggs@worldnet.att.net or robert.briggs@wadsworth.org Crockett, Karen (MSMail) wrote in message <69nn72$2jv@acmex.gatech.edu>... }Help us! } }We are performing 624 VOA analysis on a HP5972 MSD. The sample we are trying to analyze is a wastewater }treatment plant digest with only 1.96% solids. We are purging for 11 minutes, dry purging for 2, then }desorbing for 2 minutes. } }Here's our problem. At the beginning of the run, we get a huge peak with a spectrum that has a peak at }every amu (the mass spectrum is basically solid white). The peak causes major sensitivity drops and poor }chromatographic behavior (peak splitting). We were told by a HP applications person that the cause of }the problem was water in the system. } }Has anyone seen this, and if so, what was the resolution to the problem? } }Thanks for any help! } }Karen Crockett }City of Tacoma, WA. }(253)502-2132 }kcrocket@ci.tacoma.wa.us } } ****************************************************************************** From: Thierry Mann Date: Sun, 18 Jan 1998 10:04:29 -0700 Subject: source cleaning frequency Organization: All USENET -- http://www.Supernews.com I do alot of GC/MS injections with silylating agents . MSTFA, BSTFA, and I find I need to clean the source on a semi-weekly basis due to signal degradation. Of course I also use an autosampler and make ca. 200 injections a week. Does anyone else out there find the need to clean the instrument more often than expected after injecting silyl ethers and esters? ****************************************************************************** From: spiff88@REMOVEMEhotmail.com (Brian Johnson) Date: Mon, 19 Jan 1998 06:11:05 -0700 Subject: Re: Interference in VOA GC/MS Analysis Organization: All USENET -- http://www.Supernews.com In article <69nn72$2jv@acmex.gatech.edu>, "Crockett, Karen (MSMail)" wrote: } Help us! } } We are performing 624 VOA analysis on a HP5972 MSD. The sample we are trying to analyze is a wastewater } treatment plant digest with only 1.96% solids. We are purging for 11 minutes, dry purging for 2, then } desorbing for 2 minutes. } } Here's our problem. At the beginning of the run, we get a huge peak with a spectrum that has a peak at } every amu (the mass spectrum is basically solid white). The peak causes major sensitivity drops and poor } chromatographic behavior (peak splitting). We were told by a HP applications person that the cause of } the problem was water in the system. } } Has anyone seen this, and if so, what was the resolution to the problem? } } Thanks for any help! } } Karen Crockett } City of Tacoma, WA. } (253)502-2132 } kcrocket@ci.tacoma.wa.us Karen - The huge peak at the beginning of the run is most likely water - and methanol from your standards. Depending on the type of purge unit you have, you may have moisture control system (MCS). I know the Teklmar 3000 and the HP labeled Tekmar units have them (I think as standard, and not an option.) Anyway - this peak is not totally avoidable. Remember - you should not start scanning immediately upon injection. You need to set a solvent delay. This delay should last until just before your first compound - usually dichlorodifluoromethane (I think...). Also - with the HP5972 - you must have the same amount of methanol in all your standards and samples. If not, the bromomethane and chloroethane peaks will be suppressed in standards with high methanol content (like 200ppb curve point.) So - figure out the least amount of MeOH you can get away with, and make sure you add 'makeup' MeOH to your samples, blanks, etc. Hope this helped. Brian Johnson AGRA Earth & Environmental (opinions mine) -- remove REMOVEME to email ****************************************************************************** From: Scott Ecelberger Date: Mon, 19 Jan 1998 23:14:10 +0000 (GMT) Subject: Re: Explanation of Pumping Speed, Pumping Capacity Organization: Johns Hopkins Univ. / Applied Physics Lab Distribution: world In article <69qsc5$l3q@acmex.gatech.edu> Novbabies, Novbabies@aol.com writes: }Pumping speed (S) is defined as volume rate of flow of gas through a given }aperture or across a given cross-section of the chamber, in e.g. L/sec. How }can it be that S increases as headpressure decreases? If there is less }available gas to pump, how does the speed increase? You are right in that pumping speed (S) is a volumetric flow rate (L/sec) defined at a point in space - it says nothing about the quantity (number of moles) of gas being pumped unless the pressure (P) is also defined at that point. A liter of gas at 10e-2 Torr is a larger quantity (mass) than a liter of gas at 10e-4 Torr. At higher pressures the pump is less efficient and has to work harder to move a given volume of gas. }Similarly, pumping capacity (Sp) is defined as volume rate of flow of gas at }the pressure existing at the inlet to the pump. It also increases as }headpressure decreases. How can this be if there are fewer molecules to pump? }Is it because now that there are fewer molecules available the capacity of the }pump to move more molecules is greater in proportion to what it was when there }were a great many molecules? I'm not so sure about this statement. Pumping capacity (or gas throughput) is a mass flow rate (Q=P*S). In a compressible gas, this is not the same as a volumetric flow rate. If you look at the pumping speed vs. pressure for a turbo pump, the pumping speed generally levels out to a constant value at pressures below ~10e-3 Torr. The gas throughput (P*S) must drop as head pressure falls if S remains constant. Maybe Q does increase at higher pressures where the pumping speed is climbing with decreasing pressure - I don't know. Hope this helps, Scott ****************************************************************************** From: foxsteve Date: Tue, 20 Jan 1998 02:28:34 -0800 Subject: Re: Interference in VOA GC/MS Analysis Organization: Netcom Crockett, Karen (MSMail) wrote: } } Help us! } } We are performing 624 VOA analysis on a HP5972 MSD. The sample we are trying to analyze is a wastewater } treatment plant digest with only 1.96% solids. We are purging for 11 minutes, dry purging for 2, then } desorbing for 2 minutes. } } Here's our problem. At the beginning of the run, we get a huge peak with a spectrum that has a peak at } every amu (the mass spectrum is basically solid white). The peak causes major sensitivity drops and poor } chromatographic behavior (peak splitting). We were told by a HP applications person that the cause of } the problem was water in the system. } } Has anyone seen this, and if so, what was the resolution to the problem? } } Thanks for any help! } } Karen Crockett } City of Tacoma, WA. } (253)502-2132 } kcrocket@ci.tacoma.wa.us Karen: I think you have unreliable electrical contact on the enter analog signal in analog-digital converter. Steve Ivkov foxsteve@ix.netcom.com ****************************************************************************** From: Steven Cepa Date: Tue, 20 Jan 1998 10:41:28 -0600 Subject: LC/MS mobile phases Organization: Abbott Laboratories We have recently been getting a bunch of our in-house customers asking for LC/MS on systems that they have developed using perchloric acid as the mobile phase modifier. I have tried substituting with TFA or formic acid, but this often does not result in acceptable chromatography. Has anyone tried perchloric acid with APCI or ESI? Does anyone have acceptable substitutes? These are generally classic organic molecules with acetonitrile: acidified aqueous gradients. The instrument is an LCQ. -- ********************************* % Knowledge is a process of piling up facts; % wisdom lies in their simplification. Steve Cepa Abbott Labs, North Chicago, IL ****************************************************************************** From: Joachim Greter Date: Wed, 21 Jan 1998 10:38:06 +0100 Subject: Re: Frequent source cleaning - TMS derivatives Organization: Centrallaboratoriet för Klinisk Kemi, SU/Sahlgrenska, 413 45 Göteborg, Sweden }Thierry Mann wrote: } } I do alot of GC/MS injections with silylating agents . MSTFA, BSTFA, and } I find I need to clean the source on a semi-weekly basis due to signal } degradation. Of course I also use an autosampler and make ca. 200 } injections a week. Does anyone else out there find the need to clean the } instrument more often than expected after injecting silyl ethers and } esters? We do about 100 silylated (BSTFA) GC-MS samples a week and clean the source once a month in average because of sensitivity deterioration. We even clean the prefilters (MD800 instrument) about every six months because of deteriorating transmission and stability. After about one to one and a half year we replace the entire quadrupole analyzer in order to regain acceptable transmission and stability. (Capillary column used: 50 m, 0.5 mm ID, 1 =B5m crosslinked methyl-phenyl silicone). I have been told that laboratories doing doping analysis (horse racing and other) with silylated samples have similar experiences. So, go on cleaning, you are not alone! Cheers, Joachim Greter ****************************************************************************** From: "Denis LOYAUX" Subject: MS/BIO Post Doctoral position Date: Tue, 20 Jan 1998 22:28:35 +0100 Organization: Ye 'Ol Disorganized NNTPCache groupie [This is forwarded from sci.techniques.spectroscopy. DB] SYNTHELABO, France's 3rd largest pharmaceutical group, which employs 8300 people, has achieved a turnover of 10,4 thousand million francs in 1996, 1,5 thousand million of which is invested in its research activities. The RESEARCH DIVISION, with its staff of 1350 specialised researchers, develops highly innovative drugs, mainly in the areas of central nervous system, cardiovascular and internal medicine. We have currently a major commitment in the domain of genomics-based pharmaceutical research as a result of recent alliances with Human Genome Sciences / SmithKline Beecham and Genset. We are looking for a: MASS SPECTROMETRIST PROTEOMICS Post Doctoral Position (réf. DL/MS) As part of our multi-disciplinary MS, NMR and X-RAY team in the Biomolecular Chemistry Group, you will be expected to develop methods to separate, characterise and identify proteins from various known or unknown genes through protein and EST public/private data bank interrogations, developing 2D gel electrophoresis / MS and MS / MS techniques. To qualify for this one-year post-doctoral fellowship, you must have a PhD in Biophysics / Biochemistry / Biology coupled with a strong background in protein biochemistry and peptide sequencing by ESI / MS / MS. An experience in state-of-the-art MS techniques (MALDI / MS and NanoESI / MS / MS) to investigate proteomes would be a great advantage. This position is located in RUEIL-MALMAISON, a western suburb of Paris. Knowledge of French is not essential but would be an advantage. If you are interested, please forward your application (C.V. and covering letter) quoting the job reference to Dr Denis LOYAUX Synthélabo Recherche Biomolecular Structural Chemistry Group 10 rue des Carrières 92500 RUEIL-MALMAISON - France. Or send a resume (text or Word file) and references to this E-mail address: dloyaux@infonie.fr ****************************************************************************** From: Mike Greig Date: Wed, 21 Jan 1998 08:43:13 -0800 Subject: Job Posting Organization: * Alanex Corp., a San Diego combinatorial drug discovery company, has an immediate opening for an analytical chemist/mass spectrometrist with PhD or equivalent experience. Candidates will provide structural characterization and determine purity of combinat orial compounds as well as investigating interactions between small molecules and biological targets. Experience with ESI, APCI, LC/MS, and maintenance of instruments a must. Additional experience with GC/MS, high-throughput automation, biological purif ication and mass spec, and ion traps desirable. Interested applicants please send resume to: Mike Greig at Alanex Corp., 3550 General Atomics Ct., San Diego, CA 92121-1194. Michael Greig Alanex Corp. 3550 General Atomics Ct. San Diego, CA 92121-1194 (619) 455-3241 mjg@alanex.com ****************************************************************************** From: "Michael O'Donnell" Date: Thu, 22 Jan 1998 16:32:09 +0800 Subject: Technology Updates Organization: ITX I thought that the people on this news group might like to know about a new news letter we are sending out every two weeks. It covers all aspects of technology and feidsl of endevour. We now have over 350 listed new technologies. ------ TECHNOLOGY UPDATES ------ VOL 1 ISSUE 1 Welcome to the first issue of "Technology Update". Here is how it works. Every two weeks you will receive an email with four of the latest technologies that have been posted to our site plus the latest addition to our Wurlds Best Ideas section. IMPORTANT NOTICE SEE BOTTOM OF EMAIL All you will have to do is click on the link to view the full detail. WURLDS BEST IDEA'S http://www.technologyxchange.com/wbi/index.html ASTHMA - NOVEL TARGETS FOR THERAPY http://www.technologyxchange.com/quick-search.cgi?tech=884564491&command=View CASTANOSPERMINE http://www.technologyxchange.com/quick-search.cgi?tech=884560264&command=View SIDS http://www.technologyxchange.com/quick-search.cgi?tech=884562917&command=View NOTICE If you wish to continue receiving our fortnightly e-mail you will need to send an email to tech_updates@technologyxchange.com with the word Subscribe in the body of the text or in the subject heading. If you do not subscribe you will not receive any further issues of "Technology Update". Please visit the site and search the database. The site address is http://www.technologyxchange.com ****************************************************************************** From: Steffen Thomas Date: Thu, 22 Jan 1998 15:11:42 +0100 Subject: Update at www.chem.uni-potsdam.de Organization: University of Potsdam HAllo, trough any security holes in our old server software and the publishing of this in many news- groups and lists, we must update our www server! A T T E N T I O N Some URL's has changed from / to /nmr_home directory. The spectrsocopic Wizards also has a new home- Page at http://www.chem.uni-potsdam.de/wizard.htm Please update our bookmarks!! Thank You for your attention. St. Thomas -- **************************************************************** Laechle und sei froh, denn es koennte viel schlimmer kommen. Ich laechelte und war froh und siehe da, es kam schlimmer. **************************************************************** ****************************************************************************** From: ricksfbrsc@aol.com (Ricksfbrsc) Date: Thu, 22 Jan 1998 19:34:17 -0500 Subject: Re: Interference in VOA GC/MS Analysis Organization: AOL http://www.aol.com It sounds like water to me, also. You can test if its water by: 1. Inject a standard into purge vessel with no water--see if problem goes away. 2. Increase the dry purge time to say 5 min.--see if problem goes away or is less. 3. Purge for less time (efficiency will be reduced, but you will have less water in system). Also, check to see if you have gotten a bad tank of carrier gas and/or whether water traps in line are saturated and need to be baked out. Good luck! ****************************************************************************** From: "Kai Fauth" Date: 23 Jan 1998 08:15:51 GMT Subject: QMS - high mass range Organization: University of Wuerzburg, Germany We are currently planning to build a research system a major part of which will probably be a quadrupole mass filter aimed at the range from some tens to several thousands of amu's (externally created ions), not for detection only but also for surface deposition experiments. Currently we have informations from EXTREL and HIDEN as competitors only. Could you share experiences with these suppliers or suggest other competitors to consider? Thanks for help to a newcomer... -- Dr. Kai Fauth Experimentelle Physik IV Universität Würzburg Am Hubland D-97074 Würzburg fauth@physik.uni-wuerzburg.de ****************************************************************************** From: john@wd1v.mv.com (John Seney) Date: Fri, 23 Jan 1998 07:11:40 -0400 Subject: Pointer to Scope.FAQ Organization: WD1V Oscilloscope.FAQ is located on my home page. You need a frames capable browser to see it. If you'd like an Email version sent to you as an attached text file (55k), send an Email with SCOPE FAQ on the SUBJECT LINE. Best regards, John Seney http://www.mv.com/ipusers/wd1v Oscilloscope.FAQ ****************************************************************************** From: Kurt Benkestock Date: Fri, 23 Jan 1998 14:23:50 +0100 Subject: Interpretation of peptides fragment ion data... Organization: Pharmacia & Upjohn Hi! I'm looking for courses in de novo MS/MS spectral interpretation of peptides. Could someone give me information about when, where and possible web-address of such courses. Thanks in advance! Regards, Kurt Benkestock Pharmacia & Upjohn Structure Chemistry Mass Spectrometry Group 112 87 STOCKHOLM SWEDEN E-mail: kurt.benkestock@eu.pnu.com Phone: +46 8 695 7138 Fax: +46 8 695 4082 ****************************************************************************** From: Dan Heglund Date: Fri, 23 Jan 1998 12:59:19 -0700 Subject: WTB and opinions on uses GC/MS Organization: South Dakota School of Mines and Technology All- Does anyone have any opinions on the purchase of a used GC/MS? I just started teaching at the SD School of Mines and I would like to buy a used GC/MS. I really don't need the greatest detection limits, just a general purpose instrument. If I publish the results, I can always state that detection limits could be improved by.... I have found several people on the www that would like to sell me an instrument. I'm currently limited to about 20k as my price. Several people have a HP 5890A GC with a 5907B MSD for 20k. This price includes the computer, DOS software, GC and MSD. Any opinions would be appreciated. Dan Heglund SD School of Mines Dept of Chem/ChemE 501 E. St Joseph St Rapid City, SD 57701 (605) 394-1241 ****************************************************************************** From: "c. stader" Date: Fri, 23 Jan 1998 12:26:16 -0700 Subject: R&D lab selling TOF-MS, turbomolecular drag pump and microwave digestion oven Organization: MailExcite (http://www.mailexcite.com) From: EASTERN ANALYTICAL, INC. 211 PERRY PARKWAY, SUITE 5 GAITHERSBURG, MD 20877 Tele:301-330-4155//FAX:301-330-6976//E-Mail:catherines@mailexcite.com If you would like more information, please contact EAI (contact information is shown at the top of this message). FOR SALE: 1) Laser Ionization One Meter Linear Time-of-Flight Mass Spectrometer with pulsed molecular beam source (Comstock model LTOF-110-LI) In use for one year. asking $25,000 2) Turbomolecular Drag Pump (Pfeiffer Vacuum Technology Model TMU260) in use for one year. asking $5,250 3) Microwave Digestion Oven (CEM model SP8 MDS-2000) Less than 40 hours of use, no chemical digestions performed. asking $12,000 Free web-based email, Forever, From anywhere! http://www.mailexcite.com ****************************************************************************** From: Fred Rueggeberg Date: Sat, 24 Jan 1998 20:45:15 -0500 Subject: Need Spectrum Organization: CSRA Internet Services I am reviewing a manuscript, and would greatly appreciate if someone could supply me with a Mass Spec of Bisphenol-A. Thanks inadvance Fred ****************************************************************************** From: David Sparkman Date: Sun, 25 Jan 1998 22:49:51 -0500 Subject: Protein & Peptide Course Organization: * Kurt Benkestock wrote: }I'm looking for courses in de novo MS/MS spectral interpretation of }peptides. Could someone give me information about when, where and }possible web-address of such courses. Thanks in advance! The ACS is offering two sessions of a hands-on course in the analysis of peptides and proteins by mass spectrometry this summer at Michigan State University (East Lansing, MI). The couse will start at the beginning of how to perpare samples and deal with the resulting data. This includes MS/MS ESI data and MALDI post-source decay data. This is a lab and lecture course with a lot of homework. The course is 5 days. For details, call the Am Cem Soc at 1-800-227-5558 and ask for the Continuing Education Department or check the Con Ed section of http://www.acs.org. Regards; David Sparkman Consultant-At-Large 510-754-5003 ****************************************************************************** From: "Don Morgan" Date: Mon, 26 Jan 1998 15:37:44 -0700 Subject: FS: MAT 250 IR MS Organization: University of Alberta, Edmonton, Canada Finnigan Mat 252 with Carlo Erba NA 1500 CN Elemental Analyzer -double inlet -six cup collector -changeover valve expansion -Con-Flo Split interface Acquired in 1994, has about 500 hours of use. For Sale or take over lease payments of ~11K ($CDN) till November 1999. Finnigan Mat Breathmat -breath gas autosampler (220 position). Acquired in December 1995, has about 200 hours of use. For sale 40K ($CDN) Contact: Brian Langelier : email blangelier@isotechnika.com Isotechnika Inc. phone (403) 487-1600 17208 108 Ave. fax (403) 484-4105 Edmonton, AB, Canada T5S 1E8 ****************************************************************************** From: "bdguest" Subject: GC/MS Instrument Evaluations Date: 27 Jan 1998 20:59:39 GMT Organization: Insync Internet Services [This is forwarded from sci.chem.analytical. DB] I'm considerin purchasing a GC/MS. What do you know about the reliability and sensitivity of the Shimadzu, Finnigan, and Hewlett-Packard instruments? Would appreciate any feedback. I would be buying a new instrument, so only supply info on the latest models please. Dr. B. ****************************************************************************** From: efisher@cc.curtin.edu.au Date: Wed, 28 Jan 1998 06:34:18 GMT Subject: Balzers Thermostar 422 Process Control Module Organization: Curtin University of Technology Hello, We've got a BalzersThermostar mass spectrometer hooked up to a thermal analysis instrument. Currently I am looking into using the process control module so I can create a sequence. The manual that comes with the BalzersThermostar mass spectrometer has alot of information on what is possible to program sequences, but not alot on definitions. Before I spend hours trying to figure it all out, does anyone have experience in this area or pointers to good English references. This will make my life a little easier. Nick Fisher. ****************************************************************************** From: gal@unice.fr (J.-F. Gal) Date: Wed, 28 Jan 1998 08:48:20 +0000 Subject: Mass spectra of amino acid derivatives Organization: * Hi MS people ! I'd like to know if anyone knows a good source of spectra of aminoacid derivatives (N-acetyl alkyl esters), like those used for GC/MS, particularly GC/C/IRMS. Your help will be highly appreciated. Jean-Francois Gal -------------------------------------------------------- Prof. J.-F. GAL Groupe FT-ICR-GRECFO-Chim. Phys. Org. Fac. des Sciences-Parc Valrose Univ. de Nice-Sophia Antipolis 06108 NICE Cedex 2-FRANCE Tel : foreign (33) 492 07 61 10; domestic 04 92 07 61 10 Fax : foreign (33) 492 07 61 11; domestic 04 92 07 61 11 E-mail : gal@unice.fr -------------------------------------------------------- ****************************************************************************** From: Vladimir Elias Date: Wed, 28 Jan 1998 15:32:01 -0800 (PST) Subject: Re: Need Spectrum Organization: * Fred Rueggeberg said >I am reviewing a manuscript, and would greatly appreciate if someone >could supply me with a Mass Spec of Bisphenol-A. > >Thanks inadvance > >Fred > The MS of Bisphenol-A can be retrieved from the Wiley library. Entries 114524 and 114525 of Wiley275.L. If you donUt have access to this library I can send you a doc file so you can read in any Word processor. Just drop me an Email if you need it. Anyway, I put below the mass abundances of Bisphenol-A. m/z abund. 89.00 395 90.00 395 91.00 4866 92.00 395 96.00 395 98.00 789 107.00 1580 119.00 9999 120.00 1053 134.00 2368 135.00 2368 151.00 132 152.00 263 165.00 789 213.00 7108 214.00 1136 215.00 113 228.00 4605 229.00 790 ******************************************************* Vladimir O. Elias www.orst.edu/~eliasv Oregon State University College of Oceanic and Atmospheric Sciences 104 Ocean Admin Bldg Tel: 541-737-2741 Fax: 541-737-2064 ******************************************************* ****************************************************************************** From: Vladimir Elias Date: Wed, 28 Jan 1998 15:58:23 -0800 (PST) Subject: Reply to stm-s Organization: * Response to: >I'm considerin purchasing a GC/MS. What do you know about the >reliability >and sensitivity of the Shimadzu, Finnigan, and Hewlett-Packard >instruments? >Would appreciate any feedback. I would be buying a new instrument, so >only supply info on the latest models please. > >Dr. B. I donUt know about Schimadzu and Finnigan but the new HP 5973 MSD is a powerful and impressive machine. IUve work on the previous models (5987, 5970, 5971, 5972) and can tell you that this new model is superior in many aspects. IUve been using the HP 5973MSD for 13 months and IUm impressed with its performance (so far!!!). The results are reproducible (even at trace levels as pg), the source is easy and quick to be cleaned (2 hours at most) and donUt so many cleaning as the previous models. Cleaning is usually required prior injections of more than 200 silylated samples. The system is also very friendly allowing first time users not so skilled operators to maintain the machine. A CD-ROM video shows step-by-step what needs to be done. An advantage in educational institutions always where students are always coming, learning and going. In my opinion the only disadvantage is when we do high temperature GCMS. As I mention in my recent papers (JMS and HRC), in this system we can just see masses up to 800 uma. As during HT-GCMS many compounds exceed the 1000uma this the limitation of 800uma s not acceptable. But I think HP will have to change this because HTGCMS is growing pretty fast. VOE ******************************************************* Vladimir O. Elias www.orst.edu/~eliasv Oregon State University College of Oceanic and Atmospheric Sciences 104 Ocean Admin Bldg Tel: 541-737-2741 Fax: 541-737-2064 ******************************************************* ****************************************************************************** From: Gary Radford Date: Wed, 28 Jan 1998 21:46:50 -0500 Subject: Re: Interference in VOA GC/MS Analysis Organization: Radford Scientific Could be water but you're lucky you have an MS to investigate what it is Try decreasing the EM electron multiplier voltage by about 200 to 400 and set the scan range 10 to 300. Water should give you 18. Air wil be 28 and 32. Methanol 31. You should be able to also library search to identify the interference. Crockett, Karen (MSMail) wrote: } Help us! } } We are performing 624 VOA analysis on a HP5972 MSD. The sample we are trying to analyze is a wastewater } treatment plant digest with only 1.96% solids. We are purging for 11 minutes, dry purging for 2, then } desorbing for 2 minutes. } } Here's our problem. At the beginning of the run, we get a huge peak with a spectrum that has a peak at } every amu (the mass spectrum is basically solid white). The peak causes major sensitivity drops and poor } chromatographic behavior (peak splitting). We were told by a HP applications person that the cause of } the problem was water in the system. } } Has anyone seen this, and if so, what was the resolution to the problem? } } Thanks for any help! } } Karen Crockett } City of Tacoma, WA. } (253)502-2132 } kcrocket@ci.tacoma.wa.us ****************************************************************************** From: PAREESDM@apci.com Date: Wed, 28 Jan 1998 22:37:35 -0500 Subject: re: need spectrum Organization: * }From: Fred Rueggeberg }Date: Sat, 24 Jan 1998 20:45:15 -0500 }Subject: Need Spectrum }Organization: CSRA Internet Services }I am reviewing a manuscript, and would greatly appreciate if someone }could supply me with a Mass Spec of Bisphenol-A. }Thanks in advance }Fred The spectrum of bis-phenol A [4,4'-(1-methylethylidene)bis-phenol] is fairly simple and straightforward, consisting largely of a reasonably intense molecular ion (perhaps 30-40%) and the base ion is M-methyl (m/z 213). other significant ions include m/z 135, 119 and 91, with numerous other lower intensity ions, many below m/z 91 and typical of aromatic compounds. Dave Parees My e-mail responses are not the responsibility of my employer. ****************************************************************************** From: J.Gormally@chemistry.salford.ac.uk (John) Date: Thu, 29 Jan 98 15:26:38 GMT Subject: Lasers and Optics (WWW page) Organization: UOS Lasers & Optics is at: http://members.aol.com/WSRNet John ****************************************************************************** From: pcs22@cam.ac.uk Subject: Mass Spectrometry Vacancy Date: Thu, 29 Jan 1998 06:33:33 -0600 [This is forwarded from sci.techniques.spectroscopy. DB] UNIVERSITY OF CAMBRIDGE DEPARTMENT OF CHEMISTRY Mass Spectrometry Technician T4 £12654-£15565 (Limit of Tenure of March 2002) Job No: OI59A A technician is required to operate mass spectrometers to provide a chemical analysis service to research groups in the Department of Chemistry (rated 5* in the 1996 Research Assessment Exercise), other departments within the University and researchers in outside commercial companies. The appointee will join a large friendly section, the work being varied and interesting. He/she will gain experience in the analysis of a wide range of chemical and biological samples using different ionization methods as well as gas and liquid chromatography. This post would suit a person who has a knowledge of chemistry and has either had relevant experience, or experience with similar or related equipment who wishes to gain knowledge of this specialized field, and who is interested in using computers to control machine operation and manipulate data output. Training will be given if necessary. Trainees will be considered. Smoking is not permitted in the Department. Please send an application and CV with two nominated referees to: The Technical Secretary, Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW. The University is an equal opportunities employer. -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet ****************************************************************************** From: David Sparkman Date: Sat, 31 Jan 1998 08:23:42 -0500 Subject: Mass Spectrum of Bisphenol-A Organization: * Fred Rueggeberg requested a source of the mass spectrum of Bisphenol-A. Vladimir O. Elias submitted a spectrum taken from the HP version of the Wiley 6N database with the base peak at m/z 119. This is the mass spectrum for 2,4'-Bisphenol-A (a.k.a. as the O P isomer of Bisphenol-A). Bisphenol-A usually refers to 4,4'- or the P P isomer of the compound. As shown in the NIST'98 Main Database of 108K compounds, 2,2-(4,4'-dihydroxydiphenyl) propane has a base peak of 213. This is confirmed by the two spectra in the NIST'98 Selected Replicates Database. These spectra are consistent with the information submitted by Dave Parees which stated that the compound has a base peak at m/z 213 and the molecular ion peak is 30 to 40% of that and other significant peaks at m/z 135, 119, and 91. The NIST'98 Mass Spectral Database is now being distributed by HP in the NIST98.L format and in the NIST format along with the NIST Mass Spectral Search Program for Windows V 1.6. The NIST 98 Mass Spectral Database and V.16 of the Search Program are also available from ChemSW in Fairfield, CA and Standard Reference Data at NIST in Gaithersburg, MD. This new version of the NIST MS Search Program is interactive with both the HP MSD (version C.3.00.00 and higher) ChemStation and the Finnigan GCQ. It also allows for the importation of MOL file structures into User libraries. Both ChemSW and SRD/NIST have an upgrade path for current users of the NIST MS Search program. The toal number of compounds in this new Databse from NIST is approx 108K and the total number of spectra is approx 130K. There are only 57 fewer structures than spectra in the NIST'98 Database and every spectrum has been evaluated by two mass spectrometrist. This Mass Spectral Database has half again as many peaks as any other mass spectral collection available in electronic format, regardless of spectral size. There is an average of 93 peaks/spectrum. O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: renee10195@aol.com (Renee10195) Date: Sun, 1 Feb 1998 15:36:41 -0500 Subject: Bromide interference Organization: AOL http://www.aol.com I am analyzing for Selenium mass 82 and have a Bromide interference. Any suggestions on how to correct? Please e.mail direct. ****************************************************************************** From: "Thomas Eßer" Date: Sun, 1 Feb 1998 21:53:47 +0100 Subject: spectrum of O2, CO2, N2, He Organization: Metronet Hello, I´m writing my theses in the diving department of draeger dive industries. In need help finding the spectrum of O2, He, N2, CO2 on the web. Has anybody a good link for me. Previously a lot of thanks. e-mail: Thomesser@metronet.de ****************************************************************************** From: Greg Grinstead Date: Sun, 01 Feb 1998 19:14:11 CST Subject: Drifting ion ratios with HP 5970 MSD Organization: * I have an HP 5970 quadrupole MSD (vintage 1991) which is used for workplace and clinical drug testing. We use electron impact ionization with selected ion monitoring for detection and quantitation of drugs of abuse. Typically we inject a cutoff calibrator and acquire a quantitating ion and two qualifying ions for each analyte. For identification of unknowns we require that the qualifying ion abundance ratios be within 20% of the abundance ratio obtained in the calibrator injection. Until about two years ago, the ion abundance ratios obtained within a sequence of injections on the same day were very stable. My problem: We now have a vexing problem with unstable or "drifting" ion abundance ratios. That is, we inject a calibrator, establish acceptable ranges for the abundance ratios, then proceed to inject controls and unknowns. With each succeeding injection, qualifying ion abundance ratios creep toward the limit of the acceptable range and eventually go outside the 20% limit. In order to meet regulatory requirements we then have to reinject calibrator, controls, and affected samples -- and hope that the ratios stabilize. The problem occurs more frequently at relatively high masses (200 - 625 amu). It does not occur in every sequence of injections, but does occur probably two out of every three days, and causes us real problems. We do regular maintenance and ion source cleaning, but the quadrupole and most of the ion source parts are original equipment. We have not used a muffle furnace to clean the ceramic components. Replacing the electron multiplier did not help. HP Technical Support and our HP service rep have been unable to diagnose the problem or offer suggestions. If anyone has encountered a similar problem and found a solution, I'd very much appreciate hearing from you. Greg Grinstead Marshfield Laboratories Marshfield, WI grinstead@tznet.com ****************************************************************************** From: "magnar ervik" Date: 2 Feb 1998 12:11:05 GMT Subject: FOR SALE: Used Finnigan MAT95 Organization: Astra AB GC-MS system consisting of a Hewlett-Packard model 5890 GC coupled to a Finnigan MAT95 mass spectrometer. The GC is supplied with a A 200 S autosampler. The MS is equipped with alternating EI/CI ion source, desorption chemical ionization (DCI) probe,liquid cooled rapid heating direct insertion probe and heated reference inlet The data system is a DEC Station 5000/120-32 MB, with added storage memory (totally 1.1 GB) provided with a streamer tape drive as well as a CD drive. Astra Hdssle AB Bioanalytical Chemistry S-431 83 Mvlndal Sweden Tel: +46 31 776 13 30 Fax: +46 31 776 37 11 E-mail: magnar.ervik@hassle.se.astra.com ****************************************************************************** From: Thierry Mann Date: Mon, 02 Feb 1998 07:47:26 -0700 Subject: Re: Drifting ion ratios with HP 5970 MSD Organization: All USENET -- http://www.Supernews.com Greg Grinstead wrote: } } I have an HP 5970 quadrupole MSD (vintage 1991) which is used for workplace } and clinical drug testing. We use electron impact ionization with selected } ion monitoring for detection and quantitation of drugs of abuse. Typically } we inject a cutoff calibrator and acquire a quantitating ion and two } qualifying ions for each analyte. For identification of unknowns we require } that the qualifying ion abundance ratios be within 20% of the abundance } ratio obtained in the calibrator injection. Until about two years ago, the } ion abundance ratios obtained within a sequence of injections on the same } day were very stable. } } My problem: We now have a vexing problem with unstable or "drifting" ion } abundance ratios. That is, we inject a calibrator, establish acceptable } ranges for the abundance ratios, then proceed to inject controls and } unknowns. With each succeeding injection, qualifying ion abundance ratios } creep toward the limit of the acceptable range and eventually go outside the } 20% limit. In order to meet regulatory requirements we then have to reinject } calibrator, controls, and affected samples -- and hope that the ratios } stabilize. The problem occurs more frequently at relatively high masses (200 } - 625 amu). It does not occur in every sequence of injections, but does } occur probably two out of every three days, and causes us real problems. We } do regular maintenance and ion source cleaning, but the quadrupole and most } of the ion source parts are original equipment. We have not used a muffle } furnace to clean the ceramic components. Replacing the electron multiplier } did not help. } } HP Technical Support and our HP service rep have been unable to diagnose the } problem or offer suggestions. If anyone has encountered a similar problem } and found a solution, I'd very much appreciate hearing from you. } } Greg Grinstead } Marshfield Laboratories } Marshfield, WI } grinstead@tznet.com hmm, I wonder if you use BSTFA w/ 1% TMCS as a derivatizing agent? I think you need to consider column degradation as a source of qualifier ion instability. If the column is showing bleed at m/z say 174 and that is one of your ions monitored, then that may "screw up" your ratios, especially if the bleed is increasing with injections. Try conditioning the column prior to doing the actual runs. make 5 injections of blank then start the calibrators. hope that helps, and if you are using silylating agents, try switching to MSTFA instead, it is much less harsh on the instrument. ****************************************************************************** From: "PatkinAJ" Date: Mon, 02 Feb 98 12:23:55 EST Subject: Need NCI spectrum for tris(perfluoroheptyl)-s-triazine Organization: * I am looking for a negative chemical ionization (preferably methane) reference spectrum for tris(perfluoroheptyl)-s-triazine. Can anybody out there help? Thanks. Adam Patkin patkinaj@perkin-elmer.com The Perkin-Elmer Corporation Tel: (203) 761-2908 Fax: (203) 761-2887 ****************************************************************************** From: "Chemie.DE" Date: Mon, 02 Feb 1998 19:29:12 +0100 Subject: Calendar of Chemical Events Organization: Chemie.DE Information Service Ladies and Gentleman, Chemie.DE (http://www.chemie.de) is a German information service on the internet offering organized data in the fields of chemistry, biochemistry and related sciences. It is a project funded by the German Verein Deutsches Forschungsnetz (DFN) and authorized by the German Ministry of Education, Science and Technology (BMBF). Under this project a database of international events in chemistry is now provided for ready reference. * The calendar contains worldwide conferences, meetings, symposiums, workshops, seminars, trainings, exhibitions, and shows related to chemistry * It is internet's most comprehensive calendar of chemical events, containing more than 1000 up to date events * A search engine enables the user to search the events specifically for countries, subjects, dates and details * The user is free to enter events himself * The use of this calendar is free of charge You are kindly asked to make use of and up-date this free calendar in order to keep the international community of chemists well informed of events organized by you or your organization. You'll find the calendar of chemical events at http://www.chemie.de/conferences/ If you have questions, please contact me at knecht@chemie.de Yours Sincerely, Stefan Knecht Chemie.DE Team ________________________________________________________________________ Chemie.DE Information Service http://www.chemie.de/ Freie Universtitaet Berlin mailto:www@chemie.de Fachbereich Chemie Tel +49 (0)30 8383408 Takustrasse 6, D-14195 Berlin Fax +49 (0)30 8383464 ________________________________________________________________________ ****************************************************************************** From: dhachey@bcm.tmc.edu (David Hachey) Date: Mon, 02 Feb 1998 20:21:24 GMT Subject: Re: Drifting ion ratios with HP 5970 MSD Organization: Baylor College of Medicine, Houston, Tx Greg; Your problems sound like a classical case of space charging. In this type of problem some element in the ion optic path has become contaminated and when exposed to the ion beam, it builds up a charge and deflects the major beam, especially high mass ions. We see this problem fairly often with our HP instruments, and the only soution has been to clean the ion entrance lens and the quadrupoles themselves. This usually restores high mass ion transmission. A way to diagnose the problem is to repetitively inject the same reference compound (over a weekend) and look for a gradual change in relative ion abundances. I've seen problems of this sort in the ion source, quad, and electron multiplier (X-ray shield), so it can be a tough problem to sort out. Call HP and see if they can offer any advice, in 30-yr of MS, I've learned that some service engineers are much better than others and are worth their weight in gold. Good luck, Dave Hachey On 2 Feb 1998 08:15:35 -0500, Greg Grinstead wrote: } }I have an HP 5970 quadrupole MSD (vintage 1991) which is used for workplace }and clinical drug testing. We use electron impact ionization with selected }ion monitoring for detection and quantitation of drugs of abuse. Typically }we inject a cutoff calibrator and acquire a quantitating ion and two }qualifying ions for each analyte. For identification of unknowns we require }that the qualifying ion abundance ratios be within 20% of the abundance }ratio obtained in the calibrator injection. Until about two years ago, the }ion abundance ratios obtained within a sequence of injections on the same }day were very stable. } [Snip, snip] } }Greg Grinstead }Marshfield Laboratories }Marshfield, WI }grinstead@tznet.com } } } ****************************************************************************** From: Jens Griep-Raming Subject: CID spectra of (MH)+-ions of carboxylic acids Date: Mon, 02 Feb 1998 19:55:11 +0100 Organization: Universitaet Oldenburg [This is forwarded from sci.chem.analytical. DB] I found that doing MS/MS-analysis of the (MH)+-ions of an carboxylic acid (e.g. valeric acid) generates (MH-18)+-ions (which is clear to me) and also (MH-28)-ions. As an example the CID-spectrum of the (MH)+-ion of valeric acid is given: m/z 103 85% (MH)+ protonated molecular ion m/z 85 6% (MH-18)+ loss of water m/z 83 4% m/z 75 100% (MH-28)+ Does anyone know a raction-pathway that leads to a loss of 28 amu from an protonated carboxylic acid? Any literature to this subject? Ions were generated by Electrospray (ES) spraying a solution of the acid solved with methanol/water 1:1 with 1% acetic acid. The resulting spectrum consisted mainly of the (MH)+-ion. The (MH)+-ions were stored in a quadrupole ion trap and then fragmented by collision induced dissociation (CID). Steath gas was nitrogen, buffer gas was helium. Any comments are welcome. Jens Griep-Raming -- ********************************** Jens Griep-Raming Universität Oldenburg FB 9 Chemie AG Metzger / Massenspektrometrie Postfach 2503 26111 Oldenburg Germany griep@chemie.uni-oldenburg.de Tel. +49 441-798-3731 ********************************** ****************************************************************************** From: Jennifer Loreti Date: Mon, 2 Feb 1998 15:13:45 -0500 Subject: Job Posting Organization: * PRODUCT DEFINITION SCIENTIST If your expert knowledge of Mass Spectrometry, specifically LCMS with Quadrupole and Time of Flight Instruments, is demonstrated by a history of research publications and presentations at major scientific conferences ---- then, have we got a challenge for you! This key role involves designing, building, testing and introducing novel scientific instruments to the mass spectrometry market. You will draw on your solid background and experience in science, engineering, manufacturing, marketing, and customer service. A Degree in Analytical Chemistry, Physics and/or Biochemistry, preferably at the PH.D. level, is required. Our successful candidate combines excellent organizational and interpersonal skills with an ability to generate research-grade literature publications and perform technical presentations at conferences and customer sites. Please forward a detailed resume to the attention of: Human Resources, MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada L4K 4V8 or Fax (905) 660-2600. E-Mail: careers@sciex.com PROTOTYPE SCIENTIST Responsibilities: You will be responsible for developing prototype software for new applications of mass spectrometry. The main duties include determining the user requirements and designs, implementing, testing and documenting a prototype solution. Protoypes may be implemented as AppleScripts, Scripting Additions, C++ programs or VisualBasic programs. You may also modify existing modules to further refine or tailor them to the needs of individual customers. Qualifications: Either a Bachelor or Science in Chemistry, Applied Chemistry or Analytical Chemistry with three years experience of programming, Or: Bachelor of Science in Computer Science with experience in the development of software for chemical instrumentation, chemistry or biochemistry. Willing to travel in North America and Europe. Good communication skills are a must. The ability to explain software capabilities to non-programmers, to understand chemical and biological applications, and to give presenations on applications to mixed audiences. Please forward a detailed resume to the attention of: Human Resources, MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada L4K 4V8 or Fax (905) 660-2600. E-Mail: careers@sciex.com ****************************************************************************** From: "Rich Suthard" Date: Mon, 2 Feb 1998 16:10:43 -0800 Subject: Re: Drifting ion ratios with HP 5970 MSD Organization: Concentric Internet Services Assuming an autotune was performed prior to starting the run. When the mir's fall out of range, go to manual tune and do a profile scan. Are the peak widths of the higher masses significantly different than the autotune? Changing the amu gain/offs can bring them back to autotune. Since you're providing forensic toxicology results, this is not really an option, but merely a clue to the service rep. I have the same problem with a HP 5970. I've tried cleaning the source (baked ceramics too), cleaned the quad., replaced the EM and changed the polarity. Someone suggested that the quad. ceramics were dirty. That's beyond my maintenance skills. Because this problem is most noticeable when the qualifying ions have a significantly different mass than the target ion, I prefer to use this instrument when they a comparable. ****************************************************************************** From: "Lewis K. Pannell" Date: Wed, 04 Feb 1998 12:44:24 EST Subject: Facility Manager, Mass Spectrometry, Laboratory of Neurotoxicology, NIMH Organization: * An opening exists in the Laboratory of Neurotoxicology, NIMH, Bethesda, MD, for a Facility Manager in Mass Spectrometry. Please refer to the following page for information, including the deadline for applications: http://sx102a.niddk.nih.gov/mass/markeyjob.html Replies should be sent to: Dr. S.P. Markey NIH Bldg 10-3D40 Bethesda, MD 20892-1262 fax: 301-480-0198 e-mail: s_markey@codon.nih.gov ****************************************************************************** From: Jeff Lince Date: Wed, 4 Feb 1998 09:30:20 -0800 Subject: RGA for both High and Low pressures Organization: The Aerospace Corporation, El Segundo, CA I need some advice related to our purchase of a new mass spec to be used on a sputter-deposition chamber. We will be using it primarily for process gas analysis when we are sputtering with an argon pressure of ~1-10 mtorr. We are interested in ppm detection (maybe 1-100 ppm) of impurities in the argon, especially water. Also, we'll need to use it for residual gas analysis when at high vacuum prior to sputtering and for helium leak testing. For a number of reasons (e.g., cost <$20K), we've narrowed down the choice to the Leybold Transpector XPR and the Stanford Research Systems CIS. The Leybold is an open ion source RGA with a reduced size quadrapole that takes advantage of the lower mean free path at higher pressures. The SRS is a closed ion source system that requires differential pumping. Any advice/comments on the systems or the companies would be greatly appreciated! --------------------------------------------------------------------- Jeffrey R. Lince office: (310) 336-4464 Research Scientist FAX: (310) 563-3056 Surface Science Department e-mail: jeffrey.r.lince@aero.org The Aerospace Corporation Mail Stop: M2-271 2350 E. El Segundo Blvd. El Segundo, CA 90245 ****************************************************************************** From: Giulio Cozzi Date: Thu, 05 Feb 1998 16:11:08 +0100 Subject: Re: Uranium data production request Organization: Universita' Ca' Foscari Venezia I'm a researcher at Eniromental Sciences Dep. of univerity of Venice, and I'm searching for data about uranium production all over the world. I would be grateful if you could help me about this point, sending to me any kind of available material by e-mail: e.g. html,pdf,Excel Winword files. Thank you so much for your precious help. Best regards Giulio Cozzi p.s. Please reply by e-mail to my address: cozzig@unive.it Giulio Cozzi ++39 41 2578542 Enviromental Sciences Dep. ++39 41 2578549 University of Venice e-mail cozzig@unive.it Calle Larga S. Marta, 2137 I-30123 Venezia Italy @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ ****************************************************************************** From: "bdguest" Date: 5 Feb 1998 21:41:00 GMT Subject: Thanks for the Replies Organization: Insync Internet Services Thanks to all who have replied regarding my inquiry into different benchtop GC/MS systems. There are clearly some strong opinions regarding the different manufacturers, and I appreciate y'all taking the time to send in your opinions. HP has garnered significant support, as well as some well stated criticism. Shimadzu received one good endorsement and a reference to a website that is devoted to Shimadzu's faults. Our quote from Finnigan was out of the ballpark. The other manufacturer's which peaked my interest have been contacted for more info. Thanks again for your help. Any additional opinions will be welcomed. ****************************************************************************** From: cato.brede@kjemi.uio.no (Cato Brede) Date: Fri, 06 Feb 1998 07:54:15 GMT Subject: Re: Need Spectrum Organization: University of Oslo, Norway On 25 Jan 1998 15:15:11 -0500, Fred Rueggeberg wrote: }I am reviewing a manuscript, and would greatly appreciate if someone }could supply me with a Mass Spec of Bisphenol-A. You will find mass spectra at the on-line NIST database: http://webbook.nist.gov/chemistry/ The spectrum of bisphenol-A is found directly at: http://webbook.nist.gov/cgi/cbook.exe?Name=Bisphenol-A&Units=SI&cMS=on Sincerely, Cato Brede _____________________________________ Cato Brede catob@kjemi.uio.no ****************************************************************************** From: Nancy Garland Date: Fri, 06 Feb 1998 12:47:06 -0500 Subject: FTMS book wanted Organization: naval research laboratory I am interested in buying a good copy of the book "Fourier Transform Mass Spectrometry" - ACS Symposium Series #359, 1987. Does anyone have a copy for sale? Thanks. ****************************************************************************** From: "Steven P. Cepa 847-937-7539" Date: Fri, 06 Feb 1998 11:39:13 -0600 (CDT) Subject: MCM-MSDG Offers LC/MS Short Course Organization: * Plans are currently underfoot for the MCM-MSDG to sponsor a one-day short course on "LC/MS". The course will be taught by Robert Voyksner of RTI. Bob has presented this course last to the MCM-MSDG audience in 1993. Back then the transition from thermospray to electrospray was in full force, but thermospray was by no means dead yet. Since then ESI source designs have significantly improved, and I'd be amazed to learn that there were any thermospray sales in the past couple of years. The short course is in the advanced planning stages, so rapid input is needed. Please let us know ASAP how the following details look to you: Location: Hewlett Packard offices in Naperville, IL Date: Tuesday, May 19, 1998 Time: 8:30 to 5:00 Cost: $225/non-members, $200/members Included: snacks and lunch text book (perhaps Richard Cole's "Electrospray Mass Spectrometry" a $90 value) Would you be interested in attending? Do you know of people in your organization who would attend? Please reply to this e-mail (cepas@pprd.abbott.com) or to daniel.l.sweeney@monsanto.com Thank you. ****************************************************************************** From: gal@unice.fr (J.-F. Gal) Date: Sat, 7 Feb 1998 16:15:32 +0000 Subject: spectrum of O2, CO2, N2, He Organization: * Hi, Thomas, The NIST data base contains a lot of mass spectra. For instance CO2 : http://webbook.nist.gov/cgi/cbook.exe?Formula=CO2&Units=SI&cMS=on Good luck, Jean-Francois ------------------------------------- Prof. J.-F. GAL Groupe FT-ICR*GRECFO-Chim. Phys. Org. Fac. des Sciences*Parc Valrose Univ. de Nice-Sophia Antipolis 06108 NICE Cedex 2*FRANCE Tel : (33-4) 04 92 07 61 10 Fax : (33-4) 04 92 07 61 11 E-mail : gal@unice.fr ------------------------------------- ****************************************************************************** From: forenlab@iafrica.com Date: Mon, 09 Feb 1998 16:03:45 +0200 Subject: Guazatine analysis Organization: UUNET Internet Africa Hi everyone. Can anyone help with advice on analysing octamethylenediamine, iminodi(octamethylene)diamine and related compounds. I would like to use LCMS if possible. We have AP-ESI and AP-CI. Have tried ESI but getting too much fragmentation. Sue Peall Forensic Chemistry Laboratory Department of Health Cape Town South Africa forenlab@iafrica.com ****************************************************************************** From: "Paul Martino" Date: Mon, 9 Feb 1998 17:04:13 -0700 Subject: Mass Spectrometer Wanted Organization: Flathead Valley Community College Dear mass.spec members: I am searching for a functional surplused or used mass spectrometer to help teach sophomore organic chemistry. I want to teach using the new discovery based curriculum that is now being developed. Most discovery based experiments require sophisticated analytical instrumentation. (Most experiments incorporate GCMS.) Any help in locating a mass spectrometer system would be appreciated. FVCC has a small equipment budget and limited space, so expensive systems, or sector instruments are not feasible. Thank you very much. Paul Martino Please reply by email at pmartino@fvcc.cc.mt.us or call (406) 756-3895. *********************************************** * Dr. Paul Martino * * Flathead Valley Community College * Chemistry Department * 777 Grandview Dr. Kalispell, MT 59901 * * Phone: 406-756-3895 FAX: 406-756-3815 * * Email Address: pmartino@fvcc.cc.mt.us * *********************************************** ****************************************************************************** From: Mark Atlas Date: Tue, 10 Feb 1998 16:27:11 GMT Subject: Recent listings on Going, GoingSold!!! online lab equipment auction Organization: Netcom Recent listings on Going, Going…Sold!!! online lab equipment auction The Internet's only true auction and backed by auctioneers in the California State Auctioneers Assoc. has recently listed many new items for bid. Come by and see our growing and extensive virtual inventory. Our guide to buying used lab equipment is also available Recent listings Bids open Beckman 6300 Amino Acid Analyzer $35,000 Gilson HPLC $5,000 Waters 996 PDA detector $ 4,750 Beckman DU 70 Spec $4,500 Beckman DU 7 Spec $3,000 Waters 717 Autosampler $4,750 Waters HPLC system (present models) $15,500 Waters GPC system $11,000 More equipment is being consigned every day. These are great values at 20-45 cents on the dollar . Many items are refurbished, There are over 40 items presently on the site. and bids are occurring weekly. http://www.going-going-sold.com ****************************************************************************** From: reilly@sec.med.upenn.edu (G B Reilly) Date: 11 Feb 1998 17:21:38 GMT Subject: Standard for 20KD-60KD Organization: University of Pennsylvania In using a Voyager TOF MALDI there are supplied methods files for up to 20KD and above 60KD. What have people used as standards for peptides between 20KD and 60KD? ****************************************************************************** From: X97DERSNAH@wmich.edu Date: Wed, 11 Feb 1998 15:48:24 -0500 (EST) Subject: Mass Spectrometry Question Organization: * I was reffered to this address by a sight at Emory U. I have to give a presentation on a MS manufacturer named Associated Instruments Corp but I cannot find any info on the web. Has this company mergered with another manufacturer, been bought out or ceased operations? I would appreciate any info you could provide. Thanks. A. Dersnah ****************************************************************************** From: foxsteve Date: Thu, 12 Feb 1998 00:18:59 -0800 Subject: Re: Mass Spectrometry Question Organization: Netcom X97DERSNAH@wmich.edu wrote: } } I was reffered to this address by a sight at Emory U. I have to give a } presentation on a MS manufacturer named Associated Instruments Corp but I } cannot find any info on the web. Has this company mergered with another } manufacturer, been bought out or ceased operations? I would appreciate any } info you could provide. Thanks. } } A. Dersnah Dear Mr. Dersnah: Maybe it help you "Cases on Manufacturing and Operations Management from Harvard Business School Publishing Pub # Title Author 689052 Associated Instruments Corp.: Analytic Instruments Division, Wheelwright SC, Dugal S" Steve Ivkov ****************************************************************************** From: Gunter Kuhnle Date: Thu, 12 Feb 1998 09:27:31 +0100 Subject: Carotenoids with MALDI or ESI Organization: Uni Leipzig I would like to get mass spectra of carotenoids with MALDI or ESI. Unfortunately, there is no much information in literature about this. Does anyone has experience with this and could give me some information (solvent, matrix, etc)? Thanks, Gunter Kuhnle ****************************************************************************** From: gt6801a@prism.gatech.edu (Wilson Z. Shou) Date: 12 Feb 1998 09:36:46 -0500 Subject: Re: Carotenoids with MALDI or ESI Organization: Georgia Institute of Technology I remember seeing a review paper on Anal. Chem. about using LC/MS (including stop-flow FAB, particle beam, ESI and APCI) to analyze carotenoids. I'm not sure about the exact volume and page number, but it should be around late 95 to 96 and the paper was in the cover of that issue. I suspect the ability of MALDI to do this though. Hope this helps. ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: Carotenoids with MALDI or ESI Date: Thu, 12 Feb 1998 15:18:23 GMT Organization: JEOL USA, Inc. In <6busbo$lk0@acmez.gatech.edu> Gunter Kuhnle writes: }I would like to get mass spectra of carotenoids with }MALDI or ESI. Unfortunately, there is no much information }in literature about this. }Does anyone has experience with this and could give me }some information (solvent, matrix, etc)? }Thanks, }Gunter Kuhnle I haven't tried MALDI or ESI for carotenoids, but the absence of polar or basic functional groups and the relatively low molecular weight suggests that you might do better to use APCI instead of ESI. Also, these compounds are soluble in non-polar solvent such as benzene and chloroform, which are not favored solvents for ESI. You might see if acetonitrile will work. -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: Armel Le Bail Date: Thu, 12 Feb 1998 22:53:23 +0100 Subject: ARRED (Academic Research Rare Expert Directory) Organization: Private ARRED is a new 'Academic Research Rare Expert Directory' conceived for facilitating contacts between those seeking for an expert and the experts expecting such contacts. http://www.cybercable.tm.fr/~cristal/welcome.html Thanks for considering your own addition, and feel free to make suggestions. Armel Le Bail - Universite du Maine - Laboratoire des Fluorures Avenue O. Messiaen - 72085 Le Mans cedex 9 - France Web: http://fluo.univ-lemans.fr:8001/ ****************************************************************************** From: mccomb@cc.UManitoba.CA (Mark McComb) Date: 13 Feb 1998 20:15:06 GMT Subject: CE-ESI-MS Organization: The University of Manitoba Hello all, I am just about to interface a home made CE with a Micromass QQQ using a sheathless ESI source. I am using external power supplies, 0-30 kV for CE and 0-5 kV for ESI. Does anyone have experience with this type of setup? Are there any precautions to take. Any tips? Any information is much appreciated. Mark. -- ================================================================== = Mark E. McComb, B.Sc.,M.Sc. Department of Chemistry = = phone (204) 474-9321 University of Manitoba = = fax (204) 474-7608 Wpg. MB. R3T 2N2 = = CANADA = = The more that you read, the more things you will know. = = The more that you learn, the more places you'll go. Dr.S. = ================================================================== ****************************************************************************** From: Jens Griep-Raming Subject: Finnigan MAT 212 accessories Date: Fri, 13 Feb 1998 13:32:31 +0100 Organization: Universitaet Oldenburg [This is forwarded from sci.chem.labware. DB] Anybody out there, using a Varian (Finnigan) MAT 212 with a DEC Datasystem PDP 11? We have some accessories for sale (we´d rather prefer an exchange into some boxes with german beer) The accessoriers are: - RAM extension 256kB each (2 pc) - Data-Cartridges DC-600 for backup (approx. 30 pc) - Lark-Cartridges (7 pc) - Ribbon for Printronix-MVP-printer (4 pc) - Printronix MVP printer field maintenance manual - Ribbon for DEC-printer LA-100 (4 pc) - Ribbon for DEC-printer LA-36 (3 pc) - Documentation for DEC PDP11 (some manuals, many 5¼"-disks) - printer-paper for the printronix-printer 2000 sheets 12"x375mm (2pcks) Anybody who is interestet, should mail me. Jens -- ********************************** Jens Griep-Raming Universität Oldenburg FB 9 Chemie AG Metzger / Massenspektrometrie Postfach 2503 26111 Oldenburg Germany griep@chemie.uni-oldenburg.de Tel. +49 441-798-3731 ********************************** ****************************************************************************** From: "Zhang L." Date: Fri, 13 Feb 1998 19:44:14 -0600 Subject: Chemistry Site Organization: Washington University in St. Louis Chemistry WWW links by Henry Li-Kang Zhang at: http://artsci.wustl.edu/~lkzhang/chemistry.html Not the best, but worth to have a look. Thanks. Henry L. Zhang ****************************************************************************** From: gal@unice.fr (J.-F. Gal) Date: Sat, 14 Feb 1998 10:56:55 +0000 Subject: Carotenoids with MALDI or ESI Organization: * Hi Gunther, You wrote "I would like to get mass spectra of carotenoids with MALDI or ESI. Unfortunately, there is no much information in literature about this." There is a recent review in Anal Chem, 1996, 68, 299A-304A, "Carotenoid analysis using LC/MS" by R.B. van Breemen, with a few references to ESI (the cover is very colorful). The same author has written on APCI of carotenoids : JMS, 1996, 31, 975-981. You may check with the author (Univ of Chicago, College of Pharmacy) if he knows about MALDI works. Best wishes. ------------------------------------- Prof. J.-F. GAL Groupe FT-ICR*GRECFO-Chim. Phys. Org. Fac. des Sciences*Parc Valrose Univ. de Nice-Sophia Antipolis 06108 NICE Cedex 2*FRANCE Tel : (33-4) 04 92 07 61 10 Fax : (33-4) 04 92 07 61 11 E-mail : gal@unice.fr ------------------------------------- ****************************************************************************** From: wah45@aol.com (WAH45) Date: Sat, 14 Feb 1998 06:53:26 -0500 Subject: need a method for quantitating polypropylene glycol in water. Organization: AOL http://www.aol.com I need a method to quantitate ppm of PPG (polypropylene glycol) mw unknown in water. I've tried a few different techniques but am having problems. Does anyone have a method. ****************************************************************************** From: V Bajic Date: 14 Feb 1998 11:58:09 GMT Subject: CFP: Int. SSCC98 Conf., 22-24 Sep. 1998, Durban, South Africa (long) Organization: University of Natal Conference info UPDATE: 23 January 1998 ___________________________________________________ CALL FOR PAPERS INTERNATIONAL CONFERENCE ON SYSTEMS, SIGNALS, CONTROL, COMPUTERS (SSCC'98) 22-24 September 1998, Durban, SOUTH AFRICA __________________________________________________ ORGANIZED BY International Association for the Advancement of Methods for System Analysis and Design (IAAMSAD) and Academy of Nonlinear Sciences as a part of YEAST 1998 activity in South Africa ________________________________________________________________ HOST ORGANIZATION: Centre for Engineering Research (CER) of Technikon Natal, Durban South Africa ________________________________________________________________ SUPPORTING ORGANIZATIONS: SAICSIT - South African Institute for Computer Scientists and Information Technologists IEEE South Africa Section SAIMC - South African Institute of Measurement and Control SANBI - South African National Institute for Bioinformatics M L Sultan Technikon (South Africa) CER - Centre for Engineering Research, Technikon Natal (South Africa) ________________________________________________________________ HONORARY CHAIRMAN: Academician V.M.Matrosov (Russia) CONFERENCE CHAIRMAN: V.B.Bajic (South Africa) ________________________________________________________________ ADVISORY BOARD: V.B.Bajic (South Africa), J.Brzobohaty (Czech Republic), P.Daoutidis (USA), W.Hide (South Africa), C.Morabito (Italy), V.V.Kozlov (Russia), P.Leach (South Africa), P.C.Müller (Germany), L.Shaikhet (Ukraine), E.Rogers (UK), H.Szu (USA) ________________________________________________________________ INTERNATIONAL PROGRAMME COMMITTEE: A.Al-Dhelaan (Saudi Arabia), V.Apanasovich (Belarus), V.B.Bajic (South Africa), C.Berger-Vachon (France), J.Brzobohaty (Czech Republic), M.Campolo (Italy), P.Daoutidis (USA), T.Fukuda (Japan), Z.Gajic (USA), M.Gams (Slovenia), J.Gil Aluja (Spain), L.T.Gruyitch (France), H.Hahn (Germany), M.Hajek (South Africa), R.Harley (South Africa), W.Hide (South Africa), M.Jamshidi (USA), V.Kecman (New Zealand), B.Kovacevic (Yugoslavia), V.Krasnoproshin (Belarus), V.V.Kozlov (Russia), P.Leach (South Africa), L.K.Kuzmina (Russia), V.Milutinovic (Yugoslavia), C.Morabito (Italy), P.C.Müller (Germany), H.Nijmeijer (The Netherlands), D.H.Owens (UK), D.Petkov (South Africa), K.M.Przyluski (Poland), E.S.Pyatnitskii (Russia), E.Rogers (UK), L.Shaikhet (Ukraine), A.V.Savkin (Australia), H.Szu (USA), E.I.Verriest (USA), R.Vrba (Czech Republic), J.Ziska (Czech Republic) ________________________________________________________________ LOCAL ORGANIZING COMMITTEE: V.B. Bajic, P.Govender, R.Hacking, M.Hajek, M.McLeod, K.S.Moodley, R.Papa, C.Radhakishun, A.Singh ________________________________________________________________ PLENARY SPEAKERS: T.Fukuda (Japan) L.T.Gruyitch (France) R.Harley (South Africa) M.Jamshidi (USA) V.M.Matrosov (Russia) P.C.Müller (Germany) D.H.Owens (UK) H.Szu (USA) ___________________________________________________________________ GENERAL INFORMATION ON YEAST 1998 1998 is the year of Science and Technology in South Africa (YEAST 1998). The intention of the Department of Arts, Culture, Science and Technology of SA is to make South Africans more aware of how Science and Technology affects them in every-day life. Such a national initiative is a very good environment for a conference like this: one that has a broad scope and spans many different fields. At the same time an opportunity is given to the research community of South Africa to interact more directly with overseas peers. ___________________________________________________________________ AIMS AND SCOPE OF SSCC98 CONFERENCE The Conference is broad in scope and will provide a forum for the exchange of the latest research results as applied to different branches of science and technology. The areas of interest include concepts, techniques and paradigms associated with systems, signals, control and/or computers. Domains of application include informatics, bio- medical technology, economics, management, diverse engineering and science fields and applied mathematics. Artificial intelligence techniques are of particular interest, as well as reports on industrial applications. The authors are requested to include up to five codes from the list provided (or relevant keywords) that are most appropriate to the topics covered in their papers. A detailed list of topic codes necessary for classification of submissions is provided in this message. The conference will include several plenary and invited lectures from world renowned scientists, regular papers and posters. A number of special and invited sessions will also be organized, dealing with focused areas of interest. The proposals for these special sessions should be submitted at the same time as the abstracts. A special session cannot have less than three papers or more than six. The official language of the conference is English. __________________________________________________________________ MANUSCRIPT SUBMISSION - REVIEW PROCESS Three copies of the extended abstract having about two pages, should be sent to the Conference Office at the address provided. Alternatively they may be faxed (one copy). Full papers are preferred. Abstracts (papers) in Microsoft Word or in LATEX format (with standard LATEX style for articles) can be sent by e-mail. All submissions will be reviewed by members of the International Programme Committee; additional reviewers will be consulted if necessary. The submissions will be reviewed as soon as they arrive; the average review time is about four weeks. Authors of accepted papers will thereafter be informed (by e-mail if available) of the required format for camera-ready paper submissions. In order for reviewers to be able to assess the submissions, the extended abstract has to provide sufficient information about the background to the problem, the novelty of the obtained results and the results achieved, the conclusions drawn and some references. Up to five keywords (or topic codes from the list provided in this message) should be supplied. All submitted papers have to be original, unpublished and not submitted for publication elsewhere. ___________________________________________________________________ PROCEEDINGS All accepted papers will be published in the Conference Proceedings. ___________________________________________________________________ IMPORTANT NOTICE Although we expect that the authors of accepted papers will present their papers at this Conference, we recognize that circumstances may prevent authors from participation at the Conference. In such cases the accepted papers will be published if the authors inform organizers of their non-attendance at the Conference by 15th May 1998. However, conference fees according to established rules have to be pre-paid in order that papers appear in the Proceedings. ___________________________________________________________________ CONFERENCE FEES The conference fee for one participant covers the publication of paper(s) (each with a maximum of five A4 pages in length) according to the required format; one volume of the Proceedings in which the paper(s) appear(s); refreshment during the conference and a banquet. Additional volumes of the Proceedings can be purchased for US$ 55.00. A social programme and tourist visits will be provided at extra cost. Members of SAIMC are entitled to 10% conference fee discount. Reduced registration fee of US$ 250.00 (South Africans R 1000.00) is applicable for early received, reviewed and accepted papers provided this fee is paid by the end of February, 1998 - prospective authors are encouraged to take advantage of this convenience; otherwise the following rates apply: Early registration fee: US$ 300.00 (South Africans R 1200.00) Late and on-site registration fee: US$ 350.00 (South Africans R 1400.00) Student fee: US$ 200.00 (South Africans R 800.00). The student scale of fees applies when all authors mentioned on the paper are current students; written proof has to be provided at the time of payment. Payment in South African rands is possible only when all authors of the papers are South African residents; written proof has to be provided at the time of payment. ___________________________________________________________________ DEADLINES Extended Abstracts and Special Session Proposals: - submission by mail or fax (10th April, 1998) - submissions by e-mail (10th April, 1998) Notification of acceptance (25th April, 1998) Submission of papers in camera ready form (15th May, 1998) Early payment of conference fees (15th May, 1998) Late payment of conference fees (end of June, 1998) ___________________________________________________________________ CONFERENCE VENUE Library complex, Technikon Natal, Berea Campus, Durban ___________________________________________________________________ ADDRESS OF THE CONFERENCE OFFICE: SACAN, P.O.Box 1428, Link Hills 3652, South Africa Tel.: (+27 31) 204-2560, (+27 31) 204-2736 Fax: (+27 31) 204-2560, (+27 31) 204-2063 e-mail: sscc98@umfolozi.ntech.ac.za alternative e-mail: bajic.v@umfolozi.ntech.ac.za __________________________________________________________________ CONFERENCE WEB HOME PAGE http://nsys.ntech.ac.za/iaamsad/SSCC98.html __________________________________________________________________ TOPIC CODES The authors are requested to include those codes (which are provided below; or the relevant keywords) that are most appropriate to the topics covered in their papers. Up to five codes are allowed. For example, codes A5, AI7, M12 indicate that the closest description of the paper topics will be associated with: A5: Economics, Management, Banking, Finance AI7: Knowledge acquisition M12: Mathematical Programming, Optimization, Operations Research ____________________________________________________ ____________________________________________________ Some of the topics include, but are not limited to: ____________________________________________ BM Bioinformatics and Biomedical Engineering ____________________________________________ BM1: Biocybernetics, Biocomputing BM2: Medical Informatics BM3: Bioinformatics, Signal Processing in Genetics, Genetic Databases BM4 : Biological Effects of EM Radiation, Medical Applications EM Radiation BM5 : Hearing Aids, Prosthetics __________________________ AI Artificial Intelligence __________________________ AI1 : Artificial Intelligence Theory, Tools and Technologies AI2 : Possibility theory, Fuzzy Sets and Fuzzy Systems AI3 : Neural Networks, Computational Intelligence, Neural-Fuzzy Systems AI4 : Learning and Adaptation Algorithms and Methods AI5 : Expert Systems, Knowledge-based Systems AI6 : Genetic Algorithms, Evolutionary Systems AI7 : Knowledge Acquisition AI8 : Benchmark Problems ________________________________________________ M System Modelling, Simulation, Analysis, Design ________________________________________________ M1 : Multimedia, Animation M2 : Bond-Graphs, Petri Nets, Scheduling M3 : Mathematical and Computer Modelling M4 : Expert Dystems for Modeling, Fuzzy and Neural Network Modelling M5 : Model Reduction, Model Validity M6 : Identification, Data Acquisition, Fault Detection, Sensors M7 : Large Scale and Complex Systems, Discrete Event and Hybrid Systems, Stochastic Systems M8 : Linear/Nonlinear Systems, Uncertain Systems M9 : Distributed Parameter Systems, Time-Delayed Systems M10 : Algorithms, Parallel and Distributed Simulation, Simulation Tools M11 : CAD, FEM M12 : Mathematical Programming, Optimization, Operations Research M13 : Decision Theory, Decision Support Systems M14 : Risk Assessment and Management, Qualitative Reasoning ___________ SIG Signals ___________ SIG1 : DSP, Waveltes, Fast Algorithms SIG2 : Spectra Estimation, FFT and Spectral Analysis SIG3 : Higher Order Spectral Analysis SIG4 : Multidimensional Signal Processing, Morphological Signal Processing SIG5 : Filters, Filtering, Adaptive Filtering SIG6 : VLSI for Signal Processing, Array Signal Processing _________ C Control _________ C1 : Advanced and Conventional Control C2 : Controllability and Observability C3 : Intelligent Control (Fuzzy, Neural Networks, Genetic Algorithms) C4 : Mechatronics, Man/Machine systems, Real-time Control C5 : Optimal Control, Predictive Control, Robust Control, Stochastic Control C6 : Stability, Qualitative Analysis, Sliding Mode Control C7 : Monitoring and Supervision Systems _____________ COM Computers _____________ COM1 : Architectures COM2 : Algorithms, Programming Languages and Technologies COM3 : Distributed and Parallel Processing COM4 : Data Compression COM5 : Man-Computer Interaction, Virtual Reality COM6 : Image Processing and Computer Vision COM7 : Networks, Internet, WWW Tools _____________________________________________ SC Scientific Computing, Applied Mathematics, Numerical Methods _____________________________________________ SC1 : Computational Linear Algebra SC2 : Symbolic Computation, Neural Computing SC3 : Nonlinear Science SC4 : Methods for ODE's and PDE's SC5 : Integral Equations SC6 : Parallel Algorithms and Computing SC7 : Computational Physics/Chemistry/Biology/Acoustics SC8 : Graph theory, Discrete Mathematics ___________________ A Application Areas ___________________ A1 : Manufacturing, Robotics, Industrial Engineering A2 : Control, Process Industry A3 : Power and Energy Systems, Electrical Engineering, Instrumentation A4 : Communication, Multimedia, Rural Telecommunications A5 : Economics, Management, Banking, Finance A6 : Information Systems, Computer Systems A7 : Environmental Systems, Pollution, Safety Engineering A8 : Medicine, Biology, Bioengineering, Genetics A9 : Decision Making, Operations Research, Policy Making A10 : Transportation, Aviation A11 : Water Resources A12 : Chemistry A13 : Mining, Geology, Rock Dynamics A14 : Vibration, Structures, Materials A15 : Fluid Dynamics, Thermodynamics A16 : Social Sciences A17 : Education, Training Technology, Technology Transfer A18 : Other (describe) relevant applications __________________________________________________________ PRELIMINARY REGISTRATION In order to provide you with the up-to-date information, we ask you to make a preliminary registration for the conference. This preliminary registration IS NOT binding - it is to help us to organize the conference better. The preliminary registration form can be accessed from the conference Home Page or directly at http://nsys.ntech.ac.za/iaamsad/SSCC98reg.htm (this form of preliminary registration is the preferred one); alternatively, send us the information required by e-mail or fax, using the form provided below SSCC'98 BASIC INFORMATION (FAX OR E-MAIL) _______________________________________________ First Name(s): _______________________________________________ Surname: _______________________________________________ Title: _______________________________________________ Position: _______________________________________________ Institution: _______________________________________________ Address: _______________________________________________ City: _______________________________________________ Zip: _______________________________________________ Country: _______________________________________________ Tel.: _______________________________________________ Fax: _______________________________________________ e-mail: _______________________________________________ Web Home Page: _______________________________________________ I intend to submit the paper(s) - Title of paper(s): _______________________________________________ I am interested in organizing a special session (title of the session): _______________________________________________ I intend to participate at the conference (Yes/No): _______________________________________________ Mail conference information also to (give address or e-mail address): _______________________________________________ ******************************************************** SSCC98 Conference Secretariat Tel. (+27 31) 204 2560 Fax (+27 31) 204 2560 Email: sscc98@umfolozi.ntech.ac.za Alternative Email: bajic.v@umfolozi.ntech.ac.za WWW HomePage: http://nsys.ntech.ac.za/iaamsad/SSCC98.html ******************************************************** ****************************************************************************** From: aabi@earthlink.net Date: Sun, 15 Feb 1998 18:32:59 -0800 (PST) Subject: Gel Documentation & Analysis Organization: * Greetings. Please enter "Remove" to remove from this list. We offer 20% lower pricing. View & download Windows & Mac programs: 1D, 2D, DNA, RFLP, blots, ELISA, Colony counting, Particle sizing, Chromosomes Karyotyping, & image analysis. We offer gel documentation, chemiluminescence, densitometers, & color Image analysis ****************************************************************************** From: "rat" Date: Mon, 16 Feb 1998 15:08:13 +1100 Subject: Re: Carotenoids with MALDI or ESI Organization: Monash Uni Hi Gunter, I've run some carotenoids in APCI, works quite well, tried the same with ESI with less success. Stuart Thomson Facility manager - Mass Spectrometry Victorian College of Pharmacy Monash University Parkville Victoria Australia http://www.vcp.monash.edu.au/chemistry/mspec.html Gunter Kuhnle wrote in message <6busbo$lk0@acmez.gatech.edu>... }I would like to get mass spectra of carotenoids with }MALDI or ESI. Unfortunately, there is no much information }in literature about this. } }Does anyone has experience with this and could give me }some information (solvent, matrix, etc)? } }Thanks, } }Gunter Kuhnle } } ****************************************************************************** From: Werner Spahl Date: Mon, 16 Feb 1998 13:35:09 +0100 Subject: References for negative ESI-HR? Organization: [posted via] Leibniz-Rechenzentrum, Muenchen (Germany) Hi, I'm looking for reference substanzes for negative ESI-HR. Chapman (Practical Organic Mass Spectrometry) mentiones PEG or PPG Sulfates but I can't find them in any chemical catalog. Where can I buy them or can they be made out of the appropriate PEGs or PPGs in a simple way? Werner Spahl (ui22273@sun1.lrz-muenchen.de) "The meaning of my life is to make me crazy!" ****************************************************************************** From: CHS292@news.ac.uk (J) Date: Mon, 16 Feb 98 17:55:39 GMT Subject: amu Organization: U Is this defined in terms of the mass of the atom 12C or the ion 12C+ ? J ****************************************************************************** From: Gunter Kuhnle Date: Mon, 16 Feb 1998 15:36:27 +0100 Subject: Electrospray -- Ionization --- Mechanism? Organization: Uni Leipzig Hallo, could anyone recommend a recent review on the mechanism of ESI? I know the paper by Malcolm Dole assuming a Rayleigh-Coulomb "explosion", and the theory of John Fenn and others, it is a field desorption. The most recent paper I have is from the beginning of the 90s - and there is no final description on the mechanism but only an "it has to be explored" statement. Thanks in adavance, Gunter Kuhnle -- ************************************* Gunter Kuhnle Universitaet Leipzig/Institut fuer Biochemie Talstrasse 33 D-04103 Leipzig/Germany kuhnle@rzaix340.rz.uni-leipzig.de FAX: #49-341-97-36998 ************************************* ****************************************************************************** From: "Ravindra H.Aglave" Date: Mon, 16 Feb 98 10:08:18 CST Subject: used turbomolecular pump Organization: * Hello I am looking for a used turbomolecular pump to replace a now defunct Balzers TPU 050 with a 63CF. IF anyone has any information on such a pump or a similar which is to be sold/given away, please forward it to me. It can come as integral part of some vacuum system or by itself. This pump will run a Dycor quadrupole MS system for gas-mixture analysis. -- Thank you. __________________________________________________________________ Ravindra H.Aglave ravi@cer.ce.utulsa.edu U340 KEH (Combustion Research Group) Dept. of Chem Engg. Off: (918) 631-2980 University of Tulsa Res: (918) 583-0568 600 S.College Ave. fax: (918) 631-3268 Tulsa Ok 74104 ___________________________________________________________________ ****************************************************************************** From: l.p.brull@chem.ruu.nl (lars brüll) Date: Mon, 16 Feb 1998 17:25:03 +0200 Subject: Re: Electrospray -- Ionization --- Mechanism? Organization: Nose Job Inc. In article <6c9lf0$i16@acmey.gatech.edu>, Gunter Kuhnle wrote: } Hallo, } } could anyone recommend a recent review on } the mechanism of ESI? Gaskell S.J., Electrospray: Principles and Practise, Journal of Mass Spectrometry, 32, 677-688 (1997) Lars Brüll _________________________________________ _________________________________________ Utrecht University Faculty of Chemistry Bijvoet Center for Biomolecular Research Department of Mass Spectrometry Sorbonnelaan 16 3584 CA, Utrecht The Netherlands. Phone +31 30 2537992 Fax +31 30 2518219 http://www.chem.ruu.nl/amsmass/www/amsmass.html _________________________________________ _________________________________________ ****************************************************************************** From: "Pedro Domingues" Date: Mon, 16 Feb 1998 17:11:50 +0100 Subject: Re: References for negative ESI-HR? Organization: Universidade de Aveiro, Portugal Werner Spahl wrote in message <6c9dnq$qh8@acmez.gatech.edu>... }Hi, I'm looking for reference substanzes for negative ESI-HR. Chapman }(Practical Organic Mass Spectrometry) mentiones PEG or PPG Sulfates but }I can't find them in any chemical catalog. Where can I buy them or can }they be made out of the appropriate PEGs or PPGs in a simple way? Hi You can find several PEG's (Polyethylene glycol) on Sigma or BDH catalog. Al you have to do is choose one that has a average molecular wt. near the mass of your substance. You should have great care with contamination's: - Use the small concentrations of PEG (less than 50 ng/microliter) - Reserve seringes and other material for PEG - Beware with problems due to the contamination of the source, loops etc. We usually aquire first the sample spectra and, only after, do the calibration Hope this helps Pedro Domingues Chemistry Dpt. Aveiro University Portugal ****************************************************************************** From: David Sparkman Date: Mon, 16 Feb 1998 15:52:57 -0500 Subject: ESI Mechanism Articles Organization: * Gunter Kuhnle wrote asking for current information on the mechanism of the electrospray process. There are several good articles on the ESI process in Richard Cloe's (ed.) "Electrospray Ionization Mass Spectrometry, Fundamentals, Instrumentation, and Applications", Wiley, 1997 (ISBN 0-471-14564-5). Chris Enke has proposed an explaination of the ESI processes in Anal. Chem. 69(23), 4885-4893, 1997. "A Predictive Model for Matrix and Analyte in Electrospray Ionization of Singly-Charge Ionic Analytes". John Fenn, Joan Rosell, and Chin Kai Meng had an article in the November 1997 issue of JASMS, p1147. This also appeared in JASMS Online Aug. 7, 1997. "In Electrospray Ionization, How Much Pull an Ion Need To Escape Its Droplet Prison". O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: "Salane" Date: Mon, 16 Feb 1998 15:15:12 -0600 Subject: Re: need a method for quantitating polypropylene glycol in water. Organization: Arkansas Children's Hospital and University of Arkansas for Medical Sciences (UAMS) Easy, but expensive, ion spray LC-MS WAH45 wrote in message <6c53tj$gg7@acmey.gatech.edu>... }I need a method to quantitate ppm of PPG (polypropylene glycol) mw unknown in }water. I've tried a few different techniques but am having problems. Does }anyone have a method. } } ****************************************************************************** From: D.Selby@unsw.edu.au (David Selby) Date: Tue, 17 Feb 1998 08:47:04 +1000 Subject: Re: Electrospray -- Ionization --- Mechanism? Organization: UNSW In article <6c9lf0$i16@acmey.gatech.edu>, Gunter Kuhnle wrote: } could anyone recommend a recent review on } the mechanism of ESI? I know the paper by } Malcolm Dole assuming a Rayleigh-Coulomb } "explosion", and the theory of John Fenn and } others, it is a field desorption. } } The most recent paper I have is from the beginning of } the 90s - and there is no final description on the mechanism } but only an "it has to be explored" statement. } Gunter, Kebarle and Tang wrote a detailed paper on the mechanism of electrospray mass spectrometry in 1993: P.Kebarle and L.Tang, Analytical Chemistry (1993), vol 65, 972A-986A I hope this helps David Selby -- David Selby School of Chemistry University of New South Wales D.Selby@unsw.edu.au ****************************************************************************** From: David Sparkman Date: Mon, 16 Feb 1998 19:39:47 -0500 Subject: re: amu Organization: * Someone wrote: }Is this defined in terms of the mass of the atom 12C or the ion 12C+ ? amu is an obsolete symbol for atomic mass. It has nothing to do with ions which are expressed in mass-to-charge ratio units. The following are the facts. amu: According to the IUPAC Guidelines on Mass Spectrometry Nomenclature, amu is no longer recommended as the symbol for mass. This was the symbol for a unit of mass when the standard was based on "O 16". Physicists reported mass based on the most abundant naturally occurring isotope of oxygen (16O – established by Francis William Aston [1877-1945] in 1929 after his discovery that oxygen was composed of three different isotopes, two of which had a higher mass [17O and 18O] than the most abundant isotope) being assigned an exact mass of 16 (1 amu = 1/16 of the mass of 16O). This was the basis of the physical atomic mass scale. Chemists used amu to define a unit of mass as 1/16 the atomic weight of oxygen (officially established in 1905 on the suggestion of the Belgium chemist Jean Servais Stas [1813-1891]). This was the basis for the chemical atomic mass scale. The two scales differed by a factor of 1.000275 (physical > chemical). An atomic weight is the weighted average of the naturally occurring stable isotopes of an element, and oxygen has three. The atomic weight of oxygen was an absolute value of 16, by definition. This made the determination of the atomic weight of newly discovered elements easy by forming their oxides. To eliminate this ambiguity, the standard of a single mass unit being 1/12 the most abundant naturally occurring stable isotope of carbon (^12C) was adopted in 1961. The symbol for the unified atomic mass unit was established as u—NOT µ nor amu. Prior to the oxygen standards, the bases for atomic mass had been set around 1805 by John Dalton (1766-1844) as 1 for the lightest element, hydrogen. In 1815, the Swedish scientist Jöns Jacob Berzelius (1779-1848) set the atomic weight (relative atomic mass) of oxygen to 100 in his table of atomic masses; however, this was not widely accepted. Stas’s recommendation of setting the "oxygen 16" standard allowed hydrogen to retain a mass close to 1, thereby keeping Dalton’s scale somewhat intact. Inappropriate: A.M.U. or a.m.u.: This can be used as an abbreviation for atomic mass unit. However, it should be avoided because of the confusion with the symbol amu (see amu above). The symbol for atomic mass unit is u. Note: The use of amu, a.m.u., or A.M.U. as a label for the ordinate of a mass spectrum is incorrect. All of these terms refer to "mass" and the ordinate of the mass spectrum represents values of mass-to-charge ratios of ions, not the "mass" of ions. Therefore, it is just as improper to label the ordinate of the mass spectrum dalton, Da, u, or mass. In older literature, the ordinate of the mass spectrum was often labeled "mass". This was because in nearly every case the number of charges on the ion was one. After the development of the electrospray interface and the analysis of biomacromolecules with the presence of multiple-charge ions became widespread, the use of "mass" on the ordinate of the mass spectrum (while always incorrect) became more inaccurate and problematic. Care must also be taken in the use of the terms molecular weight and molecular mass. The molecular weight of a molecule is based on the atomic weights of its atoms, whereas the molecular mass is based on either the nominal or monoisotopic masses of its elements. u: This is the symbol for a mass unit or the unified atomic mass unit. It represents 1/12th the mass of the most abundant naturally occurring stable isotope of carbon. The term dalton (symbol Da) is used in biochemistry and is accepted in mass spectrometry; however, it has not been approved by the Conférence Générale des Poids et Mesures. The mass of a given particle is the sum of the atomic masses (in daltons) of all the atoms of the elements composing it. The use of the symbol u is referenced in Quantities, Units and Symbols in Physical Chemistry, Blackwell, 1988. O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: "A.P.Bruins" Date: Tue, 17 Feb 1998 10:35:34 CET Subject: Re: CE-ESI-MS Organization: Pharmacy Dept Groningen University >Mark McComb (mccomb@cc.UManitoba.CA) wrote: > >Hello all, >I am just about to interface a home made CE with a Micromass QQQ using a >sheathless ESI source. I am using external power supplies, 0-30 kV for >CE and 0-5 kV for ESI. Does anyone have experience with this type of >setup? Are there any precautions to take. Any tips? Any information is >much appreciated. >Mark. > >-- >================================================================== >= Mark E. McComb, B.Sc.,M.Sc. Department of Chemistry = >= phone (204) 474-9321 University of Manitoba = >= fax (204) 474-7608 Wpg. MB. R3T 2N2 = >= CANADA = >= The more that you read, the more things you will know. = >= The more that you learn, the more places you'll go. Dr.S. = Answer: You must be careful in the electrical connection of your column to the 5 kV power supply. The CE current will flow through this supply, so that it may have to sink too much current and be damaged. See the schematic and discussion on page 112 in my chapter of the book on Electrospray edited by Richard Cole. Andries Bruins Dr. A.P. Bruins University Centre for Pharmacy A. Deusinglaan 1 9713 AV Groningen, The Netherlands phone +31-50-363-3262 fax +31-50-363-3311 a.p.bruins@farm.rug.nl ****************************************************************************** From: audi@csn-hp.in2p3.fr (Georges AUDI) Date: 17 Feb 1998 11:26:09 GMT Subject: Re: amu Organization: CSNSM Orsay On 16 Feb 1998 08:08:18 -0500, J wrote: }Is this defined in terms of the mass of the atom 12C or the ion 12C+ ? The atomic mass unit (amu) is by definition 1/12th of the mass of the carbon 12 atom. To know more, check the ATOMIC MASS DATA CENTER (AMDC) at http://csnwww.in2p3.fr/amdc/ Best regards, Georges Audi ------------------------------------------------------------ C.S.N.S.M. (IN2P3-CNRS) courriel: audi@csnsm.in2p3.fr Batiment 108 www: http://csnwww.in2p3.fr/ 91405 Orsay Campus (France) ****************************************************************************** From: CH292@news.ac.uk (J) Date: Tue, 17 Feb 98 18:05:16 GMT Subject: re: amu Organization: U Thank you for your deatiled reply. My reason for asking the question was that m/z for 12C+ could, in principle, be determined by mass spectrometry whereas m for 12C couldn't. J ****************************************************************************** From: "Richard B. van Breemen" Subject: Re: Carotenoids with MALDI or ESI Organization: * > >I would like to get mass spectra of carotenoids with >MALDI or ESI. Unfortunately, there is no much information >in literature about this. > >Does anyone has experience with this and could give me >some information (solvent, matrix, etc)? > >Thanks, > >Gunter Kuhnle > Please read my review articles or original research papers on electrospray LC-MS of carotenoids as follows: R. B. van Breemen, "Liquid Chromatography/Mass Spectrometry of Carotenoids." (1997) Pure & Appl. Chem., 69, 2061-2066. R. B. van Breemen, "Innovations in Carotenoid Analysis Using LC/MS." Anal. Chem., (1996) 68, 299A-304A. R. B. van Breemen, "Electrospray LC-MS of Carotenoids." Anal. Chem., (1995) 67, 2994-2009. or APCI LC-MS of Carotenoids: R. B. van Breemen, C. R. Huang, Y. Tan, L. C. Sander, A. B. Schilling, "Liquid Chromatography/Mass Spectrometry of Carotenoids Using Atmospheric Pressure Chemical Ionization." J. Mass Spectrom., (1996) 31, 975-981. or these papers on MALDI of carotenoids: R. Kaufman, T. Wingerath, D. Kirsch, W. Stahl, H. Sies, "Analysis of Carotenoids and Carotenol Fatty Acid Esters by Matrix-Assisted Laser Desorption Ionization (MALDI) and MALDI-post source decay Mass Spectrometry." Anal. Biochem. (1996) 238, 117-128. T. Wingerath, W. Stahl, D. Kirsch, R. Kaufmann, H. Sies, "Fruit Juice Carotenol Fatty Acid Esters and Carotenoids as Identified by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry." J. Agric. Food Chem. (1996) 44, 2006-2013. There really is plenty in the literature concerning mass spectrometry of carotenoids. Richard B. van Breemen Department of Medicinal Chemistry & Pharmacognosy University of Illinois at Chicago College of Pharmacy 833 S. Wood Street Chicago, IL 60612-7231 ****************************************************************************** From: Eric_Block@waters.com Date: Tue, 17 Feb 1998 10:13:51 -0400 Subject: reply to Gunter Kuhnle Organization: * Gunter Kuhnle Wrote: >I would like to get mass spectra of carotenoids with >MALDI or ESI. Unfortunately, there is no much information >in literature about this. > >Does anyone has experience with this and could give me >some information (solvent, matrix, etc)? > >Thanks, > I have run carotenoids by particle-beam interface-if you're not sample-limited (i.e. you can put a few ug on-column) you get very nice library-searchable EI spectra. I have also done some work with retinol degradants. As Dr. Cody has correctly surmised, APCI (positive) worked best, even though the mobile phase was 100 organic phase. ****************************************************************************** From: "Kevin P. Grady" Subject: WTB: Fisons/VG Trio 1 or Trio 1000 Date: Mon, 16 Feb 1998 18:30:00 -0500 Organization: The DataXchange Network, Inc [This is forwarded from sci.techniques.spectroscopy. DB] Wanted Fisons Trio1 or Trio 1000 parts or complete systems. Contact by email elfana@aol.com or Kevinpg@excaliber.net ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: References for negative ESI-HR? Date: Tue, 17 Feb 1998 15:12:05 GMT Organization: JEOL USA, Inc. In <6c9dnq$qh8@acmez.gatech.edu> Werner Spahl writes: }Hi, I'm looking for reference substanzes for negative ESI-HR. Chapman }(Practical Organic Mass Spectrometry) mentiones PEG or PPG Sulfates but }I can't find them in any chemical catalog. Where can I buy them or can }they be made out of the appropriate PEGs or PPGs in a simple way? } Werner Spahl (ui22273@sun1.lrz-muenchen.de) }"The meaning of my life is to make me crazy!" We used PEG sulfates for negative-ion high-resolution ESI measurements (Cody et. al. Anal. Chem. 1992, 64, 1561-1569), following the report by Mark Hemling of the use of these compounds for negative-ion FAB. The synthesis of these compounds was reported in Mark's ASMS abstract See Hemling, M. "Negative Ion FABMS Reference Compounds", Proc. of the 38th ASMS Conference on Mass Spectrometry and Allied Topics, Tucson, AZ, June 3-8, 1990. Or...you can obtain some Ivory dishwashing soap. That is a mixture of polyether sulfates. Use the old, milky white liquid and not the new clear liquid. You will see reference masses from about 200 u to up to at least 1000 u. I don't believe that I've posted the masses on our web page, but I'll try to do that some time in the near future. Good luck! -- "For purposes of ... New Jersey Right to Know Act. Contents partially unknown." |____________ |_ Robert B. Cody, Ph.D |________________________________ Product Development Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ http://www.jeol.com |__________ e-mail: cody@nojunkmail.jeol.com |_ (REMOVE 'nojunkmail' TO REPLY) ============== Do not send me spam or advertising via e-mail !! ========= ****************************************************************************** From: "Dave White" Date: 17 Feb 1998 20:38:27 GMT Subject: Re: Fisons/VG Trio 1 or Trio 1000 Organization: Micron Internet Services We have a differentially pumped EI/CI Trio-1, with data system, GC and all software, for $20,000. There is also an optional probe and autosampler - price negotiable. Dave White Research Scientific Services, Inc. spectrac@micron.net www.rsservices.com Kevin P. Grady wro