****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 1 Jan 1997 02:57:28 GMT Subject: Re: rat poison Organization: AOL http://www.aol.com The inverted chromatograms are often seen when the instrument is scanned from a relatively low starting value. Although it depends upon the solvent system/ionization mode the following are a few ideas. In ESI/APCI if you scan low there will be the inevitible chemical background detectable under ALL conditions. This background is both chemical species in the form of "contaminants" and cluster ions forming and reforming in the source. The great softness of atmospheric sources that every one loves also allows the operation of these under conditions in which cluster formation is more or less favored. A headache for some and plain old chemistry to others. Basically the input of the desolvation energy that removes the LC solvent from the analyte can also fragment the analyte if applied in too large an amount. Conversely you get clusters of LC solvent and analyte if you run it it too low. You also get clusters of LC solvent without analyte. Like three methanols, a wate,r and a proton. These are frequently observed as a series of ions with a statisticall bell curve like distribution with a typical spacing value of say water or methanol. Now under some conditions delicate labile analytes may require lower energy inputs to avoid fragmentation. These same conditions may favor cluster formation at low mass. So what to do? ANS: Scan from a mass Higher than the inevitable background ions. Usually these sources are being used because the analysis won't work by EI/CI with a GC (smaller molecules are usually more volatile and /or less labile). The inverted chromatograms can occur when the desired analyte elutes off the column in to the busy source (as explained above) and begins to compete for ionization. If the source contains a plasma like APCI then the plasma cycle components and clusters grabbing up the charges will begin to donate some to (or be robbed by) the analyte. If you are scanning low enough you are actually monitoring the depletion of these species (and so the deplelted chromatogram (observeable in GC/MS using CI as well) If you know the mass of your charged analyte then the plotting out of this value should give you a nice single ion plot that you can use to quantitate your species. Even when the RIC or TIC looks bad. The background ions may cahnage over a gradient as the solvent compostion cahnges. So you might consider that as well before you decide what starting m/z value you are going to use for your analysis. ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 1 Jan 1997 03:02:26 GMT Subject: Re: rat poison Organization: AOL http://www.aol.com Sorry I forgot to sign that previous message on Rat poison and inverted chromatograms. The nice thing is that you don't usually fragment the molecules with these sources so ther is really not much reason to look down low. Of course there are exceptions. ps A frequent error is the failure to differentiate polymer background cont. and cluster formation when something is not right with the source (equipment or operator error). Matt Sweeney Mass-Spec Gun for hire ****************************************************************************** From: Christopher Kaine Date: Tue, 31 Dec 1996 23:35:54 -0800 Subject: Thanks Organization: OneNet Communications HUB News Server Hello Finnigan Customers: I am writing to say thanks for allowing me to be part of your world. My tenure as one of Finnigan's Technical Specialists/Institute Instructors has come to an involuntary conclusion. It has been my honor and pleasure to assist some of you in the use and maintenance of your instrumentation for the past eight years. I plan on frequenting this newsgroup to continue volunteering my expertise/experience. Again, I thank you all. Cordially; Christopher J. Kaine ****************************************************************************** From: gbird@superlink.net (George ) Date: Wed, 01 Jan 1997 14:34:57 GMT Subject: Re: Software to interpret GC-MS spectra from first principles Organization: SuperNet Inc. (908) 828-8988 On 31 Dec 1996 09:16:55 -0500, cody@jeol.com (Chip Cody) wrote: }Some research has gone into predicting fragmentation based on a known }structure -- I dabbled with this myself and I saw a really nicely done }version of a program that actually draws reaction mechanisms presented }by someone from NIST at a recent ASMS meeting. However, I understand that }this program is still in the development stages. } Chip; I was unable to attend the ASMS this year, if you hear any other information about this program, could you let me know? thanks.... ****************************************************************************** From: Terry Hulbert Date: Thu, 02 Jan 1997 12:26:15 -0800 Subject: Physics Express Letters Organization: Institute of Physics Publishing Institute of Physics Publishing has launched the latest version (v2.0) of Physics Express Letters (PEL). PEL now offers FREE access to all letters and rapid communications from 12 of our journals. Journals within the service include Journal of Physics B, Journal of Physics: Condensed Matter, Classical & Quantum Gravity, Measurement & Science Technology and Semiconductor Science & Technology. You can find Physics Express Letters at Terry Hulbert Producer, Electronic Products Institute of Physics Publishing ****************************************************************************** From: pvang@sci.kun.nl (Peter van Galen) Date: 6 Jan 1997 09:15:53 +0100 Subject: Re: Software to interpret GC-MS spectra from first principles Organization: University of Nijmegen, The Netherlands In <5agb24$en0@acmey.gatech.edu> gbird@superlink.net (George ) writes: }On 31 Dec 1996 09:16:55 -0500, cody@jeol.com (Chip Cody) wrote: }}Some research has gone into predicting fragmentation based on a known }}structure -- I dabbled with this myself and I saw a really nicely done }}version of a program that actually draws reaction mechanisms presented }}by someone from NIST at a recent ASMS meeting. However, I understand that }}this program is still in the development stages. }} }Chip; } I was unable to attend the ASMS this year, if you hear any other }information about this program, could you let me know? }thanks.... Please attach me as another interested person. thanks in advance |___________________________ Peter M. van Galen, |____ Mass Spectrometry |__________________ Organic Chemistry Department |_ Nijmegen University |___ Toernooiveld 1, 6525 ED Nijmegen,Netherlands ****************************************************************************** From: George Shpenkov Date: Mon, 06 Jan 1997 14:11:14 +0200 Subject: New Theory of Matter-Space-Time (long article) Organization: Technical University in Bydgoszcz For : Scientists and students with an interest in the physics, mathematics, chemistry, philosophy, logic, interdisciplinary sciences. ------------------------------------------------------------------------ ALTERNATIVE PICTURE OF THE WORLD by LEONID G. KREIDIK & GEORGE P. SHPENKOV "...The book is for those who think the physical theories, far from ending, have only just begun..." Published by GEORGE SHPENKOV Bydgoszcz, Poland _____________________________________________________________________ Physics, Chemistry, Mathematics, Philosophy, Logic, Interdisciplinary Sciences ------------------------------------------------------------------------ Alternative Picture of the World By L.G. Kreidik, Polytechnic Academy, Minsk, Belarus G.P. Shpenkov, Technical University, Bydgoszcz, Poland Dialectics of Nature, considered primarily in this book, is an integrated physical-mathematical science which represents, in the broad sence of the word, a logical-philosophical system of knowledge about Nature. The authors have concentrated on general problems of physical axioms, which are considered within the dialectics, including logic, philosophy and mathematics. The wave three-dimensional material-ideal numerical field and dialectical logic of the same structure constitute the basis of the dialectics. The book consists of three volumes: Volume 1. 1. Mathematical expression of the main categories of philosophy and logic. 2. Kinematics and dynamics of exchange. Volume 2. 3. Structure of Space of the Universe 4. Electrostatic and electromagnetic fields. 5. Particles and exchange in an electormagnetic field. Volume 3. 6. Atomic structure of matter-space-time and physical properties of substance. 7. Physics and philosophy. Contents of the second and third volumes is based on the notions described in the first one. VOLUME 1. As is known, the main characteristic feature of dialectics is a juncture of the material and ideal Worlds, which is the most important contradiction of the Universe. This contradiction joins all the other contradictions of the Universe. Of all philosophic systems, dialectics alone expresses the objective contradictions most completely by considering them as a basis for everything that exists in the Universe. Translating the concept of contradictions and noncontradictions into the language of mathematics, the authors have constructed a mathematical description based on the mathematics of the material-ideal numerical field, which allows sufficiently accurate expression of the laws of dialectics in mathematical form and, proceeding from them, a description of axioms of physics, and mathematics. The language of the main notions and axioms of physics and mathematics is extended substantially, the theory of discrete derivatives and the quantitative-qualitative version of operational calculus are presented, and notions of a multiplicative derivative and multiplicative integral are introduced. Against to classical tradition, motion and rest are concerned in close nonseparable contradictory relation. This dialectical bond are expressed by a dialectical material-ideal field of numbers and a binary set of notions. Every binary pair of the notions describes corresponding aspects of motion and rest giving opportunity to see both kinetic and potential sides of kinematics of real processes. Description of dynamics has also dialectical binary character. The nonforce description of relations between objects, called as "exchange", differs in principle from the classical approach based on the notion of force interaction. Exchange, in wide sence of this word, is here the exchange by motion, rest, space, matter, arbitrary state, etc. VOLUME 2. A new approach based on solution of the wave equation in the material-ideal qualitative-quantitative space was used for description of the internal nucleonic structure of atoms. The relation of the internal geometry of nucleonic nodes of atoms with the crystal shape was found. In the present theory, the electrostatic field is an ultra frequency field, which brings about new physical constants. The theory of electric charges is presented and a charge formula is obtained and used to calculate the electronic charge. The theory of mass is expounded and used to obtain formulae for electron and nucleon masses. This will allow new relations between the basic physical constants to be established, in particular, the mass of the electron and its charge. On the basis of the wave theory, new calculation formulae are given for some mass and energy exchange coefficients. In particular, the theory of Planck constant is considered and its formula is presented. All constants and variables are expressed in a system of units that does not contain fractional powers in the formulae of dimensions. VOLUME 3. It is shown that classical physical phenomena of transfer (diffusion, viscosity, thermal conductivity) are naturally described in terms of the suggested nucleonic model of atoms. It represents a system of shells with discrete points-nodes of wave space completed by nucleons, i.e. an atom of the space has not a customary nucleous of a classical atomic model. Moreover, a nucleon of the atom has the megaspace apart from microspace. Gravitational shells of the nucleon correlates with corresponding shells of star systems. Other processes, such as the motion of nucleons and of nucleon excitations (electrons) in solids (electric resistance, Hall ffect), absorption, radiation, scattering energy, etc. confirm the correctness of the presented model of an atomic space as well. The nucleon structure of atoms is considered in their agreement with periodic table as a sequential series of the periodicly recurring and nonrecurring solutions of the wave equation. Nonlinear phenomena are dealt with in the volume. The central place occupies the consideration of Doppler effect, which is closely related to the theory of relativity and Mössbauer effect, etc. New QUGCS system of units forms a significant part of the volume. Some dialectical aspects of the Universe, main postulates of dialectical philosophy and logic, elements of the theory of sets and concrete dialectics are touched upon here briefly. Copyright © 1996 by Leonid G. Kreidik & George P. Shpenkov ISBN 83-906156-0-6 (Vol. 1, 158 pages, soft cover) ISBN 83-906156-1-4 (Vol. 2, 164 pages, soft cover) ISBN 83-906156-2-2 (Vol. 3, 186 pages, soft cover) Publication: November-December 1996 Price: US$ 150 (per three volumes together) ------------------------------------------------------------------------ ORDER FORM Send this Order to: George Shpenkov, Stroma 13A/402, Bydgoszcz, 85-158 Poland Rush Orders: FAX 48-52-438643 Please send me______copy(ies) of_______________________________________ ________________________________________________________________________ All orders must be pre-paid (publisher pays postage) Payment to: PBK SA w Warszawie. O/Gdansk-F/Bydgoszcz, ul. Chodkiewicza 15, Bydgoszcz, 85-197 Poland 370800-62095-2700-2-1-787-1 Name __________________________________________________ Address ________________________________________________________________ ________________________________________________________________________ Signature__________________________ Date_____________________________ Dr. LEONID G. KREIDIK has long been working at the Polytechnic Academy in Minsk as a lecturer. He teaches physics. His scientific interest, past and present, deals mainly with the analysis of the groundwork of physics on the basis of world achievements in Philosophy, Logic, system analysis and modern computer technologies. His fanatic faithfulness to the fundamental science ever since his graduation from the Physics Department in 1955 led him to the mathematically strict solutions of general physics fundamentals not published before and generalized in this book. His ideas has not been coincided to the official philosophy at that time. He has predicted, many years ago, possibilities of the nuclear catastrophes paying attention to the famous scientists at the nuclear technology defects. He has announced about it, before the Chernobyl disaster, in the Center of Nuclear Research in Dubna to Academician George Fliorov and Nobel prize winner Igor Tamm in 1970. Dr. GEORGE P. SHPENKOV is currently a Professor of the Institute of Mathematics and Physics at the Technical University in Bydgoszcz, Poland. He graduated from the Radiophysics Department in Minsk, 1965, and then worked at the Physical-Technical Institute name after Ioffe (St. Petersburg). He defended his Ph.D. there in the Physics of Semiconductors. He discovered laser radiation in A2B4C52 semiconductor compounds and generation of the second harmonic in them. He has D.Sc. degree in Solid State Physics; he also worked at the Radiation Introscopy Laboratory and at the Substance Structure Department of the University in Minsk. University lecture classes he has taught include all sections of General Physics, Quantum Mechanics and Quantum Chemistry, Contemporary Physics (for philosophers), etc. He is well known in science (tribology) as the author of three books, the latest of which is "FRICTION SURFACE PHENOMENA" (ELSEVIER, Amsterdam, 1995, 358 pages). He is one of the discoverers of the "HYDROGEN WEAR OF METALS" phenomenon. He is also the author of 16 inventions and approximately 90 publications. His professional education and experience in science and teaching Physics is wide enough to allow him to deeply understand the problems of general physical rules, to have doubts about the central concepts of Modern Physics and to attempt to dare their complete analysis. Subjects of current interest include an analysis of basic concepts of Physics and their co-ordination with Logic and Philosophy. ISBN 83-906156-0-6 (Vol. 1) ISBN 83-906156-1-4 (Vol. 2) ISBN 83-906156-2-2 (Vol. 3) ****************************************************************************** From: cogsol@barnes.globalnet.co.uk (Cognitive Solutions Limited) Date: Mon, 06 Jan 1997 16:31:16 GMT Subject: Free Training Software Demos Organization: * Try our website for free demos of training Software for Analytical Techniques. http://www.globalnet.co.uk/~cogsol ****************************************************************************** From: STROHJ@pfizer.com Date: Mon, 6 Jan 1997 14:28:38 -0500 Subject: Free mass spectrometer Organization: * From: Justin G. Stroh, Pfizer Inc. We are looking to find a good home for a BioIon mass spectrometer, which is in good working order. The instrument is free for the asking with two caveats. First, the person(s) receiving the instrument must pick it up at Pfizer's research facility in Groton, CT. The second is that the person(s) picking up the instrument must have a valid NRC license to use californium-252. If you know of such a person, please have them contact me directly at strohj@pfizer.com. Thank you for your help in this matter. Justin G. Stroh strohj@pfizer.com ****************************************************************************** From: tectra@t-online.de (Andreas Gati) Date: 7 Jan 1997 21:11:02 GMT Subject: ion source / duoplasmatron needed Organization: tectra GmbH Physikalische Instrumente Hi all, can anyone help me finding an ion source / duoplamatron source with the following specs: - mass seperation: H, AR (or Ne) - energy range 500 eV to 10 keV (at least 5) currents at 500 eV: H > 10 uA, Ar > 100 uA - spot size 2 mm dia Any help appreciated with thanks Andreas Gati ****************************************************************************** From: eri@mindlink.bc.ca (Elemental Research Inc.) Date: Tue, 7 Jan 1997 21:57:00 GMT Subject: Parts for VG PQ-I ICPMS for sale Organization: MIND LINK! - British Columbia, Canada As I stated here a couple of weeks ago, we have some spare parts for an old VG Plasmaquad-I ICP-MS for sale. We have sold the bulk of them (as the result of posting to this newsgroup) but some still remain. Last chance to pick up these hard to find items. Price List (all prices in $US) A. Working Units ------------- Lens supply (s/n 1894-05) $1480.00 Lens supply (s/n 1993-03) $1480.00 Power control (s/n 3001) $ 740.00 RF coupler $ 370.00 B. Main power board PCB's: ----------------------- MR504-200C3 issue 2 (1) $ 148.00 each MR504-204C3 issue 3 (1) $ 148.00 each MR504-207C3 issue 1 (2) $ 148.00 each MR504-202C2 issue 5 (2) $ 148.00 each MR504-205C3 issue 3 (2) $ 148.00 each MR504-201C3 issue 2 (4) $ 148.00 each MR504-203C3 issue 2 (2) $ 148.00 each MR504-2013C3 issue 1 (2) $ 148.00 each C. Quad Controller Boards ------------------------- MA 3020-200 P/D (1) $ 148.00 MA 495-69C (1) $ 148.00 MA 495-70C (1) $ 148.00 MA 495-63C (1) $ 148.00 MA 495-65C (1) $ 148.00 MA 495-76C (1) $ 148.00 MA 485-62C (1) $ 148.00 D. Misc. --------------- Edwards vacuum controller model H1105 (1) $2000.00 Quad RF generator, valve type (2) $2000.00 Best regards, Ray Ford Elemental Research North Vancouver, B.C. Tel: (604) 986-0445 Fax: (604) 986-0071 Email: eri@mindlink.bc.ca ****************************************************************************** From: Ed Nieves Date: Thu, 9 Jan 1997 12:32:15 -0500 Subject: Position open: Research Associate Organization: * Position open: Research Associate Salary $25-40,000 per year, depending on experience. The project involves isolating novel peptides that may be involved with a variety of biological processes, including obesity, aggression, anxiety, and many others. The peptides are isolated from mouse tissues by purification on affinity columns and HPLC, or by 2D gels. Sequence analysis is then performed by tandem MS and/or Edman degradation. Experience with peptide isolation and/or mass spectrometry is desirable. For further details, contact Lloyd Fricker (fricker@aecom.yu.edu) Dept. of Molecular Pharmacology Albert Einstein College of Medicine. ****************************************************************************** From: kosse@pioneer.state.nd.us (Kevin J. Kosse) Date: Fri, 10 Jan 1997 16:16:35 GMT Subject: Borneol and related compounds Organization: U S WEST - !NTERACT Services Does anyone have a method, or a reference to a method, for borneol, iso-borneol, or methyl-iso-borneol in water at part-per-trillion levels? TIA Kevin J. Kosse ****************************************************************************** From: "G. Wolmershaeuser" Subject: MS Simulation Program Date: Fri, 10 Jan 1997 11:18:36 +0100 Organization: University of Kaiserslautern [This is forwarded from sci.chemistry.analytical. Dr. Wolmershaeuser has found two programs that simulate mass spectra, but needs something more talented and powerful. DB] I am looking for a good and versatile simulation program for mass spectra. PD or shareware for UNIX or WINDOWS(NT or 95) platorm preferred! Any idea? Thanks a lot in advance -- G. Wolmershaeuser FB Chemie, Univ. Kaiserslautern wolmersh@chemie.uni-kl.de ****************************************************************************** From: Eric Nelson Date: Fri, 10 Jan 1997 13:35:09 -0500 Subject: Does anyone know a good RRKM program?? Organization: Purdue University I'm looking for a program to calculate RRKM statistics for mass spec reactions. I find several on the web that give you the rate from the theromchemical parameters, but I'd like to go the other way and get the reaction barrier from the rate of reaction (which we can measure). Does anyone know of such a program?? ****************************************************************************** From: lippy@UVic.CA (D.Lippert) Date: Sat, 11 Jan 1997 22:23:24 GMT Subject: 204 contaminant Organization: UVic Micro-Sequencing Center I have a question concerning a possible contaminant in a sample analyzed by ESI. We know that the sample contains a biologically active peptide. However, ESI of this material shows no peptide peaks, instead we see a series of peaks which are all multiples of 204 (204, 408, 613, and 817). CID data of the larger peaks shows that they all collapse to 204, and the 204 peak fragments to give 187, 169, 158, and 145. Does anyone recognize this pattern? Could it be a membrane lipid or a plasticizer? If we knew what it was we could try removing it with chromatography, but as it stands, we can only guess as to what techniques may work. Thanks in advance for any answers. Dustin ****************************************************************************** From: Valentin <@chemistry.gatech.edu,@nac.no:Shironosov@ikar.udm.ru> Date: Sun, 12 Jan 1997 19:40:25 +0400 Subject: NEW resonance technologies and LEVITATION.(very long article) Organization: Scientific-Research Center "IKAR" NEW resonance technologies and LEVITATION. Shironosov V.G I. Levitation... it is very simple! (popular) II. New resonance technologies. III. Resonant confinement of bodies and particles (from elementary up to macro) in inhomogeneous electromagnetic fields without any external feedback. (for the specialists) I. LEVITATION... IT IS VERY SIMPLE! Such, indeed , is the case in science. First we think that it cannot be! Then... yes, it is possible. Finally we come to a conclusion: it is obvious, it is simple. The temple of charm ( a mechanical, optical and physical study of the well-known Russian illusions creator Gamuletsky which has existed up to 1942 ) was famous for its staircase. Going upstairs the visitors could see from the very below a gold angel's figure levitated in the air without any support. It was as big as a human being. When coming up you could see Seraphim lifting a French horn to his lips and playing it. His fingers moved as if he was a real person. According to Gamuletsky's notes 10 years had been spent on searching of the 'supporting' point i.e. for the location and weight of the magnet for the figure to be kept in the air. Most likely Gamuletsky felt that there were deep inner relations of the world with the cut-and-try method, errors and calculations. He made a break-down into the unknown. But further recommendations for the wonder to be made had not been left. In their turn Orthodox Moslems believe that a coffin with the Prophet's body is hanging in the air without any support. As Euler wrote it was said that Mahomet's coffin was being supported by the force of the magnet (p.159, (1) ). Is it possible or not? Perelman considers it to be impossible. If thanks to the magnetic attraction the similar equilibrium was observed even for a moment the slightest blow of the wind would be enough it - and then the coffin would fall down on the floor or would be tightened to the ceiling. To keep it motionless is practically impossible. It can be compared with a cone standing on its top. Though from the theoretical point of view it is quite acceptable (p.159, (2) ). Different reports on levitation of the subjects in the localized area are often perceived as something pseudo-scientific. Eventually the phenomenon was called - levitation. The encyclopedia explains it as " a rising of the body up to the air without any mechanism". But there is no scientific explanation to this phenomenon yet. Probably it is impossible to do this on the basis of the concepts and approaches that exist in physics. But we should not be satisfied with the ancient equivalent which explains levitation as " a supernatural ability to become light whenever we need , at our will ". The concept mixes everything up: legends, myths, and all surprising facts. Actually, everything is much easier. There is no prophet in Motherland. As early as last century Peter Lebedev, Russian scientist, described resonance as the most characteristic phenomenon in the nature. In engineering and machinery resonance was diligently bypassed. Until now in the school textbooks an example on resonance (a bridge has fallen down because of the soldiers platoon marching , though the military instructions say that bridges should be passed along in a free step ) is given in the very ordinary way. Resonance can be explained in different ways. Two events took place at one and the same time: A team of the foreign scientists of three persons in 1989 was rewarded with the Nobel Prize for the non-resonant levitation of the charged particles in the electronic traps without feedback. Resonance levitation continued to consider impossible. A team of the Russian scientists of three persons has proved theoretically and experimentally (for macro-body) the possibility of resonant confinement of bodies and particles (from elementary up to macro ) in inhomogeneous electromagnetic fields without any external feedback ( Letters to JTP, JPT, RAS USSR, 1983). The idea itself was suggested as early as in 1972 (Filatov A.I.). As it had happened many times in our country the work of the scientists has not been noticed. From both theoretical and experimental points of view a break-down has been made and the bases for the new technologies have been laid. The possibility of the resonant selective hanging up of the bodies and particles in the inhomogeneous electromagnetic fields without any external feedback has been demonstrated. For the non-professionals: such small subjects as "pens and pencils" were levitating without any support inside the installation in the course of the experiment. The foundations for the new resonance technologies have been laid by Lebedev (1890) and those experiments. It was the basis for the great improvement of the economy. As a result in 1989 that research 'was rewarded'. The scientific subdivision was excluded from the Academy of Sciences as if "it was not working effectively". A bridge between the science and the budget was broken. A great improvement turned out to be a defeat. A flight appeared to be a fall. It is very difficult to survive, but we must not betray our Motherland. It is a sin. The first ready to fly will be the winner. For the resonance tech need to have the wings - this is the reason why the Scientific-Research Center "IKAR" was born. The international information system on resonance technologies with the electron carriers will serve to reach the aim. "MIS - RT" is supposed to be produced in SRC "IKAR" in Izhevsk. Resonance was called " the most characteristic state for the nature by Lebedev, a russian scientist. According to the latest data it is a very stable state, probably, the most one. History is a witness to this: as long as a man had not tried to interfere in the earth's processes there were no disasters on Earth. By now a man has been lost in serious diseases. Our body consists of the synchronous frame of the ordered resonant - interacting molecules. Each cell has a clock which strikes the time. In case if one or another cell dies or does not react correctly the whole body will start suffering. Sometimes it leads to death. The very same processes take place in a human society. It looks as if in Russia a chain reaction has started its work. Tens of streams used resonance technologies are pulsing in many regions of the country: laser; resonance mechanisms, tools, flying machines, conveyers considering their high quality technical parameters they differ from those we have now; new systems of activation of liquid medium which allow consumption of energy to be much reduced - e.g. when purifying water to have it free from chlorine, dioxine or when intensifying the technological processes in agriculture , food production, building construction, e so on; unique high effective installations with low energy and non-contact influence where the resonance effect is used to treat and prevent different kinds of diseases. II. New resonance technologies By now it is possible to recognize the most promising directions in the area of resonance technologies: -development of resonant traps for the particles of different types and size (from elementary up to macro) without an external feedback; investigation of properties and dynamics of the certain particles in the traps (including electrons, atoms and molecules with their further packing on a board - this is a molecular technology); development of resonant traps where plasma is to be used; self - confinement plasma; -development of the new non-contact methods for prophylactics, treatment and diagnostics on the basis of effect of resonant fields on biological systems; biochemical reaction control; -selective separation of different kinds of powders (magnetic, ferromagnetic, abrasive etc.) for magnetic information carriers (magnetic disks, tapes) in particular; -new resonant supersensitive sensors of the fields (electromagnetic, acoustic, hydrodynamic, seismic, gravitational); -robotization i.e. spatial non-contact orientation, confinement and controlling of microparts when assembling systems, instruments and devices; -weighing, confinement and movement of bodies (engine rotors, toys, transport where a magnetic suspension is used). III. Resonant confinement of bodies and particles (from elementary up to macro ) in inhomogeneous electromagnetic fields without any external feedback. P.S. (for the specialists): Impossibility of levitation is usually based on the strict statement (Braunbec) that it is impossible to build up a stable configuration using non-conducting charged or magnetized bodies (Irnshow's theorem) in a medium with dielectric permittivity or magnetic permeability higher or equal to 1. It occurs due to the following fact: there are the only special points so called 'saddle points' for the potential energy of any body in the static field (electrical, magnetic, gravitational...) with no sources of the field in the area of weighing i.e. there are no stable 'pits' where a body can be settled. In one direction the body will correspondingly be involved into the area of weighing and in the other one it will be pushed out. But what is forbidden in statics may turn out to be allowed in dynamics ( in the variable fields when the bodies are moving themselves ). Perhaps the first statement of this could have been seen in Mathieu's work (1838 ) devoted to the problem of the diaphragm vibration. It turned out to be that in the corresponding equation (Mathieu's one): .. X +(h+2e*cos(2*Z))*X=0 (1) where X = dX / dz , z = w * t, w is frequency of vibration ,a dynamic stability of the unstable state in statics ( h<0 ) out of the resonance area had simultaneously been assumed and described. An example of this is a dynamic stability of the inverted pendulum with a vibrating point of a support described by ( 1 ) at small angles of its deviation from a vertical. Simple reasonings show that at large w vibration of the point of the support of the pendulum is equivalent to the appearance of the effective returning force to a vertical. When the rod of the pendulum is accelerated downwards the angle X decreases by X1 accordingly a deviating moment of the forces decreases when the rod keeps on moving up. As a consequence, the angle ( X-X1 ) will increase only by X2 Date: Mon, 13 Jan 1997 12:32:17 +1000 Subject: Re: 204 contaminant Organization: Monash University D.Lippert wrote: } } I have a question concerning a possible contaminant in a sample } analyzed by ESI. We know that the sample contains a biologically } active peptide. However, ESI of this material shows no peptide peaks, } instead we see a series of peaks which are all multiples of 204 (204, } 408, 613, and 817). CID data of the larger peaks shows that they all } collapse to 204, and the 204 peak fragments to give 187, 169, 158, and } 145. Does anyone recognize this pattern? Could it be a membrane } lipid or a plasticizer? If we knew what it was we could try removing } it with chromatography, but as it stands, we can only guess as to what } techniques may work. Thanks in advance for any answers. } } Dustin Dear Dustin, Can you give us some idea of the conditions that you are measuring under? Also what id the nature of the sample, is the peptide synthetically produced or isolated etc? Their is a fair chance it is a buffer salt of some sort. Cheers Stuart Thomson. ****************************************************************************** From: Jo Rita Jordan <76150.2171@CompuServe.COM> Date: 13 Jan 1997 14:52:10 GMT Subject: Shimadzu web site scam Organization: Analytical Consumer Analytical Chemists: There appears to be a fake message on an anonymous Web site -- and on the search engines! -- about Shimadzu and Kratos. It could be from a competitor or from a disgruntled user. Pay no attention to anonymous information! I also got two anonymous letters pointing out the site -- one may have been sent out by one of those spamming services. This is a new way the Web can be dangerous. Anonymous libel. Be on your toes! Pass the word. Jo Rita Jordan, Editor and Publisher Analytical Consumer ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: MS Simulation Program Organization: JEOL USA, Inc. Date: Mon, 13 Jan 1997 17:32:30 GMT In <5b5r1r$3d3@acmex.gatech.edu> "G. Wolmershaeuser" writes: }[This is forwarded from sci.chemistry.analytical. Dr. Wolmershaeuser has }found two programs that simulate mass spectra, but needs something more }talented and powerful. DB] }I am looking for a good and versatile simulation program for mass }spectra. PD or shareware for UNIX or WINDOWS(NT or 95) platorm }preferred! Any idea? }Thanks a lot in advance }-- }G. Wolmershaeuser }FB Chemie, Univ. Kaiserslautern }wolmersh@chemie.uni-kl.de What kind of simulation are you looking for ? If the intention is to calculate fragments from a known structure, see my reply to Ian Laubscher in this news group ("Software to Interpret Mass Spectrum from First Principles"). Are you looking for teaching software, or research software ? I would suggest Kermit Murray's MS page as the best central location for MS resources on the Internet: http://tswww.cc.emory.edu/~kmurray/mslist.html Good luck with your search, and please let us know what programs you find most helpful! -- ========================================================================= |____________ |_ Robert B. Cody, Ph.D |________________________________ Applications Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ |__________ http://www.jeol.com |_ e-mail: cody@jeol.com ========================================================================= ****************************************************************************** From: Iris Klaiber Date: Mon, 13 Jan 1997 22:25:26 +0100 Subject: 3-Hydroxy-palmiticacid Organization: CC University of Hohenheim (not responsible for contents) We are searching for 3-Hydroxy-palmiticacid (or another 3-Hydroxy-fatty- acid) as reference component to determine the configuration of our isolated one, which we have only in a very small amount. Does anyone have a method, or a reference to a method, for the determination of the configuration (R or S) with an maximum of 0.3 mg? Thanks in advance for any answers. Iris _______________________________________________________________ Iris Klaiber email: klaib130@uni-hohenheim.de University of Hohenheim Tel.: ++49-711-459-2248 Dep. for Chemistry Fax.: ++49-711-459-2951 Garbenstr. 30 70599 Stuttgart Germany ______________________________________________________________ ****************************************************************************** From: eri@mindlink.bc.ca (Elemental Research Inc.) Date: Tue, 14 Jan 1997 19:07:10 GMT Subject: Environmental chemistry position available at ICP-MS lab Organization: MIND LINK! - British Columbia, Canada Elemental Research Inc. of North Vancouver, British Columbia is a research/environmental/materials science laboratory specialising primarily in ICP mass spectrometry. We have a job opening for a project chemist trainee in the environmental chemistry section of the laboratory. The new person would start out in an entry level position, receive training in the instrumentation and lab procedures used at ERI, and progress to a project chemist position where he/she would be responsible for all the data for particular clients/projects. Applicants should have some experience in mass spectrometry, preferably ICP mass spectrometry, and in environmental chemistry; e.g. in ion chromatography and standard water parameters. Salary would depend on qualifications and experience. Elemental Research Inc. is one of the most respected ICP mass spectrometry labs in the world. It is located in a desirable suburb of Vancouver, Canada, which is one of the most beautiful and sought after cities in the world in which to live. Fax or email resumes to: David Gray Lab Manager Elemental Research 309-267 West Esplanade North Vancouver, B.C. Canada V7M 1A5 Tel: (604) 986-0445 Fax: (604) 986-0071 Email: eri@mindlink.bc.ca Web site: www.eri-icpms.com ****************************************************************************** From: Christopher Kaine Date: Tue, 14 Jan 1997 20:25:00 -0800 Subject: Thank-you Finngan Customers Organization: OneNet Communications HUB News Server Finngan Customers: It has been my pleasure to assist and train some of you during my 8 year tenure with Finnigan. It is Finnigan management's plan that my and other educated/trained and experienced mass-spectroscopist Technical Specialist's services are no longer required for the customers or Service Engineers. I will though, continue to avail myself to this newsgroup to offer what ever expertise I may. Again, I thank-you and wish you all the best in the coming years. Cordially; Christopher J. Kaine ex-Finnigan Technical Specialist ****************************************************************************** From: "Karl J. Treier of UniTech" Date: 15 Jan 1997 14:58:05 GMT Subject: Teknivent Where are you ? Organization: UniTech I was trying to reach the Teknivent website and it appears to have gone. Has the company ? -- UniTech 680 Northland Boulevard Suite 9 Cincinnati OH 45240, USA Phone: 1-800-989-0144 / (513) 825-0144 Fax: (513) 825-0163 E-Mail: info@uni-tech.com Web site: http://www.uni-tech.com ****************************************************************************** From: Christopher Kaine Date: Wed, 15 Jan 1997 19:40:33 -0800 Subject: Re: Thank-you Finngan Customers Organization: OneNet Communications HUB News Server Christopher Kaine wrote: } } Finngan Customers: } } It has been my pleasure to assist and train some of you during my 8 year } tenure with Finnigan. It is Finnigan management's plan that my and other } educated/trained and experienced mass-spectroscopist Technical } Specialist's services are no longer required for the customers or Service } Engineers. I will though, continue to avail myself to this newsgroup to } offer what ever expertise I may. } } Again, I thank-you and wish you all the best in the coming years. } } Cordially; } } Christopher J. Kaine } ex-Finnigan Technical Specialist Sorry, my typing leaves something to be desired. Should be Finnigan ****************************************************************************** From: kd4wyn@emi.net Date: 16 Jan 1997 14:17:26 GMT Subject: FT-IR & Epoxy - HELP Organization: The EmiNet Domain (407)731-0222 Does FT-IR comparasion really give an accurate "fingerprint" of a material such as a two component epoxy such that the FT-IR results could be used as a stand alone quality verification of the material? Example: We identify a specific batch of coating that has the desired properties we want a manufacturer to reproduce from batch to batch. We have the initial batch "fingerprinted" by FT-IR and use that spectra as the "master fingerprint" to which all subsequent batches are examined by FT-IR and compared to the master. If the spectra is "reasonably identical", does that alone assure us that the coating is really the same as the master batch? Are there other lab tests that would give us a higher degree of confidence? Thank you very much! Michael Crapps Corporate Quality Assurance Manager S.G. Pinney & Associates, Inc. Port St. Lucie, FL ****************************************************************************** From: John Callahan Date: Thu, 16 Jan 1997 11:17:48 -0500 Subject: Re: Teknivent Where are you ? Organization: Code 6113/Naval Research Laboratory Karl J. Treier of UniTech wrote: } } I was trying to reach the Teknivent website and it appears to have gone. } Has the company ? } Teknivent has been acquired by ProLab resources. They can be reached at: ProLab Resources, Inc. prolab@ix.netcom.com http://www.prolab.com (608) 278-0002 (608) 278-0072 FAX -- John H. Callahan Code 6113/Analytical Chemistry Section Naval Research Laboratory Washington, D.C. 20375 202-767-0719 FAX 202-404-8119 John.Callahan@nrl.navy.mil http://chem1.nrl.navy.mil/analytical ****************************************************************************** From: Chad Nelson Date: Thu, 16 Jan 1997 16:56:15 -0500 Subject: Re: FT-IR & Epoxy - HELP Organization: Advanced Fuel Research, Inc. kd4wyn@emi.net wrote: } } Does FT-IR comparasion really give an accurate "fingerprint" of a material } such as a two component epoxy such that the FT-IR results could be used as } a stand alone quality verification of the material? } } Example: } We identify a specific batch of coating that has the desired properties we } want a manufacturer to reproduce from batch to batch. We have the initial } batch "fingerprinted" by FT-IR and use that spectra as the "master } fingerprint" to which all subsequent batches are examined by FT-IR and } compared to the master. If the spectra is "reasonably identical", does } that alone assure us that the coating is really the same as the master } batch? Are there other lab tests that would give us a higher degree of } confidence? } } Thank you very much! } } Michael Crapps } Corporate Quality Assurance Manager } S.G. Pinney & Associates, Inc. } Port St. Lucie, FL FT-IR can give you very reproducible information regarding the chemistry of the batch (ie relative concentration of molcular species or covalent bonds/functional groups) but will not tell you how well the coating is bonded or what the structural properties of the material are. -- Sincerely, Chad Nelson Advanced Fuel Research, Inc. 87 Church Street East Hartford, CT 06108-3742 "The views expressed above are those of the author and do not necessarily reflect the views of Advanced Fuel Research, Inc. or any affiliates." ****************************************************************************** From: Paul Slater Date: Fri, 17 Jan 1997 12:27:28 0000 Subject: Electrospray research in Australia Organization: University of Wales Swansea Dear sir/madam, My name is Paul Slater, and I am currently completing my Ph.D. in novel applications of electrospray mass spectrometry. My main area of interest is in the use of organic adducts to improve the quality of salt contaminated spectra and to quantify the number of polar groups, (for example, sulfonates), that are present. I have achieved this goal with a wide range of complex polysulfonated compounds, mainly azo dyes. In October my partner and I will be moving to Australia for approximately one year. During this period I hope to find work or research in my field. I would be grateful if you could therefore advise me on some contacts whom I can approach and arrange a possible visit. Thank you in advance for your time and help. With very best wishes, Paul Slater EPSRC National Mass Spectrometry Service Centre, Department of Chemistry, University of Wales Swansea, Singleton Park, Swansea SA2 8PP. U.K. ****************************************************************************** From: J K Brandau Date: Fri, 17 Jan 97 09:19:27 PST Subject: Losing GC column pressure Organization: * Our Varian 3400CX gas chromatograph coupled to out Saturn 3 GC/MS has a 1077 Split/Splitless injector. After a BTEX run on the Tekmar 2016 autosampler, the backpressure gauge on the GC went to zero! We notice when tripping the GC into run mode, the pressure returns. The pressure is lost again when GC returns to ready mode. We are having a difficult time identifying the problem. Has anyone had a similar problem? Thanks, Ken Brandau ------------------------------------- Name: J K Brandau E-mail: J K Brandau Date: 1/17/97 Time: 9:19:27 AM This message was sent by Chameleon ------------------------------------- ****************************************************************************** From: Eric Nordstrom Date: Sat, 18 Jan 1997 00:28:00 -0800 Subject: Nondestructive Oriented Website Organization: Micron Internet Services To whom it may concern, My name is Eric Nordstrom. I am a Nondestructive Inspection Specialist within the U.S. Air Force. This note is designed to notify individuals within the Nondestructive Community that I have a homepage posted designed to allow easy access to hard-to-find information, other web sites, newsgroups, and employment all Nondestructive Oriented. Please pay a visit to "http://netnow.micron.net/~dozod/". Send me comments and suggestions via: dozod@micron.net. Thank you, E.G.Nordstrom ****************************************************************************** From: "Rick Flurer" Date: 19 Jan 1997 04:02:01 GMT Subject: Re: rat poison Organization: home Brodifacoum can be detected quite nicely by positive ion APCI using an acetonitrile/water gradient. Use a C18 column and add 0.1 % formic acid to moble phase to suppress ionization of the acidic enol group. Tandem MS on MH+ ion is quite useful for i.d. proof. regards, rick flurer phil harris wrote in article <59e6q3$c34@acmey.gatech.edu>... } Im trying to set up an LC-MS method for the analysis of a rat poison } (brodifacoum) but have found that in ESI the chromatogram is inverted ie } the analyte apparently suppresses the background ion current. The } effect is the same in positive and negative mode, and I havent tried } APCI yet to see if the effect is the same. Is there a way around this } to get my chromatograms the right way up?! } } } } } ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 19 Jan 1997 18:32:49 GMT Subject: Re: 3-Hydroxy-palmiticacid Organization: AOL http://www.aol.com In the process of investigating the fatty acid synthesis pathway of bacteria I was forced to synthesize 3-Hydroxy-Decynoyl NAC(ie you might not be able to buy it) I am pretty sure that the enzyme pathways are stereospecific for one isomer over another. The Cycle of FA synthesis produces the 3-0H configuration . This is stereospecific in E.Coli (and most others I'd imagine)Because the subsequent step in which the 3-OH form is utilized has a stereospecific requirement. I Quote; Beta-Ketoacyl-ACP reductase The reaction product of acetoacetyl-ACP has the D(-) configuration. Beta-Hydroxyacyl-ACP dehydrasae The enzyme (beta-hydroxyacyl-ACP dehydrase) catalyses the dehydration of D(-)-beta-hydroxyacyl-ACP thioesters to form trans-2-enoyl-ACP thioesters... Like the beta-ketoacyl-ACP synthetase, the dehydrase has an absolute specificity for thioesters of ACP; it is inactive with thioesters of CoA or pantetheine. The reaction is stereospecific; the D(-) isomer of beta-hydroxybutryl-ACP was dehydrated whereas the L(+) isomer was not. pg 123 Quotes from Biochemistry of Lipids Ed. TW Goodwin, F.R.S. Vol. 4 of the MTP International Review of Science Series 1974 ISBN 0-8391-1043-X Butterworth and Co. Pub. You might also check out Morris Kates' "techniques of lipidology" Elsevier (2nd ed 1986) This guy is the GOD of lipids in my opinion. he says on pg 34 "The b-hydroxy saturated acids form a series of even-carbon acids...3-Hydroxy acids are fairly widespread in yeasts and bacteria, usually occuring as D(-) isomers in ester bound or glycosidic forms in extracellular lipids. For example, 3-D-OH-C10,-C12,-C14 acids are constituents of lippopolysaccharides in cell walls of Gram-Negative Bacteria (Kaneshiro & Marr 1963 Biochim.Biophys.Acta, 70,271); 3-D-hydroxy-C10 acid occurs in the rhamno-lipid of Pseudomonas aeruginosa (Jarvis and Johnson 1949, JACS, 71,4124)...(another ref...Tulloch and Spencer1964, Can.J.Chem.42,830)..The D-3-OH acids are also intermediates in the beta-oxidation pathway of fatty acids." Many people (eg. Julie Leary et al) have been using the addition of Metals to differentiate various types of isomers using mass spec. If you have some standards in D and L forms and you have a mass spec you could develop a method. Otherwise maybe one of the old papers have methods (bucket loads of sample ?) Source of the material combined with literature info may tell you indirectly the D/L of what you have. John Cronan is another awesome lipid guy (U Ill ?) good luck Matt Sweeney good luck ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 19 Jan 1997 18:42:18 GMT Subject: Re: 204 contaminant Organization: AOL http://www.aol.com You might try putting it on a column and seeing what the chromatographic profile of the contaminant versus the peptide is. It'd give you some clues. It'd also get the peptide away form the garbage. (a guess >>> Mannitol/Inositol +Na = 203.2 ?) ****************************************************************************** From: adamf@vtaix.cc.vt.edu (Adam Finkelstein) Date: 20 Jan 1997 17:02:37 GMT Subject: FID with Shimadzu GC14A --Sensitivity problems Organization: Virginia Tech Hello. I'm using a Shimadzu GC 14A with FID and an RTX 5 capillary column. I have optimized all my parameters and have checked with Shimadzu and they concur. They sent me very arcane instructions on cleaning the detector. I am planning to replace the nozzle, but would like to avoid cleaning the detector if possible: it seems very detailed and laborious. Has anyone cleaned an FID detector like this? If so would you mail or post about your experience? thanks! Adam -- _________________ Adam Finkelstein adamf@vtaix.cc.vt.edu http://sunsite.unc.edu/bees/adamf/home.html ****************************************************************************** From: Corinne Bure Date: Mon, 20 Jan 1997 20:48:46 +0100 Subject: Patchouly's component Organization: Grolier Interactive Europe I m an student in Spectrochemistry and my subject's trainee is the study of an Essential Oil of Patchouly by Mass Spectrometry coupled with gas chromatography. So, I search for mass spectra of the Patchouly oil like ... - Patchoulenone M=218 C15 H22 O - Isopatchoulenone M=218 C15 H22 O - Norpatchoulenol M=206 C14 H2 O - Pogostol M=222 C15 H26 O - 1 alpha - 5 alpha Epoxy - alpha Guaiene M=220 C15 H24 O - 1 beta - 5 beta Epoxy - alpha Guaiene M=220 C15 H24 O - 1,10 Epoxy alpha Bulnesene M=220 C15 H24 O - alpha Patchoulone M=220 C15 H24 O - beta Patchoulone M=220 C15 H24 O - 5 oxo Patchoulol M=236 C15 H24 O2 - alpha Guaienone M=218 C15 H22 O - alpha Cedrenal M=218 C15 H22 O - beta Patchoulenone M=218 C15 H22 O - gamma Selinene ? - Pogostone M=224 C12 H16 O4 - alpha Elemene M=204 C15 H24 - Beta Bulnesene M=204 C15 H24 Thank you for your help. ****************************************************************************** From: SCROOD Date: Mon, 20 Jan 1997 14:19:31 +0000 Subject: amine analysis Organization: Animal Sciences, Univ. of Illinois has anyone had any luck with amine analysis using mass spec? any advice would be appreciated. pete ****************************************************************************** From: gbird@superlink.net Date: Tue, 21 Jan 1997 00:55:22 GMT Subject: Re: amine analysis Organization: SuperNet Inc. (908) 828-8988 On 20 Jan 1997 15:29:27 -0500, SCROOD wrote: }has anyone had any luck with amine analysis }using mass spec? } }any advice would be appreciated. } } }pete Sure I have Pete, what kind of amines are you looking at? } ****************************************************************************** From: SCROOD Date: Tue, 21 Jan 1997 08:59:11 +0000 Subject: Re: amine analysis Organization: Animal Sciences, Univ. of Illinois gbird@superlink.net wrote: } } On 20 Jan 1997 15:29:27 -0500, SCROOD } wrote: } } }has anyone had any luck with amine analysis } }using mass spec? } } } }any advice would be appreciated. } } } } } }pete } } Sure I have Pete, what kind of amines are you looking at? } } } i don't know i need a little advice on sample prep. (pH...etc.) and the proper column and column temp. does this help? pete ****************************************************************************** From: Ian Michael Date: Tue, 21 Jan 1997 15:22:33 +0000 Subject: European Mass Spectrometry Issue 2/6 Organization: IM Publications Distribution: world The complete abstracts of issue 2/6 of European Mass Spectrometry are now available on our web site: http://www.impub.co.uk These abstracts and those of all previously published issues are searchable. If anyone cannot access the WWW and would like a text version e-mailed to them, please send a message to me. -- Ian Michael IM Publications Publishers of: 6 Charlton Mill European Mass Spectrometry Charlton Spectroscopy Europe Chichester Journal of Near Infrared Spectroscopy West Sussex PO18 0HY NIR news United Kingdom Tel: +44-1243-811334 Fax: +44-1243-811711 E-mail: ian.michael@impub.demon.co.uk Web site: http://www.impub.co.uk ****************************************************************************** From: dmichaud@phy.ulaval.ca Date: 21 Jan 1997 18:43:29 GMT Subject: tmpump grease Organization: Universite Laval Distribution: world Do there exist alternate suppliers of ball bearing grease for Alcatel turbomolecular pumps? We are finding out how costy the syringe refills are... Thank you, Daniel Michaud Departement de physique Universite Laval ****************************************************************************** From: "Joe Bumgarner, Jr." Date: Tue, 21 Jan 1997 13:52:28 -0500 Subject: Re: Teknivent Where are you ? Organization: SciQuest Karl J. Treier of UniTech wrote: } } I was trying to reach the Teknivent website and it appears to have gone. } Has the company ? } } -- } UniTech } 680 Northland Boulevard } Suite 9 } Cincinnati } OH 45240, USA } } Phone: 1-800-989-0144 / (513) 825-0144 } Fax: (513) 825-0163 } E-Mail: info@uni-tech.com } Web site: http://www.uni-tech.com I found this company information, TEKNIVENT CORP., MARYLAND HEIGHTS, MO at SciQuest (http://sciquest.com). You can send them a email directly from the web site. Good Luck, ____________________________________________________________________ Joe Bumgarner, Jr. SciQuest - "Internet Solutions for Science" jbumgarner@sciquest.com "http://sciquest.com" Site Director Phone: 919-732-5813 FAX: 919-782-3123 ____________________________________________________________________ ****************************************************************************** From: Chris Elhardt Date: 21 Jan 1997 22:48:33 GMT Subject: Re: Losing GC column pressure Organization: Texas Department of Health You should have a septum purge restriction in the system; sounds like it could be either missing (if you have the fixed restrictor) or wide open (if you have the valved restrictor) a) fixed restriction: Look for a nickel line from the injector block terminating in a brass union. The union may be painted either green or blue depending on the restriction. Make sure the restriction is present and tightened. b) variable restriction: Look for a vernier to the right of the injector (there may be a pair of them if you have both split/splitless and a SPI injector installed. ) Measure the flow at the valve; should be about 1 ml/min/pound of head pressure; adjust accordingly. c) check your gc program in the analysis-page/gc edit. select the set parameter menu item and make sure that you've got your relays the way you want them. sounds like you may be switching to split during desorb and splitless during the run. Also check that the quarter-inch graphite seal around the injector liner is undamaged. It's only good for about 4-5 liners and then should be replaced. chris J K Brandau wrote: }Our Varian 3400CX gas chromatograph coupled to out Saturn 3 GC/MS has a }1077 Split/Splitless injector. } }After a BTEX run on the Tekmar 2016 autosampler, the backpressure gauge on }the GC went to zero! } }We notice when tripping the GC into run mode, the pressure returns. The }pressure is lost again when GC returns to ready mode. } }We are having a difficult time identifying the problem. Has anyone had a }similar problem? } }Thanks, } }Ken Brandau }------------------------------------- }Name: J K Brandau }E-mail: J K Brandau }Date: 1/17/97 }Time: 9:19:27 AM } }This message was sent by Chameleon }------------------------------------- } } } ****************************************************************************** From: charland Date: Wed, 22 Jan 1997 22:12:06 GMT Subject: compound type distribution from HP GC/MS chemstation software library search listing Organization: Natural Resources Canada (NRCan) The library search routine from the HP chemstation software driving an HP GC-MSD system produces an output listing with hits for each chromatogram peak using the mass spectrum collected at that retention time. This provides useful data but does not allow you to have an overall compound type distribution or I should say a way of classifying the best hits according to their chemical type, i.e., ketones, aromatics, carboxylic acids, paraffin, etc. Anybody heard of some software that would use such output listing to classify compounds into organic types and provide an overall type distribution? This could be useful if you are to look at and assess various types of oils like petroleum crudes, derived oil fractions or pyrolysis oils. I Hope this will attract some comments and feedback. Thank you ****************************************************************************** From: plaut@sc2a.unige.ch (Olivier PLAUT) Date: Thu, 23 Jan 1997 10:18:42 +0200 Subject: Re: compound type distribution from HP GC/MS chemstation software Organization: University of Geneva, Switzerland In article <5c5pc5$fjk@acmex.gatech.edu>, charland writes: } The library search routine from the HP chemstation software } driving an HP GC-MSD system produces an output listing } with hits for each chromatogram peak using the mass spectrum } collected at that retention time. This provides useful data } but does not allow you to have an overall compound type distribution } or I should say a way of classifying the best hits according to their } chemical type, i.e., ketones, aromatics, carboxylic acids, } paraffin, etc. Anybody heard of some software that would use } such output listing to classify compounds into organic types } and provide an overall type distribution? This could be useful } if you are to look at and assess various types of oils like } petroleum crudes, derived oil fractions or pyrolysis oils. } } I Hope this will attract some comments and feedback. } Thank you } In the libraries, you have a "Misc information" field. If you have your own library, you can put in the chemical type and then modify the PBM macro to get selected type answers only. Of course, if you want to modify a very large existing library, it is a hard work. Olivier -- ------------------------------------------------------------------- Olivier Plaut | Institute of Forensic Medicine | Phone: +41 (22) 702.56.09 Toxicology Unit | Fax: +41 (22) 789.24.17 University of Geneva | Av. de Champel 9 | E-mail: plaut@sc2a.unige.ch CH-1211 Geneve 4 | Switzerland | ------------------------------------------------------------------- ****************************************************************************** From: "Lewis K. Pannell" Date: Thu, 23 Jan 1997 12:21:57 EST Subject: Post Doctoral Appointment Available in NIDDK, NIH Organization: * The Structural Mass Spectrometry group at NIDDK, NIH, has an opening available for a post doctoral candidate. Work will largely concentrate on mass spectrometry as applied to biopolymers but the laboratory also does a large amount of structural identification on smaller compounds with potent specific biological activity. Please refer to http://sx102a.niddk.nih.gov/lewisjob.html for more information. -- Dr. LEWIS K. PANNELL Bldg 8, Rm B2A23, LBC, NIDDK National Institutes of Health Bethesda, MD 20892-0805, USA. Ph (301)402-2196 FAX (301)402-1967 Email: L_Pannell@nih.gov ****************************************************************************** From: phil harris Date: 24 Jan 1997 05:02:49 GMT Subject: rat poison again Organization: Biomedical Mass Spec Unit, UNSW, Sydney In a reply to my previous query, someone responded with reference to/experience of APCI in the study of brodifacoum, but I couldn't contact them to find out more. I'd be grateful if he could contact me and/or let me know if the work has been published. Thanks in advance. ****************************************************************************** From: "Lee, Ji Hyun" Date: Fri, 24 Jan 1997 14:08:39 +0900 Subject: MALDI-TOF is suitable to identify whether a protein is a dimer or a trimer? Organization: College of Pharmacy, Seoul National University MALDI-TOF analysis is suitable to identifying whether a protein is a homodimer or a homotrimer? I have a small DNA-binding protein(6,100 dalton) quite well purified. Through size exclusion HPLC gel chromatography, I found its effective molecular weight of the protein in the absence of its binding DNA sequence is about 19,000 dalton, close to homotrimer. But I am not sure that it is a trimer based on only the result becuase I didn't ever hear about homotrimer DNA binding protein; so I need some additional experiments confirming this fact. Thank you for your time and expertise, Lee, Ji Hyun ****************************************************************************** From: heino.prinz@mpi-dortmund.mpg.de (Heino Prinz) Date: Fri, 24 Jan 1997 14:47:35 GMT Subject: Re: MALDI-TOF is suitable to identify whether a protein is a dimer or a trimer? Organization: MPI-Dortmund On 24 Jan 1997 08:13:51 -0500, "Lee, Ji Hyun" wrote: }MALDI-TOF analysis is suitable to identifying whether a protein is a }homodimer or a homotrimer? } }I have a small DNA-binding protein(6,100 dalton) quite well purified. }Through size exclusion HPLC gel chromatography, I found its effective }molecular weight of the protein in the absence of its binding DNA }sequence is about 19,000 dalton, close to homotrimer. }But I am not sure that it is a trimer based on only the result becuase I }didn't ever hear about homotrimer DNA binding protein; so I need some }additional experiments confirming this fact. } You probably should run the protein on an electrospray mass spectrometer. regards, Heino Prinz Tel.:(+49)-231-1206-332 Fax:(+49)-231-1206-389 ****************************************************************************** From: "Alex" Date: 24 Jan 1997 19:53:25 GMT Subject: Filament power supply floating at 12kV needed Organization: Swiss Federal Institute of Technology (ETHZ) Hello, Anyone ever had a problem of finding a filament power supply for the electron gun which would be capable of floating at +/-12kV and providing up to 5Amps regulated current at 0-5V? Any companies who manufacture that? Please reply to alik@erdw.ethz.ch. Alexander Likholyot -- My real email was altered to prevent junk mail. ****************************************************************************** From: sismspec@aol.com (Sismspec) Date: 26 Jan 1997 22:02:13 GMT Subject: Position Available - Scientific Product Manager Organization: AOL http://www.aol.com Position available at Scientific Instrument Services in ringoes, NJ. Scientific Product Manager to provide technical support for our MS, GC, LC, Vacuum fittings and Thermal Desorption product lines. This includes phone support with customers, attending trade shows, operating our mass spectrometers and accessory equipment to solve customer problems or provide customers with applications, and aiding in the development of new products. This person will be responsible to answer customer technical questions relating to the products we sell, repair or manufacture. This person will also help customers with the maintenance of their mass spectrometers, gas chromatographs, liquid chromatographs and other scientific instruments utilizing our parts and services and provide customer technical support. The position is at our facilities in Ringoes, NJ with minimal travel, restricted mainly to several trade shows. The second function of this position is to work as part of the SIS new product development team. This involves the development of new GC/LC/MS accessories, supplies, techniques and services which will be marketed to the scientific market. We are presently beginning the development of new LC/MS equipment and therefore a good LC background will be necessary for this position. In addition this person will develop ideas for new products and services we can market from other vendors and suppliers as well as from our own manufacturing facilities. The will evaluate new products from our distributors and incorporate them into our product line and solicit new products and dealerships. For more information, contact John Manura at (908)788-5550 or via E-Mail at sis@sisweb.com or visit our web site at http://www.sisweb.com/index/referenc/sisemplo.htm for more information. John J. Manura Scientific Instrument Services Supplies and Services for Mass Spectrometers, Gas Chromatographs and related Scientific Instruments SIS Home Page: http://www.sisweb.com E-Mail: sismspec@aol.com Phone: (908) 788-5550 FAX: (908) 806-6631 ****************************************************************************** From: Clair Bragg Date: Sun, 26 Jan 1997 18:16:13 -0800 Subject: HP 5970 Organization: GenTech Scientific, Inc. Dear HP mass spec user, We currently have a 5970 with Pascal Chem Station. We would like to sell or trade it. If you still have a need please contact GenTech Scientific at 716-631-1022. Sincerely, Clair Bragg ****************************************************************************** From: Tim Pope Date: 27 Jan 97 01:49:14 GMT Subject: hydrogen fluoride detection Organization: TotalNet Inc. I'm trying to determine [HF] gas concentration between .5ppm and 120ppm--can this be done quantitatively with a quadrapole mass spec? I'd appreciate any comments, hints or ideas! Thanks, Marci Vernon ****************************************************************************** From: Don Magin Date: Mon, 27 Jan 1997 15:01:05 -0800 Subject: Hickory flavor Organization: Whitehall-Robins Analytical Research Does anyone know of a compilation of compounds (and their mass spectra) which are typically found in hickory flavor? In a more general sense, can anyone point me toward some reference books or articles which list the compounds which might be expected to be found in flavors, oils, and perhaps other such common products such as cleaning fluids, fuels, etc. We sometimes have the occasion to try to identify such materials in adulterated or mis-handled products. ****************************************************************************** From: pggreen@cco.caltech.edu (Peter G. Green) Date: Mon, 27 Jan 97 20:42:12 GMT Subject: Job Opening Organization: Env. Eng. Sci., Caltech This Thursday, January 30th, 1997, the following announcement of a job opening will appear at Caltech's Human Resources list of employment opportunities http://www.cco.caltech.edu/~employ/posting.html at which time one may telephone them for official application information. ASSISTANT SCIENTIST Env. Eng. Science Will perform assistance in all operations of the Environmental Analysis Center (elemental and organic mass spectrometries and chromatographies). Responsibilities will include: routine maintenace; problem solving and troubleshooting; assuming full responsibility during Scientist's absences; applications of standard techniques; and instructing users and assisting researchers. Reqs. BS degree in Earth or Environmental Science or Chemistry and min. 6 mos. related exp. or equivalent. Must possess exp. with PC's (hardware, software, networking accessories, including multimedia), and general electrical and mechanical skills. Must be able to prioritize, work independently, and possess excellent interpersonal and communication skills. Candidates must submit a cover letter, resume and names of 3 references with phone numbers and/or e-mail address. ****************************************************************************** From: yamx0001@gold.tc.umn.edu Date: Mon, 27 Jan 1997 14:46:29 -0500 Subject: LDV - schematics ?? Organization: University of Minnesota I am a physics student at the U of MN. I'm developing an experiment and need to implement a Laser-Doppler Velocitometer (LDV), in order to measure the velocity of Couette-Taylor fluid flow. I only need to measure 1 dimension, and don't need anything fancy of sophisticated. Can anyone help me out with schematics for transmitting and receiving circuits, and interfacing with a PC? I have lasers. I just don't know how to design the circuits. Please post or email me would be better. Thanks. Patrick yamx0001@gold.tc.umn.edu ****************************************************************************** From: winnik@charlotte.med.nyu.edu (Witold Winnik, Ph.D.) Date: Tue, 28 Jan 1997 09:16:49 -0500 Subject: LC/GC MS/MS mass spectrometers Organization: * Hello, I am trying to find a mass spectrometer which can perform the following tasks: 1. reasonably easily switches between GC/MS and LC-electrospray jobs (one week it would perform a GC/MS analysis and following weeks LC/MS, for example) 2. capable of EI / CI and positive / negative ionization 3. performs MS and MS/MS analysis; mass range up to 3000-4000 m/z And costs below $400,000. I know that Finnigan GCQ and LCQ ion traps would qualify jointly but the problem is I can request only one instrument in my proposal, even though it would be probably less expensive to aquire both GCQ and LCQ instead of one instrument that can switch between GC and LC jobs. If anyone knows about such instrument, or has been using it please contact me at: winnik@charlotte.med.nyu.edu Witold Winnik NYU Medical Center ****************************************************************************** From: Bill Cotham Date: Tue, 28 Jan 1997 09:36:01 -0500 Subject: Re: LC/GC MS/MS mass spectrometers Organization: USC Mass Spectrometry Center Witold Winnik, Ph.D. wrote: } } Hello, } } I am trying to find a mass spectrometer which can perform the following tasks: } 1. reasonably easily switches between GC/MS and LC-electrospray jobs (one } week it would perform a GC/MS analysis and following weeks LC/MS, for } example) } 2. capable of EI / CI and positive / negative ionization } 3. performs MS and MS/MS analysis; mass range up to 3000-4000 m/z } And costs below $400,000. } } I know that Finnigan GCQ and LCQ ion traps would qualify jointly but the } problem is I can request only one instrument in my proposal, even though it } would be probably less expensive to aquire both GCQ and LCQ instead of one } instrument that can switch between GC and LC jobs. } If anyone knows about such instrument, or has been using it please contact } me at: winnik@charlotte.med.nyu.edu } } Witold Winnik } NYU Medical Center I think a Finnigan TSQ or a Micromass Quatro would meet all of your specs. -- Bill Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry and Biochemistry University of South Carolina Columbia, SC 29208 803-777-2039 (voice) 803-777-9521 (fax) ****************************************************************************** From: JGRAFF@PILLSBURY.COM Date: Tue, 28 Jan 1997 13:56:09 -0600 Subject: 5890/5970 GC/Mass Spec Organization: * I have a 5890/5970 with 2 pacal workstations for sale. If you are interested please contact me. I'm looking for approx $25000 but am willing to talk. You can E-mail me at -- jgraff@pillsbury.com. ****************************************************************************** From: ajohnson@chemistry.adelaide.edu.au (Andrew Johnson) Date: Wed, 29 Jan 1997 20:40:45 +1030 Subject: ESQUIRE User Interface Organization: The University of Adelaide Has anyone out there use the new user interface for the Bruker ESQUIRE , which is supposed to be based on HP's ChemStation HPLC software ( and looked like a VB interface to me from pix ) ? Or perhaps even compared it to a Finnigan LCQ ? ( I'm aware of the technical specifications of the respective machines, but I was curious as to the impressions from the user interfaces , and the reliability and stability of the PC based software ) -- Andrew Johnson Email: ajohnson@chemistry.adelaide.edu.au _-_|\ Dept of Chemistry Phone: +61 8 3033241 / \ The Uni of Adelaide Fax: +61 8 3034381 \_.-*_/ AUSTRALIA v ****************************************************************************** From: cgek@cc.newcastle.edu.au (E M Kennedy) Date: Thu, 30 Jan 1997 09:12:33 +1100 Subject: Kratos spares Organization: Chemical Engineering, Newcastle univ (Australia) Dear group, Does anyone have spares for Kratos MS25/80 which they are intersted in selling? Im mainly interested in spares for the source and data system. Please contact me for details Eric Kennedy cgek@cc.newcastle.edu.au Ph +61 - 49 - 21- 6177 Fax +61 - 49 - 21 -6920 Thanks ****************************************************************************** From: tswenson@ral.mindspring.com (SpectraSource) Date: Wed, 29 Jan 1997 19:59:24 -0200 Subject: WANTED: 5989A MS Engine Organization: SpectraSource, Inc. We are looking for a used 5989A MS Engine, and will pay a good price, especially if the instrument has many of the options we seek. Please respond by e-mail or call (919) 571-0940 ****************************************************************************** From: David Sparkman <75530.3472@compuserve.com> Date: Wed, 29 Jan 1997 21:43:00 -0500 Subject: Library Search Organization: * On 22 Jan, charland of Natural Resources Canada (NRCan) wrote: {The library search routine from the HP chemstation software {driving an HP GC-MSD system produces an output listing {with hits for each chromatogram peak using the mass spectrum {collected at that retention time. This provides useful data {but does not allow you to have an overall compound type distribution {or I should say a way of classifying the best hits according to their {chemical type, i.e., ketones, aromatics, carboxylic acids, {paraffin, etc. Anybody heard of some software that would use {Such output listing to classify compounds into organic types {And provide an overall type distribution? This could be useful {If you are to look at and assess various types of oils like {Petroleum crudes, derived oil fractions or pyrolysis oils. The NIST Mass Spectral Search Program for Windows offers substructure identification; which is what I think you want. The program will examine the best 100 spectra from the libray search of an unknown and determine the probablity of the presence and absences of various substructure features of the spectrum that was searched. This includes things such as aldehydes, ketones, acids, ring-plus-double-bonds, hetroatoms, aromatic nature, etc. This is what you are looking for. This program will work interactively with the HP software. You can buy it from WindChem Software in Fairfield, CA (1-800-536-0404) for $1,495 with the NIST/EPA/NIH database of about 75,000 spectra. If you already have the HP NBS75K.L database, the price is $750. Regards; O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: Federico Pea Date: Thu, 30 Jan 1997 16:00:49 +0100 Subject: Tamoxifen and its metabolites Organization: Institute of Clinical Pharmacology and Toxicology - University of Udine - Italy Hi, we are looking for an HPLC-LC/MASS method to separate tamoxifen and its major metabolites. Thanks in advance -- Federico Pea, MD Institute of Clinical Pharmacology and Toxicology University of Udine P.le S.M. Misericordia, 3 33100 Udine Italy e-mail : furlanut@hydrus.cc.uniud.it web: http://www.uniud.it/ifct/welcome.html ****************************************************************************** From: "Joe Bumgarner, Jr." Date: Thu, 30 Jan 1997 16:10:07 -0500 Subject: Vendor Search/Communication Tool Organization: SciQuest To locate and communicate with vendors of virtually any scientific product or service, go to http://sciquest.com. Conduct a keyword search of our database of over 5000 vendors, then broadcast your request to all the vendor(s) that sell that product. It's completely FREE and will save you time and expand your purchasing options. Best Regards, ____________________________________________________________________ Joe Bumgarner, Jr. SciQuest - "Internet Solutions for Science" jbumgarner@sciquest.com "http://sciquest.com" Site Director Phone: 919-732-5813 FAX: 919-782-3123 ____________________________________________________________________ ****************************************************************************** From: Massimo Baraldo Date: Fri, 31 Jan 1997 12:07:55 +0100 Subject: tamoxifen metabolites (II) Organization: Cineca HELP For analytical purpose I am looking for few miligrams of tamoxifen metabolites (N-desmethyl-tamoxifen ; N,N-didesmethyl-tamoxifen; 4-OH-tamoxifen; isomers of 4-OH-tamoxifen; tamoxifen-N-oxide). Where can I found those samples or does anybody know who can synthesize them? It seems that they are not commercialy available. -- Massimo Baraldo, MD Institute of Clinical Pharmacology and Toxicology University of Udine P.le S.M. Misericordia, 3 33100 UDINE Italy e-mail: furlanut@hydrus.cc.uniud.it web: http://www.uniud.it/ifct/welcome.html ****************************************************************************** From: David Sparkman <75530.3472@compuserve.com> Date: Fri, 31 Jan 1997 09:42:39 -0500 Subject: LC-GC/MS Instrument Organization: * On Tuesday January 28 Witold Winnik, Ph.D. wrote looking for an instrument that will do EI/CI GC/MS and can be switched to ESI LC/MS. One of the best instruments for this is the Finnigan/ATI Automass built in Paris. This instrument was desiged to work along these lines. It is currently being sold by Finnigan out of Austin, Texas. O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: cip1591@iperbole.bologna.it (Ermanno Errani) Subject: GC-MS of pesticides: help on general rules Date: Thu, 30 Jan 1997 21:28:27 GMT Organization: NETTuno I am interested in pesticides residues analysis in foods: every time I find something by GC-ECD or NPD, I try to confirm it by GC-MS with quadrupole (SIM), in the case I already know the mass spectrum of the hypotised compound. Otherwise, I must try to get a complete scan (total ion) for a part of the chromatogram, and watch the scan of each peak detected (finding Hydrocarbons, waxes, terpenes, acids, lots of naturally occurring compounds). The library search for matching probability often gives me very strange kind of molecules with little meaning, and the total ion scan lack the sensitivity needed for the detection of p.p.m. amounts of contaminants. I could concentrate 100- to 1000-fold the extract, with the sure result of great conyamination of liner, column, and even ion source of quadrupole. Is there a rule for monitoring groups of compounds (e.g. fungicides, phosphoric esters, halogenated compounds) by SIM of few peaks (up to 20) and with a minimum number of injections ? Thanks for every kind of suggestions ! Ermanno Errani ****************************************************************************** From: david.bostwick@chemistry.gatech.edu (David Bostwick) Date: 31 Jan 1997 16:17:02 GMT Subject: ADMIN: Computer down time Organization: Georgia Tech The computer that handles the newsgroup will be down Monday, February 3, beginning at 0400 EST, for the installation of a UPS. It should be back up by late Monday afternoon. ****************************************************************************** From: lfu@tamu.edu (Liqun Fu) Date: Fri, 31 Jan 1997 20:23:48 GMT Subject: Mass calibration and SIS Organization: Texas A&M Univ. Hi, All, Is anyone out there familiar with Mass calibration and SIS for a GC/MS system? The mass calibration is based on FC-43. The peaks at masses 69, 131, 264, 414, 464, 502 were found, but mass at 614 was not found. The slope of the calibration was 6.300 with a standard deviation of less than 0.150. With such conditions, will the SIS function properly? If not, how to correct it? The molecular weights of all the compounds which need to be analyzed are below 400 and the system works fine with EI and no SIS. Thanks in advance, Liqun Fu, Graduate assistant Soil and Crop Sciences lfu@tamu.edu Texas A&M Univ. (409) 845-7654 ****************************************************************************** From: U61985@UICVM.UIC.EDU Date: Fri, 31 Jan 1997 14:45:35 CST Subject: Matrix solutions for MALDI Organization: University of Illinois at Chicago, ADN Computer Center Can anyone tell me who sell matrix solutions for maldi? I know HP makes those solutions but I don't have their catolog. Any informations are welcome. ****************************************************************************** From: Jeffrey Craig Thomas Date: Fri, 31 Jan 1997 21:24:56 -0500 Subject: Re: Matrix solutions for MALDI Organization: iWAYnet Communications U61985@UICVM.UIC.EDU wrote: } } Can anyone tell me who sell matrix solutions for maldi? I know HP makes } those solutions but I don't have their catolog. Any informations are } welcome. HP's analytical tech support number is 1-800-424-9759 -- Jeffrey C Thomas Columbus, Ohio. USA http://www.iwaynet.net/~jcraig email: jcraig@iwaynet.net ****************************************************************************** From: "Kiley R. Prilliman" Date: Sun, 02 Feb 1997 19:28:05 -0600 Subject: HPLC-RP column selection--question! Organization: University of Oklahoma Health Sciences Center To anyone who might be able to help me out a bit: I am currently applying LC/MS to study a complex mixture of purified peptides (peptide sequences vary, as verified by fractionation and Edman sequencing, but average size is known to be 7-10mers). Currently, I have been using a 1.0 x 150mm microbore C18 column, upon which I load 100 micrograms of the peptide mixture and elute with a 30 minute gradient up to 60% acetonitrile (flow rate = 40 microliters/min). While this has proven to be a fairly good and reliable way of "mapping" the peptide mixture in a consistent manner, I would very much prefer to be able to perform the same sort of analysis but with significantly smaller amounts of my peptides (this would be nice, as the whole purification process to actually obtain the mixture is quite labor intensive...this, coupled with the fact that "ironing out" portions of my protocol has required multiple runs, many of which are useless in terms of "real" data). I have been considering "sizing down" and using a smaller column (500, 300, or 180 micron diameter). This is where my question(s) comes in: what could be the potential consequences of using a smaller column: would I be able to obtain the same type of results, or would I be sacrificing either sensitivity or resolution in doing so? If a smaller column would be a valid option for me, then what would be the recommended size to which I should scale down? Thank you for your time; I sincerely appreciate any assistance which you might be able to provide! Kiley R. Prilliman Department of Microbiology & Immunology University of Oklahoma Health Sciences Center phone: 405-271-1203 fax: 405-271-3117 ****************************************************************************** From: John Glowacki Date: 3 Feb 1997 15:59:11 GMT Subject: ICP-MS Aqueous Sn? Organization: UW-Madison I'm looking for some input on running trace level Sn analysis by ICP-MS on surface water samples. Interference equation? Thanks in advance. ****************************************************************************** From: Bill Cotham Date: Mon, 03 Feb 1997 09:24:05 -0500 Subject: Re: GC-MS of pesticides: help on general rules Organization: USC Mass Spectrometry Center Ermanno Errani wrote: } } I am interested in pesticides residues analysis in foods: every time I } find something by GC-ECD or NPD, I try to confirm it by GC-MS with } quadrupole (SIM), in the case I already know the mass spectrum of the } hypotised compound. Otherwise, I must try to get a complete scan (total } ion) for a part of the chromatogram, and watch the scan of each peak } detected (finding Hydrocarbons, waxes, terpenes, acids, lots of naturally } occurring compounds). The library search for matching probability often } gives me very strange kind of molecules with little meaning, and the total } ion scan lack the sensitivity needed for the detection of p.p.m. amounts } of contaminants. I could concentrate 100- to 1000-fold the extract, with } the sure result of great conyamination of liner, column, and even ion } source of quadrupole. } } Is there a rule for monitoring groups of compounds (e.g. fungicides, } phosphoric esters, halogenated compounds) by SIM of few peaks (up to 20) } and with a minimum number of injections ? Thanks for every kind of } suggestions ! } } Ermanno Errani I would first try to clean up your sample matrix (if you are not doing so already). Consult the literature for methods appropriate for the type of samples you are analyzing. -- Bill Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry and Biochemistry University of South Carolina Columbia, SC 29208 803-777-2039 (voice) 803-777-9521 (fax) ****************************************************************************** From: John Butler <72420.1527@CompuServe.COM> Date: 3 Feb 1997 21:39:23 GMT Subject: Re: LC-GC/MS Instrument Organization: TSP The Finnigan SSQ can also be configured for GC or LC. John Butler Thermo Quest ****************************************************************************** From: chthibault@aol.com Date: 4 Feb 1997 14:58:41 GMT Subject: US, NY, Syracuse--Job:Software Engineer, Embedded Systems Organization: AOL http://www.aol.com Leybold Inficon, Inc., located in central New York state, is looking for a Software Engineer responsible for developing and maintaining real-time, embedded software to control the operation of analytical instrumentation, acquire data, and transfer the data to other tasks or processors for subsequent processing. This position reports to the HAPS Software Manager. The successful candidate must be an experienced, self-motivated individual with strong communications and team skills, capable for working with minimal supervision. Minimum educational and work experience is a BS in computer science, electrical engineering, or related technical discipline, with at least 5 years experience or an advanced degree with 3 years experience. Technical experience is required in the following areas: - Real-Time programming including data acquisition, instrument control, and/or signal processing. - Embedded systems programming, including experience developing software where speed, memory and power consumption constraints are critical. - Multi-Task programming, including familiarity with such issues as inter-task communication, multi-task debugging, and parent-child task relations. - Extensive experience in C and some experience in assembly language are required. Experiance in the following areas would be helpful: - Experience with C++ or other object oriented languages would be a plus. - Scientific Programming experience and/or Gas Chromatography and Mass Spectrometry would be a plus. - Experience with various real time operating systems and kernels would be helpful (especially VxWorks). - Experience writing and debugging device drivers would also be helpful. Please send resumes and cover letters, with salary requirements to: Leybold Inficon, Inc. Human Resources, Dept CT 2 Technology Place E. Syracuse NY 13057 ======================================================================== Charlie Thibault Leybold Inficon, Inc ChThibault@aol.com 2 Technology Place (315)434-1268 E. Syracuse NY 13057 ****************************************************************************** From: markusg@hal2000.chemie.uni-mainz.de (Markus Gerle) Date: Tue, 04 Feb 97 21:00:16 GMT Subject: Re: Matrix solutions for MALDI Organization: university of mainz In article <5ctp2a$aul@acmez.gatech.edu>, U61985@UICVM.UIC.EDU wrote: }Can anyone tell me who sell matrix solutions for maldi? I know HP makes }those solutions but I don't have their catolog. Any informations are }welcome. Hello! Why are you interested in such solutions. Normally it is very easy to make the solutions by yourself. You just need the chemicals. For example if you are measuring polystyrene you should use Dithranol as the matrix and a silver-salt (e. g. AgCF3CO2) for enhancement of ionisation. The solvent would be THF. You can buy these chemicals from nearly every chemical supplier. The problem with MALDI is normally that you have to find out the best matrix composition for a specific problem. But I think that there are some people reading this group who can help you if you tell us somethink about the samples you want to measure. Best regards Markus Gerle ****************************************************************************** From: Martin@nezumi.demon.co.uk (Martin Tom Brown) Date: Tue, 04 Feb 97 10:40:55 GMT Subject: Re: ICP-MS Aqueous Sn? Organization: Nezumi In article <5d5lhc$1l@acmex.gatech.edu> jdglowac@facstaff.wisc.edu "John Glowacki" writes: } I'm looking for some input on running trace level Sn analysis by ICP-MS } on surface water samples. Interference equation? } } Thanks in advance. So many isotopes to choose from - one must be interference free. Unless there are high levels of precious metals or tellurium in local surface water Sn118 & Sn120 should be relatively clear. I don't know if Argon trimer causes any trouble though. Regards, -- Martin Brown __ CIS: 71651,470 Scientific Software Consultancy /^,,)__/ ****************************************************************************** From: "Kenneth P. Matuszak" Date: Tue, 04 Feb 1997 16:11:21 -0600 Subject: Reference papers on FC-43 (perfluorotributylamine) Organization: Abbott Labs In the process of validating our mass spec systems, we decided it would be nice to actually have reference papers (preferably refereed journal articles) for each of our chosen mass spec standards. While we have used FC-43 (perfluorotributylamine) in our GC/MS systems for years, we have always referred to tables provided by the FC-43 supplier. Is anyone aware of such papers on FC-43 for positive EI, positive CI, and negative CI? So far we haven't had much success at literature searches (except for some leads on negative CI), although admittedly we could be bungling those. We would be looking for tables of the normally observed ions with masses correct to at least with 0.1 amu. -- Ken M. ****************************************************************** * Ken Matuszak, Ph.D. kenm@cid.pprd.abbott.com * * Abbott Laboratories TEL: (847) 938-9225 * * D418, AP31 FAX: (847) 938-9226 * * 200 Abbott Park Road * * Abbott Park, IL 60064-3537 * ****************************************************************** ****************************************************************************** From: Amish Desai Date: Tue, 04 Feb 1997 14:36:01 -0800 Subject: mass spec future market Organization: CIT Micromachining Group Hello, I am doing a report on mass spectrometry. Specifically, the economics and the future market. I'm trying to find out where I can get some articles or information on this. Any help would be appreciated. I don't normally read/post to this newsgroup so please email. Thank you, Amish ****************************************************************************** From: Christopher Kaine Date: Tue, 04 Feb 1997 20:08:42 -0800 Subject: Re: LC-GC/MS Instrument Organization: OneNet Communications HUB News Server David Sparkman wrote: } } On Tuesday January 28 Witold Winnik, Ph.D. wrote looking for an instrument } that will do EI/CI GC/MS and can be switched to ESI LC/MS. One of the } best instruments for this is the Finnigan/ATI Automass built in Paris. } This instrument was desiged to work along these lines. It is currently } being sold by Finnigan out of Austin, Texas. } } O. David Sparkman } Consultant-At-Large } } Phone: 1-510-754-5003 I believe the individual was looking for a MS/MS instrument. If that is the case, then really the Finnigan TSQ7000, Sciex or the Micromass Quattro fit the bill. Christopher J. Kaine ****************************************************************************** From: Chris Elhardt Date: 5 Feb 1997 13:57:19 GMT Subject: Re: LDV - schematics ?? Organization: Texas Department of Health Contact National Instruments, Austin, TX. cElhardt yamx0001@gold.tc.umn.edu wrote: }I am a physics student at the U of MN. }I'm developing an experiment and need to implement a }Laser-Doppler Velocitometer (LDV), in order to measure }the velocity of Couette-Taylor fluid flow. I only need to measure }1 dimension, and don't need anything fancy of sophisticated. } }Can anyone help me out with schematics for transmitting and }receiving circuits, and interfacing with a PC? } }I have lasers. I just don't know how to design the circuits. } }Please post or email me would be better. } }Thanks. Patrick yamx0001@gold.tc.umn.edu } } ****************************************************************************** From: "Achim Recktenwald, PhD" Date: Wed, 05 Feb 1997 12:39:45 -0500 Subject: Re: Matrix solutions for MALDI Organization: IBEX Technologies, Inc., Biochemistry, 5485 Pare, Montreal, PQ, H4P 1P7, Canada Markus Gerle wrote: } } In article <5ctp2a$aul@acmez.gatech.edu>, U61985@UICVM.UIC.EDU wrote: } }Can anyone tell me who sell matrix solutions for maldi? I know HP makes } }those solutions but I don't have their catolog. Any informations are } }welcome. } } Hello! } } Why are you interested in such solutions. Normally it is very easy to make } the solutions by yourself. You just need the chemicals. For example if you } are measuring polystyrene you should use Dithranol as the matrix and a } silver-salt (e. g. AgCF3CO2) for enhancement of ionisation. The solvent } would be THF. You can buy these chemicals from nearly every chemical } supplier. The problem with MALDI is normally that you have to find out } the best matrix composition for a specific problem. But I think that there } are some people reading this group who can help you if you tell us } somethink about the samples you want to measure. } } Best regards } Markus Gerle For lots of stuff for MALDI check out: http://chait-sgi.rockefeller.edu/ HP's MALDI-stuff you'll find at: http://www-dmo.external.hp.com:80/apg/main.html Achim ****************************************************************************** From: "Richard B. van Breemen" Date: Wed, 5 Feb 1997 16:33:17 -0600 (CST) Subject: Finnigan MAT 90 Organization: * FOR SALE: Finnigan MAT 90 reversed geometry magnetic sector mass spectrometer equipped with FAB, EI, CI, DCI and a Varian 3400 GC for GC-MS. This instrument has been well maintained and is currently the workhorse high resolution, FAB and GC-MS instrument on campus. However, it will be replaced by a newer model. The MAT 90 is available immediately. Please contact either of us for more details and to make your best offer: Richard B. van Breemen University of Illinois at Chicago Department of Medicinal Chemistry and Pharmacognosy 833 S. Wood St. Chicago, IL 60612-7231 email: richard.vanbreemen@uicvm.uic.edu Telephone: (312) 996-9353 FAX: (312) 996-7107 or Duane L. Venton University of Illinois at Chicago Department of Medicinal Chemistry & Pharmacognosy 833 S. Wood St. Chicago, IL 60612-7231 email: venton@uicvm.uic.edu Telephone: (312) 996-5233 FAX: (312) 996-7107 ****************************************************************************** From: Philip Berger Date: Thu, 06 Feb 1997 05:49:35 GMT Subject: Re: Teknivent Corp. [1/1] Organization: Netcom In response to numerous inquiries in this news group, I felt that an announcement other than those already released would be in order. On December 10, Teknivent Corp. sold its assets to ProLab Resources, Inc. of Madison, WI. During its 20+ years of service to the international mass spectrometry community, Teknivent automated over 1000 GC/LC-MS instruments manufactured by nearly every commercial vendor here and abroad. We had the pleasure of working with such pioneering organizations as Extrel, Vestec, Hitachi, Finnigan and a host of other manufacturers as a contractor or OEM supplier. As our products were often the only ones that could automate many of the older instruments still actively in use, they offered a new lease on life to these high-cost investments, greatly extending their useful life cycle. Our Vector(TM) Series data systems (Vector/One and Vector/Two) introduced in 1983 and 1990, respectively, set the standard in PC-based MS automation, with free software upgrades for the life of these units. More recently, our Envirolink(TM) data system has become the most popular retrofit for environmental GC/MS instruments. As a young entrepreneur in 1976, I believed that computers (mini’s, in those days!) could be cost-effectively produced that would greatly simplify the work of professional mass spectrometrists and routine operators by providing far better analyses with less operator fatigue. Through our three product generations, we were fortunate to be able to work with many of the leading chemists in this field and will always cherish their ideas, energy and enthusiasm in "test driving" our earliest--and often clumsiest--offerings. To the vendors with whom we worked in customizing our designs to their wide-ranging instrument and application requirements, I am particularly indebted for your faith in the talents of our small working group and its ability to provide innovative, economical solutions that allowed your companies to quickly move into target markets with fully automated products. As I leave Teknivent to pursue other dreams, I want to thank all of our customers for their many years of loyal patronage and for their thousands of hours of interactive discussions that helped shape our company'’s direction, its products, and most importantly, its culture. To Keith Martinko, founder and CEO of ProLab Resources, Inc.(http://www.prolab.com), I wish you and your colleagues every good fortune as you inherit the legacy we established together. Best wishes for a healthy and happy new year. Philip S. Berger President and CEO ****************************************************************************** From: E.A.Underwood@exeter.ac.uk Date: Thu, 6 Feb 1997 14:50:46 +0000 (GMT) Subject: HANDS ON MASS SPEC Organization: * Dear All, I have been asked to find out availability and prices of mass spectrometers that might be suitable for hands on use by post graduate students and undergraduates who are working on research projects as part of their degree course. The department mass spec service is run on a Kratos Profile mass spectrometer, capable of low and high resolution in EI, CI, and LSIMS modes. The system has a GC interface and a solids probe, and must be shut down to change over to LSIMS. Masses up 2400 amu are routinely obtainable. Our case for getting a share of the limited funds available would be improved if, as well as being capable of routine work, we could extend the range of mass spec facilities in the department with a single purchase. I would very much appreciate hearing from anyone with experience of hands on mass spectrometers, or suggestions as to which instrument manufacturers we might approach. This request has been mailed to other relevant lists, and I apologise to those of you who receive duplicates. Thank you all in advance for your help, Eric Underwood. Mr Eric Underwood Tel 01392-263438 Spectroscopic Services Department of Chemistry University of Exeter Fax 01392-263434 Stocker Road Exeter EX4 4QD E-mail E.A.Underwood@exeter.ac.uk "Everything not compulsory is forbidden" ****************************************************************************** From: John Subject: HELP! HP MS Chemstation Pascal software Date: Wed, 05 Feb 1997 21:51:26 GMT HP 5890A + 5970 MSD with 9000/300 computer and 9153B hard/floppy The hard drive has died on our 9153B and we no longer have the original disks. Can anybody help with a copy of v3.1 or 3.2? HP aren't interested and can't supply. Any help with sourcing a replacement 20Mb drive would also be appreciated! Hope somebody can help. John email: johncleg@epichem.co.uk ****************************************************************************** From: (David Bostwick) Date: Fri, 7 Feb 1997 00:13:27 -0500 (EST) Subject: Power outage Organization: * Most computer systems at Georgia Tech will be unavailable from 0400 EST Saturday, February 8, until 0700 EST Tuesday, February 11 to complete the installation and testing of several UPSes. This outage will include the machine from which sci.techniques.mass-spec is moderated. No new messages will be approved, and e-mail will not be accepted. Most UNIX mailers will continue sending messages for up to 5 days, and will give a warning message to the sender. Other mailers may simply report the failure and will not try to send the message again. We regret the disruption. We will check the system regularly, and will begin approving messages again as soon as possible. -- David E. Bostwick Georgia Institute of Technology, Atlanta, GA, 30332 david.bostwick@chemistry.gatech.edu ****************************************************************************** From: lcpo@unice.fr (J.-F. Gal) Date: Tue, 11 Feb 1997 08:21:16 +0000 Subject: Reference papers on FC-43 (perfluorotributylamine) Organization: * To the attention : "Kenneth P. Matuszak" Date: Tue, 04 Feb 1997 16:11:21 -0600 Subject: Reference papers on FC-43 (perfluorotributylamine) Organization: Abbott Labs It may help to read : "Chemical mass markers in CI-MS", D.V. Bowen, F.H. Field; Anal. Chem. 1975;47(13); 2289-2292 -(n-C4H9)3N is discussed. I didn't find a ref to the EI ms in this paper anyway. Jean-Francois Gal ****************************************************************************** From: "Greg R. Shaw" Date: Mon, 10 Feb 1997 13:12:07 -0800 Subject: GC/MS Position Available Organization: West Coast Analytical Service Job Title: GCMS Operator Job Description: West Coast Analytical Service, Inc. (WCAS) is looking for chemists that are experienced GCMS operators. This experience must include work on environmental samples for volatiles and semivolatiles as well as screens of unknown materials. Experience with pharmaceuticals and air samples would be an advantage. Must have a minimum of a B.S. degree, over 5 years experience, able to solve problems, repair instruments, only interested in high quality work, can work under strict deadlines, and has excellent organizational skills. Please Contact Eric Lindsay eric.lindsay@wcaslab.com 9840 Alburtis Ave Santa Fe Springs, CA 90670 Voice (562)948-2225 Fax (562)948-5850 -- Greg R. Shaw West Coast Analytical Service greg.shaw@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: eri@mindlink.bc.ca (Elemental Research Inc.) Date: Mon, 10 Feb 1997 19:48:55 GMT Subject: VG PQ-I Spare parts available Organization: MIND LINK! - British Columbia, Canada Some months ago I posted a list of spare parts we have available for a VG PQ-I ICP-MS (ours finally died). We sold many of them, but still have a few left. Just in case anyone missed the first post, this is what we have left: A. Working Units ------------- Lens supply (s/n 1894-05) $700.00 Lens supply (s/n 1993-03) $700.00 Power control (s/n 3001) $ 450.00 RF coupler $ 250.00 B. Main power board PCB's: ----------------------- MR504-200C3 issue 2 (1) $ 100.00 each MR504-204C3 issue 3 (1) $ 100.00 each MR504-207C3 issue 1 (2) $ 100.00 each MR504-202C2 issue 5 (2) $ 100.00 each MR504-205C3 issue 3 (2) $ 100.00 each MR504-201C3 issue 2 (4) $ 100.00 each MR504-203C3 issue 2 (2) $ 100.00 each MR504-2013C3 issue 1 (2) $ 100.00 each C. Quad Controller Boards ------------------------- MA 3020-200 P/D (1) $100.00 MA 495-69C (1) $100.00 MA 495-70C (1) $100.00 MA 495-63C (1) $100.00 MA 495-65C (1) $100.00 MA 495-76C (1) $100.00 MA 485-62C (1) $100.00 D. Instrument controller boards ------------------------------- PACTROL BOARD (2) $ 100.00 each E. Other -------- Edwards vacuum controller Model 1105 $1500.00 Gilson 222 autosampler $4000.00 Quad RF generator, valve type $1000.00 Lense stack $4000.00 (all prices in $US) 20% off the above prices if you buy a large part of the above. Cheers, Ray Ford Elemental Research Inc. 309-267 West Esplanade North Vancouver, BC V7M 1A5 Canada (604) 986-0445 (604) 986-0071 eri@mindlink.bc.ca ****************************************************************************** From: Tomasz Gibinski Date: Mon, 10 Feb 1997 13:01:13 +0100 Subject: Detection fullerenes in MS Organization: Adam Mickiewicz University Which metod is the most propriet for detection of C60 ? gibi@chem.amu.edu.pl ****************************************************************************** From: nathand@cco.caltech.edu (Nathan Dalleska) Date: 11 Feb 1997 00:39:29 GMT Subject: Re: LC-GC/MS Instrument Organization: California Institute of Technology If you can afford to shell out the big bucks and happen to need high resolution (or can use HR to solve some of your problems) Micromass' ProSpec is a chromatography workhorse. A FFR gas cell and linked scans will do decent MS/MS. -Nathan Dalleska -- Nathan F. Dalleska, Ph.D. CCE Mass Spectrometry Laboratory California Institute of Technology138-78 Pasadena, CA 91125 818.395.6533 nathand@cco.caltech.edu ****************************************************************************** From: vanreet@ilink.nis.za (Teunis vanRee) Date: 9 Feb 1997 13:41:52 GMT Subject: Re: Looking for nice GCMS demo Organization: Network Information Services We expect delivery of our new GCQ any moment now, and are planning a 'commissioning' function to show the donors and management where the money went. We would like to demonstrate the instrument with some simple but interesting (to laymen) separation & identification. Any suggestions would be more than welcome. Thanks Thomas ****************************************************************************** From: Keith Martinko Date: Tue, 11 Feb 1997 11:34:36 -0600 Subject: Teknivent Corp. purchased by ProLab Resources, Inc. Organization: ProLab Resources, Inc. To all members of the mass spec community : ProLab Resources, an innovative software and hardware development firm headquartered in Madison, WI, is proud to announce the acquisition of Teknivent Corp. and it's Vector/Two and EnviroLink PC-based mass spectrometry data system products. As a former Vice President of Product Development at Teknivent Corp. for over seven years, I am excited to once again lead the effort within ProLab Resources to improve this unique technology to better serve your needs and the needs of the analytical laboratory marketplace. During this year, we plan to invest heavily in developing new features for both product lines and expanding the base of mass spectrometers supported by this technology. For up-to-date information, please visit us anytime at http://www.prolab.com. ProLab Resources' realizes that one of the greatest assets behind the Teknivent product line is it's large base of talented and dedicated customers. If you are a Teknivent Owner, your ideas have helped to significantly improve the performance of both the Vector/Two and EnviroLink data systems. Although Teknivent is no longer available to service your existing products, ProLab Resources is available to provide continued support for your Teknivent systems. To aid in providing support continuity for your laboratory, ProLab Resources has retained the technical services of both Lindsay McAnulty and Bill Bertrand, previously Senior Support Engineers at Teknivent Corp.. Their intimate knowledge of your laboratory configuration and specific application needs will help ProLab Resources to quickly provide the highest level of support for your systems. Finally, I would like to personally thank all Teknivent customers for your past support of Teknivent and its products, and all of us at ProLab Resources look forward to establishing a new relationship with your organization. We look forward to hearing from you soon and please feel free to email me at prolab@ix.netcom.com or call me at 608-278-0002 if you have any additional questions. Best regards, Keith Martinko President ProLab Resources, Inc. prolab@ix.netcom.com http://www.prolab.com ****************************************************************************** From: "Greg R. Shaw" Date: Tue, 11 Feb 1997 11:50:23 -0800 Subject: Finnigan 4500 for Sale Organization: West Coast Analytical Service For sale: Finnigan 4500 quadrupole GC/MS/DS. 2-1024 mass range, 9610 GC, dual diffusion pump, solids probe, -3kV dynode, Incos Rev 6.5 software (including 42000 entry NIST library), Printronix MVP printer, 70 Mb Winchester drive, Cipher streamer tape. Includes user and service manuals, full schematics. Please Contact: eric.lindsay@wcaslab.com -- Eric Lindsay West Coast Analytical Service eric.lindsay@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: PAREESDM@apci.com Date: Tue, 11 Feb 1997 18:01:26 -0500 Subject: response to Winnik and Cotham Organization: * Witold Winnik, Ph.D. wrote: } } Hello, } } I am trying to find a mass spectrometer which can perform the following tasks: } 1. reasonably easily switches between GC/MS and LC-electrospray jobs (one } week it would perform a GC/MS analysis and following weeks LC/MS, for } example) } 2. capable of EI / CI and positive / negative ionization } 3. performs MS and MS/MS analysis; mass range up to 3000-4000 m/z } And costs below $400,000. } } I know that Finnigan GCQ and LCQ ion traps would qualify jointly but the } problem is I can request only one instrument in my proposal, even though it } would be probably less expensive to acquire both GCQ and LCQ instead of one } instrument that can switch between GC and LC jobs. } If anyone knows about such instrument, or has been using it please contact } me at: winnik@charlotte.med.nyu.edu } } Witold Winnik } NYU Medical Center And Bill Cotham responded: }I think a Finnigan TSQ or a Micromass Quatro would meet all of your }specs. Well, I have one of each of these instruments in my lab. I can't speak to the issue of doing electrospray on the 7000 but it does excellent EI and CI. However, while our electrospray on the Quattro has been problematic the EI and CI was dismal. In fact it was the incredibly poor performance of this system in EI and CI modes that caused us to purchase the 7000. The EI spectra were frequently biased severely toward low mass ions. For example, sometimes the base ion for toluene would be at m/z = 39. This was not a tuning issue. CI was also a problem, with significant fragmentation that we've never seen on other instruments. It is possible that Micromass has redesigned the source and these problems have been solved, but I'd certainly scrutinize this closely before making a decision. Dave Parees pareesdm@apci.com my comments are not associated with my employer ****************************************************************************** From: "Karl J. Treier" Date: 12 Feb 1997 01:48:06 GMT Subject: ITS40, Magnum & GCQ Users ! Organization: UniTech Attention ITS40, Magnum and GCQ GCMS Users, now available... MS2TXT Convert .MS datafiles into delimited ASCII files for easy import into Spreadsheet or Database software, available in Windows 3.1 and 95/NT versions for $249 + Shipping. MS2HP Convert .MS datafiles into HP Chemstation (.D) format, available in Windows 3.1 and 95/NT versions for $249 + Shipping. SPL2BMP Convert .SPL Spooler files into individual .BMP graphics files for easy import into graphics programs, available in Windows 3.1 and 95/NT versions for $149 + Shipping. Finally it's really available, sorry for the interminable delays. The two volume Procedure Language Reference Manual Set, almost 1000 pages of invaluable documentation for $495 + Shipping. -- UniTech 680 Northland Boulevard, Suite 9 Cincinnati OH 45240, USA Phone: 1-800-989-0144 or (513) 825-0144 Fax: (513) 825-0163 e-mail: info@uni-tech.com web site: http://www.uni-tech.com ****************************************************************************** From: julie_parker@aq.ehnr.state.nc.us Date: Tue, 11 Feb 1997 14:01:46 -0600 Subject: GC/MS Calculation of concentrations via ISTD Organization: * I need information regarding the actual calculation of compound concentration within a sample. I will be using an Internal standard for all unknown samples. Forgive me if this is a 'not so intelligent' question but should the calculations, using response factor and sample amounts, be based on the chromatographic peak areas or the mass spec abundance values. I would appreciate any help or info. These are air samples and an internal standard of BFB. Once again...thanks! Julie ****************************************************************************** From: Charles Metcalfe Date: Wed, 12 Feb 1997 13:48:55 -0500 Subject: GC/MS - Pyrolysis Products of Fluoroacrylic Resins Organization: Voicenet - Internet Access - (215)674-9290 I have built a small database of fluoroacrylic resins used to waterproof fabric and leather. Since these types of compounds are proprietary, I use this technique to identify the resin in consumer products. I would like to be able to attach some structural information to the pyrolysis fragments. Any information out there? ****************************************************************************** From: PAREESDM@apci.com Date: Wed, 12 Feb 1997 16:18:38 -0500 Subject: response to question about demo samples Organization: * The following question was posted: }From: vanreet@ilink.nis.za (Teunis vanRee) }Date: 9 Feb 1997 13:41:52 GMT }Subject: Re: Looking for nice GCMS demo }Organization: Network Information Services }We expect delivery of our new GCQ any moment now, and are planning a }'commissioning' function to show the donors and management where the money }went. We would like to demonstrate the instrument with some simple but }interesting (to laymen) separation & identification. Any suggestions }would be more than welcome. }Thanks }Thomas Over the years we have given many demos to managers, customers of our company and students from high schools and colleges. Samples that work very nicely include gasolines and perfumes. These provide nice but complex chromatograms, with peaks that behave well chromatographically. Many of the compounds will be in standard data bases to demonstrate the use of "library" matching and show what is in these materials. Ion mapping is also impressive because these complex mixtures will contain a number of unresolved chromatographic clusters, and you can demonstrate the power of mass spectrometry to deconvolute spectra in such situations and identify individual species. This can be done by deconvoluting the spectra first or taking the mixed spectrum to the library, subtracting the spectrum of a matched component, and then searching the remainder spectrum. Generally, these materials only have volatile compounds and the analyses can be completed in 20-30 minutes for such a demonstration, although this would not provide optimal separation of the greatest number of components (such analyses of these materials take 1-2 hours). If this is too long, you could consider a liquor, such as a whiskey. There are far fewer compounds and with the right choice of conditions, an analysis can be completed in 7-15 minutes. Analyzing different gasolines (premium vs. regular) or perfumes or liquors (either different types [vodka, rum, scotch] or different brands of the same liquor [expensive vs. cheap scotch can be very enlightening] can be used to show the variation in composition. Many chromatographic supply companies, such as Supelco, J&W, Chrompack or Restek, to name a few, have numerous examples of many of these types of materials, giving you ideas of chromatographic conditions and the compositions that you will see. Dave Parees My ideas are not the responsibility of my company ****************************************************************************** From: Tra@uunet.uu.net, Huu@uqam.ca (Dr. Huu van Tra) Date: 12 Feb 1997 21:31:05 GMT Subject: WANGCO Disk Drive Organization: UQAM I'm looking for one or two used (but in working conditions) WANGCO Disk Drive(s) for a NOVA-3 Data System. This data system is used for collecting data and control the FINNIGAN Mass Spectrometer Model 4000. If anyone wants to dispose them, please contact: Prof. Huu van Tra Dept. of Chemistry Universite du Quebec a Montreal Montreal (Qc) Canada E-Mail: TRA.HUU@UQAM.CA ****************************************************************************** From: PAREESDM@apci.com Date: Wed, 12 Feb 1997 16:40:36 -0500 Subject: GC-MS calculation of concentrations Organization: * The following query was recently posted: }From: julie_parker@aq.ehnr.state.nc.us }Date: Tue, 11 Feb 1997 14:01:46 -0600 }Subject: GC/MS Calculation of concentrations via ISTD }Organization: * }I need information regarding the actual calculation of compound }concentration within a sample. I will be using an Internal standard for }all unknown samples. Forgive me if this is a 'not so intelligent' question }but should the calculations, using response factor and sample amounts, be }based on the chromatographic peak areas or the mass spec abundance values. }I would appreciate any help or info. These are air samples and an internal }standard of BFB. Once again...thanks! }Julie When doing this type of analysis on a GC-MS as compared to a GC-FID or some other type of "single signal" detector, the chromatographic peak areas are a summation of the individual MS ion abundances for the ions in the species present that fall within the mass range scanned in the analysis. Therefore, you have the choice of using any combination of ions that makes the most sense for doing your quantitation. If there is full chromatographic separation, there is no reason to pick specific ions, but then a mass spectrometer is not necessarily needed in the first place (other than as proof that there are, in fact, no co-eluting interfering species present). Usually, a GC-MS method will involve the choice of a specific ion for quantitation, one or two check ions (see below), and a retention time window as further proof of identity. If complexity of the sample necessitates MS, then it makes sense to calculate response factors for one (better yet, 2-3) ions in the spectrum of the species of interest. These should be as high in mass as practical (better selectivity, generally) as long as the abundances are great enough to provide adequate detectibility and the chosen ions are not also found in any expected co-eluting compounds. If the concentration range is low enough, selective ion monitoring may be appropriate or necessary. This decreases the detection limits, but means that you now have less spectral information, making it very important that you understand the matrices of your samples, possible interferences, etc. You could still choose to calibrate and quantitate on the individual ions or the sum of those monitored. Generally, the analyst will choose to calibrate the relative response of each ion individually and will compare the quantitative results from more than one ion as added verification of the quality of the result and absence of interferences. Dave Parees My employer is not responsible for my opinions ****************************************************************************** From: gs_klinger@pnl.gov (George S Klinger) Date: Wed, 12 Feb 1997 22:49:13 +0000 (GMT) Subject: Re: GC/MS Calculation of concentrations via ISTD Organization: Battelle Pacific Northwest National Labs (PN2L) In article <5drelq$sm3@acmey.gatech.edu>, julie_parker@aq.ehnr.state.nc.us says... } }I need information regarding the actual calculation of compound }concentration within a sample. I will be using an Internal standard for }all unknown samples. Forgive me if this is a 'not so intelligent' question }but should the calculations, using response factor and sample amounts, be }based on the chromatographic peak areas or the mass spec abundance values. }I would appreciate any help or info. These are air samples and an internal }standard of BFB. Once again...thanks! }Julie } } You need to do some very basic reading about the basics of instrumental methods and analytical chemistry. But here's an oversimplied anwser to your question: integration statistics are best for a properly sampled peak, say 20 points. Seven points is really a minimum, but considered a good compromise full scan for GC/MS work. "mass spec abundance" sounds like a single point to me - a crude peak height if you will. You decide. George ****************************************************************************** From: Don Magin Date: Thu, 13 Feb 1997 11:11:46 -0500 Subject: LCMS courses Organization: Whitehall Robins Does anyone know of any courses (not meetings or conferences) which will be held during 1997 in the US or Canada which would focus on either particle beam; analysis of pharmaceuticals, foods, or cosmetics; or adapting analytical LC methods to LCMS conditions. My particular application for LCMS is to identify complete unknowns which might appear during HPLC analysis of over-the-counter pharmaceuticals which have been intentionally stressed by heat, light, oxygen, etc. Thanks for any info. Don Magin ****************************************************************************** From: "Ren\ BOUTIN" Date: Thu, 13 Feb 1997 18:56:50 +0100 Subject: ICP MS comparison Organization: LCAN Universit de La Rochelle Hello, I search some critical informations on different ICPMS systems. Please, I need of your experiences on this sytems. Thank You Rene ****************************************************************************** From: dwheeler@nu-ins.u-net.com (Dave) Date: Wed, 05 Feb 1997 03:11:10 GMT Subject: Re: ICP MS comparison Organization: U-NET Ltd It would help if you gave us some more information. What do you need to know? Is this an instrument performance or application question? Is your interest in quadrupole or sector based instruments? Regards, Dave Wheeler On 13 Feb 1997 13:12:19 -0500, "Ren\ BOUTIN" wrote: }Hello, } }I search some critical informations on different ICPMS systems. } } }Please, I need of your experiences on this sytems. } }Thank You } }Rene } } } } ****************************************************************************** From: software@squirrel.com.au (software) Date: 13 Feb 1997 23:01:17 GMT Subject: Silicon Graphics / Sun Workstation Giveaway Organization: Squirrel Internet, Brisbane, Australia Silicon Graphics and SUN workstations are renowned for their blinding speed and 3D capabilities - the same qualities that describe the latest 1997 breed of websites. Think of web sites as being more interactive, faster and compelling with Java technology. It costs nothing to have a look and you could be part of our Workstation giveaway - and keep up with the latest in web page design. Surf to.. Web based Shopping Mall System: http://www.squirrel.com.au/shop Web site Menu System: http://www.squirrel.com.au/nuts Your comments are appreciated. Here are some so far.. "WOW!! This is fun and fast!" "Best use of Java Technology I've seen." "Improved my web site 10 times." "Boring - NOT! Forget fat animated gifs, this is better!" We also hope you've had a great holiday period and we wish you all the best for the new year. The Squirrel Software Team ;) "*^~-..,,_,,..-~^*"*^~-..,,_,,..-~^*"*^~-..,,_,,..-~^*"*^~-..,,_,,..-~^* _ _ |\__/| .~ ~. Give me speed /o=o'`./ .' {o__, \ { and make it cool! / . . ) \ `-` '-' \ } .( _( )_.' '---.~_ _ _| _,,..-~^*"*^~-..,,_,,..-~^*"*^~-..,,_,,..-~^*"*^~-..,,_,,..-~^*"*^~-..,,_ http://www.squirrel.com.au ****************************************************************************** From: pvang@sci.kun.nl (Peter van Galen) Date: 14 Feb 1997 08:18:57 +0100 Subject: GC/MS determination of thiophenes? Organization: University of Nijmegen, The Netherlands Summary: How too determine the identity of low molecular weight sulfur containin Hello out there, I was asked wether it is possible too determine the compostion of a gas mixture containing compounds as CH3SH, C2H5SH, COS, CS2, CH3S-SCH3 and possibly related compounds. I've given it a shot at a Saturn II equipped with a HP-1 column, knowing that there was hardly any chance for succes, but just too see if some evidence for the contents of sulfur could be found. The only thing achieved was some circumstantial evidence for CH3S fragmentation. I would like too know what the best column for this purpose is. And in addition as it is likely to be a one shot experiment, who, within reasonable distance, could do such an experiment for us. Thanks in advance, |___________________________ Peter M. van Galen |____ Mass Spectrometry, Organic Chemistry Dept. |__________________ Nijmegen University |_ Toernooiveld 1, 6525 ED Nijmegen, Netherlands |___ FAX 024-3652929, E-mail pvang@sci.kun.nl ****************************************************************************** From: "Ren\ BOUTIN" Date: Fri, 14 Feb 1997 11:48:35 +0100 Subject: Re: ICP MS comparison Organization: LCAN Universit de La Rochelle My interest is focused on the performances of the quadripole based systems. Rene ****************************************************************************** From: James Pitt Date: Fri, 14 Feb 1997 22:12:45 +1000 Subject: Statistical comparison of GC-MS profiles Organization: CSIRO Can anyone help me with this problem: I want to statistically compare sets of GC-MS data (eg 10x GC-MS profiles from a study group compared to 10x profiles from controls) in order to identify significant compounds. The data need to be compared on a scan by scan, m/z by m/z basis. The resulting stats could then be put into a data set very similar to a GC-MS data set and "chromatograms" or "spectra" of statistical significance could then be extracted. I can think of ways in which this could be programmed but I feel that I would probably be re-inventing the wheel. Does anyone know of any programs (peferably ones that handle HP data files)that could do this sort of thing? Thanks for any answers. ****************************************************************************** From: "OCAS" Date: 14 Feb 1997 13:01:58 GMT Subject: laser-probe on PQII Organization: OCAS We are trying to install a laser probe on our PQ II . The z stage is at the maximum and if we put the flash energy to 750 Voltage (like described in the manuel) we distroy our laser cell. We work with the low magnitude lens and the biggest laser cell . Can anyone help us out. P.S. we also are looking for the reference number to buy a new big laser cell. We already asked Thermogroup Holland but they can't help us. Cathy ****************************************************************************** From: pggreen@cco.caltech.edu (Peter G. Green) Date: Fri, 14 Feb 97 16:23:36 GMT Subject: Re: ICP MS comparison Organization: Caltech (Environmental Engineering Science) }I search some critical informations on different ICPMS systems. } }Please, I need of your experiences on this sytems. } Start from http://www.geochemistry.syr.edu/cheatham/icpmsins.html for information on, and links to, research groups that use ICP-MS, manufacturers of them and their accessories, etc... ****************************************************************************** From: pggreen@cco.caltech.edu (Peter G. Green) Date: Fri, 14 Feb 97 16:28:08 GMT Subject: Re: LCMS courses Organization: Caltech (Environmental Engineering Science) }Does anyone know of any courses (not meetings or conferences) which will }be held during 1997 in the US or Canada which would focus on either }particle beam; analysis of pharmaceuticals, foods, or cosmetics; or }adapting analytical LC methods to LCMS conditions. ... } }Thanks for any info. Hewlett-Packard holds a variety course around North America every year. Call 800-227-9770 for info, free catalog. The ASMS happens to have an LC/MS course focussing on ES and APCI (including mention of your application) in Palm Springs, CA this May 31-June 1. They're at http://www.asms.org/welcome.html -Peter (regular customer of the former, former member of the latter) ****************************************************************************** From: Brett Phinney Date: Fri, 14 Feb 1997 13:40:45 -0800 Subject: peptide mass calculations Organization: University of Texas at Austin I am doing a peptide map of a protein I am interested in. For some preliminary data I am first doing a trypsin digest and then I am using a mass spec. to get the mass of my fragments, with the hope that I will be able to determine disulfide bond locations. My question is that when I calculate the expected mass of my fragments I can get differing results if I calculate the mass using the average mass or the monoisotropic mass. Does anyone have any suggestions as to which calculation method I should use? Does anyone have a good reference on this subject? Thanks a lot Brett Phinney ****************************************************************************** From: HK WANG Date: Fri, 14 Feb 1997 22:25:58 -0500 Subject: About VG 7070E GC/MS Organization: UNC-Chapel Hill Dear GC/MS experts: I am new to this newgroup. Please foregive me if my questions are not new to you. I will in charge of VG7070E Mass spectrometer for my department. Two Questions here: 1) I am going to order gases (argon, xenon, and helium) and regulators for the instrument. What grade is necessary? High purity or ordinary? 2) Current computer system came with this used VG7070E is DEC PDP11/24 minicomputer with VGII/250 software? What kind upgrade options are available today either hardware or software? How much cost if you can give quote? Thanks for your attention. HK WANG, Ph.D. Research Associate Professor Room 302, Beard Hall School of Pharmacy Univ. of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7360 Tel: 919-966-5908 FAX: 919-966-3893 Email: hwang@gibbs.oit.unc.edu ****************************************************************************** From: debayfpk@ibmmail.com Date: Sat, 15 Feb 1997 03:13:51 EST Subject: Witold Winnik: Instrument for EI/CI-MS and ESI-MS Organization: * Witold Winnik, Ph.D. wrote: } } Hello, } } I am trying to find a mass spectrometer which can perform the following tasks: } 1. reasonably easily switches between GC/MS and LC-electrospray jobs (one } week it would perform a GC/MS analysis and following weeks LC/MS, for } example) } 2. capable of EI / CI and positive / negative ionization } 3. performs MS and MS/MS analysis; mass range up to 3000-4000 m/z } And costs below $400,000. } } I know that Finnigan GCQ and LCQ ion traps would qualify jointly but the } problem is I can request only one instrument in my proposal, even though it } would be probably less expensive to acquire both GCQ and LCQ instead of one } instrument that can switch between GC and LC jobs. } If anyone knows about such instrument, or has been using it please contact } me at: winnik@charlotte.med.nyu.edu } } Witold Winnik } NYU Medical Center And Dieter Zimmer responded: Hello, Somebody gave you the advice to by a SCIEX instrument. This is a really excellent instrument for LC-ESI-MS/MS and LC-APCI -MS/MS (we have 3 pieces and expect the 4th soon!), but it is dedicated to do these jobs exclusively, that is, due to the construction of the SCIEX instruments neither EI nor CI (and GC/MS) are possible!!! However, in the past I made some instrument evaluations and with this experience I would recommend to by the Finnigan TSQ 7000, as some other people recommended to you before. I'm sure that this instrument will fully meet your specifications and demands regarding EI/CI, LC/MS using ESI and APCI and sensitive MS/MS either in psoitive as well as in negative ion mode. Dieter Zimmer My ideas are not the responsibility of my company ****************************************************************************** From: vanreet@ilink.nis.za (Teunis vanRee) Date: 15 Feb 1997 08:19:19 GMT Subject: Re: peptide mass calculations Organization: Network Information Services Brett Phinney (brettsp@mail.utexas.edu) wrote: : I am doing a peptide map of a protein I am interested in. For some : preliminary data I am first doing a trypsin digest and then I am using a : mass spec. to get the mass of my fragments, with the hope that I will be : able to determine disulfide bond locations. My question is that when I : calculate the expected mass of my fragments I can get differing results : if I calculate the mass using the average mass or the monoisotropic : mass. Does anyone have any suggestions as to which calculation method I : should use? Does anyone have a good reference on this subject? There's no way you can use the average mass no. The mass spectrometer sees only monoisotopic ions. So calculate the monoisotopic mass nos. Regards ****************************************************************************** From: lcpo@unice.fr (J.-F. Gal) Date: Sat, 15 Feb 1997 10:34:47 +0000 Subject: gas analysis of sulfur contaning compounds Organization: * |___________________________ Peter M. van Galen |____ Mass Spectrometry, Organic Chemistry Dept. |__________________ Nijmegen University |_ Toernooiveld 1, 6525 ED Nijmegen, wrote : wrote: }We are trying to install a laser probe on our PQ II . } }The z stage is at the maximum and if we put the flash energy to 750 Voltage }(like described in the manuel) we distroy our laser cell. }We work with the low magnitude lens and the biggest laser cell . }Can anyone help us out. } Hi You need to maximise the energy density on the sample while minimising the energy density on the cell window. To do this increase the separation between the top of the sample and the cell window. If you have the sample too close to the cell window ablation of the cell is bound to occur. You mention that your Z travel is at a maximum. Have you raised the sample within the sample cell too far to allow laser focus to be achieved? If so I would think that a spacer is missing from your LaserProbe. It should be possible to focus the laser on the sample without having the sample surface too close to the sample cell window. Also, do you need to use 750V? It may be that you have a particularly powerful laser and ablation of the sample (and not of the cell) may be possible at lower flash lamp voltages. I would check this with VG Elemental. }P.S. we also are looking for the reference number to buy a new big laser }cell. We already asked Thermogroup Holland but they can't help us. } }Cathy Also I would suggest that you contact VG Elemental for this part number. I don't have their customer care email address, but I am sure that Paul Sigsworth at Elemental will be able to supply this. His email is paul.sigsworth@vgelemental.com and their web site is at http://www.vgelemental.com/ I hope this helps. Regars, Dave Wheeler email dwheeler@nu-ins.u-net.com ****************************************************************************** From: David Sparkman Date: Sat, 15 Feb 1997 10:19:00 -0500 Subject: LC/MS Applications Courses for PB Organization: * On 13 Feb. 1997 Don Magin of Whithall Robins wrote: }Does anyone know of any courses (not meetings or conferences) which will }Be held during 1997 in the US or Canada which would focus on either }on Particle beam; analysis of pharmaceuticals, foods, or cosmetics; or }adapting analytical LC methods to LCMS conditions. My particular }application for LCMS is to identify complete unknowns which might appear }during HPLC analysis of over-the-counter pharmaceuticals which have been }intentionally stressed by heat, light, oxygen, etc. Peter Green at Caltech replied with the courses offered by HP and ASMS. In addition, the American Chemical Society (ACS) has a number of coureses on LC/MS. Two of these are being taught by Jack Henion: one at the Pittsburgh Conference in Atalanta (March 15-16) and the other with a pharmaceutical ingredients meeting in Philadelphia (April 23-24) Jack also will be offering a course in conjuction with the LC/MS conference being held at Cornell at the end of July. Jack's courses are very good and have some good application's information; however, because of his areas of work, the emphasis is more on APCI and ESI than particle beam. Jack is an outstanding speaker and as one of the pioneers in API LC/MS, he is very knowledgable. I know several people who have taken Jack's courses and they speark very highly of them. The ACS courses are always one of the best attended and are always sold out. I am teaching a course for the ACS at the National meeting in San Francisco (April 14-15) with Fred Klink. This course will have a good amount of particle beam applications, but it, like Jack's course, is a survey course which goes a lot into the hardware and all the techniques with about equal time on PB, APCI and ESI. There is a 5 1/2 day course sponsored by the ACS (Mass Spectrometry: Principles and Practice) that I will be teaching with Jack Watson at Mich State U in July. There are two sessions. This is a very intensive couse with a lot on interpretation of EI spectra (important in PB LC/MS) and a laboratory with LC/MS experimentents. We will have a Waters Integrity in the lab and we will be doing PB LC/MS experiments. To attend one of the ACS courses or get more information, call 1-800-227-5558 and follow the prompts to the continuing education department. Waters will be offereing a customer course for Integrity users in October. This could be a good place to pickup some good applications training on PB LC/MS. Mike Balogh (Waters) and Tom Covey (PE/Sicex) gave a course at the Eastern Analytical Conference held in Sommerset, NJ Nov. 17-18, 1996. I don't know if this course will be offered again this year. I have not had any reports about, but I have seen the manual and it looks like it is a good course. I belive this years ASMS course is only going to be on API. Its title has changed to "LC/MS: The Techniques of Electrospray and APCI" from last year's of "LC/MS: The Art and Practice" Neiter Al Yergey nor or Don Chase are any longer involved. In addition to all of the above courses, Jack Watson and I will be teaching several sessions of our two-day and three-day ACS courses on MS interpretation, which have interpretation of EI spectra as the emphasis. O. David Sparkman Consultant-At-Large Phone: 1-510-754-5003 ****************************************************************************** From: "Dave White" Date: 16 Feb 1997 08:40:24 GMT Subject: Re: About VG 7070E GC/MS Organization: SpectraChrom Software } 1) I am going to order gases (argon, xenon, and helium) and regulators for } the instrument. What grade is necessary? High purity or ordinary? Because of the sensitivity and selectivity of the instrument, you will need high purity gases. } 2) Current computer system came with this used VG7070E is DEC PDP11/24 } minicomputer with VGII/250 software? What kind upgrade options are } available today either hardware or software? How much cost if you can give } quote? The 11-24 system is an antique by todays standards. There are currently two options open to you: 1) Upgrade to a VAX or Alpha system with SIOS. This is a complete replacement for the PDP, Scanner and Acquisition Interface - VG are charging around $60K for this - Research Scientific Services, Inc. have one for sale for $24,000 - see our home page at http://netnow.micron.net/~spectrac/rsshome.htm and go to the used instruments section. 2) Upgrade to a PDP-11/73 system. You would keep your existing Scanner and Interface, and just replace the PDP with a much faster and more reliable one. You can contact Research Scientific Services, Inc. (who are headquartered in Raleigh, NC) via e-mail at spectrac@micron.net, or by telephone at (800) 676-1991. In addition, we are currently developing our own PC based system which will replace the PDP with a PC, and a control box using Digital Signal Processors for scanning and peak processing. This system, with all software, will cost in the region of $22,000. We are planning to have this system in beta by the time ASMS comes around. Dave White Systems Development Research Scientific Services, Inc. ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 16 Feb 1997 20:57:26 GMT Subject: Re: peptide mass calculations Organization: AOL http://www.aol.com I think Fenseleau wrote a good one in Analytical Chemistry within the last ten years. Dick Smith's group might have done one as well. This question is best answered there but,... I think it depends upon the mass of the pieces you are looking for. matt sweeney ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 16 Feb 1997 21:09:22 GMT Subject: Re: Reference papers on FC-43 (perfluorotributylamine) Organization: AOL http://www.aol.com You could calc them yourself using the TSQ view for the purpose or look in The Methods in Enzymology volume "Mass Spectrometry" the rear of the book has many standards in tables. This is a great but a little dated text. They should do another. Matt Sweeney ****************************************************************************** From: Stuart Thomson Date: Mon, 17 Feb 1997 13:02:18 +1100 Subject: Re: peptide mass calculations Organization: Monash University Teunis vanRee wrote: } } Brett Phinney (brettsp@mail.utexas.edu) wrote: } : I am doing a peptide map of a protein I am interested in. For some } : preliminary data I am first doing a trypsin digest and then I am using a } : mass spec. to get the mass of my fragments, with the hope that I will be } : able to determine disulfide bond locations. My question is that when I } : calculate the expected mass of my fragments I can get differing results } : if I calculate the mass using the average mass or the monoisotropic } : mass. Does anyone have any suggestions as to which calculation method I } : should use? Does anyone have a good reference on this subject? } } There's no way you can use the average mass no. The mass spectrometer sees } only monoisotopic ions. So calculate the monoisotopic mass nos. } } Regards I Beg to differ. This question really is, does the resolution of my mass analyser allow the differentiation of the full isotopic distribution at the mass in question? If not then use average masses, as the mass from the mass spec will be such. If so the use monoisotopic masses as the mass from the mass spec will be so. So it's horses for courses Brett, if you can see the isotopic distribution of your molecular ion for your tryptic peptides, then use those masses, if not don't as you'll be out by a couple of mass units. Cheers Stuart Thomson. ****************************************************************************** From: Kurt Benkestock Date: Mon, 17 Feb 1997 10:46:38 +0100 Subject: Re: peptide mass calculations Organization: Pharmacia & Upjohn Brett Phinney wrote: } } I am doing a peptide map of a protein I am interested in. For some } preliminary data I am first doing a trypsin digest and then I am using a } mass spec. to get the mass of my fragments, with the hope that I will be } able to determine disulfide bond locations. My question is that when I } calculate the expected mass of my fragments I can get differing results } if I calculate the mass using the average mass or the monoisotropic } mass. Does anyone have any suggestions as to which calculation method I } should use? Does anyone have a good reference on this subject? } } Thanks a lot } } Brett Phinney It depends on which resolution you have on the mass spectrometer during the analysis. On a quadrupole instrument where the resolution could be maximized to 1000 you have to use both the mono- and the average molecular mass for the calculation. For fragments upp to around 1200-1400 Da monoisotopic mass can be used. Above this value the resolution is not sufficient to resolve the monoisotopic peaks. As a result, you get one broad peak and the average mol mass must be used. Good Luck Kurt Benkestock ****************************************************************************** From: "Robbin Nichol" Date: 17 Feb 1997 13:43:09 GMT Subject: Free Demonstration Training Software for Analytical Techniques. Organization: Cognitive Solutions A free downloadable demonstration package of our SoftBook/ITP packages is available from our Website:- http://www.globalnet.co.uk/~cogsol The topics Available are GC, GC Method development, ICP, AAS, HPLC and MS, the packages are available in American and British versions some are now available in German with all being translated within the next few months. Also available is a MSPowerpoint presentation of our SoftCOSHH COSHH Assessment and Management program. This is supplied with the free MSPowerPoint Viewer. Details of prices etc can be obtained from the downloadable info pack or from me at cogsol@mail.globalnet.co.uk Robbin Nichol B.Sc. M.Sc. Ph.D. MRSC C.Chem. Cognitive Solutions Glasgow UK ****************************************************************************** From: David Vollmer Date: Mon, 17 Feb 1997 10:43:21 -0700 Subject: Finnigan XSQ7000 source heater Organization: * To Finnigan XSQ7000 CI/EI users, We have recently purchased a Finnigan TSQ7000 that comes equipped with the" new-source" design. This new design, however, has given us problems concerning the attachment of the heating element to the source block. The elements seem to break very easily, either by poor design, or maybe we are putting them together incorrectly. Has anyone has the same troubles or does anyone have any insights on how to remedy this situation. Thanks, Dave Vollmer ****************************************************************************** From: PAREESDM@apci.com Date: Mon, 17 Feb 1997 17:26:29 -0500 Subject: Re: peptide mass calculations Organization: * Brett Phinney submitted the following query: }From: Brett Phinney }Date: Fri, 14 Feb 1997 13:40:45 -0800 }Subject: peptide mass calculations }Organization: University of Texas at Austin }I am doing a peptide map of a protein I am interested in. For some }preliminary data I am first doing a trypsin digest and then I am using a }mass spec. to get the mass of my fragments, with the hope that I will be }able to determine disulfide bond locations. My question is that when I }calculate the expected mass of my fragments I can get differing results }if I calculate the mass using the average mass or the monoisotropic }mass. Does anyone have any suggestions as to which calculation method I }should use? Does anyone have a good reference on this subject? }Thanks a lot }Brett Phinney If I understand the question correctly, the answer is dependent on the resolution of the mass spectrometer that you are using. If the instrument has sufficient resolution to resolve the isotopic clusters of the molecular ions, then the masses you will obtain are the isotopic masses and those are the calculated ones to use for comparison. If your instrument has insufficient resolution (e. g., an old-style, non-delayed extraction, non-reflecting time-of-flight used for matrix-assisted laser desorption/ionization of species with masses in excess of a few hundred daltons), then the molecular ion clusters may be non-resolved and the masses measured would be the average masses; a simple combination of all of the isotopes of all of the elements present, taking into account their relative abundances. In this case, the data should be compared to isotopic average molecular weights (plus any cationization that your technique may incorporate). Dave Parees My employer accepts no responsibility for my internet activity ****************************************************************************** From: PAREESDM@apci.com Date: Mon, 17 Feb 1997 17:27:57 -0500 Subject: Re: GC/MS determination of thiophenes? Organization: * The following question was posed: }From: pvang@sci.kun.nl (Peter van Galen) }Date: 14 Feb 1997 08:18:57 +0100 }Subject: GC/MS determination of thiophenes? }Organization: University of Nijmegen, The Netherlands }Summary: How too determine the identity of low molecular weight sulfur }containin }Hello out there, }I was asked wether it is possible too determine the compostion of a gas }mixture containing compounds as CH3SH, C2H5SH, COS, CS2, CH3S-SCH3 and }possibly related compounds. I've given it a shot at a Saturn II equipped }with a HP-1 column, knowing that there was hardly any chance for succes, }but just too see if some evidence for the contents of sulfur could be }found. The only thing achieved was some circumstantial evidence for CH3S }fragmentation. I would like too know what the best column for this }purpose is. And in addition as it is likely to be a one shot experiment, }who, within reasonable distance, could do such an experiment for us. }Thanks in advance, }|___________________________ Peter M. van Galen }|____ Mass Spectrometry, Organic Chemistry Dept. }|__________________ Nijmegen University }|_ Toernooiveld 1, 6525 ED Nijmegen, }Netherlands }|___ FAX 024-3652929, E-mail pvang@sci.kun.nl A column like the HP-1 should be able to separate these species. However, you do not state the concentrations of the species expected or what the matrix gas is. Is it one of these? If so, there might be a challenge separating the other compounds that elute near it. Also, you did not provide any details about your column or temperature program. You may need a longer or thicker film column than the one you are using. I checked two chromatographic column suppliers. Restek shows a separation of these species on an Rtx-1 column (very much like yours), but it is a widebore (0.53 mm) column, 60 m long and with a very thick film (7 um). Yours was probably not like this. This supplier and Supelco also offer capillary columns specifically formulated to separate light sulfur gases, called Rt-Sulfur by Restek and SPB-1 Sulfur by Supelco. In addition, Supelco shows chromatograms of these species on a number of packed columns, but I doubt your instrument is equipped with a jet separator, so that would not be feasible for you. I think it is possible that Poraplot Q, available form J&W (GS-Q) or Chrompack, could also do this separation. Chrompack, based in the Netherlands, might be your best bet for calling to get some advice. Something else to keep in mind is the condition of the injection port. I assume you are analyzing standards of the species of interest and you know the detection limit for these species on your instrument. Do you know that the levels in the sample are above your detection limit? Since you're using a Saturn instrument, which is an ion trap, selected ion monitoring will not decrease your detection limits very much (there is some advantage, in my experience, presumably due to signal averaging since there is no dwell time advantage). As for advising you about a lab, I can't offer any help because I'm not familiar with those in Europe. There should be listings of labs available that do GC-MS, and you can call some and ask some questions until you find one that can do this. Dave Parees My company has no responsibility for my E-mail ****************************************************************************** From: Rick Russo Subject: Re: laser probe on PQII Date: Mon, 17 Feb 1997 18:03:42 -0800 (PST) Organization: * Cathy I don't know much about the particular laser or system you have, but my group does a lot of work with laser ablation. Destroying the cell window means that you have exceeded the approximate 10J/cm2 damage threshold for quartz (assuming the window is quartz which is better be). Sounds like you are too close to the focus of the lens. What is a 'low magnitude lens'? The distance between the lens and cell window is critical, should be close so that the bean diameter at the window is large; not focused. Also, the distance from the sample surface to the window is important. Another thing to look for is reflections from the sample surface back to the cell window, and the lens. Focused reflections can damage optics at the fluences achieved with a nanosecond YAG laser. If 750V lamp voltage is destroying the cell window, might try running the laser at slightly lower power. However, you should check the laser specifications because pulse to pulse stability may be worse at low voltage. We make all of our cells in house, and not aware of any commercial units. Rick >We are trying to install a laser probe on our PQ II . > >The z stage is at the maximum and if we put the flash energy to 750 >Voltage (like described in the manuel) we distroy our laser cell. >We work with the low magnitude lens and the biggest laser cell . >Can anyone help us out. > >P.S. we also are looking for the reference number to buy a new big laser >cell. We already asked Thermogroup Holland but they can't help us. > >Cathy >********************************** ****************************************************************************** From: James Pavlovich Date: Tue, 18 Feb 1997 09:27:28 -0800 Subject: Re: peptide mass calculations Organization: University of California, Santa Barbara Brett Phinney wrote: } } I am doing a peptide map of a protein I am interested in. For some } preliminary data I am first doing a trypsin digest and then I am using a } mass spec. to get the mass of my fragments, with the hope that I will be } able to determine disulfide bond locations. My question is that when I } calculate the expected mass of my fragments I can get differing results } if I calculate the mass using the average mass or the monoisotropic } mass. Does anyone have any suggestions as to which calculation method I } should use? Does anyone have a good reference on this subject? } It depends on a couple of factors which is the best value to use. In your case, tryptic fragments are relatively small so using the monoisotopic mass would be most apprpriate. Generally speaking you should use the monoisotopic mass so long as your instrument has sufficient resolution to distinguish the individual isotopic masses (alway 1 amu apart). However, at mass above ~1500 amu the monoisotopic mass become decreasingly abundant and will eventually be insignificant at masses on the order of several thousand amu. At that point, average mass is more meaningfull even if you have the resolution. Also note that for multiply-charged species produced by electrospray ionization, the isotopic peaks will be closer together by 1/z, and require correspondingly higher resolution to distinguish the isotope peaks. Therefore, use of average mass may be more meaningful when calculating mass from highly charged species, unless you have a very high res instrument. Good luck, James Pavlovich -- ____________________________________________________________________ ____________________________________________________________________ James G. Pavlovich, Ph.D. Mass Spectrometry Facility Phone: 805-893-4252 Chemistry Department FAX: 805-893-4120 University of California Email: pavlovic@chem.ucsb.edu Santa Barbara, CA 93106 ____________________________________________________________________ ____________________________________________________________________ ****************************************************************************** From: "Greg R. Shaw" Date: Tue, 18 Feb 1997 10:52:02 -0800 Subject: Finnigan 4500 for Sale Organization: West Coast Analytical Service For sale: Finnigan 4500 quadrupole GC/MS/DS. 2-1024 mass range, 9610 GC, dual diffusion pump, solids probe, -3kV dynode, Incos Rev 6.5 software (including 42000 entry NIST library), Printronix MVP printer, 70 Mb Winchester drive, Cipher streamer tape. Includes user and service manuals, full schematics. Please Contact: eric.lindsay@wcaslab.com -- Eric Lindsay West Coast Analytical Service eric.lindsay@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com -- Greg R. Shaw West Coast Analytical Service greg.shaw@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: "Greg R. Shaw" Date: Tue, 18 Feb 1997 10:54:47 -0800 Subject: GC/MS Position Available Organization: West Coast Analytical Service Job Title: GCMS Operator Job Description: West Coast Analytical Service, Inc. (WCAS) is looking for chemists that are experienced GCMS operators. This experience must include work on environmental samples for volatiles and semivolatiles as well as screens of unknown materials. Experience with pharmaceuticals and air samples would be an advantage. Must have a minimum of a B.S. degree, over 5 years experience, able to solve problems, repair instruments, only interested in high quality work, can work under strict deadlines, and has excellent organizational skills. Please Contact Eric Lindsay eric.lindsay@wcaslab.com 9840 Alburtis Ave Santa Fe Springs, CA 90670 Voice (562)948-2225 Fax (562)948-5850 -- Greg R. Shaw West Coast Analytical Service greg.shaw@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: sumner@chemvx.chem.tamu.edu (Sumner Lloyd) Date: Tue, 18 Feb 1997 15:03:20 -0600 Subject: Average/Monoisotopic Organization: * Brett Phinney (brettsp@mail.utexas.edu) wrote: : I am doing a peptide map of a protein I am interested in. For some : preliminary data I am first doing a trypsin digest and then I am using a : mass spec. to get the mass of my fragments, with the hope that I will be : able to determine disulfide bond locations. My question is that when I : calculate the expected mass of my fragments I can get differing results : if I calculate the mass using the average mass or the monoisotropic : mass. Does anyone have any suggestions as to which calculation method I : should use? Does anyone have a good reference on this subject? Brett, The mass that you should use is dependent upon the resolving power of the mass spectrometer that you are using. If you have sufficient resolution to separate the isotopes of the ion peak in question than you should use the monoisotopic masses. On the other hand, if the resolving power of the MS is low such as MALDI linear time-of-flight, than you should use the average mass because what you observe in the mass spectrum is the unresolved isotopic profile that is centroided at the average mass. Another suggestion for your interest in disulfide bonds is the alkylation of disulfides with methyl or ethyl iodide for the primary determination of the number of disulfide bonds. The number of disulfides is determined by shifts in the mass of the suspect protein as determined by MALDI or ESI. Further, higher order structures can be investigated by selective alkylation of disulfide bonds and their effect on structure. Best Regards, Lloyd W. Sumner, Ph.D. Associate Director, The Laboratory for Biological Mass Spectrometry Department of Chemistry Texas A&M University College Station, TX 77843-3255 Voice #(409)845-8404 Facsimile#(409)845-4719 Email Sumner@chemvx.tamu.edu ****************************************************************************** From: sumner@chemvx.chem.tamu.edu (Sumner Lloyd) Date: Tue, 18 Feb 1997 15:05:18 -0600 Subject: VG Upgrades/Gases Organization: * }HK WANG, Ph.D. Wrote }Research Associate Professor }Room 302, Beard Hall }Univ. of North Carolina at Chapel Hill, }Chapel Hill, NC 27599-7360 }Tel: 919-966-5908 }FAX: 919-966-3893 }Email: hwang@gibbs.oit.unc.edu }Dear GC/MS experts: } }I am new to this newgroup. Please foregive me if my questions are not new }to you. } }I will in charge of VG7070E Mass spectrometer for my department. Two }Questions here: } }1) I am going to order gases (argon, xenon, and helium) and regulators for }the instrument. What grade is necessary? High purity or ordinary? } }2) Current computer system came with this used VG7070E is DEC PDP11/24 }minicomputer with VGII/250 software? What kind upgrade options are }available today either hardware or software? How much cost if you can give }quote? } }Thanks for your attention. Dr. Wang, I recomend, the highest purity of gases for your system (99.995%). Although 99.99%+ is the highest purity sold, these gases are seldom this pure. It has been my experience that the lower the gas grade, the more it deviates from its quoted purity level. (quite skeptical) Thus, it is best to go with the highest purity available. I recomend xenon over argon as a FAB gas because it provides better sensitivity. Although the initial expense is quite high, I have been operating from the same 100L bottle for ~3 years with a fairly heavy load (200 to 400 samples/month) but, be careful of leaks!! (VG-70S). I also believe you should utilize high purity gases for GC/MS and CID MS-MS to minimize background in GC (water, oxygen, nitrogen, argon,etc.) and any unusuall ion/molecule reactions in CID. There are two computers systems above the PDP 11/24. They are the PDP 11/73 and the Opus/Sios systems. The Opus/Sios is both a hardward and software upgrade and will cost you approximately $68K, but you should seek a more accurate quote from VG. They have a web page at http://www.Micromass.co.uk. and would be more than happy to get you a quote. Best of Luck, Lloyd W. Sumner, Ph.D. Associate Director, The Laboratory for Biological Mass Spectrometry Department of Chemistry Texas A&M University College Station, TX 77843-3255 Voice #(409)845-8404 Facsimile#(409)845-4719 Email Sumner@chemvx.tamu.edu ****************************************************************************** From: SKnutson Date: Tue, 18 Feb 1997 15:49:05 -0800 Subject: Help: Chemstation Automation Organization: * Hello; I am new to this newsgroup, please don't flame me if this topic has already been covered, point me in the right direction. I have a HP5972 MSD using G1034C (rev. C.03.02) Chemstation for acquisition. This instrument is used primarily for an in-house pest. screen. The analysis is routine and very repetitive. I am interested in writing a command macro to control the "Sample Name" field from an external data file (pref. CSV text file). As our product is sampled in the production area the techs. scan a variety of information off of the container using a bar code reader. This data file contains the "Sample ID" which i would like to automate into the Sequence file. The Als position, method name, data file name can all be the same from day to day the only thing that changes is the Sample ID. I have opened the sequence.s file which HP stores in it's chemstaion\sequence directory using a text editor and it appears to be of the encoded binary type, it's not a straight text file. Has anyone done anything similar to this or have any information of how the sequence.s files are encoded? Any help would be appreciated. thanks in advance scott sknutson@localaccess.com ****************************************************************************** From: y2-sano@eisai.co.jp (Yoshihisa Sano) Date: Wed, 19 Feb 1997 19:41:53 +0900 Subject: XSQ7000 heated capillary Organization: * To Finnigan XSQ7000 ESI/APCI users, We have used TSQ7000 equipped with a semi-micro HPLC system for quantitative analysis of drugs and its metabolites in biological fluids. We recently found the heated capillary which metallic surface was peeled off from the body during analysis, and broken pieces dispersed inside the housing. Has anyone experienced such case and does anyone know the causes of it? Aqueous methanol (or acetonitrile) containing 0.1% TFA is usually employed as a mobile phase at a flow rate of 0.1-0.2 ml/min for more than 24 hours continuously. Is the solvent too acidic to be used as a mobile phase for such long period? Thanks for any help, Yoshihisa Sano Drug Metabolism Research Section, Eisai Co., Ltd., Tsukuba, Ibaraki, 300-26 Japan (Feb. 19, 1997) Yoshihisa Sano ****************************************************************************** From: Robert Locht Date: Wed, 19 Feb 1997 13:20:44 +0100 Subject: data acquisition software Organization: Universite de Liege I am looking for a data acquisition and analysis software. Actually I use the Asystant Plus 1.10 package for collecting and processing mass spectra. It’s analysis tools are quite good but it's not handly (it runs in DOS and supports no mouse). Since it seems that there are not more evoluated versions of Asystant+ I would like to find or another package . It's important for me to have the possibility to smooth and fit whith a user defined function data vectors of at least 2000 points. A timer programmer would be interesting, too. I have tried TestPoint , DasyLab and WorkbenchPC but none of them offers such possibilities. Could anyone help me please? Thank's in advance. Antuan Hoxha Département de Chimie Générale B6 Université de Liège 4000 Sart Tilman tel: 0032.4.3663429 Fax:0032.4.3663413 e-mai: s943462@student.ulg.ac.be ****************************************************************************** From: Paul_Peterman@nbs.gov (Paul Peterman) Date: Thu, 20 Feb 1997 17:05:07 -0700 Subject: RE: WANGCO Disk Drive Organization: * In response to Dr. Huu van Tra who wrote: }From: Tra@uunet.uu.net, Huu@uqam.ca (Dr. Huu van Tra) }Date: 12 Feb 1997 21:31:05 GMT }Subject: WANGCO Disk Drive }Organization: UQAM }I'm looking for one or two used (but in working conditions) } WANGCO Disk Drive(s) for a NOVA-3 Data System. }This data system is used for collecting data and control the FINNIGAN }Mass Spectrometer Model 4000. }If anyone wants to dispose them, please contact: } }Prof. Huu van Tra }Dept. of Chemistry }Universite du Quebec a Montreal }Montreal (Qc) Canada } }E-Mail: TRA.HUU@UQAM.CA] I suggest you contact: LGC (Los Gatos Circuits) 2030 Fortune Drive, Suite A San Jose, CA 95131 (408)-946-0969 or (800)-999-0599 Good luck, Paul Peterman, Midwest Science Center, USGS, Columbia, MO ****************************************************************************** From: walther@servier.fr (Ph. VAYER) Date: 21 Feb 1997 12:27:54 GMT Subject: Trap vs Quad in structural analysis Organization: servier.fr Does anybody have an idea of the performances of ion traps vs quadrupoles for the structural characterisation of small molecules (MW 1500)? For non peptide molecules did MS(n) from ion traps give more structural informations than classical tandem quadrupoles? Thanks in advance, Ph. VAYER ****************************************************************************** From: Spiros Pergantis Date: Fri, 21 Feb 1997 14:05:16 +0100 Subject: Low-energy CID of M+H vs. M+Na Organization: * We are currently working on the electrospray analysis of small peptides, which under our operating conditions give us very intense [M+Na] and low [M+H] ions. Unfortunately our peptides are N and C-terminally blocked and have no obvious sites for protonation. The carrier solution we are using consists of 50%ACN/0.1-1% HCOOH. When we change the instrument conditions we can achieve approximately equal intensity for the [M+Na] and [M+H] ions, but the overall sensitivity is very much reduced. Because we are trying to optimise a protocol for sensitivity we would like to stick to the conditions that give us the highest sensitivity and hence the [M+Na] ion. This however may be a problem because we eventually want to do low-energy CID on the pseudomolecular ion, which on our system is the [M+Na]. From the literature we have discovered that the [M+H] and [M+Na] ions give different daughter spectra under low energy conditions. However, we are not quite sure if the low-energy CID spectra originating from the [M+Na] ion will be as informative for sequencing the peptides we are interested in. Does anybody have any experience in this matter? Any suggestions would be greatly appreciated. Thanks in anticipation Spiros A. Pergantis ************************************** Spiros A. Pergantis Postdoctoral Fellow Department of Mass Spectrometry Faculty of Chemistry Utrecht University F.A.F.C. Wentgebouw Sorbonnelaan 16 3584 CA Utrecht, The Netherlands Tel.: +31-30-2536794 Fax: +31-30-2518219 ************************************** ****************************************************************************** From: milton@mailgate.navsses.navy.mil (Walter Milton) Date: 21 Feb 1997 16:27:17 GMT Subject: Nitric Oxide Concentration Via GC/MS Organization: "CARDEROCKDIV CDNSWC" Hi, I'm new to GC/MS an I'm looking for methods or information on how to analyse for the following components: NOx; CO2;CO;& O2. Any help would be appericated. I have an HP 5890 Series II GC/MS. Please post & E-Mail to milton@mailgate.navsses.navy.mil Thanks Walt ****************************************************************************** From: Keith Martinko Date: Fri, 21 Feb 1997 11:26:19 -0600 Subject: Re: Help: Chemstation Automation ... Possible solution ? Organization: ProLab Resources, Inc. Scott : Originally, I had sent you a possible solution to your HP MS ChemStation automation problem directly to your e-mail address. However, I have noticed that other subscribers to this newsgroup have experienced similar problems with the ChemStation software ... so I thought I would "broadcast" this response for everyone's benefit. My company, ProLab Resources (http://www.prolab.com), specializes in developing software to enhance the productivity and performance of the HP GC and MS DOS ChemStation software. This service developed in response to a large number of HP customers that contacted us about "extending" the feature set of the ChemStation, independently of HP. Over the course of working with various customers on LIMS integration, custom reporting and other features, we developed a set of products called EXTEND, that add about 25-30 new features onto the HP GC and MS ChemStation platforms. As we receive new suggestions from our customer base, we continue to add new features. As far as your problem is concerned, we have also run into "snags" concerning access to the the structure of the main sequence file under the .S subdirectory. Your problem, however, is similar in nature to one we solved recently for a large environmental laboratory. In this case, they wanted sample information from their LIMS (hold date, due date, prep batch, ...) to be automatically extracted from the LIMS and stored with each data file. Once stored, they could use this information to modify the ChemStation report headers to their liking. On the back end, we were able to automatically send the results files (quant and/or libsearch) directly back to the LIMS. We ran into the same problem on the front end trying to use the HP sequencer to pull out information from the LIMS and store it with each data file as it was being acquired. However, with more careful thought ... a solution appeared that you maybe able to utilize. Instead of extracting this information at data collection, why not extract and save this information at the instance when the data file is loaded. Every data file must be loaded before any processing can be done (either manually through the ChemStation processing system or automatically during batch processing). Essentially, we had the customer construct a variable of the format SAMPLEID#-CSVFILE and place this information in the MISC INFO field for each data file in the sequence. Upon data load, the software would look in the CSVFILE specified for information on SAMPLEID# and either update the default sample information (SAMPLE NAME, MULTIPLIER, ...) or add in new information for the data file (through use of an .INI file stored under the .D subdirectory). After that, all of the information was available to customize reports, perform new calculations (based on sample prep info) and so forth. On the back end, we were able to use some of the information extracted from the LIMS on the front end to automate the sending of results automatically on the back end. Obviously, there are a few more details than this that you will need to work out ... however, if you need any more help please contact me. Hope this has been helpful !? Best regards, Keith Martinko President ProLab Resources, Inc. prolab@ix.netcom.com http://www.prolab.com ****************************************************************************** From: candymkr@aol.com (Candymkr) Subject: D 5769 Aromatics in gasoline by GC/MSD Date: 21 Feb 1997 20:40:41 GMT Organization: AOL http://www.aol.com Hello! I am interested in setting method D5769 in my lab. I look for information in software and hardware needed. Thanks RReyes ****************************************************************************** From: "J. David Malinsky" Date: Fri, 21 Feb 1997 16:37:06 -0700 Subject: Re: WANGCO Disk Drive Organization: The University of Calgary } }From: Tra@uunet.uu.net, Huu@uqam.ca (Dr. Huu van Tra) } }Date: 12 Feb 1997 21:31:05 GMT } }Subject: WANGCO Disk Drive } }Organization: UQAM } } }I'm looking for one or two used (but in working conditions) } } WANGCO Disk Drive(s) for a NOVA-3 Data System. } }This data system is used for collecting data and control the FINNIGAN } }Mass Spectrometer Model 4000. } }If anyone wants to dispose them, please contact: } } } }Prof. Huu van Tra } }Dept. of Chemistry } }Universite du Quebec a Montreal } }Montreal (Qc) Canada } } } }E-Mail: TRA.HUU@UQAM.CA] Another good source would be: Scott McIntyre & Associates, Inc. P. O. Box 82115 Rochester, MI 48308-2115 Phone: (810) 299-0040 FAX: (810) 299-0041 E-mail: sma4dg@aol.com They have a large selection of Data General components. Dave -- J. David Malinsky The University of Caglary Department of Chemistry malinsky@chem.ucalgary.ca ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 23 Feb 1997 21:29:28 GMT Subject: Re: Low-energy CID of M+H vs. M+Na Organization: AOL http://www.aol.com I would try to reduce sodiation first of all. This could be done be using rp-hplc connected straight to the esi source. Short coloumns work fine i use plrp packing in many cases. Some companies such as microm-bioresources in auburn CA USA sell small "trap cartridges" I have used these under these conditions to great success. You load your sample in 10x dilution in a highly aqueous solution onto the prequilibrated material which is held in a small holder in the injector where the sample loop would be. This is followed by an aq wash which removes the salts. The loop is then switched and the packing material then releases the peptide due to the higher (60-70%) organic level which is being continously pumped to the ESI-source and now includes the sample trap cartridge in its flow path. This allows one to use either an hplc at higher flow rates to quickly set up an autosampler method which when combined with two flow sorces allows this to be completely automated. It is possible to do this without an hplc using syringe pumps or even better a fluid delivery module (sold also by microm) which basically uses the bomb method of pressurizing a container of lc eluent with compressed gas. One might use a low-flow syringe pump to enable a slow enough elution to allow the operator to optimize and acquire data. The other issue is M+H vs M+Na and while M+Na may in some cases be useful quantitativly the great body of qual. work on ESI protein sequencing utilizes M+H. And with good reason. The Na is very tightly bound and if it is the charge carrier may leave at m/z 23 Na+ which yields nothing. I have also found the increased scattering that is occuring in the collision region due to the much higher energies usually required for progeny production from sodiated species results in a rolloff insensitivity with the requisite increasing energies. Often times what you get when you "tune" one of these instruments to get more equal protonated and sodiated species is a detuning as you describe. This is often times what I interpret to be the production of source CID products from the M+Na species some of which form into the M+H possibly and others which dissappear as the Na+ charge carrier is lost. So what you may be doing is increasing source harshness and so producing reduced populations of sodiated species through collisions (appearing as a "reduction" of sodiation) Check to see if the tune conditions increase ion energies and watch as you seem to be absolute counts. I'd just zero in on that m+H and tune to optimize the source on it's absloute intensity (completely ignoring them+Na) then do an HPLC type short col. and you should be in buisness. You may never get rid of all the Na. It is a chore to look at the whole process stream to locate and eliminate possible sources. Few analytical inst. in the biochem lab can detect Na as well as the ESI system so your efforts (and paranoias) may seem unusual to others but that is because you can see it with the inst. Look at solvents, containers, pumps, tubing and reagents as well as sample work up. Runing many Na contaminated samples with out clean-up will lead to invariable sodiation in subsequent runs. Dissolving up any sample in a glass vial prior to running it is asking for this problem. I usually scoop out in to an plastic eppie any solid material provided to me in glass and then add the dissolution solvent in the plastic eppie (avioding exposure of my sodium chelator,... I mean sample to large expanses of natriated silica (the vial) while in solution. There is a nice method for glycoform analysis that uses reduced and methylated species run in Na+ (about 5-15 mM I think) which to an educated eye can aid carbohydrate analysis using ESI. I have seen so few peptides which just won't take a charge that I tend to think it is not a fundemental characteristic of the species at hand. Good luck. matt sweeney ****************************************************************************** From: sjm@kore.co.uk (Steve Mullock) Date: Mon, 24 Feb 1997 11:49:35 GMT Subject: Re: data acquisition software Organization: Kore Technology Ltd. Robert Locht wrote: }I am looking for a data acquisition and analysis software. }... You might consider GRAMS 32 from Galactic Inc. We use it with our mass spectrometer products, though it is actually designed to cope with a wide range of spectral style data. There is a wide range of processing options, for details see, for example: http://www.kore.co.uk/users/ab48/gramsint.htm regards Steve Mullock ****************************************************************************** From: bardonia@aol.com (Bardonia) Date: 24 Feb 1997 15:14:39 GMT Subject: Commercial Mass-spec Labs Organization: AOL http://www.aol.com Does anybody know of a laboratory that will perform direct probe mass spectrometric analyses such as FAB and/or DCI? If so, what do they charge and what is included in the price? Cordially Mike ****************************************************************************** From: candymkr@aol.com (Candymkr) Date: 24 Feb 1997 15:52:14 GMT Subject: HP-5970 and Perfluorotributylamine abundances. Organization: AOL http://www.aol.com I got a refurbished 5970 and each day I have used std autotune. Abundances for ions changed even >100%. Started to do a user tune. Still the same. Source, quadrupole, filaments, all electronic boards, etc. changed... leaks, columns ports etc. are good. Two HP approved companies have been here and changed everything included the whole instrument. Still the same Any advise? ****************************************************************************** From: "Greg R. Shaw" Date: Mon, 24 Feb 1997 10:21:31 -0800 Subject: Finnigan 4500 For Sale Organization: West Coast Analytical Service For sale: Finnigan 4500 quadrupole GC/MS/DS. 2-1024 mass range, 9610 GC, dual diffusion pump, solids probe, -3kV dynode, Incos Rev 6.5 software (including 42000 entry NIST library), Printronix MVP printer, 70 Mb Winchester drive, Cipher streamer tape. Includes user and service manuals, full schematics. Please Contact: eric.lindsay@wcaslab.com -- Eric Lindsay West Coast Analytical Service eric.lindsay@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: "Greg R. Shaw" Date: Mon, 24 Feb 1997 10:23:12 -0800 Subject: GC/MS Position Available Organization: West Coast Analytical Service Job Title: GCMS Operator Job Description: West Coast Analytical Service, Inc. (WCAS) is looking for chemists that are experienced GCMS operators. This experience must include work on environmental samples for volatiles and semivolatiles as well as screens of unknown materials. Experience with pharmaceuticals and air samples would be an advantage. Must have a minimum of a B.S. degree, over 5 years experience, able to solve problems, repair instruments, only interested in high quality work, can work under strict deadlines, and has excellent organizational skills. Please Contact Eric Lindsay eric.lindsay@wcaslab.com 9840 Alburtis Ave Santa Fe Springs, CA 90670 Voice (562)948-2225 Fax (562)948-5850 -- Eric W. Lindsay West Coast Analytical Service eric.lindsay@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: Chris Elhardt Date: 24 Feb 1997 19:59:53 GMT Subject: Re: Help: Chemstation Automation Organization: Texas Department of Health Have you contacted HP yet? Although we don't use barcode software, there appears to be some built into G1034C. chris Elhardt **not-officially-speaking-for** Texas Department of Health SKnutson wrote: }Hello; } }I have a HP5972 MSD using G1034C (rev. C.03.02) Chemstation for }acquisition. This instrument is used primarily for an in-house pest. }screen. The analysis is routine and very repetitive. I am interested in }writing a command macro to control the "Sample Name" field from an }external data file (pref. CSV text file). } }As our product is sampled in the production area the techs. scan a variety }of information off of the container using a bar code reader. This data }file contains the "Sample ID" which i would like to automate into the }Sequence file. The Als position, method name, data file name can all be }the same from day to day the only thing that changes is the Sample ID. } }I have opened the sequence.s file which HP stores in it's }chemstaion\sequence directory using a text editor and it appears to be of }the encoded binary type, it's not a straight text file. } }Has anyone done anything similar to this or have any information of how }the sequence.s files are encoded? Any help would be appreciated. } }thanks in advance }scott }sknutson@localaccess.com } } ****************************************************************************** From: "John Bartmess" Date: Mon, 24 Feb 1997 16:30:24 EST Subject: Mailing List for STM-S Organization: Univ. of Tenn. Dept. of Chemistry This is information for readers of sci.techniques.mass-spec (STM-S) to pass along to those who cannot read Netnews, for whatever reason, but do have access to e-mail. There is a mailing list that will e-mail the contents of STM-S to anyone who wishes to subscribe. This mailing list is maintained at the Chemistry Dept., University of Tennessee, Knoxville by John Bartmess, . This list is "public," which means that anyone may subscribe. It is also a one-way system, in that anything that subscribers want posted to STM-S should be sent to the moderator of that group, at . Other than a subscribe/unsubscribe request, anything sent to this mailing list address, , will go into the bit bucket. No human will see it. Likewise, any requests to subscribe/unsubscribe sent directly to me, , will not be acted on. I welcome personal e-mail *about* mass spec topics. {Ion Thermochemistry To Go!} The contents of the newsgroup will be mailed to subscribers as an ascii file twice a week, typically late afternoon Mondays and Thursdays. Subscribers thus will not get each post on the group separately, but many of them (typically 4-15) concatenated into one file. TO SUBSCRIBE TO THIS LISTSERVER: Send a message to: with 1. *no subject*, and 2. the *only* text being: SUB STMSLIST TO UNSUBSCRIBE FROM THIS LISTSERVER: Send a message to: , with no subject, and the only text being: UNSUB STMSLIST Comments regarding STM-S should be sent to the Moderator of that group, David Bostwick if you wish to keep them private, or to the Newsgroup itself , if you wish to make them public. REMINDER on e-mail addresses: for article submission. for private questions concerning the group and its operation. to subscribe or unsubscribe from the Mailing List. ONLY if you are having problems with getting the Mailing List via the maiser address above. John --=--=--=--=--=--=--=--=--=--=--=--=--=--=--=--=--=-=--=--=--=--=--=--=- | John Bartmess | Organic Chem || my opinions, being antithetical | | Dept. of Chem., U. of Tennessee || to "big time" football, cannot | | Knoxville TN 37996-1600 || be those of the Univ. of Tennessee| | (423) 974-6578 Fax: 974-3454 || "Anions are more fun!" | | jbartmess@utk.edu http://novell.chem.utk.edu/bartmess.htm | --=--=--=--=--=--=--=--=--=--=--=--=--=--=--=--=-=--=--=--=--=--=--=--=- ****************************************************************************** From: see@body.for.email.address (R. E. Haufler) Date: Tue, 25 Feb 1997 13:45:32 GMT Subject: Re: data acquisition software Organization: R. E. Haufler Galactic enterprises (the company that vends Grams/32) also has their own website: http://www.galactic.com We are also authorized resellers of Galactic's software: http://www.comstockinc.com R. E. Haufler Senior Scientist Comstock Incorporated rehaufler@comstockinc.com ****************************************************************************** From: bernd alexander walter gallasch Date: Tue, 25 Feb 1997 16:27:34 +0000 Subject: Re: HP-5970 and Perfluorotributylamine abundances. Organization: Uni-Bayreuth, Arbeitskreis Spiteller Candymkr wrote: } } I got a refurbished 5970 and } each day I have used std autotune. } Abundances for ions changed } even >100%. } Started to do a user tune. } Still the same. } } Source, quadrupole, filaments, all electronic boards, } etc. changed... leaks, columns ports etc. are good. } } Two HP approved companies have been here } and changed everything included the whole } instrument. } } Still the same } } Any advise? Hi unknown, do you tested leaks with an steel bottle of argon? In the UserTune(EDIT(Aquis)) Section of you can change the masses for repeat ProfScan. If you set the values to 18, 28 and 40 amu you have a very good method to check for leaks. Now you have to blow with the argon on all parts (septum, ferrules ...) Maybe this will help You, sincerely Bernd. ****************************************************************************** From: PAREESDM@apci.com Date: Tue, 25 Feb 1997 10:42:42 -0500 Subject: RE: Commercial Mass-spec Labs Organization: * }bardonia@aol.com (Bardonia) }Does anybody know of a laboratory that will perform direct probe mass }spectrometric analyses such as FAB and/or DCI? If so, what do they charge }and what is included in the price? }Cordially }Mike In response to this query: there are many commercial labs that specialize in mass spectrometry and most do these analyses. Ones you might call to inquire include Oneida labs (315-736-3050) and Shrader labs (313-894-4440). I believe Micromass also still offers a commercial service. In addition, many universities perform analyses for outside organizations. Among others, the University of Nebraska and Washington University (St. Louis) are two that I believe do this. Dave Parees My company is not responsible for my responses ****************************************************************************** From: niea Date: Wed, 02 Jan 1980 15:24:07 -0800 Subject: ASE Organization: National Institute of Environmental Analysis, EPA/Taiwan The Accerlerated Solvent Extraction system (Dionex) gave a synthesyzed oil-like substance when silica gel was used as volumn make up. The temperature is 175 deg C at 2000 psi with Toluene as solvent. Comments are appreciated. Calvin Sheu National Institute of Environmental Analysis EPA Taiwan/ROC ****************************************************************************** From: "Dave White" Date: 26 Feb 1997 17:21:18 GMT Subject: Re: Commercial Mass-spec Labs Organization: SpectraChrom Software } } }bardonia@aol.com (Bardonia) } }Does anybody know of a laboratory that will perform direct probe mass } }spectrometric analyses such as FAB and/or DCI? If so, what do they charge } }and what is included in the price? } }Cordially } }Mike } One lab that I can recommend is Twin City Mass Spectrometry Inc. out in St. Paul Minnesota. This lab can handle FAB (as well as other methods), and can also handle the special 'one off' types of sample that don't fit in with a standard method. They have a very good FAB analyst, Sean Murray, on their team. You can reach them at (612) 407-0965 or check out their web site at http://www1.minn.net/~tcms/ Dave White Research Scientific Services, Inc. ****************************************************************************** From: gs_klinger@pnl.gov (George S Klinger) Date: Wed, 26 Feb 1997 18:05:30 +0000 (GMT) Subject: Re: HP-5970 and Perfluorotributylamine abundances. Organization: Battelle Pacific Northwest National Labs (PN2L) You didn't mention whether or not the level of electronic grass(random background signal) was constant from day to day(with the PFTBA valve closed of course!) If it is, I suspect that the restrictor from the PFTBA supply might be partly blocked. If not, then maybe it's some type of ion source problem. Is the pressure you read on the ion gauge constant each time you opperate the system? HP5970s have a very pressure dependent response, so check your pressure and column flow each time you tune. I usually leave the PFBTA valve open and let the instrument scan for a few minutes before tuning, since the filament is the only thing that heats up the source in that instrument... George In article <5eskhl$i3n@acmey.gatech.edu>, candymkr@aol.com says... } }I got a refurbished 5970 and }each day I have used std autotune. }Abundances for ions changed }even >100%. }Started to do a user tune. }Still the same. } }Source, quadrupole, filaments, all electronic boards, }etc. changed... leaks, columns ports etc. are good. } }Two HP approved companies have been here }and changed everything included the whole }instrument. } }Still the same } }Any advise? } } ****************************************************************************** From: "Tom Cromartie" Date: 27 Feb 1997 00:11:19 GMT Subject: Re: XSQ7000 heated capillary Organization: Zeneca Ag Products Yoshihisa Sano wrote in article <5eeue4$h9u@acmey.gatech.edu>... } To Finnigan XSQ7000 ESI/APCI users, } } We have used TSQ7000 equipped with a semi-micro HPLC system for } quantitative analysis of drugs and its metabolites in biological fluids. } We recently found the heated capillary which metallic surface was peeled } off from the body during analysis..... Yoshihisa, I've used similar solvents for 2 years with no problem. However I rarely use TFA, I usually use HCO2H or HOAc. Still I doubt TFA should have caused your problem. The screws holding the API chamber onto the flange are plated, however (gold?), and the plating can flake off if a non-plastic Philips screwdriver is used. Any chance your flakes were from the screws, not the heated capillary? Bruce These comments are my opinions and in no way represent those of my employer. ****************************************************************************** From: "Mark A. Hanning-Lee" Date: Wed, 26 Feb 1997 16:37:39 -0800 Subject: vuv lamps for PI Organization: Prodigy Internet Could anyone recommend a manufacturer of vuv lamps, or a manufacturer of power supplies for vuv lamps? These would be used for photoionization work. We could use either steady or pulsed lamps. I searched Thomas Register online but there are no entries for "vacuum ultraviolet lamp", and too many entries for "ultraviolet lamp"! We are using lamps and power supplies by Resonance Ltd and by Cathodeon, but neither completely meets our needs. Someone kindly recommended Ushio excimer lamps, but they do not reach wavelengths shorter than 172 nm. Look forward to your responses, Mark Dr. Mark A. Hanning-Lee Syagen Technology 4036 Brighton Circle Cypress, CA 90630 Tel. and FAX (818) 796 5692 E-mail: markhl@prodigy.net ****************************************************************************** From: pricci5@aol.com (PRicci5) Date: 27 Feb 1997 04:41:24 GMT Subject: ms search libraries Organization: AOL http://www.aol.com I would appreciate some suggestions from the group on the best MS libraries to use for forensic drug identification. In our lab we are currently using older 1990 PMW and NIST library databases. These do not include newer drugs and steroids. Any suggestions on what to upgrade to. What is considered the best for this type of analysis? We are using HP mass specs and Chemstation software. Any info would be greatly appreciated. BTW, in my net travels I came across a current, new drug database that is free to download! Use a search engine to find the MASS SPECTROMETRY DATABASE COMMITTEE. You can get all the info on it and download it there. Phil ****************************************************************************** From: bernd alexander walter gallasch Date: Thu, 27 Feb 1997 11:01:50 +0000 Subject: to HP MS-ChemStation 3.x (Pascal) Users Organization: Uni-Bayreuth, Arbeitskreis Spiteller To all Users o the pascal software for the HP MS-ChemStation 3.x I'am using a HP 9000/300 with MS-ChemStation 3.2 and need to transfer files onto DOS or Linux formatted diskettes. Does somebody have a program either for the pascal computer to save chromatograms and spectra on DOS or Linux formatted diskettes or a program for DOS or Linux platforms to read pascal formatted diskettes? Please answer to: bernd.gallasch@uni-bayreuth.de ****************************************************************************** From: Arnd Ingendoh Date: Thu, 27 Feb 1997 11:24:33 +0100 Subject: MS/MS on ion traps or QQQ Organization: * Dear Dr. Vayer, I am responding on your question in the internet: "Does anybody have an idea of the performances of ion traps vs quadrupoles for the structural characterisation of small molecules (MW 1500)? For non peptide molecules did MS(n) from ion traps give more structural informations than classical tandem quadrupoles?" Please lt me introduce my self first. My name is Arnd Ingendoh, I am the application manager for ion traps at Bruker in Bremen, Germany. As you might know, Bruker produces the ESQUIRE ESI ion trap. Regarding fragmentation, in general the following statement can be given: In contrast to triple quadrupole (QQQ) instruments where multiple fragmentations may occur during the passage of the ions through the collision region of Q2, in traps ions are not longer excited after the first fragmentation (because they are not in resonance with the dipolar field frequency applied at the end caps any more). Thus, all product ions are the result of a single-step fragmentation. As a consequence, ion trap MS/MS spectra may show a smaller number of product ions than triple quadrupole spectra in some cases. The disadvantage may be that a higher number of MS/MS steps is needed to reveal the complete structure of an investigated molecule. The advantages of single-step fragmentations, on the other hand, are an easier to interprete and clear series of spectra as well as the possibility to follow fragmentation pathways in molecules more unambiguously. We have been able to show that in various examples. If you are interested, please let me know. I can send you some of the MS/MS spectra we have taken on various compounds then. Best regards, Arnd Ingendoh =================================================== Dr. Arnd Ingendoh Bruker-Franzen Analytik GmbH Fahrenheitstr. 4 28359 Bremen Germany Phone / Fax: +49-421-2205 -244 / -104 email: ai@bfa.de =================================================== ****************************************************************************** From: Chris Elhardt Date: 27 Feb 1997 16:30:14 GMT Subject: Re: HP-5970 and Perfluorotributylamine abundances. Organization: Texas Department of Health I asked our HP service rep while he was working on another of our gc/ms. He's seen this before and has been able to resolve it by replacing lens stack elements, especially the repeller and drawout tube. chris elhardt candymkr@aol.com (Candymkr) wrote: }I got a refurbished 5970 and }each day I have used std autotune. }Abundances for ions changed }even >100%. }Started to do a user tune. }Still the same. } }Source, quadrupole, filaments, all electronic boards, }etc. changed... leaks, columns ports etc. are good. } }Two HP approved companies have been here }and changed everything included the whole }instrument. } }Still the same } }Any advise? } } ****************************************************************************** From: Christopher Kaine Date: Thu, 27 Feb 1997 19:58:52 -0800 Subject: Re: MS/MS on ion traps or QQQ Organization: OneNet Communications HUB News Server Hello; This is a VERY GOOD explanation!!! But we should not forget some very important points of MS/MS on an Ion Trap product. First a few good points, because of the 'storage' time available, one should be able to increase the population of the ions of interest. And the ability to do MS^n(where n>3) experiments. Some points to consider, certain MS/MS experiments are either very difficult or impossible to perform, i.e., Parent Scans, Neutral Loss. I hope this is of some use. Cordially; Chris Kaine ****************************************************************************** From: chrisjcr@aol.com Date: 28 Feb 1997 04:36:03 GMT Subject: Chromatography Conference Organization: AOL http://www.aol.com The Western Carolinas Chromatography Discussion Group invites you to participate in their 12th Annual Chromatography Conference, to be held in Greenville, South Carolina, on April 24, 1997. The conference will feature an exhibition of chromatography related equipment and supplies, as well as numerous technical presentations. An award will be presented for the best student paper. If you wish to present a paper, you may contact: Sherrill King National Starch and Chemical Corp. P.O. Box 578 Woodruff, SC 29388 (864) 969-6719 Sherrill.King@nstarch.com If you wish to participate in the exhibition, contact: Joel Barabas Morton International P.O. Box 3089 Greenville, SC 29602 (864) 292-5718 LJBarabas@aol.com Additional information, as well as numerous chromatography related links can be found at our web site - http://members.aol.com/chromgroup Chris Richardson W.R. Grace, Cryovac Division ChrisJCR@aol.com Chris.Richardson@corp.wrgrace.com http://members.aol.com/chrisjcr ****************************************************************************** From: Ron_Cochran@EMAIL.JRC.COM Date: Fri, 28 Feb 97 10:59:42 EST Subject: HP MSD Datasystem Organization: * We have an HP 5971A MSD for which the data system runs on an HP PC Clone under Windows. Can someone tell me a way to get chromatogram or spectral plots to the standard Windows clipboard, so that they can be dealt with by some other Windows software - like Powerpoint, for example? My goal is to be able to annotate such plots easily. I am not aware that we can currently do such annotation under our HP software. Thank you! R.L. Cochran James River Corp. Ron_Cochran@email.jrc.com ****************************************************************************** From: david.bostwick@chemistry.gatech.edu (David Bostwick) Date: 28 Feb 1997 17:28:49 GMT Subject: ADMIN: Power outage Organization: Georgia Tech The final stage in the UPS installation will take place this weekend, from 0400 Saturday, March 1, until 0700 Tuesday, March 4. E-mail will be accepted, but the news server will not be available, so new articles cannot be approved until Tuesday. We are sorry for the inconvenience, and will begin posting articles again as soon as possible. ****************************************************************************** From: genesis@magicnet.net (Val Taylor) Date: Fri, 28 Feb 1997 19:13:30 GMT Subject: WWW.TECNET.COM - GLOBAL USED EQUIPMENT DATABASE Organization: Genesis Scientific/Tecnet Your one stop hi-tech used equipment source is at WWW.TECNET.COM. We specialize in electrical, semiconductor, optical, and mechanical engineering (production, test, and laboratory) equipment. Whether your expanding your lab, replacing a piece of equipment, or opening a new research/production based business, we got all from office furniture and equipment to lasers and wafer handlers. We will also list your surplus assetts on our database for free! Any questions? Please call us at 407-876-6226 or e-mail us GENESIS@MAGICNET.NET. ****************************************************************************** From: genesis@magicnet.net (Val Taylor) Date: Fri, 28 Feb 1997 20:17:28 GMT Subject: WWW.TECNET.COM - GLOBAL USED EQUIPMENT DATABASE Organization: Genesis Scientific/Tecnet Your one stop hi-tech used equipment source is at WWW.TECNET.COM. We specialize in electrical, semiconductor, optical, and mechanical engineering (production, test, and laboratory) equipment. Whether your expanding your lab, replacing a piece of equipment, or opening a new research/production based business, we got all from office furniture and equipment to lasers and wafer handlers. We will also list your surplus assetts on our database for free! Any questions? Please call us at 407-876-6226 or e-mail us GENESIS@MAGICNET.NET. ****************************************************************************** From: "James Duckworth" Date: 1 Mar 1997 02:45:59 GMT Subject: Re: to HP MS-ChemStation 3.x (Pascal) Users Organization: AT&T WorldNet Services bernd alexander walter gallasch wrote in article <5f410e$c02@acmey.gatech.edu>... } To all Users o the pascal software for the HP MS-ChemStation 3.x } } I'am using a HP 9000/300 with MS-ChemStation 3.2 and need to transfer } files onto DOS or Linux formatted diskettes. Does somebody have } a program either for the pascal computer to save chromatograms and } spectra on DOS or Linux formatted diskettes or a program for DOS or } Linux platforms to read pascal formatted diskettes? Please answer to: } bernd.gallasch@uni-bayreuth.de } } } I seem to recall a company here in the states named "Oswego Software". or something like that. They sold a program that allowed a PC disk drive to read the HP Pascal formatted disks and dump the data files onto a DOS formatted disk. The reason I know this is that my company used this software then wrote a conversion utility to read the MSD files into our commercial Windows product, GRAMS/32. I also remember that Infometrix in out in Seattle, WA makes (or used to make) a hardware link for a PC to pull data off the PAWS computers. We never tried this though. Unfortunately, I'm not in my office right now, but at home so I don't have all the information, in front of me. But if you're actually still interested, just drop me an e-mail at JHD@galactic.com (don't respond to this message, I don't check the mailbox very often on this account). James Duckworth Galactic Industries Corp. ****************************************************************************** From: Gary Radford Date: Sat, 01 Mar 1997 22:32:05 -0500 Subject: Re: HP MSD Datasystem Organization: Radford Scientific Ron_Cochran@EMAIL.JRC.COM wrote: } We have an HP 5971A MSD for which the data system runs on an HP PC } Clone under Windows. } Can someone tell me a way to get chromatogram or spectral plots to the } standard Windows clipboard, so that they can be dealt with by some } other Windows software - like Powerpoint, for example? My goal is to be } able to annotate such plots easily. I am not aware that we can currently } do such annotation under our HP software. } } Thank you! } } R.L. Cochran } James River Corp. } Ron_Cochran@email.jrc.com Without knowing the version number you should be able to try 'Copy Window' in the Options menu to send a displayed chromatogram to the Windows Clipboard...then Paste in Powerpoint Garyr ****************************************************************************** From: Volker Haeupl <100277.713@CompuServe.COM> Date: 2 Mar 1997 22:14:46 GMT Subject: Re: HP MSD Datasystem Organization: It's just me ... What version of the ChemStation SW do you have? It might be that you have an Options menu in your Data Analysis SW where you find the item "Copy Window..." which is exactly what you are looking for. Regards, Volker / 100277.713@compuserve.com ****************************************************************************** From: tswenson@mindspring.com (Tom) Date: Sun, 02 Mar 1997 19:40:49 -0700 Subject: Remanufactured GC and GC/MS Organization: SpectraSource SpectraSource, Inc. sells remanufactured analytical instruments, specifically Hewlett-Packard GC, GC/MS and HPLC and Perkin-Elmer AA, GFAA and ICP, plus accessories and peripherals. More information at: http://www.spectra-source.com or call 919.571.0940. Look for us at Pittcon '97 in Atlanta, too, March 17-20 ****************************************************************************** From: y2-sano@eisai.co.jp (Yoshihisa Sano) Date: Mon, 3 Mar 1997 10:41:31 +0900 Subject: Re>>XSQ7000 heated capillary Organization: * "Tom Cromartie" wrote in article }Yoshihisa, }I've used similar solvents for 2 years with no problem. However I rarely }use TFA, I usually use HCO2H or HOAc. Still I doubt TFA should have }caused your problem......... Bruce, Thank you for your kindly responding to our question about the heated capillary. I have similar comments on it from other users/engineers directly responded via e-mail. We can now conclude that TFA in the mobile phase of HPLC may harm the instrument during continuous quantitative analysis for a long period (more than 24 hours), and we should replace TFA by other moderate buffers such as AcOH and HCOOH, although we sometimes need TFA as a component of mobile phase to achieve better separation of interests on HPLC and/or MS chromatograms. We finally hope that we can use many kinds of buffers for HPLC mobile phases with minimum disadvantages in the near future. Best regards, Yoshihisa Sano Drug Metabolism Research Section, Eisai Co., Ltd., Tsukuba, Ibaraki, 300-26 Japan (Mar. 3, 1997) Yoshihisa Sano ****************************************************************************** From: "Steven P. Cepa 847-937-7539" Date: Wed, 26 Feb 1997 08:27:00 -0600 (CST) Subject: Meeting reminder Organization: * Deadline for registering for the March MCM-MSDG meeting is Thursday March 6. Meeting: Tuesday, March 11, 1997 Time: Social hour @ 5:30 Dinner @ 6:15 Talk @ 7:15 Cost: $22.00 Speaker: Robert Voyksner Research Triangle Institute Topic: Advances in LC/MS & CE/MS Using Ion Trap Mass Spectrometry Registration: 630-603-5579 (leave voice-mail message) (there is a typo in the Newsletter published number) ****************************************************************************** From: walther@servier.fr (Ph. VAYER) Date: 3 Mar 1997 08:50:44 GMT Subject: API fragmentation Organization: servier.fr Hello, I would like to know is somebody has erad about a piece of software which can give some information in the fragmentation induced by low energy collision (API source or QQQ collision cells). Thank you in advance, Ph. VAYER ****************************************************************************** From: Stanton Date: Mon, 03 Mar 1997 08:12:20 -0500 Subject: Re: to HP MS-ChemStation 3.x (Pascal) Users Organization: I SPEAK ONLY FOR MYSELF James Duckworth wrote: } } bernd alexander walter gallasch wrote in } article <5f410e$c02@acmey.gatech.edu>... } } To all Users o the pascal software for the HP MS-ChemStation 3.x } } } } I'am using a HP 9000/300 with MS-ChemStation 3.2 and need to transfer } } files onto DOS or Linux formatted diskettes. Does somebody have } } a program either for the pascal computer to save chromatograms and } } spectra on DOS or Linux formatted diskettes or a program for DOS or } } Linux platforms to read pascal formatted diskettes? Please answer to: } } bernd.gallasch@uni-bayreuth.de } } } } } } } } I seem to recall a company here in the states named "Oswego Software". or } something like that. They sold a program that allowed a PC disk drive to } read the HP Pascal formatted disks and dump the data files onto a DOS } formatted disk. The reason I know this is that my company used this } software then wrote a conversion utility to read the MSD files into our } commercial Windows product, GRAMS/32. } } I also remember that Infometrix in out in Seattle, WA makes (or used to } make) a hardware link for a PC to pull data off the PAWS computers. We } never tried this though. } Yes, the Oswego Software enables the Pascal Workstation to write a DOS formatted floppy diskette. Oswego Software's phone number is (708) 554-3567. InfoMetrix's MasterLink allows a serial transfer between the HP 9000/300 and a PC. Their number is (206) 441-4696. If you need to convert between actual formats (e.g. to migrate GC/MS data & library files between different data systems) our software MASSTransit performs quite a few different conversions. -Stanton -- _____________________________________________________________________ Stanton Loh, Ph.D. Palisade Corporation Product Mgr., Mass Spectrometry 31 Decker Road mailto:sloh@palisade.com Newfield, NY 14867 http://www.palisade.com 607.277.8000 (8001 fax) ****************************************************************************** From: "Dr. Gregory G. Dolnikowski" Date: Mon, 03 Mar 1997 10:03:48 -0500 Subject: Re: HP MSD Datasystem Organization: Tufts University Ron_Cochran@EMAIL.JRC.COM wrote: } } We have an HP 5971A MSD for which the data system runs on an HP PC Clone } under Windows. } } Can someone tell me a way to get chromatogram or spectral plots to the } standard Windows clipboard, so that they can be dealt with by some other } Windows software - like Powerpoint, for example? My goal is to be able to } annotate such plots easily. I am not aware that we can currently do such } annotation under our HP software. } } Thank you! } } R.L. Cochran } James River Corp. } Ron_Cochran@email.jrc.com If a Chemstation chromatogram or spectrum is displayed on the screen, one can easily create a bitmap picture of it by Hitting the PrintScreen button on your PC keyboard and then entering Powerpoint to paste the clipboard image into a presentation. This approach is limited to the resolution of your monitor. If you have a high resolution monitor this will probably be sufficient for most presentations. Another approach is to import the data file itself into GRAMS, a software package from Galactic Industries. One can annotate the spectra or chromatograms directly in GRAMS or one can save them as metafiles which can be imported into Powerpoint. Metafiles are vector representations of the data and are not limited to screen resolution. I use both of these approaches on a routine basis. Gregory G. Dolnikowski Mass Spectrometry Lab Chief Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University 711 Washington Street Boston MA 02111 (617) 556 3298 dolnikows_ms@hnrc.tufts.edu ****************************************************************************** From: charles metcalfe Date: Mon, 03 Mar 1997 23:01:32 -0500 Subject: Fragrance MS Database Organization: Voicenet - Internet Access - (215)674-9290 I am a current user of an HP GC-MS system. I would like to know where I can obtain or purchase a database that specializes in aroma and fragrance compounds. I would also consider a book of reference spectra. thanx ****************************************************************************** From: "Greg R. Shaw" Date: Mon, 03 Mar 1997 10:09:08 -0800 Subject: Finnigan 4500 For Sale Organization: West Coast Analytical Service For sale: Finnigan 4500 quadrupole GC/MS/DS. 2-1024 mass range, 9610 GC, dual diffusion pump, solids probe, -3kV dynode, Incos Rev 6.5 software (including 42000 entry NIST library), Printronix MVP printer, 70 Mb Winchester drive, Cipher streamer tape. Includes user and service manuals, full schematics. Please Contact: eric.lindsay@wcaslab.com -- Eric Lindsay West Coast Analytical Service eric.lindsay@wcaslab.com 9840 Alburtis Avenue Phone: (562) 948-2225 Santa Fe Springs, CA 90670 Fax : (562) 948-5850 http://www.wcaslab.com ****************************************************************************** From: R.D.vanOoijen@FAR.RUU.NL (R.D.van Ooijen) Date: Wed, 05 Mar 1997 00:54:25 GMT Subject: High gasflow in MSD Organization: University of Utrecht Faculty of Pharmacy We are using an HP -MSD combination with a 5790A GLC and a 5970A msd Now we have the problem that we get a high gas flow of the carrier gas. This is so high that we have trouble to maintain the vacuum. Hass anybody there an idea how we can solve the problem. For info, we have already cleaned all the gas tubes, from the split and septum wash. Also the needle ventiles are cleaned. So is the inlay at the bottom of in the injection port. The manometer is ok. Even when we slow down the pressure on the system we have high flow. Thanks in advance Ronald van ooijen ****************************************************************************** From: "H. K. Wang" Date: Tue, 04 Mar 1997 11:54:23 -0800 Subject: About price rate Organization: UNC-Chapel Hill To University Mass experts: Hi, We just got a VG7070E Mass spectrometer and want to set up a price schedule. I want to get a general price range in universities of USA. How much do you charge for EI, CI or FAB per sample? Thanks for your attention. HK WANG, Ph.D. Research Associate Professor Room 302, Beard Hall School of Pharmacy Univ. of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7360 Tel: 919-966-5908 FAX: 919-966-3893 Email: hwang@gibbs.oit.unc.edu ****************************************************************************** From: Date: 4 Mar 1997 17:48:09 GMT Subject: Best MS config for peptides? Organization: Sprynet News Service To analyze/characterize peptides, proteins and/or for general pharmaceutical research, can an HP 5989 be configured with LC to serve this purpose well? Is it important to have extended mass range (2000 amu vs standard 1000 amu)? Is API essential? If so, is AP-ES or AP-CI better, and how much worse is the worse of the two? How would particle beam and thermospray compare with API for these purposes? Last but not least, is the 5989B significantly superior to the 5989A? Any suggestions for any other LC/MS system that might be old enough to be on the used market that will serve these purposes would also be greatly appreciated. ****************************************************************************** From: cato.brede@kjemi.uio.no (Cato Brede) Date: 4 Mar 1997 18:10:50 GMT Subject: You have: Vestec LC-MS Organization: University of Oslo, Norway Hello If you have a Vestec LC-MS instrument, please do me a favour and send the settings on your Edwards Controller 1105 (black box in front of the instrument). I suspect that my settings are corrupted and need to be checked. The manuals are not very helpful in this matter. You can do this easily (2 min) by pressing the S (with a ring). Then go up or down with the arrows. After reading off the settings, press S to return. Please note the settings and e-mail me. Thanks. 4 values for low settings (arrow down in front of set-point) Gauge# (right number): Pressure (i.e. Torr): 4 values for high settings (arrow up after set-point) Gauge# (right number): Pressure (i.e. Torr): Sincerely Cato Brede ****************************************************************************** From: Stefan Knecht Date: Tue, 04 Mar 1997 18:24:16 +0100 Subject: Massenspektrum Organization: Heinrich Heine Universitaet Duesseldorf Wer kann mir einen kleine Hilfe fuer die Auswertung des folgenden Massenspektrums geben? Das Spektrum koennt Ihr euch unter http://www-public.rz.uni-duesseldorf.de/~knecht/download/spektrum.htm anschauen. Es handelt sich um eine org. Verbindung mit den moeglichen Heteroatomen N, S, O, Cl, Br, I, F. Alle Heteroatome bis auf O habe ich jedoch schon durch Betrachtung der m/e ausgeschlossen. Leider bin ich mir auch bei der Zuordnung des Molekuelionenpeaks noch nicht ganz sicher. Der charakteristischte Peak des Spektrums liegt bei m/e=43, was fuer Acetyl als Folge einer McLafferty Umlagerung bei Ketonen sprechen wuerde. Wuerde es sich um ein Keton handeln, so wuerden die entstehenden Carbonylbruchstuecke leicht CO (m/e 28)abspalten, was zu einem Molekuelionenpeak bei 100 bzw. 101 fueren wuerde. Ein Indiez fuer die Anwesenheit von O soll laut Literatur die Wasserabspaltung sein. Demnach muesste die Masse des Molekuelionenpeaks um 18 abnehmen. Weder fuer 100 noch fuer 101 gibt es ein Fragment mit einer um 18 kleineren Masse. Deshalb meine Frage. Welches ist der Molekuelionenpeak? Wenn er bei 100 bzw. 101 liegt, wie kann man dann das Nichtauftreten der Peaks bei 82 bzw. 83 (Wasserabspaltung) erklaeren. Vielen Dank fuer Eure Hilfe Stefan ****************************************************************************** From: Stefan Knecht Date: Tue, 04 Mar 1997 22:05:57 +0100 Subject: Mass spectrum Organization: Heinrich Heine Universitaet Duesseldorf [We requested an English translation of the previous article, and have posted both of them. DB] Can You help me to interpret the following mass spectrum? You can find the spectrum at http://www-public.rz.uni-duesseldorf.de/~knecht/download/spektrum.htm It shoud be an organic compound with possible hetero atoms like N, S, O, Cl, Br, I, F. I eliminated all atoms expect of O (Concerning to possible fragmentations). I have some difficulties in finding the peak of the molecule ion. The characteristic peak of this spektrum is m/e 43, which indicates acetyl as a consequence of a McLafferty rearrangement of fragment ions of ketones. If the compound would be a ketone, the fragments should loose CO (m/e 28), what indicates a molecule ion peak at 100 or 101. Reffering to literature, a characteristic reaction for the presence of O in the compound should be the elimination of water. As a result the mass of the molecule-ion must be reduced by 18. But there exists no fragment with a mass of 82 or 83 concerning to a molecule-ion at 100 or 101. So, can You tell me which peak indicates the molecule-ion peak? Sorry about this bad English, but it's the first time I write a chemical article in English. Your's Stefan ****************************************************************************** From: bigcheez@ma.ultranet.com Date: Tue, 04 Mar 1997 23:21:13 -0600 Subject: For Sale: MALDI-TOF excellent condition, $90,000 Organization: UltraNet Communications, Inc. We have several delayed extraction MALDI-TOF systems for sale, complete with pentium computer, color printer and software. Available on a first come basis. ****************************************************************************** From: Gary Radford Date: Tue, 04 Mar 1997 23:09:33 -0500 Subject: Re: High gasflow in MSD Organization: Radford Scientific R.D.van Ooijen wrote: } } We are using an HP -MSD combination with a 5790A GLC and a 5970A msd } Now we have the problem that we get a high gas flow of the carrier gas. } This is so high that we have trouble to maintain the vacuum. } Hass anybody there an idea how we can solve the problem. } I guess I would ask you to check 2 things for me 1. Measure the flow comming out of the rough pump exit......it should be less than 1 ml/min with the column installed. 2. Remove the column from the injection port and plug the end of the colmn with a good septum....the flow should be zero in about 3 to 5 minutes If either of the above tests fail then you probably have a leak in the MSD . } For info, we have already cleaned all the gas tubes, from the split and } septum wash. Also the needle ventiles are cleaned. So is the inlay at } the bottom of in the injection port. The manometer is ok. } } Even when we slow down the pressure on the system we have high flow. } } Thanks in advance } } Ronald van ooijen Hope this helps to identify wether this is a GC or MS problem. Gary ****************************************************************************** From: bernd alexander walter gallasch Date: Wed, 05 Mar 1997 12:10:48 +0000 Subject: Re: High gasflow in MSD Organization: Uni-Bayreuth, Arbeitskreis Spiteller R.D.van Ooijen wrote: } } We are using an HP -MSD combination with a 5790A GLC and a 5970A msd } Now we have the problem that we get a high gas flow of the carrier gas. .... Hi Ronald, I don't know why your system works earlier, but to reduce your gasflow you have to connect a thin transfer capillary to your MSD. The best results gave capillaries with 0.15 to 0.20 µm ID. I purchase mine from Gerstel GmbH, Aktienstr. 232-234, D-45473 Mülheim an der Ruhr, Postfach 100626, Tel: +49(0)208 7 65 03-0, fax: +49(0)208 7 65 03 33, internet: http://www.gerstel.com 10 m of 0.2 µm ID costs about 420.- DM. You can purchase transferlines too from SGE. You will find your next office at http://www.sge.com/offices.html. I hope it will help you, Bernd. ****************************************************************************** From: Frank_Hollis-1@sbphrd.com.see-sig (Triple Quadrophenic) Date: 5 Mar 1997 11:12:17 GMT Subject: Re: API fragmentation Organization: SB In article <5feils$9jk@acmex.gatech.edu>, walther@servier.fr (Ph. VAYER) dusted off the quill, prised open the inkwell and wrote... } } }Hello, } }I would like to know is somebody has erad about a piece of software which }can give some information in the fragmentation induced by }low energy collision (API source or QQQ collision cells). } I very much doubt that such software exists. Collision induced fragmentation is notoriously difficult to predict and interpret. Often you'll find that fragments are obvious. However, a very small change to the structure of the molecule will change the fragmentation to something that is a complete mystery. But... If anybody's got such a piece of software..... -- Frank J Hollis, Mass Spectroscopy, SmithKline Beecham, Welwyn, UK Frank_Hollis-1@sbphrd.com or fjh4@tutor.open.ac.uk http://www.geocities.com/CapeCanaveral/Lab/1550/ These opinions have not been passed by 3 committes, 7 subcommittees and continuous improvement teams. So they can't be the opinions of my employer. ****************************************************************************** From: Bill Cotham Date: Wed, 05 Mar 1997 09:38:47 -0500 Subject: Re: Best MS config for peptides? Organization: USC Mass Spectrometry Center sherrell@sprynet.com wrote: } } To analyze/characterize peptides, proteins and/or for general } pharmaceutical research, can an HP 5989 be configured with LC to } serve this purpose well? Yes it can be configured for LC-MS. } Is it important to have extended mass range (2000 amu vs } standard 1000 amu)? Very important to have extended mass range, esp. if analyzing intact proteins. } Is API essential? If so, is AP-ES or AP-CI better, and how much } worse is the worse of the two? For running peptides and proteins, electrospray is essential. ES will in many cases also ionize many small polar organic molecules. If possible, I would try to have both APCI and ES capabilities. The APCI can tackle some of the more nonpolar molecules that are not amenable to ES. } How would particle beam and thermospray compare with API for } these purposes? Thermospray is probably inferior to APCI for most compounds and really should not be compared to ES. Particle beam produces library searchable spectra but is not very sensitive. Both thermospray and particle beam will not work for peptides and proteins. } Last but not least, is the 5989B significantly superior to the 5989A? } Any suggestions for any other LC/MS system that might be old enough } to be on the used market that will serve these purposes would also be } greatly appreciated. I don't know about the differences from the A to the B. I have occasionally seen older Finnigan SSQs on the market which might suit your needs. -- Bill Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry and Biochemistry University of South Carolina Columbia, SC 29208 803-777-2039 (voice) 803-777-9521 (fax) ****************************************************************************** From: Gary Lavigne Date: Wed, 05 Mar 1997 09:44:00 -0500 Subject: Re: Mass spectrum Organization: University of Connecticut To Stefan Knecht The Mass Spectrum may be 2-Pentanone,3,4-epoxy- which is a ketone compound as you suggested. Synonym = Ethanone,1-(3-methyloxiranyl)- CAS# 17257-79-3 Gary Lavigne } } [We requested an English translation of the previous article, and have } posted both of them. DB] } } Can You help me to interpret the following mass spectrum? You can find } the spectrum at } http://www-public.rz.uni-duesseldorf.de/~knecht/download/spektrum.htm } cut ****************************************************************************** From: Bill Cotham Date: Wed, 05 Mar 1997 10:37:01 -0500 Subject: Re: Mass spectrum Organization: USC Mass Spectrometry Center Stefan Knecht wrote: } } [We requested an English translation of the previous article, and have } posted both of them. DB] } } Can You help me to interpret the following mass spectrum? You can find } the spectrum at } http://www-public.rz.uni-duesseldorf.de/~knecht/download/spektrum.htm } } It shoud be an organic compound with possible hetero atoms like N, S, O, } Cl, Br, I, F. I eliminated all atoms expect of O (Concerning to possible } fragmentations). } } I have some difficulties in finding the peak of the molecule ion. The } characteristic peak of this spektrum is m/e 43, which indicates acetyl } as a consequence of a McLafferty rearrangement of fragment ions of } ketones. If the compound would be a ketone, the fragments should loose } CO (m/e 28), what indicates a molecule ion peak at 100 or 101. Reffering } to literature, a characteristic reaction for the presence of O in the } compound should be the elimination of water. As a result the mass of the } molecule-ion must be reduced by 18. But there exists no fragment with a } mass of 82 or 83 concerning to a molecule-ion at 100 or 101. } } So, can You tell me which peak indicates the molecule-ion peak? I think mw is 100. The spectrum is consistent with 2,4 pentanedione. -- Bill Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry and Biochemistry University of South Carolina Columbia, SC 29208 803-777-2039 (voice) 803-777-9521 (fax) ****************************************************************************** From: Keith Martinko Date: Wed, 05 Mar 1997 10:03:33 -0600 Subject: Re: Fragrance MS Database Organization: ProLab Resources, Inc. charles metcalfe wrote: } } I am a current user of an HP GC-MS system. I would like to know where I } can obtain or purchase a database that specializes in aroma and } fragrance compounds. I would also consider a book of reference spectra. } There is a database developed by Robert Adams at Baylor University that contains over 1200 of the most commonly occurring compounds found in plant essential oils. This library database is available in the HP ChemStation (.L) format and can be purchased from : Allured Publishing Corp. 362 S. Schmale Road Carol Stream, IL 60188 USA 630-653-2155 630-653-2192 Essential Oils by Gas Chromatography and Mass Spectrometry - Computer Mass Spectral Library ($395 + shipping) (3.5" diskettes) We have a number of customers in the flavor/fragrance industry on our MS data system and they have found it to be "essential". Hope this helps ... Keith Martinko ProLab Resources, Inc. prolab@ix.netcom.com http://www.prolab.com ****************************************************************************** From: Bill Cotham Date: Wed, 05 Mar 1997 11:18:49 -0500 Subject: Re: Mass spectrum Organization: USC Mass Spectrometry Center Gary Lavigne wrote: } } To Stefan Knecht } The Mass Spectrum may be 2-Pentanone,3,4-epoxy- which is a ketone } compound as you suggested. } Synonym = Ethanone,1-(3-methyloxiranyl)- } CAS# 17257-79-3 } Gary Lavigne } Gary, My library spectrum of the above compound lacks a molecular ion (100 amu). The spectrum in question has a peak at 100. I think it is 2,4 pentanedione. -- Bill Cotham, Ph.D. Mass Spectrometry Laboratory Dept. of Chemistry and Biochemistry University of South Carolina Columbia, SC 29208 803-777-2039 (voice) 803-777-9521 (fax) ****************************************************************************** From: B.Hamilton@irl.cri.nz Date: Wed, 05 Mar 1997 15:12:46 GMT Subject: Re: MS Chemstation data export II Organization: Industrial Research Limited Kjetil Jorgensen wrote: }Thank you for your answers to my first question "MS Chemstation data }export". However I guess I was not precise enough presenting my problem. } }I have a HP MSD Chemstation SW (currently upgrading to 1701 v. 3.00). Yes, I wondered if it was an MSD version. I've cross-posted this to sci.techniques.mass-spec. I'm only familiar with ordinary GC and HPLC Chemstations. }I would like to export the complete - or selected parts - of the raw }data matrix to ASCII format or any other format readable by excel. Have you asked if there is a "user-contributed, unsupported routines disk ", as there are for the Microsoft Windows versions of GC and LC Chemstation. They have several routines. It also may be possible to export to a standard data interchange format from your new version, and then use other software to convert to Excel. }I have contacted HP here in Norway. They could not help me, and }recomended me to write a macro by myself. Since I don't have any }experience with HP macros or don't know anything about the data format }that HP use for their MS files I would appreciate it if someone out }there may give som clues for how to solve my problem. Bruce Hamilton ****************************************************************************** From: Gary Lavigne Date: Wed, 05 Mar 1997 14:46:15 -0500 Subject: Re: Mass spectrum Organization: University of Connecticut Correction to previous post 2,4 Pentanedione is the correct match. Sorry I missed it on the first post. Gary Lavigne } } To Stefan Knecht } The Mass Spectrum may be 2-Pentanone,3,4-epoxy- which is a ketone } compound as you suggested. } Synonym = Ethanone,1-(3-methyloxiranyl)- } CAS# 17257-79-3 } Gary Lavigne } } } } cut ****************************************************************************** From: Sung-Chan Jo Date: Thu, 06 Mar 1997 10:49:34 -0800 Subject: Re: Mass spectrum Organization: SKC R&D Center Stefan Knecht wrote: } } Can You help me to interpret the following mass spectrum? You can find } the spectrum at } http://www-public.rz.uni-duesseldorf.de/~knecht/download/spektrum.htm } } It shoud be an organic compound with possible hetero atoms like N, S, O, } Cl, Br, I, F. I eliminated all atoms expect of O (Concerning to possible } fragmentations). I think you must have wanted to write 'except' instead of 'expect'. Am I right ? Yeah, you were right. The spectrum shows it has no other heteroatoms except O. } The characteristic peak of this spektrum is m/e 43, which indicates acetyl } as a consequence of a McLafferty rearrangement of fragment ions of } ketones. You were right partly in that it has acetyl group. But you have something to know about the rest of it. McLafferty rearrangement from molecular ion produces only Odd Electron Ion. So if the compound has no nitrogen, the fragmentation should give an ion of even mass, not of odd mass like m/e=43. } If the compound would be a ketone, the fragments should lose } CO (m/e 28), what indicates a molecule ion peak at 100 or 101. You can see the very peak at m/e=72, can't you ? That is an evidence that m/e=100 peak is the molecular ion. } Reffering to literature, a characteristic reaction for the presence of O in } the compound should be the elimination of water. As a result the mass of the } molecule-ion must be reduced by 18. Not in the case. I think you must have read it from a text on CI technique. That is not always applicable to mass spectra. It is kind of unqualified generalization. Let's summarize all aboves. The spectrum has Molecular ion peak at m/e=100. And the compound is 2,4-Pentanedione. The peak at m/e=43 says the sample has acetyl group, of course. But you missed the relative abundances of prominent peaks such as m/e=43, 85, 100. In case of ketones, loss of methyl gourp is unusual except additional stabilization is achieved for the resulting ion. That was the key. Hope it helps, Regards Sung-Chan Jo ****************************************************************************** From: markusg@hal2000.chemie.uni-mainz.de (Markus Gerle) Date: Thu, 06 Mar 97 17:57:17 GMT Subject: MALDI on olefine-oligomers Organization: university of mainz Dear all! Does anyone of you have experience on MALDI-TOF measurements of olefines? Any informations concerning matrix-composition an so on would be helpful. Thank´s in advance and best regards Markus Gerle ****************************************************************************** From: pggreen@cco.caltech.edu (Peter G. Green) Date: Thu, 6 Mar 97 16:00:11 GMT Subject: Re: High gasflow in MSD Organization: Caltech (Environmental Engineering Science) }I guess I would ask you to check 2 things for me } 1. Measure the flow comming out of the rough pump exit......it }should be less than 1 ml/min with the column installed. } } 2. Remove the column from the injection port and plug the end of the }colmn with a good septum....the flow should be zero in about 3 to 5 }minutes } }If either of the above tests fail then you probably have a leak in the }MSD . } Couldn't #2 also be a big crack in the column? Perhaps plug at the inlet to the MS to distinguish between GC and MS. Should zero even faster. ****************************************************************************** From: "Nicholas A. Giorgio" Date: Fri, 7 Mar 1997 00:40:47 GMT Subject: purchasing a MALDI-TOF Organization: ImClone Systems I want to purchase a MALDI-TOF MS for the laboratory. We would use it to for QC of synthetic peptides, QC for synthetic oligos, screening column fractions during protein purification and to characterize protein-protein interactions. Our size limits are 1,000D - 175,000D. We have assayed samples on the KRATOS II ($95,000) and the Perceptive Voyager $115,000, demo model. The latter had slightly better resolution. What would you buy? ****************************************************************************** From: Hartmut Doenges Date: Fri, 07 Mar 1997 08:47:07 +0100 Subject: Re: to HP MS-ChemStation 3.x (Pascal) Users Organization: Hewlett-Packard bernd alexander walter gallasch wrote: } } To all Users o the pascal software for the HP MS-ChemStation 3.x } } I'am using a HP 9000/300 with MS-ChemStation 3.2 and need to transfer } files onto DOS or Linux formatted diskettes. Does somebody have } a program either for the pascal computer to save chromatograms and } spectra on DOS or Linux formatted diskettes or a program for DOS or } Linux platforms to read pascal formatted diskettes? Please answer to: } bernd.gallasch@uni-bayreuth.de -- HP provides a utility to read pascal formatted floppy disks on a DOS platform. The tool is called "LIF utility". Please contact your local support to get it. -- ****************************************************************************** From: GRAEME ROBERTSON Date: Fri, 07 Mar 1997 11:49:16 -0800 Subject: Re: Fragrance MS Database Organization: SCOTTISH CROP RESEARCH INSTITUTE charles metcalfe wrote: } } I am a current user of an HP GC-MS system. I would like to know where I } can obtain or purchase a database that specializes in aroma and } fragrance compounds. I would also consider a book of reference spectra. } } thanx Try QUALITATIVE ANALYSIS OF FLAVOUR AND FRAGRANCE VOLATILES BY GLASS CAPILLARY GAS CHROMATOGRAPHY. by WALTER JENNINGS AND TAKAYUKI SHIBAMOTO. ACADEMIC PRESS. 1980, its slightly old now but good fundamental text ISBN 0-12-384250-6 G W ROBERTSON SCRI DUNDEE SCOTLAND -- GRAEME ROBERTSON MASS SPECTROMETRY UNIT/DEPT OF CHEMISTRY SCOTTISH CROP RESEARCH INSTITUTE INVERGOWRIE, DUNDEE,SCOTLAND ****************************************************************************** From: heino.prinz@mpi-dortmund.mpg.de (Heino Prinz) Date: Fri, 07 Mar 1997 14:25:41 GMT Subject: Adduct of proteins at mass 96? Organization: GWDG, Goettingen Hallo Everyone, Doing ESI-MS of proteins, I have often encountered an adduct of mass 96 to the expected molecular weight. These proteins have all been expressed in E.Coli and the people involved say that TFA ( likely candidate for such an adduct) had never been used at any stage with these proteins. Does anybody have an idea regarding the nature of these adducts? Any suggestions would be welcome Yours sincerely, Heino Prinz Tel.:(+49)-231-1206-332 Fax:(+49)-231-1206-389 ****************************************************************************** From: sumner@chemvx.chem.tamu.edu (Sumner Lloyd) Date: Fri, 7 Mar 1997 09:26:52 -0600 Subject: Edward Gauge Controller Organization: * Cato Brede wrote: }Hello }If you have a Vestec LC-MS instrument, please do me a favour and send the }settings on your Edwards Controller 1105 (black box in front of the }instrument). I suspect that my settings are corrupted and need to be }checked. }The manuals are not very helpful in this matter..................... }Sincerely }Cato Brede Cato, We have a Vestec 201A LC-MS fitted with both thermospray and electrospray. The 201A is equipped witn an Edwards 1105 gauge controller and the requested settings are as follows: LOW HIGH #1 7.0x10-2 torr 3.0x10-1 torr #2 1.2x10-1 torr 5.0x10-1 torr #3 5.5x10-3 torr 9.0x10-3 torr #4 5.0x10-1 torr 5.5x10-1 torr Good luck on your problems, Lloyd W. Sumner, Ph.D. Associate Director, The Laboratory for Biological Mass Spectrometry Department of Chemistry Texas A&M University College Station, TX 77843-3255 Voice #(409)845-8404 Facsimile#(409)845-4719 Email Sumner@chemvx.tamu.edu . "The world is divided into people who do things - and people who get the credit." -Dwight Morrow ****************************************************************************** From: winterconf@chem.umass.edu (Ramon.M.Barnes) Date: Sun, 9 Mar 1997 10:12:33 -0500 Subject: 1998 Winter Conference (LONG) Organization: * Dear Colleagues, Here is the latest information concerning the 1998 Winter Conference. Please circulate it to your colleagues and potential participants in the next Winter Conference. We hope that you will join us too. Later this month or early next month we will have available a complete conference registration package, that will contain a preliminary program, abstract/title submission forms, conference and hotel registration materials, lists of short courses, and details of travel and hotel accommodations. Information for exhibitors, promotions, and advertising also will be available later this month. Please let me know if you would like to receive these packages. 1998 Winter Conference on Plasma Spectrochemistry Scottsdale, Arizona, January 5 - 10, 1998 The 1998 Winter Conference on Plasma Spectrochemistry, tenth in a series of biennial meetings sponsored by the ICP Information Newsletter, features developments in plasma spectrochemical analysis by inductively coupled plasma (ICP), dc plasma (DCP), microwave plasma (MIP), and glow discharge (GDL, HCL) sources. The meeting will be held Monday, January 5 through Saturday, January 10, 1998, in Scottsdale, Arizona at the DoubleTree Paradise Valley Resort. Continuing education short courses at introductory and advanced levels will be offered Friday through Sunday, January 2 - 4. Spectroscopic instrumentation and accessories will be shown during a three-day exhibition. A gold tournament will be held on Sunday, January 4. Objectives and Program The continued growth in popularity of plasma sources for atomization and excitation in atomic spectroscopy and ionization in mass spectrometry and the need to discuss recent developments of these discharges in spectrochemical analysis stimulated the organization of this meeting. The Conference will bring together international scientists experienced in applications, instrumentation, and theory in an informal setting to examine recent progress in the field. More than 500 participants from 25 countries are expected to attend. Approximately 300 papers describing applications, fundamentals, and instrumental developments with plasma sources are expected to be presented in lecture and poster sessions by more than 200 authors. Symposia organized and chaired by recognized experts will include the following topics: 1) Sample introduction and transport phenomena; 2) Flow injection and flow processing spectrochemical analysis; 3) Elemental speciation with plasma/chromatographic techniques; 4) Plasma instrumentation, including chemometrics, expert systems, on-line analysis, software, and remote-system automation; 5) Sample preparation, treatment and automation; 6) Excitation mechanisms and plasma phenomena; 7) Spectroscopic standards and reference materials, high-purity materials; 8) Plasma source mass spectrometry; 9) Glow discharge atomic and mass spectrometry; 10) Applications of stable isotope analyses, and 11) Laser-assisted plasma spectrometry. Six plenary and 21 invited lectures will highlight advances in these areas. Four afternoon poster sessions will feature applications, automation, and new instrumentation. Five panel discussions will address critical development areas in sample introduction and preparation, instrumentation, elemental speciation, plasma source mass spectrometry, and novel software and hardware. Plenary, invited, and submitted papers will be published in Fresenius' Journal of Analytical Chemistry and Journal of Analytical Atomic Spectrometry in Fall 1998. Invited Speakers Invited speakers will include six distinguished plenary lecturers (M.B. Denton, R. Lobinski, D. Douglas, J. van der Mullen, D.Koppenaal, W. Harrison) and 21 leading plasma spectrochemists. Panel discussions and symposia also will be lead by noted analytical chemists. Instrument Exhibition A three-day exhibition of spectroscopic instrumentation and chemicals, electronics, glassware, publications, and software supporting plasma spectroscopy will complement the scheduled sessions on Tuesday through Thursday, January 6 - 8 with approximately 25 organizations participating in the 13,000 square foot Exhibition Forum. Continuing Education Short Courses Introductory and advanced, four-hour short courses will be presented Friday through Sunday, January 2 - 4. Designed to provide background and intensive training in popular topics of flow injection analysis and plasma spectrochemistry, more than 50 courses will be presented at 8 am to 12, 1 to 5 pm, or 7 to 11 pm. Social Activities The Conference will be held at the DoubleTree Paradise Valley Resort, a 22 acre site decorated in a southwestern style (see www.doubletreehotels.com) located only 10 miles (20 minutes) from the Sky Harbor International Airport in Phoenix and 2 to 4 blocks North of Historic Old Town Scottsdale and the Scottsdale Fashion Square. Scottsdale and the Valley of the Sun (Phoenix metropolitan area) combine internationally famous attractions, landmarks, museums, and entertainment in the desert mountains These include the Heard Museum, Phoenix Art Museum, Pueblo Grande Museum, Casa Grande Ruins, Desert Botanical Garden, Phoenix Zoo, Wildcat Park, Heritage Square, and Champlin Fighter Museum, South Mountain and Papago Parks. Local hiking includes Squaw Peak and Camelback Mountain. The Grand Canyon is less than 250 miles North (see www.arizonaguide.com). The maximum temperature in January is 65.9°F (18.9°C) and minimum of 41.2°F (5.1°C) with 78% possible sunshine (0.67 inches average precipitation, see www.intellicast.com/weather/phx/). The Paradise Valley Resort is a complete resort with two outdoor pools, two lighted tennis courts, three whirlpool spas, and a fitness center. Ten USGA golf courses are located within 4 miles, and horseback riding, ballooning, jeep tours and rafting are nearby. A Western evening January 8 will feature dinner and Native American and Western entertainment. Daily social hours and refreshments also are planned. Accommodations and Travel The Sky's the Limit Travel of Brattleboro, Vermont, is the official Conference travel agency. Accommodations at the Paradise Valley Resort, where all Conference activities will take place, can be reserved with Sky's the Limit Travel at a special Conference rate of $110 per day (excluding tax) before October 10, 1997. After that a late fee will be charged. A limited number of US-government-employee-rate rooms are available. Arrangements for families with children are provided, and extended stays before and after the Conference are offered at the Conference rate. Special low domestic and international airfares and discount automobile rentals are available exclusively through Sky's the Limit Travel. For travel information and reservations, contact Ray Beam, Sky's the Limit Travel at 800-336-4009 or 802-254-9180 (fax 802-254-8693), email skytrav@sover.net. Registration The Conference registration fee includes the Conference abstracts, Conference proceedings, and a souvenir tee shirt. The registration fee is $350 prior to October 10, $545 until December 5, and $660 thereafter. Discounts are provided for students, and no registration fee is required for spouses. The conference dinner is $45. Short-course preregistration fee is $80 prior to October 10, $145 until December 5, and $210 afterward for each four-hour course. For information concerning exhibition registration, facilities, and fees, contact the Conference chairman. Rates for the Conference program advertising, exhibition, and sponsored activities are available upon request. Further Information For further information, contact 1998 Winter Conference on Plasma Spectrochemistry, %ICP Information Newsletter, Department of Chemistry, LGRC Tower, University of Massachusetts, Box 34510, Amherst, MA 01003-4510 USA. ATTN: Dr. Ramon Barnes, Conference Chairman. Telephone (413) 545-2294, facsimile (413) 545-3757, e-mail winterconf@chem.umass.edu. 1998 Winter Conference on Plasma Spectrochemistry January 5 – 10, 1998, Scottsdale, Arizona Program Outline Monday, January 5, 1998 8 am OPENING AND WELCOME Ramon M. Barnes 8:05 (PL1) Plenary Lecture M. Bonner Denton, Spectroscopic Instrumentation in the 21st Century: Excitement on the Horizon 1. Sample Introduction and Transport Phenomena José A.C. Broekaert, Chairman 9:15 (IL1) Invited Lecture Akbar Montaser, Nebulizer Diagnostics: Fundamental Parameters, Challenges, and Tools 9:45 (IL2) Invited Lecture Richard F. Browner, Nebulizer Characteristics and Aerosols 2. Sample Introduction and Flow Processing Akbar Montaser, Chairman 1:00 (IL3) Invited Lecture John Olesik, Fundamental Processes Controlling ICP-OES and ICP-MS Signals 2:00 (IL4) Invited Lecture D. Conrad Grégoire, Analyte Transport in ETV-ICP-MS; Exploring the First Hyphen 5:30 (PD1) Panel Discussion Sample Introduction and Aerosol Transport: the End of the Road, Richard F. Browner, Chairman 6:30 Exhibition Opening and Social Mixer Tuesday, January 6, 1998 3. Elemental Speciation Olivier X. Donard, Chairman 8 am (PL2) Plenary Lecture Ryszard žobinski, Elemental Speciation and Coupled Techniques - Toward Faster and More Reliable Analysis. 9:15 (IL5) Invited Lecture Joseph A. Caruso, New Approaches to Elemental Speciation Studies with Chromatography/Plasma Mass Spectrometry 4. Elemental Speciation Joseph A. Caruso, Chairman 1:00 (IL6) Invited Lecture Les Ebdon, Benchmarking for Success. The Improvement of Speciation Analysis by the Application of Isotope Dilution Analysis 1:30 (IL7) Invited Lecture Alfredo Sanz-Medel, Elemental Speciation 3 - 6:30 Poster Session: Sample Introduction, Flow Injection Analysis, Elemental Speciation 5:00 Social Mixer 5:30 (PD2) Panel Discussion Challenges for Elemental Speciation, Olivier X. Donard, Chairman Wednesday, January 7, 1998 8 am (PL3) Plenary Lecture Donald Douglas, ICP-MS with Quadrupoles Operated in Alternative Stability Regions 5. Laser Assisted Plasma Spectrochemistry Gary M. Hieftje, Chairman 9:15 (IL8) Invited Lecture Jean-Michel Mermet, Status of Laser Ablation ICP Spectrometry 6. Plasma Instrumentation and Software Vassili Karanassios, Chairman 1:10 (IL9) Invited Lecture Gary Horlick, Plasma Mass Spectrometry: Are We Using the Right Source (revisited)? 1:40 (IL10) Invited Lecture Gary M. Hieftje, Plasma Spectrochemical Instrumentation for 2000 and Beyond 3 - 6:30 Poster Session: Automation, Instrumentation, Laser Assisted Plasma Spectrometry, Software 5:00 Social Mixer 5:30 (PD3) Panel Discussion Advances in Sample Preparation and Treatment, Isaac I. Brenner, Chairman Thursday, January 8, 1998 7. Excitation Mechanisms and Plasma Phenomena Jean-Michel Mermet, Chairman 8 am (PL4) Plenary Lecture J.A.M. van der Mullen, Fundamental Comparison Between ICP and MIP Discharges 9:15 (IL11) Invited Lecture Annemie Bogaerts and Renaat Gijbels, Glow Discharge Modelling 9:45 (IL12) Invited Lecture Mike Blades, The Need for Fundamental Measurements in the Development and Characterization of New Plasma Sources 8. Sample Preparation and Treatment for Plasma Spectroscopy, Analysis of High Purity Materials Skip Kingston, Chairman 1:10 (IL13) Invited Lecture Günter Knapp, Progress in Microwave Sample Decomposition by Means of Flow- and Batch-Systems 3 - 6:30 Poster Session: Applications, Glow Discharge Atomic and Mass Spectrometry, Mechanisms, Plasma Sources, Sample Preparation and Standards, Teaching Spectroscopy 4:30 (TS1) Panel Discussion Teaching, Training and Retraining in Spectroscopy, Vassili Karanassios, Chairman 6:30 Conference Western Cookout (Rawhide) Friday, January 9, 1998 9. Plasma Source Mass Spectrometry: Fundamentals, Instrumentation Gary Horlick, Chairman 8 am (PL5) Plenary Lecture David W. Koppenaal, G.C. Eiden, and C.J. Barinaga, Ion Traps and Collision Cells: Toward Interference-Free ICP/MS 9:15 (IL14) Invited Lecture R. Sam Houk, ICP-MS: Status and Future Instrumentation 9:45 (IL15) Invited Lecture Scott Tanner, Modern Plasma Source Mass Spectrometers 10. Plasma Source Mass Spectrometry: Applications Conrad Grégoire, Chairman 1:00 (IL16) Invited Lecture Klaus G. Heumann, Precision and Accuracy in Isotope Ratio Measurements by Plasma Source Mass Spectrometry 1:30 (IL17) Invited Lecture José Broekaert, Selected Examples of Speciation of Heavy Metals by Using ICP-MS for Elemental Detection 3 - 6:30 Poster Session: Plasma Mass Spectrometry, Applications, Fundamentals, Instrumentation, Stable Isotopes 5:00 Social Mixer 5:30 (PD4) Panel Discussion Novel Applications and Challenges for Plasma Mass Spectrometry, Luc Moens, Chairman Saturday, January 10, 1998 11. Plasma Source Emission/Mass Spectrometry: Solid Samples Sergio Caroli, Chairman 8 am (PL6) Plenary Lecture Willard W. Harrison, Glow Discharge as a Versatile Soilds Analysis Source for Atomic Spectrometry 9:15 (IL18) Invited Lecture R. Kenneth Marcus, Radio Frequency Glow Discharge: There’s More to Solids Analysis than the Determination of Transition Metals in Bulk Materials 9:45 (IL19) Invited Lecture Arne Bengtson, Advances in Glow Discharge Spectrometry: Challenges and Opportunities 12. Plasma Source Mass Spectrometry: Stable Isotope Applications R. Sam Houk, Chairman 2:00 (IL20) Invited Lecture Helen Crews, Stable Isotopes in Speciation and Human Bioavailability Studies 2:30 (IL21) Invited Lecture J.Sabine Becker and Hans-Joachim Dietze, Ultratrace and Precise Isotope Analysis by Double-Focusing Sector Field ICP-MS. 4:30 (PD5) Panel Discussion. New Spectrometers for Plasma Source Mass Spectrometry: Maturity versus Innovation, Norbert Jakubowski, Chair 5:30 Conference Closing 1998 Winter Conference Short Courses Friday, January 2 - Sunday, January 4, 1998 Summary Analysis SA-01 Trace Analysis of Biomedical Materials, January 3, 8 am, David E. Nixon SA-02 Analysis of Foods and Food Products, January 4, 7 pm, Gerald J. DeMenna SA-03 Analysis of Metals and Related Materials, January 2, 1 pm, SA-04 Analysis and Wet Chemical Processing of Semiconductor Materials, January 4, 8 am, Steven A. Koch and Karen P. Madden SA-05 Atomic Spectrochemical Analysis of Ceramics, January 3, 7 pm, José Broekaert SA-06 Geoanalysis I Atomic Emission Spectrometry, January 3, 1 pm, Isaac B. Brenner, Robert I. Botto SA-07 Geoanalysis II Mass Spectrometry, January 4, 8 am, Isaac B. Brenner, Howard Taylor, and Detlef Günther SA-08 Petroleum Analysis with Plasma Spectrometry, January 2, 7 pm, Robert I. Botto SA-09 Water Quality Applications and Environmental Chemistry, January 4, 7 pm, Howard Taylor SA-10 Spectroscopic Techniques and Applications in a Pharmaceutical Laboratory, January 3, 7 pm, Nancy Lewen, Martha Schenberger, and Mark D. Argentine Instrumentation SI-01 Calibration and Data Evaluation in Atomic Spectrometry, January 4, 8 am, José Broekaert SI-02 Method Validation and Measurement Uncertainty: Advanced Concepts in Analytical Quality Assurance, January 3, 8 am, Wolfhard Wegscheider SI-03 High Resolution ICP-MS, January 2, 7 pm, Luc Moens and Norbert Jakubowski SI-04 Glow Discharge Atomic Emission and Mass Spectrometry, January 4, 8 am, R. Kenneth Marcus SI-05 Time-of-Flight Mass Spectrometry for Elemental Analysis, January 4, 1 pm, Gary M. Hieftje SI-06 ICP-Mass Spectrometry I: Introduction, January 3, 8 am, R. Sam Houk SI-07 ICP-Mass Spectrometry II: Advanced Topics, January 3, 1 pm, R. Sam Houk SI-08 Fundamentals of Quadrupole Mass Spectrometry (How Do Quad Actually Work?), January 4, 8 am, Don Douglas SI-09 Inorganic Electrospray Mass Spectrometry, January 3, 1 pm, Gary Horlick SI-10 Software for When Things Go Wrong, January 3, 7 pm, Eric Salin and Christine Sartoros SI-11 Ion Trap Mass Spectrometry, January 4, 7 pm, David W. Koppenaal, Gregory C. Eiden, and Charles J. Barinaga SI-12 Microwave Plasma Spectrochemical Analysis, January 2, 7 pm, José Broekaert SI-13 Selecting an ICP-AES for Your Laboratory, January 3, 8 am, Robert I. Botto SI-14 Evaluation and Control of ICP Sequential Systems, January 3, 1 pm, Jean-Michel Mermet Sample Introduction SS-01 Sample Presentation in ICP Spectrometry, January 2, 7 pm, Cameron McLeod, Barry L. Sharp, and Dan Wiederin SS-02 Electrothermal Vaporization for Atomic and Mass Spectrometry, January 4, 1 pm, D. Conrad Grégoire SS-03 Flow Injection Analysis Techniques and Applications, January 4, 1 pm, Cameron McLeod and Julian F. Tyson SS-04 Vapor Generation Atomic and Mass Spectrometry, January 3, 1 pm, Les Ebdon and Steve J. Hill SS-05 Laser Ablation Atomic and Mass Spectrometry, January 2, 1 pm, Henry Longerich, and Detlef Günther SS-06 Nebulizer Characteristics, Design, and Routine Operation for ICP-AES and ICP/MS, January 3, 7 pm, Richard F. Browner SS-07 Plasma Spectroscopic Detection in Chromatography, January 4, 1 pm, Peter C. Uden and Joseph A. Caruso SS-08 Sample Introduction for ICP-AES and ICP/MS, January 2, 1 pm, Richard F. Browner SS-09 Slurry Nebulization Atomic and Mass Spectrometry, January 3, 8 am, Les Ebdon and Steve J. Hill SS-10 Solid Sample Introduction Techniques and Instrumentation, January 4, 1 pm, Nimal De Silva Techniques ST-01 Advanced Sample Preparation for Plasma Spectrometry, January 3, 8 am, Mark Tatro ST-02 Applications of Isotope Dilutions and Isotopic Measurements, January 4, 7 pm, Phillip Miller ST-03 Simulation of RF Analytical ICPs, January 4, 7 pm, Javad Mostaghimi and Pierre Proulx ST-04 Element Preconcentration in Trace Analysis, January 4, 1 pm, Günter Knapp and Rossario Pereiro ST-05 Oral Presentation Skills for Scientists, January 2, 1 pm, Steven A. Koch ST-06 Typical Errors in ICP Analyses and How to Avoid Them, January 2, 1 pm, Mark Tatro ST-07 Microwave Sample Preparation, January 3, 1 pm, H.M. "Skip" Kingston ST-08 Clean Microwave Digestions for Trace Element Analysis, January 4, 7 pm, H.M. "Skip" Kingston ST-09 Plasma Analysis Quality Control and Assessment Procedures, January 4, 8 am, Fred L. Fricke and Karen A. Wolnik ST-10 Plasma Diagnostics: Fundamentals, Measurements, Applications, January 3, 7 pm, John W. Olesik ST-11 Helium-Based Plasma Sources for Analytical Spectrometry, January 4, 1 pm, Akbar Montaser ST-12 Plasmas in Gases Other than Argon for Spectrochemical Analysis, January 4, 7 pm, Akbar Montaser ST-13 Mixed Gas Plasma for ICP-MS, January 2, 1 pm, Les C. Ebdon and Steve J. Hill ST-14 ICP-MS Interference Removal, January 3, 8 am, Les C. Ebdon and Hywel Evans ST-15 Simplex Optimization for Atomic and Mass Spectrometry, January 2, 7 pm, Les C. Ebdon and Steve J. Hill ST-16 Trace Element Speciation, January 3, 7 pm, Cameron McLeod, Olivier Donard, and Les Ebdon ST-17 Communicating Effectively Through Slide, Overhead, Computer Display and Multimedia Presentations, January 4, 8 am, Richard F. Browner ST-18 Preparing Your Laboratory for ICP-MS, January 3, 7 pm, Ela Bakowska ST-19 Contamination Issues in Trace Elemental Analysis, January 2, 7 pm, Paul Gaines and Mitch Dolobowsky Listed by Date and Time Friday, January 2, 1998, 1 pm - 5 pm SA-03 Analysis of Metals and Related Materials SS-05 Laser Ablation Atomic and Mass Spectrometry, Henry Longerich, and Detlef Günther SS-08 Sample Introduction for ICP-AES and ICP/MS, Richard F. Browner ST-05 Oral Presentation Skills for Scientists, Steven A. Koch ST-06 Typical Errors in ICP Analyses and How to Avoid Them, Mark Tatro ST-13 Mixed Gas Plasma for ICP-MS, Les C. Ebdon and Steve J. Hill Friday, January 2, 1998, 7 pm - 11 pm SA-08 Petroleum Analysis with Plasma Spectrometry, Robert I. Botto SI-03 High Resolution ICP-MS, Luc Moens and Norbert Jakubowski SI-12 Microwave Plasma Spectrochemical Analysis, José Broekaert SS-01 Sample Presentation in ICP Spectrometry, Cameron McLeod, Barry L. Sharp, and Dan Wiederin ST-15 Simplex Optimization for Atomic and Mass Spectrometry, Les C. Ebdon and Steve J. Hill ST-19 Contamination Issues in Trace Elemental Analysis, Paul Gaines and Mitch Dolobowsky Saturday, January 3, 1998, 8 am - 12 noon SA-01 Trace Analysis of Biomedical Materials, David E. Nixon SI-02 Method Validation and Measurement Uncertainty: Advanced Concepts in Analytical Quality Assurance, Wolfhard Wegscheider SI-06 ICP-Mass Spectrometry I: Introduction, R. Sam Houk SI-13 Selecting an ICP-AES for Your Laboratory, Robert I. Botto SS-09 Slurry Nebulization Atomic and Mass Spectrometry, Les Ebdon and Steve J. Hill ST-01 Advanced Sample Preparation for Plasma Spectrometry, Mark Tatro ST-14 ICP-MS Interference Removal, Les C. Ebdon and Hywel Evans Saturday, January 3, 1998, 1 pm - 5 pm SA-06 Geoanalysis I Atomic Emission Spectrometry, Isaac B. Brenner and Robert I. Botto SI-07 ICP-Mass Spectrometry II: Advanced Topics, R. Sam Houk SI-09 Inorganic Electrospray Mass Spectrometry, Gary Horlick SI-14 Evaluation and Control of ICP Sequential Systems, Jean-Michel Mermet SS-04 Vapor Generation Atomic and Mass Spectrometry, Les Ebdon and Steve J. Hill ST-07 Microwave Sample Preparation, H.M. "Skip" Kingston Saturday, January 3, 1998, 7 pm - 11 pm SA-05 Atomic Spectrochemical Analysis of Ceramics, José Broekaert SA-10 Spectroscopic Techniques and Applications in a Pharmaceutical Laboratory, Nancy Lewen, Martha Schenberger, and Mark D. Argentine SI-10 Software for When Things Go Wrong, Eric Salin and Christine Sartoros SS-06 Nebulizer Characteristics, Design, and Routine Operation for ICP-AES and ICP/MS, Richard F. Browner ST-10 Plasma Diagnostics: Fundamentals, Measurements, Applications, John W. Olesik ST-16 Trace Element Speciation, Cameron McLeod, Olivier Donard, and Les Ebdon ST-18 Preparing Your Laboratory for ICP-MS, Ela Bakowska Sunday, January 4, 1998, 8 am - 12 noon SA-04 Analysis and Wet Chemical Processing of Semiconductor Materials, Steven A. Koch and Karen P. Madden SA-07 Geoanalysis II Mass Spectrometry, Isaac B. Brenner, Howard Taylor, and Detlef Günther SI-01 Calibration and Data Evaluation in Atomic Spectrometry, José Broekaert SI-04 Glow Discharge Atomic Emission and Mass Spectrometry, R. Kenneth Marcus SI-08 Fundamentals of Quadrupole Mass Spectrometry (How Do Quad Actually Work?), Don Douglas ST-09 Plasma Analysis Quality Control and Assessment Procedures, Fred L. Fricke and Karen A. Wolnik ST-17 Communicating Effectively Through Slide, Overhead, Computer Display and Multimedia Presentations, Richard F. Browner Sunday, January 4, 1998, 1 pm - 5 pm SI-05 Time-of-Flight Mass Spectrometry for Elemental Analysis, Gary M. Hieftje SS-02 Electrothermal Vaporization for Atomic and Mass Spectrometry, D. Conrad Grégoire SS-03 Flow Injection Analysis Techniques and Applications, Cameron McLeod and Julian F. Tyson SS-07 Plasma Spectroscopic Detection in Chromatography, Peter C. Uden and Joseph A. Caruso SS-10 Solid Sample Introduction Techniques and Instrumentation, Nimal De Silva ST-04 Element Preconcentration in Trace Analysis, Günter Knapp and Rossario Pereiro ST-11 Helium-Based Plasma Sources for Analytical Spectrometry, Akbar Montaser Sunday, January 4, 1998, 7 pm - 11 pm SA-02 Analysis of Foods and Food Products, Gerald J. DeMenna SA-09 Water Quality Applications and Environmental Chemistry, Howard Taylor SI-11 Ion Trap Mass Spectrometry: Theory and Techniques for Elemental Applications, David W. Koppenaal, Gregory C. Eiden, and Charles J. Barinaga ST-02 Applications of Isotope Dilutions and Isotopic Measurements, Phillip Miller ST-03 Simulation of RF Analytical ICPs, Javad Mostaghimi and Pierre Proulx ST-08 Clean Microwave Digestions for Trace Element Analysis, H.M. "Skip" Kingston ST-12 Plasmas in Gases Other than Argon for Spectrochemical Analysis, Akbar Montaser Ramon M. Barnes, Chairman Winter Conference on Plasma Spectrochemistry Department of Chemistry Lederle Graduate Research Center University of Massachusetts Box 34510 Amherst, MA 01003-4510 Telephone (413) 545-2294 Facsimile (413) 545-3757 Internet winterconf@chem.umass.edu Internet ramon.m.barnes@chemistry.umass.edu ****************************************************************************** From: getarr@tiac.net (getarr) Date: Mon, 10 Mar 1997 09:51:09 -0500 (EST) Subject: MALDI-TOF Organization: * Whoever, Am I replying to the right place concerning the availability of DE-MALDI-TOF's? I am interested, but would have to scrounge for funds, and I need to know the manufacturer and a more complete description of the system in order to do that. Are there still instruments available and, if so, where are they located? George E. Tarr PProSeeq S. Hamilton, MA ****************************************************************************** From: Frank_Hollis-1@sbphrd.com.see-sig (Triple Quadrophenic) Date: 10 Mar 1997 14:56:27 GMT Subject: Re: Adduct of proteins at mass 96? Organization: SB In article <5fpate$ms7@acmey.gatech.edu>, heino.prinz@mpi-dortmund.mpg.de (Heino Prinz) dusted off the quill, prised open the inkwell and wrote... } }Hallo Everyone, } }Doing ESI-MS of proteins, I have often encountered an adduct of mass }96 to the expected molecular weight. These proteins have all been }expressed in E.Coli and the people involved say that TFA ( likely }candidate for such an adduct) had never been used at any stage with }these proteins. } }Does anybody have an idea regarding the nature of these adducts? Any }suggestions would be welcome } It's my experience that the TFA need not necessarily be in the sample you're examining. If you have something that has an affinity for TFA, and TFA has been anywhere near the system in the recent past, then you'll see the adducts. -- Frank J Hollis, Mass Spectroscopy, SmithKline Beecham, Welwyn, UK Frank_Hollis-1@sbphrd.com or fjh4@tutor.open.ac.uk http://www.geocities.com/CapeCanaveral/Lab/1550/ These opinions have not been passed by 3 committes, 7 subcommittees and continuous improvement teams. So they can't be the opinions of my employer ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 10 Mar 1997 17:48:24 GMT Subject: Re: Best MS config for peptides? Organization: AOL http://www.aol.com Some thoughts on your questions > Is it important to have extended mass range (2000 amu vs > standard 1000 amu)? YES, because many peptides are over 1,000 (most?) this is vital. An argument might be made for the multiple charging phenomena alieviating this requirement (In other words anything bigger than 1,000 would charge up at least twice and then allow one to still get MW by math) In pratice however you will find that you need both those measurements (especially in unresloved mixtures). Running in profile mode at good resolution can help in determining the charge state of unassigned peaks. Adding a chromatographic system can further help to resolve some ambiguities. It may be possible to do some useful mapping work (ie QC/synthesis confirmation) with only 1000 amu if that is what your budget allows. Some unknowns will more greatly tax your analytical skills with this limitation. > Is API essential? If so, is AP-ES or AP-CI better, and how much > worse is the worse of the two? APCI is not that useful for most cases encountered in peptide work. Other useful techniques include MALDI and FAB. In a lab without someone who takes an interest of some sort in MS (does such a place exist?) a laser device may be more robust and less frustrating. The ability to do on line LC-MS and fast MW confirmations is a real nice feature which would not be given up by those who use it. The ESI systems have a little steeper learning curve, although I know that like all things the laser systems have "tricks" as well. > How would particle beam and thermospray compare with API for > these purposes? CRAPPILY, Both these system apply too much energy to the sample. I have got spectra of peptides on both these interfaces but it is not the way I'd ever choose to do it. The PB interface sprays the peptide solution onto a a hot stainless surface in the presence of a beam of 70eV electrons. This means you are usually looking at thermal fragments. The TSP can just give molecular ions and multiple charging but it is over all a more cumbersome and destructive device. One other thing to consider might be the ability to add a new ultra low flow source. These allow the analyisis of sub-picomole quantities of peptides routinely. The ability to auto-mate the analysis may be important down the road as well. Your area may have a Mass Spec Users group that has monthly events. If so contact them and attend a few so that you might meet others in your situation. Matt Sweeney ****************************************************************************** From: atlantis@netcom.com (JJ Miranda) Date: Tue, 11 Mar 1997 05:52:31 GMT Subject: MALDI-TOF high mass Matrixes Organization: Netcom On-Line Services Hi all, I was wondering if anyone could please clear up a debate for me. In our lab, some results have shown that certain matrixes are more effective at giving a stronger signal for samples greater than about 10kDa or 15kDa. I normally use alpha-cyano, but I can't seem to pick up the signal of larger peptides in a mixture with smaller ones (which are easily detected). On a MALDI-TOF in linear mode, which matrixes are best to try with high mass peptides? Sincere regards, JJ Miranda Please reply by email. ****************************************************************************** From: msl <73530.1207@CompuServe.COM> Date: 11 Mar 1997 13:50:50 GMT Subject: Spares for VG/TIMS for sale Organization: CompuServe, Inc. (1-800-689-0736) We have various spares for the VG 354 series mass spec for sale. Parts include an ion source, filament shields, spacers and more. If you would like a detailed list e-mail us for more information. Regards Michael S. Leary O'Hara Spectrometer Service ****************************************************************************** From: Peter Ockenfels Date: Tue, 11 Mar 1997 22:53:59 +0100 Subject: Re: MS Chemstation data export II Organization: University of Freiburg Hi, there is a macro called "tabtic.mac" which has to reside in the msexe or macro directory. It converts the TIC to a excel spreadsheet. Drop me an e-mail an I attach it to a mail, It should run under W95-version of the Chemstation-software. Cheers Peter -- .... Dr. Peter Ockenfels Forstzoologisches Institut der Universitaet Freiburg Aussenstelle Wittental Fohrenbuehl 27 79252 STEGEN .... ****************************************************************************** From: "Dave White" Date: 12 Mar 1997 15:14:43 GMT Subject: Moving corporate office Organization: SpectraChrom Software Research Scientific Services, Inc., who have been headquartered in Raleigh, North Carolina since inception, are in the process of moving their corporate office to Washington DC. We are presently finalizing the lease on our new offices, and expect the move to occur towards the end of March, or early April. Our 1-800 number will remain the same, but our regular phone number will obviously change. As part of this move, we have a couple of VG 70E mass spectrometers which we are selling cheaply, rather than have to move them to DC. For more information, contact Bob Moffatt at RSS. Check out our web site at http://netnow.micron.net/~spectrac/rsshome.htm towards the end of this month for more information. ****************************************************************************** From: dfettero@ahoynet.com (Dean D. Fetterolf) Date: Wed, 12 Mar 1997 17:18:23 GMT Subject: CE/MS Interface ??? Organization: Monumental Network Systems We would like to inteface an HP 3DCE with a Finnigan LCQ and/or TSQ with an electrospray source. We have the CE/MS adapter kit from Finnigan. What size current limiting resistor should we use? Where does it go? Any helpful hints would be appreciated. Dean D. Fetterolf dfettero@mnsinc.com http://www.mnsinc.com/dfettero/home.html ****************************************************************************** From: dfettero@ahoynet.com (Dean D. Fetterolf) Date: Thu, 13 Mar 1997 13:10:35 GMT Subject: CE/MS Interface?? Organization: Monumental Network Systems We would like to inteface an HP 3DCE with a Finnigan LCQ and/or TSQ with an electrospray source. We have the CE/MS adapter kit from Finnigan. What size current limiting resistor should we use? Where does it go? Any helpful hints would be appreciated. Dean D. Fetterolf dfettero@mnsinc.com http://www.mnsinc.com/dfettero/home.html ****************************************************************************** From: Date: 13 Mar 1997 14:58:31 GMT Subject: FAB vs ES Organization: Sprynet News Service To analyze/characterize peptides, proteins, nucleotides, and/or for general pharmaceutical research, would FAB be as useful as ES as a MS source? How would they compare in general for such purposes? At the moment I can get an HP 5989 LC/MS system with FAB for much less than one with electrospray. ****************************************************************************** From: candymkr@aol.com (Candymkr) Date: 13 Mar 1997 18:56:06 GMT Subject: Job Opport. Organization: AOL http://www.aol.com I'm sorry if this is not the right place for this, but I'm looking for an Applications Chemist. Small Petroleum Testing Company. Central NJ. Knowledge of AA, GC, MSD etc. Please send Resume with Salary req. to: TPC 119 W Grove St Westfield, NJ 07090 Or E-mail Candymkr@Aol.com ****************************************************************************** From: Drew Gibson Date: Thu, 13 Mar 1997 06:37:10 +0000 Subject: Re: Mass spectrum Organization: The Mad House Distribution: world On Wed, 5 Mar 1997 at 13:23:04, Bill Cotham wrote in Mass spectrum }Gary Lavigne wrote: }} }} To Stefan Knecht }} The Mass Spectrum may be 2-Pentanone,3,4-epoxy- which is a ketone }} compound as you suggested. }} Synonym = Ethanone,1-(3-methyloxiranyl)- }} CAS# 17257-79-3 }} Gary Lavigne }} } }Gary, } }My library spectrum of the above compound lacks a molecular ion (100 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ }amu). The spectrum in question has a peak at 100. I think it is 2,4 }pentanedione. } Does this matter ? Isn't the only requirement for EI library spectra be that they are acquired at 70eV ? This says nothing about data thresholding, ion source cleanliness, ion source temperature, all of which can affect molecular ion intensity (or whether it is even recorded in the thresholding case). -- Cheers, Drew ;^) }}} Not the opinions of my employer but <<< }}} what the hell, I'm posting from home !!! <<< ****************************************************************************** From: "Graeme Roberston" Date: Fri, 14 Mar 1997 14:40:30 GMT Subject: K and M multipliers Organization: * Does anyone know of an alternative source for K and M multipliers for a Hewlett Packard 5989B MS engine. They are listed under HP part no 05971-80103 and K and M number 7534M. List price in the UK. is stlg750 / $1200 U.S. a not inconsiderable sum for a consumable item. Do K and M supply direct or to known after market suppliers. I have heard of Z- tek as a possible source but their price seems high also. I would also welcome any comments concerning multiplier life expectancy, particularly with particle beam interfaces. Thanks ****************************************************************************** Date: Wed, 05 Mar 1997 23:24:18 GMT Subject: Unknown Mass Spectrum Organization: Legal Medicine Institute Dear colleagues. Recently, performing screening analyses by GC/MS on seizured materials, I have ciclically found a substance that I'm not able to identify and is not present on the mass spectra libraries that I normally use (Wiley and NBS 75 k). Is there anyone who can help me? Moderator's Note: The spectrum can be viewed at . Thanks in advance for your time. Best wishes from Padua, Italy Dr. Luciano Tedeschi Centre of Behavioural and Forensic Toxicology ****************************************************************************** From: gt6801a@prism.gatech.edu (Zhenyu Shou) Date: 14 Mar 1997 16:10:18 -0500 Subject: Re: K and M multipliers Organization: Georgia Institute of Technology You can buy from sis (scientific instrument service 1-908-188-5550 or http://www.sisweb.com or sis@sisweb.com) for that 5989 multiplier-it is a Galileo Channeltron I guess. I don't know the price for the 5989 but the multiplier for our instrument (5988) is 760$. As for the life expectancy of the multiplier used in particle beam interface, it depends on your usage of the instrument of course. We changed our multiplier last July and it worked fine now so I will say at least one year. Also, whenever your multiplier gets old, your autotune or quicktune will crank it up higher and higher each time you tune it. And when one day after you tune it, the a.tune file has a EMV of 3200 then you know it is the time to change it, or at least, buy one for back-up purpose. The interface doesn't seem to exert a very pronounced effect on the multiplier, at least not directly as far as I know. ****************************************************************************** From: mrheadmstr@aol.com Date: 15 Mar 1997 05:57:04 GMT Subject: Re: K and M multipliers Organization: AOL http://www.aol.com In article <5gbseh$1eo@acmex.gatech.edu>, "Graeme Roberston" writes: }Does anyone know of an alternative source for K and M multipliers for }a Hewlett Packard 5989B MS engine. They are listed under HP part no }05971-80103 and K and M number 7534M. List price in the UK. is }stlg750 / $1200 U.S. a not inconsiderable sum for a consumable item. Do }K and M supply direct or to known after market suppliers. I have }heard of Z- tek as a possible source but their price seems high also. } I would also welcome any comments concerning multiplier life }expectancy, particularly with particle beam interfaces. x-No Archive=Yes Not only does K&M sell directly to customers, you can also have your old multiplier rebuilt to original specs. Try contacting SpectraSource (I believe you can find them on the web) about their rebuild service. MrH ****************************************************************************** From: pjacobs@xs4all.nl (Peter Jacobs) Date: Sat, 15 Mar 1997 21:52:24 +0100 Subject: Dutch Society for Mass Spectrometry Organization: * Hi all, The new home page of the Dutch Society for Mass Spectrometry (NVMS - Nederlandse Vereniging voor Massaspectrometrie) can now be found at: http://www.xs4all.nl/~pjacobs/nvms.html This page gives information regarding the bi-annual meetings of the NVMS. Furthermore an overview of links to home pages of members of the Society as well as other relevant links world-wide can be found. Last but not least a diary with international mass spec meetings is presented. Please e-mail any comments or suggestions for improvement to: pjacobs@xs4all.nl Kind Regards, Peter Jacobs Dutch Society for Mass Spectrometry ****************************************************************************** From: Drew Gibson Date: Sun, 16 Mar 1997 06:48:16 +0000 Subject: Re: K and M multipliers Organization: The Mad House Distribution: world On Fri, 14 Mar 1997 at 10:56:01, Graeme Roberston wrote in K and M multipliers }Does anyone know of an alternative source for K and M multipliers for }a Hewlett Packard 5989B MS engine. They are listed under HP part no }05971-80103 and K and M number 7534M. List price in the UK. is }stlg750 / $1200 U.S. a not inconsiderable sum for a consumable item. Do }K and M supply direct or to known after market suppliers. I have }heard of Z- tek as a possible source but their price seems high also. } I would also welcome any comments concerning multiplier life }expectancy, particularly with particle beam interfaces. }Thanks I don't know about direct suppliers of these multipliers, just wanted to comment on the price. I used to use a 5989 (2.5 yrs ago), and changed from the channeltron multiplier to the K+M. The advantage of the K+M is that at the end of its 'life', all you need to replace is the front end 'phosphor', as its a photomultiplier IIRC. Thus 'replacements' are MUCH cheaper than a new multiplier, which is probably why the initial cost is much greater (you'll only ever need one K+M detector, just replace the little front end). IIRC they last longer than a channeltron too ? I used to use 'mine' with thermospray, which is considerably dirtier than PB (which I also used occasionally). Better than a channeltron for what I was doing, as far as life expectancy was concerned. -- Cheers, Drew ;^) }}} drew@thegibsons.demon.co.uk <<< ****************************************************************************** From: Administrator@mcphar01.med.nyu.edu (Administrator) Date: Sun, 16 Mar 1997 18:08:14 +0000 (GMT) Subject: Re: Adduct of proteins at mass 96? Organization: New York University In article <5fpate$ms7@acmey.gatech.edu>, heino.prinz@mpi-dortmund.mpg.de (Heino Prinz) says: } }Hallo Everyone, } }Doing ESI-MS of proteins, I have often encountered an adduct of mass }96 to the expected molecular weight. These proteins have all been }expressed in E.Coli and the people involved say that TFA ( likely }candidate for such an adduct) had never been used at any stage with }these proteins. } }Does anybody have an idea regarding the nature of these adducts? Any }suggestions would be welcome } }Yours sincerely, Heino Prinz } } } }Tel.:(+49)-231-1206-332 Fax:(+49)-231-1206-389 } } An article (J. Am. Soc. Mass Spectrom. (1990) 1,382) dealt with this problem in detail. The adduct is caused by the non-covalent attachment of sulfate or phosphate ions to your protein. Some proteins have a very strong interaction with these ions while other do not. Adding a soluble barium salt (such as barium acetate) to the protein solution will precipitate the sulfate or phosphate ions and remove the artifact from your spectra. TFA has nothing to do with it. Ron Beavis ****************************************************************************** From: "PC's a la Carte" Date: 17 Mar 1997 01:39:01 GMT Subject: For Sale: DG10 Incos Data System Organization: PC's a la Carte Used DG10 Incos Data System with Keyboard and Monitor Good for Spares $750 + Shipping -- PC's a la Carte 680 Northland Boulevard Suite 11 Cincinnati OH 45240, USA Phone: 1-800-989-0144 / (513) 825-0144 Fax: (513) 825-0163 E-Mail: sales@pcsalacarte.com Web: http://www.pcsalacarte.com ****************************************************************************** From: "PC's a la Carte" Date: 17 Mar 1997 01:41:05 GMT Subject: For Sale: Use SuperIncos MS Data System Organization: PC's a la Carte Nova 4X 16 slot chassis with CPU, Memory, MS10 and Disk/Tape Controller Priam 70MB Drive and Tape Streamer Monochrome Hires Terminal Good for spares $1, 000 + Shipping. -- PC's a la Carte 680 Northland Boulevard Suite 11 Cincinnati OH 45240, USA Phone: 1-800-989-0144 / (513) 825-0144 Fax: (513) 825-0163 E-Mail: sales@pcsalacarte.com Web: http://www.pcsalacarte.com ****************************************************************************** From: "Michael A. Roberts" Date: Mon, 17 Mar 1997 01:26:29 -0500 (EST) Subject: Re: Unknown Mass Spectrum Organization: * } }Dear colleagues. } }Recently, performing screening analyses by GC/MS on seizured }materials, I have ciclically found a substance that I'm }not able to identify and is not present on the mass spectra libraries }that I normally use (Wiley and NBS 75 k). Is there }anyone who can help me? } } Moderator's Note: } The spectrum can be viewed at } . } }Thanks in advance for your time. } }Best wishes from Padua, Italy }Dr. Luciano Tedeschi }Centre of Behavioural and Forensic Toxicology I was fortunate enough to be able to download and view the mass spectrum that you posted at the Georgia Tech FTP site, named "maietti.jpg". I think that I have a valid interpretation for you. The mass spectrum is consistent with a tetramer of phenyl rings: C6H5-C6H4-C6H4-C6H5 Notice that four of the five most abundant peaks are at m/z 77, 154, 229, and 308. I observe a prominent peak corresponding to a loss of the elements of C2H2 from the 77 peak (at m/z 51) as well as the 154 peak (at m/z 128), typical of a benzene ring. Although I had to eyeball it, it appears that the peak at m/z 309, relative to the peak at m/z 308, is consistent with a compound with about 20 carbons. I note that the compound is very stable to fragmentation until you can knock off a ring; thereafter, fragmentation begins to predominate. There is almost no fragmentation between m/z 308 and m/z 229; between 229 and 154, only one mass peak is interesting (m/z 183); and lower than 154, the fragmentation pattern begins to look like the EI of a biphenyl. May I suggest a high resolution spectrum to confirm these suspicions, if you have access to a suitable instrument? And whatever the compound really is, I would appreciate it if you would keep me informed of your progress in identifying it. And if you have any other questions, please feel free to contact me at the email address shown in my .signature file below. I hope this helps, and look forward to hearing from you. -- Michael Roberts | "Don't ever wrestle a pig. You'll both roberts@ucunix.san.uc.edu | get dirty and the pig will enjoy it." | -Attributed to many sources Speaking only for myself. | ****************************************************************************** From: Robert Forman Date: 17 Mar 1997 10:46:37 GMT Subject: Micro pH electrode measures pH of SMALLEST SAMPLES Organization: NetMedia A micro combination pH electrode is now available which can measure samples as small as 20 microliters which is a fraction of a single drop of liquid. The electrode fits into 96 well plates, microcentrifuge tubes, serum cups, capillary tubes, and NMR tubes. The probe is all solid state with a Teflon body making itvirtually unbreakable. It has a 1mm tip and 2 mm body and can be used with any standard pH meter. For further details contact Lazar Research Labs. Inc. at service@lazarlab.com or see the Lazar web site at http://www.lazarlab.com or fax 1-213-931-1434. ****************************************************************************** From: ligon@macgw1.crd.ge.com Date: Mon, 17 Mar 1997 13:55:28 -0300 Subject: Re: Unknown Mass Spectrum Organization: GE Corp R&D Center, Schenectady NY Unless my calculator is broken, I find that the tetramer shown below by Michael Roberts has a mass of 306. The mass shown on the "unknown mass spectrum" is 308. The general comments would seem to be reasonable but alas I am afraid the structure proposed is wrong. Also this proposed phenyl tetramer does appear in the NIST library. Woody In article <5gjfst$jvi@acmey.gatech.edu>, "Michael A. Roberts" wrote: } } I was fortunate enough to be able to download and view the mass spectrum } that you posted at the Georgia Tech FTP site, named "maietti.jpg". I } think that I have a valid interpretation for you. } } The mass spectrum is consistent with a tetramer of phenyl rings: } } C6H5-C6H4-C6H4-C6H5 } ****************************************************************************** From: shuster@socrates.berkeley.edu (David Shuster) Subject: HP 6890 GC/MS Date: Sat, 15 Mar 1997 02:47:11 GMT Organization: University of California, Berkeley I have an HP 6890 GC/MS with a 0.32 mm fused silica capillary column and a gas sampling valve set up such that I can expand a gas sample from a vacuum line into a sample loop to inject onto the GC. I have control over the volume and pressure of the sample. I am interested in the detection limits of this instrument, specifically the quadropole mass spec. Does anyone out there have any experience with the HP 6890 GC/MS? Does anyone have experience with a gas sampling valve? Should I be able to measure ppm CO2 or atmospheric CH4 concentrations with reproducible results? Suggestions are welcome Thank you for any input, David ****************************************************************************** From: Jon.Caners@nr1.ottawa.istar.net Date: 17 Mar 1997 23:58:21 GMT Subject: Weird stuff! Please help me out! Organization: iSTAR internet Incorporated Hello, I am doing a science fair project on mass spectrometry. I was wondering if somebody out there could help me out with something. In a mass spectrometer I understand a heavier ion is deflected less by a magnetic field than a lighter ion. What I did, was make a ramp and at the end attached a strong magnet. I then rolled ball-bearings (which were vouched for as being the same metal) and it seems that the heavier the ball-bearing, the more the magnet pulls it over. Personally I am at a loss as to why this is. I am hopeful that you would possibly be able to explain to me why this is occuring, or refer me to someone who would know the answer. Thanks for reading this. Jon Caners jon@cybertap.com ****************************************************************************** From: Colin Cook Date: Tue, 18 Mar 1997 13:21:04 +1100 Subject: Internal standards Organization: * I am looking for a supplier of internal standards for use in a mass spec screening procedure for pesticide residue analysis. I am after deuterated or 13C pesticides. An organochlorine or organophosphate would be best, but others are OK. I cannot find any supplier in my extensive collection of catalogs. Colin Cook Section Leader Dept Natural Resources & Environment State Chemistry Laboratory Melbourne, Australia Ph (+61 3) 9742 8753 ****************************************************************************** From: rxw13@cwru.edu (Dr. Ron) Date: Tue, 18 Mar 1997 09:58:36 E.S.T. Subject: Re: Weird stuff! Please help me out! Organization: Case Western Reserve University, Cleveland OH (USA) } Jon.Caners@nr1.ottawa.istar.net writes: }Hello, } I am doing a science fair project on mass spectrometry. I was }wondering if somebody out there could help me out with something. } In a mass spectrometer I understand a heavier ion is deflected }less by a magnetic field than a lighter ion. What I did, was make a }ramp and at the end attached a strong magnet. I then rolled }ball-bearings (which were vouched for as being the same metal) and it }seems that the heavier the ball-bearing, the more the }magnet pulls it over. } Personally I am at a loss as to why this is. I am hopeful }that you would possibly be able to explain to me why this is occuring, }or refer me to someone who would know the answer. Seems to me a couple of possibilities: In Mass Spec, the charge is constant for all relevant ions, thus deflection is inversely proportional to mass. I don't know if thats the case with your ball bearings, the greater the mass, the greater the induced magnetism - thus the "charge" would increase as the mass. Then theres the question of rolling resistence - not relevant in the vacuum of a real MS. I'd suggest trying the little hollow plastic-sphere magnets available in toy stores. Try filling them with different substances (nothing, water, sand, lead filings). Your analogy might work better when the size of the sphere is constant and the magnetic material is constant and ONLY the mass changes. ****************************************************************************** From: gs_klinger@pnl.gov (George S Klinger) Date: Tue, 18 Mar 1997 18:54:57 +0000 (GMT) Subject: Re: Weird stuff! Please help me out! Organization: Battelle Pacific Northwest National Labs (PN2L) Acceleration due to gravity will give each different sized ball the same velocity, but the kenetic energy will be different. So in the eq. eV = 1/2 mv^2 each ion formed in the mass spectrometer's ion source will have the SAME kinetic engery. Therefore ions with different mass to charge ratios will have DIFFERENT velocities; not the same velocity as your "ball down the ramp" model. Good try though, but you need a different model! Maybe try dropping them thru a viscus fluid, where the surface area to mass ratio will play a role. That might make the samller balls move more slowlly than the more massive ones, and your magnet might just do the trick. George In article <5gm5gc$iii@acmey.gatech.edu>, Jon.Caners@nr1.ottawa.istar.net says... } }Hello, } I am doing a science fair project on mass spectrometry. I was }wondering if somebody out there could help me out with something. } } In a mass spectrometer I understand a heavier ion is deflected }less by a magnetic field than a lighter ion. What I did, was make a }ramp and at the end attached a strong magnet. I then rolled }ball-bearings (which were vouched for as being the same metal) and it }seems that the heavier the ball-bearing, the more the }magnet pulls it over. } } Personally I am at a loss as to why this is. I am hopeful }that you would possibly be able to explain to me why this is occuring, }or refer me to someone who would know the answer. } } Thanks for reading this. } } Jon Caners } jon@cybertap.com } } ****************************************************************************** From: Guenter Eigendorf Date: Tue, 18 Mar 1997 14:57:13 -0800 Subject: peptide software Organization: * I am looking for PC based software to calculate amino acid compositions (natural and modified) for individual mass peaks in a peptide spectrum. Does anybody know of a source. Guenter Eigendorf Tel: 604 822-3235 Fax: 604 822-2847 e-mail: eigen@chem.ubc.ca ****************************************************************************** From: Drew Gibson Date: Tue, 18 Mar 1997 23:53:44 +0000 Subject: Re: Unknown Mass Spectrum Organization: The Mad House Distribution: world On Fri, 14 Mar 1997 at 10:56:30, Luciano wrote in Unknown Mass Spectrum }Dear colleagues. } }Recently, performing screening analyses by GC/MS on seizured }materials, I have ciclically found a substance that I'm }not able to identify and is not present on the mass spectra libraries }that I normally use (Wiley and NBS 75 k). Is there }anyone who can help me? } } } Moderator's Note: } The spectrum can be viewed at } . } } } }Thanks in advance for your time. } }Best wishes from Padua, Italy }Dr. Luciano Tedeschi }Centre of Behavioural and Forensic Toxicology } An interesting spectrum, with lots of multiples of 77. Are you *sure* that the molecular ion is 308 ? Some CI data would be useful here if available. What was the scan range ? Some interesting losses occur from m/z 308 and from m/z 229 - these peaks are small and may be background - could you confirm that they are still present after background subtraction, if this was not performed ? If at all possible (I realise it may not be), some information regarding the origin of the sample would be useful. Best wishes to you too, Dr. Tedeschi ! -- Cheers, Drew ;^) }}} drew@thegibsons.demon.co.uk <<< ****************************************************************************** From: Milan_Ralitsch@envirotest.com (Dr. Milan Ralitsch) Date: Wed, 19 Mar 1997 12:14:22 GMT Subject: Re: Internal standards Organization: Enviro-Test Laboratories On 18 Mar 1997 08:32:02 -0500, Colin Cook wrote: }I am looking for a supplier of internal standards for use in a mass }spec screening procedure for pesticide residue analysis. I am after }deuterated or 13C pesticides. An organochlorine or organophosphate }would be best, but others are OK. I cannot find any supplier in my }extensive collection of catalogs. You may be able to find what you are looking for at Cambridge Isotope Laboratories. I don't have their catalog in front of me right now, but they supply us with 13C- and 2H-labelled standards on a regular basis. You can request their catalogue from their web site at: http://www.isotope.com/ Hope this helps... ****************************************************************************** From: Martin@nezumi.demon.co.uk (Martin Tom Brown) Date: Tue, 18 Mar 97 21:12:15 GMT Subject: Re: Weird stuff! Please help me out! Organization: Nezumi In article <5gm5gc$iii@acmey.gatech.edu> Jon.Caners@nr1.ottawa.istar.net writes: } I am doing a science fair project on mass spectrometry. I was } wondering if somebody out there could help me out with something. } } In a mass spectrometer I understand a heavier ion is deflected } less by a magnetic field than a lighter ion. What I did, was make a } ramp and at the end attached a strong magnet. I then rolled } ball-bearings (which were vouched for as being the same metal) and it } seems that the heavier the ball-bearing, the more the } magnet pulls it over. Probably in part because the larger ball bearings get closer to the magnetic pole, and your magnetic field is non uniform. Also the bigger magnetic body will feel additional force, so it's not the same as a charged ion in a magnetic field. } Personally I am at a loss as to why this is. I am hopeful } that you would possibly be able to explain to me why this is occuring, } or refer me to someone who would know the answer. You might get something behaving in qualitatively the right sort of way if you put the magnet under the board so that the ball bearings are all essentially the same distance from the magnet as they pass. As long as you can get some mass dispersion you will have a demo. Maybe it's easier to cheat and use the difference in rolling resistance of the balls on a sloping plane to provide your mass discrimination. Inject them at constant speed facing slightly uphill. In real mass spectrometers people go to a lot of trouble to obtain a highly uniform magnetic field and minimise fringe fields at the start and end of the magnetic poles. It isn't too surprising you are finding it tricky to get a macroscopic system to work. Good luck! -- Martin Brown __ CIS: 71651,470 Scientific Software Consultancy /^,,)__/ ****************************************************************************** From: YONG HUANG Date: Wed, 19 Mar 1997 13:57:26 -0600 (CST) Subject: Magnet puzzle Organization: * Jon asked why bigger ball bearings deflect more than smaller ones in magnetic field. If I understand right, Jon does not put charge on the balls so that the force acted on ferromagnetic metal balls is completely different from Lorentzian force which is the force acted on a charge i.e. f=qvB (q=charge, v=velocity and B=magnetic field) while f=ma (m=mass, and a=acceleration and equals to v^2/r, r being the radius of curving motion). So given the same f as a first approximation, the lower m, the higher a and lower r. Since the v is actually different for different m, f is even smaller for larger ions after they come out of the electric acceleration field. This makes it more correct to say that the lower m, the lower r. If you demonstrate this on a "eye-observable" scale, don't forget to use charged balls. But I don't know how dry the ambient air should be in order to keep the charge on them for a sufficiently long time. BTW, any physicist out there knows the formular of the force for ferromagnetic attraction of a magnet to iron (not ion, it's neutral)? Yong ****************************************************************************** From: James Little Date: Wed, 19 Mar 1997 15:47:05 -0500 Subject: Answer to Question Organization: * Someone had proposed a tetramer of benzene, but that has a molecular weight of 306 instead of 308. Another possibility would be a tetramer formed from two moles of pyridine and 2 moles of benzene. }Dear colleagues. } }Recently, performing screening analyses by GC/MS on seizured }materials, I have ciclically found a substance that I'm }not able to identify and is not present on the mass spectra libraries }that I normally use (Wiley and NBS 75 k). Is there }anyone who can help me? } } Moderator's Note: } The spectrum can be viewed at } . } }Thanks in advance for your time. } }Best wishes from Padua, Italy }Dr. Luciano Tedeschi }Centre of Behavioural and Forensic Toxicology James Little Eastman Chemical Company Tel. No. 423-229-8685 ****************************************************************************** From: Michael Chester Date: Thu, 20 Mar 1997 04:30:35 -0500 Subject: Re: K&M Multipliers Organization: * }"Graeme Roberston" wrote: }Does anyone know of an alternative source for K and M multipliers for }a Hewlett Packard 5989B MS engine. They are listed under HP part no }05971-80103 and K and M number 7534M. List price in the UK. is }stlg750 / $1200 U.S. a not inconsiderable sum for a consumable item. Do }K and M supply direct or to known after market suppliers. I have }heard of Z- tek as a possible source but their price seems high also. } I would also welcome any comments concerning multiplier life }expectancy, particularly with particle beam interfaces. }Thanks GSG Analytical Instruments Ltd. can supply you with just about any multiplier you want, whether it be K&M, Galileo, DeTech etc.etc. The phone number for GSG is 01925 418 044. Regards Mike ****************************************************************************** From: ron@ftc.gov.pe.ca (Ron Skinner) Date: Thu, 20 Mar 1997 07:56:10 LOCAL Subject: Re: Internal standards Organization: PEI Food Technology Centre }I am looking for a supplier of internal standards for use in a mass }spec screening procedure for pesticide residue analysis. I am after }deuterated or 13C pesticides. An organochlorine or organophosphate }would be best, but others are OK. I cannot find any supplier in my }extensive collection of catalogs. }Colin Cook }Section Leader }Dept Natural Resources & Environment }State Chemistry Laboratory }Melbourne, Australia }Ph (+61 3) 9742 8753 Have you tried Supelco a now a division of Sigma Aldrich Sigma-Aldrich Pty. Ltd. Unit 2, 14 Anella Ave. Castle Hill NSW 2154 tel: 02 9841 0555 1-800-800 097 fax 1 800 800 096 02 9841 0500 They supply deuterated standards of a number of compounds. Ron ****************************************************************************** From: Stefan Knecht Date: Thu, 20 Mar 1997 13:31:22 +0100 Subject: home page for chemists Organization: Heinrich Heine Universitaet Duesseldorf The Homepage for Chemists This home page contains over 700 links about chemistry. All links are listed by subject such as software, databases, newsgroups, chemical industry, periodic tables, jobs etc. A short abstract follows every link. Have a look at it!!!! http://www-public.rz.uni-duesseldorf.de/~knecht/englisch/chemeng.htm If you are webmaster of a chemical home page kindly add a link to this home page on your page. For this purpose, have a look at the sponsors' page at http://www-public.rz.uni-duesseldorf.de/~knecht/englisch/sponsors/bannereng.htm ****************************************************************************** From: sjm@kore.co.uk (Steve Mullock) Date: Thu, 20 Mar 1997 10:36:04 GMT Subject: Re: K and M multipliers Organization: Kore Technology Ltd. "Graeme Roberston" wrote: }Does anyone know of an alternative source for K and M multipliers for }a Hewlett Packard 5989B MS engine. They are listed under HP part no }05971-80103 and K and M number 7534M. List price in the UK. is }stlg750 / $1200 U.S. a not inconsiderable sum for a consumable item. } snip < Galileo do an equivalent multiplier, model number 5778 part number 1343-0500. As you are in the UK we are the appropriate distributer. The price would be significantly less than £750, please contact us for a proper quote if desired. If you consult our web pages you will find guidance on identifying the correct Galileo multiplier for both this instrument and a wide range of others from various manufacturers. Hope this is of use -- Dr S.J. Mullock Kore Technology Ltd. Tel: 44 (0) 1223 420840 Fax: 44 (0) 1223 426041 http://www.kore.co.uk/users/ab48/ ****************************************************************************** From: sjm@kore.co.uk (Steve Mullock) Date: Thu, 20 Mar 1997 10:36:05 GMT Subject: Re: Weird stuff! Please help me out! Organization: Kore Technology Ltd. gs_klinger@pnl.gov (George S Klinger) wrote: }Acceleration due to gravity will give each different sized ball the same }velocity, but the kenetic energy will be different. So in the eq. }eV = 1/2 mv^2 }each ion formed in the mass spectrometer's ion source will have the SAME }kinetic engery. Therefore ions with different mass to charge ratios will }have DIFFERENT velocities; not the same velocity as your "ball down the }ramp" model. Good try though, but you need a different model! }snip< How about launching them with a sprung plunger, as pinball machines do. This might at least start them with roughly the same energy. To simulate the action of a magnetic field on a charged particle is going to be more tricky. Even if you put the same sized amount of steel in each ball and adjust the weight another way it will be wrong as in a magnetic mass spec. the force is velocity dependent. You could try making the balls conducting (a shell with fillings of different weights) but NOT ferromagnetic. With a powerful enough magnet the induced current might give you a deflection and if so the lighter ones would deflect further. Even better, give up on the old 'magnetic' technology and simulate a time-of-flight. These are newer and much more exiting (I'm not really biased because we design and build them :-) For some real ones , see our web page. -- Dr S.J. Mullock Kore Technology Ltd. Tel: 44 (0) 1223 420840 Fax: 44 (0) 1223 426041 http://www.kore.co.uk/users/ab48/ ****************************************************************************** From: dfettero@ahoynet.com (Dean D. Fetterolf) Date: Thu, 20 Mar 1997 12:47:52 GMT Subject: Counterterrorism R&D BAA - cbd.txt Organization: Monumental Network Systems COUNTER-TERRORISM/ANTI-TERRORISM BROAD AGENCY ANNOUNCEMENT Category: A--Research and Development (PROCUREMENTS) Date Published: March 17, 1997 Dean D. Fetterolf dfettero@mnsinc.com http://www.mnsinc.com/dfettero/home.html ------------------------------------------------------------------------ Contact: DIRECTORATE OF CONTRACTING, ATTN: STEAP-PR-C, BLDG 4118 SUSQUEHANNA BLVD, APG MD 21005-5001 POC Contract Specialist Shonn Moore 410 278-0879 (Site Code DAAD05) Synopsis: COUNTER-TERRORISM/ANTI-TERRORISM BROAD AGENCY ANNOUNCEMENT SOL DAAD05-97-T-1342 DUE 050597 The Directorate of Contracting (DoC), Aberdeen Proving Ground (APG), is soliciting concepts for innovative research and development projects. The following mission areas included for these projects are: Assault Support--This area is for equipment and systems to support specialized force offensive operations; Explosives Detection and Explosive Ordnance Disposal This area is for equipment and systems to detect and disable improvised explosive devices (IED), this area does not include conventional military ordnance in battlefield scenarios nor does it include minefield or range clearance of any kind; Weapons of Mass Destruction Countermeasures--This area is for equipment and systems to detect, assess, mitigate, render- safe, and decontaminate improvised nuclear, chemical, and biological devices in non-battlefield scenarios; Physical Security and Infrastructure Protection--Equipment and systems to protect key national assets, both government and private, domestically and internationally; and Investigative Support and Forensics--Equipment and systems to aid in the development of evidence to assist in gathering evidence for the prosecution of terrorists. DoC is the procuring office for the Office of Special Technology, located in Fort Washington, MD, who is the program office sponsoring the above mission areas. The information in this announcement, in conjunction with the information in the BAA package (that contractors will need to request), constitutes a solicitation of concepts from those offerors capable of satisfying the government's needs. No other information will be provided. The government encourages non-profit organizations, educational institutions, small businesses, small disadvantaged businesses, government laboratories, and HBCU/MIs to submit research concepts for consideration. The BAA selection process will be conducted in three phases. The Phase I submission shall be a one page vugraph chart and/or five minute maximum length videotape. The Phase I submittal will be due no later than 30 days from the issue date of the BAA package. Phase I concepts received after that date will not be considered by the government. Phase II will include those offerors selected for continued evaluation by the government, who will need to submit a ten page maximum white paper, further describing the selected mission area with the proposed technical solution. Phase III, the final phase, will be a full proposal concept, requested only from those offerors with a high probability of receiving a contract. The length of the full proposals shall be limited to 50 pages. Potential offerors are encouraged to request the BAA package from DoC as soon as possible to ensure compliance with the vugraph submittal deadline. Potential offerors must obtain the BAA package itself, DAAD05-97-T-1342, which describes the process for concept submission/evaluation. This BAA is available upon written or facsimile request to Ms. Barbara Shirley. The request shall include company name, mailing address, and BAA number, DAAD05-97-T-1342. The facsimile telephone number is 410-278-0900. Status of BAA package requests may be made by calling 410-278-0842, Ms. Barbara Shirley. Questions of a contractual nature may be directed to Mr. Shonn Moore, 410-278-0879 or Mr. David Hart, (I-072 SN042188) ****************************************************************************** From: hines@cgl.ucsf.EDU (Wade Hines) Date: 20 Mar 97 14:31:52 GMT Subject: Re: Weird stuff! Please help me out! Organization: UCSF Computer Graphics Lab Martin@nezumi.demon.co.uk (Martin Tom Brown) writes: }In article <5gm5gc$iii@acmey.gatech.edu> Jon.Caners@nr1.ottawa.istar.net }writes: }} I am doing a science fair project on mass spectrometry. I was }} wondering if somebody out there could help me out with something. }} }} In a mass spectrometer I understand a heavier ion is deflected }} less by a magnetic field than a lighter ion. What I did, was make a }} ramp and at the end attached a strong magnet. I then rolled }} ball-bearings (which were vouched for as being the same metal) and it }} seems that the heavier the ball-bearing, the more the }} magnet pulls it over. }Probably in part because the larger ball bearings get closer }to the magnetic pole, and your magnetic field is non uniform. }Also the bigger magnetic body will feel additional force, }so it's not the same as a charged ion in a magnetic field. } }} Personally I am at a loss as to why this is. I am hopeful }} that you would possibly be able to explain to me why this is occuring, }} or refer me to someone who would know the answer. }You might get something behaving in qualitatively the right sort of }way if you put the magnet under the board so that the ball bearings }are all essentially the same distance from the magnet as they pass. }As long as you can get some mass dispersion you will have a demo. }Maybe it's easier to cheat and use the difference in rolling resistance }of the balls on a sloping plane to provide your mass discrimination. }Inject them at constant speed facing slightly uphill. If the project is just about mass spectrometry and not specifically about magnetic sector mass spectrometers, time of flight can be a nice demonstation. E=1/2mv^2 is a rather simple equation and there are some rather nice results in modern time of flight mass spectrometers that ought to be changing their reputation as a poor second cousin to magnetic sector instruments, but I will admit to a new found bias toward time of flight. I won't offer how to give a big ball and a little ball the same Energy. That's the thing to learn which is waht I hope a science project is all about. Cheers, Wade Hines }In real mass spectrometers people go to a lot of trouble to obtain }a highly uniform magnetic field and minimise fringe fields at the }start and end of the magnetic poles. It isn't too surprising you }are finding it tricky to get a macroscopic system to work. ****************************************************************************** From: Shai RUBNOV Date: Thu, 20 Mar 1997 21:37:36 +0200 Subject: ms of a new natural product. Organization: The hebrew University of Jerusalem Hello every one. I have a new natural product isolated from a n israeli plant. It has a very strong pick at 84 m/z. It think it might be a piperidine alkaloids. Does any one knows something about ms of such natural products? Thanks in advance. Shai Rubnov -- ///\\\\\ / _ ^ \ ( (o) (o) ) *=====OOOo=====(_)======OOOo=====* | Shai RUBNOV | | Golomb 47/4 Herzeliya | | ISRAEL | | E-mail : srub@cc.huji.ac.il | | cellcom : 052446498 | *=========.Oooo==================* ( ) oooO. \ ( ( ) \_) ) / (_/ ****************************************************************************** From: li@pine.circa.ufl.edu Date: 21 Mar 1997 02:00:38 GMT Subject: Ph.D. analytical chemist/spectroscopist position? Organization: University of Florida - CIRCA Hi, I am a Ph.D. analytical chemist/spectroscopist working at a national laboratory, and I am looking for an industrial position. Your help is greatly appreciated. My technical skills include spectroscopy (IR,Raman,MS,UPS,UV-VIS-NIR), thermal analysis (TGA,TGA-FTIR,DSC), and chromatography (GC,GC-MS, GC-FTIR,HPLC,LC-MS). Please contact me at s_li@pnl.gov if you have any information. Thanks a lot. ****************************************************************************** From: rmerkle@uga.cc.uga.edu (Roberta K. Merkle) Date: 21 Mar 1997 16:52:27 GMT Subject: CARBOHYDRATE COURSES Organization: CCRC, Univ. Georgia Techniques for Characterization of Complex Carbohydrates Four courses will be offered in 1997 at the Complex Carbohydrate Research Center (CCRC) of the University of Georgia: 1. Separation and Characterization of Glycoprotein Oligosaccharides (June 9-13), 2. Structural Analysis of Oligosaccharides (June 16-20), 3. Mass Spectrometry and MS/MS Analysis of Glycoconjugates (June 23-27), 4 NMR of Carbohydrates (July 14-18). Courses will consist of hands-on laboratory work, demonstrations and lectures; lab manual including selected analytical techniques and references will be provided. For further information contact Dr. Roberta K. Merkle, CCRC, 220 Riverbend Road, The University of Georgia, Athens, Georgia 30602-4712. Phone: 706-542-4402. FAX: 706-542-4412. E-mail: rmerkle@uga.cc.uga.edu ****************************************************************************** From: news@chaos.kulnet.kuleuven.ac.be Date: Sat, 22 Mar 1997 15:29:40 +0100 Subject: Re: peptide software Organization: K.U.Leuven Guenter Eigendorf wrote: } } I am looking for PC based software to calculate amino acid compositions } (natural and modified) for individual mass peaks in a peptide spectrum. } Does anybody know of a source. } } Guenter Eigendorf } Tel: 604 822-3235 } Fax: 604 822-2847 } e-mail: eigen@chem.ubc.ca Dear Guenter, A time ago I wrote some simple software to facilitate the interpretation of peptide MS/MS data. It runs under the good old DOS. It can also be used for composition calculations and unnatural residues can be included as well. Check the MINP= parameter. The source and compiled version of the program PEPSEQ can be found at http://www.farm.kuleuven.ac.be/farm/medchem/mass.htm If you have problems, contact me. Success, Jef. Jef Rozenski Rega Institute for Medical Research mailto:Jef.Rozenski@Rega.KULeuven.ac.be http://www.farm.kuleuven.ac.be/farm/medchem/jr.htm ****************************************************************************** From: "Charles Metcalfe" Date: 22 Mar 1997 21:55:33 GMT Subject: Creating a structure database for HP6890 Organization: Metcalfe's I have an HP6890/5972 GC-MS running Chemstation. I need to know how to create a database of structures to compliment the MS database I am creating. I use ISIS Draw (Molecular Design) to create the structures. HP is of no help, since I refuse to pay the yearly $1000 software support. Any help will be appreciated. ****************************************************************************** From: "Colin Cook" Date: Mon, 24 Mar 1997 09:02:20 +1100 Subject: Re: Isotopic internal standards Organization: * } } }I am looking for a supplier of internal standards for use in a mass } }spec screening procedure for pesticide residue analysis. I am after } }deuterated or 13C pesticides. An organochlorine or organophosphate } }would be best, but others are OK. I cannot find any supplier in my } }extensive collection of catalogs. } } }Colin Cook } Have you tried Supelco a now a division of Sigma Aldrich } Sigma-Aldrich Pty. Ltd. } Unit 2, 14 Anella Ave. } Castle Hill NSW 2154 } tel: 02 9841 0555 } 1-800-800 097 } fax 1 800 800 096 } 02 9841 0500 } } They supply deuterated standards of a number of compounds. } Ron } I've had a look through their catalog. They mainly supply surrogate standards for US EPA methods. The compounds they use don't seem to be too applicable to my method. I am looking for a pesticide that more closely resembles the compounds I am analysing. Colin Cook Section Leader Dept Natural Resources & Environment State Chemistry Laboratory Melbourne, Australia Ph (+61 3) 9742 8753 ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: ms of a new natural product. Organization: JEOL USA, Inc. Date: Mon, 24 Mar 1997 16:21:23 GMT In <5gs4cc$dvt@acmey.gatech.edu> Shai RUBNOV writes: }Hello every one. }I have a new natural product isolated from a n israeli plant. It has a }very strong pick at 84 m/z. It think it might be a piperidine alkaloids. }Does any one knows something about ms of such natural products? }Thanks in advance. }Shai Rubnov A peak at m/z 84 could indeed be C5H10N, indicative of piperidines. There are a number of other common compositions for a peak at m/z 84, including C6H12 (alkanes), C4H4S, C4H5O2, and a number of halogenated compositions. It is hard to base an interpretation on a single peak, but at least the m/z 84 peak lends some support to a piperidine structure if you have other support for this assignment. Good luck! -- ========================================================================= |____________ |_ Robert B. Cody, Ph.D |________________________________ Applications Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ |__________ http://www.jeol.com |_ e-mail: cody@jeol.com ========================================================================= ****************************************************************************** From: Corinne Bure Date: Mon, 24 Mar 1997 17:52:13 +0100 Subject: Recherche These MS France Organization: Grolier Interactive Europe Je suis actuellement en D.E.A de Spectrochimie et je recherche un sujet de Thèse dans le domaine de la Spectrométrie de Masse. Si une entreprise ou un laboratoire universitaire pense avoir besoin de mes services n'hésitez pas à me contacter. Vous trouverez plus de renseignements a mon sujet dans mon C.V ci-dessous. Corinne BURÉ 31/33, rue de Neuilly 92110 CLICHY. 01.41.06.08.18. Email cbure@club-internet.fr Célibataire, 24 ans. FORMATION SCOLAIRE: - 1997 : D.E.A. de Spectrochimie, Analyse et Physicochimie Organiques ( en cours ). Options : Spectrométrie de Masse et Chromatographie. Faculté de PARIS VI. - 1996 : Maîtrise de Chimie, Mention Assez Bien. Option : Spectrochimie Structurale et Modélisation. Faculté de ROUEN ( 76 ). - 1995 : Licence de Chimie. Faculté de ROUEN ( 76 ). - 1993 : D.U.T. de Chimie. Spécialisation : Biochimie Analytique. I.U.T. de ROUEN ( 76 ). - 1991 : Baccalauréat C. EXPÉRIENCES PROFESSIONNELLES: - 1997 : Stage d’étude dans le cadre du D.E.A. ( en cours ). Laboratoire de Spectrométrie de Masse de l’ENSCP, PARIS V. Analyse de l’huile essentielle de Patchouli par couplage GC-MS. - 1996 : Stage d’étude dans le cadre de la Maîtrise et emploi saisonnier, 3 mois. Société JANSSEN-CILAG, VAL DE REUIL ( 27 ). Analyse de différentes molécules par Résonance Magnétique Nucléaire. - 1993 à 1995 : Emploi saisonnier, 3x2 mois. Laboratoire d’Analyses Médicales FORAY et TARDY, BARENTIN ( 76 ). Hématologie, Immuno-Enzymologie et Biochimie du sang. - 1993 : Stage d’étude dans le cadre du D.U.T., 2 mois. Société VALENTINE, GRAND-QUEVILLY ( 76 ). Modification de la formule d’une pâte à teinter. RENSEIGNEMENTS DIVERS: - Anglais lu, écrit et parlé. - Notions d’Allemand. - Utilisation de logiciels bureautiques et de modélisation ( Sybyl sous Unix ). - Permis B et voiture. ****************************************************************************** From: ewichris@aol.com (EWI Chris) Date: 24 Mar 1997 16:58:55 GMT Subject: gas purification or filtration Organization: AOL http://www.aol.com Is gas purification or filtration a design consideration in spectroscopy or spectrometry equipment of any kind? (Mass, Liquid, X-ray, etc.) My company develops gas purifiers, scrubbers and filters for laboratory, pharmaceutical and medical OEMs. I'm looking for applications in spectrometry and related equipment. Thanks for the replys. Chris Dragotta M.W. Technologies, Inc. 71 Midland Ave. Elmwood Park, NJ, 07407 (201) 797 0700 Ph (201) 797 0745 Fx ****************************************************************************** From: Dr Rocky de Nys Date: 25 Mar 1997 00:15:39 GMT Subject: HP MSD (5971) Unstable AMU gain Organization: University of New South Wales Dear Newsgroup, The AMU gain after repeated tuning a 5971 MSD is highly variable after either venting to change a column or cooling the interface, to change an injection port liner. The variation is upto 150 units above the average tune setting of 360 but decreases over about 3 days to return to the average value. Symptom of drift in the gain value is a decrease in sensitivity during the course of running a sample sequence. After retuning sensitivity returns. I have not observed this variablility in AMU gain in other MSDs. Normally I can vent, change a column, tune, and have the MSD operational in 2 to 3 hours. Could someone please provide advice on what components maybe causing this problem and/or further diagnostic tests? Tim Charlton ****************************************************************************** From: "Little, James" Date: Tue, 25 Mar 1997 08:53:33 -0500 Subject: Creating a structure database for HP6890 Organization: Metcalfe's In response to "Charles Metcalfe" : }I have an HP6890/5972 GC-MS running Chemstation. I need to know how to }create a database of structures to compliment the MS database I am }creating. I use ISIS Draw (Molecular Design) to create the structures. HP }is of no help, since I refuse to pay the yearly $1000 software support. }Any help will be appreciated. We have a mass spectral database of 30,000+ spectra and 17,000 structures of components in our Corporate Electron Impact Database that we distribute to our users on CDROM. I figured out a way to create a structural database and associate it with the mass spectra that is compatible for searching on HP data systems. Send me your address and I will mail you a copy. James Little Eastman Chemical Company Kingsport, TN 37662 423-229-8685 ****************************************************************************** From: RAMSAY.RICHMOND@pharma.Novartis.com Date: Fri, 21 Mar 1997 20:00:14 +0100 Subject: Molecular ion cluster simulation in BASIC or V-BASIC code. Organization: Novartis I am interested in finding a BASIC or VISUAL BASIC program for simulating molecular ion clusters i.e. m/z values and % RA. I am interested in using this for colour rendering of ion current that belongs to an expected stoichiometric formula in less than 100% pure electrospray mass spectra i.e. from synthetic chemistry labs. Can anybody help out with suggestions or possible contacts ? In anticipation, your appreciatively, Ramsay Richmond ****************************************************************************** From: marc.gorenstein@mci2000.com (Marc Gorenstein) Date: Tue, 25 Mar 1997 22:11:03 GMT Subject: History of electrospray and multiple charging Organization: Shore.Net/Eco Software, Inc; (info@shore.net) I am interested in the history of electrospray and in the phenomenon of multiple charging. In particular, I am looking for references to the invention of electrospray. These can be references to the literature or to theses. I'm also looking for the initial published evidence that electrospray can produce a distribution of multiply charged analytes. I am new to the field. The important early work is usually the most interesting and useful to read. Thanks, Marc Gorenstein, Ph.D. Senior Scientist Waters Corporation Voice: (508) 482-2687 FAX: (508) 482-2055 ****************************************************************************** From: cody@jeol.com (Chip Cody) Subject: Re: History of electrospray and multiple charging Organization: JEOL USA, Inc. Date: Wed, 26 Mar 1997 16:44:30 GMT In <5h9jk7$jvo@acmez.gatech.edu> marc.gorenstein@mci2000.com (Marc Gorenstein) writes: }I am interested in the history of electrospray and in the phenomenon }of multiple charging. }In particular, I am looking for references to the invention of }electrospray. These can be references to the literature or to theses. }I'm also looking for the initial published evidence that electrospray }can produce a distribution of multiply charged analytes. }I am new to the field. The important early work is usually the most }interesting and useful to read. }Thanks, }Marc Gorenstein, Ph.D. }Senior Scientist }Waters Corporation }Voice: (508) 482-2687 }FAX: (508) 482-2055 Here are a few very early references: 1. M. Dole, L.L. Mach, R.L. Hines, R. C. Mobley, C.D. Ferguson, and M.B. Alice,J. Chem. Phys. 49, 2240 (1968) 2. L.L. Mach, P. Kralik, A. Rheude, M. Dole, J. Chem. Phys. 52, 4977 (1970). Dole is usually credited with the first work on electrospray. See also, 3. T.A. Milne, Int. J. Mass Spectrom. Ion Phys. 3,153 (1969) 4. E. Neher, M.S.Thesis, University of Wisconsin (1967) (Lysozyme) The recent wave of interest began with the explanation of how to interpret the mass spectra of multiply charged ions, presented by Mathias Mann, Chin Kai Meng, and John B.Fenn at the 36th ASMS Conference in San Francisco (abstracts, p. 1207). This was followed by a publication by the same three authors in Analytical Chemistry, v.61, 1702-1708 (1989). ========================================================================= |____________ |_ Robert B. Cody, Ph.D |________________________________ Applications Manager |__ Mass Spectrometry |________________________ JEOL USA, Inc. |_ |__________ http://www.jeol.com |_ e-mail: cody@jeol.com ========================================================================= ****************************************************************************** From: Peter Ockenfels Date: Wed, 26 Mar 1997 21:42:02 +0100 Subject: edwards foreline pump Organization: University of Freiburg Hi, does anyone has an idea to get an edwards foreline pump working again? After a routine change of the oil the pump will not start. The pump is connected to a HP GC/MS system (5890/5972) and about two years old. For me it seems that sedimentation inside the pump is the reason. May be there is somebody out there who has experience in opening and cleaning the pump mechanism. Any hints are welcome !! Cheers Peter -- .... Dr. Peter Ockenfels Forstzoologisches Institut der Universitaet Freiburg Aussenstelle Wittental Fohrenbuehl 27 79252 STEGEN .... ****************************************************************************** From: Scott Rando Date: Thu, 27 Mar 1997 09:31:06 -0500 Subject: Re: edwards foreline pump Organization: Warwick Online Peter Ockenfels wrote: }does anyone has an idea to get an edwards foreline pump working again? }After a routine change of the oil the pump will not start. The pump is }connected to a HP GC/MS system (5890/5972) and about two years old. For }me it seems that sedimentation inside the pump is the reason. }May be there is somebody out there who has experience in opening and }cleaning the pump mechanism. } }Any hints are welcome !! } }Cheers }Peter }-- }.... }Dr. Peter Ockenfels }Forstzoologisches Institut der Universitaet Freiburg }Aussenstelle Wittental }Fohrenbuehl 27 }79252 STEGEN 1) Some of the electric motors used on Edwards pumps have thermal shut down switches mounted on the end of the motor.  If so, make sure the button is pushed in to reset. 2) Does motor get very hot when you try to start the pump?  If not, there may be no power to motor. (Fuse,etc)  Don't let motor get too hot if it is not turning, it will draw excessive current and damage the motor. If neither of these things are the case, then the problem is mechanical.  Either the pump or the electric motor is at fault. Hope this helps, Scott ****************************************************************************** From: "Brandau, Ken" Date: Thu, 27 Mar 1997 11:03:55 -0500 Subject: PCB Sample Cleanup Organization: * We have PCB samples with incredibly nasty chemical matrices: electrical cable jackets, various felts, paint chips, etc. All are off old ships. SW-846 methods 3550B and 3620 for sulfuric acid reduction followed by florisil cleanup have been ineffective in removing some major interferences [phthalates in particular]. Has anyone had experience with better methods of cleaning up such samples? Any input would be most appreciated! We have considered gel permeation chromatography; however, management is inflexible on the prohibitive cost of such system. Thanks in advance! Ken Brandau ****************************************************************************** From: "Mark A. Hanning-Lee" Date: Thu, 27 Mar 1997 16:08:03 -0800 Subject: Re: PCB Sample Cleanup Organization: Prodigy Internet You might re-post this query to the wider chemical audience of the CHMINF-L list. Just send it to CHMINF-L@IUBVM.UCS.INDIANA.EDU. To subscribe send "subscribe CHMINF-L" to LISTSERV@IUBVM.UCS.INDIANA.EDU. See the list archives at http://www.chemistry.uakron.edu/CHMINF/. Best, Mark -- Mark A. Hanning-Lee, markhl@prodigy.net ****************************************************************************** From: Paul_Peterman@nbs.gov (Paul Peterman) Date: Mon, 31 Mar 1997 15:08:06 -0700 Subject: VG/Fisons 70 Magnet Cooling Line Connector Organization: * Has anyone repaired breakage of a water cooling line connector protruding from the electromagnet on a high resolution Mass Spec, preferably on a VG/Fisons Model 70 system? Thanks in advance, Paul Peterman USDI/USGS/BRD Midwest Science Center Columbia, MO ****************************************************************************** From: "F.A. Pinkse" Date: Tue, 01 Apr 1997 13:48:45 +0200 Subject: Re: edwards foreline pump Organization: Academic Computer Services Amsterdam (SARA) Peter Ockenfels wrote: } } Hi, } does anyone has an idea to get an edwards foreline pump working again? } After a routine change of the oil the pump will not start. The pump is } connected to a HP GC/MS system (5890/5972) and about two years old. For } me it seems that sedimentation inside the pump is the reason. } May be there is somebody out there who has experience in opening and } cleaning the pump mechanism. } } Any hints are welcome !! } } Cheers } Peter } -- } .... } Dr. Peter Ockenfels } Forstzoologisches Institut der Universitaet Freiburg } Aussenstelle Wittental } Fohrenbuehl 27 } 79252 STEGEN } .... Hallo Peter, I think you must try to do the following: Unplug the pump from the mains and try turning the pump shaft manually. I use the fan blades to do it, note the inlet must be open, this is important, you can not turn the pump manually against 1 bar!!!!. If the pump can be turned. Check the thermal cutout switch. Pressing it firmly. If the motor is still not running, the centrifugal switch may be stuck in the 'running' position. With some type of motors you can see the switch through the rear venting port. Pushing it with a screwdriver, only when the pump is unplugged. This switch isolates the start up coils when the motor reaches the running speed. If the switch operates smoothly and the contacts are not heavily burnt then the capacitor may need to be replaced. Just measuring its capacitance is not always succesfull. If the above is far to technical for you I would advice you to see for an electric technician who has experience with electric motors. Frans Pinkse, Institute of Mass Spectrometry ****************************************************************************** From: David Vollmer Date: Tue, 01 Apr 1997 13:54:38 -0700 Subject: SIS Filaments Organization: * To Finnigan Users, Recently we have been having problems with several lots of rebuilt Finnigan filaments purchased from SIS. Their service representative indicates this could be a problem with the tension. Has anyone else had similar problems? If so, is this problem due to the tension applied on the contact welds or maybe a poor quality of filament wire? Also, does anyone know of other filament repair services? Thanks in advance. David Vollmer ****************************************************************************** From: Bernhard Henkelmann Date: Tue, 01 Apr 1997 16:38:12 +0100 Subject: Re: Isotopic internal standards Organization: GSF Forschungszentrum fuer Umwelt und Gesundheit Colin Cook wrote: } } } } } }I am looking for a supplier of internal standards for use in a mass } } }spec screening procedure for pesticide residue analysis. I am after } } }deuterated or 13C pesticides. An organochlorine or organophosphate } } }would be best, but others are OK. I cannot find any supplier in my } } }extensive collection of catalogs. } } } } }Colin Cook } Try asking the Cambridge Isotope Laboratories, they have very much isotopic standards. You can get more information from their web-site: http://www.isotope.com ****************************************************************************** From: "Mark A. Hanning-Lee" Date: Tue, 01 Apr 1997 13:46:07 -0800 Subject: PC: digitizer, MCS, counter cards Organization: Prodigy Internet We want to select some fast cards which plug into a PC. We have requirements for 3 different products (see below). In each case I have looked around and identified some vendors, but would really appreciate any recommendations! 1: A waveform digitizer (transient recorder) card. This captures a waveform, digitizes it, and transfers it to the PC. Here are our main specs. o >=100 MS / s single-shot sample rate. o Record length (number of points per shot/sweep/scan) >= 5K, preferably 32K. o Signal averaging at a rate of 100 Hz min. at 5K record length, preferably 500 Hz at 5K, 250 Hz at 10K, etc. [If shots are stored sequentially, memory will fill up at >=0.5 M points/s.] o Cost not to exceed $4K on min. configuration and not to exceed $5K with enhanced performance. o 8 bit vertical resolution. o card should add (average) all the shots and transfer the sum. However, I have found no cards with on-board averaging in this price range. o 1 input channel. In the future we might need 2 or more though I cannot see an immediate need. o If we take shots at 200 Hz, and want a 1 s response, then expect to sum 200 - few thousand shots. o Post- and pre-trigger data recording might help, in case the most suitable trigger arrives early or late. This will enable us to gather just the points we want and reduce the amount of data handled. 2: A multi-channel scaler card which should count pulses with a time resolution of about 10 ns. 3: A simple counter card: o Accepts start and stop signals. o Count rate > ~1 MHz. o Can set the discriminator level to decide how big a pulse must be before it is counted. o Pulse pair resolution 10 ns; <= 50 ns OK. These general requirements apply to any card we buy: o Prefer BNC connectors. o free trial period preferred. o special support for OEMs like us who put card into an end product. o The more LabVIEW support the better! o We will use a Pentium but in the short term may develop on a 486. Thanks for listening, and I look forward to your replies. Mark -- Mark A. Hanning-Lee, markhl@prodigy.net ****************************************************************************** From: wandres@t-online.de (Britta Wenz) Date: 2 Apr 1997 17:23:06 GMT Subject: pyrethroide Organization: Telekom Online Internet Gateway Hallo, wer kann mir bzgl. der Bestimmung von Pyrethroide im biologischem Material helfen. Vielen Dank schon mal Britta Wandres ****************************************************************************** From: Tomasz Lojewski Date: Thu, 03 Apr 1997 14:43:12 -0800 Subject: jet separator tips? Organization: Faculty of Chemistry J.Univ. Cracow Hi, I am looking for some hints on the use of a jet separator. I am using an atmospheric pressure apparatus for thermal desorption experiments with QMS (VG SX-200 with rotary and turbomelecular pumps) as a on-line detector. I always use helium as a carrier gas (~20ml/min). Is it really usefull tool for interfacing normal pressure to vaccum? How it should be pumped? What gain I could expect for rather light molecules like NH3, NO or CO2? Any help would be appreciated. Tomasz Lojewski Chemistry Dep., Jagiellonian University, Poland ****************************************************************************** From: "James Selka" Date: 3 Apr 1997 12:46:16 GMT Subject: High Vacuum Compatible Precision Mechanical Components & sub Assemblies Organization: Reliance Gear Company Limited Anyone interested in High Vacuum Compatible Components and Sub Assemblies or Precise Gear Mechanisms can view them on the RELIANCE GEAR stand No. E16 in the British Pavilion Hall 26 at the Hanover Messe between April 14 to 19. For further information please see http://www.reliance.co.uk/ Fall Sie sich fuer Hochvakuum-Kompatible Bauteile und -Gruppen oder fuer Praezisions-Getriebe interessieren, wuerde RELIANCE GEAR Sie gerne im Rahmen der Hanover-Messe, vom 14.-19. April 1997, ueber diese Produkte informieren. Sie finden uns am Stand Nr. E16 im British Pavilion, Halle 26. Weitere Informationen koennen Sie dem Internet entnehmen. http://www.reliance.co.uk/ Reliance Gear Company Limited Rowley Mills Penistone Road Lepton Huddersfield West Yorkshire HD8 0LE Tel: +44 (0) 1484 601000 Fax: +44 (0) 1484 601001 Email: sales@reliance.co.uk ****************************************************************************** From: jonesy@chemiris.labs.BrockU.CA (Tim Jones) Date: Thu, 3 Apr 1997 10:01:05 -0500 Subject: Kratos/Spectros spares Organization: * Hello everyone. With our Concept 1S system perking along in its seventh year of operation, we have de-commissioned our old MS-30's and have a variety of spares available to anyone who is interested (Is anyone still using an MS-30 out there?). The items include a variety of MS-30 source spares including filaments and heaters, a complete source flange assembly, introduction locks and seals, Roband power supplies etc. We also have a complete preprocessor acquisition unit, Eclipse interface card and and Eclipse ethernet card for file networking. Please contact me if you are interested and we can discuss prices. Tim _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Tim Jones _/ Coordinator, Analytical Services _/ Dept. of Chemistry, _/ Brock University, _/ St. Catharines, Ontario _/ CANADA L2S 3A1 _/ Phone: 905-688-5550 Ext. 3850/4172 _/ FAX: 905-682-9020 _/ e-mail: jonesy@chemiris.labs.BrockU.ca_/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ ****************************************************************************** From: MasCom@t-online.de (MasCom) Date: 4 Apr 1997 12:32:20 GMT Subject: Re: SIS Filaments Organization: Telekom Online Internet Gateway David Vollmer wrote: } } To Finnigan Users, } } Recently we have been having problems with several lots of rebuilt } Finnigan filaments purchased from SIS. Their service representative } indicates this could be a problem with the tension. Has anyone else had } similar problems? If so, is this problem due to the tension applied on the } contact welds or maybe a poor quality of filament wire? } } Also, does anyone know of other filament repair services? } } Thanks in advance. } } David Vollmer MasCom GmbH Norderoog 1 28259 Bremen Germany Tel. 0049 421 571031 Fax 0049 421 571032 Repairs different kinds of Filaments from Finnigan and other Companies. ****************************************************************************** From: Paul_Peterman@nbs.gov (Paul Peterman) Date: Fri, 4 Apr 1997 10:51:20 -0700 Subject: Re: jet separator tips? Organization: * Tomasz Lojewski from Faculty of Chemistry J.Univ. Cracow wrote: }Hi, } }I am looking for some hints on the use of a jet separator. }I am using an atmospheric pressure apparatus for thermal desorption }experiments with QMS (VG SX-200 with rotary and turbomelecular pumps) }as a on-line detector. I always use helium as a carrier gas }(~20ml/min). Is it really usefull tool for interfacing normal }pressure to vaccum? }How it should be pumped? What gain I could expect for rather light }molecules like NH3, NO or CO2? }Any help would be appreciated. } }Tomasz Lojewski }Chemistry Dep., Jagiellonian University, Poland } From my previous experience with using a jet separator with helium carrier gas for a packed GC column, I recall that it removed about 90 to 95% of the helium that typically flowed at 20 to 35 mL/min. Typically a separate roughing vacuum pump was set up just for the helium or effluent of the jet separator. For most other compounds, about 65 to 80% were passed through to the MS source. For me, these compounds included volatile organics such as chloroform, dichloromethane, and benzene. The separation efficiency depends mostly on the distance between the jet tips and the diameter of each tip relative to one another. The pumping speed of the vacuum system may also have some effect. Catalogs and application notes from manufacturers and repair companies such as SIS (mentioned in another message) used to supply efficiency curves for various flows of helium and sometimes offered several choices of jets. The higher the mass of the analyte compared to helium, the higher the transfer efficiency of the analyte. Unless the jet tips are designed to be close for small flows of helium, overall transfer of analytes will drop if helium drops significantly below 20 mL/min. Given your three examples, you should get most (perhaps 50 to 65%) of CO2 through the jet, some of NO (maybe 35%), and probably only 10 to 15% of NH3. Good luck. ****************************************************************************** From: wandres@t-online.de (Britta Wenz) Date: 4 Apr 1997 18:03:58 GMT Subject: Pyrethroids Organization: Telekom Online Internet Gateway Does anyone have any ideas how one might go about detecting pyrethroids in blood or other biological materials by using GC/MS? Please help me Thank you email: wandres@t-online.de ****************************************************************************** From: nigel.osborn.zetnet.co.uk@irk.zetnet.co.uk (Nigel J.Osborn) Date: Fri, 04 Apr 1997 20:21:41 -0100 Subject: Contract Electrospray/MALDI work Organization: Amersham, Bucks I'm looking for anyone willing to analyse some protein samples using either MALDI or electrospray MS esp. LC-electrospray preferentially in the U.K. but elsewhere if UK not possible. I am prepared to pay a reasonable rate for the service . Anyone help? ****************************************************************************** From: PAREESDM@apci.com Date: Fri, 4 Apr 1997 17:24:23 -0500 Subject: Re: jet separator tips? Organization: * }I am looking for some hints on the use of a jet separator. }I am using an atmospheric pressure apparatus for thermal desorption }experiments with QMS (VG SX-200 with rotary and turbomelecular pumps) }as a on-line detector. I always use helium as a carrier gas (~20ml/min). }Is it really usefull tool for interfacing normal pressure to vacuum? yes }How it should be pumped? with a good quality roughing pump }What gain I could expect for rather light molecules }like NH3, NO or CO2? Well. you have a good point. These low molecular weight species would have the smallest advantage (other than hydrogen!). Still, it is really a (MW)^1/2 relationship, so even for ammonia, there is a factor of about 2. That's not really a lot. If I remember things I was told many years ago that light gases such as nitrogen had losses at the jet separator of about 75%. So 25% would be detected. That's not too bad, given that typically, about 98% or so of the helium is supposedly pumped away. This seems better than one should expect by looking at the square root of the ratio of the molecular weights. Does anyone else out there know anything more definitive on this subject. I do know I have successfully used jet separators for light gases. But I'm not sure of the enrichment factors. Dave Parees this opinion (guess) is not the responsibility of my employer }Any help would be appreciated. }Tomasz Lojewski }Chemistry Dep., Jagiellonian University, Poland }lojewski@trurl.ch.uj.edu.pl> ****************************************************************************** From: Peter Hunziker Date: Mon, 07 Apr 1997 11:03:59 +0200 Subject: Re: Contract Electrospray/MALDI work Organization: University of Zurich, Switzerland Nigel J.Osborn wrote: } } I'm looking for anyone willing to analyse some protein samples using either } MALDI or electrospray MS esp. LC-electrospray preferentially in the U.K. } but elsewhere if UK not possible. I am prepared to pay a reasonable rate } for the service . } } Anyone help? If you don't get an answer you may also have a look in the Yellow Pages of the ABRF (Association of Biomolecular Resource Facilities) home page. The mirror site in Cambridge can be reached at http://www.bio.cam.ac.uk/proj/adr/ABRF_mir/ABRF/YellowPages/96YellowPages.html They list 6 labs in UK who perform several types of analytical services. -- Dr. Peter Hunziker Universitaet Zuerich, Biochemisches Institut Winterthurerstr. 190 CH-8057 Zuerich, Switzerland Tel: 01/257 55 20 Fax: 01/363 79 47 E-mail: phunzi@bioc.unizh.ch ****************************************************************************** From: PAREESDM@apci.com Date: Mon, 7 Apr 1997 08:45:26 -0400 Subject: analysis of encapsulated gas Organization: * Does anyone know of an academic or commercial lab with the capability to analyze the gas contents of "tiny" bubbles in a polymer? I would especially appreciate labs in North America. I recall some work of this type (gas bubbles in arctic ice, studied for oxygen isotopic content to gain information about climate changes; industrial applications of inclusions in semiconductor chip coatings) some years ago, but not anything recently. Thanks, Dave Parees Air Products and Chemicals, Inc. 7201 Hamilton Blvd. Allentown, PA 18195 610-481-5794 pareesdm@apci.com ****************************************************************************** From: rgruber@ccnet.com Date: 7 Apr 1997 13:30:27 GMT Subject: SILICONE NMR Organization: CCnet Communications (510-988-7140 guest) HELP LOOKING FOR SOMEONE TO PERFORM SILICONE NMR. TRYING TO QUANTITATE THE SILICONE IN VARIOUS BIOLOGICAL SPECIMENS. WILLING TO PAY THE USUAL AND CUSTOMARY FEES. R GRUBER ****************************************************************************** From: "Crockett, Karen" Date: Fri, 04 Apr 97 11:52:00 PST Subject: HP Filaments Organization: * Hello out there! We have three HP GC/MS systems in our lab - two 5972s and one 5971. All three systems have dual filaments. On the 5971, when one filament burns out, the other one will still light. However, on both 5972 systems, when one burns out, the other one will not light. We have to replace the bad filament before the second one will light. I asked our HP service rep, but he didn't seem to know anything about it. Is anyone out there experiencing the same problem, and if so, what the fix is? Hope to hear from you. Thanks, Karen Crockett E-mail: kcrocket@ci.tacoma.wa.us Utility Services Engineering Laboratory City of Tacoma State of Washington, USA ****************************************************************************** From: "Crockett, Karen" Date: Fri, 04 Apr 97 11:52:00 PST Subject: HP Filaments Organization: * Hello out there! We have three HP GC/MS systems in our lab - two 5972s and one 5971. All three systems have dual filaments. On the 5971, when one filament burns out, the other one will still light. However, on both 5972 systems, when one burns out, the other one will not light. We have to replace the bad filament before the second one will light. I asked our HP service rep, but he didn't seem to know anything about it. Is anyone out there experiencing the same problem, and if so, what the fix is? Hope to hear from you. Thanks, Karen Crockett E-mail: kcrocket@ci.tacoma.wa.us Utility Services Engineering Laboratory City of Tacoma State of Washington, USA ****************************************************************************** From: gs_klinger@pnl.gov (George S Klinger) Date: Mon, 07 Apr 1997 21:27:03 +0000 (GMT) Subject: Re: Contract Electrospray/MALDI work Organization: Battelle Pacific Northwest National Labs (PN2L) We run both techniques in our lab. What information/experimental data are you after from these samples? Contact me is you'd like to discuss this further. George Klinger Sr Research Scientist Battelle Pacific Northwest National Laboratory (509)372-0448 gs_klinger@pnl.gov In article <5i3q8o$e9v@acmex.gatech.edu>, nigel.osborn.zetnet.co.uk@irk.zetnet.co.uk says... } }I'm looking for anyone willing to analyse some protein samples using either }MALDI or electrospray MS esp. LC-electrospray preferentially in the U.K. }but elsewhere if UK not possible. I am prepared to pay a reasonable rate }for the service . } }Anyone help? } } } } ****************************************************************************** From: Charlie Thibault Date: Tue, 08 Apr 1997 09:39:33 -0400 Subject: US, NY, Syracuse--Job:Scientific Software Developer Organization: Leybold Inficon, Inc Leybold Inficon, Inc., located in central New York State, is looking for a Scientific Software Developer responsible for developing and maintaining software under Microsoft Windows to process, display, and manipulate data collected by our environmental measurement equipment. This position reports to the HAPS Software Manager. The successful candidate must be an experienced, self-motivated individual with strong communication and team skills. Minimum educational and work experience is: a BS Chemistry, Physics, Computer Science or a related field, with at least 3 years experience or an advanced degree with 0-3 years experience. Both a background in Chemistry and substantial programming experience is essential. Technical experience is required in the following areas: - Windows programming experience (any flavor but Win-95 is prefered). - Programming experience in C and C++. - GUI programming, including menus, dialog boxes, buttons, sliders, etc. (preferably using Microsoft Foundation Class libraries). - Scientific programming, including the design and implementation of algorithms to process and interpret chromatographic and/or spectroscopic data. Technical experience in the following areas would be helpful: - Graphics programming, including experience with various techniques for graphical representation of data. - Familiarity with Visual C++ and/or Microsoft Foundation Class Libraries. - File formats, including experience with various formats for data storage and conversion between different formats. - Data transfer protocols, including standard protocols such as TCP/IP and the ability to develop simple proprietary protocols for specialized applications. Please send resumes and cover letters, including salary requirements, to: Leybold Inficon, Inc. Human Resources, Dept CT 2 Technology Place E. Syracuse NY 13057 -- [/^^\] Charlie Thibault cthibaul@inficon.com [ | ] HAPS Software Manager http://www.inficon.com [\|_/] Leybold Inficon, Inc. ChThibault@aol.com 2 Technology Place Phone: 315-434-1268 E. Syracuse NY 13057 Fax: 315-434-2520 ****************************************************************************** From: UDCA198@kcl.ac.uk Date: 8 Apr 97 15:57:43 GMT Subject: Re: SIS Filaments Organization: Kings College London In article <5i2un3$q5u@acmey.gatech.edu>, MasCom@t-online.de (MasCom) writes: } David Vollmer wrote: } } } } To Finnigan Users, } } } } Recently we have been having problems with several lots of rebuilt } } Finnigan filaments purchased from SIS. Their service representative } } indicates this could be a problem with the tension. Has anyone else had } } similar problems? If so, is this problem due to the tension applied on the } } contact welds or maybe a poor quality of filament wire? } } } } Also, does anyone know of other filament repair services? } } } } Thanks in advance. } } } } David Vollmer } } MasCom GmbH } Norderoog 1 } 28259 Bremen } Germany } } Tel. 0049 421 571031 } Fax 0049 421 571032 } } Repairs different kinds of Filaments from Finnigan and other Companies. I've used both the following firms to repair filaments for my KRATOS MS890 & JEOL AX505W. No problems have shown up: 1) Ash Instruments 5, Heather Close Phone: 01625 616431 Lyme Green Business Park Macclesfield Cheshire SK1 1OL UK 2) Filament Repair Service 45, Weavers Knowe Crescent Phone: 0131 449 2832 Edinburgh EH14 5PP UK Good luck! Andy Cakebread ****************************************************************************** From: TMB Date: Tue, 08 Apr 1997 15:59:13 -0500 Subject: metals speciation by ICP MS Organization: * Does anyone know of a commercial laboratory that can analyze soil to speciate the metals (specifically lead, zinc, aluminum, copper, nickel, and tin). I understand that it is done by ICP/MS. Thanks in advance for any responses. Theresa Brandabur ****************************************************************************** From: Gary Radford Date: Tue, 08 Apr 1997 21:09:35 -0400 Subject: Re: HP Filaments Organization: Radford Scientific Crockett, Karen wrote: } } Hello out there! } } We have three HP GC/MS systems in our lab - two 5972s and one 5971. All } three systems have dual filaments. On the 5971, when one filament burns } out, the other one will still light. However, on both 5972 systems, when } one burns out, the other one will not light. We have to replace the bad } filament before the second one will light. } See page 2-23 Fig 2-15 Top Board Block diagram in the 5971A Hardware manual. The -70 volt supply goes to both filaments. Since both filaments are tied togethter then if one of the filaments breaks and shorts out the source block then the -70v supply is shorted to ground. HP in order to save a few dollars in manufacture has a relay to select the filaament on one side. So if one filament just opens and doesn't short out to ground...the other filament will still work. They could just put a double pole relay to switch both sides of both filaments but they would probably want another $1000 for the unit. This could also happen on the 5971 even though you have only seen it on the 5972...Bad karma I guess ;-) } I asked our HP service rep, but he didn't seem to know anything about it. I quess they don't train them or pay them like they did before. } } Is anyone out there experiencing the same problem, and if so, what the fix } is? } } Hope to hear from you. } } Thanks, } Karen Crockett } E-mail: kcrocket@ci.tacoma.wa.us } Utility Services Engineering Laboratory } City of Tacoma } State of Washington, USA ****************************************************************************** From: 100202.3661@compuserve.com (Pierre Escoubas) Date: Wed, 09 Apr 1997 04:39:26 GMT Subject: Re: Contract Electrospray/MALDI work Organization: Suntory Institute for Bioorganic Research, Osaka, Japan }Nigel J.Osborn wrote: }} }} I'm looking for anyone willing to analyse some protein samples using either }} MALDI or electrospray MS esp. LC-electrospray preferentially in the U.K. }} but elsewhere if UK not possible. I am prepared to pay a reasonable rate }} for the service . }} }} Anyone help? Try ATHERIS in Switzerland http://www.atheris.ch/lab/ Cheers P. Escoubas Suntory Institute fro Bioorganic Research Osaka, Japan 100202.3661@compuserve.com ****************************************************************************** From: Jo Rita Jordan <76150.2171@CompuServe.COM> Date: 9 Apr 1997 14:04:35 GMT Subject: LC-MS users? Organization: Analytical Consumer Analytical Consumer is doing a survey of users of LC-MS, which was the focus of much interest at Pittcon last month. If you use LC-MS equipment and would be willing to answer a few questions, please send me an e-mail message. I'll send a copy of the report to anyone who participates. Thanks, Jo Rita Jordan Analytical Consumer ****************************************************************************** From: david_stranz@MassSpec.com (David Stranz) Date: Wed, 09 Apr 1997 15:53:34 GMT Subject: ASMS Computer Applications Interest Group Workshop Organization: Micromass Information Systems, LLC The Computer Applications Interest Group Workshop is scheduled for Wednesday, June 4, 5 - 6:30 pm. during the ASMS annual meeting in Palm Springs. The topic is yet to be determined, and I would like to receive suggestions and/or volunteers for participation. Last year's topic was Internet Resources for Mass Spectrometry, and the session was well attended and received. The basic ground rules are few: the ASMS wants the workshops to be informal, not a substitute for another oral session, so speakers should limit their comments to a few minutes and use overhead transparencies only. (Although if you have software to demo, we could arrange for a projector). The presentations also must be strictly non-commercial - you may speak about a commercial product, so long as your comments are confined to the scientific aspects of the product and not just a sales pitch. I am inclined to let this year's meeting be a "open mike" style session, but I open to suggestions for a more structured workshop. Please respond via e-mail to: david_stranz@MassSpec.com Thanks, David Stranz Computer Applications Interest Group Coordinator Micromass Information Systems, LLC ****************************************************************************** From: sjm@kore.co.uk (Steve Mullock) Date: Wed, 09 Apr 1997 15:16:10 GMT Subject: Re: PC: digitizer, MCS, counter cards Organization: Kore Technology Ltd. "Mark A. Hanning-Lee" wrote: }We want to select some fast cards which plug into a PC. We have }requirements for 3 different products (see below). In each case I have }looked around and identified some vendors, but would really appreciate }any recommendations! }1: A waveform digitizer (transient recorder) card. This captures a }waveform, digitizes it, and transfers it to the PC. Here are our main }specs. }o >=100 MS / s single-shot sample rate. }o Record length (number of points per shot/sweep/scan) >= 5K, preferably }32K. }o Signal averaging at a rate of 100 Hz min. at 5K record length, }preferably 500 Hz at 5K, 250 Hz at 10K, etc. [If shots are stored }sequentially, memory will fill up at >=0.5 M points/s.] }o Cost not to exceed $4K on min. configuration and not to exceed $5K }with enhanced performance. }o 8 bit vertical resolution. }o card should add (average) all the shots and transfer the sum. However, }I have found no cards with on-board averaging in this price range. }o 1 input channel. In the future we might need 2 or more though I cannot }see an immediate need. }o If we take shots at 200 Hz, and want a 1 s response, then expect to }sum 200 - few thousand shots. }o Post- and pre-trigger data recording might help, in case the most }suitable trigger arrives early or late. This will enable us to gather }just the points we want and reduce the amount of data handled. You probably know them already, but I suggest you look at the LeCroy digital oscillosopes. OK they won't fit in your PC but you might find that one fits the rest of your specs. and the data transfer can be quite quick down the IEEE interface. (Having a 'scope on an instrument can be pretty handy anyway) }2: A multi-channel scaler card which should count pulses with a time }resolution of about 10 ns. If you haven't already found us, we (Kore Technology) do a TDC/histogramming unit, usually used for time-of-flight mass spec. work. Please contact me if you'd like details and/or find further info on our web page http://www.kore.co.uk/users/ab48/ }3: A simple counter card: }o Accepts start and stop signals. }o Count rate > ~1 MHz. }o Can set the discriminator level to decide how big a pulse must be }before it is counted. }o Pulse pair resolution 10 ns; <= 50 ns OK. No suggestions, but I would be interested if you come across a counter card that can handle several (say 5 or 6) channels simultaneously. If you do, or anyone else knows of one, I would be most grateful for the lead. regards Steve -- Dr S.J. Mullock Kore Technology Ltd. Tel: 44 (0) 1223 420840 Fax: 44 (0) 1223 426041 http://www.kore.co.uk/users/ab48/ ****************************************************************************** From: "J.L. Beauchamp" Date: 9 Apr 1997 21:35:50 GMT Subject: Used/Junk TOF Mass Spectrometer Needed Organization: Caltech We are building an instrument that combines near field scanning optical microscopy with time of flight mass spectrometry to obtain chemical images of surfaces. If anyone has available a used/junk TOF Mass Spectrometer that they would like to part with it might be useful in our project. An early generation basic maldi instrument (e.g. Kratos, Finnigan, or similar) would be well suited for our application, especially with a short compact flight tube. Only modest resolution is required for our experiments. Thanks for any suggestions. ****************************************************************************** From: "Thomas, Brian" Date: Thu, 10 Apr 1997 08:30:40 -0400 Subject: LCQ vacuum problems Organization: Research Triangle Institute I recently purchased an LCQ from Finnigan/MAT and I have been suffering from rough pump failures when operating in APCI at a flow rate of 1 mL/min with acetonitrile/water mixtures. They contend that I am the only individual with this system that is having this problem and suggest that it must be the analytes that I am looking at. I think this is highly unlikely. So, I would appreciate hearing from any other LCQ users as to the "ruggedness" of their pumping system, or for that matter, the system as a whole. I would also like to hear from any TSQ or LCQ users regarding the ruggedness of the API probes. ****************************************************************************** From: rd_smith@ccmail.pnl.gov (Richard D Smith) Date: Thu, 10 Apr 1997 10:49:16 -0700 Subject: Positions available at Pacific Northwest National Laboratory Organization: Pacific Northwest National Laboratory We currently have Senior Research Scientist and Technical Specialist positions (both permanent staff) and three postdoctoral positions available in the new Environmental Sciences Research Laboratory at Pacific Northwest National Laboratory. The research program of the EMSL is developing and applying advanced instrumentation, including a high performance high magnetic field 12 tesla FTICR mass spectrometer, for biological applications. All positions are available in the near future. Sr. Research Scientist: Fourier Transform Ion Cyclotron Resonance Instrumentation and Methodology Development for Bioanalytical Applications. This position involves the development of advanced FTICR instrumentation and methods, with emphasis on the new high field 12 tesla FTICR. Specific efforts are aimed at development of more efficient external sources and trapping, advanced cell designs, and improved detection sensitivity and resolution. New methods based upon "individual ion" analysis are also being developed. Previous experience with FTICR is essential, and 2+ years of post doctoral or equivalent experience is required. The successful candidate must also be able to effectively work with a small team of researchers, must be self-motivated, and have good communication skills. Technical Specialist: Experience in the operation and maintenance of advanced FTICR instrumentation. Duties will include working with internal and external collaborators in the application of advanced FTICR instrumentation and techniques for biological applications. Post Doctoral Position: Development of on-line methodologies, including micro- fluidic, capillary electrophoresis and other methods, for the purposes of rapid and ultra-sensitive analyses of cellular constituents. This position will also involve the development and refinement of breadboard "front end" instrumentation for electrospray-mass spectrometry. Previous experience with micro-scale separation methods, LC-MS or CE-MS interfacing would be beneficial. Post Doctoral Position: Development of Advanced Methods for DNA Characterization. This position will involve the exploration of potential approaches using FTICR for high speed DNA sequencing based upon the analysis of Sanger-type mixtures, the characterization of PCR products, DNA damage and relevant DNA ion chemistry. Postdoctoral Position: Development/application of advanced mass spectrometric methods to the characterization of protein-protein and protein-DNA interactions. This position requires experience with protein purification and ligand binding studies desired, as well as previous experience with mass spectrometry. This position will involve a combination of activities, including collaborative research with outside investigators, and the interaction with other researchers at PNNL developing advanced Fourier transform ion cyclotron resonance mass spectrometry instrumentation and methods. Letters of interest should clearly specify the position of interest and relevant experience and be accompanied by a resume and list of references (with phone numbers) and should be addressed to me at the address indicated below. Dr. Richard D. Smith Pacific Northwest National Laboratory Mail Stop:P8-19 P.O. Box 999 Richland, WA 99352 Phone: (509) 376-0723 E-mail: rd_smith@pnl.gov ****************************************************************************** From: Jaroslaw Geldon Date: Fri, 11 Apr 1997 11:28:40 +0200 Subject: get practice Organization: * Dear Madam/Sir I am interested in course of ICP-MS and HPLC and get practice in your laboratory as well as courses concerning other methods. Could you send me more information if possible. I am PhD student. My sientific interest is enviromental chemistry. (distribution of heavy metals in bottom sediment of the marine ecosystems). I am coauthor of the following papers : 1.J.Ge³don, M.Chluœcik, A.Liwo, S.O³dziej, L.£ankiewicz and J.Ciarkowski. Modified Free-Wilson QSAR Analysis of [Leu5]Enkephalin Agonists. 2nd Israeli - Polish Symposium on the Chemistry and Biology of Peptides and Proteins pp 50. Rehovot , 4- 11 April 1995. 2. Szefer P., Kunio Ikuta, Seiji Kushiyama, Frelek K., Geldon J. (1997). Distribution of trace metals in Pacific oyster Crassostrea gigas and crabs from east coast of Kyushu Island, Japan. Bull.Environ. Contam.Toxicol. 58:108-114. 3. Szefer P., Kunio Ikuta, Seiji Kushiyama, Szefer K., Frelek K., Geldon J. (1997). Distribution and association of trace metals in soft tissue and byssus of Mytilus edulis from the east coast of Kyushu Island, Japan. Arch. Environ. Contam. Toxicol. 32:1-7. 4. Szefer P., Anis Ahmed Ali, Abdulla Bawazir, Mohamed Sad, Geldon J. (1997). Distribution and association of trace metals in soft tissue and byssus of Perna perna from the Gulf of Aden, Yemen. Environ. Intern. 23: 53-61. My address Jaroslaw Geldon Department of Analytical Chemistry Medical University of Gdansk ul. Gen. J. Hallera 107 Gdansk 80 - 416 Poland Fax 48 48 41 25 39 yours faithfully Jaroslaw Geldon ****************************************************************************** From: "Steven P. Cepa 847-937-7539" Date: Fri, 11 Apr 1997 08:11:00 -0500 (CDT) Subject: RE: LCQ vacuum Organization: * } "Thomas, Brian" writes: } I recently purchased an LCQ from Finnigan/MAT and I have been suffering } from rough pump failures when operating in APCI at a flow rate of 1 } mL/min with acetonitrile/water mixtures. They contend that I am the } only individual with this system that is having this problem and suggest } that it must be the analytes that I am looking at. I think this is } highly unlikely. So, I would appreciate hearing from any other LCQ users } as to the "ruggedness" of their pumping system, or for that matter, the } system as a whole. I would also like to hear from any TSQ or LCQ users } regarding the ruggedness of the API probes. We've had our LCQ since July '96 and have not had any pump failures. It is in daily use, mostly APCI, and usually at 1 mL/min with ACN:water. As a matter of fact, just this week it ran for 28 consecutive hours in APCI mode with a mobile phase of 65:35 MeOH:water. However, I agree with you that the analytes are not a likely cause of pump failure since there are only nanogram quantities being injected. Maybe you just picked the short straw and got a bad rough pump. What are your vacuum readings with the heated capillary capped off? The roughing gauge should be around 0.05 torr and the ion gauge about 1 x 10-5 torr. I have had trouble getting a good seal around the heated capillary where it connects to the spray shield. Finnigan did a redesign, but since you say you have a new LCQ, you should have the new design (different size o-rings: part #00107-10059 replaces old part #00107-05500; or 0.299 in ID replaces old 0.364 in ID Viton o-ring). -- Steve Cepa steven.cepa@abbott.com ****************************************************************************** From: Michael Balogh Date: Fri, 11 Apr 97 12:34:03 -0400 Subject: General Request for LC/MS Workshop Interests - ASMS '97 Organization: * The LC/MS and Related Topics Interest Group continues the effort of the past few years focusing on specific issues and enhancements in the field of liquid chromatography which have an impact on LC/MS practice. Related introduction techniques such as SFC and CE are also typically included. Many of the issues in the sample introduction and separation session raised last year will be revisited where significant advances have been made. Anticipated topics include column applications and novel hardware based on work in progress and not generally available to the public. Again this year a compilation of graphics and speaker transcriptions will be offered to attendees. The ASMS workshops are an informal means of sharing current developments in your area of interest with your colleagues. Speakers are encouraged to describe the highlights of their efforts in a few minutes using overhead transparencies only. Every effort is made to ensure the presentations remain non-commercial. Referring to particular products is, of course, unavoidable and often warranted and of value to the attendees - so long as your comments are confined to the scientific aspects of the product. The LC/MS and Related Topics Interest Group Workshop is scheduled for: Tuesday, June 3, 5 - 6:30 PM at the ASMS annual meeting in Palm Springs. If you are interested in presenting work or have any comments or suggestions please respond to me by e-mail at: usmpb@waters.com Michael P. Balogh Waters, LC/MS ****************************************************************************** From: "Tomas Öberg" Date: 11 Apr 97 16:30:21 GMT Subject: Experimental design and optimization software Organization: Bergström & Öberg MultiSimplex, a new software for sequential experimental design and optimization, is now released. Visit http://www.multisimplex.se or write info@multisimplex.se Best regards, Tomas Oberg ****************************************************************************** From: Paul_Peterman@nbs.gov (Paul Peterman) Date: Fri, 11 Apr 1997 16:04:30 -0600 Subject: VG Magnet - Water Cooling Connector Repaired Organization: * Several weeks ago, I wrote: }Has anyone repaired breakage of a water cooling line connector }protruding from the electromagnet on a high resolution Mass Spec, }preferably on a VG/Fisons Model 70 system? }Thanks in advance, }Paul Peterman }USDI/USGS/BRD }Midwest Science Center }Columbia, MO Originally, the connector was soldered to the internal (1/4" or 6 mm) copper water line, but we wanted to avoid resoldering it because there was no ready access and sufficient heat near the internal wiring might have damaged something else. Apparently no spare coils or complete magnets for this instrument exist. Thankfully, colleagues (including Carl Orazio, John Meadows, Jim Zajicek, and Robert Gale) as well as local machinists and VG service specialists (from Research Scientific Services in North Carolina) brainstormed and made initial "dry-run" tests with two different epoxy resins. The epoxy that was chosen by all and was successful was J.B. Weld! ****************************************************************************** From: Christopher Kaine Date: Fri, 11 Apr 1997 17:44:08 -0700 Subject: Re: LCQ vacuum problems Organization: OneNet Communications HUB News Server Thomas, Brian wrote: } } I recently purchased an LCQ from Finnigan/MAT and I have been suffering } from rough pump failures when operating in APCI at a flow rate of 1 } mL/min with acetonitrile/water mixtures. They contend that I am the } only individual with this system that is having this problem and suggest } that it must be the analytes that I am looking at. I think this is } highly unlikely. So, I would appreciate hearing from any other LCQ users } as to the "ruggedness" of their pumping system, or for that matter, the } system as a whole. I would also like to hear from any TSQ or LCQ users } regarding the ruggedness of the API probes. You have not really specified how many pumps have failed, or where your lab is located(in the US or not, i.e. are you dealing with Finnigan directly, or going through a dealer). The forepumps which Finnigan has been using for the recent past have been very reliable. Perhaps as previously mentioned, you unfortunately just got a bad one. ****************************************************************************** From: chrisjcr@aol.com Date: 12 Apr 1997 08:44:41 GMT Subject: Chromatography Conference Prelim. Program Organization: AOL http://www.aol.com This is a reminder of the upcoming chromatography conference to be held in Greenville, South Carolina, USA, on April 24, 1997. The preliminary program, as well as links to other chromatography related sites can be found on our web page at "http://members.aol.com/chromgroup". Technical Program 8:15 Registration and Exhibits 8:40 Filter Selection in HPLC Method Development, Mary Mayer, Gelman Sciences 9:00 Microwave Solvent Extraction Techniques for Various Regulatory Compounds, Rita Clement-Nowlin, CEM Corporation 9:20 New GC Techniques: Tools for Fast GC and Large Volume Injections, Liz Tierney, Shimadzu Instruments 9:40 A New Generation RP Stationary Phase-Purospher RP18e, Fred Rabel, EM Science 10:00 Break and Exhibits 10:40 Advances in the Analysis of Anions and Cations by Ion Chromatography, Dick Flynn, Dionex Corporation 11:00 KEYNOTE ADDRESS: Gas Chromatography: Older Misconceptions and Newer Advances, Prof. Walt Jennings, J&W Scientific 12:00 Lunch - Complimentary with ticket if preregistered 12:45 Exhibits 1:30 Modern Detection Techniques for HPLC, Tyler Tull and Vickky MyKyTyn, Waters Corporation 1:50 Special Separation Techniques, Fred Jensen, Perkin-Elmer/Sciex 2:10 A New Phase for Direct Injection of Biological Samples, Fred Rabel, EM Science 2:30 Using an ELSD as a Detector for Supercritical Fluid Extraction and Chromatography, Michael J. Drews, Jesse E. Helvey, and Kim Ivey, School of Textiles, Fiber, and Polymer Science, Clemson University 2:50 Break and Exhibits 3:10 Use of Small Diameter Columns in Analytical HPLC, Rich Smiechowski, YMC 3:30 Drawing for Door Prizes Exhibitors Air Products Daniel Scientific Dionex EM Science Gelman Sciences Hewlett-Packard J&W Scientific Jones Chromatography Krackeler Scientific Micromass National Welders Supply National Scientific Newcomb Associates Perkin-Elmer/Sciex Polymer Laboratories SGE Shimadzu Instruments Southeastern Technical Sales Southern Measurement Thermo Separation Products Varian Associates Waters Wesinco VWR Scientific YMC Let me know if there are any questions. Chris Richardson W.R. Grace, Cryovac Division (864) 433-3248 ChrisJCR@aol.com Chris.Richardson@corp.wrgrace.com http://members.aol.com/chrisjcr ****************************************************************************** From: A.Dietrich@em.uni-frankfurt.de (Armin Dietrich) Date: Sat, 12 Apr 1997 12:48:46 GMT Subject: Injector leak? GC3400 / ITS 40 Organization: J. W. Goethe-Universitaet Frankfurt/Main Can anyone help me? I have a problem with a "leak" in the injector system of a Varian GC 400, connected to a Finnigan MAT ITS 40. If I set the flow of the injector split and septum purge to "normal" flow rates (30 ml He split / 5 ml He purge), the MS indicates a leak system. (Ratio N2/H20/CO2 o.k, according to the manual) Increasing the septum purge to 60 ml, the system seems to be tight. (Ratio N2/H20/CO2 o.k, according to the manual) It looks as there would be a leak in the injector system, and air diffusion into the system via the upper part of the injector, but I can not find any leak. Has anybody experience with this GC-MS system and any ideas? Thanks!!! Armin ****************************************************************************** From: mrheadmstr@aol.com Date: 12 Apr 1997 05:35:05 GMT Subject: MIMS membranes? Organization: AOL http://www.aol.com I'd like to experiment with a membrane seperator as a means to enrich the flow from a TGA. Does anyone know where one can obtain the required membranes, which I understand to be silicone of about 20 microns thickness? TIA MrH ****************************************************************************** From: "javo" Date: Mon, 14 Apr 1997 09:08:46 +0200 Subject: Accelerating voltage on Finnigan Matt 8200 Organization: * Hi, Regarding the accelerating voltage of 3 kV on the BIC unit of the Finnigan Matt MS. With no warning or any symtoms the reading on the voltage meter seemed to have disapeared. The meter seems not to be at fault. Can anybody advise me where to start looking for the problem? Jan Vorster, Rand Afrikaans University South Africa, int. tel. 27 11 489 2358 e-mail: javo@rau1.rau.ac.za ****************************************************************************** From: Ivan Date: Mon, 14 Apr 1997 17:59:29 +0800 Subject: Urgent!!! Organization: Netvigator Can anyone tell me the fragementation pathway of sugars and effects on the fragementation of sugars in tandem MS in the presence of alkali metals or transition metals? Thanks for your answers! -- mailto:ivanyip@netvigator.com or mailto:ivanyip@usa.net ****************************************************************************** From: Unknown (FRP242) Date: 14 Apr 1997 11:09:22 GMT Subject: Electrospray contaminants Organization: IFR NL Does anyone know of a compilation of contaminants (especially polymers) commonly found in electrospray mass spectra of biological/biochemical extracts? Regards, Fred Mellon Institute of Food Research Norwich NR4 7UA UK ****************************************************************************** From: nathand@cco.caltech.edu (Nathan F. Dalleska) Date: 14 Apr 1997 20:05:13 GMT Subject: FAB on VG ProSpec Organization: California Institute of Technology Is there anybody out there running FAB (or LSIMS, if you prefer) on a ProSpec? I'm looking for someone to compare notes with. Please respond by email to nathand@cco.caltech.edu Thank you. Nathan -- Nathan Dalleska nathanzd@ccoz.caltech.edu remove zs to mail ****************************************************************************** From: Joerg.w@t-online.de.vv (J\vrg W\vhrmann) Date: 14 Apr 1997 19:39:42 GMT Subject: Combinatorial Chemistriy Organization: Telekom Online Internet Gateway Hello, is there anyone who can give me some Informations about Combinatorial Chemistriy, especially in connection with the use of LC-MS or GC-MS systems. ****************************************************************************** From: "Kiley R. Prilliman" Date: Mon, 14 Apr 1997 19:08:20 -0600 Subject: Re: Electrospray contaminants Organization: University of Oklahoma Health Sciences Center ...i'd be interested in the same! i don't think it would be a simple response to answer this question, though, as each individual's extraction technique may introduce completely unique sources of contamination... --Kiley ****************************************************************************** From: junti@utu.fi (junti) Date: 15 Apr 1997 10:25:57 GMT Subject: Re: Urgent!!! Organization: =?UNKNOWN-8BIT?Q?Per=E4kyl=E4?= In article <5it6qa$psh@acmey.gatech.edu>, Ivan says: } }Can anyone tell me the fragementation pathway of sugars and effects on }the fragementation of sugars in tandem MS in the presence of alkali }metals or transition metals? Thanks for your answers! }-- }mailto:ivanyip@netvigator.com or }mailto:ivanyip@usa.net } } Vanya, i ne stydno Vam zadavat' takie voprosy, da eshche s pometkoi "Srochno"?! Pochitaite literaturu. Chemical Abstracts naidite i pochitaite. ****************************************************************************** From: MasCom@t-online.de (MasCom) Date: 15 Apr 1997 14:08:36 GMT Subject: Re: Accelerating voltage on Finnigan Matt 8200 Organization: Telekom Online Internet Gateway javo wrote: } } Hi, } Regarding the accelerating voltage of 3 kV on the BIC unit of the Finnigan } Matt MS. With no warning or any symtoms the reading on the voltage meter } seemed to have disapeared. The meter seems not to be at fault. Can anybody } advise me where to start looking for the problem? } } Jan Vorster, Rand Afrikaans University } South Africa, int. tel. 27 11 489 2358 } e-mail: javo@rau1.rau.ac.za As a first step, you should disconnect the source cable. If the voltage comes back, you have a short circuit in the source. As a second step, you should measure the deflection voltage omn the BAI Unit. The deflection voltage controls the acceleration voltage. There are also some fuses to control at the BAi and BHI-Unit. Good luck. Bernhard Meyer ****************************************************************************** From: Valentin Date: Tue, 15 Apr 1997 15:32:52 +0400 Subject: Re: Resonant traps for particles. Part 2. Organization: Scientific-Research Center "IKAR" "There are the problems a solution of which in a singleton it is impossible and any veil of secret early or latly will be only to hinder them and us... " " It is impossible to grasp immense... " - Kuzma Prutkov - " But... Piecemeal and Together it is possible " - Scientific-Research Center "IKAR". Problems of levitation," live and dead " waters, chaos and evolution, discretization, self-steady resonance plasma formations... Theme of resonance action of various fields on nonlinear physical and biological systems on our home page - http://access.mark-itt.ru/ikar SRC 'IKAR' would be glad to cooperate with the researchers, who are interested in this technologies, in particular in technologies: -development of resonant traps where plasma is to be used; self-confinement plasma - ball lightning; -development of the new non-contact methods for prophylactics, treatment and diagnostics on the basis of effect of resonant fields on biological systems; biochemical and chemical reaction's control. We already have received interesting outcomes in these directions (please, contact us: tel. (341-2 )-211-760; E-mail: lina@ikar.udm.ru). ****************************************************************************** From: Date: 15 Apr 1997 14:29:57 GMT Subject: HP 5989B, ES, AP-CI Organization: Sprynet News Service A HP 5989B MS engine with neg ion detection, extended mass range, electrospray LS and AP-CI LS interfaces, ion guide and Neslab chiller, fully refurbed by HP itself, has just become available for $69K. Michael Sherrell 707 887 2919 fax 707 887 9834 ****************************************************************************** From: ada2@inel.gov (Anthony D Appelhans) Date: Tue, 15 Apr 1997 08:56:19 -0600 Subject: post doc Organization: * Postdoctoral Associate in Surface Chemistry Idaho National Engineering and Environmental Laboratory P. O. Box 1625 Idaho Falls, Idaho Applications for post-doctoral research in the area of surface chemistry are being solicited. The research will involve investigation of adsorption processes on both inorganic and organic substrates which occur under ambient conditions. The thrust of this research is to identify and/or develop surfaces which have enhanced selectivity for adsorption of specific or classes of analytes from air and aqueous environments. This project is part of a large research effort which centers around the application of static secondary ion mass spectrometry (SIMS) to detection and characterization of analytes on surfaces ranging from minerals to vegetation. In addition to static SIMS, other tools available for this research include electron spectroscopies (XPS and Auger) and molecular spectroscopies (IR and Raman). Desired qualifications include research interests in interfacial chemistry, familiarity with surface analysis (preferrably SIMS), and analytical chemistry. Additionally, knowledge of surface modification for selective adsorption would be useful. Candidates must be U. S. citizens. The appointment is for one year, renewable after review. Interested parties should contact Jani Ingram uoa@inel.gov, (208) 526-0739. Tony Appelhans Idaho National Engineering Laboratory P.O. Box 1625 Idaho Falls, ID 83415-2208 208 526 0862 FAX 208 526 8541 e-mail ada2@inel.gov ****************************************************************************** From: Paul_Peterman@nbs.gov (Paul Peterman) Date: Tue, 15 Apr 1997 10:07:12 -0600 Subject: Loss of Accelerating Voltage on High Res. MS Organization: * }On Date: Mon, 14 Apr 1997 09:08:46 +0200, "javo" wrote: }Subject: Accelerating voltage on Finnigan Matt 8200 Organization: * }Hi, }Regarding the accelerating voltage of 3 kV on the BIC unit of the }Finnigan Matt MS. With no warning or any symtoms the reading on the }voltage meter seemed to have disapeared. The meter seems not to be at }fault. Can anybody advise me where to start looking for the problem? }Jan Vorster, Rand Afrikaans University South Africa, int. tel. 27 11 }489 2358 e-mail: javo@rau1.rau.ac.za You need to first determine whether the high voltage board(s) and accompanying power supplies are supplying or can supply the 3 kV. So isolate that high voltage from the Mass spec flange connection, by removing the cable or connection to the mass spec ion source. If you don't have 3 kV, then further troubleshoot your electronics by tracing high voltage circuit board(s). If you now do have 3 kV, but lose the high voltage when the mass spec's ion source is connected, then the problem is likely that your MS ion source is dirty, especially the ceramics. When the ceramic parts which isolate your lenses get dirty, they shunt or leak voltage until eventually you might not be able to get any significant voltage. Flaming the ceramics or baking them in a muffle furnace may help but ideally, replace as many "expendable" ones as necessary. If you have access to a special "MEGO" electronic very high resistance device (above 20 megohms), you may be able to better pinpoint conductive ceramic parts. Good luck, Paul Peterman ****************************************************************************** From: vanreet@ilink.nis.za (Teunis vanRee) Date: 15 Apr 1997 17:29:07 GMT Subject: Re: Combinatorial Chemistriy Organization: Network Information Services J\vrg W\vhrmann (Joerg.w@t-online.de.vv) wrote: : Hello, : is there anyone who can give me some Informations about Combinatorial : Chemistriy, especially in connection with the use of LC-MS or GC-MS : systems. Joerg, I fail to see a direct connection, but here are some leading references. Use Chem Abs and SCI to follow up: General: Gallop, MA, et al. 1994. J Med Chem, 37, 1233-1251. Gordon, EM, et al. 1994 J Med Chem, 37, 1385-1401. Terrett, NK, et al. 1995. Tetrah, 51, 8135-8173. Thompson, LA & Ellman, JA. 1996. Chem Rev, 96, 555-600. Fruechtel, JS & Jung, G. 1996. Angew Chem Int Ed Engl, 35, 17-42. MS: Chen, C, et al. 1994. J Am Chem Soc, 116, 2661-2662. Zambias, RA. 1994. Tetrah Lett, 35, 4283-4286. Brummel, CL, et al. 1994. Science, 264, 399-402. Brummel, CL, et al. 1996. Anal Chem, 68, 237-242. GC: Ohlmeyer, MHJ, et al. 1993. Proc Natl Acad Sci USA, 90, 10922-10926. Nestler, HP, et al. 1994. J Org Chem, 59, 4723-4724. Baldwin, JJ, et al. 1995. J Am Chem Soc, 117, 5588-5589. HPLC: Ni, Z-J, et al. 1996. J Med Chem, 39, 1601-1608. Enjoy! Thomas ****************************************************************************** From: Drew Gibson Date: Tue, 15 Apr 1997 19:59:48 +0100 Subject: Re: Electrospray contaminants Organization: The Mad House Distribution: world On Mon, 14 Apr 1997 at 08:09:59, Unknown > wrote in Electrospray contaminants }Does anyone know of a compilation of contaminants (especially polymers) }commonly found in electrospray mass spectra of biological/biochemical }extracts? } }Regards, } }Fred Mellon If anyone /does/ have such a beast, could they post to the newsgroup rather than email the poster, as I'm sure a number of us are interested in this. Do you have a particular problem Fred, or just out of interest ? Regards from an ex- UEA MSc spectroscopic and physical methods student :) -- Cheers, Drew ;^) }}} drew@thegibsons.demon.co.uk <<< ****************************************************************************** From: YONG HUANG Date: Wed, 16 Apr 1997 10:26:26 -0500 (CDT) Subject: Laser shot counter Organization: * I'm thinking about electronically counting how many laser shots I use everyday to free me from manual logging. What's the cheapest way to do it? There's a SYNC OUT port (BNC connection) on the laser. Probably TTL type signal. Can I send it to my PC and count it? Any program has already been written for this? Thank you for help. BTW, I wonder why laser makers haven't thought about putting a counter in the laser. Yong ****************************************************************************** From: dave@spam_be_gone.org (dave's not here) Date: Wed, 16 Apr 1997 20:49:14 +0000 (GMT) Subject: Re: Laser shot counter Organization: Arizona State University YONG HUANG wrote: }I'm thinking about electronically counting how many laser shots I use }everyday to free me from manual logging. What's the cheapest way to do it? }There's a SYNC OUT port (BNC connection) on the laser. Probably TTL type }signal. Can I send it to my PC and count it? Any program has already been }written for this? Thank you for help. BTW, I wonder why laser makers }haven't thought about putting a counter in the laser. }Yong The Continuum SureLite I series of lasers has a built-in counter on the power supply. Every time you turn the power supply on, the display shows the accumulated number of times the flashlamps have fired. Assuming, perhaps incorrectly, that the trigger for your data system is the firing of the laser, you should be able to take this signal, and with either an external counter or a pc board (from national instruments or similar) sum the number of laser shots. Used counters (like an HP 5314 Universal counter) can be found for about $300. spam is not as tasty as bologna, so i can be reached at: dogruel@asuchm.la.axu.edu by changing the x to s. ****************************************************************************** From: blades@chem.ubc.ca (Mike Blades) Date: 16 Apr 1997 23:30:49 GMT Subject: FACSS Conference - Providence. R.I. - October 25th - 31st Organization: Chemistry, UBC 1997 Meeting of the Federation of Analytical Chemistry and Spectroscopy Societies (http://FACSS.org/) The 1997 Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) meeting will be held in Providence, Rhode Island - October 25 - 31, 1997. FACSS has broad coverage of topics within analytical chemistry and spectroscopy. Each year FACSS is the meeting where analytical scientists attend many talks outside of our own research areas. FACSS is known as arguably the world's best meeting for industrial applications of Raman spectroscopy. Academic atomic spectroscopists have long considered FACSS the conference they and their students must attend each year. Outstanding symposia include many different topical areas each year. FACSS is focused. The quality of symposia is excellent. Several program sections could stand on their own as focussed meetings. FACSS is a meeting for academics and students and FACSS is a meeting for industrial problem solvers and government scientists. FACSS is consistently the meeting where the leading academic, government and industrial analytical scientists and spectroscopists get together. Typically about 1700 conferees attend FACSS with more than 800 presentations. FACSS is big enough to thoroughly cover many different subject areas but small enough to make it easy to "bounce" from one symposium to another, make new scientific contacts and discuss new ideas. This year, in addition to molecular spectroscopy, atomic spectroscopy, mass spectrometry, electrochemistry, chemical separations, chemometrics and process control, there will be a special emphasis on biological, biomedical, pharmaceutical and environmental applications in more than 30 symposia. Also, three concurrent Plenary Lectures will be presented each day by some of the world's leading analytical chemists and spectroscopists (with no concurrent symposia). This will give conferees further opportunities to learn about cutting edge developments in a variety of areas. For more information on the FACSS conference and to submit a title before the deadline of April 30, 1997 connect to the FACSS home page at http://FACSS.org/ ****************************************************************************** From: sparus@umich.edu (Steve Parus) Date: Thu, 17 Apr 1997 13:02:49 GMT Subject: Re: Laser shot counter Organization: University of Michigan YONG HUANG wrote: }I'm thinking about electronically counting how many laser shots I use }everyday to free me from manual logging. What's the cheapest way to do it? }There's a SYNC OUT port (BNC connection) on the laser. Probably TTL type }signal. Can I send it to my PC and count it? Any program has already been }written for this? Thank you for help. BTW, I wonder why laser makers }haven't thought about putting a counter in the laser. I've used a miniature elecronic counter module to do this. Red Lion Controls (and other companies) make them. The Red Lion are available from Digi-Key at 800-344-4539 www.digikey.com. Model CUB7 is an 8 digit counter with LCD readout, replaceable lithium battery (7 year life), count rate to 10 kHz, $44, 1 inch x 2 inch. Digi-Key part # RLC700-ND sparus@umich.edu ****************************************************************************** From: shuster@socrates.berkeley.edu (David Shuster) Subject: GC/MS: Mass es 91, 106 Date: Thu, 17 Apr 1997 01:06:38 GMT Organization: University of California, Berkeley [This is forwarded from sci.chem.analytical - DB] I have a vacuum line inlet system attached to a gc/ms via a six port gas sampling valve. When I run blanks onto a phaseless column from my vacuum line, I see a small ethyl-benzene peak in my background. Does anyone have any ideas where this contaminant would be coming from? Baking out my line at 120 C does not appear to get rid of the contaminant. David Shuster shuster@socrates.berkeley.edu ****************************************************************************** From: "Mark A. Hanning-Lee" Date: Thu, 17 Apr 1997 13:46:31 -0700 Subject: Re: Laser shot counter Organization: Prodigy Internet YONG HUANG wrote: } } I'm thinking about electronically counting how many laser shots I use } everyday to free me from manual logging. What's the cheapest way to do it? } There's a SYNC OUT port (BNC connection) on the laser. Probably TTL type } signal. Can I send it to my PC and count it? If the pulses are TTL, then you have a wide choice. I have also been seeking cheap counter boards to plug into a PC, and found these companies and part #s. We have not yet ordered one. In each case browse the Web site or call the company and ask for the application engineer for data acquisition. * Computer Boards Inc. Cheap, catalogs usually compare their products to Keithley MetraByte. 125 High Street Mansfield MA 02048, (508) 261 1123, fax 1094, info@comp-boards.com. No web page. MetraByte spin-off. They have ~20 counters, all <10 MHz. Counters only count TTL and they have no discriminator cards. Most of their cards fit into PC/XT/AT/386 (i.e. ISA) bus. A few PCI, PCMCIA, & parallel port cards. All cards use 37-pin D connector; they sell no BNC cable adapters; try DigiKey 1-800-344-4539 for adapters. I think that they make their own cards. Catalog volumes 7 & 10: CIO-CTR05 has 8 digital inputs, 8 digital outputs, 5 16-bit (65536 counts) 7 MHz counter timers. Each timer uses an AM9513 chip; software can chain them together to emulate a 32, 48, 64, or 80 bit counter. 1 MHz clock crystal with 50 or 100 ppm accuracy. $139. To program this "complicated" chip, they recommend getting the 100-page 9513 manual! Catalog volume 9: CIO-DAS08 series, cheapest is CIO-DAS08, 8 analog inputs, 7 digital i/o, 3 16-bit Intel 8254 10 MHz down counters. <2 W, $199. Catalog volume 10: CIO-DIO24/CTR3 24 lines of digital i/o, same 3 counters, internal jumpers to chain counters, 2 W, $99. UniversalLibrary is a complete set of i/o tools $49. InstaCal is a installation, calibration, and test program free with every order. * Omega Engineering One Omega Drive, Stamford CT 06907-0047. 800-826-6342 or (800) 848-4286 or (203) 359-1660, fax 7700. Ask for data acquisition. http://www.omega.com/ information info@omega.com. Data acquisition cards <40 MHz. Counter/timer cards <10 MHz. They apparently have a card with 5 TTL counters plus digital i/o lines for $149. They may not make their own cards. * Intelligent Instrumentation sell counters with clock speeds <16 MHz. Tucson AZ, 1-800-685-9911, http://www.instrument.com/. Spin-off of the PC cards division of Burr-Brown. Apparently they make 85% of products in their catalog. * Analogic Corporation, 8 Centennial Drive, Peabody, MA 01960, (800) 446 8936 or (508) 977-3000 fax 6818. * Keithley MetraByte. 216-498-2938, fax 216-498-2990. Mostly ISA bus but will release PCI in 4/97. * Data Translation http://www.datx.com. Good luck, Mark -- Mark Hanning-Lee, markhl@prodigy.net ****************************************************************************** From: Achim Treumann Date: Fri, 18 Apr 1997 11:04:22 +0900 Subject: Re: MSMS of carbohydrates and metal ions Organization: Fujita Health University Ivan wrote: }Does anyone can tell me the effect on the fragementations }of sugar in the present of metal ions such as alkali metals }and transition metals, }thanks!!! Dear Ivan, In the following I assume that you are interested in CID fragmentation of carbohydrates in electrospray ionisation mass spectrometry. Vern Reinhold has done some impressive work on the analysis of methyl-derivatised oligosaccharides in the presence of sodium. Linsley KB, 1994 [See Related Articles] Applications of electrospray mass spectrometry to erythropoietin N- and O-linked glycans. Anal. Biochem. 219, 207-217 (1994) Reinhold VN, 1996 [See Related Articles] Carbohydrate sequence analysis by electrospray ionization-mass spectrometry. Meth. Enzymol. 271, 377-402 (1996) (no abstract available) Stroud MR, 1996 [See Related Articles] Monosialogangliosides of human myelogenous leukemia HL60 cells and normal human leukocytes. 2. Characterization of E-selectin binding fractions, and structural requirements for physiological binding to E-selectin. Biochemistry 35, 770-778 (1996) These are a few references that should get you going. Another reference that might be interesting for you would be: (1) Kohler, M.; Leary, J. A. (1995), Analytical Chemistry 673501-3508. LC/MS/MS of carbohydrates with postcolumn addition of metal chlorides using a triaxial electrospray probe. although I have not tried this myself. I will crosspost my answer together with your request to sci.techniques.mass-spec, you might get more replies from there. Good luck, Achim ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr. Achim Treumann Tel. 81-562-93-9381 Division of Biopolymer Science Fax 81-562-93-8832 Inst. Comprehensive Med. Sci. Fujita Health University Toyoake, Aichi 470-11, Japan ****************************************************************************** From: "Heinrich Luftmann" Date: Fri, 18 Apr 1997 08:51:00 -0700 Subject: Re: GC/MS: Mass es 91, 106 Organization: Org.Chem.Inst. Uni. Muenster David Shuster wrote: } } [This is forwarded from sci.chem.analytical - DB] } } I have a vacuum line inlet system attached to a gc/ms via a six port gas } sampling valve. When I run blanks onto a phaseless column from my vacuum } line, I see a small ethyl-benzene peak in my background. Does anyone have } any ideas where this contaminant would be coming from? Baking out my line } at 120 C does not appear to get rid of the contaminant. } } David Shuster } shuster@socrates.berkeley.edu You experimetal setup is not quit clear to me, but if you have a forepump connected to your vacuum inlet line there is often the problem of backstreaming from the pump. This is especially dangerous when you have only a low gasflow to the pump. A filter with active charcoal may help. I had a similar problem with a reference inlet system. Hope this will help Heinrich Luftmann luftman@uni-muenster.de ****************************************************************************** From: fred.mellon@bbsrc.ac.uk (FRP242) Date: 18 Apr 1997 08:46:30 GMT Subject: Re: Electrospray contaminants Organization: IFR NL In article <5j2kmv$h0p@acmey.gatech.edu>, Drew Gibson says: } }If anyone /does/ have such a beast, could they post to the newsgroup }rather than email the poster, as I'm sure a number of us are interested }in this. } }Do you have a particular problem Fred, or just out of interest ? } }Regards from an ex- UEA MSc spectroscopic and physical methods student }:) }-- } }Cheers, } } Drew ;^) } } }}} drew@thegibsons.demon.co.uk <<< } } No particular problem; every analysis seems to generate a different one! However, my experience is that contamination with polymeric material does seem to recur quite frequently, especially in negative ion ES. Regards, Fred Fred A Mellon Institute of Food Research Norwich Laboratory Norwich Research Park Colney Norwich NR4 7UA UK tel. +44 (0)1603 255299; fax +44 (0)1603 452578 ****************************************************************************** From: "Steven P. Cepa 847-937-7539" Date: Fri, 18 Apr 1997 10:37:00 -0500 (CDT) Subject: MCM-MSDG meeting Organization: * For the electronic crowd MCM-MSDG Presents "New Strategies in (bio) Pharmaceutical Analysis" Mark A. McDowall, Micromass UK Limited, Manchester, UK Date: Thursday, May 1, 1997 Venue: Cafe La Cave, 2777 S. Mannheim Road, Des Plaines, IL. Restaurant Phone: 847-827-7818 (not registration phone) Social hour starts at 5:30 pm, dinner at 6:30 pm. Dinner: Breast of Chicken Combo with soup, salad, vegetables and dessert. Registration and Dinner: $25.00 Reservations call Nancy Robinson (708-383-0707) on or before Monday, April 28, 1997. Directions: From the 294 Tri-State Tollway or the Northwest Tollway (90), exit at O'Hare (Rt. 190). Go west, then exit at Mannheim Road NORTH. Continue north on Mannheim about 1.5 miles. Restaurant is about two blocks north of Higgins, (Rt. 72) on east side of street. ABSTRACT The practice of mass spectrometry is being transformed - driven by the evolving requirements of the pharmaceutical industry. A new generation of "walk-up" API LC-MS systems is revolutionizing working practices across the industry. Classical centralized MS facilities, conceived in a pre-combinatorial era, are evolving into "MS servers" supporting distributed networks of "MS Clients" - dedicated high throughput workstations sited at the point of analytical need ... * Classical synthetic chemistry * Medicinal chemistry * Combinatorial chemistry / parallel synthesis * Screening * Bioavailability / Kinetics * Metabolism New orthogonal acceleration Time-of-Flight (oaTOF) and hybrid quadrupole - oaTOF analyzer technologies have emerged to address new challenges such as high throughput automated accurate mass measurement of combi-chem products, de novo sequencing of proteins at high sensitivity, and proteome elucidation The rate determining step analysis is not data acquisition, but rather the speed with which they can make use of the data they already have - the barriers to communication between data producers and consumers. More and more laboratories are adopting common platforms to facilitate virtual integration. Windows NT and HTML will be vitally important in this process - providing a de-facto standard leading to a true data - information continuum. Many firms such as Kodak and Glaxo already are making physical data available to their scientists over secure intranet systems. The presentation is intended to survey these developments and provoke debate. Mark A McDowall is international director of Micromass... the mass spectrometry people ****************************************************************************** From: Dejan Djokovic Date: Sat, 19 Apr 1997 15:07:40 +0200 Subject: FT Book & MAT 8200 High Voltage lost Organization: * Several questions and answers for List members! Recently (end of 1996) there was published new book on subject Fourier transform in spectroscopy/spectrometry including chapter on FT-MS. Unfortunatly I have lost reference. Does anybody have it? Concerning question on Finnigan MAT 8200 High Voltage lost. The Instrument has several steps protection of shortcirkuts system. So when this does happen it is nesescery to turn off entire ion source and then on for High Voltage to appear again. That is some relay switch. Also there is mechanical switch at the end of rack, if you do not put electronic rack back in it's position HV wont be back! If the problem persists then most probably it shall be nacessary to replace entire power suply transformer on BIC Unit. We had a similar problem. Best Regards, Mr Dejan Djokovic Phone.: +381-11-637-075 Faculty of Chemistry +381-11-630-474 University of Belgrade +381-11-328-2111 ext. 649 Studentski trg 16 P.O. Box 158 Fax: +381-11-636-061 11001-YU Belgrade Yugoslavia (Serbia) Mass Spectrometry Lab Manager Email: dejandj@hf01.chem.bg.ac.yu WWW: http://afrodita.rcub.bg.ac.yu/bu/hf/eng.html ****************************************************************************** From: Joerg.w@t-online.de (Jörg Wöhrmann) Date: 20 Apr 1997 10:29:43 GMT Subject: SCIENCE and Chemical abstracts Organization: Telekom Online Internet Gateway Hello, i try to find some articles and informations about combinatorial chemistry. Is it possible to get some articles or publications from SCIENCE or chemical abstracts via Internet. Can you send me the adresses and informations how to find somethig about it? Thank you Regards Joerg ****************************************************************************** From: cw400@freenet.carleton.ca (VictorB) Date: Mon, 21 Apr 1997 06:15:00 GMT Subject: The National Tour Speaker Program. Organization: National Capital Freenet, Ottawa, Canada OTTAWA VALLEY SECTION 1997 SAS TOUR SPEAKER DINNER MEETING featuring Dr. H.M. `Skip' Kingston Department of Chemistry and Biochemistry and Environment Science and Management Duquesne University, Pittsburgh, PA ......presenting...... Microwave Enhanced Sample Preparation for Environmental and Analytical Analysis Applications Date & Time: Thursday, May 1, 1997. Cash bar (6:00-7:00)pm. Dinner served at 7:00 pm sharp. Presentation scheduled to commence between (8:45-9:00) pm. Location: The National Press Club, 165 Sparks Street and 150 Wellington Street, Second Floor room library 1 and 2, (Ottawa-Ontario-Canada). Please use Sparks Street entrance (corner of O'connor). Parking: After 6:00pm on Queen Street or adjacent streets. Also pay parking at the World exchange Plaza located between Queen and Albert Street. Menu: Chef Salad, grilled chicken, Rice Pilaff, Seasonal Vegetables, Cream of Mint Parfait, dessert, followed by Tea or Coffee. Rates: $10.00 Students, $12.00 Members, $15.00 Non-Members Payment will be collected at the event. This event is subsidised by the Ottawa section, SSC. ABSTRACT:The use of microwave enhanced sample preparation has become a standard technique in the analytical environmental laboratory because it has demonstrated many superior capabilities over other more conventional methods. Microwave technology is now embarking on a path to becoming standard laboratory apparatus. It is a next generation fundamental analytical instrumental system. The current state of the art will be discussed and the strengths and weaknesses of microwave sample preparation will be analyzed in general, and specifically for environmental applications. The newer tools that have emerged and the significant new capabilities that they provide will be evaluated and projected. With the use of these tools, we have the possibility of providing generic and reproducible quality assurance methods in environmental and industrial microwave sample preparation that are instrument independent. The future research directions that are needed to continue the maturation of the technology will be discussed. Methods of coordinating and standardizing instrument control and providing standard instrumental performance will also be presented. New approaches in scientific information exchange that permit state of the art solutions to sample preparation problems and a new forum for solving these problems will be provided and discussed (1,2). 1. Kingston, H.M. Skip, and Haswell, Steve, Microwave Enhanced Chemistry, American Chemical Society Professional Reference Book Series, ACS, Washington, D.C., 1997. 2. H.M. Kingston, P.J. Walter, and S.J. Chalk, World Wide Web site at URL:http://www.sampleprep.duq.edu "SamplePrep Web", Duquesne University, Pittsburgh, PA (1996). Reservation: NO later than Monday April 28th 1997. Contact Victor Benham (Tel/Fax: 684 -4936; cw400@freenet.carleton.ca, or Mike Hinds (Tel: 993-8975; fax: 991-1741; email: hinds@rcmint.ca ****************************************************************************** From: Jorge Carvalho Silva Date: Mon, 21 Apr 1997 15:38:19 +0000 Subject: 3rd Portuguese Mass Spectrometry Meeting Organization: FCT/UNL The 3rd Portuguese Mass Spectrometry Meeting will take place in Lisbon, Portugal, from the 1st to the 3rd September, 1997. This meeting is devoted to all aspects of Mass Spectrometry, from instruments and techniques to the analysis of solid, liquid and gaseous samples. The scientific program will consist of lectures given by invited speakers and oral and poster presentations of submitted contributions. The social program will include a reception, a conference dinner and a boat tour. More info at http://cefitec.df.fct.unl.pt//3pmsm.html Jorge Carvalho Silva http://cefitec.df.fct.unl.pt/jcsilva/jcsilva.html ****************************************************************************** From: "Pneutronics" Date: 21 Apr 1997 14:51:04 GMT Subject: MS Companies Organization: Parker I am currently doing research on Mass Spec Companies. I am looking for a list of all companies that make a Mass Spec product. Any Info would be appreciated. Jill [Moderator's note: There is a Web page for STMS at . We have are links to many companies there, as well as links to other MS pages. DB] ****************************************************************************** From: Christine Van Roosen Date: Mon, 21 Apr 1997 11:37:11 -0400 Subject: Reasonably priced, powerful math software Organization: Macsyma Inc. Contacts: http://www.macsyma.com/ info@macsyma.com Macsyma Inc. has announced four new variants of PC Macsyma® 2.2 math software at introductory pricing (listed below). Math software reviewers agree that Macsyma is the easiest to use of the powerful math packages. Macsyma scores highest on the largest math test suites and costs less than half as much as competitive software. Contact Macsyma Inc. for a free working demo CD, academic or volume prices, or other information. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Special Price Regular Price PC Macsyma Lite $129 --- (limited capabilities, limited manuals) PC Macsyma 2.2 (limited manuals) $324 --- PC Macsyma 2.2 (full manuals) $399 $699 PC Macsyma 2.2 Pro (NumKit(tm), full manuals) $599 $769 UNIX Macsyma $999 $1,249 (offers PC notebook interface in "client-server mode.") ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NumKit is an accelerator for numerical linear algebra based on the Lapack library from Netlib. - Limited documentation includes the Macsyma Quick Reference Card, introductory documentation to get you started, and the installation guide and release notes. Full documentation sets also include 1,000 pages of bound documentation. Offer good from April 15 through September 30, 1997. Contacts: http://www.macsyma.com/ info@macsyma.com ****************************************************************************** From: "Peter Jacobs" Date: Sun, 20 Apr 1997 14:48:04 +0100 Subject: Re: Electrospray contaminants Organization: * }In article <5j2kmv$h0p@acmey.gatech.edu>, Drew Gibson } says: }If anyone /does/ have such a beast, could they post to the newsgroup }rather than email the poster, as I'm sure a number of us are interested }in this. A while ago PE Sciex produced a note entitled "A study on the effect of Biological Detergents on the Ion Evaporation Mass Spectra of Proteins" containing spectra of myoglobin mixed with a.o. SDS, Triton, Chaps, Brij 35 and Nonidet P-40. I hope this helps you (or someone else). Peter Jacobs N.V. Organon (AKZO Nobel) Dept. of Analytical Chemistry for Research Mass Spectrometry (Room RK 1221) P.O. Box 20, 5340 BH Oss The Netherlands E-mail: p.jacobs@organon.akzonobel.nl ****************************************************************************** From: fred.mellon@bbsrc.ac.uk (Fred Mellon) Date: 21 Apr 1997 16:03:15 GMT Subject: British Mass Spectrometry Society Web Site Organization: IFR NL The British Mass Spectrometry Society now has a web site at . This may be non-operational between 9 and 12:30 (GMT) on 22nd April because of network maintenance. Fred A Mellon Institute of Food Research Norwich Laboratory Norwich Research Park Colney Norwich NR4 7UA UK te. +44 (0)1603 255299; fax +44 (0)1603 452578 ****************************************************************************** From: gerry@cismibm.univ-lyon1.fr (Gerry Niccolai) Date: 22 Apr 1997 08:31:41 GMT Subject: Mass spec simulation : Source code Organization: Laboratoire COMS Hi, I would like the source code or a calculation algorithm for the calculation of isotope patterns for a give formula. I have the source code for the mass.exe and mass_win.exe programs, but they are in C and I would like the code in FORTRAN or BASIC (what a fossil!). What would really be great would be a spreadsheet worksheet that does the calculation for me, but I am probably dreaming, no? gerry ****************************************************************************** From: Asha Subject: Mass Spec Help! Date: Tue, 22 Apr 1997 02:38:59 -0600 Organization: SaskNet News Distribution Anyone know where I can get some reliable mass analysis done, for a reasonable price? Would like if they could do FAB, and have capabilities for LC-MS as well. Thanks for any info! ****************************************************************************** From: jonesy@chemiris.labs.BrockU.CA (Tim Jones) Date: Tue, 22 Apr 1997 12:06:46 -0500 Subject: Chemstation Printing Problem Organization: * Hi everyone! We have just upgraded our old HP 5890/5970 GC/MS with the PC interface, Windows95 and MSChemstation software (G1034C, Vers.03.04). The print support is via a nearby HP Laserjet 5MP which is queued through one of our SGI servers. I have printed from a number of applications including a variety of text sources, graphics from Grams32 and Explorer, yet I can only succeed in getting text reports out of the Data Analysis module of MS Chemstation. When a chromatogram or spectrum is Printed, the program reports that the files have gone to the spooler, yet no output is generated! The program has almost no print configuration features since it uses the Windows drivers. Has anyone else experienced this problem? There is nothing showing up in the bug list on HP's web site, but I am waiting to hear back from the local support group. I hardly expected to have such a problem with an HP printer! Regards, Tim _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Tim Jones _/ Coordinator, Analytical Services _/ Dept. of Chemistry, _/ Brock University, _/ St. Catharines, Ontario _/ CANADA L2S 3A1 _/ Phone: 905-688-5550 Ext. 3850/4172 _/ FAX: 905-682-9020 _/ e-mail: jonesy@chemiris.labs.BrockU.ca _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ ****************************************************************************** From: Michael J. Haas Subject: instrument "for sale" Date: 21-Apr-1997 ****** INSTRUMENT FOR DONATION/SALE: FINNIGAN MAT 8230 Acquisition Date: spring 1986 (data system: spring 1993) Features: reverse-geometry sector instrument; two complete EI/CI sources w/FAB adaptations; Cs FAB gun; DEP and DIP probes. Complete w/turbomolecular and rotary vacuum pumps, plenty of spare source parts, o-rings, electronic bits, etc. Data system: Sun SPARC IPC Station with Kratos Mach3X operating system and software; full color monitor; laser printer. Condition: instrument is fully functional and has been used primarily for NH3-CI probe work on small organic/pharmaceutical compounds. Availability: mid- to late summer, contingent on our purchase of replacement instrument. * This instrument is being offered for donation. Prefer recipient to pay shipping costs but negotiable. * SPARC/Kratos data system is also *for sale* separately, if desired; all offers considered. Contact: Michael J. Haas The DuPont Merck Pharmaceutical Company DuPont Experimental Station P.O. Box 80500 Wilmington, DE 19880-0500 tel: (302) 695-7328 FAX: (302) 695-8943 email: haasmj@lldmpc.dnet.dupont.com ****** ****************************************************************************** From: dennis.benjamin@molecular-sciences.oxford.ac.uk (Dennis Benjamin) Date: 22 Apr 1997 19:01:10 GMT Subject: ANDI format Organization: Oxford University, England Hi all: Can anyone point me toward a description of the ANDI data format? Our vendor (Micromass) say that they can import data in this format, so I'd like to figure out how to convert data from our other machine into ANDI format. Thanks for any help, ****************************************************************************** From: vanreet@ilink.nis.za (Teunis vanRee) Date: 22 Apr 1997 19:31:39 GMT Subject: Re: SCIENCE and Chemical abstracts Organization: Network Information Services Jörg Wöhrmann (Joerg.w@t-online.de) wrote: : Hello, : i try to find some articles and informations about combinatorial : chemistry. Is it possible to get some articles or publications from : SCIENCE or chemical abstracts via Internet. : Can you send me the adresses and informations how to find somethig about : it? Use your favourite browser with keywords +combinatorial +chemistry, also search for work by Mark A Gallop. You might also find a literature list here if you can search backwards for one of my postings abt a month ago. Regards Thomas ****************************************************************************** From: "Hilton Evans" Date: 23 Apr 1997 20:07:21 GMT Subject: Drawing Chemical Structures Organization: The Internet Connection Chempen+ Chemical Structure Drawing Software for Windows http://www.ici.net/cust_pages/hfevans/chempen.htm ****************************************************************************** From: taylord@ecn.purdue.edu (David G Taylor) Date: 23 Apr 1997 22:31:16 GMT Subject: Re: Chemstation Printing Problem Organization: Purdue University, Chemical Engineering In article <5jipm1$p06@acmey.gatech.edu>, jonesy@chemiris.labs.BrockU.CA (Tim Jones) wrote: } Hi everyone! } } We have just upgraded our old HP 5890/5970 GC/MS with the } PC interface, Windows95 and MSChemstation software (G1034C, Vers.03.04). } The print support is via a nearby HP Laserjet 5MP which is queued through } one of our SGI servers. I have printed from a number of applications } including a variety of text sources, graphics from Grams32 and Explorer, } yet I can only succeed in getting text reports out of the Data Analysis } module of MS Chemstation. When a chromatogram or spectrum is Printed, the } program reports that the files have gone to the spooler, yet no output is } generated! The program has almost no print configuration features since } it uses the Windows drivers. Has anyone else experienced this problem? } There is nothing showing up in the bug list on HP's web site, but I am } waiting to hear back from the local support group. I hardly expected to } have such a problem with an HP printer! } } } Regards, I suspect the SGI box is adding a header or altering the header of the file that is spooled from your ChemStation, and that doesn't make the printer happy. Try to capture the file in the print spooling directory on the SGI box; you may need to "down" the printer for this to keep the file around long enough to copy to your local directory. Then on the PC, print the same job to a file, keeping the same printer type.. Compare the files, and I think you'll find the problem. When you look at the files, be aware you need to be able to see control characters, like ctrl-D, ctrl-L, etc.. If memory servers correctly, PostScript printers want: %!PS-Adobe-1.0 as the very first line, no blank lines ahead, no CRs, LFs, etc.. Dr. David G. Taylor - Director - Instrumentation Purdue University, School of Chemical Engineering, 1283 CHME Blvd. West Lafayette, IN 47907-1283 USA V(765) 494-4085 Fax(765) 494-0805 -- taylord@ecn.purdue.edu ****************************************************************************** From: mhugley@smash.gatech.edu (Captain Mark Hugley) Date: Thu, 24 Apr 1997 03:35:54 GMT Subject: Re: Chemstation Printing Problem Organization: South Carolina Law Enforcement Division jonesy@chemiris.labs.BrockU.CA (Tim Jones) wrote: }one of our SGI servers. I have printed from a number of applications }including a variety of text sources, graphics from Grams32 and Explorer, }yet I can only succeed in getting text reports out of the Data Analysis }module of MS Chemstation. When a chromatogram or spectrum is Printed, the }program reports that the files have gone to the spooler, yet no output is }generated! The program has almost no print configuration features since }it uses the Windows drivers. Has anyone else experienced this problem? }There is nothing showing up in the bug list on HP's web site, but I am }waiting to hear back from the local support group. I hardly expected to }have such a problem with an HP printer! The ChemStation spooler is limited to eight character name. We had to reduce the name of our PC to eight characters and reduce the name of the Network Printer to less than eight characters before this worked correctly. ****************************************************************************** From: Chris Moore Date: Thu, 24 Apr 1997 07:14:14 GMT Subject: Re: SCIENCE and Chemical abstracts Organization: Queensland Department of Primary Industries Teunis vanRee wrote: } } Jvrg Wvhrmann (Joerg.w@t-online.de) wrote: } : Hello, } } : i try to find some articles and informations about combinatorial } : chemistry. Is it possible to get some articles or publications from } : SCIENCE or chemical abstracts via Internet. } } : Can you send me the adresses and informations how to find somethig about } : it? } } Use your favourite browser with keywords +combinatorial +chemistry, also } search for work by Mark A Gallop. You might also find a literature list } here if you can search backwards for one of my postings abt a month ago. } } Regards } } Thomas I sent the following reply directly to Jorg some time ago. The info. may be of general interest, however: Check out http://pubs.acs.org/hotartcl/cenear/970224/comb.html Cheers, Chris Moore ****************************************************************************** From: jnegley@uhl.uiowa.edu (John Negley) Date: 24 Apr 1997 20:02:10 GMT Subject: Spectral data web site for compounds? Organization: The University of Iowa I was wondering if there is a web site for spectral data on different compounds. We are using a NIST library but it doesn't have the spectral data for a couple of compounds which we have the CAS numbers for. Thanks for the help. ****************************************************************************** From: Thierry Mann Subject: HP 5970 MS Date: Thu, 24 Apr 1997 16:45:08 -0700 Organization: University of Arizona [This is forwarded from sci.chem.analytical - DB] Hi folks, we just got a surplus HP 5970 Mass spec. We are missing however the "analyzer top cover" that feeds all the wires into the boards inside. Anybody out there got a scrap 5970 that we could trade for or come to some agreement? We were also curious about modifying the computer interface to make a 5970 run through a 486 PC. Has anyone modified a 5970 to run on windows based software? There is an artical in J chem Ed about modifying a 5985 mass spec but I have never seen one of these. Thanks in advance for any info and help. please respond to tmann@theriver.com ****************************************************************************** From: Mike Wright Subject: Re: HP 5970 MS Date: Fri, 25 Apr 1997 13:57:10 +0100 Organization: Health and Safety Lab [This is forwarded from sci.chem.analytical - DB] In article <335FF084.6830@theriver.com>, Thierry Mann writes }Hi folks, we just got a surplus HP 5970 Mass spec. We are missing }however the "analyzer top cover" that feeds all the wires into the }boards inside. Anybody out there got a scrap 5970 that we could trade }for or come to some agreement? } }We were also curious about modifying the computer interface to make a }5970 run through a 486 PC. Has anyone modified a 5970 to run on windows }based software? There is an artical in J chem Ed about modifying a 5985 }mass spec but I have never seen one of these. } }Thanks in advance for any info and help. }please respond to tmann@theriver.com In 1995 we upgraded a 5970 MSD we bought in 1989 from the Unix O/S to MS Windows. We run the Chemstation software rev B.02.05 with no problems on a 486/66 + 16Mb RAM. As far as I know you will not have to change any interface on the 5970, although 5970 hardware can be much older than ours -back to about 1981 I think. What does your local HP office say? -- Mike Wright, HSL Sheffield UK ****************************************************************************** From: mchem1@uconnvm.uconn.edu Date: 25 Apr 1997 14:28:20 GMT Subject: Re: Chemstation Printing Problem Organization: University of Connecticut On 1997-04-22 jonesy@chemiris.labs.BrockU.CA(TimJones) said: }Hi everyone! }We have just upgraded our old HP 5890/5970 GC/MS with the }PC interface, Windows95 and MSChemstation software (G1034C, Vers.03. }04). }}snip<< }Regards, }Tim }_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ }Tim Jones _/ }Coordinator, Analytical Services _/ }Dept. of Chemistry, _/ }Brock University, _/ }St. Catharines, Ontario _/ }CANADA L2S 3A1 _/ }Phone: 905-688-5550 Ext. 3850/4172 _/ }FAX: 905-682-9020 _/ }e-mail: jonesy@chemiris.labs.BrockU.ca _/ }_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Tim: I had the same problem with an LC ChemStation I upgraded from Windows for Workgroups to W95. HP's answer: Not Compatible. It behaved the same way you describe, no graphics printout. I reloaded WFW in a separate directory so I could boot into either OpSys and that fixed the problem. Regards, Al Kind, Tech Lab Mgr: Microchem Lab U-193,Univ of CT Storrs CT 06269-4193, Phone / FAX (860)486-6126 / 6124 Net-Tamer V 1.08 Palm Top - Registered ****************************************************************************** From: li@pine.circa.ufl.edu Date: 25 Apr 1997 22:31:28 GMT Subject: Help: Ph.D. Analytical Chemist/Spectroscopist Organization: University of Florida - CIRCA Dear Collegues, I am a Ph.D. analytical chemist/spectroscopist working as a postdoc at Battelle-Pacific Northwest National Laboratory. As my appointment is getting to an end, I am looking for an industrial or teaching position. I have a total of eight years experience with a variety of analytical techniques, including spectroscopy (IR,Raman,UV-VIS,UPS), mass spectrometry (LD-TOF MS,MALDI, Electrospray), thermal analysis (TGA,TGA-FTIR,DSC), and chromatography (GC,GC-MS,GC-FTIR,HPLC,LC-MS). If you have any information regarding employment opportunities with my background, I would very much appreciate to hear from you. Thanks in advance. Sam Li Pacific Northwest National Laboratory Richland, WA s_li@pnl.gov 509-372-4505 ****************************************************************************** From: "James Duckworth" Date: 26 Apr 1997 13:31:46 GMT Subject: Re: Spectral data web site for compounds? Organization: AT&T WorldNet Services John Negley wrote in article <5jos3e$ftg@acmey.gatech.edu>... } I was wondering if there is a web site for spectral data on different } compounds. We are using a NIST library but it doesn't have the spectral } data } for a couple of compounds which we have the CAS numbers for. Thanks for } the help. My company, Galactic Industries, has a bunch of spectral data posted on our web site (http://www.galactic.com). Right now it's mostly FT-IR and other optical spectra, but we'll be posting more in the very near future, including some MS data. Most of this data is stuff we've found available for free on the Internet or donated to us for posting on the Web site. We just reformatted it into the SPC file format used by our software products. There's also a free viewer program you can download for looking at the SPC files. Our collection is not too complete right now, so if you search on the CAS numbers and don't find anything, you may want to check back later. Also, much of the data didn't come with CAS numbers and we have not yet taken the time to go back and assign them to the ~5000 spectra that don't have them yet. In due time... I hope this helps. -- James Duckworth Galactic Industries Corp. 395 Main Street Salem, NH 03079 Voice: (603) 898-7600 Fax: (603) 898-6228 E-mail: jhd@galactic.com ****************************************************************************** From: chrisjcr@aol.com (ChrisJCR) Date: 26 Apr 1997 16:29:00 GMT Subject: Re: Chemstation Printing Problem Organization: AOL http://www.aol.com In article <5jipm1$p06@acmey.gatech.edu>, jonesy@chemiris.labs.BrockU.CA (Tim Jones) wrote: } Hi everyone! } } We have just upgraded our old HP 5890/5970 GC/MS with the } PC interface, Windows95 and MSChemstation software (G1034C, Vers.03.04). } The print support is via a nearby HP Laserjet 5MP which is queued through } one of our SGI servers. I have printed from a number of applications } including a variety of text sources, graphics from Grams32 and Explorer, } yet I can only succeed in getting text reports out of the Data Analysis } module of MS Chemstation. When a chromatogram or spectrum is Printed, the } program reports that the files have gone to the spooler, yet no output is } generated! The program has almost no print configuration features since } it uses the Windows drivers. Has anyone else experienced this problem? } There is nothing showing up in the bug list on HP's web site, but I am } waiting to hear back from the local support group. I hardly expected to } have such a problem with an HP printer! } } } Regards, Tim, Just a couple of suggestions - If your printer is a network printer, the ChemStation software doesn't like to print to a network address such as "\\networkpc\hpdeskjet..." It typically requires that the network printer be captured to a printer port such as "LPT2: (\\networkpc\hpdeskjet)". This can be changed in the printer "properties" dialogue box under the "details" tab. You must first capture the port, which results in assigning the network printer path to an LPT port. Then tell the driver to print to that port (instead of the network address). If this isn't the source of the problem, try printing to the LaserJet 5MP using the LaserJet III or 4 drivers. To do this, simply add a printer (laserjet III) and direct ouput to your LaserJet 5. The drivers should be available on your Win95 CD. (I'm assuming you are not in PostScript mode) Chris Richardson Cryovac, W.R. Grace ChrisJCR@aol.com http://members.aol.com/chrisjcr ****************************************************************************** From: cw400@freenet.carleton.ca (VictorB) Date: Sun, 27 Apr 1997 00:54:13 GMT Subject: The 1997 National Tour Speaker Program Organization: National Capital Freenet, Ottawa, Canada OTTAWA VALLEY SECTION 1997 SAS TOUR SPEAKER DINNER MEETING featuring....... Dr. H.M `Skip' Kingston Department of Chemistry and Biochemistry and Environment Science and Management Duquesne University, Pittsburgh, PA ......presenting...... Microwave Enhanced Sample Preparation for Environmental and Analytical Analysis Applications. Date & Time: Thursday, May 1, 1997. Cash bar (6:00-7:00)pm. Dinner served at 7:00 pm sharp. Presentation scheduled to commence between (8:45-9:00) pm. Location: The National Press Club, 165 Sparks Street and 150 Wellington Street, Second Floor room library 1 and 2 (Ottawa-Ontario-Canada). Please use Sparks Street entrance (corner of O'connor). Parking: After 6:00pm on Queen Street or adjacent streets. Also pay parking at the World exchange Plaza located between Queen and Albert Street. Menu: Chef Salad, grilled chicken, Rice Pilaff, Seasonal Vegetables, Cream of Mint Parfait, dessert, followed by Tea or Coffee. Rates: $10.00 Students, $12.00 Members, $15.00 Non-Members Payment will be collected at the event. This event is subsidised by the Ottawa section, SSC. ABSTRACT:The use of microwave enhanced sample preparation has become a standard technique in the analytical environmental laboratory because it has demonstrated many superior capabilities over other more conventional methods. Microwave technology is now embarking on a path to becoming standard laboratory apparatus. It is a next generation fundamental analytical instrumental system. The current state of the art will be discussed and the strengths and weaknesses of microwave sample preparation will be analyzed in general, and specifically for environmental applications. The newer tools that have emerged and the significant new capabilities that they provide will be evaluated and projected. With the use of these tools, we have the possibility of providing generic and reproducible quality assurance methods in environmental and industrial microwave sample preparation that are instrument independent. The future research directions that are needed to continue the maturation of the technology will be discussed. Methods of coordinating and standardizing instrument control and providing standard instrumental performance will also be presented. New approaches in scientific information exchange that permit state of the art solutions to sample preparation problems and a new forum for solving these problems will be provided and discussed (1,2). 1. Kingston, H.M. Skip, and Haswell, Steve, Microwave Enhanced Chemistry, American Chemical Society Professional Reference Book Series, ACS, Washington, D.C., 1997. 2. H.M. Kingston, P.J. Walter, and S.J. Chalk, World Wide Web site at URL:http://www.sampleprep.duq.edu "SamplePrep Web", Duquesne University, Pittsburgh, PA (1996). Reservation: NO later than Wednesday April 30th 1997. Contact Victor Benham (Tel/Fax: 819-684 -4936; cw400@freenet.carleton.ca, or Mike Hinds (Tel: 613-993-8975; fax: 613-991-1741; email: hinds@rcmint.ca ****************************************************************************** From: Michael Jordan Date: Mon, 28 Apr 1997 08:01:03 -0400 Subject: Derivatizing hydroperoxy fatty acids? Organization: * We are investigating hydroperoxy fatty acids (chain C14-C20). Due to the nature of the hydroperoxy group GC analysis will require derivatization of both the hydroperoxy and carboxylic acid groups. Has anyone knowledge of a procedure to stabilize the hydroperoxy group but still allow alkylation at the carboxylic acid (eg. MeOH-BF3)? We have thought of reducing with boron tetrahydride and then silating. Any thoughts regarding this? Final analysis will utilize EI-MS. Please reply directly to: Jordanm@em.agr.ca Thank you in advance. Michael Jordan GC-MS Technician Agriculture & Agri-Food Canada 32 Main Street Kentville, NS B4N 1J5 CANADA Tel: 902-679-5528 Fax: 902-679-2311 E-mail: jordanm@em.agr.ca ****************************************************************************** From: Keith Martinko Date: Mon, 28 Apr 1997 14:08:15 -0500 Subject: Re: HP 5970 MS Organization: ProLab Resources, Inc. Dear Thierry, ProLab Resources has a PC-based data system replacement for your HP 5970 MS that allows you to replace either your current HP Pascal or RTE data system with easy-to-use, multi-tasking acquisition and processing software. For more information, visit our WWW site at http://www.prolab.com. Also, Dr. Vicki Wysocki at the University of Arizona is currently using a number of our data systems on some of her MS equipment. Please give contact her if you are interested in seeing a "live" demonstration of the system. Sincerely, Keith Martinko ProLab Resources, Inc. http://www.prolab.com prolab@ix.netcom.com ****************************************************************************** From: "Steven P. Cepa 847-937-7539" Date: Tue, 29 Apr 1997 13:09:00 -0500 (CDT) Subject: Happy Anniversary Organization: * It was on April 30, 1897 that J.J. Thomson gave his announcement of the discovery of the "corpuscle" (aka, the electron) at a Friday Evening Discourse at the British Royal Institution. His painstaking measurements (26 separate experiments) showed that these corpuscles were negatively charged particles; were all the same mass; and "that the mass of these particles is less than one-thousandth part of the mass of an atom of hydrogen". Thomson was awarded the Nobel Prize in 1906 for this work. He extended the "corpuscle" work to positive rays. It culminated in Thomson's 1913 book, "Positive Rays and Their Application to Chemical Analysis" with the concluding words: The positive rays thus seem to promise to furnish a method of investigating the structure of the molecule, a subject certainly of no less importance than that of the atom. This study has become the science of mass spectrometry. For your enjoyment, read the history of this event in RCMS 11 p2-16 (1997). ****************************************************************************** From: rathbun@sedona.net (Rathbun, Sapir & Associates) Date: Tue, 29 Apr 1997 17:49:08 GMT Subject: Position: Analytical Chemistry - Methods Development Organization: Sedona Internet Services, Inc. Analytical Assay Development Scientist We are seeking a scientist experienced in the development, optimization and validation of analytical chromatography assays to support our biopharmaceutical client company’s recombinant products. GENERAL JOB RESPONSIBILITIES: Develop HPLC, GC, & electrophoretic assays such as SDS-PAGE, western blot, IEF, CE, amino acid analysis, and monosaccharide analysis. Analytical methods development, optimization, & validation to support Phase I, II, and III clinical products (monoclonal antibodies and subunit vaccines). Assays pertain to both product characterization and measurement of trace levels of process contaminants. Supervise 6 bench scientists (levels B.S., M.S., and Ph.D.). Provide significant intellectual input to methods development; critically review all analytical results. Have solid experience in working in a GMP/GCP laboratory. Write SOPs, validation protocols and reports, and analytical sections of IND and BLA submissions. DESIRED PROFILE: Ph.D. in a biological or chemical science or MS-level candidate with a strong track record; preferably analytical chemistry, biochemistry, chemistry, chemical engineering. 4 or more years of pharmaceutical/biotechnology work experience company. Supervisory experience (2 or more employees). If you have an interest in this or other opportunities, please send us your resume/CV as an attached file to an email or by mail/FAX to RS&A to the attention of Ann G. Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520)284-3361 FAX E-mail: rathbun@ sedona.net ****************************************************************************** From: Bill Date: Wed, 30 Apr 1997 00:31:40 -0400 Subject: FD emitters Organization: The Netside Network Does anyone out there know if there is a company which sells FD emitters? (Does anyone still do FD??) Please respond by email to cotham@psc.sc.edu Thanks in advance Bill ****************************************************************************** From: mrdio007@aol.com (MrDio007) Date: 30 Apr 1997 23:45:59 GMT Subject: Re: LCQ vacuum problems Organization: AOL http://www.aol.com are you regularly balasting the pumps? matt sweeney ****************************************************************************** From: tedd@netcom.com (Ted Johnson) Date: Thu, 1 May 1997 03:26:41 GMT Subject: Web sites of Interest to Toxicologists Organization: Netcom On-Line Services I've was assigned a workshop for the SOFT/CAT meeting in Las Vegas this Nov. The topic is "The Internet and it's Resources for Toxicologists (for free)". I'm looking to make a complete list of as many resources available on the net as possible. This would include mass spec, UV, FTIR spectra, MSDS sources, net publications and a host of other topics. If anyone has sites that are helpful or interesting please let me know. I'll be glad to give you the list when it is complete. Thanks much. -- ******************************************************** Sincerely, Ted Johnson, President, Hair Trac Technology, Inc. tedd@netcom.com http://www.htrac.com ******************************************************** ****************************************************************************** From: "Little, James" Date: Thu, 1 May 1997 12:33:03 -0400 Subject: Web Page Organization: * I have created a Web page including the information on the following topics: -Creating HP user libraries with structures -Creating Combined HP libraries to overcome inefficient and ineffective library searching on HP systems -Construction of a Versatile Chemical Ionization Manifold -Methylamine/methane CI for minimizing fragmentation in labile molecules -By-products (Artifacts) in the silylation of organic compounds see http://www.preferred.com/~s_little/mspec/index.html James Little Eastman Chemical Company Kingsport, TN 37662 423-229-8685 ****************************************************************************** From: flderoos@mmm.com Date: Thu, 01 May 97 15:39:55 -0600 Subject: Minnesota Mass Spectrometry Discussion Group Speakers for May Organization: * The Minnesota Mass Spectrometry Discussion Group will be hosting the following speakers in May. May 8, 1997 University of Minnesota Food Science Building, Room 15 St. Paul Campus 5:30 - 7:00 pm APPLICATIONS of TOF-SIMS FOR THE CHARACTERIZATION OF ORGANIC AND POLYMER SURFACES Dr. Anna Belu, Physical Electronics Company "A dramatic growth in the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) to organic and polymer systems has recently occurred, including the characterization of the surface and interfacial properties that influence important technological parameters such as adhesion, wettability, and biocompatibility. This seminar will highlight recent research in the use of TOF-SIMS in organic applications for the elucidation of bulk polymer properties and surface chemical information. The studies provide the basis for developing and evaluating the capabilities of TOF-SIMS, and for comparing them with complimentary analytical techniques. Specific examples will include the use of TOF-SIMS to characterize mixed self-assembled monolayer systems and to quantify endgroup functionalized polymers." Pizza and refreshments served at 5:30 pm, talk begins at 6:00 pm. May 22, 1997 Minnesota Chromatography Forum 18th Annual Spring Symposium Minneapolis Convention Center, Room 208A 10:30 am GC/MS AND LC/MS DETERMINATION OF LSD AND ITS METABOLITES IN PHYSIOLOGICAL SPECIMENS Dr. Rodger L. Foltz, University of Utah-Salt Lake City, Department of Pharmacology and Toxicology "Continued illicit use of the potent psychedelic drug LSD has stimulated efforts to develop effective analytical methods for detection of the drug and its metabolites in body fluids from suspected LSD users. Recently reported methods based on gas and liquid chromatography, combined with single- and multiple-stage mass spectral analysis, now permit accurate detection and quantitation of LSD at sub-ng/mL concentrations. However, concentrations of LSD in blood and urine often fall below current detection limits within one day following ingestion of the drug. Further research is directed at identifying LSD metabolites that can be detected over a longer time period." For additional information about of these presentations, please contact either: Tom Krick at tkrick@biosci.cbs.umn.edu or Fred L. DeRoos at flderoos@mmm.com or by telephone at 612-736-0665 Fred L. DeRoos, Ph.D. Senior Research Specialist 3M Corporate Research Laboratories Maplewood, MN "Opinions expressed herein are my own and do not necessarily represent those of 3M." ****************************************************************************** From: "Lee, Ji Hyun" Date: Fri, 17 Jan 1997 21:58:45 +0900 Subject: Methods to identify homodimer or homotrimer? Organization: College of Pharmacy, Seoul National University Are there any methods identifying whether a protein is a homodimer or a homotrimer? I have a small DNA-binding protein(6,100 dalton) quite well purified. Through size exclusion HPLC gel chromatography, I found its effective molecular weight is about 19,000 dalton, close to homotrimer. But I am not sure that it is a trimer based on only the result becuase I didn't ever heard about homotrimer DNA binding protein; so I need some additional experiments confirming this fact. What methods are adequate to this case? MADI-TOF analysis and measure of correlation time by NMR spectroscopy are under consideration, although I wonder they are appropriate. Thank you. ****************************************************************************** From: "F.A. Pinkse" Date: Fri, 02 May 1997 09:03:09 +0200 Subject: Re: FD emitters Organization: Academic Computer Services Amsterdam (SARA) Bill wrote: } } Does anyone out there know if there is a company which sells FD } emitters? (Does anyone still do FD??) Please respond by email to } cotham@psc.sc.edu } } Thanks in advance } } Bill Dear Bill Linden-ChroMasSpec Dulonweg 2 P.O. Box 610307 D-2800 Bremen 61 Germany Tel: (0421)821202 This company made FD emitters and FD Ion sources. The address and telephone number may be old. Hope to have helped you to go the right track. Frans ****************************************************************************** From: "Thomas Mattisson" Date: 2 May 1997 07:20:45 GMT Subject: Weight in different liquids? Organization: BIO-VÄX I have a disagreement with a friend about weights in liquids. The weight of a pice of iron is 1 kg, what is the weight in water? And in wich liquid will the weight be as least as possible? Please help us to sort out the problem Best regards Thomas ****************************************************************************** From: "Richard G." Date: Fri, 02 May 1997 11:57:30 +0100 Subject: Low temperature spectroscopy Organization: * Dear Spectroscopist I am very interested in low temperature analytical techniques, my field of interest is specifically x-ray diffraction. I was wondering if anyone could provide me with information on NMR, IR and ESR nitrogen sample cooling systems / cryostats or point me in the direction of some useful web sites. What is commercially available? Who are the manufacturers? How successful is this technique and how is the data improved, is it simply peak shape? What are the temperature ranges and stability requirements? What are the issues involved when selecting a cooler with an NMR, IR or ESR spectrometer? Please reply to richard@cgraph.demon.co.uk. Thank you in advance. Richard ****************************************************************************** From: rxw13@cwru.edu (Dr. Ron) Date: Fri, 2 May 1997 12:09:48 E.S.T. Subject: Re: Derivatizing hydroperoxy fatty acids? Organization: Case Western Reserve University, Cleveland OH (USA) Michael Jordan writes: }We are investigating hydroperoxy fatty acids (chain C14-C20). Due to the }nature of the hydroperoxy group GC analysis will require derivatization of }both the hydroperoxy and carboxylic acid groups. }Has anyone knowledge of a procedure to stabilize the hydroperoxy group but }still allow alkylation at the carboxylic acid (eg. MeOH-BF3)? We have }thought of reducing with boron tetrahydride and then silating. Any }thoughts regarding this? I'm too lazy to look it up for you ... but there was an article by (I think) Gus Graff in Methods of Enzymology about 10-15 years ago on isolation and characterization of 15-HETE and 15-HPETE (or on 15-lipoxygenase). I'm not sure the HPETE was in fact derivatized, but rather analyses were done before and after reduction of the HPETE to a HETE -- any of the silylaton ragents work fine on the hydroxylradical ... usual proceure is to methylate first (dizomethane) then TMS. ****************************************************************************** From: "Gary J. Van Berkel" Date: 2 May 1997 22:27:57 GMT Subject: Request for advice on VG PlasmaQuad TRA software Organization: Lockheed Martin Energy Systems We would like to request any potentially helpful advice regarding the frequent failure-in-use of the Time Resolved Acquisition (TRA) software associated with the VG PlasmaQuad II+ unit for ICP-MS. Our software is Resource Version 4.1.2 (Revision Date 15 Dec. 1994) operating under OS 2 on a 486 MHz PC. We have recently been attempting to use the TRA software to monitor uranium isotopes in an isotope-dilution MS quantitation experiment for certified samples. Almost always, the run bombs anywhere from halfway to two-thirds of the way through the requested total time, kicking us completely out of the VG software. Obviously, it is necessary to go thrrough the whole re-entry to restart another TRA file prior to running the next sample; aside from the annoyance factor, this makes automation of any process requiring TRA unfeasible. We have tried going to shorter run times with a somewhat higher rate of success, but anything over 5 minutes seems to be very risky. In addition, data from successfully completed runs are often subject to random problems when examined; commands to zoom in or out can leave part of the displayed data file unchanged while other portions alter as requested, while requests to print out a file usually request in a horizontal section of the graph being printed out while top and bottom are lost. Of course, the major problem at present is the inability to set up TRA recording that has enough run time for sucessive replicate analyses. We have been in contact with a U.S. representative for VG, who gave a few suggestions (we'd already tried some of them without success) such as keeping all other programs closed during TRA operation. He also claimed that a new version of TRA is on the way - but no clue as to when. In the meantime, we are working around the software bomb-outs as best we can using very brief run times. It is our understanding that other users have run into problems with the various versions of TRA as well. If any readers have found similar problems and ways to solve them (or at least to reduce the frequency of bombing out), we would reatly appreciate information you have to give. Even information regarding individual VG personnel who could really give useful advice on the TRA (if you know of any) would help. Please contact me at prettyjr@ornl.gov, or my research mentor Gary Van Berkel at the e-mail address above. Our thanks in advance, and we look foward to hearing from you. Dr. Jack Pretty Chemical & Analytical Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831-6375 ****************************************************************************** From: michael andrews Date: Fri, 02 May 1997 20:13:05 -0700 Subject: ? Organization: Oklahoma State University, Stillwater OK Could anybody help me with a problem this evening? I need to find a place where i can check mass spec data for compounds. Also, it would be helpful if I could look at archived specs. Any help would be appreciated. Thanks in advance. Michael ****************************************************************************** From: "James Duckworth" Date: 4 May 1997 15:10:52 GMT Subject: Searchable Spectral Database on Web Organization: AT&T WorldNet Services Galactic Industries has just posted a searchable database of spectra on its Web site (http://www.galactic.com/galactic/_gala/data.htm). The database contains approximately 10000 spectra of various compounds and incudes FT-IR, 1H & 13C NMR, and MS spectra. This is a collection of "free" data we've found on the 'Net, FTP sites and collections of publicly available data we've had lying around for years. The collection is kind of random, but we're looking to expand it and continually add to the database. All the spectra are stored in the database in Galactic's SPC file format, and include the compound name. Some entries have other information like CAS number and molecular formula, but again this is not complete throughout the entire database. After searching the spectral data can be viewer directly in a browser, or you can down load the spectral data files and use our free spectral viewer (dowloadable from http://www.galactic.com/galactic/_gala/data.htm) to look at them. Please try it out and give us your comments. If there is enough interest, and people are willing to make contributions of data (only non-proprietary stuff please!) we are planning to continually exand the collection and add new searchable fields of information. Also, if anybody knows of any other "public" collections of data available o the 'Net, we'd love to know about that too. Doesn't matter what data format they're in, we can translate pretty much everything. Thanks in advance, and I hope this thing proves useful. BTW - don't respond to this message to contact me, use the address below. This is a home e-mail account I don't check very often. -- James Duckworth Galactic Industries Corp. 395 Main Street Salem, NH 03079 Voice: (603) 898-7600 Fax: (603) 898-6228 E-mail: jhd@galactic.com ****************************************************************************** From: PAREESDM@apci.com Date: Mon, 5 May 1997 12:20:30 -0400 Subject: Re: FD emitters Organization: * }Does anyone out there know if there is a company which sells FD }emitters? (Does anyone still do FD??) Please respond by email to }cotham@psc.sc.edu } }Thanks in advance} } }Bill] Someone already mentioned Linden. VG (Micromass in Beverly, MA used to also provide them, at least for their instruments. You didn't mention what brand of instrument you are using. GB Scientific, in Novato, CA also makes them from time to time. Yes, a few labs still use FD extensively, including, I believe, Upjohn. Also, you might want to contact Bob Lattimer (look him up in the ASMS directory). Jim Scrivens, ICI, England, also does FD. I do a bit myself. Dave Parees My answers are not backed by the full faith and value of my company ****************************************************************************** From: Ivan Date: Tue, 06 May 1997 01:45:44 +0800 Subject: HELP!!! Organization: Netvigator Anyone has experience in using Finnigan MAT 95S mass spectrometer, I am wondering if it is a tandem one, and the type of ionization it is capable. Thanks for your help. -- mailto:ivanyip@netvigator.com or mailto:ivanyip@usa.net ****************************************************************************** From: A.Dietrich@em.uni-frankfurt.de (Armin Dietrich) Date: Mon, 05 May 1997 21:37:27 GMT Subject: Injector leak? GC3400 / ITS 40 Organization: J. W. Goethe-Universitaet Frankfurt/Main Can anyone help me? I have a problem with a "leak" in the injector system of a Varian GC 400, connected to a Finnigan MAT ITS 40. If I set the flow of the injector split and septum purge to "normal" flow rates (30 ml He split / 5 ml He purge), the MS indicates a leak system. (Ratio N2/H20/CO2 o.k, according to the manual) Increasing the septum purge to 60 ml, the system seems to be tight. (Ratio N2/H20/CO2 o.k, according to the manual) It looks as there would be a leak in the injector system, and air diffusion into the system via the upper part of the injector, but I can not find any leak. Has anybody experience with this GC-MS system and any ideas? Thanks!!! Armin Dietrich ****************************************************************************** From: Achim Treumann Date: Tue, 06 May 1997 15:17:38 +0900 Subject: Re: Methods to identify homodimer or homotrimer? Organization: Fujita Health University Dear Lee, I do not think, MS is the method of choice to get the information you need. It is quite controversial whether proteins which associate in a mass spec experiment will do so also in solution. The two methods I would suggest to you are both a little old fashioned... but I have made the experience that they can give nice results: analytical ultracentrifugation and/or light scattering. Please email me if you need references, I might be able to dig them out. Good luck, Achim ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Dr. Achim Treumann Tel. 81-562-93-9381 Division of Biopolymer Science Fax 81-562-93-8832 Inst. Comprehensive Med. Sci. Fujita Health University Toyoake, Aichi 470-11, Japan ****************************************************************************** From: Lutz Kaeseberg Date: Tue, 6 May 1997 11:22:20 +0200 Subject: quadrupole scan on ZAB-HSQ Organization: Uni Leipzig Hallo! I'm a phd-student at leipzig university and I have a little problem. We have a ZAB-HSQ mass spectrometer (VG Analytical) with VG 11-250J data system and PDP11 software. The manual is incomplete and the machine is too old to get a new one. Nobody in our group knows how to scan the quadrupole. Everybody who once knew it is long gone. Now I'd like to scan the quadrupole to make some nice low energy collision experiments or a reaction intermediate scan. Does anyone know how to scan the quadrupole ? It works, I've seen it, you can turn it up and down manually. But I couldn't figure out how to scan. I'm thankful for any advice. Lutz P.S.: if you post to the newsgroup please mail a copy to my email adress *************************************************************************** * * * Lutz Kaeseberg * * Universitaet Leipzig * * Institut fuer Analytische Chemie * * AK Massenspektrometrie * * Linnestrasse 3 * * 04103 Leipzig * * Tel.: +49 341 97 36059 / 36074 * * email: kaeseber@server1.rz.uni-leipzig.de * * * *************************************************************************** ****************************************************************************** From: MasCom@t-online.de (MasCom) Date: 6 May 1997 09:15:35 GMT Subject: MasCom WEB Page Organization: Telekom Online Internet Gateway Our 1st WEB-Page with many spare parts and accessories for mass spectrometer is ready to visit now under: http:/home.t-online.de/home/mascom as it is still under construction, please visit us often. B. Meyer ****************************************************************************** From: Ken Harding Date: Tue, 06 May 1997 12:05:30 -0700 Subject: TLC-FID Organization: SASTECH R&D Morning to all Instrument Manufacturers I am interested in obtaining information regarding a TLC-FID instrument. Brochures and approximate cost(s) of such a system in your configurations would be appreciated. Application of instrument: petrochemical routine and research environment. Thanking you in advance. Ken Harding Sastech Research and Development P.O. Box 1 Sasolburg 9570 Republic of South Africa E-Mail address: analytik@sasol.com Please quote my name in the subject line. ****************************************************************************** From: Jacques Simard Date: Tue, 06 May 1997 08:41:30 -0400 Subject: Learning Electronics can be FUN!!! / Apprendre l'Électronique peut être AMUSANT!!! Organization: Sympatico |¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯| | | |\ | | | | |\ | | |¯¯| |¯¯| | | | | \ | | | | | \ | | | | |\¯ | | | | \| |__| |__| | \| | |__| | \ | |______________________________________| Version française ci-dessous. Would you like to learn electronics? You have alway wondered how it’s working? You want to be able to repair your own electronic devices? Do you think that it’s necessary to go to high school to understand electronics? If you answered yes to one of those questions, don’t search anymore, the Injunior Club is there. Injuniors Club offer the possibility to learn electronics by yourself and for low cost. Based on new technologies, the Injunior Club offers a variety of products that permits you to explore and discover by yourself the wonderfull world of electronics. Soon the Injunior Club will put to the market a serie of CD-ROM that will tech you in simples terms the basics of electronics. The Injunior Club promotes the contact between its members. Each members will have access to a directory of other active members. The member will be able to discuss the difficulties and trouble he encounters with other members. An online service on the internet is also avaliable. The members will be able to consult one of or technician in case of a trouble with one of our product IF YOU WANT TO DISCOVER ELECTRONICS, INJUNIOR CLUB IS FOR YOU!!! INJUNIOR CLUB http://www3.sympatico.ca/ldee/injunior/ mailto:ldee@sympatico.ca |¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯| | | |\ | | | | |\ | | |¯¯| |¯¯| | | | | \ | | | | | \ | | | | |\¯ | | | | \| |__| |__| | \| | |__| | \ | |______________________________________| Êtes-vous intéressés à apprendre l’électronique? Vous avez toujours voulu comprendre comment tel ou tel dispositif fonctionne? Vous voulez être en mesure de réparer vous même vos appareils électronique? Croyez vous qu’il est nécessaire de faire des hautes études pour comprendre l’électronique. Si vous avez répondu oui à une de ces question, ne cherchez plus, le Club Injunior est là. Le Club Injunior vous offre la possibilité d’apprendre l’électronique de manière autodidacte et à peu de frais. Basé sur les nouvelles technologies, le Club Injunior vous offre une gamme de produits qui vous permettrons d’explorer et de découvrir par vous même le merveilleux monde de l’électronique. De plus, le Club Injunior mettra prochainement sur le marché une série de titres multimédia qui vous enseignerons dans des termes simples les principes de l’électronique. En plus, le Club Injunior favorise le contact entre les membres. Chaque membre disposera d’un annuaire ou il pourra trouver une liste des membres actifs, il pourra ainsi discuter avec d’autre membres de ses difficultés et de ses constatations. Un service « en ligne » est également disponible pour les membres qui ont des problèmes techniques avec les produits du club. SI VOUS VOULEZ DÉCOUVRIR L’ÉLECTRONIQUE, LE CLUB INJUNIOR EST LÀ POUR VOUS. |¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯¯| | | |\ | | | | |\ | | |¯¯| |¯¯| | | | | \ | | | | | \ | | | | |\¯ | | | | \| |__| |__| | \| | |__| | \ | |______________________________________| CLUB INJUNIOR http://www3.sympatico.ca/ldee/injunior/ mailto:ldee@sympatico.ca ****************************************************************************** From: altabios@bham.ac.uk (John E. Fox) Date: 6 May 1997 15:04:20 GMT Subject: protein mass spec problem Organization: Alta Bioscience Electrospray Mass Spectrometry on a purified recombinant protein. Why multiple peaks? We have expressed a recombinant subunit of a protein from a photosynthetic bacterium in E.coli. The subunit is soluble and easily purified. It readily binds its substrate, indicating that it is properly folded. It looks good on SDS-PAGE. N-terminal amino acid analysis of the subunit gives a reasonably clear sequence, identical to that predicted from the gene sequence. Analysis of the pure protein by electrospray mass spec. produces four peaks. Peak one corresponds to the mass predicted from the gene sequence (40287). Peak two is the mass of peak one plus 129. Peak three is about 125 mass units larger than peak two. Peak four is about 105 mass units larger still. The heights of peaks one and two are similar, peak three is about half as big, and peak four maybe half as much again. Does anyone know why we might be getting these multiple forms? The protein was purified in Tris buffer, 5mM ammonium sulphate, 1mM dithiothrietol and dialysed against water before mass spec. John Fox ****************************************************************** Alta BioScience Email: altabios@bham.ac.uk School of Biochemistry Phone: 0121-414-5450 The University of Birmingham Fax: 0121-414-3376 Edgbaston, BIRMINGHAM, B15 2TT, UK ****************************************************************************** From: "Mark A. Hanning-Lee" Date: Tue, 06 May 1997 09:42:23 -0700 Subject: Re: HELP!!! Organization: Prodigy Internet Ivan wrote: } } Anyone has experience in using Finnigan MAT 95S mass spectrometer, I am } wondering if it is a tandem one, and the type of ionization it is } capable. Check the Finnigan Web page at http://www.finnigan.com/ and follow the Products link to the MAT 95 S. Best, Mark -- Mark Hanning-Lee, markhl@prodigy.net ****************************************************************************** From: ron@ftc.gov.pe.ca (Ron Skinner) Date: Wed, 7 May 1997 07:33:52 LOCAL Subject: Re: Injector leak? GC3400 / ITS 40 Organization: PEI Food Technology Centre }Can anyone help me? }I have a problem with a "leak" in the injector system of a Varian GC 400, }connected to a Finnigan MAT ITS 40. If I set the flow of the injector }split and septum purge to "normal" flow rates (30 ml He split / 5 ml He }purge), the MS indicates a leak system. (Ratio N2/H20/CO2 o.k, according }to the manual) }Increasing the septum purge to 60 ml, the system seems to be tight. }(Ratio N2/H20/CO2 o.k, according to the manual) It looks as there would }be a leak in the injector system, and air diffusion into the system via }the upper part of the injector, but I can not find any leak. Has anybody }experience with this GC-MS system and any ideas? }Thanks!!! }Armin Dietrich A good trick I learned to determine the source of MS leaks is to buy a can of electronic component cooler spray available at any electronic repair or supply shop. It normally comes with an extension tube which will allow you to focus the spray where you want it. Turn your MS scan on and view the scan region between say 50 AMU and 130 AMU. Most of these sprays have peaks in that range. Spray the places in the GC where you suspect a leak and watch for the peaks to come from the cooler spray. It is usually very transient so you can adjust and try again until the leak is fixed. This has always worked for me even when determining if the trap head is tight. Good luck Ron ****************************************************************************** From: James Carlson Date: Wed, 07 May 1997 07:46:13 -0400 Subject: Re: quadrupole scan on ZAB-HSQ Organization: University of Cincinnati Lutz Kaeseberg wrote: } } Hallo! } I'm a phd-student at leipzig university and I have a little problem. } We have a ZAB-HSQ mass spectrometer (VG Analytical) with VG 11-250J data } system and PDP11 software. } The manual is incomplete and the machine is too old to get a new one. } Nobody in our group knows how to scan the quadrupole. Everybody who once } knew it is long gone. } Now I'd like to scan the quadrupole to make some nice low energy collision } experiments or a reaction intermediate scan. } Does anyone know how to scan the quadrupole ? It works, I've seen it, you } can turn it up and down manually. But I couldn't figure out how to scan. } I'm thankful for any advice. } } Lutz } } P.S.: if you post to the newsgroup please mail a copy to my email adress } *************************************************************************** } * * } * Lutz Kaeseberg * } * Universitaet Leipzig * } * Institut fuer Analytische Chemie * } * AK Massenspektrometrie * } * Linnestrasse 3 * } * 04103 Leipzig * } * Tel.: +49 341 97 36059 / 36074 * } * email: kaeseber@server1.rz.uni-leipzig.de * } * * } *************************************************************************** Have you contacted Micromass (formerly VG (and most recently Fisons)) for assistance? The factory (Manchester, UK) has a wealth of individuals familiar with these older products. ****************************************************************************** From: MasCom@t-online.de (MasCom) Date: 7 May 1997 14:01:43 GMT Subject: MasCom WEB-Page Organization: Telekom Online Internet Gateway Sorry, the location of our WEB Page was written wrong in the last article. The right location is: http://home.t-online.de/home/mascom Bernhard Meyer ****************************************************************************** From: Rod Mathews Date: 7 May 1997 15:41:54 GMT Subject: Job Available Organization: Pharmacia & Upjohn RESEARCH CHEMIST Pharmacia & Upjohn, Inc., is the world's ninth largest pharmaceutical company. We are involved in areas such as Oncology and Infectious, Inflammatory, Central Nervous System, and Metabolic Diseases, to help people live longer and fuller lives. At Pharmacia & Upjohn you can make a difference, not only for the sake of your career, but also for the millions of people around the world whose lives will be enhanced by the solutions you help us provide. The incumbent will work as a member of the mass spectrometry laboratory in Discovery Research. The laboratory is equipped with a variety of modern instruments. Primary responsibilities include the analysis of small organic molecules as well as biological peptides and proteins. The work requires the ability to operate a mass spectrometer and perform routine maintenance, interpret and communicate results with minimal supervision. The successful candidate will have a M.S. in Chemistry or Biochemistry, or B.S. with minimum of two (2) to three (3) years directly related laboratory experience or equivalent; experience with mass spectrometry is required. Pharmacia & Upjohn offers a stimulating, multi-disciplinary research environment in modern facilities at our Pharma Product Center in Kalamazoo. Our region offers an outstanding mix of cultural and recreational pursuits and an attractive cost of living. Excellent salary and benefit packages are provided. If you would like an opportunity to make a difference with Pharmacia & Upjohn, contact us. For confidential consideration, please send your resume and a description of your research experience to: Pharmacia & Upjohn, Recruiting, Ad #970183, 7000 Portage Road, Kalamazoo, MI 49001. If you are responding through the Internet, include your resume as part of your e-mail (not as an attachment) to: recruit@am.pnu.com. Please include the ad number. We are an equal opportunity employer with a commitment to work place diversity. ****************************************************************************** From: bn Date: Wed, 7 May 1997 15:57:32 BST Subject: Help with fatty acid methyl ester analysis Organization: * HELP! I am a Phd student looking at biodiversity in soil micro-organism communities. The expertise to intrepret data from our HP GCMS is no longer available. We have no midi system but use a bacterial acid methyl ester Mix -CP standard and the chem station library Nbs 75k. The method used previously in the group is to go through each peak on a trace and to look at the mass spec and to deduce from this the structure of the molecules. As a biologist I am having problems with this. I would appreciate any advice on how to work out the structure of the molecules, using minimum standard input. Could anyone recommend references, books etc? My PhD depends on me being able to do this, so any help would be very gratefully recieved! ****************************************************************************** From: altabios@bham.ac.uk (John E. Fox) Date: 8 May 1997 12:05:24 GMT Subject: Re: protein mass spec problem Organization: Alta Bioscience In article <5knh8h$qih@usenet.bham.ac.uk>, altabios@bham.ac.uk (John E. Fox) says: Electrospray Mass Spectrometry on a purified recombinant protein. Why multiple peaks? We have expressed a recombinant subunit of a protein from a photosynthetic bacterium in E.coli. The subunit is soluble and easily purified. It readily binds its substrate, indicating that it is properly folded. It looks good on SDS-PAGE. N-terminal amino acid analysis of the subunit gives a reasonably clear sequence, identical to that predicted from the gene sequence. Analysis of the pure protein by electrospray mass spec. produces four peaks. Peak one corresponds to the mass predicted from the gene sequence (40287). Peak two is the mass of peak one plus 129. Peak three is about 125 mass units larger than peak two. Peak four is about 105 mass units larger still. The heights of peaks one and two are similar, peak three is about half as big, and peak four maybe half as much again. Does anyone know why we might be getting these multiple forms? The protein was purified in Tris buffer, 5mM ammonium sulphate, 1mM dithiothrietol and dialysed against water before mass spec. John Fox ****************************************************************** Alta BioScience Email: altabios@bham.ac.uk School of Biochemistry Phone: 0121-414-5450 The University of Birmingham Fax: 0121-414-3376 Edgbaston, BIRMINGHAM, B15 2TT, UK ****************************************************************************** From: "Frederick H. Strobel" Date: Thu, 8 May 1997 08:38:51 -0400 (EDT) Subject: Available VG 11/250 data system for parts Organization: * We have available a VG 11/250 data system; however it is not communicating with the pdp 11. We had it fixed, but it failed within 3 hrs, probably blowing the 659 board. This lead us to a decision to get a new data system. If their is interest in obtaining this data system for the parts, please contact me at fstrobe@emory.edu or at (404)727-6622. Fred Strobel Emory University Mass Spectrometry Center Emory University Atlanta, GA ****************************************************************************** From: "Gary J. Van Berkel" Date: 8 May 1997 16:13:34 GMT Subject: Re: Injector leak? GC3400 / ITS 40 Organization: Lockheed Martin Energy Systems I've had some problems with background air, etc. that looked to be injector related that were traced to leaky solonoid valves for the CI reagent. ****************************************************************************** From: Sung-Chan Jo Date: Fri, 09 May 1997 09:37:22 -0700 Subject: Re: Help with fatty acid methyl ester analysis Organization: SKC R&D Center Hello, I am an analytical chemist working for SKC Ltd. Up to now, I have read the books below and they were very helpful for me. Interpretation of Mass Spectra 4th. ed. by F. W. McLafferty & Frantisek Turecek, 1993, University Science Books Mass Spectrometry for Chemists and Biochemists by Rose & Johnstone, 1982, Cambridge University Press And I found a book very easy to read but contained very good guidelines way back when in a book store. I will add the Title to this list someday soon as I get to find it out again. As you know, much time must be needed to read through the books even though it must be fruitful. So,...attending some short courses held by various Mass Spectrometry Societies must be also a good idea. Hope it helps Sung- Chan Jo bn wrote: } } to go through each peak on a trace and to } look at the mass spec and to deduce from this the structure of the } molecules. As a biologist I am having problems with this. I would } appreciate any advice on how to work out the structure of the molecules, } using minimum standard input. Could anyone recommend references, books } etc? ****************************************************************************** From: Bert van Bavel Date: Fri, 09 May 1997 15:56:01 -0700 Subject: PAH Intercalibration on soil/sediment samples Organization: Kyoto University Dear Group, The Institute of Environmental Chemistry at the Umeå University is arranging an international intercalibration exercise on the analysis of PCDD/DFs in soil and sediments now for the third year. The results and the study design can be found round at the homepage of our Institute at http://www.chem.umu.se/divisions/env/results.htm. This year around 50 labs from around the world are participating. My question to the bioremediation group is if there would be interest to organise a similar study on PAHs on contaminated soils. Other questions are if such a study is already on going or how to get in touch with analytical labs who want to participate in such a study. Interested labs are asked to reply directly to me by @mail. With best regards, Bert van Bavel Institute of Environmental Chemistry Umeå University S-901 87 Umeå Sweden tel:-46 90 166818 fax:-46 90 186155 @mail:bert.vanbavel@chem.umu.se http://www.chem.umu.se/divisions/env/ ****************************************************************************** From: Alain Marineau Date: 11 May 1997 21:53:57 GMT Subject: i need an electromagnet Organization: Alainternet enr. I want to find and electromagnet very strong and that can be ajustable or variable it is possible. Where can i get that or buy that kind of product??? Something that can hold metal piece throw a 12" inch insulated wall of a building. Please reply via e-mail mailto:marineau@geocities.com thanks... ****************************************************************************** From: David Chow Date: 12 May 1997 09:19:37 GMT Subject: Need info. on applications of Mass Spec. Organization: GTE Intelligent Network Services, GTE INS Hi, I am doing a research on the applications of Mass Spec. Does anyone know any site on the web that has the detail information? Thanks for the help. David ****************************************************************************** From: James Carlson Date: Mon, 12 May 1997 08:15:53 -0400 Subject: Re: Need info. on applications of Mass Spec. Organization: University of Cincinnati David Chow wrote: } } Hi, } I am doing a research on the applications of Mass Spec. Does anyone } know any site on the web that has the detail information? Thanks for } the help. } } David Try the web site for the American Society for Mass Spectrometry (http://www.asms.org). There is also a link for the abstracts to the next big meeting (in 3 weeks). Download the abstracts and the session titles and start reading. ****************************************************************************** From: Jennifer Laidlaw Date: Mon, 12 May 1997 10:05:41 -0700 Subject: Post-Doctoral Position, Ph. D. in analytical chemistry, RB96-215 Organization: NRC POST-DOCTORAL POSITION NRC is a dynamic, nationwide R&D organization committed to helping Canada realize its potential as an innovative and competitive nation. Combining our strengths – outstanding people, core science expertise and information, research programs focused on key technologies and technology diffusion – with those of industrial and academic partners, we foster Canada's emerging national system of innovation. Your Challenge To develop sample preparation methodology for optimized LC/MS analysis in environmental matrices. The applicant will work with analytes such as resin acids, surfactants, TPHs, VOCs, BTEX, bitumens, explosives, TCE, PCBs, and their biotransformed products. It is the duty of the applicant to identify the structure of target analytes using the LC/MS method. Expectedly, the applicant will operate the LC/MS system, develop the method, and identify target analytes. Data gained by LC/MS will be used to study the fate of pollutants and their metabolic pathways in environmental processes. The association of the position with environmental research demands from the candidate to work with several matrices including water, air, soil, sediment, sludge and bioslurries. Also, it is expected from the candidate to work with other interrelated techniques such as supercritical fluid extraction (SFE), thermal desorption techniques and soil sorption isotherms, and solid phase micro extraction (SPME). Education Ph.D. in analytical chemistry (preferably in mass spectrometry). Your credentials At least two years experience in environmental analysis and in mass spectrometry, preferably in LC/MS. A security screening will be required. The salary range for this position varies according to qualifications and experience. A relocation costs may be negotiable. To explore this opportunity, send your resume before May 23, 1997 indicating reference number RB96-215, to: Sophie Lanctôt, Human Resources Assistant, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2. NRC is an equal opportunity employer. We thank all those who apply. However, only those selected for further consideration will be contacted. ****************************************************************************** From: Darren Date: Tue, 13 May 1997 09:07:30 +0100 Subject: Modern Nautilus Organization: University of Oxford Hi I'm after some modern Nautilus shells for isotopic analysis. Does anyone know where I can obtain some. Any responses appreciated in advance. Cheers Darren ****************************************************************************** From: Bert van Bavel Date: Tue, 13 May 1997 15:00:07 -0700 Subject: Professor in Environmental Chemistry, Umeå University, Sweden Organization: Umeå University Dear colleagues, As you might have heard we are looking for a new Professor in Environmental Chemistry at the Umeå University. Professor Rappe is going to retire in May 1998. Please forwards this message to scientists who might be interested in the challenge. A job description can be found at http://www.chem.umu.se/divisions/env/prof.htm. More information on the Institute of Environmental Chemistry at http://www.chem.umu.se/divisions/env/ With best regards Dr. Bert van Bavel ****************************************************************************** From: Jacques Simard Date: Tue, 13 May 1997 10:32:55 -0400 Subject: New! A Electronic Development Tool! Organization: Sympatico I would like to introduce you to a new tool : The Digital Experimenter. The Digital Experimenter is a usefull tool that you can use to desing digital circuits and to debug a malfunctionning circuits. The Digital Experimenter includes basic input and display functions that permits the designer to concentrate on the application instead of the display. Display circuits (such as BCD 7 segment displays) can take a lot of space. With the Digital Experimenter, you have four BCD 7 segments displays avaliable so you don't have to bother about the space they use. There's also an 8 bits binary display, two bounce free logic switchwes, 8 data switches, fixed and variable square wave frequency generator. You also have access to four SCHMITT Triggers. As you can see, there's a lot of usefull tools that the designer often needs. All these functions are on a PCB that plugs directly on a beadboard. If you want to know more about this wonderfull tool, come visit our homepage at: http://www3.sympatico.ca/ldee/injueng/ Or email us at: mailto:ldee@sympatico.ca -- ************************************************************ * LDEE Inc. TEL: (819) 566-2807 * * 1080 Galt est Bureau 101 FAX: (819) 566-0427 * * Sherbrooke, Quebec ldee@sympatico.ca * * J1G 4C7 http://www3.sympatico.ca/ldee * ************************************************************ ****************************************************************************** From: lwang@deakin.edu.au (Lipo Wang) Date: 14 May 1997 01:04:19 GMT Subject: PAKDD-98: Call for Papers Organization: School of Computing & Mathematics - Deakin University Distribution: world ====================================================================== C A L L F O R P A P E R S ====================================================================== The Second Pacific-Asia Conference on Knowledge Discovery and Data Mining (PAKDD-98) ---------------------------------------------- Melbourne, Australia, 15-17 April 1998 ====================================== URL: http://www.sd.monash.edu.au/pakdd-98 The Second Pacific-Asia Conference on Knowledge Discovery and Data Mining (PAKDD-98) will provide an international forum for the sharing of original research results and practical development experiences among researchers and application developers from different KDD related areas such as machine learning, databases, statistics, knowledge acquisition, data visualization, software re-engineering, and knowledge-based systems. It will follow the success of PAKDD-97 held in Singapore in 1997 by bringing together participants from universities, industry and government. Papers on all aspects of knowledge discovery and data mining are welcome. Areas of interest include, but are not limited to: - Data and Dimensionality Reduction - Data Mining Algorithms and Tools - Data Mining and Data Warehousing - Data Mining on the Internet - Data Mining Metrics - Data Preprocessing and Postprocessing - Data and Knowledge Visualization - Deduction and Induction in KDD - Discretisation of Continuous Data - Distributed Data Mining - KDD Framework and Process - Knowledge Representation and Acquisition in KDD - Knowledge Reuse and Role of Domain Knowledge - Knowledge Acquisition in Software Re-Engineering and Software Information Systems - Induction of Rules and Decision Trees - Management Issues in KDD - Machine Learning, Statistical and Visualization Aspects of KDD